200944589 九、發明說明： 【發明所屬之技術領域】 本發明係關於一種嵌入式培養槽，尤指一種可應用於 活體細胞培養領域所使用之細胞培養器具，或作為生物材 料顯微觀察領域所使用的載具，以便於活體細胞或其他生 物材料進行顯微觀察及拍攝紀錄的嵌入式培養槽設計。 【先前技術】 如細胞生物學之研究與應用等相關領域中，為進行體 Φ 外細胞的培養、或為組織成型、細胞株建立、細胞生理毒 理測試、細胞移行觀察、細胞與另一細胞或其它胞外基質 或因子間之各種研究與應用，或是當活體實驗難行時，通 常須使用一嵌入式細胞培養槽，提供幹細胞及其他各種類 型細胞體外培養之用。 目前現有的細胞培養槽設計，如第七圖所示，其主要 係包括一槽體（40)以及一膜片（5〇)，所述之槽體 (40)中形成一上下貫通狀的容置空間，並於該槽體 ❹ （4〇)底部一體成形有三支支撐腳（41)，該膜片 (50)設於該槽體（4〇)底部開口。 惟則述之細胞培養槽設計，該槽體係透過鑄模以鑄造 方式製造成形，製成後的細胞培養槽為一形狀固定、不可 變的型體，且不具有可調整性，又因轉模的成本高昂，若 須訂製規格以外的產品，通常須支付报高費用。 再者，前述細胞培養槽為腳架式構造設計，對於培養 基的流通性以及顯微觀察便利性會產生互相抑制的現象’ 200944589 、 ®為’若該培養槽腳架高度料偏低時，該細胞培養槽浸 置液態培養基中，會因膜片高度過低，造成培養液與添加 劑等於膜片内外的通透性不佳，不利於細胞培養。 、若該培養槽腳架高度設計偏高時，雖無前述培養基通 透性不佳之問題，但是，當使用者欲進行活細胞觀察或影 像拍攝時，如要做高倍率的顯微影像觀察時，都需使用高 倍物鏡時，其高倍鏡頭ΝΑ值大，工作距離短，此時因培 養槽的腳架過高，且該細胞培養槽不具備升降調整功能， ® 造成調整焦距的困難，以致取得的影像不理想。 【發明内容】 本發明之主要目的在於提供一種嵌入式培養槽，希藉 此叹叶，解決先前既有細胞培養槽不具升降調整功能之缺 點。 ' 為達成前揭目的，本發明所設計的嵌入式培養槽係包 括： —腳架’其包括一環狀套接部以及多支支撐腳，該些 ® 支撐腳分佈於該套接部底面，該套接部中形成一升降空 間’且於該升降空間側壁上設有第一調整部； 中空本體’該本體外周面上設有一對應於該第一調 整部的第二調整部，使本體可活動地裝設於該腳架套接部 之升降空間内，並藉該第一、第二調整部之組合而能升降 調整定位；以及 —膜片’其係固設於該本體底端呈緊繃平整狀。 本發明藉由前述嵌入式培養槽設計，其特點至少包括 200944589 有： .具有升降調整定位力能· 基诚技μ ^ 力月b .本發明所設計的嵌入式培 養槽，係利用其本體與腳架間第_ 合，使該底面設有膜片的本體可於調整之搭配組 $犋片的本體可於該腳架中升降立 置0 2. 易製造、成本低：本發明所設計的嵌人式培養槽中 所使用腳架、本體等構件，因其形狀構造簡單’且可使用 南分子聚合物等塑膠材料透過一般機械加工機具以車 ❹削、…等加工方式加以製造成形，不需訂製專用的成形模 具，具有易製造、成本低等功用。 3. 規格具有良好的可變性，便於客製化：本發明所設 汁的嵌入式培養槽之主體設計，可使用一般機械加工機具 機製成形，故能因應客戶的使用需求，改變該產品主體的 規格尺寸，並能加以實現，且產品的基本製造需求量可大 可小無限制，均能讓使用者不必支付高額款項即能取得所 需的產品，具有良好的生產經濟性。 ❿ 4.使培養液等具有良好的通透性：本發明所設計的培 養槽’當其應用於細胞培養時，底面設有膜片的本體可藉 由腳架予以架高，使該培養液與添加劑、…等培養基於膜 片處可以獲得良好的通透性。 5.顯微鏡觀察無焦距難調之顧慮：本發明所設計的嵌 入式培養槽的主體（包括本鱧及腳架等）因具有可升降之調 整功能，故於該培養槽於培養細胞的中途或完成時，使用 者欲進行細胞的顯微觀察時，可將該嵌入式培養槽放置於 6 200944589 顯微鏡下進行’其中除可以顯微鏡本身的調整機構進行對 焦外，也可透過該培養槽本身可升降調整的機制，使設於 本體底端的膜片可以接近於該顯微鏡之物鏡，辅助顯微鏡 對焦’以利使用者操作顯微鏡觀察該培養槽中的細胞生長 情況或擷取影像。 【實施方式】 如第一、五圖所示，係揭示本發明嵌入式培養槽之數 種具體可行的較佳實施例，由圖中可以見及，所述之嵌入 ❼式培養槽包括一腳架（10)、一本體（20)以及一膜 片（3 0 )，其中： 所述的腳架（1 〇 )包括一環狀套接部（i i )以及 多支支撐腳（12)，該些支撐腳（12)分佈於該套接 部（11)底面朝下延伸，該套接部（11)中形成一升 降活動空間（1 4 )，以及位於該升降活動空間（丄4 ) 側壁上的第一調整部（1 3 )。 所述的本體（20)可為一空心的管狀體，並可升降 ® 地組設於該腳架（10)的套接部（]_]_)中，該本體 (20)外周面上設有一對應於第一調整部（1 3)的第 二調整部（21)，藉由該第一調整部（13)、第二調 整部（21)之對應配合，而使本體（2〇)可於該腳架 (1 0 )中升降定位。 所述的媒片（3 0 )可為多孔性耐儉（nyl〇n)等材 料所製成之相，其臈厚 >約，孔洞大小依兩 要選用，該膜片（3 0)可藉由熱塵合手段或其他固接: 200944589 段固設於該本體（20)底端，且該膜片（30)設於本 體（2 0 )底端呈緊繃平整狀態，用以提供活體細胞附著 其表面成長，及以便於顯微觀察。 如第一圖所示之第一較佳實施例’該腳架（1 〇)設 於套接部（11)内側壁上的第一調整部（13)係為凸 粒鼓计’ fet於本體（2 0 )外周面之第二調整部（2 1 ) 可為内凹之導槽設計，所述導槽具有一縱向槽溝段（2 1 1 )以及一銜接於該縱向槽溝段（2 i 1 )底端的橫向槽 溝奴（212)而形成l形之溝槽，提供該凸粒狀第一調 整部（1 3)沿著該導槽狀第二調整部（2 i )移動，而 使本體（2 0)可於該腳架（1〇)中升降調整定位如 第二至四圖所示者。 φ 如第五、/、圖所示之第二較佳實施例，該腳架（1 〇 )設於套接部（1 1 )内側壁上的第-調整部（i 3 ) ^為内螺紋之設計；設於本體（2 Q )外周面之第二調整 4 ( 2 1 )為對應該内螺紋之外螺紋設計’藉此使該本體 (2 0)螺接於該腳架（1 〇)的套接部（1 i )中，並 可利用旋轉方式使本體（20)於該腳架（1 調整定位。 _本發心前揭具有升降調整定位功能之嵌人式培養槽 十T使用時，該培養槽可浸置包含有培養液及添加 ' 之培養基中，另將待培養的活體細胞放在該通透 以平的多孔㈣片表面，使細胞存活於培養基中 ° ; σ養日夺’使用者可令該培養槽中設於腳架上的本 8 200944589 ㈣高位置’使培養液與添加劑於膜片中具有通透 性，使膜片表面的細胞可受到良好的培養。當細胞培養中 途或完成時，欲透過顯微鏡進行觀察時，可令培養槽中的 本體下降，使膜片可以較接近顯微鏡的物n㈣㈣ 微鏡的焦距’使取得的影像較為理想。 綜上所述，本發明ϋ由該具有彳降調整功㈣欲入式 培養槽設計，確可克服先前既有之嵌入式細胞培養槽於顯 微鏡下觀察時調整焦距不易的問題，並以簡單易製造的構 ❿造設計，使其降低製造成本，兼具易於操作使用，是故， 本發明於細胞培養技術領域、或其他生物材料的顯微觀察 等技術領域中，確為-項極具產業利用價值的發明。 【圖式簡單說明】 第一圖係本發明嵌入式培養槽之第一較佳實施例之立 體分解示意圖。 第二、三圖係第一圖所示嵌入式培養槽第一較佳實施 例之升降調整本體高度之立體示意圖。 © 帛四圖係第-圖所示喪人式培養槽第—較佳實施例降 低本體高度後的剖面示意圖。 第五圖係本發明嵌入式培養槽之第二較佳實施例之立 體分解示意圖。 第六圖係第五圖所示嵌入式培養槽第二較佳實施例的 剖面示意圖。 第七圖係習知嵌入式細胞培養槽之組合剖面示意圖。 【主要元件符號說明】 200944589 (1 0 )腳架 （1 (1 2 )支撐腳 （1 (1 4 )升降空間 (2 0 )本體 （2 (2 1 1 )縱向槽溝段（2 (3 0 )膜片 (4 0 )槽體 （4 (5 0 )膜片 1 )套接部 3 )第一調整部 1 )第二調整部 1 2 )橫向槽溝段 1 )支撐腳200944589 IX. DESCRIPTION OF THE INVENTION: TECHNICAL FIELD The present invention relates to an embedded culture tank, and more particularly to a cell culture apparatus which can be applied to the field of living cell culture, or used as a microscopic observation field of biological materials. The vehicle is designed to facilitate microscopic observation of live cells or other biological materials and to record the embedded culture tank design. [Prior Art] For the research and application of cell biology, for the purpose of culturing cells outside the body Φ, or for tissue formation, cell line establishment, cell physiology and toxicity testing, cell migration observation, cells and another cell Or in the study and application of other extracellular matrices or factors, or when it is difficult to perform in vivo experiments, an embedded cell culture tank is usually used to provide stem cells and other various types of cells for in vitro culture. At present, the existing cell culture tank design, as shown in the seventh figure, mainly includes a tank body (40) and a diaphragm (5 〇), and the tank body (40) forms a vertically penetrating capacity. A space is provided, and three support legs (41) are integrally formed on the bottom of the groove body (4〇), and the diaphragm (50) is disposed at the bottom opening of the groove body (4〇). However, the cell culture tank is designed, and the tank system is formed by casting by casting. The prepared cell culture tank is a fixed shape and immutable shape, and has no adjustability, and is also modified by the mold. The cost is high, and if you want to order a product other than the specifications, you usually have to pay the high fee. Furthermore, the cell culture tank is designed as a tripod structure, and the flowability of the medium and the convenience of microscopic observation are mutually inhibited. '200944589, ® is 'If the height of the culture trough is low, When the cell culture tank is immersed in the liquid medium, the height of the membrane is too low, and the culture solution and the additive are equal to the permeability inside and outside the membrane, which is not favorable for cell culture. If the height of the culture trough is too high, although there is no problem of poor permeability of the above-mentioned medium, when the user wants to observe or image the living cells, if high-magnification microscopic images are to be observed. When a high-power objective lens is used, the high-power lens has a large value and a short working distance. At this time, because the tripod of the culture tank is too high, and the cell culture tank does not have the lifting adjustment function, the difficulty of adjusting the focal length is obtained. The image is not ideal. SUMMARY OF THE INVENTION The main object of the present invention is to provide an embedded culture tank, which is used to solve the disadvantage that the prior cell culture tank does not have a lifting adjustment function. In order to achieve the foregoing, the embedded culture tank designed by the present invention comprises: a tripod 'including an annular sleeve portion and a plurality of support legs, and the support feet are distributed on the bottom surface of the sleeve portion. a lifting space is formed in the socket portion, and a first adjusting portion is disposed on the sidewall of the lifting space; the hollow body is provided with a second adjusting portion corresponding to the first adjusting portion on the outer peripheral surface of the hollow body, so that the body can be The movable device is installed in the lifting space of the tripod socket, and can be adjusted up and down by the combination of the first and second adjusting portions; and the diaphragm is fixed at the bottom end of the body. Stretched and finished. The invention adopts the above-mentioned embedded culture tank design, and its characteristics include at least 200944589. There is a lifting and adjusting positioning force energy, and the embedded culture tank designed by the invention utilizes the body and the body. The body of the tripod is _, so that the body with the diaphragm on the bottom surface can be adjusted to match the group. The body of the cymbal can be lifted and lowered in the tripod. 0 2. Easy to manufacture, low cost: designed by the present invention The components such as the stand and the body used in the embedded culture tank are simple in shape and can be manufactured by using a plastic material such as a southern molecular polymer through a general machining tool, such as boring, etc. Custom-made forming molds are required, which are easy to manufacture and low in cost. 3. The specification has good variability and is easy to customize: the main body design of the embedded culture tank provided with the juice of the invention can be formed by using a general mechanical processing mechanism mechanism, so that the main body of the product can be changed according to the customer's use requirements. The size and size of the product can be realized, and the basic manufacturing requirements of the product can be large or small, and the user can obtain the required product without paying a large amount of money, and has good production economy. ❿ 4. The culture solution and the like have good permeability: the culture tank designed by the present invention is used when the cell culture is applied, and the body having the membrane on the bottom surface can be elevated by a tripod to make the culture solution Good permeability can be obtained at the membrane with an additive, etc. medium. 5. Microscopic observation of the problem that the focal length is difficult to adjust: the main body of the embedded culture tank designed by the present invention (including the sputum and the tripod, etc.) has a function of lifting and lowering, so that the culture tank is in the middle of the cultured cells or When the user wants to perform microscopic observation of the cells, the embedded culture tank can be placed under the microscope of 6 200944589. In addition, the adjustment mechanism of the microscope itself can be used for focusing, and the culture tank can be lifted and lowered by itself. The adjustment mechanism is such that the diaphragm disposed at the bottom end of the body can be close to the objective lens of the microscope, and the auxiliary microscope is focused to facilitate the user to observe the growth of the cells in the culture tank or to capture images. [Embodiment] As shown in the first and fifth figures, several specific and feasible embodiments of the embedded culture tank of the present invention are disclosed. As can be seen from the figure, the embedded cultivating tank includes a foot. a frame (10), a body (20) and a diaphragm (30), wherein: the tripod (1) includes an annular socket (ii) and a plurality of support legs (12), The supporting legs (12) are distributed downwardly on the bottom surface of the socket portion (11), and a lifting and lowering movable space (14) is formed in the socket portion (11), and is located on the side wall of the lifting and lowering activity space (丄4) The first adjustment section (13). The body (20) can be a hollow tubular body, and can be assembled and lowered in the sleeve portion (]_]_) of the stand (10), and the outer peripheral surface of the body (20) is disposed. There is a second adjusting portion (21) corresponding to the first adjusting portion (13), and the main adjusting portion (13) and the second adjusting portion (21) are matched to each other, so that the body (2〇) can be Lifting and positioning in the tripod (10). The medium (30) may be a phase made of a material such as porous yttrium (nyl〇n), and the thickness thereof is about ≥, and the size of the hole is selected according to two, and the film (30) may be used. By means of thermal dusting or other fixing: 200944589 is fixed at the bottom end of the body (20), and the diaphragm (30) is arranged at the bottom end of the body (20) in a tight and flat state for providing a living body. The cells adhere to their surface growth and facilitate microscopic observation. As shown in the first figure, the first adjustment portion (13) of the tripod (1 设) provided on the inner side wall of the socket portion (11) is a convex drum meter 'fet on the body (20) The second adjustment portion (2 1 ) of the outer circumferential surface may be a concave guide groove, the guide groove has a longitudinal groove segment (2 1 1 ) and a longitudinal groove segment (2) i 1) the lateral groove of the bottom end (212) forms an l-shaped groove, and provides the convex first adjustment portion (13) to move along the channel-shaped second adjustment portion (2 i ), The body (20) can be lifted and adjusted in the stand (1〇) to be positioned as shown in the second to fourth figures. φ As shown in the fifth and/or the second preferred embodiment, the first adjustment portion (i 3 ) of the tripod (1 〇) provided on the inner side wall of the socket portion (1 1 ) is internally threaded. The second adjustment 4 ( 2 1 ) provided on the outer peripheral surface of the body (2 Q ) is designed to correspond to the external thread of the internal thread, thereby screwing the body (20) to the stand (1 〇) In the socket portion (1 i ), the body (20) can be rotated on the tripod (1). The ergonomic culture tank with the lifting adjustment positioning function is used before the tenth. The culture tank can be immersed in the medium containing the culture solution and the added medium, and the living cells to be cultured are placed on the surface of the transparent porous plate to allow the cells to survive in the culture medium; 'The user can make the culture medium and the high position of the medium on the tripod to make the culture solution and the additive have permeability in the membrane, so that the cells on the surface of the membrane can be well cultured. When the culture is in progress or completed, when the microscope is to be observed, the body in the culture tank can be lowered, so that the membrane can be connected. The near-microscope object n(4)(4) The focal length of the micromirror makes the obtained image ideal. In summary, the invention has the design of the immersion adjustment device (4), which can overcome the existing embedded cells. When the culture tank is observed under a microscope, the problem of difficulty in adjusting the focal length is difficult, and the design is simple and easy to manufacture, so that the manufacturing cost is reduced, and the operation is easy to use. Therefore, the present invention is in the field of cell culture technology or other organisms. In the technical field of microscopic observation of materials, it is indeed an invention with industrial value. [First Description of the Drawings] The first figure is a perspective exploded view of the first preferred embodiment of the embedded culture tank of the present invention. The second and third figures are perspective views of the height of the lifting and adjusting body of the first preferred embodiment of the embedded culture tank shown in the first figure. © Figure 4 - Figure 10 The cross-sectional view of the second preferred embodiment of the embedded culture tank of the present invention is shown in the fifth figure. A cross-sectional view of a second preferred embodiment of the culture tank. The seventh diagram is a schematic cross-sectional view of a conventional embedded cell culture tank. [Key element symbol description] 200944589 (1 0 ) Tripod (1 (1 2 ) support foot ( 1 (1 4 ) lifting space (20) body (2 (2 1 1 ) longitudinal groove section (2 (3 0) diaphragm (4 0) groove body (4 (5 0) diaphragm 1) socket 3) First adjustment part 1) Second adjustment part 1 2) Lateral groove section 1) Supporting foot
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