CN108841726A - A kind of high steady, high-precision cell culture oscillator device - Google Patents
A kind of high steady, high-precision cell culture oscillator device Download PDFInfo
- Publication number
- CN108841726A CN108841726A CN201810807495.7A CN201810807495A CN108841726A CN 108841726 A CN108841726 A CN 108841726A CN 201810807495 A CN201810807495 A CN 201810807495A CN 108841726 A CN108841726 A CN 108841726A
- Authority
- CN
- China
- Prior art keywords
- storehouse
- cabinet
- cell culture
- turntable
- guide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000004113 cell culture Methods 0.000 title claims abstract description 16
- 230000033001 locomotion Effects 0.000 claims abstract description 42
- 230000010355 oscillation Effects 0.000 claims abstract description 39
- 230000001105 regulatory effect Effects 0.000 claims abstract description 10
- 240000007643 Phytolacca americana Species 0.000 claims description 28
- 230000000694 effects Effects 0.000 claims description 9
- 230000005540 biological transmission Effects 0.000 claims description 8
- 230000008878 coupling Effects 0.000 claims description 7
- 238000010168 coupling process Methods 0.000 claims description 7
- 238000005859 coupling reaction Methods 0.000 claims description 7
- 230000002547 anomalous effect Effects 0.000 claims description 6
- 230000000712 assembly Effects 0.000 claims description 5
- 238000000429 assembly Methods 0.000 claims description 5
- 230000033228 biological regulation Effects 0.000 claims description 4
- 230000002093 peripheral effect Effects 0.000 claims description 4
- 238000009434 installation Methods 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 9
- 239000001963 growth medium Substances 0.000 abstract description 7
- 230000001360 synchronised effect Effects 0.000 abstract description 6
- 239000007789 gas Substances 0.000 description 13
- 230000007306 turnover Effects 0.000 description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 241000237858 Gastropoda Species 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
Landscapes
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to shaken cultivation fields, in particular to a kind of high steady, high-precision cell culture oscillator device, balance including cabinet and for putting culture bottle further includes the lengthwise oscillations device that cabinet inner bottom wall is arranged in, the linkage storehouse being arranged at the top of lengthwise oscillations device, the swaying device being arranged in linkage storehouse and the driven speed regulating to drive swaying device quickly or slowly to move back and forth；Balance is in be horizontally arranged on the output end of swaying device；Linking, there are spacing for two sides opposite in cabinet respectively for the front and rear sides in storehouse；The motion profile of the motion profile of lengthwise oscillations device and swaying device constitutes cross, the present invention can be according to actual culture medium and cell situation, an oscillation method or the synchronous use of the two are only used between the two, ensure that oscillation amplitude can be maximized or be equalized with this, and any one oscillation method stability can suitably be guaranteed.
Description
Technical field
The present invention relates to shaken cultivation field, in particular to a kind of high steady, high-precision cell culture oscillator device.
Background technique
Shaken cultivation, also known as shaking flask culture.Refer to and microbial cell is inoculated in fluid nutrient medium, and is placed on shaking table
Or a kind of cultural method that failure of oscillation is not swung on oscillator.Oscillation energy comes into full contact with culture medium with oxygen, improves the confession of dissolved oxygen
Ying Liang, the thallus breeding through shaken cultivation is uniform, and culture efficiency is high, is widely used in bacterial screening and microorganism expands culture,
It is microbial physiology, biochemistry, common training method in fermentation and other life science fields.Oscillation is different from vibration,
The explanation of its concrete concept is object by a center, is constantly moved reciprocatingly.
Now there are many oscillator devices on the market, but these oscillator devices are in actual operation, due to duration of oscillation
Extend, will lead to that oscillation amplitude is unstable, to directly affect the culture medium in culture bottle, results even in culture bottle and spill,
And in the practical inoculation of cell liquid and culture medium, different oscillation amplitudes, some culture mediums can be used according to different situations
Need amplitude it is big some then, whereas if amplitude difference is excessive, culture medium and cell can be damaged instead, so existing oscillator device
Adaptability is not ideal enough.
Summary of the invention
In view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to provide a kind of high steady, high-precision cell culture oscillation
Equipment.
To solve the above problems, the present invention provides following technical scheme：
A kind of high steady, high-precision cell culture oscillator device, the balance including cabinet and for putting culture bottle further include
The lengthwise oscillations device that cabinet inner bottom wall is arranged in, the linkage storehouse being arranged at the top of lengthwise oscillations device, setting are in linkage storehouse
Swaying device and to drive the swaying device quickly or slowly driven speed regulating that moves back and forth；Balance is in
It is horizontally arranged on the output end of swaying device；Link storehouse front and rear sides respectively two sides opposite in cabinet there are
Away from；The motion profile of the motion profile of the lengthwise oscillations device and swaying device constitutes cross.
Further, the lengthwise oscillations device includes jointed gear unit, rodless cylinder, is symmetrical arranged and to hold
Two carrying sliding rails in support linkage storehouse and a driving plate being installed between two carrying sliding rails；Driving plate is held towards two
The side for carrying sliding rail is separately installed with a rack gear；The flank of tooth of two rack gears is oppositely arranged；Fixation is installed at the top of driving plate
Plate, two sides opposite in cabinet are fixedly connected respectively for the rear and front end of the fixed plate；And driving plate and fixed plate horizontal anomalous movement
Cooperation；Jointed gear unit is mounted on the top of fixed plate, and one end of jointed gear unit is connect with two rack-drivings, and
The other end is connect with the bottom driving in linkage storehouse；Rodless cylinder is arranged in the bottom of driving plate and is sequentially connected therewith.
Further, the jointed gear unit includes the arranged symmetrically and identical two groups of link assemblies of structure；Connecting rod
Component includes several spaced movable axis of the extending direction along rack gear, and all movable axis are in be hinged on fixed plate vertically
On, the lower semisection of each movable axis is arranged rack gear；Institute's rack gear is meshed with the rack gear of respective position respectively；Each activity
The upper semisection of axis is arranged with poke rod；Poke rod is stretching structure, and its free end is flexibly connected with the bottom in linkage storehouse.
Further, the poke rod is hollow structure, and its free end movable can be inserted with sub bar；Poke rod
Spring is inside installed, the both ends of spring respectively with the bottom wall and sub bar elastic connection in poke rod；Sub bar is far from poke rod
One end is equipped with the guide rod extended straight down, and the bottom axis on the top and linkage storehouse of guide rod connects, and the bottom wall in cabinet is opened
It is capable of the guide groove of horizontal anomalous movement equipped with the bottom end for guide rod.
Further, air pump is installed on the outside of the cabinet, Y-piece is provided in cabinet, the gas supply end of air pump passes through
Tee tube is connected to two inlet ends of Y-piece aperture position, and the outlet side of Y-piece is connected to rodless cylinder；Y-piece opening position
The both ends set are mounted on an air valve.
Further, the swaying device includes in the vertical hinged shaft, turntable for being set in shaft top, right
Claim the movable disk for guiding ring into and be mounted at the top of turntable that two arcuate structures of disk peripheral are set；Turntable is located at linkage
At center in storehouse, the eccentric part at the top of turntable is equipped with eccentric rod；Rectangular notch is offered at the center of movable disk, the rectangle
The both ends of notch are respectively facing two and guide ring extension into, and the length of rectangular notch and the diameter of turntable are equal；Eccentric rod is located always
In in rectangular notch and can in rectangular notch reciprocating movement；The outer peripheral surface of turntable and two guide ring into can be around itself axis
Line is rotatably assorted；It each guides at the top of ring and is provided in vertical slide bar；The both ends of movable disk are separately installed with one and lead
To frame, two slide bars cooperate in a guide housing, and also with respective guide housing activity always respectively, driving speed regulation dress
It sets and is connect with rotating shaft transmission.
Further, there are four the I-shaped saddles for being in distributed rectangular for the top installation of the movable disk；The top in linkage storehouse is
Opening setting；Balance is fixed on the top of all I-shaped saddles and is also located above the opening in linkage storehouse；Outer the two of linkage storehouse
Side is respectively arranged with a guide rail, and the two sides of balance cooperate with a guide rail activity respectively.
Further, the driven speed regulating includes motor and turbine and worm gearbox, and the lower semisection of shaft is arranged
There is umbrella tooth, output end and the umbrella tooth transmission of worm and gear gearbox cooperate；The main shaft of motor passes through shaft coupling and worm and gear
The input terminal of gearbox is driven cooperation.
Beneficial effect：A kind of high steady, high-precision cell culture oscillator device of the invention, working principle：It is put on balance
Set the culture bottle of batch；Subsequent rodless cylinder and motor synchronous are started to work；Matching between air pump and two air valves first
Conjunction is realizing that the characteristics of motion of regulation, specific work steps are：Air pump at work, passes through a matched gas
Valve is supplied to rodless cylinder, the trip gas be it is bigger, force driving plate Fast marching, in traveling, pass through rack drives
Jointed gear unit, jointed gear unit start passively, further to drive linkage storehouse and make vertical equity fast reciprocating activity, that
When storehouse of linking needs to transfer close to cabinet inner sidewall to be resetted, previous air valve stops working, another air valve is started to work,
And the stroke gas of the air valve is smaller, i.e. linkage storehouse reset turnover section dynamics is smaller, guarantees to stablize with this, turnover resets
Afterwards, the air valve previously stopped working on, and the small air valve of stroke gas stops working, and continues to realize regular oscillation；In addition motor
Main axis, worm and gear gearbox is passed to by shaft coupling, then umbrella is passed to by the output end of worm and gear gearbox
Tooth, umbrella tooth rotate, and further force dial movement, and the rotation of turntable is transported movable disk reciprocating linear by eccentric rod
It is dynamic, if that the line of motion for being defaulted as movable disk is X-direction, then causing the straight of linkage storehouse movement by jointed gear unit
Line is Y-direction, thus the two constitute it is cross, finally realize rule and high-accuracy high stable oscillation amplitude, the present invention
An oscillation method can be only used between the two or the two is synchronous uses, with this according to actual culture medium and cell situation
Ensure that oscillation amplitude can be maximized or be equalized, and any one oscillation method stability can be protected suitably
Card.
Detailed description of the invention
Fig. 1 is schematic perspective view one of the invention；
Fig. 2 is section plan of the invention；
Fig. 3 is three-dimensional cutaway view one of the invention；
Fig. 4 is schematic perspective view two of the invention；
Fig. 5 is stereo-resolution structural schematic diagram of the invention；
Fig. 6 is three-dimensional cutaway view two of the invention；
Fig. 7 is three-dimensional cutaway view three of the invention；
Fig. 8 is the enlarged drawing in Fig. 7 at A；
Fig. 9 is sectional perspective structural schematic diagram of the invention；
Description of symbols：Cabinet 1, guide groove 1a, link storehouse 1b, guide rail 1c.
Driven speed regulating 2, motor 2a, shaft coupling 2b, turbine and worm gearbox 2c.
Balance 3.
Swaying device 4, shaft 4a, umbrella tooth 4b, turntable 4c, eccentric rod 4d guide ring 4e, slide bar 4k into.
Movable disk 5, guide housing 5a, rectangular notch 5b, I-shaped saddle 5c.
Lengthwise oscillations device 6, jointed gear unit 6a, movable axis 6b, gear ring 6c, poke rod 6d, sub bar 6e, spring 6f,
Guide rod 6k.
Rodless cylinder 7 carries sliding rail 7a, driving plate 7b, rack gear 7c, fixed plate 7d.
Air pump 8, Y-piece 8a.
Specific embodiment
With reference to the accompanying drawings of the specification and embodiment, specific embodiments of the present invention are described in further detail：
Referring to figs. 1 to a kind of high steady, high-precision cell culture oscillator device shown in Fig. 9, including cabinet 1 and for putting
The balance 3 of culture bottle further includes the lengthwise oscillations device 6 that 1 inner bottom wall of cabinet is arranged in, setting at 6 top of lengthwise oscillations device
Linkage storehouse 1b, be arranged in linkage storehouse 1b in swaying device 4 and to drive swaying device 4 quickly or slowly
The driven speed regulating 2 of reciprocating motion；The front side of cabinet 1 is provided with control panel；Balance 3 is filled in swaying is horizontally arranged at
It sets on 4 output end；Linking, there are spacing for two sides opposite in cabinet 1 respectively for the front and rear sides of storehouse 1b；The lengthwise oscillations
The motion profile of the motion profile of device 6 and swaying device 4 constitutes cross.
The lengthwise oscillations device 6 includes jointed gear unit 6a, rodless cylinder 7, is symmetrical arranged and joins to support
Two carrying sliding rail 7a of a dynamic storehouse 1b and driving plate 7b being installed between two carrying sliding rail 7a；Driving plate 7b direction
The side of two carrying sliding rail 7a is separately installed with a rack gear 7c；The flank of tooth of two rack gear 7c is oppositely arranged；Driving plate 7b's
Top is equipped with fixed plate 7d, and two sides opposite in cabinet 1 are fixedly connected respectively for the rear and front end of fixed plate 7d；And it drives
Plate 7b and fixed plate 7d horizontal anomalous movement cooperate；Jointed gear unit 6a is mounted on the top of fixed plate 7d, jointed gear unit 6a
One end be sequentially connected with two rack gear 7c, and the other end is connect with the bottom driving for the storehouse 1b that links；The setting of rodless cylinder 7 exists
It the bottom of driving plate 7b and is sequentially connected therewith；The output end of rodless cylinder 7 is connecting with the bottom of driving plate 7b, no bar
Cylinder 7 works, and driving plate 7b is forced back and forth to advance, and in traveling, drives jointed gear unit 6a, link transmission machine by rack gear 7c
Structure 6a starts passively, further to drive linkage storehouse 1b and make vertical equity reciprocating movement；The storehouse 1b that links is slided in moving by carrying
Rail 7a is as guiding.
The jointed gear unit 6a includes the arranged symmetrically and identical two groups of link assemblies of structure；Link assembly includes
Several extending directions along rack gear 7c spaced movable axis 6b, all movable axis 6b are in be hinged on fixed plate 7d vertically
On, the lower semisection of each movable axis 6b is arranged rack gear 6c；Institute rack gear 6c is meshed with the rack gear 7c of respective position respectively；
The upper semisection of each movable axis 6b is arranged with poke rod 6d；Poke rod 6d be stretching structure, and its free end and linkage storehouse
The bottom of 1b is flexibly connected；The work of rodless cylinder 7, rack gear 7c advances with the synchronous vertical equity of driving plate 7b, in traveling, rack gear
The flank of tooth of 7c is engaged with gear ring 6c；The position of two rack gear 7c is one for being respectively at two link assemblies and being located remotely from each other
Side, then a column backwards rotation can occur for the rack gear 7c of two groups of link assemblies, a column are forward rotated, and are by movable axis 6b in this way
The free end of all poke rod 6d can be rotated toward the same direction point, be stretching structure, indirect structure by poke rod 6d
Straight-line trajectory is realized at the free end by poke rod 6d.
The poke rod 6d is hollow structure, and its free end movable can be inserted with sub bar 6e；In poke rod 6d
Spring 6f is installed, the both ends of spring 6f respectively with the bottom wall and sub bar 6e elastic connection in poke rod 6d；Sub bar 6e is separate
One end of poke rod 6d is equipped with the guide rod 6k extended straight down, the top of guide rod 6k and the bottom axis of linkage storehouse 1b
It connects, the bottom wall in cabinet 1 offers the guide groove 1a for capableing of horizontal anomalous movement for the bottom end of guide rod 6k；Gear ring 6c rotates,
Poke rod 6d is linked, and in poke rod 6d movement, and the free end of sub bar 6e is connecing with the bottom axis of linkage storehouse 1b, into one
One end that the free end of step bar 6e forces sub bar 6e to be plugged in poke rod 6d is passive and part pulls out poke rod 6d, i.e.,
As extension；Extended purpose is that the motion profile due to poke rod 6d is arc line shaped, but the motion profile for the storehouse 1b that links needs
What is wanted is linear reciprocation, so the cooperation of poke rod 6d and sub bar 6e in driving lever movement in a curve, are switched to directly by sub bar 6e
Line, and guide groove 1a moves sub bar 6e free end stability line.
The outside of the cabinet 1 is equipped with air pump 8, and Y-piece 8a is provided in cabinet 1, and the gas supply end of air pump 8 passes through threeway
Pipe is connected to two inlet ends of Y-piece 8a aperture position, and the outlet side of Y-piece 8a is connected to rodless cylinder 7；Y-piece 8a is opened
The both ends of mouth position are mounted on an air valve；The program being previously set, two air valves successively work in succession, specific work steps
For：Air pump 8 at work, by a matched air valve, is supplied to rodless cylinder 7, the trip gas be it is bigger,
Driving plate 7b Fast marching is forced, linkage storehouse 1b is being driven in cabinet 1 by linear reciprocating motion by poke rod 6d, is being moved to
When one pole location；The extreme position is by the actual size setting of cabinet 1 and the storehouse 1b that links, i.e., after linkage storehouse 1b movement
Close to cabinet 1 and the position to be resetted, that is, when the close position resetted, previous air valve stops working, another gas
Valve is started to work, and the stroke gas of the air valve is smaller, i.e. linkage storehouse 1b reset turnover section dynamics is smaller, is guaranteed surely with this
Fixed, after turnover resets, the air valve previously stopped working on, and the small air valve of stroke gas stops working, which can make this
Oscillation method in use, will not generate it is excessive jolt, maximum possible guarantee oscillation precision and stabilization.
The swaying device 4 includes in vertical hinged shaft 4a, the turntable 4c for being set in the top shaft 4a, symmetrical
The movable disk 5 of two arcuate structures on the periphery turntable 4c guiding ring 4e into and being mounted at the top of turntable 4c is set；Turntable 4c
At the center in linkage storehouse 1b, the eccentric part at the top of turntable 4c is equipped with eccentric rod 4d；It is offered at the center of movable disk 5
Rectangular notch 5b, the both ends of rectangular notch 5b are respectively facing two and guide ring 4e extension, the length and turntable of rectangular notch 5b into
The diameter of 4c is equal；Eccentric rod 4d be in rectangular notch 5b always and can in rectangular notch 5b reciprocating movement；Turntable
The outer peripheral surface of 4c and two guide ring 4e into and can cooperate around own axis；It each guides at the top of ring 4e and is provided in perpendicular
Straight slide bar 4k；The both ends of movable disk 5 are separately installed with a guide housing 5a, and two slide bar 4k are respectively always in a guiding
In frame 5a, and also cooperate with respective guide housing 5a activity, driven speed regulating 2 and shaft 4a are sequentially connected；Shaft 4a by
The driving of driven speed regulating 2, rotates, further such that turntable 4c is also depended on and guided into own axis, rotation
Ring 4e is as guiding；In turntable 4c rotation, eccentric rod 4d links therewith, and the active force for being biased to bar is living in rectangular notch 5b
It is dynamic, by rectangular notch 5b, 5 straight reciprocating motion of movable disk is forced, the specific stroke distances of movement are rectangular notch 5b
Length, in the diameter range that is to say turntable 4c, in the movement of movable disk 5, by guide housing 5a cooperation slide bar 4k as guiding.
There are four the I-shaped saddle 5c for being in distributed rectangular for the top installation of the movable disk 5；The top of linkage storehouse 1b is opening
Setting；Balance 3 is fixed on the top of all I-shaped saddle 5c and is also located above the opening of linkage storehouse 1b；Link the outer of storehouse 1b
Two sides are respectively arranged with a guide rail 1c, and the two sides of balance 3 cooperate with a guide rail 1c activity respectively；It is past due to movable disk 5
Linear motion, so relying on the guide rail of two sides in the movement of balance 3 by I-shaped saddle 5c by balance 3 with linear reciprocating motion
1c is as guiding basis, due to the movement of movable disk 5, in the movement of cooperation linkage storehouse 1b, further such that balance 3 is in cross
The movement of type rule, i.e. realization shaken cultivation.
The driven speed regulating 2 includes that motor 2a and turbine and worm gearbox 2c, the lower semisection of shaft 4a are arranged with
Output end and umbrella tooth the 4b transmission of umbrella tooth 4b, worm and gear gearbox cooperate；The main shaft of motor 2a passes through shaft coupling 2b and snail
The input terminal of worm and gear gearbox is driven cooperation；The main axis of motor 2a passes to worm and gear by shaft coupling 2b and becomes
Fast case, then umbrella tooth 4b is passed to by the output end of worm and gear gearbox, umbrella tooth 4b rotates, and turntable 4c is further forced to transport
It is dynamic, it is that its speed change is stablized using the purpose of worm and gear gearbox, precisely, so that stability is strong in oscillation, cooperation
Cell and culture medium come into full contact with, while precision is high.
Working principle：The culture bottle of batch is placed on balance 3；Subsequent rodless cylinder 7 and the synchronous beginning work of motor 2a
Make；Cooperation first between air pump 8 and two air valves is realizing that the characteristics of motion of regulation, specific work steps are：Air pump 8
At work, by a matched air valve, supply to rodless cylinder 7, the trip gas be it is bigger, force driving
Plate 7b Fast marching in traveling, drives jointed gear unit 6a by rack gear 7c, jointed gear unit 6a starts passively, into one
Step drives linkage storehouse 1b and makees vertical equity fast reciprocating activity, then when linkage storehouse 1b needs to transfer close to 1 inner sidewall of cabinet
When reset, previous air valve stops working, another air valve is started to work, and the stroke gas of the air valve is smaller, that is, links
1b reset turnover section dynamics in storehouse is smaller, guarantees to stablize with this, after turnover resets, the air valve previously stopped working on, stroke
The small air valve of gas stops working, and continues to realize regular oscillation；In addition the main axis of motor 2a is passed to by shaft coupling 2b
Worm and gear gearbox, then umbrella tooth 4b is passed to by the output end of worm and gear gearbox, umbrella tooth 4b rotates, further compels
Move turntable 4c, the rotation of turntable 4c passes through eccentric rod 4d by the linear reciprocating motion of movable disk 5, if that being defaulted as living
The line of motion of Moving plate 5 is X-direction, then causing the straight line of linkage storehouse 1b movement by jointed gear unit 6a is Y-direction, so
The two constitutes cross, finally realizes the oscillation amplitude of rule and high-accuracy high stable, and can be according to actual training
It supports base and the case where cell, an oscillation method or the synchronous use of the two is only used between the two, oscillation amplitude is ensured with this
It can maximize or equalize.
The above described is only a preferred embodiment of the present invention, not making any limit to technical scope of the invention
System, therefore any subtle modifications, equivalent variations and modifications to the above embodiments according to the technical essence of the invention,
In the range of still falling within technical solution of the present invention.
Claims (8)
1. a kind of high steady, high-precision cell culture oscillator device, including cabinet（1）And the balance for putting culture bottle
（3）, it is characterised in that：It further include being arranged in cabinet（1）The lengthwise oscillations device of inner bottom wall（6）, be arranged in lengthwise oscillations device
（6）The linkage storehouse at top（1b）, setting linkage storehouse（1b）Interior swaying device（4）And to drive swaying to fill
It sets（4）The driven speed regulating quickly or slowly moved back and forth（2）；Balance（3）In being horizontally arranged at swaying device（4）'s
On output end；Linkage storehouse（1b）Front and rear sides respectively with cabinet（1）There are spacing for interior opposite two sides；The lengthwise oscillations dress
It sets（6）Motion profile and swaying device（4）Motion profile constitute it is cross.
2. a kind of high steady, high-precision cell culture oscillator device according to claim 1, it is characterised in that：It is described vertical
To oscillation device（6）Including jointed gear unit（6a）, rodless cylinder（7）, be symmetrical arranged and to support link storehouse（1b）
Two carrying sliding rails（7a）And it is installed in two carrying sliding rails（7a）Between a driving plate（7b）；Driving plate（7b）Court
To two carrying sliding rails（7a）Side be separately installed with a rack gear（7c）；Two rack gears（7c）The flank of tooth be oppositely arranged；It drives
Movable plate（7b）Top fixed plate is installed（7d）, the fixed plate（7d）Rear and front end respectively with cabinet（1）Interior opposite two
Side is fixedly connected；And driving plate（7b）With fixed plate（7d）Horizontal anomalous movement cooperation；Jointed gear unit（6a）It is mounted on fixed plate
（7d）Top, jointed gear unit（6a）One end and two rack gears（7c）Transmission connection, and the other end and linkage storehouse
（1b）Bottom driving connection；Rodless cylinder（7）It is arranged in driving plate（7b）Bottom and be sequentially connected therewith.
3. a kind of high steady, high-precision cell culture oscillator device according to claim 2, it is characterised in that：The company
Bar transmission mechanism（6a）Including the arranged symmetrically and identical two groups of link assemblies of structure；Link assembly includes several along rack gear
（7c）The spaced movable axis of extending direction（6b）, all movable axis（6b）Fixed plate is hinged in vertical（7d）On,
Each movable axis（6b）Lower semisection be arranged rack gear（6c）；Institute's rack gear（6c）Respectively with the rack gear of respective position（7c）Phase
Engagement；Each movable axis（6b）Upper semisection be arranged with poke rod（6d）；Poke rod（6d）For stretching structure, and its freedom
End and linkage storehouse（1b）Bottom be flexibly connected.
4. a kind of high steady, high-precision cell culture oscillator device according to claim 3, it is characterised in that：Described group
Lever（6d）For hollow structure, and its free end movable can be inserted with sub bar（6e）；Poke rod（6d）Bullet is inside installed
Spring（6f）, spring（6f）Both ends respectively with poke rod（6d）Interior bottom wall and sub bar（6e）Elastic connection；Sub bar（6e）Far
From poke rod（6d）One end the guide rod extended straight down is installed（6k）, guide rod（6k）Top and linkage storehouse（1b）
Bottom axis connect, cabinet（1）Interior bottom wall is offered for guide rod（6k）Bottom end be capable of the guide groove of horizontal anomalous movement（1a）.
5. a kind of high steady, high-precision cell culture oscillator device according to claim 2, it is characterised in that：The machine
Case（1）Outside air pump is installed（8）, cabinet（1）Inside it is provided with Y-piece（8a）, air pump（8）Gas supply end by tee tube with
Y-piece（8a）Two inlet ends of aperture position are connected to, Y-piece（8a）Outlet side and rodless cylinder（7）Connection；Y-piece
（8a）The both ends of aperture position are mounted on an air valve.
6. a kind of high steady, high-precision cell culture oscillator device according to claim 1, it is characterised in that：The cross
To oscillation device（4）Including in vertical hinged shaft（4a）, be set in shaft（4a）The turntable on top（4c）, be symmetricly set on
Turntable（4c）Two arcuate structures on periphery guide ring into（4e）And it is mounted on turntable（4c）The movable disk at top（5）；Turntable
（4c）Positioned at linkage storehouse（1b）At interior center, turntable（4c）The eccentric part at top is equipped with eccentric rod（4d）；Movable disk（5）'s
Rectangular notch is offered at center（5b）, the rectangular notch（5b）Both ends be respectively facing two and guide ring into（4e）Extend, rectangle
Notch（5b）Length and turntable（4c）Diameter it is equal；Eccentric rod（4d）Always it is in rectangular notch（5b）It is interior and can be
Rectangular notch（5b）Interior reciprocating movement；Turntable（4c）Outer peripheral surface and two guide ring into（4e）It can match around own axis
It closes；Each guide ring into（4e）Top be provided in vertical slide bar（4k）；Movable disk（5）Both ends be separately installed with one
Guide housing（5a）, two slide bars（4k）It is in a guide housing always respectively（5a）It is interior, and also with respective guide housing（5a）
Activity cooperation, driven speed regulating（2）With shaft（4a）Transmission connection.
7. a kind of high steady, high-precision cell culture oscillator device according to claim 6, it is characterised in that：The work
Moving plate（5）Top installation there are four be in distributed rectangular I-shaped saddle（5c）；Linkage storehouse（1b）Top be open setting；Balance
（3）It is fixed on all I-shaped saddles（5c）Top and be also located at linkage storehouse（1b）Open top；Linkage storehouse（1b）It is outer
Two sides are respectively arranged with a guide rail（1c）, balance（3）Two sides respectively with a guide rail（1c）Activity cooperation.
8. a kind of high steady, high-precision cell culture oscillator device according to claim 7, it is characterised in that：The drive
Dynamic speed regulation device（2）Including motor（2a）With turbine and worm gearbox（2c）, shaft（4a）Lower semisection be arranged with umbrella tooth
（4b）, the output end and umbrella tooth of worm and gear gearbox（4b）Transmission cooperation；Motor（2a）Main shaft pass through shaft coupling（2b）
It is driven and cooperates with the input terminal of worm and gear gearbox.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810807495.7A CN108841726B (en) | 2018-07-21 | 2018-07-21 | High-stability and high-precision cell culture oscillation equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810807495.7A CN108841726B (en) | 2018-07-21 | 2018-07-21 | High-stability and high-precision cell culture oscillation equipment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108841726A true CN108841726A (en) | 2018-11-20 |
| CN108841726B CN108841726B (en) | 2021-06-11 |
Family
ID=64196735
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810807495.7A Expired - Fee Related CN108841726B (en) | 2018-07-21 | 2018-07-21 | High-stability and high-precision cell culture oscillation equipment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108841726B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110975722A (en) * | 2019-12-23 | 2020-04-10 | 张娣 | Constant-temperature oscillation device for drug-loaded embolism microspheres |
| CN113353307A (en) * | 2021-06-03 | 2021-09-07 | 北京君立康生物科技有限公司 | Material split charging equipment |
| CN114591828A (en) * | 2022-01-21 | 2022-06-07 | 长春中医药大学 | Temperature regulation and control device for cell culture |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202226854U (en) * | 2011-10-15 | 2012-05-23 | 四川制药制剂有限公司 | Vibrator for horizontal reciprocating movement |
| KR20130139051A (en) * | 2012-06-12 | 2013-12-20 | 고광진 | Multifunctional incubator |
| CN104762199A (en) * | 2014-01-07 | 2015-07-08 | 北京鼎昊源科技有限公司 | Freeze thawing cell oscillating breaking instrument |
| CN205821307U (en) * | 2016-07-11 | 2016-12-21 | 北京中农富源生物工程技术有限公司 | A kind of shaken cultivation case |
| US20180002650A1 (en) * | 2016-07-02 | 2018-01-04 | Zhengzhou Viri Biotech Co.,Ltd. | Shaker capable of being used in carbon dioxide incubator and cell suspension culture method |
-
2018
- 2018-07-21 CN CN201810807495.7A patent/CN108841726B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202226854U (en) * | 2011-10-15 | 2012-05-23 | 四川制药制剂有限公司 | Vibrator for horizontal reciprocating movement |
| KR20130139051A (en) * | 2012-06-12 | 2013-12-20 | 고광진 | Multifunctional incubator |
| CN104762199A (en) * | 2014-01-07 | 2015-07-08 | 北京鼎昊源科技有限公司 | Freeze thawing cell oscillating breaking instrument |
| US20180002650A1 (en) * | 2016-07-02 | 2018-01-04 | Zhengzhou Viri Biotech Co.,Ltd. | Shaker capable of being used in carbon dioxide incubator and cell suspension culture method |
| CN205821307U (en) * | 2016-07-11 | 2016-12-21 | 北京中农富源生物工程技术有限公司 | A kind of shaken cultivation case |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110975722A (en) * | 2019-12-23 | 2020-04-10 | 张娣 | Constant-temperature oscillation device for drug-loaded embolism microspheres |
| CN110975722B (en) * | 2019-12-23 | 2020-10-23 | 邹娟 | Constant-temperature oscillation device for drug-loaded embolism microspheres |
| CN113353307A (en) * | 2021-06-03 | 2021-09-07 | 北京君立康生物科技有限公司 | Material split charging equipment |
| CN114591828A (en) * | 2022-01-21 | 2022-06-07 | 长春中医药大学 | Temperature regulation and control device for cell culture |
| CN114591828B (en) * | 2022-01-21 | 2024-04-26 | 长春中医药大学 | Temperature regulation and control device for cell culture |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108841726B (en) | 2021-06-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108841726A (en) | A kind of high steady, high-precision cell culture oscillator device | |
| CN108993277A (en) | A kind of movable type agricultural liquids fertilizer mixing arrangement | |
| CN108660056A (en) | A kind of swing type shaking table for microculture | |
| CN208526659U (en) | A kind of multi-channeled transposition arrangement | |
| CN102059195A (en) | Multi-shaft and multi-head semi-automatic glue dispenser | |
| CN110195012A (en) | A kind of microorganism cultivates detection device and microculture detection method automatically | |
| CN113736622A (en) | High-speed biological shaking table driven by double motors | |
| CN105457877B (en) | Multimode oscillation mechanism | |
| CN202754415U (en) | Conveying device of steel belt | |
| CN113150960B (en) | Medical clinical microbiological detection sampling device | |
| CN205077066U (en) | Shaking table for low biology that strikes experiments | |
| CN209890609U (en) | Cell extraction injection mobile device | |
| CN203798652U (en) | Liquid supply and drainage needle dial device for sample dyeing and sample dyeing sheet pushing device | |
| CN218321179U (en) | Realize invariable organic fertilizer fermentation of gas concentration with exploding oxygen device | |
| CN208562325U (en) | A kind of stabilization shaking table for medical biotechnology culture | |
| CN118001987B (en) | Magnetic stirrer for vaccine culture solution | |
| CN203498392U (en) | Lifting liquid intermittent-impregnation automatic-cyclic culture device | |
| CN221011115U (en) | Strawberry cultivation device | |
| CN221166550U (en) | Platelet lysate releasing device for cell culture | |
| CN201829620U (en) | Graduation-displayed scale zooming device of electrically-adjusting antenna | |
| CN214810977U (en) | Automatic liquid-transfering gun mechanism | |
| CN216415485U (en) | Hot pepper soilless culture device | |
| CN215421607U (en) | Rice transplanter for planting wide and narrow planting spaces | |
| CN220987031U (en) | Water planting automated system | |
| CN219047962U (en) | Telescopic exhibition stand |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20221118 Address after: 241200 room 912a, comprehensive service center, Fanchang Economic Development Zone, Wuhu City, Anhui Province Patentee after: Wuhu cell star Bioengineering Research Co.,Ltd. Address before: Room 210-d8, A3 / F, Hefei Innovation Industrial Park, 800 Wangjiang West Road, high tech Zone, Hefei City, Anhui Province, 230088 Patentee before: ANHUI XIBAOZHIXING BIO-TECH Co.,Ltd. |
|
| TR01 | Transfer of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210611 |