CN106719172A - Zebrafish embryo hatching apparatus are used in experiment - Google Patents
Zebrafish embryo hatching apparatus are used in experiment Download PDFInfo
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- CN106719172A CN106719172A CN201710062404.7A CN201710062404A CN106719172A CN 106719172 A CN106719172 A CN 106719172A CN 201710062404 A CN201710062404 A CN 201710062404A CN 106719172 A CN106719172 A CN 106719172A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The present invention provides a kind of experiment and uses zebrafish embryo hatching apparatus, including：Incubator and the support frame being arranged in incubator, incubator inner bottom part are provided with the primary heater for heated water bath, the first lid are provided with the top of incubator, and light source is provided with the first lid bottom；Support frame includes the second installing plate that the first installing plate and the first installing plate be arranged in parallel, the edge of the first installing plate is connected to form culture dish heating chamber with the edge of the second installing plate by side plate, the mounting hole of multiple array distributions is offered on first installing plate, multiple inlet openings are offered on second installing plate, mounting hole is 5 with the diameter ratio of inlet opening：1-4：1.The present invention is due to using heating water bath, and temperature Relative light intensity incubator is more constant using the temperature of Hot-blast Heating, meanwhile, mounting hole is 5 with the diameter ratio of inlet opening：1-4：1, make the fluctuation of heat exchange between nutrient solution in the water and culture dish in culture dish heating chamber smaller, so that experimental temperature is more constant, experimental result is more reliable.
Description
Technical field
The present invention relates to incubation equipment technical field, more particularly, to a kind of experiment zebrafish embryo hatching apparatus.
Background technology
In recent years, zebra fish has reproduction ability strong, in vitro fertilization and develops because of it, embryo is transparent is easy to observation, property
The advantages of processing maturation period is short, cost is relatively low, is widely applied to the correlation of Developmental Biology, molecular genetics and ecological toxicology
In research.Increasing toxicological experiment research method is certain toxicity using zebrafish embryo exposed to gradient concentration now
Material until its juvenile fish stage, so as to evaluate its toxicology terminal.
Zebra fish is in embryo and juvenile fish stage to the resistivity of temperature change well below adult fish stage.It is used for both at home and abroad
The normal temperature of Zebrafish Embryo be 28.5 DEG C, in the range of 25-33 DEG C embryo still can normal development, beyond this temperature
Degree scope, is incubated will appear from exception for a long time.But in normal development temperature range, even temperature fluctuation by a small margin also can
The physiological functions such as rate of development, consumption rate, heart rate to zebrafish embryo and juvenile fish are impacted.Thus it is guaranteed that identical experiment
During operation temperature it is constant with it is unified, it is possible to increase the accuracy and repeatability of experimental result.
All it is to be placed on being trained in illumination box in existing experimental implementation, when being tested to zebrafish embryo
Support, however, the levels temperature of illumination box has differences, and illumination there is also difference between different layers, therefore, in light
According to carry out embryo experiments in incubator it is difficult to ensure that contrast experiment's group operation temperature and illumination condition it is constant with it is unified, it is impossible to
Ensure the reliability of experimental result, greatly puzzlement is brought to existing experimental study.
The content of the invention
It is an object of the invention to provide a kind of experiment zebrafish embryo hatching apparatus, with solving existing experimental implementation
Using the problem of the experimental result poor reliability of illumination box culture zebrafish embryo.
The present invention provides a kind of experiment and uses zebrafish embryo hatching apparatus, including：Incubator, the incubator inner bottom part sets
The primary heater for heated water bath is equipped with, the first lid is provided with the top of the incubator, at the first lid bottom
Portion is provided with light source；The support frame in the incubator is arranged on, support frame as described above includes the first installing plate and first peace
The second installing plate that dress plate be arranged in parallel, the edge of first installing plate is connected with the edge of second installing plate by side plate
Connect to form culture dish heating chamber, the mounting hole of multiple array distributions, second installing plate are offered on first installing plate
On offer multiple inlet openings, the mounting hole is 5 with the diameter ratio of the inlet opening：1-4：1.
Wherein, each inlet opening is located at outside view field of the mounting hole on second installing plate.
Specifically, the light source is fluorescent lamp, and the mounting hole is multiple rows of, and is set between mounting hole described in per adjacent two row
There is a fluorescent lamp；Or, the mounting hole is multigroup, and every group includes two row's mounting holes, is provided with the middle of every group of mounting hole
One fluorescent lamp.
When actual production is manufactured, the incubator includes hatching portion and air circulation portion, and support frame as described above is placed in described incubating
Change the hatching portion in case, the top in the air circulation portion offers passage.
Preferably, support frame as described above also includes being correspondingly arranged at two supporting legs of the second installing plate bottom, described
Supporting leg is L-shaped, and the top of the L-type vertical portion of the supporting leg is fixedly connected with the bottom of second installing plate.
Further, the experiment zebrafish embryo hatching apparatus also include the water storage being arranged on outside the incubator
Case, is provided with the secondary heater for heated water bath in the storage tank, water inlet is provided with the incubator, described to incubate
Change and be connected by the water inlet between case and the storage tank, the water inlet in the incubator is provided with floating
Ball valve.
Preferably, the primary heater and the secondary heater are heating rod or serpentine heating pipe.
Specifically, the second lid is provided with above the storage tank, is led between second lid and the storage tank
Gemel connection is crossed, gemel connection is passed through between first lid and the incubator.
Further, the hatching lower box part is provided with the first drainpipe, and the water storage lower box part is provided with the second draining
Pipe.
Further, first installing plate, second installing plate and the side plate are made of lucite.
Relative to prior art, the experiment zebrafish embryo hatching apparatus that the present invention is provided have the advantage that：
The present invention provide experiment with zebrafish embryo hatching apparatus when in use, by primary heater in incubator
Water-bath heated, the bath temperature in incubator is maintained at a specific temperature, e.g., may remain in 28.5 DEG C,
And by the light source of the first lid bottom illumination condition good for Zebrafish Embryo is provided.Zebra fish embryo is held by multigroup
The culture dish of tire is placed in the mounting hole of the first installing plate and is contrasted a group development experiment, because contrast groups are placed in same plane
On, it is hereby ensured that the illumination condition that contrast groups receive is identical；During to culture dish using heating water bath, due to peace
Dress hole is 5 with the diameter ratio of inlet opening：1-4：1, therefore, it can to being passed in and out inside support frame from inlet opening in the unit interval
Ratio between the amount of the nutrient solution in water and culture dish is defined such that it is able to limit primary heater to heating water bath
Influence of the temperature fluctuation to water temperature in support frame, and then make heat exchange between nutrient solution in the water and culture dish in support frame
Fluctuation is smaller, more constant to ensure the experimental temperature residing for zebrafish embryo, so that it is guaranteed that illumination and temperature between contrast groups
It is constant with it is unified, make experimental result relatively reliable.
Brief description of the drawings
In order to illustrate more clearly of the specific embodiment of the invention or technical scheme of the prior art, below will be to specific
The accompanying drawing to be used needed for implementation method or description of the prior art is briefly described, it should be apparent that, in describing below
Accompanying drawing is some embodiments of the present invention, for those of ordinary skill in the art, before creative work is not paid
Put, other accompanying drawings can also be obtained according to these accompanying drawings.
Fig. 1 is the structural representation of experiment zebrafish embryo hatching apparatus provided in an embodiment of the present invention；
The structural representation of the experiment zebrafish embryo hatching apparatus that Fig. 2 is provided for another embodiment of the present invention；
Fig. 3 is the top view of experiment zebrafish embryo hatching apparatus provided in an embodiment of the present invention；
Fig. 4 is the section view of incubator and support frame in experiment zebrafish embryo hatching apparatus provided in an embodiment of the present invention
Figure；
Incubator and support frame in the experiment zebrafish embryo hatching apparatus that Fig. 5 is provided for another embodiment of the present invention
Sectional view；
Fig. 6 is the structural representation of support frame in experiment zebrafish embryo hatching apparatus provided in an embodiment of the present invention；
The structural representation of support frame in the experiment zebrafish embryo hatching apparatus that Fig. 7 is provided for another embodiment of the present invention
Figure；
Fig. 8 is the upward view of support frame in experiment zebrafish embryo hatching apparatus provided in an embodiment of the present invention.
Icon：1- incubators；11- primary heaters；The lids of 12- first；121- fluorescent lamps；13- ball-cock assemblys；
The drainpipes of 14- first；15- passages；2- support frames；The installing plates of 21- first；211- mounting holes；22- second is installed
Plate；221- inlet openings；23- side plates；24- supporting legs；3- storage tanks；31- secondary heaters；The drainpipes of 32- second；
The lids of 33- second.
Specific embodiment
Technical scheme is clearly and completely described below in conjunction with accompanying drawing, it is clear that described implementation
Example is a part of embodiment of the invention, rather than whole embodiments.Based on the embodiment in the present invention, ordinary skill
The every other embodiment that personnel are obtained under the premise of creative work is not made, belongs to the scope of protection of the invention.
In the description of the invention, it is necessary to explanation, term " " center ", " on ", D score, "left", "right", " vertical ",
The orientation or position relationship of the instruction such as " level ", " interior ", " outward ", "front", "rear" are to be closed based on orientation shown in the drawings or position
System, is for only for ease of the description present invention and is described with simplified, must have rather than the device or element for indicating or imply meaning
Specific orientation, with specific azimuth configuration and operation, therefore be not considered as limiting the invention.Additionally, term " the
One ", " second ", " the 3rd " are only used for describing purpose, and it is not intended that indicating or implying relative importance.
In the description of the invention, it is necessary to illustrate, unless otherwise clearly defined and limited, term " installation ", " phase
Company ", " connection " should be interpreted broadly, for example, it may be being fixedly connected, or being detachably connected, or be integrally connected；Can
Being to mechanically connect, or electrically connect；Can be joined directly together, it is also possible to be indirectly connected to by intermediary, Ke Yishi
Two connections of element internal.For the ordinary skill in the art, with concrete condition above-mentioned term can be understood at this
Concrete meaning in invention.
As shown in figures 1 to 6, the experiment zebrafish embryo hatching apparatus that the present embodiment is provided, including：Incubator 1, hatching
The inner bottom part of case 1 is provided with the primary heater 11 for heated water bath, and the top of incubator 1 is provided with the first lid 12,
The bottom of one lid 12 is provided with light source；It is arranged on the support frame 2 in incubator 1, support frame 2 includes the first installing plate 21 and the
The edge of the second installing plate 22 that one installing plate 21 be arranged in parallel, the edge of the first installing plate 21 and the second installing plate 22 passes through side
The connection of plate 23 forms culture dish heating chamber, and the mounting hole 211 of multiple array distributions is offered on the first installing plate 21, and second installs
Multiple inlet openings 221 are offered on plate 22, mounting hole 211 is 5 with the diameter ratio of inlet opening 221：1-4：1.
Support frame 2 can make according to following size：The length of the first installing plate 21 and the second installing plate 22 be 60cm,
Width is 60cm, and 100 mounting holes of a diameter of 4.5cm 211 of array distribution form 10 rows 10 and arrange on the first installing plate 21, the
100 inlet openings of diameter 1cm 221 of array distribution form 10 rows 10 and arrange on two installing plates 22, by the edge of the first installing plate 21
It is connected to form culture dish heating chamber by the side plate 23 of a height of 3cm with the edge of the second installing plate 22.
Relative to prior art, the experiment zebrafish embryo hatching apparatus that the present embodiment is provided have the advantage that：
The present invention provide experiment with zebrafish embryo hatching apparatus when in use, by primary heater 11 to incubator
Water-bath in 1 is heated, and the bath temperature in incubator 1 is maintained at a specific temperature, e.g., may remain in 28.5
DEG C, and by the light source of the bottom of the first lid 12 illumination condition good for Zebrafish Embryo is provided.Spot is held by multigroup
The culture dish of horse fish embryo is placed in the mounting hole 211 of the first installing plate 21 and is contrasted a group development experiment, because contrast groups are put
In on same plane, it is hereby ensured that the illumination condition that contrast groups receive is identical；The mistake of heating water bath is being used to culture dish
Cheng Zhong, because the diameter ratio of mounting hole 211 and inlet opening 221 is 5：1-4：1, therefore, it can in the unit interval from inlet opening
Ratio between the amount of the nutrient solution in water and culture dish inside 221 turnover support frames 2 is defined such that it is able to limit
Influence of the primary heater 11 to the temperature fluctuation of heating water bath to water temperature in support frame 2, and then make the water in support frame 2 and training
The fluctuation for supporting heat exchange between nutrient solution in ware is smaller, more constant to ensure the experimental temperature residing for zebrafish embryo, so that
Ensure illumination and the constant and unification of temperature between contrast groups, make experimental result relatively reliable.
Wherein, in order that the water in incubator 1 can preferably pass in and out culture dish heating chamber, so that it is guaranteed that culture dish is heated
Water in chamber can remain 28.5 DEG C, to meet the incubation temperature of zebrafish embryo, the experiment spot that the present embodiment is provided
In the embryo hatching device of horse fish, as shown in figs 6-8, each inlet opening 221 is located at throwing of the mounting hole 211 on the second installing plate 22
Outside the domain of shadow zone.
It is so designed that, it can be ensured that the culture dish that will hold zebrafish embryo is placed in when in mounting hole 211, and culture dish is all the time
Do not contacted with inlet opening 221, water is limited in culture dish heating chamber such that it is able to avoid culture dish bottom from blocking inlet opening 221
Situation about being flowed freely between incubator 1, so that water can be freed in and out in culture dish heating chamber with incubator 1, with
Ensure that the water temperature in support frame 2 remains 28.5 DEG C, and then may insure that zebrafish embryo can normally be incubated, tie experiment
Fruit is more accurate.
Specifically, in order to meet the illumination condition needed for Zebrafish Embryo, zebra is used in the experiment that the present embodiment is provided
In the embryo hatching device of fish, as illustrated in figures 4-5, light source can be fluorescent lamp 121, and mounting hole 211 is multiple rows of, and per adjacent two
A fluorescent lamp 121 is provided between row's mounting hole 211；Or, mounting hole 211 is multigroup, and every group includes two row's mounting holes 211,
A fluorescent lamp 121 is provided with the middle of every group of mounting hole 211.Such that it is able to provide good illumination bar for zebrafish embryo is incubated
Part, to ensure that zebrafish embryo be able to can be smoothed out with normal incubation, experiment.
Herein it should be added that, light source can also be a fluorescent lamp 121 for being arranged on the bottom of the first lid 12.
Certainly, light source can also be area source, such as LED, so that it is guaranteed that to the illumination condition of each position on the first installing plate 21
It is identical, to ensure the accuracy of contrast and experiment.
When actual production is manufactured, in order to meet the oxygen needed for zebrafish embryo is incubated, to ensure that zebrafish embryo can
Normal to be incubated, with zebrafish embryo hatching apparatus, as shown in Figure 1-2, incubator 1 includes incubating for the experiment that the present embodiment is provided
Change portion and air circulation portion, the hatching portion that support frame 2 is placed in incubator 1, the top in air circulation portion offer passage 15.
It is so designed that, outside air can be made to flow into air circulation portion by passage 15, then flowed into from air circulation portion
Hatching portion, to realize exchanging for hatching portion and outside air, is incubated demand to oxygen, so that really for meeting zebrafish embryo
Protecting experiment can be normally carried out, and cause air to free in and out and incubate to avoid the first lid 12 from covering whole incubator 1
Change case 1, the situation for causing zebrafish embryo to be normally incubated.
Further, in order to ensure water can the swimmingly free flow between incubator 1 and culture dish heating chamber, with true
The water temperature protected in culture dish heating chamber can all the time be in 28.5 DEG C, and then ensure zebrafish embryo all the time in isoperibol,
With in zebrafish embryo hatching apparatus, as Figure 4-Figure 6, support frame 2 can also include that correspondence sets for the experiment that the present embodiment is provided
Two supporting legs 24 in the bottom of the second installing plate 22 are put, supporting leg 24 is L-shaped, the top of the L-type vertical portion of supporting leg 24
Bottom with the second installing plate 22 is fixedly connected.
Supporting leg 24 is designed into l-shaped, it can be ensured that 24 pairs of the first installing plates 21 of supporting leg, the second installing plate 22 and side plate
The stationarity of 23 supports, and the bottom of the second installing plate 22 is correspondingly arranged two supporting legs 24, the second installing plate 22 can be made
From the bottom a certain distance of incubator 1 so that water can flow more smooth between incubator 1 and culture dish heating chamber, enter
And ensure the water temperature in culture dish heating chamber and kept for 28.5 DEG C.
Wherein, in order to further ensure that water temperature is constant in incubator 1, make zebrafish embryo all the time in 28.5 DEG C of constant temperature
Be incubated under environment, to ensure the accuracy of experimental result, the experiment that the present embodiment is provided with zebrafish embryo hatching apparatus, such as
Shown in Fig. 1, experiment zebrafish embryo hatching apparatus also include the storage tank 3 being arranged on outside incubator 1, are set in storage tank 3
Have the secondary heater 31 for heated water bath, water inlet be provided with incubator 1, pass through between incubator 1 and storage tank 3 into
The mouth of a river is connected, and the water inlet in incubator 1 is provided with ball-cock assembly 13.
In being studied in toxicological experiment, zebrafish embryo usually needs to hatch several days to determine toxicant to zebra fish
Temperature in embryo hatching influence, and incubator 1 is in 28.5 DEG C all the time, and the moist part in incubator 1 certainly will be caused to steam
Hair.In order to ensure the water-bath in incubator 1 maintains certain altitude, the height of water-bath in incubator 1 is set to be consistently higher than in culture dish
The position reached the standard grade of liquid, to ensure that water-bath preferably can occur heat exchange with the nutrient solution in culture dish, make zebra fish embryo
Tire is all the time under isoperibol, it is desirable to reach above-mentioned requirements, then need in time to make-up water source in incubator 1.But, if
Directly will be unable to ensure the isoperibol in incubator 1 to pouring into cold water in incubator 1, and need in experimentation it is artificial often
Observation water level, it is fairly cumbersome.
Therefore, by the interior secondary heater 31 being provided with for heated water bath of storage tank 3, it can be ensured that storage tank 3
Interior water is in 28.5 DEG C all the time, and by being provided with water inlet on incubator 1, pass through between incubator 1 and storage tank 3 into
The mouth of a river is connected, and the water inlet in incubator 1 is provided with ball-cock assembly 13, after the water in incubator 1 vapors away a part,
The ball float of ball-cock assembly 13 sinks, ball-cock assembly 13 is opened so that the water in storage tank 3 flows into incubator 1, the water in incubator 1
After rising to certain altitude, ball float floats, ball-cock assembly 13 is closed, so that the culture dish for holding zebrafish embryo is in one all the time
In determining the water-bath of height, it is ensured that zebrafish embryo is in isoperibol.In this way to make-up water source in incubator 1 more
Releive, it can be ensured that water-bath remains steady temperature in incubator 1, and without manually often observing water level, improves experiment people
The operating efficiency of member.
Preferably, in order to ensure primary heater 11, secondary heater 31 can respectively in incubator 1 and storage tank 3
Water-bath heated, and water temperature can be maintained required temperature, the experiment that the present embodiment is provided is hatched with zebrafish embryo
In device, as shown in Fig. 1 and Fig. 4-5, primary heater 11 and secondary heater 31 are heating rod or serpentine heating pipe.
Heating rod or serpentine heating pipe in incubator 1 can be placed in the water-bath in air circulation portion, it is also possible to be placed in
In the water-bath of the lower section of support frame 2, heat is produced by heating rod or serpentine heating pipe, make heating water bath, maintain a constant temperature
Degree, to meet experiment demand.
Specifically, the development of experiment, the experiment that the present embodiment is provided are influenceed in order to avoid other debris fall into storage tank 3
With in zebrafish embryo hatching apparatus, as Figure 1-3, the top of storage tank 3 is provided with the second lid 33, the second lid 33
By gemel connection between storage tank 3, gemel connection is passed through between the first lid 12 and incubator 1.
Second lid 33 is set by the top of storage tank 3, when testing, the second lid 33 can be covered in storage tank 3
Top, experiment is influenceed to avoid debris from falling into storage tank 3.Gemel connection will be passed through between second lid 33 and storage tank 3,
By gemel connection between first lid 12 and incubator 1, experimenter is set to be more convenient to open the first lid 12, the second lid 33
Open and close, and then facilitate experimental implementation.
Further, the cleaning for the ease of experimenter to incubator 1 and storage tank 3, the experiment that the present embodiment is provided
With in zebrafish embryo hatching apparatus, as Figure 1-3, the bottom of incubator 1 is provided with the first drainpipe 14, the bottom of storage tank 3
It is provided with the second drainpipe 32.
When experimenter cleans to incubator 1 and storage tank 3, plug on the first drainpipe 14 and the need to be only opened
Plug on two drainpipes 32, the sewage after cleaning is discharged by the first drainpipe 14, the second drainpipe 32, and need not will be whole
Individual device has been removed, has been fallen except sewage, such that it is able to reduce the workload of experimenter.
Wherein, in order to further improve lighting effect of the light source to zebrafish embryo, and it is easy to experimenter to zebra fish
The observation of water level in embryonic development situation and culture dish heating chamber, the first installing plate 21, the second installing plate 22 and side plate 23 can be with
It is made of lucite.
First installing plate 21, the second installing plate 22 and side plate 23 are made of lucite, because lucite has
Certain transparency, therefore lighting effect of the light source to zebrafish embryo can be improved, and it is easy to experimenter to add culture dish
The observation of situation in hot chamber.
Certainly, for the ease of the control preferably to the inner light source of incubator 1, incubator 1 should be impermeable using stainless steel etc.
Bright material is made, to avoid external light source from impacting experiment.
Finally it should be noted that：Various embodiments above is merely illustrative of the technical solution of the present invention, rather than its limitations；To the greatest extent
Pipe has been described in detail with reference to foregoing embodiments to the present invention, it will be understood by those within the art that：Its according to
The technical scheme described in foregoing embodiments can so be modified, or which part or all technical characteristic are entered
Row equivalent；And these modifications or replacement, the essence of appropriate technical solution is departed from various embodiments of the present invention technology
The scope of scheme.
Claims (10)
1. zebrafish embryo hatching apparatus are used in a kind of experiment, it is characterised in that including：
Incubator, the incubator inner bottom part is provided with the primary heater for heated water bath, is set at the top of the incubator
The first lid is equipped with, the first lid bottom is provided with light source；
The support frame in the incubator is arranged on, support frame as described above includes that the first installing plate is parallel with first installing plate
The second installing plate for setting, the edge of first installing plate is connected to form training with the edge of second installing plate by side plate
Ware heating chamber is supported, the mounting hole of multiple array distributions is offered on first installing plate, offered on second installing plate
Multiple inlet openings, the mounting hole is 5 with the diameter ratio of the inlet opening：1-4：1.
2. zebrafish embryo hatching apparatus are used in experiment according to claim 1, it is characterised in that each inlet opening is located at
Outside view field of the mounting hole on second installing plate.
3. zebrafish embryo hatching apparatus are used in experiment according to claim 1, it is characterised in that the light source includes multiple
Fluorescent lamp, wherein：
The mounting hole is multiple rows of, and is provided with a fluorescent lamp between mounting hole described in per adjacent two row；Or,
The mounting hole is multigroup, and every group includes two row's mounting holes, and a fluorescent lamp is provided with the middle of every group of mounting hole.
4. zebrafish embryo hatching apparatus are used in experiment according to claim 1, it is characterised in that the incubator includes incubating
Change portion and air circulation portion, the hatching portion that support frame as described above is placed in the incubator, the top in the air circulation portion
Offer passage.
5. zebrafish embryo hatching apparatus are used in experiment according to claim 1, it is characterised in that support frame as described above also includes
Two supporting legs of the second installing plate bottom are correspondingly arranged at, the supporting leg is L-shaped, and the L-type of the supporting leg is vertical
Partial top is fixedly connected with the bottom of second installing plate.
6. zebrafish embryo hatching apparatus are used in experiment according to claim 1, it is characterised in that also described including being arranged on
Storage tank outside incubator, is provided with the secondary heater for heated water bath in the storage tank, set on the incubator
There is water inlet, be connected by the water inlet between the incubator and the storage tank, described in the incubator
Water inlet is provided with ball-cock assembly.
7. it is according to claim 6 experiment use zebrafish embryo hatching apparatus, it is characterised in that the primary heater with
The secondary heater is heating rod or serpentine heating pipe.
8. zebrafish embryo hatching apparatus are used in experiment according to claim 6, it is characterised in that the top of the storage tank
The second lid is provided with, by gemel connection between second lid and the storage tank, first lid is incubated with described
Pass through gemel connection between change case.
9. zebrafish embryo hatching apparatus are used in experiment according to claim 6, it is characterised in that the hatching lower box part sets
The first drainpipe is equipped with, the water storage lower box part is provided with the second drainpipe.
10. zebrafish embryo hatching apparatus are used in experiment according to claim 1, it is characterised in that first installing plate,
Second installing plate and the side plate are made of lucite.
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| CN107908205A (en) * | 2017-11-13 | 2018-04-13 | 浙江师范大学 | Constant temperature observation device |
| CN108982826A (en) * | 2018-09-28 | 2018-12-11 | 广东工业大学 | Test device and test method of a kind of zebra fish detection nano particle to embryotoxicity |
| CN109329139A (en) * | 2018-12-12 | 2019-02-15 | 上海海洋大学 | A kind of zebra fish roe hatching device |
| CN111849772A (en) * | 2020-07-31 | 2020-10-30 | 中国科学院生态环境研究中心 | Device and method for continuous culture and observation of model fish embryos |
| CN112273333A (en) * | 2020-12-15 | 2021-01-29 | 竹溪县隆源大鲵生物开发有限公司 | Artificial breeding insemination frame for giant salamanders |
| CN114391500A (en) * | 2022-01-13 | 2022-04-26 | 上海海圣生物实验设备有限公司 | Marine shellfish culture system |
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| CN111849772A (en) * | 2020-07-31 | 2020-10-30 | 中国科学院生态环境研究中心 | Device and method for continuous culture and observation of model fish embryos |
| CN111849772B (en) * | 2020-07-31 | 2022-08-26 | 中国科学院生态环境研究中心 | Device and method for continuous culture and observation of model fish embryos |
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