CN203159593U - Rubber tree powdery mildew culturing device - Google Patents
Rubber tree powdery mildew culturing device Download PDFInfo
- Publication number
- CN203159593U CN203159593U CN 201320103639 CN201320103639U CN203159593U CN 203159593 U CN203159593 U CN 203159593U CN 201320103639 CN201320103639 CN 201320103639 CN 201320103639 U CN201320103639 U CN 201320103639U CN 203159593 U CN203159593 U CN 203159593U
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- Prior art keywords
- rubber tree
- powdery mildew
- box
- culture
- tree powdery
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a rubber tree powdery mildew culturing device. The device comprises a culture box and a culture shelf, wherein the culture box consists of a box cover and a box body, and the box body is sheathed outside the box cover; and the culture shelf is provided with grids, grooves and shelf legs, and the culture shelf is arranged in the culture box. The rubber tree powdery mildew culturing device is simple in structure, low in manufacturing cost, convenient to use, good in culture effect, can provide stable humidity environment when rubber tree leaves are utilized for powdery mildew in-vitro culture, thus being beneficial to fast growth of powdery mildew.
Description
Technical field
The utility model relates to a kind of rubber tree powdery mildew bacterium culture apparatus, belongs to the plant protection technology field.
Background technology
The rubber tree powdery mildew Pseudomonas can only be cultivated and preserve in obligate parasite at fresh and alive organization material.Under the prior art condition, have only blade when white powder infection process rubber tree, inflorescence etc. to cause morbidity and when producing fresh white powder germ spore, just can carry out experimental studies such as disease evaluation, breeding for disease resistance.Therefore, white powder germ experimental study existed be subjected to that effect of natural conditions is big, the time short, be not easy defectives such as control and preservation, make troubles to scientific effort.At present, utilize incubator isolated culture rubber tree powdery mildew bacterium in the experimental study, because of incubator isolated culture humidity not enough, excised leaf can not the long period keep vitality, can not breed a large amount of white powder germs in a short time for artificial inoculation, cause in time to solve the key issue that the rubber tree powdery mildew resistance is identified.
Summary of the invention
The purpose of this utility model provides a kind of rubber tree powdery mildew bacterium culture apparatus, to breed a large amount of white powder germs in a short time, for artificial inoculation, solves the key issue that the rubber tree powdery mildew resistance is identified.
In order to realize the purpose of this utility model, a kind of rubber tree powdery mildew bacterium of the utility model culture apparatus is formed by cultivating box and culturing rack.Described cultivation box comprises lid and box body, and breather hole is arranged on the lid, and gas port is arranged on the box wall, the lid box body that is locked outward; Culturing rack has grid, groove and frame pin, and culturing rack is positioned over to be cultivated in the box.
A breather hole is arranged on the lid of described cultivation box, be used for breathing freely, the breather hole diameter is 5～8cm, and breather hole is provided with wire netting, but placement filter cotton on the wire netting, and filter cotton is used for keeping the humidity in the box; A gas port is arranged on the box wall, be used for hose connection with humidifier.The box body that is locked beyond the lid is advisable, and makes lid cover box body completely.
The length and width height of described culturing rack is slightly less than the cultivation box, and culturing rack can be positioned over fully to be cultivated in the box; Culturing rack has grid, and grid has mesh, and mesh is square, and the length of side is 1～2cm, and respectively there is a groove grid both sides, and the groove cross section is semicircle, is used for placing rubber tree blade nutrient solution; Culturing rack has four frame pin, to keep the airiness after blade is placed.
Cultivate box and culturing rack and make respectively, but make of the transparent rigid plastics of high temperature high voltage resistant sterilization.
A kind of rubber tree powdery mildew bacterium of the utility model culture apparatus, in rubber tree powdery mildew bacterium isolated culture, stable humidity environment can be provided, for the rubber tree powdery mildew bacteria growing provides suitable humidity condition, culture of ex vivo rubber tree leaf effectively, make the rubber tree excised leaf long period to keep vitality, be beneficial to the quick growth of white powder germ, can breed a large amount of white powder germs in a short time, be used for the artificial inoculation of rubber tree breeding for disease resistance, solved the key issue that the rubber tree powdery mildew resistance is identified, having improved the not enough situation of incubator isolated culture rubber tree powdery mildew bacterium humidity, can in time be rubber tree powdery mildew morphology, biology and molecular biological research provide associated materials.The utility model is simple in structure, and cost of manufacture is low, and is easy to use, and culture effect is good, provides powerful measure for utilizing excised leaf to cultivate the rubber tree powdery mildew bacterium.
Description of drawings
Fig. 1: cultivate the box structural representation
Among the figure: 1. lid; 2. breather hole; 3. wire netting; 4. box body; 5. gas port.
Fig. 2: culturing rack structural representation
Among the figure: 6. grid; 7. mesh; 8. groove; 9. frame pin.
Embodiment
Following examples are used for explanation the utility model, but are not used for limiting scope of the present utility model.
When a kind of rubber tree powdery mildew bacterium culture apparatus of the present utility model is implemented, as depicted in figs. 1 and 2, comprise lid (1), breather hole (2), wire netting (3), box body (4), gas port (5), grid (6), mesh (7), groove (8), frame pin (9).On the lid of cultivation box a breather hole is arranged, the breather hole diameter is 6cm, is provided with wire netting in the breather hole, but placement filter cotton on the wire netting; A gas port is arranged on the box wall, can be with humidifier hose connection; The box body that is locked beyond the lid is advisable, and makes lid cover box body completely.Culturing rack has grid, and grid has mesh, and mesh is square, and the length of side is 1cm, and respectively there are a groove and two frame pin in the grid both sides, and the groove cross section is semicircle, and culturing rack can be put into the cultivation box fully and be advisable.During making, cultivate box and culturing rack and make respectively, but make of the transparent rigid plastics of high temperature high voltage resistant sterilization.
During use, handle the stripped young leaflet tablet of rubber tree earlier well, select rubber tree to be in the young leaflet tablet of variable color phase, put into 0.5% chlorine bleach liquor and soaked 5 minutes, use aseptic water washing again three times, drain.With cultivating the moist heat sterilization pot sterilization 30min that box and culturing rack are put in 121 ℃, take out oven dry, cooling.Then culturing rack is put into box body, the nutrient solution for preparing is transferred in the groove of culturing rack with pipettor, the petiole with the rubber tree young leaflet tablet handled well inserts in the nutrient solution of groove again, and the blade face places on the grid.Then be inoculated on the rubber tree young leaflet tablet with fresh white powder germ on the susceptible rubber tree blade of the light brush of writing brush, cover tightly box body with lid, place filter cotton in the breather hole of lid, the gas port of box body is with hose connection humidifier air outlet.By the humidification of humidifier and the temperature of adjusting culturing room, making the temperature of cultivating in the box is 23 ℃, humidity is 90%, cultivated 7～10 days, can generate a thick layer of white powder germ spore at the young leaflet tablet that rubber tree is exsomatized, identify for the artificial inoculation of experimental study and the resistance of rubber tree powdery mildew.
Though, above with a general description of the specific embodiments detailed description having been done in the invention, on the basis of the invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements on the basis of not departing from the invention spirit all belong to the claimed scope of the invention.
Claims (5)
1. a rubber tree powdery mildew bacterium culture apparatus is characterized in that forming by cultivating box and culturing rack; Described cultivation box comprises lid and box body, the lid box body that is locked outward; Culturing rack has grid, groove and frame pin, and culturing rack is positioned over to be cultivated in the box.
2. a kind of rubber tree powdery mildew bacterium culture apparatus according to claim 1 is characterized in that having on the lid breather hole, and diameter is 5～8cm.
3. a kind of rubber tree powdery mildew bacterium culture apparatus according to claim 1 is characterized in that the mesh of grid is square, and the length of side is 1～2cm.
4. a kind of rubber tree powdery mildew bacterium culture apparatus according to claim 1 is characterized in that respectively there is a groove grid both sides, and the groove cross section is semicircle.
5. a kind of rubber tree powdery mildew bacterium culture apparatus according to claim 1, but it is characterized in that cultivating box and culturing rack is made of the transparent rigid plastics of high temperature high voltage resistant sterilization.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320103639 CN203159593U (en) | 2013-03-06 | 2013-03-06 | Rubber tree powdery mildew culturing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320103639 CN203159593U (en) | 2013-03-06 | 2013-03-06 | Rubber tree powdery mildew culturing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203159593U true CN203159593U (en) | 2013-08-28 |
Family
ID=49020792
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320103639 Expired - Fee Related CN203159593U (en) | 2013-03-06 | 2013-03-06 | Rubber tree powdery mildew culturing device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203159593U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103937868A (en) * | 2014-05-06 | 2014-07-23 | 新疆艾比湖戈宝麻有限公司 | Method for forecasting field kendir rust disease and special device therefor |
| CN104694532A (en) * | 2015-03-25 | 2015-06-10 | 海南大学 | Extracting method of RNA of rubber tree powdery mildew |
-
2013
- 2013-03-06 CN CN 201320103639 patent/CN203159593U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103937868A (en) * | 2014-05-06 | 2014-07-23 | 新疆艾比湖戈宝麻有限公司 | Method for forecasting field kendir rust disease and special device therefor |
| CN104694532A (en) * | 2015-03-25 | 2015-06-10 | 海南大学 | Extracting method of RNA of rubber tree powdery mildew |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130828 Termination date: 20140306 |