CN107475093A - A kind of microorganism heating water bath culture apparatus - Google Patents
A kind of microorganism heating water bath culture apparatus Download PDFInfo
- Publication number
- CN107475093A CN107475093A CN201710556051.6A CN201710556051A CN107475093A CN 107475093 A CN107475093 A CN 107475093A CN 201710556051 A CN201710556051 A CN 201710556051A CN 107475093 A CN107475093 A CN 107475093A
- Authority
- CN
- China
- Prior art keywords
- base fluid
- water
- peristaltic pump
- blake bottle
- microorganism
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 244000005700 microbiome Species 0.000 title claims abstract description 32
- 239000008236 heating water Substances 0.000 title claims abstract description 17
- 239000012530 fluid Substances 0.000 claims abstract description 64
- 235000015097 nutrients Nutrition 0.000 claims abstract description 39
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 38
- 238000010438 heat treatment Methods 0.000 claims abstract description 30
- 239000007788 liquid Substances 0.000 claims abstract description 29
- 238000000502 dialysis Methods 0.000 claims abstract description 21
- 239000012528 membrane Substances 0.000 claims abstract description 21
- 238000003860 storage Methods 0.000 claims abstract description 19
- 235000016709 nutrition Nutrition 0.000 claims abstract description 18
- 230000035764 nutrition Effects 0.000 claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000000243 solution Substances 0.000 claims description 38
- 238000002347 injection Methods 0.000 claims description 10
- 239000007924 injection Substances 0.000 claims description 10
- 238000007599 discharging Methods 0.000 claims description 9
- 239000000758 substrate Substances 0.000 claims description 6
- 238000010079 rubber tapping Methods 0.000 claims description 5
- 238000007667 floating Methods 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 229920001817 Agar Polymers 0.000 claims description 3
- 229920000742 Cotton Polymers 0.000 claims description 3
- 241001411320 Eriogonum inflatum Species 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 238000009434 installation Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 2
- 238000000354 decomposition reaction Methods 0.000 abstract description 4
- 238000005086 pumping Methods 0.000 abstract description 2
- 239000001963 growth medium Substances 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Sustainable Development (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Analytical Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- External Artificial Organs (AREA)
Abstract
The invention discloses a kind of microorganism heating water bath culture apparatus, including conveying peristaltic pump, blake bottle, output peristaltic pump, incubator and nutrition liquid storage tank, demarcation strip is installed inside the incubator, incubator inside points are separated with base fluid groove and water-bath heating slot by demarcation strip, blake bottle is installed inside the water-bath heating slot, tubular dialysis membrane is installed, the tubular dialysis membrane top is connected with lid, inserted with nutrient solution delivery pipe on upper lid inside the blake bottle；The convenient batch to microorganism is cultivated respectively, and temperature ensures that water temperature is between the higher limit and lower limit of heater operating temperature all the time in water-bath heating slot, is advantageous to the culture to microorganism in blake bottle；Nutrient solution pumping is convenient and swift；Base fluid intracavitary base fluid is pumped into base fluid groove by output peristaltic pump by base fluid efferent duct, the convenient base fluid discharge that base fluid intracavitary is flowed into through tubular dialysis membrane after microorganism decomposition, improves the convenience of microculture.
Description
Technical field
The present invention relates to technical field of biological culture, specifically a kind of microorganism heating water bath culture apparatus.
Background technology
In bioengineering, the culture of microorganism is common, the culture generally use culture medium training of existing microorganism
Support, i.e., using cylindricality tank body or flat box body culture, culture efficiency is low, and inconvenient for operation, when using medium culture,
Be difficult to control temperature and air conditionses required for it, easily cause microorganism because temperature is not suitable for and dead or growth and breeding delays
Slowly, microculture is caused to carry out slow, part bacterium or magazine also fall into culture medium easily by air and cause thing to give birth to
The pollution of thing culture, especially in microbial cultivation process, addition nutrient solution is difficult, using traditional manual side
Formula, the not only contaminating microorganisms when opening culture medium lid, and numerous culture mediums pay attention to operating bothersome laborious, nutrient solution addition
It is uneven, influence the effect that microorganism grows under identical conditions；And the base fluid after decomposition can not effectively be discharged, it is unfavorable for micro-
The culture of biology.
The content of the invention
It is an object of the invention to provide a kind of microorganism heating water bath culture apparatus, to solve to carry in above-mentioned background technology
The problem of going out.
To achieve the above object, the present invention provides following technical scheme：
A kind of microorganism heating water bath culture apparatus, including conveying peristaltic pump, blake bottle, output peristaltic pump, incubator and
Nutrition liquid storage tank, demarcation strip is installed inside the incubator, incubator inside points are separated with demarcation strip into base fluid groove and water-bath adds
Heat channel, blake bottle is installed inside the water-bath heating slot, tubular dialysis membrane is installed, the tubular is saturating inside the blake bottle
Analysis film top is connected with lid, inserted with nutrient solution delivery pipe, nutrient solution delivery pipe and tubular dialysis membrane internal cavities on upper lid
Connect, be set to base fluid chamber in the blake bottle outside the tubular dialysis membrane, the blake bottle bottom side is connected with base fluid efferent duct, base
Liquid efferent duct connects with base fluid chamber；Connect on the bottle stopper of the blake bottle upper end inserted with air conduit, air conduit with base fluid chamber
Logical, the air conduit passes incubator connection air cleaner, and filter cotton is provided with inside air cleaner；The nutrient solution is defeated
Pipe is sent to be located in through the demarcation strip above water-bath heating slot in conveying peristaltic pump, the nutrient solution delivery pipe other end connects liquid
Body distributor, liquid distributor are arranged on the bottom of nutrition liquid storage tank and connected with nutrient solution tank inside；The base fluid output
Pipe is located in output peristaltic pump through the demarcation strip below water-bath heating slot, the base fluid outlet of the base fluid efferent duct bottom
Connected with base fluid groove；The conveying peristaltic pump is equal with the structure for exporting peristaltic pump, the conveying peristaltic pump and output peristaltic pump
Formed by motor, wriggling roller and wriggling case, the wriggling roller is arranged in the rotating shaft of motor, and the wriggling case is set in
On the outside of wriggling roller, the wriggling roller is provided with the wriggling groove passed through for base fluid efferent duct or nutrient solution delivery pipe, and wriggling groove is edge
The discontinuous groove that wriggling roller circumferentially opens up；Installation having heaters and temperature sensor inside the water-bath heating slot；The water
Water injection pipe and overflow pipe are connected with the upside of bath heating tank, the height of the overflow pipe is more than the height of water injection pipe and is less than blake bottle
The height of upper end bottleneck, the mouth of pipe of the water injection pipe are provided with floating ball valve, and the water-bath heating slot bottom side is connected with drainpipe,
Valve is installed on drainpipe.
As the further scheme of the present invention：The vertically-mounted dividing plate of dividing plate and block that the demarcation strip is horizontally mounted by block
Composition.
As the further scheme of the present invention：The quantity of the blake bottle is 3~6 and is distributed on heating water bath trench bottom
Demarcation strip on, the blake bottle preferably heat-resisting glass material.
As the further scheme of the present invention：The tubular dialysis membrane bottom is connected with culture substrate cassette, cultivates substrate cassette
Bottom 3~5mm of laying depth agar.
As the further scheme of the present invention：The nutrient solution tank inside is provided with agitator, agitator connection motor
Rotating shaft, motor is fixed on outside nutrition liquid storage tank, and the nutrient solution tank top is provided with nutrient solution addition bucket.
As the further scheme of the present invention：The incubator bottom side is connected with discharging tube, and discharging tube connects with base fluid groove,
Tapping valve is installed on discharging tube.
As the further scheme of the present invention：The heater and temperature sensor electrically connect controller, the control
Device electrical connection motor, conveying peristaltic pump and output peristaltic pump.
Compared with prior art, the beneficial effects of the invention are as follows：A kind of microorganism heating water bath culture apparatus, structure
Rationally, easy to operate, by placing multiple blake bottles in water-bath heating slot, the convenient batch to microorganism is cultivated respectively, temperature
Degree is stable, ensures that water temperature is between the higher limit and lower limit of heater operating temperature all the time in water-bath heating slot, is advantageous to
Culture to microorganism in blake bottle；Conveying peristaltic pump is convenient to convey nutrient solution in nutrition liquid storage tank by nutrient solution delivery pipe
Into blake bottle, nutrient solution pumping is convenient and swift；Base fluid intracavitary base fluid is pumped into base fluid by output peristaltic pump by base fluid efferent duct
In groove, the convenient base fluid discharge that base fluid intracavitary is flowed into through tubular dialysis membrane after microorganism decomposition, microorganism is improved
The convenience of culture.
Brief description of the drawings
Fig. 1 is the structural representation of the present invention.
Fig. 2 is the structural representation of blake bottle in the present invention.
In figure：1- conveyings peristaltic pump, 2- blake bottles, 3- controllers, 4- heaters, 5- temperature sensors, the output of 6- base fluids
Pipe, 7- outputs peristaltic pump, 8- demarcation strips, 9- base fluids outlet, 10- incubators, 11- base fluids groove, 12- wrigglings case, 13- are wriggled
Roller, 14- discharging tubes, 15- tapping valves, 16- water-bath heating slots, 17- drainpipes, 18- floating ball valves, 19- water injection pipes, 20- overflows
Pipe, 21- air conduits, 22- air cleaners, 23- nutrient solutions delivery pipe, 24- wrigglings groove, 25- support frames, the storage of 26- nutrient solutions
Tank, 27- agitators, 28- nutrient solutions addition bucket, 29- liquid distributors, 30- motors, 31- tubulars dialysis membrane, 32- culture mediums bottom
Lid, 34- base fluid chambers on box, 33-.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained every other under the premise of creative work is not made
Embodiment, belong to the scope of protection of the invention.
Refer to Fig. 1~2, in the embodiment of the present invention, a kind of microorganism heating water bath culture apparatus, including conveying peristaltic pump
1st, blake bottle 2, output peristaltic pump 7, incubator 10 and nutrition liquid storage tank 26, demarcation strip 8 is installed inside the incubator 10, point
Dividing plate 8 is made up of 3 pieces of dividing plates being horizontally mounted and 1 piece of vertically-mounted dividing plate, and the inside points of incubator 10 are separated with base by demarcation strip 8
Liquid bath 11 and water-bath heating slot 16, blake bottle 2 is installed, the quantity of blake bottle 2 is 3~6 inside the water-bath heating slot 16
And it is distributed on the demarcation strip 8 of the bottom of water-bath heating slot 16, the preferably heat-resisting glass material of the blake bottle 2；The blake bottle 2
Inside is provided with tubular dialysis membrane 31, and the bottom of tubular dialysis membrane 31 is connected with culture substrate cassette 32, during microculture, culture medium
Bottom 3~the 5mm of laying depth of back box 32 agar, microorganism is prevented to be close to the culture bottom wall of substrate cassette 32 and influence its growth and breeding；
The top of tubular dialysis membrane 31 is connected with lid 33, inserted with nutrient solution delivery pipe 23, nutrient solution delivery pipe 23 on upper lid 33
With the connects with the internal cavities of tubular dialysis membrane 31, base fluid chamber 34, the training are set in the blake bottle 2 outside the tubular dialysis membrane 31
Support the bottom side of bottle 2 and be connected with base fluid efferent duct 6, base fluid efferent duct 6 connects with base fluid chamber 34.
Connected on the bottle stopper of the upper end of blake bottle 2 inserted with air conduit 21, air conduit 21 with base fluid chamber 34, air
Oxygen is provided to the microorganism cultivated in its internal cavities through tubular dialysis membrane 31, the air conduit 21 passes incubator 10
Air cleaner 22 is connected, the inside of air cleaner 22 is provided with the filter cotton for air filtration, improves the filtering to air
Effect, prevent from being polluted by bacterium in extraneous air or magazine during microculture.
The nutrient solution delivery pipe 23 is located in conveying peristaltic pump 1 through the demarcation strip 8 of the top of water-bath heating slot 16, institute
State the other end of nutrient solution delivery pipe 23 connection liquid distributor 29, liquid distributor 29 be arranged on nutrition liquid storage tank 26 bottom and
Connected with the inside of nutrition liquid storage tank 26, agitator 27 is installed inside the nutrition liquid storage tank 26, agitator 27 connects motor 30
Rotating shaft, motor 30 is fixed on outside nutrition liquid storage tank 26, and the top of nutrition liquid storage tank 26 is provided with adds for nutrient solution
Nutrient solution addition bucket 28；In the case where motor 30 drives agitator 27 to rotate, the nutrition of nutrient solution in nutrition liquid storage tank 26 is improved
The uniformity of material distribution, under the distribution of liquid distributor 29, ensure nutrient solution simultaneously and uniformly flow into what is connected below
In nutrient solution delivery pipe 23, conveying peristaltic pump 1 works, and conveniently realizes that nutrient solution delivery pipe 23 adds nutrient solution around each culture
In bottle 2, breed and use for the micro-organisms in tubular dialysis membrane 31, the base fluid after microorganism decomposition is dialysed through tubular
Film 31 is flowed into base fluid chamber 34, convenient to be discharged from base fluid efferent duct 6.
The base fluid efferent duct 6 is located in output peristaltic pump 7 through the demarcation strip 8 of the lower section of water-bath heating slot 16, described
The base fluid outlet 9 of the bottom of base fluid efferent duct 6 connects with base fluid groove 11, and the bottom side of incubator 10 is connected with discharging tube 14, row
Liquid pipe 14 is connected with base fluid groove 11, and tapping valve 15 is provided with discharging tube 14, and output peristaltic pump 7 works, and passes through base fluid efferent duct 6
Base fluid in base fluid chamber 34 is pumped into base fluid groove 11, facilitates the discharge of base fluid in base fluid chamber 34, tapping valve 15 is opened, passes through discharge opeing
Pipe 14 discharges the base fluid of base fluid groove 11.
The conveying peristaltic pump 1 is equal with the structure for exporting peristaltic pump 7, and the conveying peristaltic pump 1 and output peristaltic pump 7 are equal
It is made up of motor, wriggling roller 13 and wriggling case 12, the wriggling roller 13 is arranged in the rotating shaft of motor, the wriggling case 12
The outside of wriggling roller 13 is set in, the wriggling roller 13 is provided with the wriggling passed through for base fluid efferent duct 6 or nutrient solution delivery pipe 23
Groove 24, wriggling groove 24 are the discontinuous groove circumferentially opened up along wriggling roller 13, and when wriggling roller 13 rotates, wriggling groove 24 is discontinuously located
It is convenient to extrude liquid push in base fluid efferent duct 6 or nutrient solution delivery pipe 23.
The inside of water-bath heating slot 16 installation having heaters 4 and temperature sensor 5, the heater 4 and TEMP
Device 5 electrically connects controller 3, and the controller 3 electrically connects motor 30, conveying peristaltic pump 1 and output peristaltic pump 7；The water-bath
The upside of heating tank 16 is connected with water injection pipe 19 and overflow pipe 20, and the height of the overflow pipe 20 is more than the height of water injection pipe 19 and low
In the height of the upper end bottleneck of blake bottle 2, the mouth of pipe of the water injection pipe 19 is provided with floating ball valve 18, the bottom of water-bath heating slot 16
Side is connected with drainpipe 17, and valve is provided with drainpipe 17；When microculture carries out Heating Water, set by controller 3
The higher limit and lower limit of the operating temperature of heater 4 are put, then starts heater 4 and works, temperature sensor 5 is to water-bath heating slot
Water temperature detects in 16, when temperature is higher than 4 higher limit of heater, the break-off of heater 4, when temperature is less than the lower limit of heater 4
During value, heater 4 opens up work, ensure in water-bath heating slot 16 water temperature all the time the higher limit in the operating temperature of heater 4 and
Between lower limit, be advantageous to the culture to microorganism in blake bottle 2.
The invention is not limited in above-described embodiment, on the basis of technical scheme disclosed by the invention, the skill of this area
Art personnel are according to disclosed technology contents, it is not necessary to which performing creative labour can makes one to some of which technical characteristic
A little simple modification, equivalent change and modifications, are belonged in the range of technical solution of the present invention.
, it is necessary to illustrate in the description of this specification, unless otherwise clearly defined and limited, term " setting ",
" connected " and " connection " should be interpreted broadly, for example, it may be being fixedly connected or being detachably connected, or integratedly be connected
Connect；Can be joined directly together, can also be indirectly connected by intermediary, can be the connection of two element internals.For this
For the those of ordinary skill in field, the concrete meaning of above-mentioned term in the present invention can be understood with concrete condition.
Claims (7)
1. a kind of microorganism heating water bath culture apparatus, including conveying peristaltic pump (1), blake bottle (2), output peristaltic pump (7), training
Support case (10) and nutrition liquid storage tank (26), it is characterised in that demarcation strip (8), demarcation strip are installed inside the incubator (10)
(8) incubator (10) inside points are separated with base fluid groove (11) and water-bath heating slot (16), pacified inside the water-bath heating slot (16)
Equipped with blake bottle (2), tubular dialysis membrane (31) is installed inside the blake bottle (2), tubular dialysis membrane (31) top connects
Lid (33) is connected to, it is upper to cover on (33) inserted with nutrient solution delivery pipe (23), nutrient solution delivery pipe (23) and tubular dialysis membrane
(31) connects with the internal cavities, the outside blake bottle (2) of the tubular dialysis membrane (31) is interior to be set to base fluid chamber (34), the blake bottle
(2) bottom side is connected with base fluid efferent duct (6), and base fluid efferent duct (6) connects with base fluid chamber (34)；Blake bottle (2) upper end
Connected on bottle stopper inserted with air conduit (21), air conduit (21) with base fluid chamber (34), the air conduit (21) passes training
Case (10) connection air cleaner (22) is supported, filter cotton is provided with inside air cleaner (22)；The nutrient solution delivery pipe (23)
Demarcation strip (8) above through water-bath heating slot (16) is located in conveying peristaltic pump (1), and the nutrient solution delivery pipe (23) is another
One end connection liquid distributor (29), liquid distributor (29) be arranged on nutrition liquid storage tank (26) bottom and with nutrition liquid storage tank
(26) it is internal to connect；The base fluid efferent duct (6) is located in output through the demarcation strip (8) below water-bath heating slot (16) and wriggled
In pump (7), the base fluid outlet (9) of base fluid efferent duct (6) bottom connects with base fluid groove (11)；The conveying peristaltic pump
(1) and output peristaltic pump (7) structure it is equal, it is described conveying peristaltic pump (1) and export peristaltic pump (7) by motor, wriggling roller
(13) it is arranged on wriggling case (12) composition, the wriggling roller (13) in the rotating shaft of motor, the wriggling case (12) is arranged
On the outside of wriggling roller (13), the wriggling roller (13) is provided with what is passed through for base fluid efferent duct (6) or nutrient solution delivery pipe (23)
Wriggling groove (24), wriggling groove (24) are the discontinuous groove circumferentially opened up along wriggling roller (13)；In the water-bath heating slot (16)
Portion's installation having heaters (4) and temperature sensor (5)；Water injection pipe (19) and overflow are connected with the upside of the water-bath heating slot (16)
To manage (20), the height of the overflow pipe (20) is more than the height of water injection pipe (19) and is less than the height of blake bottle (2) upper end bottleneck,
The mouth of pipe of the water injection pipe (19) is provided with floating ball valve (18), and water-bath heating slot (16) bottom side is connected with drainpipe
(17) valve, is installed on drainpipe (17).
A kind of 2. microorganism heating water bath culture apparatus according to claim 1, it is characterised in that the demarcation strip (8)
The dividing plate and the vertically-mounted dividing plate of (1) block being horizontally mounted by (3) block form.
A kind of 3. microorganism heating water bath culture apparatus according to claim 1, it is characterised in that the blake bottle (2)
Quantity 3~6 and to be distributed on the demarcation strip (8) of water-bath heating slot (16) bottom, the blake bottle (2) is preferably heat-resisting
Glass material.
A kind of 4. microorganism heating water bath culture apparatus according to claim 1, it is characterised in that the tubular dialysis membrane
(31) bottom is connected with culture substrate cassette (32), cultivates substrate cassette (32) bottom 3~5mm of laying depth agar.
A kind of 5. microorganism heating water bath culture apparatus according to claim 1, it is characterised in that the nutrition liquid storage tank
(26) internal to be provided with agitator (27), agitator (27) connects the rotating shaft of motor (30), and motor (30) is fixed on nutrient solution storage
Tank (26) is outside, and nutrient solution addition bucket (28) is provided with the top of the nutrition liquid storage tank (26).
A kind of 6. microorganism heating water bath culture apparatus according to claim 1, it is characterised in that the incubator (10)
Bottom side is connected with discharging tube (14), and discharging tube (14) is connected with base fluid groove (11), and tapping valve (15) is provided with discharging tube (14).
A kind of 7. microorganism heating water bath culture apparatus according to claim 5, it is characterised in that the heater (4)
Electrically connect controller (3) with temperature sensor (5), controller (3) the electrical connection motor (30), conveying peristaltic pump (1) and
Export peristaltic pump (7).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710556051.6A CN107475093A (en) | 2017-07-10 | 2017-07-10 | A kind of microorganism heating water bath culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710556051.6A CN107475093A (en) | 2017-07-10 | 2017-07-10 | A kind of microorganism heating water bath culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107475093A true CN107475093A (en) | 2017-12-15 |
Family
ID=60596050
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710556051.6A Pending CN107475093A (en) | 2017-07-10 | 2017-07-10 | A kind of microorganism heating water bath culture apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107475093A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110029057A (en) * | 2019-04-19 | 2019-07-19 | 烟台职业学院 | A kind of biotechnology cell culture apparatus |
| CN115786121A (en) * | 2023-02-07 | 2023-03-14 | 潍坊医学院附属医院 | Cell culture device |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101434902A (en) * | 2008-12-18 | 2009-05-20 | 崔生发 | Open type cyclic tubular microbial dialysis cultivation system |
| CN204162717U (en) * | 2014-10-17 | 2015-02-18 | 漯河医学高等专科学校 | A kind of microorganism continuous culture device |
| CN205133621U (en) * | 2015-11-13 | 2016-04-06 | 山东省鲁南工程技术研究院 | Microorganism culture apparatus |
| CN205590705U (en) * | 2016-04-26 | 2016-09-21 | 郑州职业技术学院 | Microorganism water bath with thermostatic control fermenting installation |
| CN205954026U (en) * | 2016-05-09 | 2017-02-15 | 北京银丰鼎诚生物工程技术有限公司 | Split type perfusion culture system of nerve cord cell |
| CN206308342U (en) * | 2016-12-01 | 2017-07-07 | 塔里木大学 | A kind of multifunctional microbial incubator |
-
2017
- 2017-07-10 CN CN201710556051.6A patent/CN107475093A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101434902A (en) * | 2008-12-18 | 2009-05-20 | 崔生发 | Open type cyclic tubular microbial dialysis cultivation system |
| CN204162717U (en) * | 2014-10-17 | 2015-02-18 | 漯河医学高等专科学校 | A kind of microorganism continuous culture device |
| CN205133621U (en) * | 2015-11-13 | 2016-04-06 | 山东省鲁南工程技术研究院 | Microorganism culture apparatus |
| CN205590705U (en) * | 2016-04-26 | 2016-09-21 | 郑州职业技术学院 | Microorganism water bath with thermostatic control fermenting installation |
| CN205954026U (en) * | 2016-05-09 | 2017-02-15 | 北京银丰鼎诚生物工程技术有限公司 | Split type perfusion culture system of nerve cord cell |
| CN206308342U (en) * | 2016-12-01 | 2017-07-07 | 塔里木大学 | A kind of multifunctional microbial incubator |
Non-Patent Citations (1)
| Title |
|---|
| 刁治民主编: "《微生物学 修订版》", 科学技术文献出版社, pages: 77 - 93 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110029057A (en) * | 2019-04-19 | 2019-07-19 | 烟台职业学院 | A kind of biotechnology cell culture apparatus |
| CN115786121A (en) * | 2023-02-07 | 2023-03-14 | 潍坊医学院附属医院 | Cell culture device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102382766B (en) | Tidal bioreactor for animal cell culture | |
| CN106467892B (en) | A kind of automatic cytological amplification system | |
| CN204058466U (en) | Solid-state fermenter | |
| CN110656043A (en) | Microbial fermentation tank for producing liquid feed additive | |
| CN107475093A (en) | A kind of microorganism heating water bath culture apparatus | |
| KR101657489B1 (en) | Circulation-type high-density culturing apparatus of microalgae using air | |
| CN207313594U (en) | A kind of microorganism heating water bath culture apparatus | |
| CN110656044A (en) | Novel intelligent microbial fermentation device | |
| KR101770418B1 (en) | Living Thing Cultivation Machine with Special Valve | |
| CN206502818U (en) | A kind of microbial cultivation device | |
| CN103865753B (en) | Liquid and solid combined fungicide fermentation and preparation system | |
| CN206843447U (en) | A kind of gravity flow photosynthetic reaction device | |
| CN203537926U (en) | Edible fungus liquid culture production and cultivation system | |
| CN203807476U (en) | Fermentation tank | |
| CN211688986U (en) | Preparation facilities of bacillus subtilis zymotic fluid suspension | |
| CN215250779U (en) | Microbial cultivation device for microbial functionality experiments | |
| CN212955152U (en) | Heating type microorganism heat preservation culture tank | |
| CN201660631U (en) | Bacterial culture tank for pharmacy | |
| CN207079239U (en) | Solid state fermentation system | |
| CN102168010A (en) | Photobioreactor for culturing microorganisms | |
| CN209722163U (en) | Low-temperature heating system for cultivation | |
| CN206375727U (en) | The sewage disposal system of the outer sludge acclimatization culture of system | |
| CN110423674B (en) | Microorganism precipitation and propagation conveying device | |
| CN213569706U (en) | Biochemical device with detachable inner water outlet pipe for sewage treatment | |
| CN213680677U (en) | Fermentation device of special strain for industrial waste gas purification |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171215 |