CN114214169B

CN114214169B - PCR plate for preventing cross infection

Info

Publication number: CN114214169B
Application number: CN202111428927.1A
Authority: CN
Inventors: 刘子贤; 刘刚; 刘子德; 袁义勇; 赵华; 唐波
Original assignee: Meidong Huicheng Life Technology Kunshan Co ltd
Current assignee: Meidong Huicheng Life Technology Kunshan Co ltd
Priority date: 2021-11-28
Filing date: 2021-11-28
Publication date: 2023-10-13
Anticipated expiration: 2041-11-28
Also published as: CN114214169A; WO2023093877A1

Abstract

The utility model relates to the technical field of PCR plates, in particular to a cross infection prevention PCR plate, which comprises a reaction plate, wherein the reaction plate is clamped on the upper surface of a lower supporting plate in a matched manner, a plurality of reaction cylinders are arranged on the surface of the reaction plate, the upper surface of each reaction cylinder is sealed by a sealing plate, reaction cylinder clamping grooves with the same number as that of the reaction cylinders are formed in the surface of the reaction plate, drainage grooves are formed between the opposite reaction cylinder clamping grooves, the drainage grooves are formed in the upper surface of the reaction plate, skirt edges are formed in the side walls of the reaction plate, a drainage groove upper cover plate with the same number as that of the reaction cylinders is fixedly connected to the lower surface of the upper sealing plate in a matched manner, and the drainage groove upper cover plate is clamped on the reaction cylinders in a matched manner.

Description

PCR plate for preventing cross infection

Technical Field

The utility model relates to the technical field of PCR plates, in particular to a cross-infection prevention PCR plate.

Background

The PCR plate is a carrier which is mainly used as a primer, a buffer solution and the like for participating in amplification reaction in a polymerase chain reaction, is widely used in a PCR laboratory, and is generally covered with a sealing plate above a reaction tube in order to prevent cross infection caused by nutrient solution in adjacent reaction tubes when a PCR cultured cell is subjected to scientific research, so that the hidden danger of cross infection is reduced, and is widely used in the PCR laboratory, such as the application number of CN201720555830.X, and the PCR plate with the PCR cover comprises a PCR plate body and two PCR covers, wherein the two PCR covers are movably fixed on the PCR plate body; the PCR plate body is provided with reaction tubes, hollow tubes are fixed on two sides of the PCR plate body, and the two hollow tubes are smaller than half of the length of the PCR plate body and are positioned at two ends of the PCR plate body; the two ends of the PCR plate body are provided with fixing rings; the PCR cover is transparent as a whole, a plurality of loops are arranged on the PCR cover, and the bottom surface of each loop is positioned on the same horizontal plane; one side of the PCR cover is provided with a rotating shaft, and the other side of the PCR cover is provided with a fixing buckle. According to the utility model, each reaction tube can be sealed, each reaction tube can be separated, the experimental accuracy of the PCR reaction is improved, convenience is brought to the operation of the PCR reaction, the experiment in the reaction tube can be observed in real time while the reaction tube is sealed, the PCR plate body and the PCR cover hole can be detached, and the PCR cover can seal each reaction tube well.

However, the above solution has the following drawbacks in use: the above-mentioned application equipment only adopts simple ring cover to seal, but seal structure is simple, at the in-process of the inside cell of culture of standing, produces easily not hard up, and then causes cross infection's hidden danger through the air flow, when dripping into the nutrient solution in the reaction section of thick bamboo inside simultaneously, because of the inside volume of reaction section of thick bamboo and bore are less, makes the nutrient solution drop fall on the board easily and can cause cross infection equally when wiping.

Disclosure of Invention

(one) solving the technical problems

Aiming at the defects that in the prior art, only a simple ring sleeve is adopted for sealing, but the sealing structure is simple, looseness is easy to generate in the process of static culture of internal cells, and cross infection is caused by air flow, the utility model provides the PCR plate for preventing cross infection.

(II) technical scheme

In order to achieve the above purpose, the technical scheme adopted by the utility model is as follows:

the utility model provides a prevent cross infection's PCR board, includes the reaction plate, reaction plate cooperation chucking is at the upper surface of bottom plate, the surface of reaction plate is provided with a plurality of reaction cylinder, every the upper surface of reaction cylinder seals through the seal die plate, the reaction cylinder draw-in groove that equals with reaction cylinder quantity is seted up on the surface of reaction plate, every row relatively be provided with the drainage groove between the reaction cylinder draw-in groove, just the upper surface at the reaction plate is seted up to the drainage groove, the avris of reaction plate is provided with the shirt rim, the lower surface of bottom plate is provided with four lower dead levers with transverse central line symmetry, and every down the dead lever carries out the slip of short distance through the trimming module, the lower surface of going up the seal die plate still fixedly connected with and the equal drainage groove upper cover plate of reaction cylinder quantity, drainage groove upper cover plate cooperation chucking is on the reaction.

Preferably, the reaction plate is rectangular, the skirt is fixedly connected to the long sides and the wide sides of the reaction plate, and the drainage groove extends to the wide side of the reaction plate.

Preferably, round holes are formed in the outer wall of each reaction needle cylinder clamping groove, and each round hole is formed in the upper surface of the reaction plate.

Preferably, an inclined plane is arranged on the inner wall of each round hole, and each round hole is matched and clamped with an upper cover plate of the reaction needle cylinder.

Preferably, the upper cover plate of the reaction cylinder is fixedly connected to the lower surface of the film sealing plate, and the outer wall of the upper cover plate of the reaction cylinder is fixedly connected with an edge sealing air bag.

Preferably, the lower surface of the film sealing plate is fixedly connected with drainage groove upper cover plates, and the end part of each drainage groove upper cover plate is fixedly connected with a sealing plate.

Preferably, the fine adjustment assembly comprises a worm wheel, a lower fixing rod, a worm and a knob, wherein the knob is fixedly connected to one end of the worm, the worm is rotationally connected to the inside of the lower supporting plate, the end part connected with the knob is located outside the lower supporting plate, the worm is connected with the worm wheel in a meshed mode, and the worm wheel is arranged inside the lower supporting plate.

Preferably, a screw hole is formed in the worm wheel and is in threaded fit with the screw rod, the upper end and the lower end of the screw rod are fixedly connected with lower fixing rods, the upper end of the screw rod is located on the inner wall of the lower supporting plate, the lower end of the screw rod is located on the inner wall of the lower supporting plate, and the lower end of the lower fixing rod is fixedly connected with a ball.

Preferably, a horizontal groove is formed in one side of the lower supporting plate, rolling balls are arranged in the horizontal groove, an upper plate is fixedly connected to the upper surface of the lower supporting plate, each upper plate is provided with a storage inner hole, a limit hole is formed in the upper surface of the lower supporting plate, and a limit rod is clamped in the limit hole in a matched mode.

Preferably, a U-shaped groove is formed in the inner wall of the skirt, a round rod is fixedly connected to the middle of the U-shaped groove, a first clamping groove is formed in the upper end of the round rod, and a second clamping groove is formed in the lower end of the round rod.

Compared with the prior art, the utility model has the following beneficial effects:

1. firstly, each reaction needle cylinder is placed on a reaction needle cylinder clamping groove, then nutrient solution is poured into the reaction needle cylinder, a reaction plate is placed on a lower supporting plate for limiting and fixing, when the nutrient solution is dripped, if the nutrient solution is dripped, the nutrient solution drops onto the plate because of smaller volume and caliber in the reaction cylinder, cross infection can be caused when the nutrient solution drops onto the plate, the knob can be rotated at the moment, the knob can be rotated through the matching rotation of a turbine and a worm, and meanwhile, a screw rod sleeve and a screw rod are matched, so that a lower fixing rod moves downwards, a ball arranged at the lower end of the reaction plate can stably support the reaction plate when the reaction plate deflects to one side, and the nutrient solution dripped on the reaction plate flows out through a drainage groove, so that the problem of cross infection caused by wiping is avoided.

2. Meanwhile, the edge sealing air bags arranged at the upper ends are extruded through the edge sealing air bags, so that the problem of cross infection inside a reaction needle cylinder caused by air circulation is avoided, the side edges of the film sealing plates are firstly slidably mounted along the first clamping grooves, when the film sealing plates are clamped to the skirt rims, the film sealing plates are pressed downwards forcefully, the PVC material is adopted to have the elastic characteristic, the two sides of the film sealing plates are enabled to move downwards through smooth rods, the two sides of the film sealing plates are aligned to limit through the smooth rods, the traditional glue layer fixing is replaced, the problem of cross infection caused by later loosening is avoided, and stable conditions are provided for culturing PCR plate cells.

Drawings

FIG. 1 is a schematic diagram of a cross-infection preventing PCR plate according to the present utility model;

FIG. 2 is a schematic diagram of the structure of a film sealing plate, a reaction plate and a lower supporting plate of a PCR plate for preventing cross infection;

FIG. 3 is a schematic bottom view of a membrane sealing plate, a reaction plate and a bottom support plate of a cross-infection prevention PCR plate;

FIG. 4 is an enlarged schematic view of the structure of the cross-infection preventing PCR plate of FIG. 3B according to the present utility model;

FIG. 5 is an enlarged schematic view of the structure of the cross-infection preventing PCR plate shown in FIG. 2A;

FIG. 6 is a schematic diagram showing the structure of a reaction plate of a cross-infection preventing PCR plate according to the present utility model;

FIG. 7 is a schematic diagram of a cross-infection preventing PCR plate trimming assembly according to the present utility model;

FIG. 8 is an enlarged schematic view of the structure of the cross-infection preventing PCR plate of FIG. 6.

In the figure: 1. film sealing plates; 101. an upper cover plate of the drainage groove; 102. a sealing plate; 103. an edge banding air bag; 104. an upper cover plate of the reaction cylinder; 2. a lower support plate; 3. a rolling ball; 4. a horizontal slot; 5. a fine tuning assembly; 501. a screw rod; 502. a worm wheel; 503. a lower fixing rod; 504. a ball; 505. a worm; 506. a knob; 6. a reaction plate; 601. a first clamping groove; 602. a U-shaped groove; 603. a round slide bar; 604. a second clamping groove; 605. a reaction cylinder clamping groove; 606. drainage grooves; 607. an inclined plane; 608. a round hole; 7. storing the inner hole; 8. a limiting hole; 9. an upper plate; 10. a limit rod; 11. a reaction cylinder; 12. and a skirt edge.

Detailed Description

The technical solutions of the embodiments of the present utility model will be clearly and completely described below in conjunction with the embodiments of the present utility model, and it is apparent that the described embodiments are only some embodiments of the present utility model, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Examples

As shown in fig. 1-8, a PCR plate for preventing cross infection, including a reaction plate 6, the reaction plate 6 cooperates and clamps on the upper surface of a lower supporting plate 2, a plurality of reaction cylinders 11 are arranged on the surface of the reaction plate 6, the upper surface of each reaction cylinder 11 is sealed by a film sealing plate 1, reaction cylinder clamping grooves 605 equal to the reaction cylinders 11 in number are arranged on the surface of the reaction plate 6, drainage grooves 606 are arranged between each row of opposite reaction cylinder clamping grooves 605, the drainage grooves 606 are arranged on the upper surface of the reaction plate 6, a skirt edge 12 is arranged on the side wall of the reaction plate 6, four lower fixing rods 503 are symmetrically arranged on the lower surface of the lower supporting plate 2 in a transverse central line, each lower fixing rod 503 slides in a short distance through a fine adjusting component 5, an upper drainage groove cover plate 101 equal to the reaction cylinders 11 in number is fixedly connected to the lower surface of the upper sealing plate 1, and the upper drainage groove cover plate 101 cooperates and clamps on the reaction cylinders 11.

According to the above technical scheme, firstly, each reaction cylinder 11 is placed on the reaction cylinder clamping groove 605, then, nutrient solution is poured into the reaction cylinder 11, then, the reaction plate 6 is placed on the lower supporting plate 2 for limiting and fixing, if the volume and caliber of the reaction cylinder are smaller when nutrient solution is dripped, the nutrient solution is easy to drop on the plate, cross infection can be caused when the nutrient solution is wiped, at this time, the knob 506 can be rotated, the worm wheel 502 and the worm 505 are matched to rotate, meanwhile, the screw rod sleeve and the screw rod 501 are matched, the lower fixing rod 503 is further moved downwards, the ball 504 arranged at the lower end in the moving process can stably support the reaction plate 6 when the reaction plate is deflected to one side, and the nutrient solution dripped on the reaction plate 6 can flow out through the drainage groove 606, so that the problem of cross infection caused by wiping is avoided.

Meanwhile, the edge sealing air bags 103 are arranged at the upper ends, the problem of cross infection inside the reaction cylinder 11 caused by air circulation is avoided by extruding the edge sealing air bags 103, the side edge of the film sealing plate 1 is firstly slidably mounted along the first clamping groove 601, when the film sealing plate 1 is clamped to the skirt edge 12, the film sealing plate 1 is forcefully pressed downwards, the pvc material is adopted by the film sealing plate 1 to have the elastic characteristic, two sides of the PVC material downwards move through the round slide bars 603, the round slide bars 603 are aligned for limiting, the traditional glue layer fixing is replaced, the problem of cross infection caused by later loosening is avoided, and stable conditions are provided for culturing PCR plate cells.

In this embodiment, the shape of the reaction plate 6 is rectangular, the skirt 12 is fixedly connected to the long sides of the reaction plate 6 and the wide sides of one side, the drainage groove 606 extends to the wide sides of the reaction plate 6 where the skirt 12 is not arranged, round holes 608 are formed in the outer wall of each reaction cylinder clamping groove 605, each round hole 608 is formed in the upper surface of the reaction plate 6, inclined planes 607 are formed in the inner wall of each round hole 608, each round hole 608 is matched and clamped with the upper reaction cylinder cover plate 104, firstly, each reaction cylinder 11 is placed on the reaction cylinder clamping groove 605, then nutrient solution is poured into the reaction cylinder 11, the reaction plate 6 is placed on the lower supporting plate 2 immediately, limiting fixation is performed, when the nutrient solution is dripped, if the volume and caliber of the reaction cylinder are small, the nutrient solution is easy to fall on the plate, cross infection can be caused when the knob 506 is wiped, the knob 506 can be rotated at this time, the screw rod sleeve and the screw rod 501 are matched and rotated through the matching of the worm 502 and the screw rod 501, further the lower fixing rod moves downwards, and the lower fixing rod 504 is provided with the ball 504, so that the reaction plate 6 can be stably offset to one side when the ball 6 is arranged in the moving process.

In this embodiment, the reaction cylinder upper cover plate 104 is fixedly connected to the lower surface of the film sealing plate 1, the edge sealing air bag 103 is fixedly connected to the outer wall of the reaction cylinder upper cover plate 104, the lower surface of the film sealing plate 1 is fixedly connected with the drainage groove upper cover plate 101, the end part of each drainage groove upper cover plate 101 is fixedly connected with the sealing plate 102, the fine adjustment assembly 5 comprises a worm wheel 502, a lower fixing rod 503, a worm 505 and a knob 506, the knob 506 is fixedly connected to one end of the worm 505, the worm 505 is rotatably connected to the inside of the lower supporting plate 2, the end part connected with the knob 506 is located outside the lower supporting plate 2, the worm 505 is in meshed connection with the worm wheel 502, the worm wheel 502 is arranged inside the lower supporting plate 2, so that cross infection can be caused when nutrient solution drops fall on the plates during wiping, the knob 506 can be rotated, the cooperation of the screw sleeve and the screw rod 501 can be moved downwards, the reaction plates 6 can be stably supported when the balls 504 arranged at the lower ends of the moving process can be shifted to one side, and the cross infection problem caused when the nutrient solution drops fall on the reaction plates 6 can be avoided through the guide rails 606.

In this embodiment, the inside of worm wheel 502 is equipped with the screw and screw 501 screw-thread fit, the upper and lower both ends of screw 501 are all fixedly connected with dead lever 503 down, be located the inner wall of dead lever 503 swing joint at bottom plate 2 under the upper end, be located the lower extreme fixedly connected with ball 504 of dead lever 503 under the lower extreme, horizontal groove 4 has been seted up to one side of bottom plate 2, the inside of horizontal groove 4 is settled there is spin 3, the upper surface fixedly connected with upper plate 9 of bottom plate 2, every upper plate 9 has been seted up and has been deposited hole 7, spacing hole 8 has been seted up to the upper surface of bottom plate 2, spacing hole 8 cooperation chucking has gag lever post 10, the banding gasbag 103 that the upper end was equipped with simultaneously, through the extrusion of banding gasbag 103, the problem of the inside cross infection of reaction cylinder 11 that causes because of the circulation of air has been avoided, at first sliding mounting along first chucking groove 601 with the side of sealing plate 1, when sealing plate 1 to shirt rim 12, press down forcefully, through sealing plate 1 adopts pvc material to have elasticity's characteristics, make its both sides move downwards through circular slide bar 603.

In this embodiment, the inner wall of the skirt 12 is provided with a U-shaped groove 602, a round bar 603 is fixedly connected in the middle of the U-shaped groove 602, a first clamping groove 601 is arranged at the upper end of the round bar 603, a second clamping groove 604 is arranged at the lower end of the round bar 603, and meanwhile, the upper end of the sealing air bag 103 is provided with the sealing air bag, so that the problem of cross infection inside the reaction cylinder 11 caused by air circulation is avoided by extrusion of the sealing air bag 103, the side edge of the sealing plate 1 is firstly slidably mounted along the first clamping groove 601, and when the sealing plate 1 is clamped to the skirt 12, the sealing plate 1 is pressed downwards with force, and the sealing plate 1 is made of pvc material with the elastic characteristic, so that two sides of the sealing plate move downwards through the round bar 603.

The working principle of the PCR plate for preventing cross infection is as follows: firstly, each reaction cylinder 11 is placed on a reaction cylinder clamping groove 605, then nutrient solution is poured into the reaction cylinder 11, then a reaction plate 6 is placed on a lower supporting plate 2 for limiting and fixing, when the nutrient solution is dripped, if the volume and caliber of the reaction cylinder are smaller, the nutrient solution drops onto the plate easily and cross infection can be caused when the nutrient solution drops onto the plate and is wiped, at this time, a knob 506 can be rotated, the rotation is matched with a turbine 502 and a worm 505, meanwhile, a screw rod sleeve is matched with a screw rod 501, a lower fixing rod 503 is further moved downwards, a ball 504 arranged at the lower end in the moving process can stably support the reaction plate 6 when the reaction plate is deflected to one side, and the nutrient solution dripped onto the reaction plate 6 flows out through a drainage groove 606, so that the problem of cross infection caused by wiping is avoided.

The foregoing has shown and described the basic principles and main features of the present utility model and the advantages of the present utility model. It will be understood by those skilled in the art that the present utility model is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present utility model, and various changes and modifications may be made without departing from the spirit and scope of the utility model, which is defined in the appended claims. The scope of the utility model is defined by the appended claims and equivalents thereof.

Claims

1. A PCR plate for preventing cross-infection comprising a reaction plate (6), characterized in that: the reaction plate (6) cooperation chucking is at the upper surface of bed board (2), the surface of reaction plate (6) is provided with a plurality of reaction cylinder (11), every the upper surface of reaction cylinder (11) is sealed through seal plate (1), reaction cylinder draw-in groove (605) that equals with reaction cylinder (11) quantity are seted up on the surface of reaction plate (6), every relative be listed as be provided with drainage groove (606) between reaction cylinder draw-in groove (605), just drainage groove (606) are offered at the upper surface of reaction plate (6), the limit wall of reaction plate (6) is provided with shirt rim (12), the lower surface of bed board (2) is provided with four lower dead lever (503) with lateral center line symmetry, and every lower dead lever (503) slide from top to bottom through fine setting subassembly (5), the lower surface of seal plate (1) still fixedly connected with and the drainage groove upper cover plate (101) that equals with reaction cylinder (11) quantity, drainage groove (101) cooperate chucking on reaction plate (11) and be on reaction plate (6), reaction plate (6) are on the rectangle side of side and side of extension (6), reaction plate (6) are on one side of reaction plate (6) and side extension board (12).

2. A cross-infection resistant PCR plate according to claim 1, wherein: the outer wall of each reaction needle cylinder clamping groove (605) is provided with a round hole (608), and each round hole (608) is formed in the upper surface of the reaction plate (6).

3. A cross-infection resistant PCR plate according to claim 2, wherein: the inner wall of each round hole (608) is provided with an inclined surface (607), and each round hole (608) is matched and clamped with an upper cover plate (104) of the reaction cylinder.

4. A cross-infection resistant PCR plate according to claim 3, wherein: the upper cover plate (104) of the reaction cylinder is fixedly connected to the lower surface of the film sealing plate (1), and the outer wall of the upper cover plate (104) of the reaction cylinder is fixedly connected with an edge sealing air bag (103).

5. The cross-infection resistant PCR plate of claim 4 wherein: and the lower surface of the film sealing plate (1) is fixedly connected with drainage groove upper cover plates (101), and the end part of each drainage groove upper cover plate (101) is fixedly connected with a sealing plate (102).

6. A cross-infection resistant PCR plate according to claim 1, wherein: the fine adjustment assembly (5) comprises a worm wheel (502), a lower fixing rod (503), a worm (505) and a knob (506), wherein the knob (506) is fixedly connected to one end of the worm (505), the worm (505) is rotationally connected to the inside of the lower support plate (2), the end part connected with the knob (506) is located on the outside of the lower support plate (2), the worm (505) is connected with the worm wheel (502) in a matched mode, and the worm wheel (502) is arranged inside the lower support plate (2).

7. The cross-infection resistant PCR plate of claim 6 wherein: the inside of worm wheel (502) is equipped with screw and screw (501) screw thread fit, the upper and lower both ends of screw (501) are all fixedly connected with down dead lever (503), are located the upper end down dead lever (503) swing joint is at the inner wall of bottom plate (2), is located the lower extreme fixedly connected with ball (504) of lower dead lever (503).

8. A cross-infection resistant PCR plate according to claim 1, wherein: horizontal groove (4) have been seted up to one side of bottom board (2), the internally mounted of horizontal groove (4) has spin (3), the last fixed surface of bottom board (2) is connected with upper plate (9), every upper plate (9) have been seted up and have been deposited hole (7), spacing hole (8) have been seted up to the upper surface of bottom board (2), spacing hole (8) cooperation chucking has gag lever post (10).

9. A cross-infection resistant PCR plate according to claim 1, wherein: u-shaped grooves (602) are formed in the inner wall of the skirt edge (12), round rods (603) are fixedly connected to the middle of the U-shaped grooves (602), the upper ends of the round rods (603) are provided with first clamping grooves (601), and the lower ends of the round rods (603) are provided with second clamping grooves (604).