CN210215396U - Cell co-culture device - Google Patents
Cell co-culture device Download PDFInfo
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- CN210215396U CN210215396U CN201920789497.8U CN201920789497U CN210215396U CN 210215396 U CN210215396 U CN 210215396U CN 201920789497 U CN201920789497 U CN 201920789497U CN 210215396 U CN210215396 U CN 210215396U
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- 238000003501 co-culture Methods 0.000 title claims abstract description 26
- 230000000694 effects Effects 0.000 claims abstract description 4
- 239000012528 membrane Substances 0.000 claims description 14
- 229920006289 polycarbonate film Polymers 0.000 claims description 6
- 239000011148 porous material Substances 0.000 claims description 4
- 229920000515 polycarbonate Polymers 0.000 claims 1
- 239000004417 polycarbonate Substances 0.000 claims 1
- 238000011160 research Methods 0.000 abstract description 11
- 230000010261 cell growth Effects 0.000 abstract description 6
- 230000008614 cellular interaction Effects 0.000 abstract description 4
- 230000003993 interaction Effects 0.000 description 7
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 5
- 238000004113 cell culture Methods 0.000 description 5
- 235000015097 nutrients Nutrition 0.000 description 5
- 239000001301 oxygen Substances 0.000 description 5
- 229910052760 oxygen Inorganic materials 0.000 description 5
- 102000004127 Cytokines Human genes 0.000 description 4
- 108090000695 Cytokines Proteins 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 4
- 230000019522 cellular metabolic process Effects 0.000 description 4
- 238000009792 diffusion process Methods 0.000 description 4
- 230000035515 penetration Effects 0.000 description 4
- 238000001727 in vivo Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a cell co-culture device, including the culture dish, be provided with assorted ware lid on the culture dish, ware lid below is provided with dead lever one, and dead lever one is gone up the activity and is provided with adjustable dead lever two, and two lower extremes of dead lever are provided with a plurality of connecting rods, and the connecting rod other end all is provided with cultivates the room, is provided with a plurality of diaphragms in the culture dish. The utility model discloses convenient to use simulates real cell growth environment, can once carry out the cultivation of many copies of cells or carry out the research of a plurality of cell interactions, will cultivate the room unsettled, replaces the cell support frame, and unsettled height can carry out the regulation of a certain degree.
Description
Technical Field
The utility model belongs to the technical field of cell culture equipment, concretely relates to cell co-culture device.
Background
In order to establish a culture system more similar to the in vivo environment and to make the in vitro environment and the in vivo environment coincide as much as possible, so that the cells can communicate information with each other and support growth and proliferation mutually, a cell co-culture technology is developed on the basis of the cell culture technology. The cell co-culture technique is to co-culture 2 or more cells in the same environment, and is widely applied to modern cell research due to the advantage of better reflecting the in vivo environment.
The cell co-culture system is divided into a direct co-culture system and an indirect co-culture system, wherein the indirect co-culture system is that 2 or more than 2 cells are respectively inoculated on different carriers, then the different carriers are placed in the same culture environment, so that the different cells are co-located in the same culture system without direct contact, and the different cells can be respectively collected for the next research. The existing co-culture device has a simple structure, can not culture multiple cells or multiple cells at one time, or simultaneously researches the interaction among the multiple cells, and also adopts a cell support frame to support a culture chamber, occupies the space of a culture dish, has fixed support frame height and can not be adjusted to a certain extent.
SUMMERY OF THE UTILITY MODEL
To the above-mentioned not enough, the utility model provides a cell co-culture device, convenient to use simulates real cell growth environment, can once carry out the cultivation of many copies cell or carry out the research of a plurality of cell interactions, will cultivate the room unsettled, replaces the cell support frame, and unsettled height can carry out the regulation of a certain degree.
In order to achieve the above object, the present invention provides a technical solution for solving the technical problem: the utility model provides a cell co-culture device, including the culture dish, be provided with assorted ware lid on the culture dish, ware lid below is provided with dead lever one, and the activity is provided with adjustable cavity dead lever two on the dead lever one, and two lower extremes of dead lever are provided with a plurality of connecting rods, and the connecting rod other end all is provided with cultivates the room, is provided with a plurality of diaphragms in the culture dish.
The utility model has the advantages that: when cell co-culture is carried out, if multiple cultures of the same cell are needed, multiple cells are inoculated into multiple culture chambers, then the dish cover is closed, the culture chambers enter culture solution contained in a culture dish, the culture solution can be communicated through a diaphragm, the multiple cells can be cultured at one time, the culture conditions are the same, the obtained cells are basically the same, and the cell co-culture method can be used for subsequent cell research; if the interaction of various cells is to be researched, the various cells are respectively inoculated in the corresponding culture chambers, then the culture chambers are placed in culture solution of a culture dish, the dish cover is closed, and in the cell culture process, the membrane can block the diffusion of the cells, but does not block the penetration of cytokines, nutrients, oxygen and the like generated by cell metabolism, so that the mutual exchange and the co-action of cell metabolites are realized, and the interaction of the various cells is researched; the utility model discloses a dead lever one, dead lever two and connecting rod will cultivate the unsettled setting of room, replace the cell support frame, maneuverability is stronger, can realize the multiple combined culture of multicellular, and dead lever one and two swing joint of dead lever also can suitably adjust the distance of two poles, change the height of cultivateing the room, realize the appropriate regulation to cultivateing the room height.
Further, the separator may be a polycarbonate film.
The beneficial effect of adopting the further scheme is that: the polycarbonate film is used for blocking the diffusion of cells, but does not block the penetration of cytokines, nutrients, oxygen and the like generated by cell metabolism, so that the real cell growth environment is simulated, and the culture of various cells and the research of multi-cell interaction are realized.
Further, the pore diameter of the polycarbonate film is not more than 0.3. mu.m.
Further, the membrane may be a permeable membrane.
The beneficial effect of adopting the further scheme is that: the permeable membrane can divide the culture dish into a plurality of partition areas, but does not influence the free permeability of chemical substances, avoids direct contact among cells, and can conveniently observe the living states of various cells or microorganisms.
Furthermore, the aperture of the permeable membrane is 20-50 μm.
Furthermore, the first fixing rod and the second fixing rod are in threaded connection.
The beneficial effect of adopting the further scheme is that: threaded connection makes the connection more firm, the dismouting of also being convenient for, and is simple and convenient, can suitably adjust the distance of dead lever one and dead lever two simultaneously through the screw thread, realizes the regulation of cultivateing the room height.
Furthermore, the bottom of the culture dish is provided with a fixed groove corresponding to the culture chamber.
The beneficial effect of adopting the further scheme is that: the fixed slot can make the culture room fixed more firm, builds more stable growing environment.
Drawings
FIG. 1 is a schematic view of the present invention;
FIG. 2 is a top view of a culture dish;
wherein, 1, a culture dish; 2. a dish cover; 3. fixing a rod I; 4. a second fixing rod; 5. a connecting rod; 6. a culture chamber; 7. a diaphragm.
Detailed Description
The following detailed description of the embodiments of the present invention will be made with reference to the accompanying drawings.
The utility model discloses an embodiment, as shown in fig. 1-2, provides a cell co-culture device, including culture dish 1, be provided with assorted dish lid 2 on the culture dish 1, be provided with dead lever one 3 below the dish lid 2, dead lever one 3 go up the activity and is provided with adjustable cavity dead lever two 4, and dead lever two 4 lower extreme is provided with a plurality of connecting rods 5, and the connecting rod 5 other end all is provided with culture chamber 6, is provided with a plurality of diaphragms 7 in the culture dish 1. When cell co-culture is carried out, if multiple cultures of the same cell are needed, multiple cells are inoculated into multiple culture chambers 6, then the dish cover 2 is closed, the culture chambers 6 enter culture solution contained in the culture dish 1, the culture solution can be communicated through the diaphragm 7, multiple cells can be cultured at one time, the culture conditions are the same, the obtained cells are basically the same, and the cell co-culture can be used for subsequent cell research; if the interaction of various cells is to be researched, the various cells are respectively inoculated in the corresponding culture chambers 6, then the culture chambers 6 are placed in the culture solution of the culture dish 1, the dish cover 2 is closed, and in the cell culture process, the membrane 7 can block the diffusion of the cells, but does not block the penetration of cytokines, nutrient components, oxygen and the like generated by cell metabolism, so that the mutual exchange and the mutual action of cell metabolites are realized, and the interaction among the cells is researched; the utility model discloses a dead lever 3, dead lever two 4 and connecting rod 5 will cultivate 6 unsettled settings in room, replace the cell support frame, maneuverability is stronger, can realize the multiple combination culture of multicellular, dead lever 3 and two 4 swing joints of dead lever, also can suitably adjust the distance of two poles, change the height of cultivateing room 6, realize the appropriate regulation to cultivateing 6 heights in room.
The diaphragm 7 can be a polycarbonate film, the pore diameter is not more than 0.3 μm, the diffusion of cells is blocked by the polycarbonate film, but the penetration of cytokines, nutrient components, oxygen and the like generated by cell metabolism is not hindered, the real cell growth environment is simulated, and the culture of various cells and the research of multi-cell interaction are realized. The membrane 7 can be a permeable membrane with the aperture of 20-50 μm, and the culture dish 1 can be divided into a plurality of separated areas by the permeable membrane, but the free permeability of chemical substances is not influenced, the direct contact between cells is avoided, and the living state of various cells or microorganisms can be conveniently observed. The first fixed rod 3 is in threaded connection with the second fixed rod 4, the threaded connection enables the connection to be more stable, the disassembly and the assembly are convenient, simplicity and convenience are achieved, the distance between the first fixed rod 3 and the second fixed rod 4 can be properly adjusted through threads, and the height of the culture chamber 6 is adjusted. The bottom of the culture dish 1 is provided with a fixing groove corresponding to the culture chamber 6, and the fixing groove can ensure that the culture chamber 6 is fixed more firmly, so that a more stable growth environment is created.
When the culture dish is used, multiple cultures or multiple cell cultures of the same cell are carried out, the cell is inoculated into a plurality of culture chambers 6, then the dish cover 2 is closed, the culture chambers 6 enter culture solution contained in the culture dish 1, nutrient substances, oxygen and the like in the culture solution can be communicated through the diaphragm 7, a real cell growth environment is simulated, and the research on the multi-chamber culture or the multiple cell interaction of the cell is carried out. The utility model discloses convenient to use simulates real cell growth environment, can once carry out the cultivation of many copies of cells or carry out the research of a plurality of cell interactions, will cultivate room 6 unsettled, replaces the cell support frame, and unsettled height can carry out the regulation of a certain degree.
While the present invention has been described in detail and with reference to the accompanying drawings, it is not to be considered as limited to the scope of the invention. Various modifications and changes may be made by those skilled in the art without inventive step within the scope of the appended claims.
Claims (7)
1. The utility model provides a cell co-culture device, its characterized in that, includes culture dish (1), be provided with assorted dish lid (2) on culture dish (1), dish lid (2) below is provided with dead lever (3), the activity is provided with adjustable cavity dead lever two (4) on dead lever one (3), dead lever two (4) lower extreme is provided with a plurality of connecting rods (5), the connecting rod (5) other end all is provided with cultivates room (6), be provided with a plurality of diaphragms (7) in culture dish (1).
2. Cell co-culture device according to claim 1, characterized in that the membrane (7) can be a polycarbonate film.
3. The cell co-culture apparatus of claim 2, wherein the polycarbonate membrane has a pore size of not greater than 0.3 μm.
4. Cell co-culture device according to claim 1, wherein the membrane (7) is a permeable membrane.
5. The cell co-culture apparatus of claim 4, wherein the pore size of the permeable membrane is 20-50 μm.
6. The cell co-culture apparatus of claim 1, wherein the fixing rod one (3) and the fixing rod two (4) are screw-coupled.
7. The cell co-culture apparatus according to claim 1, wherein the bottom of the culture dish (1) is provided with fixing grooves corresponding to the culture chambers (6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920789497.8U CN210215396U (en) | 2019-05-28 | 2019-05-28 | Cell co-culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920789497.8U CN210215396U (en) | 2019-05-28 | 2019-05-28 | Cell co-culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210215396U true CN210215396U (en) | 2020-03-31 |
Family
ID=69928335
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920789497.8U Active CN210215396U (en) | 2019-05-28 | 2019-05-28 | Cell co-culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210215396U (en) |
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2019
- 2019-05-28 CN CN201920789497.8U patent/CN210215396U/en active Active
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20240709 Address after: No. 1666, Section 2, Chenglong Avenue, Chengdu Economic and Technological Development Zone (Longquanyi District), Chengdu City, Sichuan Province, 610100, China Patentee after: Aorui Saisi Biotechnology (Chengdu) Co.,Ltd. Country or region after: China Address before: 610106 Chengdu University of waidong mausoleum, Chengdu, Sichuan Patentee before: CHENGDU University Country or region before: China |