CN104762199A - Freeze thawing cell oscillating breaking instrument - Google Patents
Freeze thawing cell oscillating breaking instrument Download PDFInfo
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- CN104762199A CN104762199A CN201410004731.3A CN201410004731A CN104762199A CN 104762199 A CN104762199 A CN 104762199A CN 201410004731 A CN201410004731 A CN 201410004731A CN 104762199 A CN104762199 A CN 104762199A
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Abstract
The invention relates to a freeze thawing cell oscillating breaking instrument capable of automatically swinging to break freeze thawing cells. The freeze thawing cell oscillating breaking instrument is provided with a pair of symmetric rocker arms, and the motion directions of the two rocker arms are opposite so as to offset swing and displacement of the overall instrument; a rocking support matched with cell containers is arranged on each rocker arm, and the multiple cell containers can be fixedly installed on each support; and according to the freeze thawing cell oscillating breaking instrument, a CPU control system conducts programming control over the rocking frequency and the rocking time. An implementation method is characterized in that the rocker arms are connected with a motor through a crank shaft, a deceleration belt wheel, a belt and a motor shaft sleeve; rotation of the motor is converted into horizontal motion of the rocker arms; and the two rocker arms are symmetrically installed on the crank shaft. The two supports for bearing the cell containers are fixedly installed on the rocker arms, and the supports and the rocker arms fixed to the supports move in the same direction and with the same frequency. The multiple cell containers can be fixed to each support.
Description
Technical field
The present invention relates to a kind of biotechnology particularly field of biological pharmacy instrument, be particularly useful for the device cell oscillation of cryogenic freezing being clashed into quick crashing.
Background technology
Cell breaking technology refers to and utilizes outside destroy cytolemma and cell walls, the technology making entocyte comprise object product component to discharge.The object of smudge cells mainly contains two, and one is the inclusion obtaining cell, and two is obtain cell membrane material.No matter be that a kind of object, obtaining maximum percentage of damage and maximum crushing efficiency is the target that all crushing technologies are pursued.
Different cells, its cytoclastic difficulty or ease differ, and the method for use is not identical yet.Bacterium, yeast, fungi, plant have cell walls, but composition is different, and the reticulated structure that same cells is formed is different, and therefore the firmness degree of its cell walls is different.Though zooblast does not have cell walls, have cytolemma, in addition, broken object is different, if any need extract DNA, needing of having extracts RNA or albumen, and the breaking method used also can be different.
Develop various kinds of cell breaking method at present.Breaking method can be summarized as mechanical process and the large class of on-mechanical method two.Mechanical process has high-pressure homogenization crush method (homogenization), vibration pearl hits crush method (Shaking Bead), ultrasonic fragmentation (ultrasonic) etc., on-mechanical method has osmotic shock crush method (osmotic shock), freeze thawing crush method (freezing and thawing), chemically fragmenting method (chemical treatment) etc.
It is in microtest tube, add tissue or cell and grinding bead that vibration pearl hits crush method, and test tube is with the vibration at a high speed of horizontal or vertical direction, and in vitro grinding bead is clashed into shearing cell thus reached the object of smudge cells.There are German Retsch company (horizontal oscillations) and SPEX SamplePrep company of the U.S. (vertically vibrating) in the typical instrument producer of sharp smudge cells in this way.But this method is only suitable for the fragmentation of a small amount of sample in laboratory.
Freeze thawing crush method is put by cell freezing at low temperatures (less than-15 DEG C), then melts at room temperature, repeated multiple times and reach broken wall effect.Due to freezing, can make the hydrophobic bond structure breaking of cytolemma on the one hand, thus increase the hydrophilicity of cell, water crystallization in born of the same parents, forms ice crystal grain, causes cell expansion and break on the other hand.Its shortcoming is consuming time, power consumption, and efficiency is low.
At present, in the production of modern biotechnology pharmacy or vaccine, batch smudge cells mainly adopts high-pressure homogenization crush method and chemically fragmenting method.These methods are as more satisfactory in yeast, the isocellular crushing effect of bacterium to microorganism cells, but to zooblast particularly human diploid cell, high-pressure homogenization crush method and chemically fragmenting method are too violent, broken after product (as virus or albumen) survival rate or activity not high.And freeze-thaw method is relatively applicable to the fragmentation of zooblast.
Present invention incorporates the advantage of mechanical process and freeze-thaw method smudge cells, provide a kind of mechanize to shake the device of broken frozen-thawed cell.
Summary of the invention
The technical problem to be solved in the present invention is, for biotechnology and field of biological pharmacy, to zooblast particularly human diploid cell do not have good automatization batch smudge cells technical device problem, the present invention is according to cytoclastic principle, combine mechanical oscillation and clash into broken and cell freeze thawing breaking method, providing a kind of programmable shake and clash into the device of broken frozen-thawed cell--frozen-thawed cell vibrates broken instrument, this device can once shake multiple cell container, realizes batch production.
The technical solution adopted for the present invention to solve the technical problems is: the two cover culturing bottle bracket components constructing a kind of frozen-thawed cell and to vibrate broken instrument, comprise base plate, be arranged on driven unit on described base plate and oscillation component and driven by described oscillation component.The moment of motor is delivered on the speed reducing pulley of driven unit by belt by driven unit, speed reducing pulley is connected with the bent axle in oscillation component, bent axle is fixed on base plate by bearing, bearing support, and bent axle vibrates about driving actuating arm to make oscillation component by the oscillating bearing on it.
Because distinctive mode of oscillation makes two culturing bottle bracket component direction of motion contrary, thus ensure motion barycenter geo-stationary thus offset rocking and displacement of complete machine.
This culturing bottle support can install fixing multiple cell container, is firmly arranged on culturing bottle support by the press strip of the pressing plate above culturing bottle and both sides.
In such scheme, rocking arm is connected with motor by bent axle, speed reducing pulley, belt, motor shaft sleeve, the rotation of motor is converted to the tangential movement of rocking arm, and the shake radius of rocking arm equals the radius of bent axle; The shake frequency (γ) of rocking arm and the rotating speed (V) of motor, motor shaft sleeve diameter (d1), ratio gear diameter (d2) are relevant.Relational expression is: γ=V × (d2/d1); Due to the main dependent cells of cytoclasis of freezing rear formation ice crystal and the impact force of cell container sidewall, so the shake radius of rocking arm should be greater than the radius of cell container in its direction of motion, and shake frequency is larger, and cell is easier to be broken.
In such scheme, two rocking arm symmetries are installed on bent axle, and two rocker arms roll directions are contrary, to offset rocking and displacement of complete machine device in shake process.
In such scheme, the supports of two carrying cell containers are fixedly mounted on two rocking arms, and equidirectional, the same frequency of rocking arm that support is fixed with it moves.Cell container fixedly secures according to the shape of cell container by the support of cell container.Each support can fix multiple cell container.
In such scheme, pilot circuit comprises power input unit, main control unit, electric-motor drive unit, velocity feedback unit and actuator unit.
The vibrate know-why of broken instrument of frozen-thawed cell of the present invention is that intracellular moisture and Extracellular solution form ice crystal at low temperatures, ice crystal in the oscillatory process of cell container, between ice crystal cell and ice crystal cell and cell container wall to clash into thus by cytoclasis.
The invention has the beneficial effects as follows, combine the advantage of mechanical process and freeze-thaw method smudge cells, can also once shake multiple cell container, realize batch production.
Accompanying drawing explanation
Below in conjunction with drawings and Examples, the present invention is further described.
Fig. 1 is one embodiment of the invention schematic circuit diagram
Fig. 2 is one embodiment of the invention physical construction sketch
Fig. 3 is one embodiment of the invention stereographic map
Fig. 4 is the forward sectional view of one embodiment of the invention culturing bottle support
Fig. 5 is the side sectional view of the forward sectional view of one embodiment of the invention culturing bottle support
In figure: 1. master rocker, 2. the second back shaft, 3. the first secondary-rocker, 4. the first pallet, 5. the second secondary-rocker, 6. the 3rd back shaft, 7. the second tray rack, 8. bent axle, 9. oscillating bearing, 10. the first back shaft 1, 11. left bracket for cell culture bottles, 12. right bracket for cell culture bottles, 13. rocking arm retaining plates, 14. rubber absorbers, 15. base plates, 16. actuating arms, 17. motors, 18. belts, 19. Hall speedometers, 20. speed reducing pulley, 21. hands twist cap, 22. pressing plates, 23. pressing plate rubber, 24. Tissue Culture Flasks, 25. depression bars, 26. press strips, 27. base plate rubber, 28. culturing bottle support base plates, India-rubber strip after 29., India-rubber strip before 30..
Embodiment
As shown in Figure 2 and Figure 3, motor (17) is by belt (18) and the revolution of speed reducing pulley (20) band dynamic crankshaft (8) complete cycle, and bent axle (8) drives master rocker (1) to swing by oscillating bearing (9).Actuating arm (6) and the first back shaft 1(10) and master rocker (1) be rigidly connected.First secondary-rocker (3), the second secondary-rocker (5) form with master rocker (1) parallelogram lindage that two overlap Planar Mechanisms by the first pallet (4), the second tray rack (7), wherein with the first back shaft 1(10), the second back shaft (2), the 3rd back shaft (6) three straight lines formed up and down for boundary, lower regions respectively forms a set of parallelogram lindage.First pallet (4) and the second tray rack (7) are rigidly connected with Tissue Culture Flask left socle (11) and Tissue Culture Flask right support (12) respectively.Hall speedometer (19) detects rotating speed by the magnet steel on speed reducing pulley (20) and turns back to control/driving circuit, and control/driving circuit regulates speed.
During operation, the first pallet (4) and the second tray rack (7) are taken exercises the antipodal translation in direction under the drive of rocking arm.Because the first pallet (4) is contrary with the second tray rack (7) direction of motion, what on it, load produced makes a concerted effort to be always 0, thus effectively prevent the vibration that additional dynamic load brings to instrument.
As shown in Figure 4, Figure 5, the culturing bottle fixed support in the present embodiment can fix 3 Tissue Culture Flasks (24) simultaneously.Tissue Culture Flask (24) is fixed on culturing bottle support base plate (28) by the snap-in force that depression bar (25) is downward, depression bar (25) can be moved up and down by the slotted hole of culturing bottle support base plate (28) side, thus facilitate picking and placeing (24) of Tissue Culture Flask, and twist cap (21) locking by hand.The side of Tissue Culture Flask (24) is fixed by press strip (26).Pressing plate rubber (23), rear India-rubber strip (29), base plate rubber (27), front India-rubber strip (30) can play the effect increasing frictional force and precompression, thus play better fixed effect.
Main control unit as shown in Figure 1 carries out the control of motor start and stop and travelling speed according to the speed of setting and time.Button and display section are used for speed and the setting of time and display.Velocity feedback unit Real-time Feedback speed signal is to main control unit, and main control unit carries out speed control by electric-motor drive unit to motor, and motor is again by mechanical crank-driven actuator unit.
During use, connect 220V power supply, turn on the power switch, the cycle index run by button and display-setting, the shake time of shake frequency and intermittent operation and intermittent time.These parameters can as required from Row sum-equal matrix.Then open protective guard, install and want broken culturing bottle, ensure that culturing bottle is tightened, cover protective guard.Start key on keypad, then start to run according to the program of setting with it.Can be out of service at any time by stop key in operational process, otherwise program has been run automatically out of service.
Claims (7)
1. a frozen-thawed cell vibrates broken instrument, the two cover culturing bottle bracket components comprising base plate, be arranged on driven unit on described base plate and oscillation component and driven by described oscillation component, the moment of motor is delivered on the speed reducing pulley of driven unit by belt by driven unit, speed reducing pulley is connected with the bent axle in oscillation component, bent axle is fixed on base plate by bearing, bearing support, and bent axle vibrates about driving actuating arm to make oscillation component by the oscillating bearing on it.
2. frozen-thawed cell according to claim 1 vibrates broken instrument, it is characterized in that: a kind of frozen-thawed cell broken instrument that vibrates has a pair symmetrical rocking arm, each rocking arm has the shake support mated with cell container.
3. frozen-thawed cell according to claim 1 vibrates broken instrument, it is characterized in that two rocking arms being loaded with cell container respectively do rightabout symmetry shake with the imbalance offsetting complete machine.
4. frozen-thawed cell according to claim 1 vibrates broken instrument, it is characterized in that the shake radius of rocking arm is greater than the radius in cell container direction of motion.
5. frozen-thawed cell according to claim 1 vibrates broken instrument, it is characterized in that each rocking arm can fix multiple cell container, is firmly arranged on culturing bottle support by the press strip of the pressing plate above cell container and both sides.
6. frozen-thawed cell according to claim 1 vibrates broken instrument, comprising control panel and pilot circuit, described control panel comprises button and numeral method, and control panel can set and show shake time of interval shake, intermittent time, cycle index and shake frequency.
7. frozen-thawed cell according to claim 5 vibrates broken instrument, and wherein said pilot circuit comprises power input unit, main control unit, electric-motor drive unit, velocity feedback unit and actuator unit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410004731.3A CN104762199A (en) | 2014-01-07 | 2014-01-07 | Freeze thawing cell oscillating breaking instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410004731.3A CN104762199A (en) | 2014-01-07 | 2014-01-07 | Freeze thawing cell oscillating breaking instrument |
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| Publication Number | Publication Date |
|---|---|
| CN104762199A true CN104762199A (en) | 2015-07-08 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410004731.3A Pending CN104762199A (en) | 2014-01-07 | 2014-01-07 | Freeze thawing cell oscillating breaking instrument |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108841726A (en) * | 2018-07-21 | 2018-11-20 | 安徽细胞之星生物科技有限公司 | A kind of high steady, high-precision cell culture oscillator device |
-
2014
- 2014-01-07 CN CN201410004731.3A patent/CN104762199A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108841726A (en) * | 2018-07-21 | 2018-11-20 | 安徽细胞之星生物科技有限公司 | A kind of high steady, high-precision cell culture oscillator device |
| CN108841726B (en) * | 2018-07-21 | 2021-06-11 | 安徽细胞之星生物科技有限公司 | High-stability and high-precision cell culture oscillation equipment |
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Application publication date: 20150708 |