CN111603990A - Oscillation device for genetic engineering based on rotary centrifugal force - Google Patents

Oscillation device for genetic engineering based on rotary centrifugal force Download PDF

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Publication number
CN111603990A
CN111603990A CN202010514208.0A CN202010514208A CN111603990A CN 111603990 A CN111603990 A CN 111603990A CN 202010514208 A CN202010514208 A CN 202010514208A CN 111603990 A CN111603990 A CN 111603990A
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CN
China
Prior art keywords
disc
genetic engineering
centrifugal force
test tube
shell
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Withdrawn
Application number
CN202010514208.0A
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Chinese (zh)
Inventor
黄蕊珊
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Hangzhou Leyao Biotechnology Co ltd
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Hangzhou Leyao Biotechnology Co ltd
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Priority to CN202010514208.0A priority Critical patent/CN111603990A/en
Publication of CN111603990A publication Critical patent/CN111603990A/en
Withdrawn legal-status Critical Current

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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01FMIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/20Mixing the contents of independent containers, e.g. test tubes
    • B01F31/201Holders therefor
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01FMIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/20Mixing the contents of independent containers, e.g. test tubes
    • B01F31/25Mixing the contents of independent containers, e.g. test tubes the containers being submitted to a combination of movements other than within a horizontal plane, e.g. rectilinear and pivoting movement

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)

Abstract

The invention provides a rotary centrifugal force-based oscillation device for genetic engineering, and relates to the technical field of genetic engineering. This oscillation device for genetic engineering based on rotatory centrifugal force, which comprises a housin, the top of casing is rotated and is connected with the apron, the bottom fixedly connected with motor of casing, the top transmission of motor is connected with the pivot, the peripheral fixed mounting of pivot has the disc, the peripheral fixed mounting at the inboard disc edge of casing has the spacing groove, the spout has been seted up between the inboard spacing groove of casing, the outside of disc is rotated through the fixed column and is connected with the board of placing. This oscillation device for genetic engineering based on rotatory centrifugal force can carry out stable machinery to the test tube and vibrate, in the time of more laborsaving, has also improved the effect of vibrating, has realized driving the test tube and has carried out the rotation the time, also swings the vibration to the test tube, has promoted the mixed effect of the inside sample of test tube.

Description

Oscillation device for genetic engineering based on rotary centrifugal force
Technical Field
The invention relates to the technical field of genetic engineering, in particular to a rotary centrifugal force-based oscillation device for genetic engineering.
Background
The gene engineering is also called gene splicing technology and DNA recombination technology, and is characterized by that it uses molecular genetics as theoretical basis, and uses the modern methods of molecular biology and microbiology as means, and makes the genes with different sources according to the predesigned blueprint to construct hybrid DNA molecule in vitro, then introduces it into living cell so as to change the original genetic characteristics of organism, obtain new variety and produce new product.
And in carrying out genetic engineering's experiment, in order to make the sample intensive mixing in the test tube, need vibrate the processing to the test tube, and traditional mode utilizes artifical wrist swing to realize the vibration to the test tube, not only hard, and vibrate the frequency low, the amplitude of fluctuation also is difficult to keep unanimous, the effect of vibration has been reduced, and some current mechanical oscillation devices rotate through driving the test tube and realize the mixture to the sample in the test tube, nevertheless can't realize the horizontal hunting of test tube at the pivoted in-process, make the sample can carry out the whirl in the test tube, or concentrate the outside of distributing in the test tube rotation under the effect of centrifugal force, the effect that the sample mixes has been reduced.
In order to solve the above problems, the inventor provides a vibration device for genetic engineering based on a rotational centrifugal force, which can perform stable mechanical vibration on a test tube, thereby saving labor, improving the vibration effect, driving the test tube to rotate, swinging and vibrating the test tube, and improving the mixing effect of samples in the test tube.
Disclosure of Invention
In order to achieve the purpose, the invention is realized by the following technical scheme: the utility model provides a vibration device for genetic engineering based on rotatory centrifugal force, includes casing, apron, motor, pivot, disc, spacing groove, spout, fixed column, places board, waist hole, spring, slide bar, places a section of thick bamboo, test tube, guide post.
Wherein:
the utility model discloses a test tube, including casing, fixed column, place the board, the top of casing is rotated and is connected with the apron, the bottom fixedly connected with motor of casing, the top transmission of motor is connected with the pivot, the peripheral fixed mounting of pivot has the disc, the peripheral fixed mounting at the inboard disc edge of casing has the spacing groove, the spout has been seted up between the inboard spacing groove of casing, the outside of disc is rotated through the fixed column and is connected with the board of placing, the waist hole has been seted up to the periphery of the inside fixed column of disc, place the one end fixedly connected with spring that the board is close to the disc center, the one end fixed mounting that the board kept away from the disc that places has the slide bar, the center fixed mounting that places the board places a section of.
Preferably, the limiting groove extends inwards from the inner wall of the shell and wraps the upper side and the lower side of the periphery of the disc, so that the disc can stably rotate between the limiting grooves.
Preferably, the spout is located between the spacing groove, is the wave form, and encircles and set up in the inboard of casing, the slide bar is located places board outlying center department to outwards extend, and with spout looks adaptation, consequently, when the slide bar removes the inside to the spout, place board pivoted in-process in the slide bar is followed, can reciprocate in the spout.
Preferably, the fixed columns are symmetrically distributed at the front end and the rear end of the central part of the placing plate, and are in limited sliding connection with the inner parts of the waist holes at the front side and the rear side, so that the placing plate can drive the fixed columns to move left and right in the waist holes.
Preferably, the combination of the placing plate and the placing cylinders has eight groups and is uniformly distributed on the outer side of the shell, so that the test tubes can be placed in different placing cylinders, and in the process of rotating the disc, the test tubes are rotated by the same amplitude.
Preferably, the guide posts are uniformly distributed at the upper end and the lower end of the outer side of the disc, one side, away from the disc, of each guide post is connected with a ball in a rolling mode and attached to the inner wall of the limiting groove, so that the disc can be stably positioned in the center of the limiting groove in the rotating process between the limiting grooves.
The invention provides a rotating centrifugal force-based oscillating device for genetic engineering. The method has the following beneficial effects:
1. this oscillation device for genetic engineering based on rotatory centrifugal force rotates the disc of connecting through the casing is inside to and the disc periphery places the design that a section of thick bamboo was placed to board central authorities, can stably place the test tube in placing a section of thick bamboo, recycles motor drive disc and rotates, realizes the mechanical shock to the test tube, in the more laborsaving, has also improved the effect of vibrating.
2. This vibrating device for genetic engineering based on rotatory centrifugal force, the design of the spout of connecting is encircleed in the disc outside on through shells inner wall, the slide bar in the board outside is placed in the cooperation, after the disc reachs certain rotational speed, can make and place the board and remove to the outside under the effect of centrifugal force, make the slide bar remove to the spout in, through the wavy design of spout, make the slide bar drive place the board and carry out the luffing motion, and then realized driving the test tube and carried out when rotatory, also swing the shock to the test tube, promote the mixed effect of the inside sample of test tube.
Drawings
FIG. 1 is a cross-sectional view of a structure of the present invention;
FIG. 2 is a cross-sectional view of the present invention with the disk removed;
FIG. 3 is a top view of the disk structure of the present invention;
FIG. 4 is an enlarged view of the structure of FIG. 1 at A in accordance with the present invention;
FIG. 5 is an enlarged view of the structure of FIG. 1 at high rotation speeds in accordance with the present invention;
fig. 6 is an enlarged view of the structure of fig. 3 at B according to the present invention.
In the figure: 1. a housing; 2. a cover plate; 3. a motor; 4. a rotating shaft; 5. a disc; 6. a limiting groove; 7. a chute; 8. fixing a column; 9. placing the plate; 10. a waist hole; 11. a spring; 12. a slide bar; 13. placing the cylinder; 14. a test tube; 15. and a guide post.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The embodiment of the oscillating device for genetic engineering based on the rotating centrifugal force is as follows:
referring to fig. 1-6, a shaking device for genetic engineering based on rotational centrifugal force comprises a housing 1, a cover plate 2, a motor 3, a rotating shaft 4, a disc 5, a limiting groove 6, a sliding groove 7, a fixing column 8, a placing plate 9, a waist hole 10, a spring 11, a sliding rod 12, a placing cylinder 13, a test tube 14, and a guide column 15.
Wherein:
the top end of the shell 1 is rotatably connected with a cover plate 2, the bottom of the shell 1 is fixedly connected with a motor 3, the top end of the motor 3 is in transmission connection with a rotating shaft 4, a disc 5 is fixedly arranged on the periphery of the rotating shaft 4, a limiting groove 6 is fixedly arranged on the periphery of the edge of the disc 5 on the inner side of the shell 1, the limiting groove 6 extends inwards from the inner wall of the shell 1 and wraps the upper side and the lower side of the periphery of the disc 5, so that the disc 5 can stably rotate between the limiting grooves 6, a sliding groove 7 is arranged between the limiting grooves 6 on the inner side of the shell 1, the sliding groove 7 is positioned between the limiting grooves 6 and is wavy and is arranged on the inner side of the shell 1 in a surrounding manner, a sliding rod 12 is positioned at the center of the periphery of a placing plate 9 and extends outwards and is matched with the sliding groove 7, therefore, when the sliding rod 12 moves to the inside of the sliding, the outside of disc 5 is rotated through fixed column 8 and is connected with places board 9, disc 5 through the connection of 1 inside rotation of casing to and disc 5 periphery place 9 central authorities of board and place a design of section of thick bamboo 13, can stably place test tube 14 in placing a section of thick bamboo 13, recycle motor 3 drive disc 5 and rotate, realize the mechanical shock to test tube 14, in more laborsaving, also improved the effect of shock.
The fixing columns 8 are symmetrically distributed at the front end and the rear end of the central part of the placing plate 9 and are in limited sliding connection with the inner parts of waist holes 10 at the front side and the rear side, therefore, the placing plate 9 can drive the fixing columns 8 to move left and right in the waist holes 10, the waist holes 10 are formed in the periphery of the fixing columns 8 in the disc 5, one end of the placing plate 9 close to the center of the disc 5 is fixedly connected with a spring 11, one end of the placing plate 9 far away from the disc 5 is fixedly provided with a slide bar 12, the center of the placing plate 9 is fixedly provided with a placing cylinder 13, the combination of the placing plate 9 and the placing cylinder 13 is eight groups and is uniformly distributed on the outer side of the shell 1, therefore, test tubes 14 can be placed in different placing cylinders 13, and in the rotating process of the disc 5, the test tubes 14 are placed in the placing cylinder 13 by rotation with the same amplitude, guide columns 15 are fixedly arranged at the upper end, and the guide post 15 is far away from one side roll connection of disc 5 has the ball, and it is attached mutually with the inner wall of spacing groove 6, make disc 5 carry out the in-process of rotation between spacing groove 6, guide post 15 can make disc 5 stably be in the central authorities of spacing groove 6, through the design of disc 5 outside encircleing the spout 7 of connecting on the casing 1 inner wall, the slide bar 12 in the board 9 outside is placed in the cooperation, after disc 5 reachd certain rotational speed, can make and place board 9 and remove to the outside under the effect of centrifugal force, make slide bar 12 remove to the spout 7 in, through the wavy design of spout 7, make slide bar 12 drive and place board 9 and carry out the luffing motion, and then realized driving test tube 14 and rotated the time, also carry out the swing oscillation to test tube 14, promote the mixed effect of the inside sample of test tube 14.
When the test tube placing device is used, the cover plate 2 of the shell 1 is firstly opened, then the test tubes 14 are sequentially placed in the placing barrel 13 in the center of the placing plate 9 to stably fix the test tubes 14, then the cover plate 2 is closed, the motor 3 is started to drive the rotating shaft 4 and the disc 5 to rotate, the peripheral placing plate 9 can be driven to rotate in the process of low-speed rotation of the disc 5, further the test tubes 14 are driven to rotate, the test tubes 14 are pre-stirred, after a certain rotating speed is reached, the placing plate 9 can move outwards through the tension spring 11 under the action of centrifugal force, meanwhile, the fixing columns 8 on the front side and the rear side of the placing plate 9 can be driven to move in the waist holes 10, so that the slide rods 12 on the outer side of the placing plate 9 move into the chutes 7 which are connected on the inner wall of the shell 1 in a surrounding manner, and the slide rods 12 can move up and down in the chutes 7 by matching, and then drive and place board 9 and carry out the luffing motion around fixed column 8, realize placing the vibration of a section of thick bamboo 13 interior test tube 14 to placing board 9 central authorities, improved the mixed effect to the interior sample of test tube 14, and when motor 3 rotational speed descends or stall, place board 9 and can move to the center of disc 5 under the effect of the spring 11 restoring force, make the inboard of placing board 9 move to in the disc 5, make and place board 9 and resume horizontal position, then open apron 2, the test tube 14 that will vibrate the completion takes out.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (7)

1. The utility model provides a vibrating device for genetic engineering based on rotatory centrifugal force, includes casing (1), its characterized in that: the top end of the shell (1) is rotatably connected with a cover plate (2), the bottom of the shell (1) is fixedly connected with a motor (3), the top end of the motor (3) is in transmission connection with a rotating shaft (4), a disc (5) is fixedly installed on the periphery of the rotating shaft (4), a limiting groove (6) is fixedly installed on the periphery of the edge of the disc (5) on the inner side of the shell (1), a sliding groove (7) is formed between the limiting grooves (6) on the inner side of the shell (1), the outer side of the disc (5) is rotatably connected with a placing plate (9) through a fixing column (8), a waist hole (10) is formed on the periphery of the fixing column (8) inside the disc (5), a spring (11) is fixedly connected to one end, close to the center of the disc (5), of the placing plate (9), and a sliding rod (12), the test tube placing device is characterized in that a placing barrel (13) is fixedly mounted at the center of the placing plate (9), a test tube (14) is placed inside the placing barrel (13), and guide columns (15) are fixedly mounted at the upper end and the lower end of the periphery of the disc (5).
2. The oscillating device for genetic engineering based on rotational centrifugal force of claim 1, wherein: the limiting groove (6) extends inwards from the inner wall of the shell (1) and wraps the upper side and the lower side of the periphery of the disc (5).
3. The oscillating device for genetic engineering based on rotational centrifugal force of claim 1, wherein: the sliding grooves (7) are located between the limiting grooves (6), are wavy and are arranged on the inner side of the shell (1) in a surrounding mode.
4. The oscillating device for genetic engineering based on rotational centrifugal force of claim 1, wherein: the fixing columns (8) are symmetrically distributed at the front end and the rear end of the center of the placing plate (9), and are in limited sliding connection with the inside of waist holes (10) on the front side and the rear side.
5. The oscillating device for genetic engineering based on rotational centrifugal force of claim 1, wherein: the combination of the placing plate (9) and the placing cylinder (13) is eight groups in total and is uniformly distributed on the outer side of the shell (1).
6. The oscillating device for genetic engineering based on rotational centrifugal force of claim 1, wherein: the sliding rod (12) is positioned at the center of the periphery of the placing plate (9), extends outwards and is matched with the sliding groove (7).
7. The oscillating device for genetic engineering based on rotational centrifugal force of claim 1, wherein: the guide posts (15) are uniformly distributed at the upper end and the lower end of the outer side of the disc (5), and one side, away from the disc (5), of each guide post (15) is connected with a ball in a rolling mode and attached to the inner wall of the limiting groove (6).
CN202010514208.0A 2020-06-08 2020-06-08 Oscillation device for genetic engineering based on rotary centrifugal force Withdrawn CN111603990A (en)

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CN202010514208.0A CN111603990A (en) 2020-06-08 2020-06-08 Oscillation device for genetic engineering based on rotary centrifugal force

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Application Number Priority Date Filing Date Title
CN202010514208.0A CN111603990A (en) 2020-06-08 2020-06-08 Oscillation device for genetic engineering based on rotary centrifugal force

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113441291A (en) * 2021-06-30 2021-09-28 成都导胜生物技术有限公司 Centrifugal grinding device for obtaining survival single cells
CN113680214A (en) * 2021-08-31 2021-11-23 徐世强 Microbial filtration membrane elution and separation system
CN114369709A (en) * 2021-12-22 2022-04-19 张家港市博格机械有限公司 Manufacturing process of high-strength aluminum alloy material
CN114470716A (en) * 2022-03-28 2022-05-13 枣庄学院 But angle regulation's intelligent badminton service device
CN116162539A (en) * 2023-02-15 2023-05-26 北京赛赋医药研究院有限公司 Gene sample concussion instrument

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Publication number Priority date Publication date Assignee Title
CN1924584A (en) * 2005-09-01 2007-03-07 高侨自动化科技股份有限公司 Synchronous analyzers
US20170159000A1 (en) * 2015-12-03 2017-06-08 Yury Sherman Apparatus for Mixing and Disruption of Cell and Tissue Samples in Vessels
CN207385543U (en) * 2017-10-11 2018-05-22 三峡大学 The rack for test tube that can be shaken
CN209576506U (en) * 2018-11-16 2019-11-05 南京擎科生物科技有限公司 Horizontal whirlpool rocking equipment
CN111068558A (en) * 2020-02-04 2020-04-28 青岛龙凹医疗器械有限公司 Medical constant temperature blood blending device

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1924584A (en) * 2005-09-01 2007-03-07 高侨自动化科技股份有限公司 Synchronous analyzers
US20170159000A1 (en) * 2015-12-03 2017-06-08 Yury Sherman Apparatus for Mixing and Disruption of Cell and Tissue Samples in Vessels
CN207385543U (en) * 2017-10-11 2018-05-22 三峡大学 The rack for test tube that can be shaken
CN209576506U (en) * 2018-11-16 2019-11-05 南京擎科生物科技有限公司 Horizontal whirlpool rocking equipment
CN111068558A (en) * 2020-02-04 2020-04-28 青岛龙凹医疗器械有限公司 Medical constant temperature blood blending device

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113441291A (en) * 2021-06-30 2021-09-28 成都导胜生物技术有限公司 Centrifugal grinding device for obtaining survival single cells
CN113441291B (en) * 2021-06-30 2022-07-26 成都导胜生物技术有限公司 Centrifugal grinding device for obtaining survival single cells
CN113680214A (en) * 2021-08-31 2021-11-23 徐世强 Microbial filtration membrane elution and separation system
CN114369709A (en) * 2021-12-22 2022-04-19 张家港市博格机械有限公司 Manufacturing process of high-strength aluminum alloy material
CN114470716A (en) * 2022-03-28 2022-05-13 枣庄学院 But angle regulation's intelligent badminton service device
CN116162539A (en) * 2023-02-15 2023-05-26 北京赛赋医药研究院有限公司 Gene sample concussion instrument
CN116162539B (en) * 2023-02-15 2023-07-25 北京赛赋医药研究院有限公司 Gene sample concussion instrument

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Application publication date: 20200901