CN207264658U - Simulating plant root, Rhizosphere ecosystem simulator - Google Patents
Simulating plant root, Rhizosphere ecosystem simulator Download PDFInfo
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- CN207264658U CN207264658U CN201720602562.2U CN201720602562U CN207264658U CN 207264658 U CN207264658 U CN 207264658U CN 201720602562 U CN201720602562 U CN 201720602562U CN 207264658 U CN207264658 U CN 207264658U
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- plant root
- warehouse
- simulating plant
- storehouse
- injection port
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Abstract
The utility model discloses a kind of plant organ model configuration and experimental rig.For existing product there are the defects of of high cost, sample generation deficiency, collection difficulty, the utility model provides a kind of simulating plant root and Rhizosphere ecosystem simulator.Simulating plant root is elongated tubular construction, and leading portion is infiltration section, and the pipe fitting that the front end being processed into by porous, hydrophilic filter membrane is closed, stage casing is extension tube, is that PE is managed or pvc pipe, latter end are injection ports.There is stainless steel steel wire inside elongated tubular construction.Rhizosphere ecosystem simulator includes simulating plant root, cultivates storehouse, into exemplar, injection port plug, simulating plant root is stretched into through the location hole for cultivating top bar on the warehouse of storehouse and cultivated in storehouse, injection port is being cultivated outside storehouse, is separate piece into exemplar, injection port plug, and cultivating storehouse can open.This simulating plant root architecture is succinct and can simulate root secreting function.Rhizosphere ecosystem simulator energy high effect culture Rhizosphere Soil sample and sample collection convenience.Product structure is simple, processing cost is low, property easy to use is high.
Description
Technical field
A kind of plant organ model configuration and device are the utility model is related to, more particularly to a kind of simulating plant root and one
Kind Rhizosphere ecosystem simulator, belongs to experiment machinery field.
Background technology
Root is the important nutrition organs of plant, while being responsible for absorbing the moisture and inorganic salts inside soil, also to soil
An earth secretion part comes from photosynthetic product (i.e. root exudates).Its Function is plant life structure and process
Basis, and the basic research field of the subject such as botany, agrology, ecology.1904, German microbiologist
Lorenz Hiltner propose rhizosphere concept, the soil body that rhizosphere is defined as around root system, is influenced by root growth by he.Hereafter,
Correlative study gradually discloses, and rhizosphere is that soil-plant carries out mass exchange and enlivens interface.Plant is assimilated big by photosynthesis
CO in gas2, part photosynthate is discharged into rhizosphere by root system, excites the metabolism of edaphon；And soil is micro-
It is biological then organic nutrient is changed into inorganic forms by the use of these photosynthates as energy substance, in favor of plant absorption profit
With.Many electrochemical conditions of rhizosphere and Biochemical processes are different from soil body soil, and most obvious one is exactly pH in Rhizosphere, oxygen
Change change of reduction potential and microbial activity etc..Although rhizosphere soil account for soil cumulative volume ratio it is relatively minimum (generally away from
Within several millimeters from root axis surface), but rhizosphere process is the important component of Terrestrial Carbon and Nutrient Cycling, and rhizosphere is land
The important content of lower ecological process research.But around root system development field research, there are many difficulties.For example, it is limited to exceptionally
Complicated wild environment, collection of the researcher to Rhizosphere Soil sample are not ideal enough；In addition, if to probe into one-component or difference
When " rhizosphere " of the induction of Chemical Measurement root exudates, wild environment is even more that can not meet such condition.Therefore, using people
Work structural simulation, designs a blanket simulating plant root architecture and rhizosphere simulator and seems extremely necessary.
A kind of rhizosphere simulator (Keiluweit et al.2015) is disclosed in the prior art, and the device is using wriggling
Root exudates solution is pumped into rhizosphere box by pump, and a simulation root is included in rhizosphere box.The device when in use exist with
Lower 3 point defects：(1) of high cost, peristaltic pump is expensive；(2) there was only a simulation root, its long-living Rhizosphere Soil seriously cannot
Meet testing requirement；(3) it is not easy enough when uneven situation can be caused to occur and operate when adding root exudates.
Keiluweit et al.(2015).Mineral protection of soil carbon counteracted
by root exudates.Nature Climate Change 5(6):588-595.
Utility model content
The purpose of this utility model is aiming at the deficiencies in the prior art, there is provided a kind of simulating plant root is given birth to a kind of rhizosphere
State simulator.The product being capable of effectively simulating plant root secreting function and Rhizosphere ecosystem environment, and processing cost is low, user
Just.
To achieve the above object, the utility model provides a kind of simulating plant root first, its technical solution is as follows：
A kind of simulating plant root, it is characterised in that：It is elongated tubular construction；The elongated tubular construction leading portion is infiltration
Section, the pipe fitting that the front end being processed into by porous, hydrophilic filter membrane is closed, stage casing is extension tube, is PE pipes or pvc pipe, latter end be into
Sample mouth.
Above-mentioned simulating plant root is integrally a kind of elongated tubular construction of front end closing, effectively simulates the exterior shape of root
State and overall structure.Elongated tubular construction be divided into before, in, end three sections.Leading portion is infiltration section, is processed using porous, hydrophilic filter membrane
Into simulating the tip of a root of root, can complete to secrete the function of organic substance into soil.Stage casing extension tube is managed using PE or pvc pipe,
The guide-tube structure of root is simulated, realizes material conveying function.Latter end injection port is used for the injection simulation root system point into simulating plant root
Secretion.
Further, permeate between section, extension tube, injection port and be bonded.Alternatively, glued between infiltration section and extension tube by connecting tube
Connect, connecting tube be PE pipe or pvc pipe, it is be bonded between extension tube and injection port.
Further, there is stainless steel steel wire inside elongated tubular construction, stainless steel steel wire length is more than infiltration segment length, small
In infiltration segment length with extending the sum of length of tube.Stainless steel function of wire is to provide mechanical support, prevents elongated tubular bending, and just
Just hold and simulating plant root is inserted into soil when extending pipeline section.
Under optimum condition, the specification of above-mentioned simulating plant root is：Permeate 0.13 μm~0.18 μm of section average pore size.The ginseng
Number design is for the effectively epidermal structure of simulation root and prevents microorganism to enter in root at the same time.
For above-mentioned simulating plant root is effectively applied to plant rhizosphere research, the utility model, which further provides for one kind, to be made
The Rhizosphere ecosystem simulator realized with above-mentioned simulating plant root, its technical solution are as follows：
A kind of Rhizosphere ecosystem simulator realized using above-mentioned simulating plant root, it is characterised in that：Including simulating plant
Root, cultivate storehouse, into exemplar, injection port plug；The cultivation storehouse includes warehouse and cover board, and warehouse is detachably to couple with cover board；Institute
State simulating plant root to stretch into cultivation storehouse through the location hole for cultivating top bar on the warehouse of storehouse, injection port is being cultivated outside storehouse；It is described into
Exemplar, injection port plug are separate pieces.
Above-mentioned Rhizosphere ecosystem simulator further includes in addition to the utility model product simulation plant roots and cultivates storehouse and sample introduction
Part.It can be hexahedron empty in one, the infiltration section of simulating plant root and the part of extension tube from cultivation the top of the warehouse to cultivate storehouse
Perforate stretch into space in storehouse, injection port, which is retained in, to be cultivated outside storehouse.For ease of the filling soil in storehouse is cultivated, cultivate storehouse and be designed to
Warehouse is capped the structure of plate.Dismantle lower cover can in storehouse is cultivated filling soil.It is separate piece into exemplar, during use and sample introduction
Mouth connection, its effect is the sample introduction into simulating plant root, and sample introduction finishes, and covers injection port plug.
Further, above-mentioned Rhizosphere ecosystem simulator, two side slat of warehouse and/or lower sill strip are provided with venthole.Usually,
Venthole aperture 3mm.Can loosely filled glass fiber after filling soil in storehouse is cultivated, in venthole, it is allowed to micro- in soil
The gas and outside air that biological metabolism produces swap.
Further, above-mentioned Rhizosphere ecosystem simulator, has fixing piece on warehouse inner bottom surface, simulating plant root passes through fixation
Part is fixed on warehouse inner bottom surface, and it is detachable to push up bar on warehouse.Usually fixing piece is fastener, facilitates the installation of simulating plant root
With dismounting.The detachable draw bail of bar is pushed up on warehouse conveniently disposably to be removed simulating plant root from device, or
Installation.
Further, above-mentioned Rhizosphere ecosystem simulator, two side slat of warehouse are that acrylic material is processed into.Acrylic material
Material chemical stability is preferable, is not easy to react with soil.
Under normal circumstances, above-mentioned Rhizosphere ecosystem simulator, injection port are female Luer connectors, are syringes into exemplar.
Female Luer connector is Common parts and standard component with syringe, and conformability is high, is conveniently easy to get, of low cost.
Under optimum condition, above-mentioned Rhizosphere ecosystem simulator, cover board and warehouse inner bottom surface spacing 8mm.The spacing is set
Meter parameter is to be discharged into soil the optimised of institute's energy diffusion diameter according to root exudates and obtain.
Further, above-mentioned Rhizosphere ecosystem simulator, including at least three simulating plant roots；Permeate segment length 100mm, outside diameter
2.5mm, extends pipe range 120mm, outside diameter 2.3mm, is processed into by colourless transparent material, high 130mm in warehouse, between simulating plant root
Away from 20mm, simulating plant root and warehouse both sides inner wall spacing 20mm, location hole aperture 4mm.Under the design, device includes at least three
Simulating plant root can facilitate the present apparatus to carry out comparative studies.The design of (namely cultivating high in storehouse) high in frame is to ensure mould
Intending plant roots can stretch naturally.Simulating plant root spacing, simulating plant root and two side slat inner wall spacing of warehouse are according to root system
Secretion penetrate into soil can diffusion diameter optimised and obtain.Location hole aperture is according to the elongated of simulating plant root
The outside diameter of tubular structure determines.
Compared with prior art, the beneficial effects of the utility model are：(1) simulating plant root architecture is succinct and can simulate weight
The root secreting function wanted.Simulating plant root autgmentability is high, can be suitable for a variety of root systems, the ecological simulating apparatus that rhizosphere is studied.
(2) Rhizosphere ecosystem simulator can high effect culture go out Rhizosphere Soil sample；Installation simulation root is with gathering two, rhizosphere soil sample
Important experimental implementation is all simple easily to be realized, and effect is good.Device includes multiple simulating plant roots, development can facilitated to compare
Sufficient soil is cultivated while research.(3) pedotheque can easily be distinguished and gather rhizosphere soil and non-root in device
Border soil research material as a comparison, provides convenience to compare Rhizosphere and nonrhizosphere on ecology.(4) product structure it is simple,
Processing cost is low, property easy to use is high.
Brief description of the drawings
Fig. 1 is simulating plant root architecture schematic diagram.
Fig. 2 is the A-A cross-sectional views of Fig. 1.
Fig. 3 is Rhizosphere ecosystem simulator structure diagram.
Fig. 4 is warehouse schematical top view.
Numeral mark in attached drawing is respectively：
1 simulating plant root 11 infiltration section 12 extension tube, 13 injection port, 14 stainless steel steel wire, 15 connecting tube 2 cultivates storehouse
23 venthole of bar 212 location hole, 213 side slat, 214 times sill strips, 22 cover board, 24 fixing piece 25 is pushed up on 21 warehouses 211 to fasten
Part 3 is into 4 injection port plug of exemplar
Embodiment
Below in conjunction with the accompanying drawings, the preferred embodiment of the utility model is further described.
Embodiment one
As shown in Figure 1 and Figure 2, a kind of simulating plant root is processed.
Fig. 1 is simulating plant root architecture schematic diagram.Simulating plant root 1, is elongated tubular construction；Elongated tubular construction leading portion
It is infiltration section 11, the pipe fitting that the front end being processed into by porous, hydrophilic filter membrane is closed, stage casing is extension tube 12, is PE pipes or PVC
Pipe, latter end is injection port 13.Permeate section 11 it is be bonded by connecting tube 15 between extension tube 12, connecting tube 15 be PE pipe or pvc pipe,
It is be bonded between extension tube 12 and injection port 13.
Fig. 2 is the A-A cross-sectional views of Fig. 1.There are stainless steel steel wire 14, stainless steel steel wire inside elongated tubular construction
14 length are more than 11 length of infiltration section, less than the sum of infiltration 12 length of 11 length of section and extension tube.
In the present embodiment, 11 long 100mm of section, outside diameter 2.5mm, 0.13 μm~0.18 μm of average pore size are permeated, is extended
12 long 120mm of pipe, outside diameter 2.3mm, 15 long 7mm~8mm of connecting tube, internal diameter 2.2mm~2.3mm, outside diameter 3.6mm.Connecting tube 15
It is PE short tubes or PVC short tubes.14 long 135mm of stainless steel steel wire, diameter 1.5mm, before section 11 is permeated in the insertion of stainless steel steel wire 14
End seal closed end bottom, remainder (about 35mm) are retained in extension tube.
Embodiment two
As shown in Figure 3, Figure 4, a kind of Rhizosphere ecosystem simulator is processed, it is not repeated with one something in common of embodiment.
Fig. 3 is Rhizosphere ecosystem simulator structure diagram.Rhizosphere ecosystem simulator, including simulating plant root 1, cultivation
Storehouse 2, into exemplar 3, injection port plug 4；Cultivating storehouse 2 includes warehouse 21 and cover board 22, and warehouse 21 is detachably to couple with cover board 22；Mould
Intend plant roots 1 to stretch into cultivation storehouse 2 through the location hole 212 for cultivating top bar 211 on 2 warehouse 21 of storehouse, injection port 13 is cultivating storehouse 2
Outside；It is separate piece, injection port plug 4 into exemplar 3.Rhizosphere ecosystem simulator includes three simulating plant roots 1.21 liang of side slats of warehouse
213 and/or lower sill strip 214 be provided with venthole 23.There is fixing piece 24 on 21 inner bottom surface of warehouse, simulating plant root 1 passes through fixing piece
24 are fixed on 21 inner bottom surface of warehouse.Warehouse 21 is fixed with cover board 22 by fastener 25.
Fig. 4 is warehouse schematical top view.It is detachable that bar 211 is pushed up on warehouse 21.
In present embodiment, injection port 13 is female Luer connector, is syringe into exemplar 3.21 liang of side slats 213 of warehouse are
Acrylic material is processed into.1 spacing 20mm of simulating plant root, simulating plant root 1 and 21 both sides inner wall spacing 20mm of warehouse.Ventilation
23 aperture 3mm of hole；212 aperture 4mm of location hole.High 130mm in warehouse 21, cover board 22 and 21 inner bottom surface spacing 8mm of warehouse.It is fixed
Part 24 is fastener.
The application method of Rhizosphere ecosystem simulator is：Dismounting, which is cultivated, pushes up bar 211 and cover board 22 on 2 warehouse 21 of storehouse, by three
Simulating plant root 1 passes through location hole 212 from top to bottom, retains injection port 13 in upper 211 upside of top bar.Cultivate the simulation in storehouse 2
Plant roots fixing piece 24 is fixed.The tiling of storehouse 2 soil is being cultivated, is installing and bar 211 is pushed up on warehouse 21, make simulating plant root 1 in soil
Stretched naturally in earth, mulching soil.Or after Hatch Board 22 is cultivated in dismounting, the first filling soil in storehouse 2 is cultivated, then hand-held extension
Simulating plant root is inserted into soil at stainless steel steel wire in pipe.Two kinds of modes of operation, which all should be noted, ensures simulating plant root 1
The fully-inserted soil of leading portion in and with soil natural be in close contact.The density of the soil of loading is paid attention at the same time with field sampling
Point soil density is consistent.2 cover board 22 of storehouse is cultivated in installation, the loosely filled glass fiber in venthole 23.Experiment starts, according to
Experimental design, passes through simulating plant root with syringe by the germ-free plant root exudates solution after 0.22 μm of membrane filtration
In injection port injection soil.Rhizosphere ecosystem simulator is put into growth cabinet according to experimental design and completes culture experiment.
After culture experiment, dismounting cover board 22, upper top bar 211 and simulating plant root 1, give up and cultivate the top and bottom in storehouse
The soil of (respectively about 15mm), then cut with a knife and take native be used as of 1 surrounding 0mm~4mm of range simulation plant roots to collect Rhizosphere Soil.
The soil of 1 surrounding 5mm~15mm of simulating plant root can be used as Non-rhizosphere soil.
Claims (10)
1. simulating plant root, it is characterised in that：It is elongated tubular construction；The elongated tubular construction leading portion is infiltration section (11),
The pipe fitting that the front end being processed into by porous, hydrophilic filter membrane is closed, stage casing is extension tube (12), is that PE is managed or pvc pipe, latter end are
Injection port (13).
2. simulating plant root according to claim 1, it is characterised in that：The infiltration section (11), extension tube (12), sample introduction
It is bonded between mouth (13).
3. simulating plant root according to claim 1, it is characterised in that：Lead between the infiltration section (11) and extension tube (12)
Connecting tube (15) bonding is crossed, the connecting tube (15) is PE pipes or pvc pipe, is glued between the extension tube (12) and injection port (13)
Connect.
4. simulating plant root according to claim 1, it is characterised in that：There is stainless steel steel inside the elongated tubular construction
Silk (14), stainless steel steel wire (14) length are more than infiltration section (11) length, less than infiltration section (11) length and extension tube
(12) the sum of length.
5. the Rhizosphere ecosystem simulator realized using any simulating plant root of Claims 1 to 44, it is characterised in that：
Including simulating plant root (1), cultivate storehouse (2), into exemplar (3), injection port plug (4)；It is described cultivate storehouse (2) include warehouse (21) with
Cover board (22), warehouse (21) are detachably to couple with cover board (22)；The simulating plant root (1), which passes through, cultivates storehouse (2) warehouse
(21) location hole (212) that bar (211) is pushed up on is stretched into cultivation storehouse (2), and injection port (13) is cultivating storehouse (2) outside；The sample introduction
Part (3), injection port plug (4) are separate pieces.
6. device according to claim 5, it is characterised in that：The cover board (22) and warehouse (21) inner bottom surface spacing 8mm,
Permeate 0.13 μm~0.18 μm of section (11) average pore size.
7. device according to claim 5, it is characterised in that：(21) two side slat (213) of warehouse and/or lower sill strip (214)
It is provided with venthole (23).
8. device according to claim 7, it is characterised in that：3 mm of venthole (23) aperture, venthole (23) are interior
Loosely filled glass fiber.
9. according to any device of claim 5~8, it is characterised in that：The injection port (13) is female Luer connector,
Described into exemplar (3) is syringe, has fixing piece (24) on warehouse (21) inner bottom surface, the simulating plant root (1) passes through
Fixing piece (24) is fixed on warehouse (21) inner bottom surface, and it is detachable to push up bar (211) on the warehouse (21).
10. device according to claim 9, it is characterised in that：Including at least three simulating plant roots (1)；The infiltration section
(11) long 100mm, outside diameter 2.5mm, the long 120mm of the extension tube (12), outside diameter 2.3mm, are processed into, institute by colourless transparent material
State high 130mm in warehouse (21), simulating plant root (1) the spacing 20mm, simulating plant root (1) and warehouse (21) left and right sides
Inner wall spacing 20mm, location hole (212) the aperture 4mm, (21) two side slat (213) of warehouse is that acrylic material is processed into, institute
It is fastener to state fixing piece (24).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201720602562.2U CN207264658U (en) | 2017-05-26 | 2017-05-26 | Simulating plant root, Rhizosphere ecosystem simulator |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201720602562.2U CN207264658U (en) | 2017-05-26 | 2017-05-26 | Simulating plant root, Rhizosphere ecosystem simulator |
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| Publication Number | Publication Date |
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| CN207264658U true CN207264658U (en) | 2018-04-20 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109946223A (en) * | 2019-03-19 | 2019-06-28 | 南宁学院 | A kind of cassava pulls up the rig for model test of mechanical analysis test |
-
2017
- 2017-05-26 CN CN201720602562.2U patent/CN207264658U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109946223A (en) * | 2019-03-19 | 2019-06-28 | 南宁学院 | A kind of cassava pulls up the rig for model test of mechanical analysis test |
| CN109946223B (en) * | 2019-03-19 | 2021-09-10 | 南宁学院 | Model test stand for cassava pulling mechanical analysis test |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180420 Termination date: 20210526 |
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