CN107908205A - Constant temperature observation device - Google Patents
Constant temperature observation device Download PDFInfo
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- CN107908205A CN107908205A CN201711111869.3A CN201711111869A CN107908205A CN 107908205 A CN107908205 A CN 107908205A CN 201711111869 A CN201711111869 A CN 201711111869A CN 107908205 A CN107908205 A CN 107908205A
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- constant temperature
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 94
- 210000001161 mammalian embryo Anatomy 0.000 claims abstract description 48
- 241000252212 Danio rerio Species 0.000 claims abstract description 41
- 238000012360 testing method Methods 0.000 claims abstract description 12
- 238000010438 heat treatment Methods 0.000 claims description 6
- 230000003321 amplification Effects 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 5
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims description 3
- 230000003993 interaction Effects 0.000 claims description 3
- 238000002474 experimental method Methods 0.000 abstract description 20
- 238000004458 analytical method Methods 0.000 abstract description 2
- 230000008859 change Effects 0.000 description 9
- 238000011161 development Methods 0.000 description 7
- 230000018109 developmental process Effects 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- 230000008569 process Effects 0.000 description 5
- 230000004083 survival effect Effects 0.000 description 5
- 230000007613 environmental effect Effects 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 241000251468 Actinopterygii Species 0.000 description 3
- 241000283070 Equus zebra Species 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
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- 238000011160 research Methods 0.000 description 3
- 240000005373 Panax quinquefolius Species 0.000 description 2
- 235000003140 Panax quinquefolius Nutrition 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000012447 hatching Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- 241000448255 Congiopodus peruvianus Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 230000003670 easy-to-clean Effects 0.000 description 1
- 231100000463 ecotoxicology Toxicity 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 230000002070 germicidal effect Effects 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 description 1
- 230000009067 heart development Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000004660 morphological change Effects 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
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- 231100000027 toxicology Toxicity 0.000 description 1
Classifications
-
- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05D—SYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
- G05D23/00—Control of temperature
- G05D23/19—Control of temperature characterised by the use of electric means
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/003—Aquaria; Terraria
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- Automation & Control Theory (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention provides a kind of constant temperature observation device, it is related to the technical field of observation and analysis instrument, including babinet, temperature-adjusting device and observation device;Water bath being provided with babinet, providing isoperibol for the zebrafish embryo in sample holder during for testing;Temperature-adjusting device is located on babinet, for adjusting the water temperature in water bath;Observation device is located at babinet top, and the zebrafish embryo in water bath in sample holder is observed during for testing.Constant temperature observation device provided by the invention can ensure that sample holder is in isoperibol during experimental observation, and the zebrafish embryo in sample holder is in stationary temperature environment, ensure experiment be smoothed out and the accuracy of experimental result.
Description
Technical field
The present invention relates to observation and analysis Instrument technology field, more particularly, to a kind of constant temperature observation device.
Background technology
Zebra fish is a kind of common tropical fresh water fish, because its is small, feeding management is convenient, breeds fast, cost is low etc.
Advantage, it is the same in the most commonly used model animal of field of scientific study to have become as mouse, rat at present.In addition, zebra fish exists
Also there is incomparable advantage in Developmental Biology and the research of compound development toxicity, because zebrafish embryo is from by precise and penetrating son
Fish broken shell is simultaneously freely moved about when only needing 48-72 small, the feed that can be open in three days after broken shell;And embryo is in whole hatching process
In be transparent, the development of its internal structure and organ is high-visible under low-powered microscope.Formation, fin such as eyes
Growth, the growth of fish tail, particularly heart development even heart first time bounce and blood first time perfusion all
It can observe and record.Again because embryo stage is sensitive to the reacting condition of external environment, zebrafish embryo is used extensively
In environmental monitoring and ecotoxicology research.Duration of experiment is relatively short, using zebrafish embryo as experiment material development section
Learn research can effectively save cost and time, and its morphological change is notable, in experimentation it is observed that more
Toxicology terminal such as heart rate, hatching, deformity etc..Low-powered microscope mainly is relied on to the instrument of zebrafish embryo observation at present
And anatomical lens is observed, but it is typically to take out the zebrafish embryo in experiment from culture environment during observation
It is observed in external environment, but external temperature environment is different from experimental temperature condition, the abrupt change of temperature can be to zebra
The development of fish embryo affects, if zebrafish embryo can not be kept according to the temperature that experiment condition needs,
The growth and development of zebrafish embryo, survival condition can be affected, experimental result can be caused it is difficult to predict influence, cause
The otherness of experimental result.
The content of the invention
It is an object of the invention to provide a kind of constant temperature observation device, it can realize in observation process, sample holder is in perseverance
In warm environment, the zebrafish embryo in sample holder is in stationary temperature environment, ensure that experiment be smoothed out and it is real
Test the accuracy of result.
To achieve the above object, the present invention provides following technical scheme:
The present invention provides a kind of constant temperature observation device, including:Babinet, temperature-adjusting device and observation device;
Water bath being provided with the babinet, providing isoperibol for the zebrafish embryo in sample holder during for testing;
The temperature-adjusting device is located on the babinet, for adjusting the water temperature in the water bath;
The observation device is located at the babinet top, for observing the water by amplifying different multiples in experimentation
Zebrafish embryo in bath in sample holder.
Further, the temperature-adjusting device includes temperature sensor, controller and heater;
The temperature sensor, is connected in the water bath and with the controller signals, for gathering the water
Water temperature information in bath, and the water temperature information is sent to the controller;
The controller is used to receive the water temperature information, and when the water temperature information be less than first threshold, generates the
One control command, first control command is sent to the heater, raw when the water temperature information is higher than second threshold
Into the second control command, second control command is sent to the heater;
The heater, is connected in the water bath and with the controller signals, for receiving first control
System order, and work is begun to warm up according to first control command, it is additionally operable to receive second control command, and according to institute
State the second control command and stop heating work.
Further, the controller includes PID controller.
Further, the temperature-adjusting device further includes operation panel, and the operation panel is arranged on the babinet table
Face, and be connected with the controller signals, for human-computer interaction.
Further, the temperature-adjusting device further includes display panel, and the display panel is arranged on the babinet table
Face, and be connected with the controller signals, for showing the water temperature information.
Further, carrier is provided with the water bath, for carrying the sample holder equipped with zebrafish embryo, so that
Sample holder is located in water bath.
Further, making the material of the carrier includes acrylic board.
Further, the observation device includes the camera lens of supporting rack, lens barrel and some different amplifications;
Support frame as described above one end is connected with the babinet, and the other end is connected with the lens barrel;
The camera lens is installed in the lens barrel.
Further, the adjusting part for adjusting the lens barrel height, the adjusting group are provided with support frame as described above
Part is sequentially connected with the lens barrel.
Further, the observation device further includes headlamp, and the headlamp is installed on support frame as described above.
Constant temperature observation device provided by the invention has the advantages that:
The present invention provides a kind of constant temperature observation device, including:Babinet, temperature-adjusting device and observation device;Set in babinet
Water bath being equipped with, providing isoperibol for the zebrafish embryo in sample holder during for testing;Temperature-adjusting device is located at babinet
On, for adjusting the water temperature in water bath;Observation device is located at babinet top, in experimentation by amplifying different multiples
Observe the zebrafish embryo in sample holder in water bath.
In constant temperature observation device provided by the invention, water bath can provide a perseverance for the zebrafish embryo in sample holder
Determine temperature environment, temperature-adjusting device can automatically adjust the water temperature in water bath according to predetermined temperature scope.Using
When observation device observes the state of growing of the zebrafish embryo in sample holder and carries out counting operation to living individuals, spot
Horse fish embryo is in stationary temperature environment, and growth course and survival condition ensure closer to the state in culture environment
The accuracy of observation device observation.
Zebrafish embryo is very sensitive to environmental change, and the method to zebrafish embryo observation is typically in testing at present
Zebrafish embryo take out from culture environment and be observed in external environment, but external temperature environment is different from experiment temperature
Degree condition, the abrupt change of temperature can affect the growth and development of zebrafish embryo, and the change of temperature can also draw
Send out problem a series of, thus in the experiment for making to study for zebrafish embryo if it cannot be guaranteed that experimental temperature condition with
The culture environment temperature conditionss of embryo are identical, and the result of experiment is often inaccurate, also just loses the meaning of experiment.And this
The constant temperature observation device that invention provides can realize in observation process that sample holder is in isoperibol, the zebra in sample holder
Fish embryo is under the isothermal experiments temperature conditionss identical with culture environment temperature conditionss, ensure that experiment be smoothed out and
The accuracy of experimental result.
Brief description of the drawings
, below will be to specific in order to illustrate more clearly of the specific embodiment of the invention or technical solution of the prior art
Embodiment or attached drawing needed to be used in the description of the prior art are briefly described, it should be apparent that, in describing below
Attached drawing is some embodiments of the present invention, for those of ordinary skill in the art, before not making the creative labor
Put, other attached drawings can also be obtained according to these attached drawings.
Fig. 1 is the structure diagram one of constant temperature observation device provided in an embodiment of the present invention;
Fig. 2 is the structure diagram two of constant temperature observation device provided in an embodiment of the present invention;
Fig. 3 is thermostatic system block diagram provided in an embodiment of the present invention.
Icon:1- babinets;2- temperature-adjusting devices;3- observation devices;11- water baths;12- carriers;21- temperature sensing
Device;22- controllers;23- heaters;24- operation panels;25- display panels;31- supporting racks;32- lens barrels;33- camera lenses;34-
Headlamp.
Embodiment
Technical scheme is clearly and completely described below in conjunction with attached drawing, it is clear that described implementation
Example is part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, ordinary skill
Personnel's all other embodiments obtained without making creative work, belong to the scope of protection of the invention.
In the description of the present invention, it is necessary to explanation, term " " center ", " on ", " under ", "left", "right", " vertical ",
The orientation or position relationship of the instruction such as " level ", " interior ", " outer " be based on orientation shown in the drawings or position relationship, merely to
Easy to describe the present invention and simplify description, rather than instruction or imply signified device or element must have specific orientation,
With specific azimuth configuration and operation, therefore it is not considered as limiting the invention.In addition, term " first ", " second ",
" the 3rd " is only used for description purpose, and it is not intended that instruction or hint relative importance.
In the description of the present invention, it is necessary to illustrate, unless otherwise clearly defined and limited, term " installation ", " phase
Even ", " connection " should be interpreted broadly, for example, it may be being fixedly connected or being detachably connected, or be integrally connected;Can
To be mechanical connection or be electrically connected;It can be directly connected, can also be indirectly connected by intermediary, Ke Yishi
Connection inside two elements.For the ordinary skill in the art, with concrete condition above-mentioned term can be understood at this
Concrete meaning in invention.
The embodiment of the present invention is described in detail below in conjunction with attached drawing.It should be appreciated that this place is retouched
The embodiment stated is merely to illustrate and explain the present invention, and is not intended to limit the invention.
Fig. 1, Fig. 2 and Fig. 3 are refer to, constant temperature observation device provided in an embodiment of the present invention is made below in conjunction with attached drawing detailed
Describe in detail bright.
The embodiment provides a kind of constant temperature observation device, including:Babinet 1, temperature-adjusting device 2 and observation dress
Put 3;
Water bath 11 being provided with babinet 1, providing isoperibol for the zebrafish embryo in sample holder during for testing;
Temperature-adjusting device 2 is located on babinet 1, for adjusting the water temperature in water bath 11;
Observation device 3 is located at 1 top of babinet, for being contained in experimentation by amplifying in different multiples observation water bath 11
Zebrafish embryo in sample device.
In the constant temperature observation device that the embodiment of the present invention provides, water bath 11 can be the zebrafish embryo in sample holder
A stationary temperature environment is provided, temperature-adjusting device 2 can automatically adjust water bath according to predetermined temperature scope
Water temperature in 11.Using observation device 3 observe sample holder in embryo state of growing and living individuals are counted
During number operation, zebrafish embryo is in stationary temperature environment, and growth course and survival condition are closer in culture environment
State, ensure that observation device 3 observe accuracy.
Zebrafish embryo is very sensitive to environmental change, and the method to zebrafish embryo observation is typically in testing at present
Zebrafish embryo take out from culture environment and be observed in external environment, but external temperature environment is different from experiment temperature
Degree condition, the abrupt change of temperature can affect the growth and development of zebrafish embryo, and the change of temperature can also draw
Send out problem a series of, thus in the experiment for making to study for zebrafish embryo if it cannot be guaranteed that experimental temperature condition with
The culture environment temperature conditionss of embryo are identical, and the result of experiment is often inaccurate, also just loses the meaning of experiment.And this
The constant temperature observation device that invention provides can realize in observation process that sample holder is in isoperibol, the zebra in sample holder
Fish embryo is under the isothermal experiments temperature conditionss identical with culture environment temperature conditionss, ensure that experiment be smoothed out and
The accuracy of experimental result.
Elaborate below to temperature-adjusting device 2:
Temperature-adjusting device 2 includes temperature sensor 21, controller 22 and heater 23;Wherein:
Temperature sensor 21, is connected in water bath 11 and with 22 signal of controller, for gathering in water bath 11
Water temperature information, and water temperature information is sent to controller 22;
Controller 22 is used to receive water temperature information, and when water temperature information is less than first threshold, generates the first control command,
First control command is sent to heater 23, when water temperature information is higher than second threshold, the second control command is generated, by second
Control command is sent to heater 23;
Heater 23, is connected in water bath 11 and with 22 signal of controller, for receiving the first control command, and root
Work is begun to warm up according to the first control command, is additionally operable to receive the second control command, and stop heating according to the second control command
Work.
The nutrient solution of the survival of appropriate experimental object and growth is filled in the sample holder such as beaker or test tube.The implementation of the present invention
Need the water temperature in water bath 11 maintaining the survival of appropriate experimental object and the temperature grown in the constant temperature observation device that example provides
In the range of degree, to meet the requirement of experiment condition.Temperature sensor 21 in water bath 11 gathers the water in water bath 11
Temperature value, and water temperature value is sent to controller 22;Controller 22 receives the water temperature value of the transmission of temperature sensor 21, can be by the water
Temperature value compared with predetermined temperature scope in controller 22, wherein, predetermined temperature may range from first threshold
To between second threshold;If water temperature value is in the temperature range, controller 22 does not issue control command to heater 23;Work as water
When temperature value is less than first threshold, controller 22 generates the first control command, and the first control command is sent to heater 23, adds
Hot device 23 is begun to warm up according to the first control command;When water temperature value is higher than second threshold, the generation of controller 22 second control life
Order, and the second control command is sent to heater 23, heater 23 and stops heating according to the second control command.
In above-mentioned alternative, controller 22 includes PID controller.PID controller is to whole according to PID control principle
Control system carries out bias adjustment, so that the actual value of temperature controlled variable is consistent with the predetermined value of requirement of experiment.PID control
Device is a common backfeed loop component in Industry Control Application.22 data being collected into of this controller and a ginseng
Examine value to be compared, then this difference is used to calculate new input value, the purpose of this new input value is can to allow be
The data of system reach or are maintained at reference value.Different with other simple control computings, PID controller can be according to history number
Input value is adjusted according to the occurrence rate with difference, can so make system more accurate, is more stablized.
The control loop of temperature-adjusting device 2 includes:Sensor, controller 22 and output equipment;Sensor in circuit
The measurement result that i.e. temperature sensor 21 obtains water temperature value i.e. in water bath 11, controller 22 is made decision to be set by an output
Standby i.e. heater 23 is made a response;Controller 22 obtains measurement result from sensor, then subtracts measurement knot with demand result
Fruit obtains error.Then going out a correction value to system by the use of error calculation can be from it as input results, such system
Output result in eliminate error.For the present embodiment, water bath 11 provides one for the zebrafish embryo in sample holder
Steady temperature environment, the water temperature of water bath 11 need to be maintained at certain temperature, and the temperature sensor 21 in water bath 11 is used for
Check the water temperature in water bath 11, thus obtain measurement result;Controller 22 has a fixed user input values
Represent the water temperature needed in water bath 11;The output equipment of controller 22 can be connected to the switch of control 23 connecting and disconnecting of the circuit of heater
On, closure switch will give the water heating in water bath 11, and disconnecting switch will stop to the water heating in water bath 11, water-bath
Water can gradually cool down with time lengthening in groove 11, and the control signal of this switch is exactly the variable for needing to control, it is also this
The input results of system keep the constant of water temperature in this water bath 11.
In the alternative of the present embodiment, temperature-adjusting device 2 further includes operation panel 24, and operation panel 24 is arranged on case
1 surface of body, and be connected with 22 signal of controller, for human-computer interaction.Specifically, user can be by operation panel 24
Power supply key control temperature-adjusting device 2 open/close states, operation panel 24 can also be passed through and set water temperature in water bath 11
Temperature value.In a kind of embodiment, temperature-adjusting device 2 also has reservation function, and reservation is provided with operation panel 24
Function key and time adjustment key;The reservation function largely improves facility for user.
In the alternative of the present embodiment, temperature-adjusting device 2 further includes display panel 25, and display panel 25 is arranged on case
1 surface of body, and be connected with 22 signal of controller, for showing water temperature information.Specifically, panel can show water bath 11
Interior real-time water temperature value, can also show predetermined temperature scope, and the information such as working time.
In the present embodiment, carrier 12 is provided with water bath 11, for carrying the sample holder equipped with zebrafish embryo, with
Sample holder is set to be located in water bath.
Elaborate below to carrier 12:
Carrier 12 can be used as mould, have the corresponding tank for placing the sample holders such as beaker, test tube on carrier 12, ensure
Sample holder is in water bath.For adaptation variety classes, the sample holder of different model, carrier 12 can be with water bath
11 are flexibly connected using clamping, threaded connection etc., to replace different carriers 12, expand the scope of application.Meanwhile one
The tank of multiple placement sample holders can be provided with carrier 12, the Sheng such as beaker, test tube of different model can be placed at the same time
Sample device.
In the preferable technical solution of the present embodiment, the material for making carrier 12 includes but is not limited to acrylic board.It is sub-
Gram force is also known as the organic glass of specially treated, is organic glass regeneration product, and weather-proof and acid-proof alkaline is good;With other materials system
Condition ratio, long lifespan more than 3 years;Translucency is good, and up to more than 92%, required intensity of light is smaller, saves electric energy;Anti-impact
Hit that power is strong, be 16 times of simple glass, be adapted for mount on the area for especially needing safety;Excellent insulating property, is adapted to various
Electrical equipment;Plasticity is strong, and variation in moulding is big, and machine-shaping is easy;Recovery rate is high, is recognized by the environmental consciousness increasingly strengthened
Together;It is easy to maintain, it is easy to clean.
Elaborate below to observation device 3:
In the present embodiment, observation device 3 includes the camera lens 33 of supporting rack 31, lens barrel 32 and some different amplifications;
31 one end of supporting rack is connected with babinet 1, and the other end is connected with lens barrel 32;
Camera lens 33 is installed in lens barrel 32.
It should be noted that camera lens 33 can replace with difference as needed in constant temperature observation device provided in this embodiment
The camera lens 33 of amplification factor.Simple microscope and the anatomical lens visual field are smaller, and when observing zebrafish embryo, there are certain limitation
Property, and visual field adjustment process is time-consuming and laborious, adds the time of experimental implementation.For this problem, the camera lens in the present embodiment
33 are different from microscopical eyepiece, are specifically as follows the enlarging lens of different amplification, expand the field range of camera lens 33.
In the alternative of the present embodiment, the adjusting part for adjusting 32 height of lens barrel is provided with supporting rack 31, is adjusted
Section component is sequentially connected with lens barrel 32.Specifically, adjusting part can include but are not limited to adjusting knob, gear and rack,
Wherein:Adjusting knob is connected with gear drive, and wheel and rack engagement, rack is sequentially connected with lens barrel 32, passes through rotational support
Adjusting knob on frame 31, adjusting knob band moving gear rotate, and gear drives the rack engaged with gear to move up and down, and realizes mirror
The up and down motion of cylinder 32, and then realize moving towards water bath 11 or being moved away from water bath 11 for camera lens 33.
In the alternative of the present embodiment, observation device 3 further includes headlamp 34, and headlamp 34 is installed on supporting rack 31
On.Headlamp 34 can be used for illuminating during Germicidal efficacy, can be used for providing intensity of illumination living condition for zebrafish embryo.
The constant temperature observation device of the present invention is illustrated above, still, the present invention is not limited to above-mentioned specific real
Mode is applied, without departing from the scope of claim, various deformations or change can be carried out.The present invention is included in right
Various modifications and change in the range of it is required that.
Finally it should be noted that:The above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations;To the greatest extent
Pipe is described in detail invention with reference to foregoing embodiments, it will be understood by those of ordinary skill in the art that:It is still
It can modify to the technical solution described in foregoing embodiments, either which part or all technical characteristic are carried out
Equivalent substitution;And these modifications or replacement, the essence of appropriate technical solution is departed from various embodiments of the present invention technical side
The scope of case.
Claims (10)
- A kind of 1. constant temperature observation device, it is characterised in that including:Babinet (1), temperature-adjusting device (2) and observation device (3);Water bath (11) being provided with the babinet (1), providing constant temperature ring for the zebrafish embryo in sample holder during for testing Border;The temperature-adjusting device (2) is located on the babinet (1), for adjusting the water temperature in the water bath (11);The observation device (3) is located at the babinet (1) top, in experimentation by amplify different multiples observation described in Zebrafish embryo in water bath (11) in sample holder.
- 2. constant temperature observation device according to claim 1, it is characterised in that the temperature-adjusting device (2) includes temperature Sensor (21), controller (22) and heater (23);The temperature sensor (21), is connected in the water bath (11) and with the controller (22) signal, for adopting Collect the water temperature information in the water bath (11), and the water temperature information is sent to the controller (22);The controller (22) is used to receive the water temperature information, and when the water temperature information be less than first threshold, generates the One control command, first control command is sent to the heater (23), is higher than second threshold in the water temperature information When, the second control command is generated, second control command is sent to the heater (23);The heater (23), is connected in the water bath (11) and with the controller (22) signal, for receiving The first control command is stated, and work is begun to warm up according to first control command, is additionally operable to receive second control command, And heating work is stopped according to second control command.
- 3. constant temperature observation device according to claim 2, it is characterised in that the controller (22) includes PID controller.
- 4. constant temperature observation device according to claim 2, it is characterised in that the temperature-adjusting device (2) further includes behaviour Make panel (24), the operation panel (24) is arranged on the babinet (1) surface, and is connected with the controller (22) signal, For human-computer interaction.
- 5. constant temperature observation device according to claim 2, it is characterised in that the temperature-adjusting device (2) further includes aobvious Show panel (25), the display panel (25) is arranged on the babinet (1) surface, and is connected with the controller (22) signal, For showing the water temperature information.
- 6. constant temperature observation device according to claim 1, it is characterised in that be provided with carrier in the water bath (11) (12), for carrying the sample holder equipped with zebrafish embryo, so that sample holder is located in water bath.
- 7. constant temperature observation device according to claim 6, it is characterised in that making the material of the carrier (12) includes Acrylic board.
- 8. constant temperature observation device according to claim 1, it is characterised in that the observation device (3) includes supporting rack (31), lens barrel (32) and the camera lens (33) of some different amplifications;Support frame as described above (31) one end is connected with the babinet (1), and the other end is connected with the lens barrel (32);The camera lens (33) is installed in the lens barrel (32).
- 9. constant temperature observation device according to claim 8, it is characterised in that be provided with support frame as described above (31) for adjusting The adjusting part of the lens barrel (32) height is saved, the adjusting part is sequentially connected with the lens barrel (32).
- 10. constant temperature observation device according to claim 8, it is characterised in that the observation device (3) further includes headlamp (34), the headlamp (34) is installed on support frame as described above (31).
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Citations (12)
Publication number | Priority date | Publication date | Assignee | Title |
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WO1991012888A1 (en) * | 1990-02-28 | 1991-09-05 | Kreatech Biotechnology B.V. | Apparatus for automatically performing a biotechnological process at different desired temperatures |
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CN2537992Y (en) * | 2002-02-05 | 2003-02-26 | 武汉大学 | Biological viewing lens |
CN1928761A (en) * | 2006-08-25 | 2007-03-14 | 中国海洋大学 | Multiple temperature control system for aquatic organisms |
CN202351502U (en) * | 2011-12-22 | 2012-07-25 | 张百全 | Portable organism observation lens |
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