WO2012162855A1

WO2012162855A1 - Cell cultivator

Info

Publication number: WO2012162855A1
Application number: PCT/CN2011/000924
Authority: WO
Inventors: 黄玲惠
Original assignee: Huang Lynn L H
Priority date: 2011-06-01
Filing date: 2011-06-01
Publication date: 2012-12-06
Also published as: CN103620018B; CN103620018A

Abstract

Provided is a cell cultivator, said cell cultivator includes a hollow tank, a removable membrane, a fixed sheath and a support seat, wherein the removable membrane is fixed on the bottom opening of the tank, the support seat is located on the outside of the tank and supports the tank, living cells or others biological materials can be placed on the membrane to culture. The position of the tank of said cell cultivator, relative to the support seat, can be adjusted to facilitate the microscope focusing. After the cell culture is ending, the fixed sheath can be removed from the bottom of the tank in order to remove the membrane to observe it or to use it in clinical and medical applications.

Description

Cell culture device

The present invention relates to a fine cell culture device, and more particularly to a cell culture device which can be used in the field of living cell culture, or as a carrier used in the field of microscopic observation of biological materials, in order to facilitate living cells or other Biomaterials for microscopic observation and filming of cell culture design. Moreover, the cell culture device can also be applied to in vitro or direct transplantation of a living body cell into a human or animal body, or further combined with any form of biomedical material in a cell culture device or the like. Background technique

For example, in the related fields of research and application of cell biology, for in vitro cell culture, or for tissue formation, cell line establishment, cell physiological toxicology test, cell migration observation, cell and another cell or other extracellular matrix Or various studies and applications between factors, or when it is difficult to perform a living experiment, it is usually necessary to use a cell culture device to provide the in vitro culture of stem cells and various other types of cells. The various cells described above can be further combined with any form of biomedical material and used in medical research and transplantation applications.

At present, the existing cell culture device design mainly includes a tank body having three support legs and a diaphragm, wherein the tank body forms an upper and lower through-hole accommodating space, and the diaphragm is fixed to the tank body. At the bottom opening. When the cell culture device is applied to a cell culture, the membrane attached to the bottom of the tank body is provided with a living body cell attached to the surface, and is immersed in the culture medium to allow the living body cell to grow and grow.

In the above-mentioned cell culture device, the tank body is mostly formed by casting by casting, and although the tank body adopts a tripod structure design, the medium can be provided with good fluidity on the membrane to facilitate the growth of living cells, however, The groove body manufactured by using the mold forming is a fixed shape and non-variable shape, and has no adjustability, and the diaphragm is fixed at the bottom of the tank body, and cannot be arbitrarily disassembled, when used in clinical medical applications, or desired by the user. For live cell observation or image capture, microscopic image observation must be performed together with the trough body. When using a high magnification objective lens, the high magnification lens has a large NA value and a short working distance. When the lens is adjusted in focus, the groove is located in the diaphragm. The upper obstruction, and the unfavorable microscope to obtain a clearer image of the diaphragm. Summary of the invention

The technical problem to be solved by the present invention is to improve the disadvantage that the diaphragm of the current cell culture device is fixed at the bottom of the tank body and is not easy to disassemble. In order to solve the aforementioned technical problems, the technical solution of the present invention is to provide a cell culture device comprising:

a hollow trough body, an upper opening is formed at the top of the trough body, an opening is formed at the bottom of the trough body, and a positioning portion is located at a periphery of the opening;

a diaphragm is a detachable group covering the opening at the bottom of the tank body, and the periphery of the diaphragm extends to the side of the positioning portion at the bottom of the tank body;

a fixing sleeve is a detachable group mounted on the positioning portion at the bottom of the tank body, and the diaphragm is fixed to the bottom of the tank body;

A support base includes a body, and the seat body is disposed outside the tank body to support the tank body.

In the cell culture device as described above, the base of the support base has an annular body, and the bottom edge of the f-shaped annular body extends downwardly to support a plurality of support legs, and the base body can be assembled on the outer side of the groove body in an up-and-down manner.

In the cell culture device as described above, the tip end of the base body forms an inner ring convex portion extending inward in the diameter direction, and an adjustment positioning portion formed by a plurality of convex groove intervals is formed on the inner ring periphery of the inner ring convex portion. The groove body forms a plurality of positioning concave edges arranged at an upper and lower intervals on the outer circumferential surface above the positioning portion, and provides an adjustment positioning portion to be positioned therein, so that the groove body can be raised and lowered to adjust the height.

In the cell culture device as described above, the bottom portion of the inner ring protrusion of the seat body forms a pressing portion that can push against the fixing sleeve.

In the cell culture device as described above, the positioning portion is an annular snap portion formed on the outer peripheral surface of the bottom end of the trough body, and the fixing sleeve has a set of annular sleeve body disposed on the outer periphery of the trough body, the annular sleeve body The circumferential surface forms an annular fixing portion, and the annular locking portion and the annular fixing portion are concave and convex portions forming an arc curved surface, and the fixing sleeve is fixedly fixed to the outer periphery of the groove body, and the diaphragm is clamped and fixed to the annular locking portion and Between the annular fixing parts.

In the cell culture device as described above, the 不-shaped engaging portion is an annular curved curved flange, and the annular fixing portion is an annular curved curved edge.

In the cell culture device as described above, the annular engaging portion is an annular curved curved edge, and the annular fixed portion is an annular curved curved flange. .

In the cell culture device as described above, the bottom end of the annular sleeve of the fixing sleeve forms an inwardly extending ring bottom edge, and the bottom edge of the fixing sleeve is opposite to the positioning portion of the bottom end of the groove body to sandwich the diaphragm.

In the cell culture device as described above, the annular snap portion is an annular curved curved flange, and the annular fixing portion is an annular curved curved concave edge; the bottom end of the annular sleeve of the fixed sleeve is formed inward The bottom edge of the extended ring, the bottom edge of the ring of the fixing sleeve and the positioning portion of the bottom end of the groove body are opposite to the clamping film.

In the cell culture device as described above, the annular snap portion is an annular curved curved edge, and the annular fixed portion The annular curved surface of the annular sleeve has an annular bottom edge extending inwardly, and the bottom edge of the fixing sleeve is opposite to the positioning portion of the bottom end of the groove body to sandwich the diaphragm.

The invention has the advantages that: in the cell culture process, the user can use the membrane disposed on the bottom surface of the tank body to be immersed in the proper height position of the culture medium of the tank, and the culture medium and the additive contained in the culture medium are The membrane has good permeability, so that the living cells on the surface of the membrane can be well cultured; when the user wants to observe the cells through the microscope, the annular seat body is adjusted upwards so that the bottom of the tank body can be approached or It is placed on the stage of the microscope, allowing the user to precisely adjust the focus of the microscope to achieve a better microscopic image. At the end of the cell culture, the fixed sleeve can be removed from the bottom surface of the tank, and the cell or cell-containing biomedical material can be transplanted, or the membrane can be taken out and observed directly under the objective lens of the microscope, so that the tank body and the tank can be avoided. The peripheral support block obstructs the microscope, allowing the user to precisely adjust the focus of the microscope to achieve an ideal image.

In the cell culture device of the present invention, the base body of the support base can have an annular body, and a plurality of support legs extend downwardly from the bottom edge of the annular base body, and the base body can be assembled on the outer side of the trough body by adjusting up and down. The film disposed at the bottom of the tank is easy to adjust its height position up and down. DRAWINGS

BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 is a perspective exploded view of a first preferred embodiment of the cell culture device of the present invention.

Figure 2 is a schematic plan view showing the combination of the first preferred embodiment of the cell culture device shown in Figure 1.

Figure 3 is a partially enlarged schematic view showing the first preferred embodiment of the cell culture device shown in Figure 2.

Fig. 4 is a schematic plan view showing another preferred embodiment of the preferred embodiment of the preferred embodiment of the cell culture device of Fig. 1 in which the annular snap portion and the annular fixing portion are interchanged.

Figure 5 is a schematic plan view showing a combination of a second preferred embodiment of the cell culture device of the present invention.

Figure 6 is a partially enlarged schematic view showing a second preferred embodiment of the cell culture device shown in Figure 5.

Figure 7 is a plan view showing the support stand in the first preferred embodiment of the cell culture device shown in Figure 1 ascending to lower the height of the cell culture device.

Figure 8 is a schematic view showing the operation of disassembling the first preferred embodiment of the cell culture device shown in Figure 1.

Description of the reference numerals

1 0-slot; 1 1-open; 12-positioning; 1 3, 1 3a-annular snap; 14-positioning recess; 1 5-ring flange; 20-diaphragm; 30-fixed 300-ring sleeve; 301 - ring bottom edge; 31, 31 a-ring fixing portion; 40-support seat; 41-seat body; 42-supporting foot; 4 3-inner ring convex portion; - Adjusting the positioning portion; 441 - Bump; 45 - Pressing portion. detailed description

The technical means for achieving the intended object of the invention will be further elucidated in the following description in conjunction with the drawings and preferred embodiments of the invention.

As shown in Figures 1 to 4 and Figures 5 to 6, several preferred embodiments of the cell culture device of the present invention are disclosed. As can be seen from the drawings, the cell culture device designed by the present invention is mainly The utility model comprises: a tank body 10, a diaphragm 20, a fixing sleeve 30 and a supporting base 40, wherein:

The groove body 10 is a hollow body that is vertically penetrated. An upper opening is formed at the top of the groove body 10. An opening 11 and a positioning portion 12 located at the periphery of the opening 11 are formed at the bottom of the groove body 10.

The diaphragm 20 is a porous membrane having permeability, the diaphragm 20 is a detachable group covering the opening of the bottom of the tank body 10, and the periphery of the diaphragm 20 extends to the positioning portion 12 at the bottom of the tank 10. side.

The fixing sleeve 30 is detachably assembled on the positioning portion 12 at the bottom of the groove body 10 for fixing the diaphragm 20 to the bottom of the groove body 10 in a flat manner.

The support base 40 includes a body 41, or further includes a plurality of support legs 42 extending downwardly from the bottom of the base body 41. The support base 40 is assembled to the slot body by the base body 41. 10 outside, used to support the tank 10.

As shown in FIG. 1 to FIG. 3 and FIG. 5 to FIG. 6 , the positioning portion 12 of the groove body 10 is an annular snap portion 13 formed on the outer peripheral surface of the bottom end of the groove body 10; In a preferred embodiment, the fixing sleeve 30 has an annular sleeve 300 that can be sleeved on the periphery of the groove body 10. The bottom end of the annular sleeve body 300 forms an inwardly extending ring bottom edge 301, or, as shown in FIG. To the second preferred embodiment shown in FIG. 6, the fixing sleeve 30 has an annular sleeve 300 that can be sleeved on the outer periphery of the groove body 10. The fixing sleeve 30 forms an annular solid on the inner circumferential surface of the annular sleeve 300. The connecting portion 31, the annular engaging portion 13 and the annular fixing portion 31 are concave-convex fitting structures forming an arc-shaped curved surface. In the first preferred embodiment shown in FIG. 2 or FIG. 3, the annular engaging portion 13 is a ring. The arcuate curved flange, the annular fixing portion 31 is designed as an annular curved curved edge. In another preferred embodiment shown in FIG. 4, the annular engaging portion 13a can also be annular. The annular curved portion 31a is an annular curved curved flange design, so that the annular sleeve 300 of the fixing sleeve 30 can be sleeved and fixed on the periphery of the groove 10, and the diaphragm is provided. 20 is sandwiched between the fixed annular engaging portion 13 and the annular fixing portion 31, and the annular locking portion 13 and the annular fixing portion 31 are combined with the curved surface of the curved curved surface, so that the two are not only firmly coupled The utility model has the function of being easy to assemble and disassemble; if the fixing sleeve 30 has the ring bottom edge 301 at the bottom end of the annular sleeve 300, the ring bottom edge 301 can further be opposite to the positioning portion 12 at the bottom end of the groove body 10. The diaphragm 20 is clamped.

The base body 41 of the support base 40 is an annular body. The plurality of support legs 42 are formed to extend downwardly from the bottom edge of the annular seat body 41. The base body 41 can be assembled on the outer side of the slot body 10 by adjusting up and down. As shown in Figure 2 or In the preferred embodiment shown in FIG. 3, the top end of the base 41 is formed with an inner ring protrusion 43 extending inward in the diametrical direction, and an adjustment positioning portion 44 is formed on the inner circumference of the inner ring protrusion 43. The positioning portion 44 may be formed by a plurality of convex 441s spaced apart from the inner ring circumference of the inner ring convex portion 43. The groove body 10 forms a plurality of annular positioning concave edges arranged at upper and lower intervals on the outer circumferential surface above the positioning portion 12. 14 . The positioning positioning portion 44 is configured to be positioned therein, so that the groove body 10 can be adjusted up and down relative to the support base 40. A positioning concave edge 14 of the plurality of annular positioning concave edges 14 is adjacent to the groove body 10 . In the preferred embodiment shown in FIGS. 1 to 3, the support base 40 may also form a pressing portion 45 on the bottom surface of the inner ring convex portion 43 as a portion for pushing down the fixing sleeve 30.

In the preferred embodiment shown in FIG. 1 or FIG. 2, the top edge of the groove body 10 defines a ring top flange 15, and the outer diameter of the ring top flange 15 is larger than the inner diameter of the inner ring protrusion 43 of the support base 40. The trough body 10 passes through the support base 40 from top to bottom and is restrained by the ring top flange 15 against the inner ring protrusion 43 of the support base 40.

When the cell culture device of the present invention is used, the cell culture device is immersed in a medium containing a culture solution and an additive, and the living cells (or a combination of cells and biomedical materials) to be cultured are attached to the cell culture device. The surface of the porous membrane 20, which is appropriately permeable and smooth, allows the living cells to continue to survive in the culture medium.

As shown in FIG. 7 , during the culturing process, the user can adjust the height position of the tank body 10 by using the support base 40, so that the diaphragm 20 on the bottom surface of the tank body 10 is located at an appropriate height in the medium, and the medium is included in the medium. The culture solution and the additive have good permeability in the membrane 20, so that the living cells on the surface of the membrane 20 can be well cultured.

When the user wants to observe the cells through the microscope, the annular seat body 41 is adjusted upward to make the trough body

The diaphragm 20 at the bottom of the 10 can be placed close to or placed on the stage of the microscope, allowing the user to precisely adjust the focus of the microscope to achieve a better microscopic image. As the cell culture is completed, as shown in FIG. 8, the fixing sleeve 30 can be detached from the bottom surface of the tank body 10, wherein the pressing sleeve 45 can be pushed down by the pressing portion 45 of the supporting seat 40, and the ring-shaped snap-in can be used. The portion 13 and the curved curved concave-convex structure of the annular fixing portion 31 disengage the fixing sleeve 30 from the groove body 10, and the diaphragm 20 is taken out, and the removed diaphragm 20 can be directly used for clinical cell transplantation or cell-containing organisms. The medical material is transplanted or directly observed under the objective lens of the microscope, so that the microscope 10 and its peripheral support base 40 can be prevented from obstructing the microscope, and the user can precisely adjust the microscope focus to obtain a better cell image.

In summary, the cell culture device of the present invention can solve the problem that the height of the support base on the periphery of the cell body can not be accurately focused by the microscope when the cell is observed, and the cell culture device designed by the present invention is easy to disassemble and operate the film. Therefore, in the field of cell culture technology or other biomaterials and regenerative medicine, It can provide a practical cell culture device with great industrial value.

The above description of the present invention is intended to be illustrative and not restrictive, and it will be understood by those skilled in the Within the scope of protection of the present invention.

Claims

Rights request

A cell culture device, characterized in that it comprises:

a diaphragm is a detachable opening covering the bottom of the trough body, and the periphery of the diaphragm extends to the side of the positioning portion at the bottom of the trough;

a fixing sleeve is a detachable positioning portion mounted on the bottom of the tank body, and the diaphragm is fixed to the bottom of the tank body;

2 . The cell culture device according to claim 1 , wherein: the base of the support base has an annular body, and a plurality of support legs extend downward from a bottom edge of the annular base body, and the base body can adjust the positioning group up and down. It is located outside the tank.

The cell culture device according to claim 2, wherein: the top end of the base body forms an inner ring convex portion extending inward in the diametrical direction, and the outer ring periphery of the inner ring convex portion is formed by a plurality of convex groove intervals. The adjustment positioning portion is arranged, and the groove body forms a plurality of positioning concave edges arranged at upper and lower intervals on the outer circumferential surface above the positioning portion, and the adjustment positioning portion is positioned to adjust the height of the groove body.

The cell culture device according to claim 3, wherein the bottom portion of the inner ring convex portion forms a pressing portion that can push against the fixing sleeve.

The cell culture device according to any one of claims 1 to 4, wherein the positioning portion is an annular snap portion formed on an outer peripheral surface of the bottom end of the trough body, the fixing sleeve having a set The annular sleeve body at the outer periphery of the groove body forms an annular fixing portion on the circumferential surface of the annular sleeve, and the annular locking portion and the annular fixing portion are concave and convex portions forming an arc curved surface, so that the fixing sleeve is fixed to the groove At the periphery of the body, the diaphragm is clamped between the annular snap-fit portion and the annular fixing portion.

The cell culture device according to claim 5, wherein the annular snap portion is an annular curved curved flange, and the annular fixed portion is an annular curved curved edge.

The cell culture device according to claim 5, wherein the ring engaging portion is an annular curved curved edge, and the annular fixing portion is an annular curved curved flange. .

The cell culture device according to claim 5, wherein: the bottom end of the annular sleeve of the fixing sleeve forms an inner bottom edge of the ring, and the bottom edge of the fixing sleeve is opposite to the positioning portion of the bottom end of the groove body. Hold the diaphragm.

The cell culture device according to claim 5, wherein the annular snap portion is annular The curved curved flange, the annular fixing portion is an annular curved curved edge; the bottom end of the annular sleeve of the fixing sleeve forms an inner bottom edge of the ring, and the bottom edge of the fixing sleeve and the bottom end of the groove body are positioned The part is opposite to the clamping diaphragm.

The cell culture device according to claim 5, wherein: the annular snap portion is an annular curved curved edge, and the annular fixed portion is an annular curved curved flange; The bottom end of the annular sleeve body forms an inner bottom edge of the ring, and the bottom edge of the ring of the fixing sleeve is opposite to the positioning portion of the bottom end of the groove body to sandwich the diaphragm.