CN101665764A - Dual-phase blood culture system - Google Patents
Dual-phase blood culture system Download PDFInfo
- Publication number
- CN101665764A CN101665764A CN 200910104787 CN200910104787A CN101665764A CN 101665764 A CN101665764 A CN 101665764A CN 200910104787 CN200910104787 CN 200910104787 CN 200910104787 A CN200910104787 A CN 200910104787A CN 101665764 A CN101665764 A CN 101665764A
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- China
- Prior art keywords
- solid phase
- substratum
- culture medium
- blood
- liquid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
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- Engineering & Computer Science (AREA)
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- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
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- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to a dual-phase blood culture system comprising a liquid phase culture medium part, an indicator and solid phase culture medium part and a drainage member. The liquid phase culture medium part is a blood culture bottle filled with a liquid phase culture medium, and a catheter is connected in the plug of the blood culture bottle and is inserted into at least the position of the liquid level of the liquid phase culture medium in the bottle. The indicator and solid phase culture medium part is a container filled with solid phase culture medium slides. A liquid inlet channelis arranged on the container cover and the inner end of the liquid inlet channel is connected with the solid phase culture medium slides. Solid phase culture media are loaded at two sides or one sideof each solid phase culture medium slide. The drainage member comprises a hose and a needle head. The needle head is connected at one end of the hose and is inserted into the plug of the blood culturebottle to enter the catheter. The other end of the hose is connected with the outer end of the liquid inlet channel of the container of the indicator and solid phase culture medium part. The liquid phase culture medium part and the indicator and solid phase culture medium part are connected in parallel by the drainage member and are installed in a bracket. The system has the advantages as follows: the system has simple structure, complete functions, high indication sensitivity, and simple and convenient operation, can automatically transmit, and has both liquid phase and solid phase simultaneously, etc.
Description
Technical field
The present invention relates to a kind of system that is used for blood cultivation.
Background technology
At present, using more general blood culture system clinically is the Singal blood culture system of Britain OXOID company.It is a tubule blood cultivation bottle with indication mechanism, the blood cultivation bottle is connected up and down by injection needles with indication mechanism, needle tip inserts certain position under bottle liquid level of interior nutrient chemical base, when producing gas in the bacterial growth in the blood cultivation bottle air pressure in the bottle is raise gradually, liquid in the bottle is depressed in the tubule as indication mechanism by injection needles, makes the positive prompting that obtains that naked eyes observe at an easy rate.Its advantage is simple in structure, and is simple to operate, safeguards easily, if there is not positive prompting, then need not to remake further transferred species and handles, and it is few therefore to receive the external interference factor.Its shortcoming is: because indication mechanism is last, action of gravity shows that its susceptibility weakens; Do not have the transferred species system,, not only increase workload but also expend time in if positive prompting still needs the professional and technical personnel to do further transferred species and separates.
Summary of the invention
The objective of the invention is to defective at the prior art existence, a kind of dual-phase blood culture system of automatic transferred species is provided, it has under the prerequisite of simple structure in reservation, improve the susceptibility of indicating section on the one hand, increase the transferred species separate part on the other hand, make its more perfect functions, and improve the pollution capacity of avoiding of system.
Technical scheme of the present invention is as follows:
A kind of whole blood culture systems is made up of liquid phase culture medium part, indication and solid phase substratum part and drain spare.
Described liquid phase culture medium part is a blood cultivation bottle that liquid phase medium is housed, and is connected with a conduit in the bottle stopper of blood cultivation bottle, and conduit extends under the interior liquid phase medium liquid level of insertion bottle;
Partly for the container of solid phase substratum slide glass is housed, its container cover is provided with feed pathway for described indication and solid phase substratum, the inner solid phase substratum slide glass that connects of feed pathway, and two-sided or single face load has the solid phase substratum on the solid phase substratum slide glass;
Described drain spare comprises a flexible pipe and a syringe needle, syringe needle is connected flexible pipe one end, and the bottle stopper of insertion blood cultivation bottle, enter in the conduit, the other end of flexible pipe is connected with the container feed pathway outer end of indication and solid phase substratum part, liquid phase culture medium part and indication and solid phase substratum part are connected in parallel by drain spare, are contained in the mounting.
Use the system of said structure, when having bacterial growth to produce gas in the liquid phase medium, intrasystem air pressure raises, liquid is pressed in indication in parallel with it and the solid phase substratum part, is convenient to observe, and convenient automatic transferred species is separated, save time, simplify the operation, can one realize that cultivation, indication separate perfect in shape and function with transferred species.Simultaneously, adopt the form of two portions parallel connection, can weaken the influence of action of gravity, improve display sensitivity its susceptibility.
For liquid import indication and solid phase substratum part unblocked, the coupling end of the feed pathway on solid phase substratum slide glass and the container cover is designed to insert or sleeve type, and is provided with the diversion trench of downward connection at coupling end outer wall or inwall.In addition, the solid phase substratum stably is set on the solid phase substratum slide glass for convenience, difficult drop-off, the main body section of solid phase substratum slide glass is designed to the U-lag shape, and further be provided with the localized raised line of solid phase substratum in the U-lag baseplane, U-lag both sides and lower end are provided with inside draw-in groove or latch.Importing liquid for convenience evenly flows on the solid phase substratum, be provided with the flow-guiding structure that is communicated with coupling end and main body with the transition portion of coupling end above solid phase substratum slide glass main body, this flow-guiding structure is diversion trench or the irregular curb that forms between the coarse particles of uneven distribution.
In addition, not contaminated for fear of the syringe needle of drain spare, its syringe needle is fixed in the sheath, when not in use, avoid syringe needle and extraneous contacting, sheath is placed in blood cultivation bottle bottleneck.
Also have, high-temperature high-voltage sterilizing sterilizing for convenience of culturing bottle, outer wall in the neck Lower Half of the bottle stopper of blood cultivation bottle is provided with air channel longitudinally, and designed a circle latch at the outer wall of neck Lower Half, it is in when sterilization partly clogs the bottleneck state, prevent to come off, be convenient to sterilization in the bottle again.
Therefore, by above-mentioned improvement, native system has simple in structure, transferred species automatically, and the liquid phase solid phase possesses simultaneously, perfect in shape and function, display sensitivity height, the tangible advantage of grade easy and simple to handle.
Description of drawings
Fig. 1 is the structural representation of native system;
Fig. 2 is the vertical view of Fig. 1;
Fig. 3 A is the structural representation of solid phase substratum slide glass;
Fig. 3 B is the B-B sectional view of Fig. 3 A;
Fig. 3 C is the A-A sectional view of Fig. 3 A;
Fig. 4 A is the structural representation of solid phase substratum slide glass;
Fig. 4 B is the B-B sectional view of Fig. 4 A;
Fig. 4 C is the A-A sectional view of Fig. 4 A;
Fig. 5 A is the structural representation of the bottle stopper of culturing bottle;
Fig. 5 B is the A-A sectional view of Fig. 5 A.
Embodiment
Referring to Fig. 1 and Fig. 2, this blood culture system is made up of three parts:
Liquid phase culture medium part 1 also is the blood cultivation bottle, in the bottle liquid phase medium is housed;
Indication and solid phase substratum part 3: positive when cultivating, also be that liquid phase culture medium part produces gas when inoculation is gone into blood preparation and turned out bacterium, its pressure is pressed into the mixing liquid of liquid nutrient medium and sample in indication and the solid phase substratum part, indication is cultivated positive, this mixing liquid (bacterium liquid) is flowed through and is fixed in nutrient agar surface on the slide glass simultaneously, thereby nutrient agar is reached the purpose of automatic transferred species bacterium by bacterium on the automatic vaccination.
Drain spare 2, with above two portions parallel connection, and transporting liquid.
Below the concrete structure of each several part is elaborated:
Liquid phase culture medium part 1: described liquid phase culture medium part is a blood cultivation bottle 11 that liquid phase medium 12 is housed, and is connected with a conduit 13 in the bottle stopper 14 of blood cultivation bottle, and conduit 13 extends in the insertion bottle at least to the liquid phase medium liquid level position.In use, conduit can be delivered to indication and solid phase substratum part by drain spare with bacterium liquid.
Indication and solid phase substratum part 3: for the transparent vessel 32 of solid phase substratum slide glass is housed, be used to hold the liquid (bacterium liquid) that is imported on the one hand, be used to hold solid phase substratum and slide glass 32 thereof on the one hand, because transparent and be convenient to observe.Its container cover 33 is provided with feed pathway 34, and the feed pathway 34 inner solid phase substratum slide glasses 32 that connect are beneficial to the liquid of drain spare 2 guiding is imported in the container.Two-sided or single face load has the solid phase substratum on the solid phase substratum slide glass 32.
Drain spare 2: comprise a flexible pipe 21, a syringe needle 22 and a sleeve 23, syringe needle 22 is connected flexible pipe 21 1 ends, and be fixed in the sleeve 23, when cover upper bush 23 on the bottleneck of culturing bottle, the needle point of syringe needle 22 can puncture bottle stopper 14, and inserts in the bottle inner catheter, makes bottle interior and the extraneous purpose that communicates to reach, and sleeve 23 can protect syringe needle not to be subjected to outside contamination simultaneously, and way one is can be with needle guard on the pinhead sleeve; The 2nd, available film or lid, stopper are closed in syringe needle in the sleeve in the sleeve open end.The other end of flexible pipe 21 is connected with container feed pathway 34 outer ends of indication and solid phase substratum part.
Sample is hatched when cultivating in inoculation, and liquid phase culture medium part 1 and indication and solid phase substratum part 3 are connected in parallel by drain spare 2, are contained in the mounting 4.
The structure of solid phase substratum slide glass 32 has following several form:
Referring to Fig. 3 A, Fig. 3 B and Fig. 3 C, the coupling end 321 that solid phase substratum slide glass 32 is connected with feed pathway 34 on the container cover 33 adopts insert or sleeve types, and be provided with the diversion trench 322 of downward connection at coupling end 321 outer walls or inwall, but main body 324 cross sections of solid phase substratum slide glass are the U-lag shape of load solid phase substratum.
Referring to Fig. 4 A, Fig. 4 B and Fig. 4 C, the top of solid phase substratum slide glass main body 324 also can be provided with the flow-guiding structure that is communicated with coupling end and main body with the transition portion 323 of coupling end 321, this flow-guiding structure can be the irregular curb that forms between the coarse particles 327 of uneven distribution, it also can be the diversion trench 329 among Fig. 4 A, be beneficial to make next liquid (bacterium liquid) dispersion train of guiding through the solid phase substratum, or directly form some little teeth groove in solid phase substratum upper end.
On the U-lag baseplane of solid phase substratum slide glass main body 324, be provided with the localized raised line 325 of solid phase substratum, be formed with inside draw-in groove 326 in both sides and lower end, or latch 328 as showing among Fig. 4 A, Fig. 4 B, Fig. 4 C, be convenient to when pouring into the solid phase substratum, substratum flows into wherein, after treating the cooling of solid phase substratum, substratum is firmly secured on the slide glass.Prevent that the liquid (bacterium liquid) that the solid phase substratum is directed into from washing away or soaking and coming off.
The structure of the bottle stopper 14 of blood cultivation bottle is referring to Fig. 5 A and Fig. 5 B, outer wall in its neck Lower Half is distributed with 4 air channels 141 longitudinally, and be that stopper has designed a circle latch 142 when partly filling in state, when producing sterilization, can stably be in half plug state to keep it, be beneficial to the balance of bottle external and internal pressure and reach the purpose of sterilization
Claims (6)
1, dual-phase blood culture system is made up of liquid phase culture medium part, indication and solid phase substratum part and drain spare, it is characterized in that:
Described liquid phase culture medium part is a blood cultivation bottle that liquid phase medium is housed, and is connected with a conduit in the bottle stopper of blood cultivation bottle, and conduit extends in the insertion bottle at least to the liquid phase medium liquid level position;
Partly for the container of solid phase substratum slide glass is housed, its container cover is provided with feed pathway for described indication and solid phase substratum, the inner solid phase substratum slide glass that connects of feed pathway, and two-sided or single face load has the solid phase substratum on the solid phase substratum slide glass;
Described drain spare comprises a flexible pipe and a syringe needle, syringe needle is connected flexible pipe one end, and the bottle stopper of insertion blood cultivation bottle, enter in the conduit, the other end of flexible pipe is connected with the container feed pathway outer end of indication and solid phase substratum part, liquid phase culture medium part and indication and solid phase substratum part are connected in parallel by drain spare, are contained in the mounting.
2, dual-phase blood culture system according to claim 1, it is characterized in that: the coupling end of the feed pathway on described solid phase substratum slide glass and the container cover adopts insert or sleeve type, and is provided with the diversion trench of downward connection at coupling end outer wall or inwall; But the main body section of solid phase substratum slide glass is the U-lag shape of appendix solid phase substratum, and the top of solid phase substratum slide glass main body is provided with the flow-guiding structure that is communicated with coupling end and main body with the transition portion of coupling end.
3, dual-phase blood culture system according to claim 2 is characterized in that: the flow-guiding structure of described transition portion is diversion trench or the irregular curb that forms between the coarse particles of uneven distribution.
4, dual-phase blood culture system according to claim 2 is characterized in that: the U-lag baseplane of described solid phase substratum slide glass main part is provided with the localized raised line of solid phase substratum, and there are inside draw-in groove or latch in U-lag both sides and lower end.
5, dual-phase blood culture system according to claim 1 and 2 is characterized in that: the syringe needle of described drain spare is fixed in the sheath, and sheath is placed in blood cultivation bottle bottleneck.
6, dual-phase blood culture system according to claim 1 and 2 is characterized in that: the outer wall of the neck Lower Half of the bottle stopper of described blood cultivation bottle is distributed with air channel longitudinally, and has designed a circle latch at the outer wall of neck Lower Half.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910104787 CN101665764A (en) | 2009-09-04 | 2009-09-04 | Dual-phase blood culture system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910104787 CN101665764A (en) | 2009-09-04 | 2009-09-04 | Dual-phase blood culture system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101665764A true CN101665764A (en) | 2010-03-10 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200910104787 Pending CN101665764A (en) | 2009-09-04 | 2009-09-04 | Dual-phase blood culture system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR3075816A1 (en) * | 2017-12-26 | 2019-06-28 | Centre National De La Recherche Scientifique (Cnrs) | DEVICE FOR CULTURING MICROORGANISMS |
-
2009
- 2009-09-04 CN CN 200910104787 patent/CN101665764A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR3075816A1 (en) * | 2017-12-26 | 2019-06-28 | Centre National De La Recherche Scientifique (Cnrs) | DEVICE FOR CULTURING MICROORGANISMS |
| WO2019129787A1 (en) * | 2017-12-26 | 2019-07-04 | Centre National De La Recherche Scientifique (Cnrs) | Device for cultivating microorganisms |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20100310 |