KR20160036444A - Automatic water bath for sample preprocessor - Google Patents

Automatic water bath for sample preprocessor Download PDF

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Publication number
KR20160036444A
KR20160036444A KR1020140129375A KR20140129375A KR20160036444A KR 20160036444 A KR20160036444 A KR 20160036444A KR 1020140129375 A KR1020140129375 A KR 1020140129375A KR 20140129375 A KR20140129375 A KR 20140129375A KR 20160036444 A KR20160036444 A KR 20160036444A
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South Korea
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water
water tank
temperature
leaching
auxiliary
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KR1020140129375A
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Korean (ko)
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박의광
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박의광
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • C12M1/02Apparatus for enzymology or microbiology with agitation means; with heat exchange means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • C12M1/16Apparatus for enzymology or microbiology containing, or adapted to contain, solid media

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

And provides a pretreatment unit for the animal water bath sample.
The flask is composed of upper and lower two stages by left and right reciprocation according to the driving of the constant temperature bath and shaking motor configured to test the physical properties of the sample and keep the temperature constant by heating the inside of the bath water tank equipped with the hot water heater by the switch operation of the control panel, And an automatic water bath sample preprocessing unit capable of shaking the flask fixed in the rack and automatically stopping the leaching after being shaken under constant temperature by a control unit. The pretreatment device comprises a water tank cover hinged to the upper open side of the cooing bath with a transparent glass attached thereto, and a control panel installed on a front side of the cooing bath. The water bath cover is installed inside and outside of the cooing bath bottom, And a drain valve is provided.
In addition, the constant-temperature shaker is mounted on a bottom of a switch box equipped with a speed controller and a shaking motor installed in a middle portion thereof and is seated in a box seating portion on the upper side of the hot water tank, and flask fixed coil lines on which the flask is seated and fixed are arranged at regular intervals And the flask mounting rack is connected to the shaking motor via a reciprocating motion band and is inserted into the bathtub.
According to the present invention, there is provided a water tank for maintaining a uniform temperature distribution inside a water tank based on a constant-temperature shaker used for testing physical properties of a sample, culture, and soil samples at a constant temperature, The function of the soil sample automatic pretreatment which can pre-treat the soil sample by automatically cooling the constant temperature shaker and the soil sample with cooling water at constant temperature after constant temperature shaking can be conveniently used according to the application as one device. Layer, which has twice the processing power of existing devices.
In addition, since the conventional constant-temperature shaking machine is equipped with a sample and water at room temperature is heated and heated to constantly shake, the water temperature is gradually raised from room temperature to the set temperature until the water is heated. The hot water heated at the set temperature is directly supplied to the lower hot water tank, and since the temperature is maintained by the heating device even after being supplied, the leaching can be performed constantly from the beginning to improve the accuracy of the leaching. If the set temperature is increased by supplying water at room temperature, the seasonal water leaching temperature will vary greatly depending on the season, and the seasonal leaching degree will vary and the accuracy of the experimental results will be lowered.
Also, in case of conventional leaching with constant temperature shaking, water is used for cooling with cold water to stop the leaching, and the cooling rate is different because the difference of water temperature (below constant temperature) is large depending on the season. Because the constant temperature is high in summer, the time to reach a certain temperature takes longer than winter, and since the constant temperature is low in winter, the time to reach a certain temperature takes a short time. Since the results of the test are also different and the cooling time and the cooling method for stopping the leaching are not specified, it is possible to proceed by manual operation, resulting in errors depending on the operator and the environment. Therefore, the degree of leaching using the automatic pre- The accuracy of the experiment can be increased by constantly standardizing it.
SUMMARY OF THE INVENTION The present invention has been made in view of the above circumstances, and it is an object of the present invention to provide a device for automatically operating a pre-treatment process based on a constant temperature shaker, And a sample pretreatment unit capable of standardizing the degree of leaching due to shaking, hot water, and cooling.

Figure pat00001

Description

Automatic water bath for sample preprocessor

The technical idea of the present invention relates to a water boiler, a constant temperature shaker used for testing the properties of a sample under constant temperature, a sample incubator for soil chemical analysis, and the like.

As a background of the present invention, there is a hot water bath of a water bath, which is composed of a warming device, a reciprocating shaking function, a temperature control sensor and a timer.

The present invention relates to a pre-treatment apparatus for an automatic water-boiling-water sample, in which a function of warming-up, cooling and leaching-out is further added to a boiler having an existing water-boiling function, The present invention relates to a pretreatment apparatus for automatically driving a pretreatment such as a cooling process after shaking in a water tank on the basis of a constant temperature bath used for physical property testing and germ culture.

In general, the water pre-treatment system is used for testing and culturing bacteria at constant temperature. It is mainly used for experimental equipment such as experiment tube, beaker or flask. In order to prevent the keeping and evaporation of hot water in the bath, the water tank and the cover are made of stainless steel Steel plate or the same or similar material.

In addition, the constant temperature shaker is widely used for germ culture and soil sample pretreatment by shaking in a water tank, and is configured to maintain a constant temperature within a set temperature of ± 1 ° C.

On the other hand, in the laboratory, in the case of using the water tank, there is a case where the sample is to be cooled after the constant temperature shaking in the case of the pretreatment of the soil sample. In addition, not only all of the existing processes are performed manually, The pretreatment effect of

SUMMARY OF THE INVENTION The present invention has been made in view of the above circumstances, and it is an object of the present invention to provide a device for automatically operating a pre-treatment process based on a constant temperature shaker, And a sample pretreatment unit capable of standardizing the degree of leaching due to shaking, hot water, and cooling.

The problems to be solved by the present invention are not limited to the above-mentioned problems, and other matters not mentioned can be clearly understood by those skilled in the art from the following description.

According to an aspect of the present invention, there is provided an automatic water heater sample preprocessor comprising: a cold / hot water tank for supplying cold / hot water for leaching and stopping a sample; The inside of the installed bath water tank is kept warm and kept at a constant temperature, so that it can be tested for the physical properties of the sample and incubated in a flask equipped with a thermostatic stirrer and a shaking motor. The flask can be shaken And an automatic water bath sample preprocessing unit configured to automatically stop the leaching after being cooled by the constant temperature shaking by the control unit.

The automatic pretreatment sample preprocessing machine includes a water tank cover hinged with a transparent glass on the upper open side of the cooing bath, hinged to the inside of the water bath cover, a control panel installed on the front side of the cooing bath, And a temperature sensor and a drain valve are installed inside and outside.

In addition, the constant-temperature shaker has a switch box bottom portion with a speed controller and a shaking motor selectively installed at the middle portion thereof, and is seated in a box seating portion on the upper side of the hot water tank. The flask fixed- And the arranged flask mounting rack is connected to the shaking motor through a reciprocating motion band and inserted into the bathtub.

In addition, the flask mounting rack is selectively removable, and the upper and lower ends of the flask are selectively formed of two layers or a single layer.

The automatic pretreatment sample preprocessor includes a water tank, a cooling device, and a heating device, each of which is capable of maintaining cold water and hot water at all times in the lower part of the constant temperature water tank, a water supply system for supplying hot water for leaching of the sample, A level sensor capable of maintaining the water level and a water drain for draining the used hot water to the existing hot water tank, a water supply system for supplying cold water for stopping the leaching of the sample, a level center for maintaining a certain water level, And a drain port capable of distributing the cold water to the existing cold water tank.

The control panel for the cold / hot water supply / drainage water is displayed on one side of the front part.

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, preferred embodiments of an automatic water heater sample pretreatment apparatus according to the present invention will be described in detail with reference to the accompanying drawings.

The details of other embodiments are included in the detailed description and drawings.

As described above, according to the automatic water heater sample preprocessing machine of the present invention, it is possible to provide a water tank stirrer for uniformly maintaining the temperature distribution inside the water tank based on the constant temperature shaker used for the physical property test, the bacterial culture and the soil sample pre- The function of the soil sample automatic pretreatment which can pre-treat the soil sample by automatically cooling the constant temperature shaker and the soil sample used in the shaking in the water tank with the cooling water of constant temperature after the constant temperature shaking is used as one device It can be used conveniently and it has two layers of upper and lower layers and has twice the processing capacity than the existing one.

In addition, since the conventional constant-temperature shaking machine is equipped with a sample and water at room temperature is heated and heated to constantly shake, the water temperature is gradually raised from room temperature to the set temperature until the water is heated. The hot water heated to the set temperature is directly supplied to the hot water tank in the lower part. Since the temperature is maintained by the heating device even after being supplied, the leaching can be performed constantly from the beginning to improve the accuracy of leaching.

In addition, if the set temperature is increased by supplying water at room temperature as in the conventional case, the seasonal variation in the water supply temperature will cause a difference in the water supply temperature depending on the season.

Also, in case of conventional leaching with constant temperature shaking, water is used for cooling with cold water to stop the leaching, and the cooling rate is different because the difference of water temperature (below constant temperature) is large depending on the season. Because the constant temperature is high in summer, the time to reach a certain temperature takes longer than winter, and since the constant temperature is low in winter, the time to reach a certain temperature takes a short time. The test results also differ.

In addition, since the cooling time and the cooling method for stopping the leaching are not specified, errors may occur depending on the operator and the environment due to manual operation. Therefore, by standardizing the degree of leaching constantly by using the automatic pre- Can be increased.

It should be noted that the above-described embodiment is only one preferred embodiment for carrying out the multi-use water heater of the present invention, and the present invention is not limited to the above-described embodiment, It is to be understood that various changes may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

1 to 3 show the front and side structures of the pretreater of the present invention. Fig. 5 is a graph showing the relationship between the amount of bacteria A constant-temperature shaker 40 used for sample preprocessing, and an operation structure of the automatic sample preprocessor.
As shown in FIGS. 1 to 3, the water bath in the present invention has a basin water tank in which a bath water heater 15 is installed inside and a control panel 14 in which a number of function switches are arranged outside, A hot water tank 48 and a cold water tank 49. The hot water tank 48 includes a heating device 54 for raising the temperature to maintain a constant temperature, a water supply device 51 for supplying heated hot water, A cooling device 55 for cooling the temperature of the cooling water for stopping the leaching to a low temperature, a water supply device 50 for supplying the cooled cold water, A water feed motor 57 and a drain device 53 for discharging used cold water and an automatic water heater sample preprocessor 10 having a control box 14 capable of automatically controlling them.
A flask mounting rack 41 which is connected to a speed-controlled shaking motor 46 is fixed in the bath water tank 12 as a basic constitution and is supplied to the bath water tank 12 shaken by the driving of the shaking motor 46 A constant-temperature shaker 40 is constructed by flask 47. As shown in FIG. 4, the constant temperature water heater 10 is hinged to a water tank cover to which the inside of the water bath tank 12 can be confirmed by attaching a transparent glass to the open upper side of the water bath 12, A control panel 14 having a plurality of function switches arranged at one side of the front of the hot water tank 12 is provided at the front side of the hot water tank 12, And the water tank cover 11 are made of a stainless steel plate or a material of the same type or higher so that they can be used semi-permanently without corrosion.
A box seating part for seating and fixing the switch box 43 of the constant temperature stove 10 is formed at one side of the upper part of the stagnant water tank 12 by the side of the water tank cover 11. The stagnant water tank 12, A temperature sensor (17) for detecting the level of water and a drain valve (16) for draining the excess water used are installed on the inside and the outside of the bottom part, and the outside of the bottom of the bath water tank (12) 18, the inside of the water bath 12 is filled with a heat insulating material.
It is also preferable that a warning sound of a buzzer (not shown) and a power source of the hot water heater 15 are cut off when the water level in the hot water tank 12 is lowered, thereby preventing an overheating accident.
As shown in FIG. 5, the constant-temperature shaker 40 includes a flask mounting rack (not shown), which is a basic constitution of the present invention, 41 are inserted and seated into the water bath 12 so that the contents of the flask fixing device 44 are shaken while the flask mounting rack 41 is vibrated at a constant speed by the driving of the shaking motor 46 And the water temperature in the flask mounting rack 41, which is a shaking water tank, can be kept constant at a set temperature + 1 ° C through a temperature controller (not shown).
The flask mounting rack 41 is connected to the bottom of a switch box 43 with a speed control switch via a shaking motor 46 and a reciprocating moving base 45. The switch box 43 is connected to a hot water tank 12, the shaking motor 46 below the shaking motor 46 is inserted and seated in the box seating part at the rear of the control panel 14, and the flask mounting rack 41 May be shaken within the water bath 12 at a constant speed.
In addition, the automatic pre-treatment machine for pre-heated water samples the water supplied by the heating device 54 provided inside the hot water tank 48 for hot water supply, the hot water heated to the set temperature is stored in the hot water tank, The hot water is supplied into the hot water tank 12 by the hot water supply system 51 with the hot water supply motor 56 and discharged to the hot water drain port 52 after the constant temperature shaking for a predetermined time by the set control system.
The cold water cooled to the set temperature by the cooling device 55 in the cold water tank 49 is stored in the cold water tank 49 which is kept warm. After the warm water is discharged by the control system described above, (57) is supplied into the hot water tank (12) by the provided cold water supply system (50), and the sample is cooled by shaking to complete the stop of the leaching.
Also, the cooling water used after the shaking for a set time is discharged by the cooling water drain port (53).

BRIEF DESCRIPTION OF THE DRAWINGS The advantages and features of the present invention, and the manner of achieving them, will be apparent from and elucidated with reference to the embodiments described hereinafter in conjunction with the accompanying drawings. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the concept of the invention to those skilled in the art. It is intended that the scope of the invention be defined by the claims and the equivalents thereof. The dimensions and relative sizes of layers and regions in the figures may be exaggerated for clarity of illustration. Like reference numerals refer to like elements throughout the specification.

The embodiments described herein will be described with reference to cross-sectional views, plan views, and block diagrams, which are ideal schematics of the present invention. Thus, the shape of the illustrations may be modified by manufacturing techniques and / or tolerances. Accordingly, the embodiments of the present invention are not limited to the specific forms shown, but also include changes in the shapes that are generated according to the manufacturing process. Thus, the regions illustrated in the figures have schematic attributes, and the shapes of the regions illustrated in the figures are intended to illustrate specific types of regions of the elements and are not intended to limit the scope of the invention.

In the drawings, the thicknesses of layers and regions are exaggerated for clarity. Also, when a layer is referred to as being "on" another layer or substrate, it may be formed directly on another layer or substrate, or a third layer may be interposed therebetween. Like numbers refer to like elements throughout the specification.

Terms such as top, bottom, top, bottom, or top, bottom, etc. are used to distinguish relative positions in components. For example, in the case of naming the upper part of the drawing as upper part and the lower part as lower part in the drawings for convenience, the upper part may be named lower part and the lower part may be named upper part without departing from the scope of right of the present invention .

In addition, terms such as "upper," "middle," and " lower "are used to distinguish relative positions between components, and the technical idea of the present invention is not limited by these terms. Accordingly, terms such as "upper," "intermediate," and " lower "and the like are replaced by terms such as" first ", " second " ≪ / RTI >

The terms "first "," second ", and the like can be used to describe various components, but the components are not limited by the terms. The terms are used only for the purpose of distinguishing one component from another. For example, "first component" may be named "second component" without departing from the scope of the present invention.

The terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the scope of the present invention.

The singular expressions include plural expressions unless the context clearly dictates otherwise. In this application, the terms "comprises", "having", and the like are used to specify that a feature, a number, a step, an operation, an element, a part or a combination thereof is described in the specification, But do not preclude the presence or addition of one or more other features, integers, steps, operations, components, parts, or combinations thereof.

Unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in commonly used dictionaries are to be interpreted as having a meaning consistent with the meaning in the context of the relevant art and are to be construed as ideal or overly formal in meaning unless explicitly defined in the present application Do not.

While the present invention has been described in connection with what is presently considered to be practical exemplary embodiments, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, It will be understood. It is therefore to be understood that the above-described embodiments are illustrative and not restrictive in every respect.

11: Tank cover
12: Bath water tank
14: Control device
15: Heated bath heater
16: drain valve
17: Temperature sensor
18: Support
19: Tank cover handle
40: constant temperature shaker
41: Flask mounting rack
43: Can position box
44: Flask fixing device
45: Round trip
46: Shaking motor
47: Flask
49: cold water tank
50: cold water supply system
51: Hot water supply system
52: Hot water drain
53: cold water drain
54: Heating device
55: cooling device
56: Hot Water Supply Motor
57: cold water feed motor

Claims (10)

Animal water bath pretreatment;
A first auxiliary device for automatically supplying and draining hot water from the animal animal water pre-processor, a second auxiliary device for automatically supplying and draining cold water, a flask taking-out brakes for performing a constant temperature stirring function, And a control box equipped with a control device capable of being pre-processed into a single unit.
The apparatus according to claim 1, further comprising: an auxiliary heating device for heating the liquid in the first auxiliary water tank; And a first water supply device connected to the main water tank and the first auxiliary water tank to supply the liquid in the first auxiliary water tank into the main water tank. The apparatus according to claim 1, further comprising: a cooling device for cooling the liquid in the second auxiliary water tank; And a second water supply device connected to said main water tank and said second auxiliary water tank for supplying liquid in said second auxiliary water tank into said main bath. The water treatment system according to claim 2, wherein the first water supply device includes a first water supply motor, and a first water supply pipe between the main water tank and the first water supply motor, and between the first water supply motor and the first water supply motor,
The shaker according to claim 3, wherein the second water supply device includes a second water supply motor, and a second water supply pipe between the main water tank and the second water supply motor, and between the second water supply tank and the second water supply motor.
The apparatus according to claim 2 or 3, wherein the first water supply device and the second water supply device further comprise a discharge device for discharging the liquid in the main water tank to the first auxiliary water tank or the second auxiliary water tank . 6. The apparatus according to claim 5, wherein the first water supply device is a valve device connected to a discharge part of the main water tank, a first discharge pipe between the valve device and the first auxiliary water tank, and a second discharge pipe between the valve device and the second auxiliary water tank And a second discharge piping. 6. The apparatus according to claim 5, wherein the discharge device is a first discharge pipe between the main water tank and the first auxiliary water tank, a first valve device connected to the first discharge pipe, a second valve device connected between the main water tank and the second auxiliary water tank, A second discharge pipe and a second valve device connected to the second discharge pipe. The control panel according to claim 1, comprising a control system capable of automatically setting the supply and exhaust of hot water, the supply and exhaust of cold water, the temperature control of cold and hot water, the RPM control of the shaking motor, the timer and the series of processes. The flask mounting rack according to claim 1, wherein the flask lifting bracket is composed of two upper and lower layers capable of being detached and attached, and can be selectively driven as a single layer or two layers. The lid as claimed in claim 1, wherein the hinge-coupled top cover is provided with a transparent glass plate at a predetermined portion thereof.
KR1020140129375A 2014-09-25 2014-09-25 Automatic water bath for sample preprocessor KR20160036444A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108029680A (en) * 2018-02-10 2018-05-15 青岛瑞顺通工贸有限公司 The thawing apparatus of animal frozen semen
CN112708549A (en) * 2020-12-10 2021-04-27 贵阳学院 Microbial cultivation device for soil heavy metal pollution restoration
CN114591828A (en) * 2022-01-21 2022-06-07 长春中医药大学 Temperature regulation and control device for cell culture
CN115855560A (en) * 2022-10-24 2023-03-28 北京林业大学 Robot is taken in integration of submarine soil sample water sample
CN116371501A (en) * 2023-04-27 2023-07-04 杭州科玲生物科技有限公司 Constant-temperature water bath box

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108029680A (en) * 2018-02-10 2018-05-15 青岛瑞顺通工贸有限公司 The thawing apparatus of animal frozen semen
CN112708549A (en) * 2020-12-10 2021-04-27 贵阳学院 Microbial cultivation device for soil heavy metal pollution restoration
CN114591828A (en) * 2022-01-21 2022-06-07 长春中医药大学 Temperature regulation and control device for cell culture
CN114591828B (en) * 2022-01-21 2024-04-26 长春中医药大学 Temperature regulation and control device for cell culture
CN115855560A (en) * 2022-10-24 2023-03-28 北京林业大学 Robot is taken in integration of submarine soil sample water sample
CN115855560B (en) * 2022-10-24 2023-08-04 北京林业大学 Robot is adopted in soil sample water sample integration of water bottom
CN116371501A (en) * 2023-04-27 2023-07-04 杭州科玲生物科技有限公司 Constant-temperature water bath box
CN116371501B (en) * 2023-04-27 2023-12-15 杭州科玲生物科技有限公司 Constant-temperature water bath box

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