CN114591828A - Temperature regulation and control device for cell culture - Google Patents
Temperature regulation and control device for cell culture Download PDFInfo
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- CN114591828A CN114591828A CN202210074245.3A CN202210074245A CN114591828A CN 114591828 A CN114591828 A CN 114591828A CN 202210074245 A CN202210074245 A CN 202210074245A CN 114591828 A CN114591828 A CN 114591828A
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- 238000004113 cell culture Methods 0.000 title claims abstract description 26
- 230000007246 mechanism Effects 0.000 claims abstract description 97
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 60
- 230000001105 regulatory effect Effects 0.000 claims abstract description 4
- 239000003153 chemical reaction reagent Substances 0.000 claims description 35
- 230000000670 limiting effect Effects 0.000 claims description 18
- 230000001276 controlling effect Effects 0.000 claims description 9
- 230000001360 synchronised effect Effects 0.000 claims description 5
- 230000010355 oscillation Effects 0.000 abstract description 31
- 238000000034 method Methods 0.000 abstract description 8
- 230000008569 process Effects 0.000 abstract description 5
- 239000000243 solution Substances 0.000 description 15
- 230000009471 action Effects 0.000 description 14
- 230000000694 effects Effects 0.000 description 10
- 230000005540 biological transmission Effects 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 230000007306 turnover Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000010030 laminating Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/25—Greenhouse technology, e.g. cooling systems therefor
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Abstract
The invention discloses a temperature regulating device for cell culture, which belongs to the technical field of laboratory equipment and comprises a water bath tank, a rotating opening is formed in the bottom side of the water bath tank, a rotating plate is rotatably connected to the outer side of the rotating opening, a rotating frame is fixedly connected to the outer side of the rotating plate, the two sides of the rotating frame are respectively connected with an opening and closing frame in a sliding way, the far end of the opening and closing frame is rotationally connected with a supporting plate, the inside of the rotating frame is provided with an opening and closing mechanism which controls the two opening and closing frames to synchronously approach and separate, the inner side of the water bath box is provided with an auxiliary mechanism which is matched with the opening and closing mechanism to control the two supporting plates to synchronously and reversely move, the upper side of each supporting plate is provided with a plurality of working mechanisms, can satisfy multiple needs, and in the oscillation process two backup pads motion synchronization and opposite direction, avoid causing the effort to produce extra oscillation to whole mechanism, guarantee water bath quality.
Description
Technical Field
The invention belongs to the technical field of laboratory equipment, and particularly relates to a temperature regulating device for cell culture.
Background
The cell culture technology is used for simulating the internal environment of a construct in vitro, and comprises sterile conditions, proper temperature, pH value and certain nutritional conditions, so that cells can survive, grow and reproduce in the in vitro environment and maintain the main structure and functions.
Chinese patent document CN112206741A discloses, including disclosing small-size accuse temperature shaking table, including the shell, seted up the cavity on the shell, four corners all are fixed with solid fixed ring at the bottom of the cavity of cavity, and it has colluded the spring to hang on the solid fixed ring, and the top of shell articulates there is the top cap, installs wabbler mechanism in the shell, and wabbler mechanism includes rotating motor, rocking plate and incubator. According to the invention, the turntable and the rocking plate are connected through the arranged eccentric shaft, and the four corners of the rocking plate are hooked by the hook springs, so that the turntable can drive the rocking plate to horizontally swing through the eccentric shaft when rotating, meanwhile, the swing motor can drive the rotating plate and the shifting column to stir the swinging plate through the arranged swing motor, the swinging plate can longitudinally swing at the top of the rocking plate, and thus, the incubator can simultaneously perform horizontal swinging and longitudinal swinging actions, and the test tube, the conical flask and the incubation box can be simultaneously placed in the incubator through the bottom butt strap, the conical flask support, the top butt strap and the test tube clamp rope which are arranged in the incubator.
Present temperature control shaking table cooperation water bath is rocking the in-process, often only can carry out the unilateral reciprocating motion during the oscillation of temperature control shaking table, lack the variety, can't satisfy different oscillation needs, can produce the oscillation to equipment itself at the oscillation in-process simultaneously, the oscillation of equipment itself also can influence inside warm water oscillation condition and influence the water bath quality, and when reagent bottle removed to one end when the warm water collided the inner wall by the recoil, make the warm water touch the reagent bottleneck very easily and cause the pollution.
In view of the above-mentioned disadvantages, the present invention provides a temperature control device for cell culture.
Disclosure of Invention
The invention aims to: in order to solve the problems that the existing water bath temperature control shaking table has single function in the using process and water is easy to splash to a bottle mouth to cause infection, the temperature control device for cell culture is provided.
In order to achieve the purpose, the invention adopts the following technical scheme:
a temperature regulating device for cell culture comprises a water bath box, wherein a rotating opening is formed in the bottom side of the water bath box, a rotating plate is rotatably connected to the outer side of the rotating opening, a rotating frame is fixedly connected to the outer side of the rotating plate, opening and closing frames are respectively connected to two sides of the rotating frame in a sliding mode, a supporting plate is rotatably connected to the far end of each opening and closing frame, an opening and closing mechanism for controlling the two opening and closing frames to synchronously approach and separate is arranged inside each rotating frame, an auxiliary mechanism matched with the opening and closing mechanisms to control the two supporting plates to synchronously move in opposite directions is arranged on the inner side of the water bath box, and a plurality of working mechanisms are arranged on the upper sides of the supporting plates;
the operating mechanism comprises two electric telescopic rods and an undercarriage, wherein one end of each electric telescopic rod is fixedly connected to the upper side of the support plate, the two ends of the undercarriage are respectively connected to the far ends of the two electric telescopic rods in a rotating mode, a plurality of uniformly distributed placing grooves are formed in one side of the undercarriage, a placing mechanism is arranged on the outer side of each placing groove, a control mechanism for controlling the plurality of undercarriage to synchronously rotate is arranged on the upper side of the support plate, and a plurality of uniformly distributed primary constant temperature grooves are formed in the support plate.
As a further description of the above technical solution:
the opening and closing mechanism comprises an opening and closing gear and two limiting gears, the opening and closing gear is rotatably connected inside the rotating frame, an opening and closing motor is fixedly connected to the lower side of the rotating plate and located inside the rotating opening, the output end of the opening and closing motor is fixedly connected with one side of the opening and closing gear, the two limiting gears are rotatably connected to the two ends of the rotating frame respectively, opening and closing gear structures are arranged on the inner sides of the opening and closing frame respectively, and the opening and closing gear structures are meshed with the opening and closing gear and the limiting gears respectively.
As a further description of the above technical solution:
the auxiliary mechanism comprises two auxiliary frames and two auxiliary screw rods, the two auxiliary grooves are formed in the two sides of the water bath box respectively, the two ends of each auxiliary screw rod are connected to the two ends of the two auxiliary grooves respectively in a rotating mode, the two ends of each auxiliary screw rod are connected to the two ends of the two auxiliary grooves respectively, the two outer sides of the auxiliary frames are connected to the two auxiliary screw rods in a threaded mode respectively, the two sides of the auxiliary frames are connected to the two supporting plates respectively in a sliding mode, and the two outer sides of the water bath box are provided with driving mechanisms for controlling the two auxiliary screw rods to rotate synchronously.
As a further description of the above technical solution:
the driving mechanism comprises a driving cylinder, the two ends of the driving cylinder are respectively connected to the two ends of the water bath box in a rotating mode, the outer side of the water bath box is connected with two driven bevel gears in a rotating mode, the two driven bevel gears are respectively connected and fixed with one ends of the two auxiliary screw rods, the two ends of the driving cylinder are respectively fixedly connected with a driving bevel gear meshed with the two driven bevel gears, a driving motor is fixedly connected to the outer side of the water bath box, and the output end of the driving motor is fixedly connected with one end of the driving cylinder.
As a further description of the above technical solution:
and a grabbing rod is arranged on the upper side of the undercarriage.
As a further description of the above technical solution:
the placing mechanism comprises a placing rack, the two ends of the placing rack are fixedly connected to the two ends of the placing groove respectively, a placing plate is fixedly connected to the lower side of the placing rack, a limiting groove is formed in the upper side of the placing plate, and a clamping mechanism which is matched with the placing plate to clamp and fix the reagent bottles is arranged on the outer side of the placing groove.
As a further description of the above technical solution:
the clamping mechanism comprises a clamping arc plate, one end of the clamping arc plate is rotatably connected to one end of the placing groove, a clamping hole is formed in one side of the placing groove, a clamping spring is fixedly connected to the inside of the clamping hole, the far end of the clamping spring is fixedly connected to the inner side of the clamping arc plate, and a clamping assembly is arranged at the far end of the placing groove.
As a further description of the above technical solution:
clamping component is including pressing the sloping block, standing groove one end is opened has and is pressed the groove, press sloping block sliding connection in pressing the inslot portion, press groove one end fixedly connected with and press the spring, press spring distal end fixed connection in pressing sloping block one end.
As a further description of the above technical solution:
and a plurality of secondary constant temperature grooves which are uniformly distributed are formed in the inner side of the limiting groove.
As a further description of the above technical solution:
control mechanism includes the control frame, control frame one side is rotated and is connected with a plurality of synchronizing frame, the inboard rotation of control frame is connected with a plurality of synchronizing gear, and is a plurality of synchronizing gear respectively with a plurality of synchronizing frame fixed connection, control frame one side is equipped with electronic slide rail, electronic slide rail removes a plurality of control gear mechanism of end fixedly connected with, and is a plurality of control gear mechanism meshes with a plurality of synchronizing gear respectively mutually, the inboard size of synchronizing frame laminates with undercarriage one end mutually.
In summary, due to the adoption of the technical scheme, the invention has the beneficial effects that:
1. according to the invention, the water bath box is arranged, the supporting plate is installed in the water bath box through the opening and closing mechanism, the auxiliary mechanism is matched to limit the supporting plate, the placing mechanism is used for clamping a reagent bottle containing cells and culture solution in the undercarriage, the operation can be completed only by starting the opening and closing mechanism when the reagent bottle oscillates in one-way reciprocating motion, the driving motor is started to drive the auxiliary screw rods at two ends to synchronously rotate, the auxiliary frames at two ends can be controlled to synchronously move in the opposite directions, the supporting plate can be controlled to complete multi-directional motion under the action of the opening and closing mechanism, so that the oscillating directions of the reciprocating motion are various, various requirements can be met, the two supporting plates are synchronously moved and have opposite directions in the oscillating process, additional oscillation caused by acting force on the integral mechanism is avoided, and the water bath quality is ensured.
2. According to the invention, the control mechanism is arranged above the supporting plate, and in the process of unidirectional reciprocating motion, the electric slide rail is controlled to drive the synchronous frame to rotate when the reagent bottle moves to two ends, so that the undercarriage is driven to turn over at a certain angle, and on the premise of ensuring the oscillation efficiency, water is prevented from backflushing to the outer side of the reagent bottle under the impact of the two ends to pollute a reagent bottle opening, and the safety is improved.
3. According to the invention, the placing mechanism is arranged on one side of the undercarriage, the reagent bottle is placed in the limiting groove above the placing plate, the placing stability of the reagent bottle is improved under the action of the limiting groove, and the primary constant temperature groove and the secondary constant temperature groove are respectively formed in the lower sides of the supporting plate and the placing plate, so that warm water can completely contact the outer side of the reagent bottle, the water bath quality can be kept on the premise of ensuring normal operation, the reagent bottle can be conveniently clamped and fixed under the action of the clamping mechanism and the clamping assembly, and the operation is simple and convenient.
Drawings
FIG. 1 is a schematic view of the overall structure of a temperature control device for cell culture according to the present invention;
FIG. 2 is a schematic structural view of a bottom mechanism of a water bath tank of the temperature control device for cell culture according to the present invention;
FIG. 3 is a schematic view of the installation structure of the water bath box and the opening and closing mechanism of the temperature control device for cell culture according to the present invention;
FIG. 4 is a schematic structural diagram of an opening and closing mechanism of a temperature control device for cell culture according to the present invention;
FIG. 5 is a schematic view of the internal structure of a temperature control device landing gear for cell culture according to the present invention;
FIG. 6 is an enlarged schematic view of region A of a temperature control device for cell culture according to the present invention;
FIG. 7 is an enlarged view of the region B of the temperature control device for cell culture according to the present invention.
Illustration of the drawings:
1. a water bath tank; 101. a rotation port; 102. a rotating plate; 103. a rotating frame; 104. opening and closing frames; 105. a support plate; 106. a primary thermostatic bath; 107. a grabbing rod; 2. an opening and closing mechanism; 201. an opening and closing gear; 202. a limit gear; 203. an opening and closing motor; 204. an opening and closing gear structure; 3. an auxiliary mechanism; 301. an auxiliary frame; 302. an auxiliary screw rod; 303. an auxiliary groove; 304. a drive mechanism; 305. a drive cylinder; 306. a driven bevel gear; 307. a drive bevel gear; 308. a drive motor; 4. a working mechanism; 401. an electric telescopic rod; 402. a landing gear; 403. a placement groove; 5. a placement mechanism; 501. placing a rack; 502. placing the plate; 503. a limiting groove; 504. a clamping mechanism; 505. clamping the arc plate; 506. a clamping hole; 507. a clamping spring; 508. a clamping assembly; 509. pressing the inclined block; 510. pressing the groove; 511. a pressing spring; 512. a secondary thermostatic bath; 6. a control mechanism; 601. a control frame; 602. a synchronous frame; 603. a synchronizing gear; 604. an electric slide rail; 605. the gear mechanism is controlled.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-7, the present invention provides a technical solution: a temperature control device for cell culture comprises a water bath tank 1, wherein a rotary opening 101 is formed in the bottom side of the water bath tank 1, a rotary plate 102 is rotatably connected to the outer side of the rotary opening 101, a rotary frame 103 is fixedly connected to the outer side of the rotary plate 102, opening and closing frames 104 are respectively connected to two sides of the rotary frame 103 in a sliding mode, a support plate 105 is rotatably connected to the far end of each opening and closing frame 104, an opening and closing mechanism 2 for controlling the two opening and closing frames 104 to synchronously approach and separate is arranged inside the rotary frame 103, an auxiliary mechanism 3 for controlling the two support plates 105 to synchronously and reversely move in a matching mode with the opening and closing mechanism 2 is arranged on the inner side of the water bath tank 1, and a plurality of working mechanisms 4 are arranged on the upper side of each support plate 105;
the operating mechanism 4 comprises two electric telescopic rods 401 and an undercarriage 402, two one ends of the electric telescopic rods 401 are fixedly connected to the upper side of the supporting plate 105, two ends of the undercarriage 402 are respectively connected to the far ends of the two electric telescopic rods 401 in a rotating mode, a plurality of uniformly distributed placing grooves 403 are formed in one side of the undercarriage 402, a placing mechanism 5 is arranged on the outer side of each placing groove 403, a control mechanism 6 for controlling the plurality of undercarriage 402 to rotate synchronously is arranged on the upper side of the supporting plate 105, and a plurality of uniformly distributed primary constant temperature grooves 106 are formed in the supporting plate 105.
The implementation mode is specifically as follows: place water bath 1 in the laboratory required position, with warm water injection water bath 1 inside, the cell that will accomplish the intensification and culture solution injection reagent bottle inside, be fixed in placing mechanism 5 inside and control electric telescopic handle 401 completion position control, only start opening and shutting mechanism 2 when needing the unilateral reciprocating oscillation and can carry out the unilateral reciprocating oscillation to the reagent bottle, and the certain angle of upset of supplementary reagent bottle self-adaptation when removing to both ends under control mechanism 6's effect, avoid appearing the pollution phenomenon, start complementary unit 3 once more when needing other oscillation directions, can carry out multi-directional reciprocating motion under complementary unit 3 cooperation opening and shutting mechanism 2's effect.
The opening and closing mechanism 2 comprises an opening and closing gear 201 and two limiting gears 202, the opening and closing gear 201 is rotatably connected inside the rotating frame 103, an opening and closing motor 203 is fixedly connected to the lower side of the rotating plate 102, the opening and closing motor 203 is located inside the rotating opening 101, the output end of the opening and closing motor 203 is fixedly connected with one side of the opening and closing gear 201, the two limiting gears 202 are respectively rotatably connected to two ends of the rotating frame 103, opening and closing gear structures 204 are respectively arranged on the inner sides of the opening and closing frames 104, and the opening and closing gear structures 204 are respectively meshed with the opening and closing gear 201 and the limiting gears 202.
The implementation mode is specifically as follows: start motor 203 that opens and shuts and drive gear 201 that opens and shuts and rotate, cooperation swivel mount 103 and two limit gear 202 are to two spacing actions that open and shut the frame 104 under, through the meshing effect of opening and shutting gear 201 and opening and shutting gear structure 204, can control two backup pads 105 and carry out the oscillation operation that is close to in step and accomplishes reciprocating motion with the separation, two backup pads 105 opposite direction of motion at this in-process, avoid causing the oscillation and influence the oscillation condition of warm water to holistic mechanism, and then influence the water bath effect.
The auxiliary mechanism 3 comprises two auxiliary frames 301 and two auxiliary screw rods 302, two auxiliary grooves 303 are respectively formed in two sides of the water bath tank 1, two ends of the two auxiliary screw rods 302 are respectively connected to two ends of the two auxiliary grooves 303 in a rotating manner, the outer sides of the two auxiliary frames 301 are respectively connected to the inner parts of the two auxiliary grooves 303 in a sliding manner, the outer sides of the two auxiliary frames 301 are respectively connected with the two auxiliary screw rods 302 in a threaded manner, two sides of the auxiliary frames 301 are respectively connected to two sides of the two support plates 105 in a sliding manner, a driving mechanism 304 for controlling the two auxiliary screw rods 302 to synchronously rotate is arranged on the outer side of the water bath tank 1, the driving mechanism 304 comprises a driving cylinder 305, two ends of the driving cylinder 305 are respectively connected to two ends of the water bath tank 1 in a rotating manner, two driven bevel gears 306 are rotatably connected to the outer side of the water bath tank 1, and the two driven bevel gears 306 are respectively connected and fixed to one end of the two auxiliary screw rods 302, two ends of the driving cylinder 305 are fixedly connected with a driving bevel gear 307 engaged with two driven bevel gears 306 respectively, the outer side of the water bath tank 1 is fixedly connected with a driving motor 308, and the output end of the driving motor 308 is fixedly connected with one end of the driving cylinder 305.
The implementation mode is specifically as follows: when the auxiliary frames 301 are fixed at two ends of the water bath tank 1, the auxiliary frames 301 can be matched with the opening and closing mechanism 2 to complete the unidirectional reciprocating motion of the supporting plate 105 under the limiting action of the auxiliary frames 301 on the supporting plate 105, when the multidirectional reciprocating motion oscillation is required, the driving motor 308 is controlled to drive the driving cylinder 305 to rotate, the auxiliary screw rod 302 is controlled to synchronously rotate under the meshing transmission action of the driving bevel gear 307 and the driven bevel gear 306, the two auxiliary frames 301 are driven to synchronously move in opposite directions, and the supporting plate 105 can be controlled to complete the multidirectional reciprocating oscillating motion by being matched with the opening and closing mechanism 2.
The placing mechanism 5 comprises a placing rack 501, two ends of the placing rack 501 are fixedly connected to two ends of a placing groove 403 respectively, a placing plate 502 is fixedly connected to the lower side of the placing rack 501, a limiting groove 503 is formed in the upper side of the placing plate 502, a clamping mechanism 504 which is matched with the placing plate 502 to clamp and fix the reagent bottles is arranged on the outer side of the placing groove 403, the clamping mechanism 504 comprises a clamping arc plate 505, one end of the clamping arc plate 505 is rotatably connected to one end of the placing groove 403, a clamping hole 506 is formed in one side of the placing groove 403, a clamping spring 507 is fixedly connected to the inside of the clamping hole 506, the distal end of the clamping spring 507 is fixedly connected to the inner side of the clamping arc plate 505, a clamping component 508 is arranged at the distal end of the placing groove 403, the clamping component 508 comprises a pressing oblique block 509, a pressing groove 510 is formed in one end of the placing groove 403, and the pressing oblique block 509 is slidably connected to the inside of the pressing groove 510, the utility model discloses a landing gear, including pressing groove 510, pressing spring 511, limiting groove 503, landing gear 402, the inboard of spacing groove 503 is opened and is had a plurality of evenly distributed secondary constant temperature tanks 512, the upside of landing gear 402 is equipped with the grabbing pole 107.
The implementation mode is specifically as follows: the hand-operated grasping grab rod 107 and control electric telescopic rod 401 to drive undercarriage 402 and lift, place the reagent bottle inside the spacing groove 503 of placing board 502, rotate and press from both sides tight arc board 505 and make the tight reagent bottle upper end of clamp arc board 505 clamp, and reach and press down sloping block 509 position, under the inclined plane effect of pressing down sloping block 509, can promote to press down sloping block 509 and get into and press down the inside compression pressing spring 511 of groove 510, press down spring 511 to reset when pressing from both sides tight arc board 505 and laminating undercarriage 402 position completely and push out the pressing block, can press down clamping arc board 505 to press down fixed the clamping of accomplishing reagent bottle of lifting fixed, after accomplishing water bath heating, can control undercarriage 402 once more and play, manual promotion pressing block compresses pressing spring 511 once more, can push down clamping arc board 505 and break away from standing groove 403 under the extension effect that resets of clamping spring 507, namely can collect the reagent bottle.
The control mechanism 6 comprises a control frame 601, one side of the control frame 601 is rotatably connected with a plurality of synchronizing frames 602, the inner side of the control frame 601 is rotatably connected with a plurality of synchronizing gears 603, the synchronizing gears 603 are fixedly connected with the synchronizing frames 602 respectively, one side of the control frame 601 is provided with an electric slide rail 604, the moving end of the electric slide rail 604 is fixedly connected with a plurality of control gear mechanisms 605, the control gear mechanisms 605 are meshed with the synchronizing gears 603 respectively, and the inner side size of the synchronizing frames 602 is attached to one end of the undercarriage 402.
The implementation mode is specifically as follows: under the removal effect of electronic slide rail 604, drive a plurality of control gear mechanism 605 and carry out synchronous motion, can drive a plurality of synchronizing rack 602 and remove under the meshing effect of a plurality of control gear mechanism 605 and a plurality of synchronizing gear 603, when carrying out unilateral reciprocating motion and carrying out the oscillation operation, control electric telescopic handle 401 after accomplishing the tight fixed undercarriage 402 that descends of clamp of reagent bottle, move undercarriage 402 one end to the inside of synchronizing rack 602, can when carrying out reciprocating motion oscillation reagent bottle and reacing both ends, control synchronizing rack 602 drives undercarriage 402 and carries out the upset of certain angle, avoid the warm water splash to contact the reagent bottleneck and lead to the pollution, can push down the rack 501 to the backup pad 105 upside completely when multi-direction reciprocating motion is required and accomplish fixedly, can carry out multi-direction reciprocating motion oscillation.
The working principle is as follows: the water bath box 1 is placed at a required position in a laboratory, warm water is injected into the water bath box 1, cells and culture solution which need to be heated are injected into a reagent bottle, the reagent bottle is fixed in the placing mechanism 5 and the electric telescopic rod 401 is controlled to complete position adjustment, when unidirectional reciprocating oscillation is needed, only the opening and closing mechanism 2 is started to perform unidirectional reciprocating oscillation on the reagent bottle, the reagent bottle is assisted to self-adaptively turn over for a certain angle when moving to two ends under the action of the control mechanism 6, so that pollution is avoided, when other oscillation directions are needed, the auxiliary mechanism 3 is started again, the auxiliary mechanism 3 can perform multidirectional reciprocating motion under the action of the opening and closing mechanism 2, the opening and closing motor 203 is started to drive the opening and closing gear 201 to rotate, under the limit action of the rotary frame 103 and the two limit gears 202 on the two opening and closing frames 104, through the meshing action of the opening and closing gear 201 and the opening and closing gear structure 204, namely, the two supporting plates 105 can be controlled to synchronously approach and separate to complete the oscillation operation of the reciprocating motion, in the process, the moving directions of the two supporting plates 105 are opposite, the oscillation of the whole mechanism is avoided to influence the oscillation condition of warm water, and further the water bath effect is influenced, when the auxiliary frame 301 is fixed at the two ends of the water bath tank 1, the auxiliary frame 301 can be matched with the opening and closing mechanism 2 to complete the unidirectional reciprocating motion of the supporting plates 105 under the limiting action of the auxiliary frame 301 on the supporting plates 105, when the multi-directional reciprocating motion oscillation is needed, the driving motor 308 is controlled to drive the driving cylinder 305 to rotate, the auxiliary screw rod 302 is controlled to synchronously rotate under the meshing transmission action of the driving bevel gear 307 and the driven bevel gear 306 to drive the two auxiliary frames 301 to synchronously move in opposite directions, and the opening and closing mechanism 2 can be matched to control the supporting plates 105 to complete the multi-directional reciprocating oscillation motion, the grabbing rod 107 is manually grasped, the electric telescopic rod 401 is controlled to drive the undercarriage 402 to be lifted, reagent bottles are placed in the limiting groove 503 of the placing plate 502, the clamping arc plate 505 is rotated to enable the clamping arc plate 505 to clamp the upper end of the reagent bottles and reach the position of the pressing inclined block 509, the pressing inclined block 509 can be pushed to enter the pressing groove 510 to compress the pressing spring 511 under the action of the inclined surface of the pressing inclined block 509, the pressing spring 511 resets to push the pressing block out when the clamping arc plate 505 is completely attached to the position of the undercarriage 402, the clamping arc plate 505 can be pressed and fixed to finish clamping and fixing the reagent bottles, after water bath heating is finished, the undercarriage 402 can be controlled to be lifted again, the pressing spring 511 is manually pushed to compress the pressing block again, the clamping arc plate 505 can be pushed to be separated from the placing groove 403 under the resetting and extending action of the clamping spring 507, the reagent bottles can be collected, and under the moving action of the electric sliding rail 604, drive a plurality of control gear mechanisms 605 and carry out synchronous motion, can drive a plurality of synchronizing rack 602 and remove under the meshing effect of a plurality of control gear mechanisms 605 and a plurality of synchronizing gear 603, when carrying out the folk prescription to reciprocating motion and carrying out the oscillation operation, control electric telescopic handle 401 descending undercarriage 402 after accomplishing the tight fixed of clamp of reagent bottle, move undercarriage 402 one end to the inside of synchronizing rack 602, can be when carrying out reciprocating motion oscillation reagent bottle and reacing both ends, control synchronizing rack 602 drives undercarriage 402 and carries out the upset of certain angle, avoid the warm water to splash and contact the reagent bottleneck and lead to the pollution, can press down rack 501 to backup pad 105 upside completely when needing multi-direction reciprocating motion and accomplish fixedly, can carry out multi-direction reciprocating motion oscillation.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered as the technical solutions and the inventive concepts of the present invention within the technical scope of the present invention.
Claims (10)
1. The temperature regulating device for cell culture is characterized by comprising a water bath tank (1), wherein a rotating opening (101) is formed in the bottom side of the water bath tank (1), a rotating plate (102) is rotatably connected to the outer side of the rotating opening (101), a rotating frame (103) is fixedly connected to the outer side of the rotating plate (102), opening and closing frames (104) are respectively and slidably connected to the two sides of the rotating frame (103), a supporting plate (105) is rotatably connected to the far end of each opening and closing frame (104), an opening and closing mechanism (2) for controlling the two opening and closing frames (104) to synchronously approach and separate is arranged in the rotating frame (103), an auxiliary mechanism (3) which is matched with the opening and closing mechanism (2) to control the two supporting plates (105) to synchronously move in a reverse direction is arranged on the inner side of the water bath tank (1), and a plurality of working mechanisms (4) are arranged on the upper side of the supporting plate (105);
operating device (4) include two electric telescopic handle (401) and undercarriage (402), two electric telescopic handle (401) one end fixed connection is in backup pad (105) upside, undercarriage (402) both ends are rotated respectively and are connected in two electric telescopic handle (401) distal ends, undercarriage (402) one side is opened there are a plurality of evenly distributed's standing groove (403), standing groove (403) outside is equipped with placement mechanism (5), backup pad (105) upside is equipped with control mechanism (6) that control a plurality of undercarriage (402) carry out synchronous revolution, backup pad (105) are opened there are a plurality of evenly distributed's elementary constant temperature tank (106).
2. The temperature control device for cell culture according to claim 1, wherein the opening and closing mechanism (2) comprises an opening and closing gear (201) and two limit gears (202), the opening and closing gear (201) is rotatably connected inside the rotating frame (103), an opening and closing motor (203) is fixedly connected to the lower side of the rotating plate (102), the opening and closing motor (203) is located inside the rotating opening (101), the output end of the opening and closing motor (203) is fixedly connected to one side of the opening and closing gear (201), the two limit gears (202) are respectively rotatably connected to two ends of the rotating frame (103), opening and closing gear structures (204) are respectively arranged on the inner sides of the two opening and closing frames (104), and the opening and closing gear structures (204) are respectively meshed with the opening and closing gear (201) and the limit gears (202).
3. The temperature control device for cell culture according to claim 1, wherein the auxiliary mechanism (3) comprises two auxiliary frames (301) and two auxiliary screws (302), two auxiliary grooves (303) are respectively formed in two sides of the water bath tank (1), two ends of each auxiliary screw (302) are respectively rotatably connected to two ends of each auxiliary groove (303), two outer sides of the two auxiliary frames (301) are respectively slidably connected to the two inner sides of the two auxiliary grooves (303), two outer sides of the two auxiliary frames (301) are respectively in threaded connection with the two auxiliary screws (302), two sides of each auxiliary frame (301) are respectively slidably connected to two sides of the two support plates (105), and a driving mechanism (304) for controlling the two auxiliary screws (302) to synchronously rotate is arranged on the outer side of the water bath tank (1).
4. The temperature control device for cell culture according to claim 3, wherein the driving mechanism (304) comprises a driving cylinder (305), two ends of the driving cylinder (305) are respectively and rotatably connected to two ends of the water bath tank (1), two driven bevel gears (306) are rotatably connected to the outer side of the water bath tank (1), the two driven bevel gears (306) are respectively and fixedly connected to one ends of the two auxiliary screw rods (302), two ends of the driving cylinder (305) are respectively and fixedly connected to a driving bevel gear (307) engaged with the two driven bevel gears (306), a driving motor (308) is fixedly connected to the outer side of the water bath tank (1), and an output end of the driving motor (308) is fixedly connected to one end of the driving cylinder (305).
5. The temperature control device for cell culture according to claim 1, wherein the landing gear (402) is provided with a gripping rod (107) at the upper side.
6. The cell culture temperature control device according to claim 1, wherein the placing mechanism (5) comprises a placing rack (501), two ends of the placing rack (501) are respectively and fixedly connected to two ends of a placing groove (403), a placing plate (502) is fixedly connected to the lower side of the placing rack (501), a limiting groove (503) is formed in the upper side of the placing plate (502), and a clamping mechanism (504) which is matched with the placing plate (502) to clamp and fix the reagent bottle is arranged on the outer side of the placing groove (403).
7. The cell culture temperature control device according to claim 6, wherein the clamping mechanism (504) comprises a clamping arc plate (505), one end of the clamping arc plate (505) is rotatably connected to one end of the placement groove (403), a clamping hole (506) is formed in one side of the placement groove (403), a clamping spring (507) is fixedly connected to the inside of the clamping hole (506), the distal end of the clamping spring (507) is fixedly connected to the inner side of the clamping arc plate (505), and a clamping assembly (508) is arranged at the distal end of the placement groove (403).
8. The temperature control device for cell culture according to claim 7, wherein the clamping assembly (508) comprises a pressing inclined block (509), a pressing groove (510) is formed at one end of the placing groove (403), the pressing inclined block (509) is slidably connected inside the pressing groove (510), a pressing spring (511) is fixedly connected to one end of the pressing groove (510), and the distal end of the pressing spring (511) is fixedly connected to one end of the pressing inclined block (509).
9. The temperature control device for cell culture according to claim 6, wherein the limiting groove (503) has a plurality of secondary thermostatic grooves (512) uniformly distributed therein.
10. The temperature control device for cell culture according to claim 1, wherein the control mechanism (6) comprises a control frame (601), one side of the control frame (601) is rotatably connected with a plurality of synchronizing frames (602), the inner side of the control frame (601) is rotatably connected with a plurality of synchronizing gears (603), the synchronizing gears (603) are respectively and fixedly connected with the synchronizing frames (602), one side of the control frame (601) is provided with an electric sliding rail (604), the moving end of the electric sliding rail (604) is fixedly connected with a plurality of control gear mechanisms (605), the control gear mechanisms (605) are respectively meshed with the synchronizing gears (603), and the inner side size of the synchronizing frame (602) is attached to one end of the undercarriage (402).
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| CN202210074245.3A CN114591828B (en) | 2022-01-21 | 2022-01-21 | Temperature regulation and control device for cell culture |
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| CN202210074245.3A CN114591828B (en) | 2022-01-21 | 2022-01-21 | Temperature regulation and control device for cell culture |
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| KR20160036444A (en) * | 2014-09-25 | 2016-04-04 | 박의광 | Automatic water bath for sample preprocessor |
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| CN214915453U (en) * | 2020-12-28 | 2021-11-30 | 上海新礼泰药业有限公司 | Constant temperature water bath oscillator |
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| KR20160036444A (en) * | 2014-09-25 | 2016-04-04 | 박의광 | Automatic water bath for sample preprocessor |
| CN108841726A (en) * | 2018-07-21 | 2018-11-20 | 安徽细胞之星生物科技有限公司 | A kind of high steady, high-precision cell culture oscillator device |
| CN210448982U (en) * | 2019-08-06 | 2020-05-05 | 肖青好 | Oscillating device for pharmacy experiments |
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