CN220665346U - Cell culture is with many orbit oscillation equipment - Google Patents
Cell culture is with many orbit oscillation equipment Download PDFInfo
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- CN220665346U CN220665346U CN202322262923.1U CN202322262923U CN220665346U CN 220665346 U CN220665346 U CN 220665346U CN 202322262923 U CN202322262923 U CN 202322262923U CN 220665346 U CN220665346 U CN 220665346U
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- 238000004113 cell culture Methods 0.000 title claims abstract description 54
- 230000010355 oscillation Effects 0.000 title claims description 13
- 230000000149 penetrating effect Effects 0.000 claims description 2
- 239000007788 liquid Substances 0.000 description 11
- 238000001125 extrusion Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 238000010367 cloning Methods 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000004500 asepsis Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000010370 cell cloning Methods 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000009827 uniform distribution Methods 0.000 description 1
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Abstract
The utility model discloses a multi-track oscillating device for cell culture, which comprises an oscillating device body, a shaking component for transversely oscillating a cell culture carrier and a pushing component for longitudinally oscillating the cell culture carrier, wherein the shaking component comprises a fixed frame arranged at the top of the body, a sliding frame arranged at the inner side of the fixed frame for oscillating and three pushing frames respectively arranged at two sides of the inner part of the sliding frame and used for extruding.
Description
Technical Field
The utility model relates to the technical field of cell culture, in particular to a multi-track oscillating device for cell culture.
Background
The cell culture refers to a method for simulating in vitro an in vivo environment (asepsis, proper temperature, pH value, a certain nutrition condition and the like) to enable the cells to survive, grow, reproduce and maintain main structures and functions, the cell culture is also called a cell cloning technology, the normal noun in biology is a cell culture technology, no matter for the whole bioengineering technology or one of the biological cloning technologies, the cell culture is an essential process, the cell culture technology can be a large-scale cloning of cells, the cell culture technology can be a simple single cell or a few differentiated multicellular by a large number of cells through a large number of culture, the cell culture technology is a cloning of the cells, the cell culture technology is an important and common technology in a cell biology research method, a large number of cells can be obtained through the cell culture, the signal transduction of the cells, the synthesis metabolism of the cells, the growth proliferation of the cells and the like can be researched, in the cell culture process, a test tube filled with a cell culture liquid is usually oscillated by a cell culture oscillator, the oscillating device is usually a single cell culture oscillation device, the single cell rate is influenced in a single direction, the cell culture is influenced,
the current equipment is the unidirectional vibration usually when carrying out the vibration to cell culture liquid test tube, can not carry out multidirectional vibration to the culture test tube, leads to the nutrient composition of culture liquid to distribute unevenly easily, reduces cell culture's growth rate, and can not be convenient carry out stable centre gripping to cell culture carrier fixedly, influence the effect and the practicality that the equipment carries out the vibration to cell culture liquid.
Disclosure of Invention
The utility model aims to provide a multi-track oscillating device for cell culture, which solves the problems of the prior art.
In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides a cell culture is with many tracks oscillation device, including oscillation device's body and be used for carrying out horizontal oscillating rocking part and be used for carrying out vertical oscillating pushing component to cell culture carrier, rocking part is including locating the mount that is used for placing at the body top, locate the carriage that is used for oscillating in the mount, locate the inside both sides of carriage respectively and evenly arrange and be used for extruded three pushing frames, locate the grip block that is used for centre gripping in pushing frame one side, locate the pull rod that is used for pulling in pushing frame, locate the connecting plate that a plurality of pull rods one end are used for pulling, locate the test-tube rack that is located in the carriage and is used for fixing the cell culture test tube between two grip blocks, offer the movable through-hole that is used for providing the activity space in mount one side, run through the dwang that is used for driving in the mount opposite side, the cam that the cover is located the dwang outside, locate the mount opposite side and be located the motor of dwang one end, locate a plurality of guide bars that the inside opposite side of mount evenly arrange, cover locates the first spring that the guide bar outside is used for extrusion and locate a plurality of first spring one end that the first spring is used for oscillating;
the test tube rack is movably connected with the fixing frame, the pull rod penetrates through the pushing frame and is fixedly connected with the pushing frame, the pull rod is fixedly connected with the sliding frame, one end of the first spring is fixedly connected with the sliding frame, the other end of the first spring is fixedly connected with the pushing plate, and the rotating rod is rotationally connected with the cam.
Preferably, the pushing component comprises two air cylinders arranged on the other side of the fixing frame and used for providing power, a push rod arranged on one side of the air cylinders, a base plate arranged on one end of the push rod and used for being attached to the sliding frame, an elastic frame arranged on the other side of the fixing frame and a fixing plate arranged on one side of the elastic frame and used for pushing the sliding frame, the elastic frame is fixedly connected with the fixing plate, and the push rod is fixedly connected with the base plate.
Preferably, the two sides of the sliding frame are respectively provided with a limiting rod used for guiding, the two sides of the fixing frame are respectively provided with a limiting through hole, and the limiting rods are in sliding connection with the limiting through holes.
Preferably, a second spring is sleeved on one side of the pushing frame and positioned on the outer side of the pull rod, and the second spring is fixedly connected with the pushing frame.
Preferably, a fixed block used for supporting is arranged on one side of the elastic frame, a movable cavity is formed in the inner side of the fixed block, an elastic piece is arranged at the bottom of the movable cavity, a supporting rod used for pushing is arranged on one side of the elastic piece, the section of the supporting rod is H-shaped, the supporting rod is in sliding connection with the fixed block, and the section of the elastic piece is Z-shaped.
Preferably, a third spring is sleeved on one side of the fixed block and positioned on the outer side of the supporting rod.
Preferably, a limiting block is arranged on one side of the clamping plate, and the cross section of the limiting block is triangular.
Compared with the prior art, the utility model has the beneficial effects that:
1. according to the utility model, the body, the fixing frame, the sliding frame, the pushing frame, the clamping plate, the pull rod, the connecting plate, the test tube rack, the movable through hole, the rotating rod, the cam, the motor, the guide rod, the first spring and the pushing plate are movably connected with the fixing frame, the test tube rack is movably connected with the sliding frame, the pull rod penetrates through the pushing frame and is fixedly connected with the pushing frame, the pull rod is fixedly connected with the sliding frame, one end of the first spring is fixedly connected with the pushing plate, the rotating rod is rotatably connected with the cam, the connecting plate can pull the pushing frame through the pull rod, the test tube rack is convenient to place and take out, the clamping plate is used for clamping and fixing the test tube rack through the plurality of pushing frames, the cam on the outer side of the rotating rod is driven by the motor to rotate, then the cam extrudes and pushes the sliding frame, then the sliding frame transversely moves, then the pushing plate on one end of the first spring is extruded, then the first spring stretches and extrudes the sliding frame after the cam end is separated from the sliding frame, and then the pushing plate transversely moves, so that the sliding frame drives the cell culture test tube in the rack to transversely oscillate, the test tube, the stability of the fixed test tube rack is driven, and the cell culture liquid is transversely oscillated.
2. According to the utility model, the air cylinder, the push rod, the base plate, the elastic frame and the fixing plate are fixedly connected, the push rod is fixedly connected with the base plate, the air cylinder can push the base plate through the push rod, the base plate longitudinally extrudes and pushes the sliding frame, the sliding frame extrudes the elastic frame on one side of the fixing plate, the push rod drives the base plate to rapidly separate from the sliding frame, and the elastic frame pushes the sliding frame through the fixing plate, so that the sliding frame can drive the cell culture liquid to longitudinally oscillate, the effect and the practicability of the device for multidirectional oscillation of the cell culture liquid are improved, the uniform distribution of nutrient components in the cell culture liquid can be prevented, and the growth rate of cell culture is improved.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present utility model;
FIG. 2 is a left side cutaway perspective view of the overall structure of the present utility model;
FIG. 3 is a front cross-sectional perspective view of the overall structure of the present utility model;
FIG. 4 is an enlarged view of the entire structure of the present utility model at A in FIG. 2;
FIG. 5 is an enlarged view of the entire structure of the present utility model at B in FIG. 2;
fig. 6 is an enlarged view of fig. 3C of the overall structure of the present utility model.
In the figure: 1. a body; 11. a fixing frame; 12. a carriage; 13. a pushing frame; 14. a clamping plate; 15. a pull rod; 16. a connecting plate; 17. a test tube rack; 18. a movable through hole; 19. a rotating lever; 20. a cam; 21. a motor; 22. a guide rod; 23. a first spring; 24. a push plate; 25. a cylinder; 26. a push rod; 27. a backing plate; 28. an elastic frame; 29. a fixing plate; 30. a limit rod; 31. limiting through holes; 32. a second spring; 33. a fixed block; 34. a movable cavity; 35. a spring plate; 36. a support rod; 37. a third spring; 38. and a limiting block.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
For a further understanding of the utility model, its features and advantages, reference is now made to the following examples, which are illustrated in the accompanying drawings.
The structure of the present utility model will be described in detail with reference to the accompanying drawings.
As shown in fig. 1 to 6, the multi-track oscillation device for cell culture provided by the embodiment of the utility model comprises a body 1 of the oscillation device, a shaking component for horizontally oscillating a cell culture carrier and a pushing component for longitudinally oscillating the cell culture carrier, wherein the shaking component comprises a fixed frame 11 arranged at the top of the body 1, a sliding frame 12 arranged at the inner side of the fixed frame 11 and used for oscillating, three pushing frames 13 respectively arranged at two sides of the inner side of the sliding frame 12 and used for extrusion, a clamping plate 14 arranged at one side of the pushing frame 13 and used for clamping, a pull rod 15 arranged at the inner side of the pushing frame 13, a connecting plate 16 arranged at one end of the plurality of pull rods 15 and used for pulling, a test tube rack 17 arranged at the inner side of the sliding frame 12 and positioned between the two clamping plates 14 and used for fixing a cell culture test tube, a movable through hole 18 arranged at one side of the fixed frame 11 and used for providing a movable space, a rotating rod 19 penetrating through the other side of the fixed frame 11 and used for driving, a cam 20 sleeved at the outer side of the rotating rod 19, a motor 21 arranged at the other side of the fixed frame 19, a plurality of guide rods 22 uniformly arranged at the other side of the inner side of the fixed frame 11 and used for extrusion, a plurality of springs 23 sleeved at the outer side of the first ends of the guide rods 22 and used for extrusion springs 23 arranged at the outer side of the first ends of the springs;
the test tube rack is characterized in that the sliding frame 12 is movably connected with the fixed frame 11, the test tube rack 17 is movably connected with the sliding frame 12, the pull rod 15 penetrates through the pushing frame 13 and is fixedly connected with the pushing frame 13, the pull rod 15 is fixedly connected with the sliding frame 12, one end of the first spring 23 is fixedly connected with the sliding frame 12, the other end of the first spring 23 is fixedly connected with the push plate 24, and the rotating rod 19 is rotatably connected with the cam 20.
Referring to fig. 3 and 6, the pushing component includes two cylinders 25 disposed on the other side of the fixed frame 11 for providing power, a push rod 26 disposed on one side of the cylinders 25, a pad 27 disposed on one end of the push rod 26 for adhering to the sliding frame 12, an elastic frame 28 disposed on the other side of the fixed frame 11, and a fixing plate 29 disposed on one side of the elastic frame 28 for pushing the sliding frame 12, wherein the elastic frame 28 is fixedly connected with the fixing plate 29, and the push rod 26 is fixedly connected with the pad 27.
The scheme is adopted: through cylinder 25, push rod 26, backing plate 27, elastic support 28 and fixed plate 29 fixed connection, push rod 26 and backing plate 27 fixed connection, cylinder 25 can promote backing plate 27 through push rod 26, then backing plate 27 carries out vertical extrusion to carriage 12 and promotes, then carriage 12 extrudees elastic support 28 of fixed plate 29 one side, then push rod 26 drives backing plate 27 and breaks away from carriage 12 fast, then elastic support 28 promotes carriage 12 through fixed plate 29, thereby can make carriage 12 drive the cell culture liquid and carry out vertical oscillation, thereby improve effect and the practicality that the equipment carries out multi-direction oscillation to the cell culture liquid, can prevent the nutrient composition evenly distributed in the cell culture liquid, improve the growth rate of cell culture.
Referring to fig. 1, two sides of the sliding frame 12 are respectively provided with a limiting rod 30 for guiding, two sides of the fixing frame 11 are respectively provided with a limiting through hole 31, and the limiting rods 30 are slidably connected with the limiting through holes 31.
The scheme is adopted: through gag lever post 30 and spacing through-hole 31, gag lever post 30 and spacing through-hole 31 sliding connection, carriage 12 drive gag lever post 30 and transversely and longitudinally move in spacing through-hole 31 when taking place to slide, can make two gag lever posts 30 lead and spacing carriage 12, prevent that carriage 12 from taking place to shift or break away from.
Referring to fig. 4, a second spring 32 is sleeved on one side of the pushing frame 13 and located outside the pull rod 15, and the second spring 32 is fixedly connected with the pushing frame 13.
The scheme is adopted: through the second spring 32, second spring 32 and pushing frame 13 fixed connection, second spring 32 can extrude pushing frame 13, then pushes pushing frame 13 extrudees the push pedal 24 and promotes pushing frame 13 and promote the intensity that pushing frame 13 promoted carriage 12 and remove.
Referring to fig. 6, a fixed block 33 for supporting is provided on one side of the elastic frame 28, a movable cavity 34 is provided on the inner side of the fixed block 33, a spring plate 35 is provided at the bottom of the movable cavity 34, a supporting rod 36 for pushing is provided on one side of the spring plate 35, the cross section of the supporting rod 36 is H-shaped, the supporting rod 36 is slidably connected with the fixed block 33, and the cross section of the spring plate 35 is Z-shaped.
The scheme is adopted: through fixed block 33, movable cavity 34, shell fragment 35 and bracing piece 36, bracing piece 36 cross-section is the H font, bracing piece 36 and fixed block 33 sliding connection, and the shell fragment 35 cross-section is the Z font, and elastic support 28 receives the extrusion and takes place the compression, because the effect is mutual, and shell fragment 35 in the fixed block 33 can extrude bracing piece 36, then bracing piece 36 can support elastic support 28, improves elastic support 28 and promotes the intensity that the carriage 12 moved.
Referring to fig. 6, a third spring 37 is sleeved on one side of the fixed block 33 and outside the supporting rod 36.
The scheme is adopted: the third spring 37 can push the support rod 36 in a squeezing manner through the third spring 37, so that the strength of pushing the elastic frame 28 by the support rod 36 is improved.
Referring to fig. 4, a limiting block 38 is disposed on one side of the clamping plate 14, and a cross section of the limiting block 38 is triangular.
The scheme is adopted: through stopper 38, stopper 38 cross-section is triangle-shaped, can push down spacing to test-tube rack 17, prevents that test-tube rack 17 from taking place to shift or break away from.
The working principle of the utility model is as follows:
firstly, the connecting plate 16 is pulled to push the rack 13 through the pull rod 15, then the test tube rack 17 is placed between the two clamping plates 14, then the connecting plate 16 is slowly released, at this time, the plurality of push racks 13 extrude the clamping plates 14 to clamp and fix the test tube rack 17, meanwhile, the second spring 32 extrudes the push rack 13, at this time, the clamping plates 14 drive the limiting block 38 to limit the test tube rack 17, then the motor 21 is started, the cam 20 outside the rotating rod 19 is driven to rotate, then the cam 20 extrudes and pushes the sliding rack 12, then the sliding rack 12 transversely moves, then the sliding rack 12 extrudes the push plate 24 at one end of the first spring 23, after the end part of the cam 20 is separated from the sliding rack 12, the first spring 23 stretches to extrude the push plate 24, then the push plate 24 extrudes the sliding rack 12 transversely, so that the sliding rack 12 drives the cell culture test tube in the test tube rack 17, then the cylinder 25 is started, so that the sliding rack 27 pushes the backing plate 27, then the backing plate 27 longitudinally extrudes and pushes the sliding rack 12, then the sliding rack 12 extrudes the elastic rack 28 at one side of the fixed plate 29, then the push rod 26 drives the backing plate 26 to extrude the backing plate 28 to move the elastic rack 36, and simultaneously, and the elastic support rod 36 is driven by the elastic support rod 36 is longitudinally moves the elastic support rod 36, and simultaneously, and the elastic support rod 36 is driven by the elastic support rod 36 is transversely to longitudinally move the elastic support rod 36, at this time, and the elastic support rod is simultaneously, the elastic support rod is driven by the backing rod 12 is pushed by the backing rod 12 and the backing plate 12.
What is not described in detail in this specification is prior art known to those skilled in the art.
Although the present utility model has been described with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described, or equivalents may be substituted for elements thereof, and any modifications, equivalents, improvements and changes may be made without departing from the spirit and principles of the present utility model.
Claims (7)
1. The utility model provides a cell culture is with many tracks oscillation device, includes oscillation device's body (1) and is used for carrying out horizontal oscillating rocking part and be used for the promotion part to cell culture carrier longitudinal oscillation to cell culture carrier, its characterized in that: the shaking component comprises a fixing frame (11) arranged at the top of the body (1), a sliding frame (12) arranged at the inner side of the fixing frame (11) and used for oscillating, three pushing frames (13) which are respectively arranged at the two sides of the inner part of the sliding frame (12) and used for extruding, a clamping plate (14) arranged at one side of the pushing frames (13) and used for clamping, a pull rod (15) arranged at the inner side of the pushing frames (13), a connecting plate (16) arranged at one end of the plurality of pull rods (15) and used for pulling, a test tube rack (17) arranged at the inner side of the sliding frame (12) and positioned between the two clamping plates (14) and used for fixing a cell culture test tube, a movable through hole (18) arranged at one side of the fixing frame (11) and used for providing a movable space, a rotating rod (19) penetrating through the other side of the fixing frame (11) and used for driving, a cam (20) sleeved at the outer side of the rotating rod (19), a motor (21) arranged at the other side of the fixing frame (11) and positioned at one end of the rotating rod (19), a plurality of guide rods (22) uniformly arranged at the other side of the inner part of the fixing frame (11), a plurality of springs (23) sleeved at the outer side of the fixing rod and used for extruding, and a plurality of springs (23) arranged at one end and used for oscillating;
the test tube rack is characterized in that the sliding frame (12) is movably connected with the fixing frame (11), the test tube rack (17) is movably connected with the sliding frame (12), the pull rod (15) penetrates through the pushing frame (13) and is fixedly connected with the pushing frame, the pull rod (15) is fixedly connected with the sliding frame (12), one end of the first spring (23) is fixedly connected with the sliding frame (12), the other end of the first spring (23) is fixedly connected with the pushing plate (24), and the rotating rod (19) is rotatably connected with the cam (20).
2. The multi-track shaking apparatus for cell culture according to claim 1, wherein: the pushing component comprises two air cylinders (25) arranged on the other side of the fixed frame (11) and used for providing power, a push rod (26) arranged on one side of the air cylinders (25), a base plate (27) arranged on one end of the push rod (26) and used for being attached to the sliding frame (12), an elastic frame (28) arranged on the other side of the fixed frame (11) and a fixed plate (29) arranged on one side of the elastic frame (28) and used for pushing the sliding frame (12), the elastic frame (28) is fixedly connected with the fixed plate (29), and the push rod (26) is fixedly connected with the base plate (27).
3. The multi-track shaking apparatus for cell culture according to claim 1, wherein: limiting rods (30) used for guiding are respectively arranged on two sides of the sliding frame (12), limiting through holes (31) are respectively formed in two sides of the fixing frame (11), and the limiting rods (30) are in sliding connection with the limiting through holes (31).
4. The multi-track shaking apparatus for cell culture according to claim 1, wherein: the pushing frame (13) is provided with a second spring (32) in a sleeved mode on one side of the pushing frame (13) and located on the outer side of the pull rod (15), and the second spring (32) is fixedly connected with the pushing frame (13).
5. The multi-track shaking apparatus for cell culture according to claim 2, wherein: the spring frame is characterized in that a fixed block (33) used for supporting is arranged on one side of the spring frame (28), a movable cavity (34) is formed in the inner side of the fixed block (33), an elastic piece (35) is arranged at the bottom of the movable cavity (34), a supporting rod (36) used for pushing is arranged on one side of the elastic piece (35), the cross section of the supporting rod (36) is H-shaped, the supporting rod (36) is in sliding connection with the fixed block (33), and the cross section of the elastic piece (35) is Z-shaped.
6. The multi-track shaking apparatus for cell culture according to claim 5, wherein: and a third spring (37) is sleeved on one side of the fixed block (33) and positioned outside the supporting rod (36).
7. The multi-track shaking apparatus for cell culture according to claim 1, wherein: a limiting block (38) is arranged on one side of the clamping plate (14), and the cross section of the limiting block (38) is triangular.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322262923.1U CN220665346U (en) | 2023-08-22 | 2023-08-22 | Cell culture is with many orbit oscillation equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322262923.1U CN220665346U (en) | 2023-08-22 | 2023-08-22 | Cell culture is with many orbit oscillation equipment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220665346U true CN220665346U (en) | 2024-03-26 |
Family
ID=90344427
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322262923.1U Active CN220665346U (en) | 2023-08-22 | 2023-08-22 | Cell culture is with many orbit oscillation equipment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220665346U (en) |
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2023
- 2023-08-22 CN CN202322262923.1U patent/CN220665346U/en active Active
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