CN206423360U - Zebrafish embryo hatching apparatus is used in experiment - Google Patents

Zebrafish embryo hatching apparatus is used in experiment Download PDF

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Publication number
CN206423360U
CN206423360U CN201720103485.6U CN201720103485U CN206423360U CN 206423360 U CN206423360 U CN 206423360U CN 201720103485 U CN201720103485 U CN 201720103485U CN 206423360 U CN206423360 U CN 206423360U
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CN
China
Prior art keywords
installing plate
incubator
zebrafish embryo
mounting hole
hatching apparatus
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Expired - Fee Related
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CN201720103485.6U
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Chinese (zh)
Inventor
孙洪杰
洪华嫦
张瑜
宋春浩
陈建荣
林红军
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Zhejiang Normal University CJNU
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Zhejiang Normal University CJNU
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Priority to CN201720103485.6U priority Critical patent/CN206423360U/en
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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Abstract

The utility model provides a kind of experiment and uses zebrafish embryo hatching apparatus, including:Incubator and the support frame being arranged in incubator, incubator inner bottom part are provided with the primary heater for heated water bath, and the top of incubator is provided with the first lid, light source is provided with the first lid bottom;Support frame includes the first installing plate, the second installing plate being be arranged in parallel with the first installing plate, the edge of first installing plate is connected to form culture dish heating chamber with the edge of the second installing plate by side plate, the mounting hole of multiple array distributions is offered on first installing plate, offer multiple inlet openings on second installing plate, the diameter ratio of mounting hole and inlet opening is 5:1-4:1.The utility model is due to using heating water bath, and temperature Relative light intensity incubator is more constant using the temperature of Hot-blast Heating, meanwhile, the diameter ratio of mounting hole and inlet opening is 5:1-4:1, make the fluctuation of heat exchange between nutrient solution in the water and culture dish in culture dish heating chamber smaller, so that experimental temperature is more constant, experimental result is more reliable.

Description

Zebrafish embryo hatching apparatus is used in experiment
Technical field
The utility model is related to incubation equipment technical field, is hatched more particularly, to a kind of experiment with zebrafish embryo and filled Put.
Background technology
In recent years, zebra fish has reproduction ability strong, in vitro fertilization and developed because of it, embryo is transparent is easy to observation, property The advantages of processing maturation period is short, cost is relatively low, is widely applied to the correlation of Developmental Biology, molecular genetics and ecological toxicology In research.Increasing toxicological experiment research method is certain toxicity that gradient concentration is exposed to using zebrafish embryo now Material is up to its juvenile fish stage, so as to evaluate its toxicology terminal.
Zebra fish is in embryo and juvenile fish stage to the resistivity of temperature change well below adult fish stage.It is used for both at home and abroad The normal temperature of Zebrafish Embryo be 28.5 DEG C, in the range of 25-33 DEG C embryo still can normal development, beyond this temperature Scope is spent, is incubated will appear from exception for a long time.But in normal development temperature range, even temperature fluctuation by a small margin also can The physiological functions such as the rate of development of zebrafish embryo and juvenile fish, consumption rate, heart rate are impacted.Thus it is guaranteed that identical experiment During operation temperature it is constant with uniformly, it is possible to increase the accuracy and repeatability of experimental result.
All it is to be placed on training in illumination box in existing experimental implementation, when being tested to zebrafish embryo Support, however, the levels temperature of illumination box has differences, and illumination is there is also difference between different layers, therefore, in light According in incubator carry out embryo experiments it is difficult to ensure that contrast experiment group operation temperature and illumination condition it is constant with uniformly, it is impossible to Ensure the reliability of experimental result, great puzzlement is brought to existing experimental study.
Utility model content
The purpose of this utility model is to provide a kind of experiment zebrafish embryo hatching apparatus, grasped with solving existing experiment The problem of experimental result poor reliability of illumination box culture zebrafish embryo is used in work.
The utility model provides a kind of experiment and uses zebrafish embryo hatching apparatus, including:Bottom in incubator, the incubator Portion is provided with the top of the primary heater for heated water bath, the incubator and is provided with the first lid, in the described first lid Body bottom is provided with light source;It is arranged on the support frame in the incubator, support frame as described above includes the first installing plate, with described the The second installing plate that one installing plate be arranged in parallel, the edge at the edge of first installing plate and second installing plate passes through side Plate is connected to form culture dish heating chamber, and the mounting hole of multiple array distributions, second peace are offered on first installing plate Fill and multiple inlet openings are offered on plate, the diameter ratio of the mounting hole and the inlet opening is 5:1-4:1.
Wherein, each inlet opening is located at outside view field of the mounting hole on second installing plate.
Specifically, the light source is fluorescent lamp, and the mounting hole is multiple rows of, and set between mounting hole described in per adjacent two row There is a fluorescent lamp;Or, the mounting hole is multigroup, and every group includes being provided with the middle of two row's mounting holes, every group of mounting hole One fluorescent lamp.
When actual production is manufactured, the incubator includes hatching portion and air circulation portion, and support frame as described above is placed in described incubate Change the hatching portion in case, the top in the air circulation portion offers passage.
Preferably, support frame as described above also includes two supporting legs for being correspondingly arranged at the second installing plate bottom, described Supporting leg is L-shaped, and the top of the L-type vertical portion of the supporting leg is fixedly connected with the bottom of second installing plate.
Further, the experiment zebrafish embryo hatching apparatus also includes the water storage being arranged on outside the incubator It is provided with case, the storage tank on the secondary heater for heated water bath, the incubator and is provided with water inlet, it is described to incubate Change and be connected between case and the storage tank by the water inlet, the water inlet in the incubator is provided with floating Ball valve.
Preferably, the primary heater and the secondary heater are heating rod or serpentine heating pipe.
Specifically, the second lid is provided with above the storage tank, is led between second lid and the storage tank Gemel connection is crossed, gemel connection is passed through between first lid and the incubator.
Further, the hatching lower box part is provided with the first drainpipe, and the water storage lower box part is provided with the second draining Pipe.
Further, first installing plate, second installing plate and the side plate are made of lucite.
Relative to prior art, the experiment that the utility model is provided is had the advantage that with zebrafish embryo hatching apparatus:
The utility model provide experiment with zebrafish embryo hatching apparatus when in use, by primary heater to hatching Water-bath in case is heated, and the bath temperature in incubator is maintained at a specific temperature, e.g., be may remain in 28.5 DEG C, and good illumination condition is provided for Zebrafish Embryo by the light source of the first lid bottom.Zebra is held by multigroup The culture dish of fish embryo, which is placed in the mounting hole of the first installing plate, is contrasted a group development experiment, because contrast groups are placed in same put down On face, it is hereby ensured that the illumination condition that contrast groups receive is identical;During heating water bath is used to culture dish, due to Mounting hole and the diameter of inlet opening ratio are 5:1-4:1, it therefore, it can to being passed in and out in the unit interval from inlet opening inside support frame Water and culture dish in nutrient solution amount between ratio be defined, water-bath is added so as to limit primary heater Influence of the temperature fluctuation of heat to water temperature in support frame, and then make heat exchange between nutrient solution in the water and culture dish in support frame Fluctuation it is smaller, to ensure that the experimental temperature residing for zebrafish embryo is more constant, so that it is guaranteed that illumination and temperature between contrast groups That spends is constant with uniformly, making experimental result relatively reliable.
Brief description of the drawings
, below will be right in order to illustrate more clearly of the utility model embodiment or technical scheme of the prior art The accompanying drawing used required in embodiment or description of the prior art is briefly described, it should be apparent that, describe below In accompanying drawing be some embodiments of the present utility model, for those of ordinary skill in the art, do not paying creativeness On the premise of work, other accompanying drawings can also be obtained according to these accompanying drawings.
The structural representation for the experiment zebrafish embryo hatching apparatus that Fig. 1 provides for the utility model embodiment;
The structural representation for the experiment zebrafish embryo hatching apparatus that Fig. 2 provides for another embodiment of the utility model;
The top view for the experiment zebrafish embryo hatching apparatus that Fig. 3 provides for the utility model embodiment;
Incubator and support frame in the experiment zebrafish embryo hatching apparatus that Fig. 4 provides for the utility model embodiment Sectional view;
Incubator and support in the experiment zebrafish embryo hatching apparatus that Fig. 5 provides for another embodiment of the utility model The sectional view of frame;
The structural representation of support frame in the experiment zebrafish embryo hatching apparatus that Fig. 6 provides for the utility model embodiment Figure;
The structure of support frame in the experiment zebrafish embryo hatching apparatus that Fig. 7 provides for another embodiment of the utility model Schematic diagram;
The upward view of support frame in the experiment zebrafish embryo hatching apparatus that Fig. 8 provides for the utility model embodiment.
Icon:1- incubators;11- primary heaters;The lids of 12- first;121- fluorescent lamps;13- ball-cock assemblys; The drainpipes of 14- first;15- passages;2- support frames;The installing plates of 21- first;211- mounting holes;22- second is installed Plate;221- inlet openings;23- side plates;24- supporting legs;3- storage tanks;31- secondary heaters;The drainpipes of 32- second; The lids of 33- second.
Embodiment
The technical solution of the utility model is clearly and completely described below in conjunction with accompanying drawing, it is clear that described Embodiment is a part of embodiment of the utility model, rather than whole embodiments.Based on the embodiment in the utility model, sheet The every other embodiment that field those of ordinary skill is obtained under the premise of creative work is not made, belongs to this practicality Novel protected scope.
, it is necessary to explanation in description of the present utility model, term " " center ", " on ", " under ", it is "left", "right", " perpendicular Directly ", the orientation or position relationship of the instruction such as " level ", " interior ", " outer ", "front", "rear" are based on orientation shown in the drawings or position Relation is put, description the utility model is for only for ease of and simplifies description, rather than indicate or imply signified device or element There must be specific orientation, with specific azimuth configuration and operation, therefore it is not intended that to limitation of the present utility model.This Outside, term " first ", " second ", " the 3rd " are only used for describing purpose, and it is not intended that indicating or implying relative importance.
, it is necessary to which explanation, unless otherwise clearly defined and limited, term " are pacified in description of the present utility model Dress ", " connected ", " connection " should be interpreted broadly, for example, it may be fixedly connected or be detachably connected, or integratedly Connection;Can be mechanical connection or electrical connection;Can be joined directly together, can also be indirectly connected to by intermediary, It can be the connection of two element internals.For the ordinary skill in the art, above-mentioned art can be understood with concrete condition Concrete meaning of the language in the utility model.
As shown in figures 1 to 6, the experiment zebrafish embryo hatching apparatus that the present embodiment is provided, including:Incubator 1, hatching The inner bottom part of case 1 is provided with the primary heater 11 for heated water bath, and the top of incubator 1 is provided with the first lid 12, The bottom of one lid 12 is provided with light source;It is arranged on the support frame 2 in incubator 1, support frame 2 includes the first installing plate 21, with the The edge of the second installing plate 22 that one installing plate 21 be arranged in parallel, the edge of the first installing plate 21 and the second installing plate 22 passes through side Plate 23 connects the mounting hole 211 for being formed and multiple array distributions being offered on culture dish heating chamber, the first installing plate 21, and second installs Offer multiple inlet openings 221 on plate 22, the diameter ratio of mounting hole 211 and inlet opening 221 is 5:1-4:1.
Support frame 2 can make according to following size:The length of first installing plate 21 and the second installing plate 22 be 60cm, Width is that 10 rows 10 of formation of mounting hole 211 of 100 a diameter of 4.5cm of array distribution on 60cm, the first installing plate 21 are arranged, the 100 diameter 1cm of array distribution inlet opening 221 forms 10 rows 10 and arranged on two installing plates 22, by the edge of the first installing plate 21 It is connected to form culture dish heating chamber by a height of 3cm side plate 23 with the edge of the second installing plate 22.
Relative to prior art, the experiment that the present embodiment is provided is had the advantage that with zebrafish embryo hatching apparatus:
The experiment that the present embodiment is provided when in use, passes through 11 pairs of hatchings of primary heater with zebrafish embryo hatching apparatus Water-bath in case 1 is heated, and the bath temperature in incubator 1 is maintained at a specific temperature, e.g., be may remain in 28.5 DEG C, and good illumination condition is provided for Zebrafish Embryo by the light source of the bottom of the first lid 12.Contained multigroup The culture dish for putting zebrafish embryo is placed in the mounting hole 211 of the first installing plate 21 to be contrasted group and carries out experiment, due to contrast Group is placed on same plane, and it is hereby ensured that the illumination condition that contrast groups receive is identical;Heating water bath is being used to culture dish During, because the diameter ratio of mounting hole 211 and inlet opening 221 is 5:1-4:1, therefore, it can in the unit interval from entering Ratio between the amount of the nutrient solution in water and culture dish inside the turnover of water hole 221 support frame 2 is defined, so as to Influence of the primary heater 11 to the temperature fluctuation of heating water bath to water temperature in support frame 2 is limited, and then makes the water in support frame 2 The fluctuation of heat exchange is smaller between nutrient solution in culture dish, to ensure that the experimental temperature residing for zebrafish embryo is more constant, So that it is guaranteed that between contrast groups illumination and temperature it is constant with uniformly, making experimental result relatively reliable.
Wherein, in order that the water in incubator 1 can preferably pass in and out culture dish heating chamber, so that it is guaranteed that culture dish is heated The water of intracavitary can remain 28.5 DEG C, to meet the incubation temperature of zebrafish embryo, the experiment spot that the present embodiment is provided In the embryo hatching device of horse fish, as shown in figs 6-8, each inlet opening 221 is located at throwing of the mounting hole 211 on the second installing plate 22 Outside the domain of shadow zone.
It is so designed that, it can be ensured that when the culture dish for holding zebrafish embryo is placed in mounting hole 211, culture dish is all the time Do not contacted with inlet opening 221, water is limited in culture dish heating chamber so as to avoid culture dish bottom from blocking inlet opening 221 Situation about being flowed freely between incubator 1, so that water can be freed in and out in culture dish heating chamber with incubator 1, with Ensure that the water temperature in support frame 2 remains 28.5 DEG C, and then may insure that zebrafish embryo can be normally incubated, tie experiment Fruit is more accurate.
Specifically, in order to meet the illumination condition needed for Zebrafish Embryo, zebra is used in the experiment that the present embodiment is provided In the embryo hatching device of fish, as illustrated in figures 4-5, light source can be fluorescent lamp 121, and mounting hole 211 is multiple rows of, and per adjacent two Provided with a fluorescent lamp 121 between row's mounting hole 211;Or, mounting hole 211 is multigroup, and every group includes two row's mounting holes 211, Provided with a fluorescent lamp 121 in the middle of every group of mounting hole 211.Good illumination bar is provided so as to be incubated for zebrafish embryo Part, to ensure that zebrafish embryo be able to can be smoothed out with normal incubation, experiment.
Herein it should be added that, light source can also be a fluorescent lamp 121 for being arranged on the bottom of the first lid 12. Certainly, light source can also be area source, such as LED, so that it is guaranteed that to the illumination condition of each position on the first installing plate 21 It is identical, to ensure the accuracy of contrast and experiment.
When actual production is manufactured, in order to meet the oxygen needed for zebrafish embryo is incubated, to ensure that zebrafish embryo can Normal to be incubated, the experiment that the present embodiment is provided is with zebrafish embryo hatching apparatus, and as shown in Figure 1-2, incubator 1 includes incubating Change portion and air circulation portion, the hatching portion that support frame 2 is placed in incubator 1, the top in air circulation portion offer passage 15.
It is so designed that, outside air can be made to flow into air circulation portion by passage 15, then flowed into from air circulation portion Hatching portion, to realize exchanging for hatching portion and outside air, is incubated demand to oxygen, so that really for meeting zebrafish embryo Protecting experiment can be normally carried out, and cause air not free in and out to avoid the first lid 12 from covering whole incubator 1 and incubate Change case 1, the situation for causing zebrafish embryo not to be incubated normally.
Further, in order to ensure water can the swimmingly free flow between incubator 1 and culture dish heating chamber, with true The water temperature protected in culture dish heating chamber can be in 28.5 DEG C all the time, and then ensure that zebrafish embryo is in isoperibol all the time, The experiment that the present embodiment is provided is with zebrafish embryo hatching apparatus, and as Figure 4-Figure 6, support frame 2 can also include correspondence and set Two supporting legs 24 in the bottom of the second installing plate 22 are put, supporting leg 24 is L-shaped, the top of the L-type vertical portion of supporting leg 24 It is fixedly connected with the bottom of the second installing plate 22.
Supporting leg 24 is designed into l-shaped, it can be ensured that 24 pair of first installing plate 21 of supporting leg, the second installing plate 22 and side plate The stationarity of 23 supports, and the bottom of the second installing plate 22 is correspondingly arranged two supporting legs 24, the second installing plate 22 can be made From the bottom a certain distance of incubator 1 so that water can flow more smooth between incubator 1 and culture dish heating chamber, enter And ensure the water temperature in culture dish heating chamber and kept for 28.5 DEG C.
Wherein, in order to further ensure that water temperature is constant in incubator 1, the constant temperature for making zebrafish embryo be in 28.5 DEG C all the time It is incubated under environment, to ensure the accuracy of experimental result, the experiment that the present embodiment is provided is with zebrafish embryo hatching apparatus, such as Shown in Fig. 1, experiment zebrafish embryo hatching apparatus also includes setting in the storage tank 3 being arranged on outside incubator 1, storage tank 3 Have and be provided with water inlet on the secondary heater 31 for heated water bath, incubator 1, by entering between incubator 1 and storage tank 3 The mouth of a river is connected, and the water inlet in incubator 1 is provided with ball-cock assembly 13.
In being studied in toxicological experiment, zebrafish embryo usually needs to hatch several days to determine toxicant to zebra fish Temperature in embryo hatching influence, and incubator 1 is in 28.5 DEG C all the time, and the moist part in incubator 1 certainly will be caused to steam Hair.In order to ensure the water-bath in incubator 1 maintains certain altitude, the height of water-bath in incubator 1 is set to be consistently higher than in culture dish The position reached the standard grade of liquid, to ensure that preferably with the nutrient solution in culture dish heat exchange can occur for water-bath, make zebra fish embryo Tire is under isoperibol all the time, it is desirable to reach above-mentioned requirements, then needs the make-up water source into incubator 1 in time.But, if Cold water is directly poured into incubator 1 to will be unable to ensure the isoperibol in incubator 1, and is needed artificial frequent in experimentation Water level is observed, it is fairly cumbersome.
Therefore, by being provided with the secondary heater 31 for heated water bath in storage tank 3, it can be ensured that storage tank 3 Interior water is in 28.5 DEG C all the time, and by being provided with water inlet on incubator 1, by entering between incubator 1 and storage tank 3 The mouth of a river is connected, and the water inlet in incubator 1 is provided with ball-cock assembly 13, after the water in incubator 1 vapors away a part, The ball float of ball-cock assembly 13 sinks, ball-cock assembly 13 is opened so that the water in storage tank 3 flows into incubator 1, the water in incubator 1 Rise to after certain altitude, ball float floats, ball-cock assembly 13 is closed, so that the culture dish for holding zebrafish embryo is in one all the time In the water-bath for determining height, it is ensured that zebrafish embryo is in isoperibol.Into incubator 1, make-up water source is more in this way Releive, it can be ensured that water-bath remains steady temperature in incubator 1, and without manually often observing water level, improves experiment people The operating efficiency of member.
Preferably, in order to ensure primary heater 11, secondary heater 31 can be respectively in incubators 1 and storage tank 3 Water-bath heated, and water temperature can maintain to required temperature, the experiment that the present embodiment is provided is hatched with zebrafish embryo In device, as shown in Fig. 1 and Fig. 4-5, primary heater 11 and secondary heater 31 are heating rod or serpentine heating pipe.
Heating rod or serpentine heating pipe in incubator 1 can be placed in the water-bath in air circulation portion, can also be placed in In the water-bath of the lower section of support frame 2, heat is produced by heating rod or serpentine heating pipe, makes heating water bath, maintain a constant temperature Degree, to meet experiment demand.
Specifically, in order to avoid other debris fall into storage tank 3 and influence the development of experiment, the experiment that the present embodiment is provided With in zebrafish embryo hatching apparatus, as Figure 1-3, the top of storage tank 3 is provided with the second lid 33, the second lid 33 By gemel connection between storage tank 3, gemel connection is passed through between the first lid 12 and incubator 1.
By setting the second lid 33 in the top of storage tank 3, when testing, the second lid 33 can be covered in storage tank 3 Top, influences to test to avoid debris from falling into storage tank 3.By between the second lid 33 and storage tank 3 by gemel connection, By gemel connection between first lid 12 and incubator 1, make experimenter is more convenient to open the first lid 12, the second lid 33 Open and close, and then facilitate experimental implementation.
Further, the cleaning for the ease of experimenter to incubator 1 and storage tank 3, the experiment that the present embodiment is provided With in zebrafish embryo hatching apparatus, as Figure 1-3, the bottom of incubator 1 is provided with the first drainpipe 14, the bottom of storage tank 3 It is provided with the second drainpipe 32.
When experimenter cleans to incubator 1 and storage tank 3, plug on the first drainpipe 14 and the need to be only opened Plug on two drainpipes 32, the sewage after cleaning is discharged by the first drainpipe 14, the second drainpipe 32, without will be whole Individual device has been removed, has fallen to remove sewage, so as to reduce the workload of experimenter.
Wherein, in order to further improve lighting effect of the light source to zebrafish embryo, and it is easy to experimenter to zebra fish The observation of water level in embryonic development situation and culture dish heating chamber, the first installing plate 21, the second installing plate 22 and side plate 23 can be with It is made of lucite.
First installing plate 21, the second installing plate 22 and side plate 23 are made of lucite, because lucite has Certain transparency, therefore lighting effect of the light source to zebrafish embryo can be improved, and it is easy to experimenter to add culture dish The observation of hot intracavitary situation.
Certainly, for the ease of the control preferably to the inner light source of incubator 1, incubator 1 should be impermeable using stainless steel etc. Bright material is made, to avoid external light source from impacting experiment.
Finally it should be noted that:Various embodiments above is only limited to illustrate the technical solution of the utility model, rather than to it System;Although the utility model is described in detail with reference to foregoing embodiments, one of ordinary skill in the art should Understand:It can still modify to the technical scheme described in foregoing embodiments, or to which part or whole Technical characteristic carries out equivalent substitution;And these modifications or replacement, the essence of appropriate technical solution is departed from this practicality newly The scope of each embodiment technical scheme of type.

Claims (10)

1. zebrafish embryo hatching apparatus is used in one kind experiment, it is characterised in that including:
Incubator, the incubator inner bottom part is provided with the top of the primary heater for heated water bath, the incubator and set The first lid is equipped with, the first lid bottom is provided with light source;
It is arranged on the support frame in the incubator, support frame as described above includes the first installing plate, parallel with first installing plate The second installing plate set, the edge of first installing plate is connected to form training with the edge of second installing plate by side plate Support and offered on the mounting hole that multiple array distributions are offered on ware heating chamber, first installing plate, second installing plate The diameter ratio of multiple inlet openings, the mounting hole and the inlet opening is 5:1-4:1.
2. zebrafish embryo hatching apparatus is used in experiment according to claim 1, it is characterised in that each inlet opening is located at Outside view field of the mounting hole on second installing plate.
3. zebrafish embryo hatching apparatus is used in experiment according to claim 1, it is characterised in that the light source includes multiple Fluorescent lamp, wherein:
The mounting hole is multiple rows of, and provided with a fluorescent lamp between mounting hole described in per adjacent two row;Or,
The mounting hole is multigroup, and every group is included in the middle of two row's mounting holes, every group of mounting hole provided with a fluorescent lamp.
4. zebrafish embryo hatching apparatus is used in experiment according to claim 1, it is characterised in that the incubator includes incubating Change portion and air circulation portion, the hatching portion that support frame as described above is placed in the incubator, the top in the air circulation portion Offer passage.
5. zebrafish embryo hatching apparatus is used in experiment according to claim 1, it is characterised in that support frame as described above also includes Two supporting legs of the second installing plate bottom are correspondingly arranged at, the supporting leg is L-shaped, and the L-type of the supporting leg is vertical Partial top is fixedly connected with the bottom of second installing plate.
6. zebrafish embryo hatching apparatus is used in experiment according to claim 1, it is characterised in that also described including being arranged on It is provided with the secondary heater for heated water bath, the incubator and sets in storage tank outside incubator, the storage tank There is water inlet, be connected between the incubator and the storage tank by the water inlet, described in the incubator Water inlet is provided with ball-cock assembly.
7. zebrafish embryo hatching apparatus is used in experiment according to claim 6, it is characterised in that the primary heater and The secondary heater is heating rod or serpentine heating pipe.
8. zebrafish embryo hatching apparatus is used in experiment according to claim 6, it is characterised in that the top of the storage tank The second lid is provided with, by gemel connection between second lid and the storage tank, first lid is incubated with described Pass through gemel connection between change case.
9. zebrafish embryo hatching apparatus is used in experiment according to claim 6, it is characterised in that the hatching lower box part is set The first drainpipe is equipped with, the water storage lower box part is provided with the second drainpipe.
10. zebrafish embryo hatching apparatus is used in experiment according to claim 1, it is characterised in that first installing plate, Second installing plate and the side plate are made of lucite.
CN201720103485.6U 2017-01-23 2017-01-23 Zebrafish embryo hatching apparatus is used in experiment Expired - Fee Related CN206423360U (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106719172A (en) * 2017-01-23 2017-05-31 浙江师范大学 Zebrafish embryo hatching apparatus are used in experiment
CN110663645A (en) * 2019-11-21 2020-01-10 湖南富高农业科技开发有限公司 Tadpole is bred and uses hatching apparatus

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106719172A (en) * 2017-01-23 2017-05-31 浙江师范大学 Zebrafish embryo hatching apparatus are used in experiment
CN110663645A (en) * 2019-11-21 2020-01-10 湖南富高农业科技开发有限公司 Tadpole is bred and uses hatching apparatus
CN110663645B (en) * 2019-11-21 2021-10-08 湖南富高农业科技开发有限公司 Tadpole is bred and uses hatching apparatus

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