WO2023093877A1

WO2023093877A1 - Pcr plate for preventing cross infection

Info

Publication number: WO2023093877A1
Application number: PCT/CN2022/134594
Authority: WO
Inventors: 刘子贤; 刘刚; 刘子德; 袁义勇; 赵华; 唐波
Original assignee: 美东汇成生命科技(昆山)有限公司
Priority date: 2021-11-28
Filing date: 2022-11-28
Publication date: 2023-06-01
Also published as: CN114214169B; CN114214169A

Abstract

A PCR plate for preventing cross infection, the plate comprising a reaction plate, wherein the reaction plate is clamped to the upper surface of a lower supporting plate in a fitted manner; a surface of the reaction plate is provided with several reaction needle cylinders; the upper surface of each reaction needle cylinder is sealed by a film sealing plate; the surface of the reaction plate is provided with reaction needle cylinder clamping grooves corresponding to the reaction needle cylinders in number; a drainage groove is arranged between two rows of reaction needle cylinder clamping grooves opposite each other and provided on the upper surface of the reaction plate; an edge wall of the reaction plate is provided with a skirt edge; the lower surface of an upper film sealing plate is also fixedly connected to drainage groove upper cover plates corresponding to the reaction needle cylinders in number; and the drainage groove upper cover plates are clamped to the reaction needle cylinders in a fitted manner.

Description

A kind of anti-cross-infection PCR plate

technical field

The invention relates to the technical field of PCR plates, in particular to a PCR plate for preventing cross-infection.

Background technique

The PCR plate is a carrier that is mainly used as primers, buffers, etc. for the amplification reaction in the polymerase chain reaction. It is widely used in PCR laboratories. When PCR culture cells for scientific research, in order to prevent adjacent The nutrient solution in the reaction tube is mixed into it, causing cross-infection. Usually, the top of the reaction tube is covered with a sealing plate, thereby reducing the hidden danger of cross-infection. It has been widely used in PCR laboratories, such as application number CN201720555830. X, a PCR plate with a PCR cover, comprising a PCR plate body and two PCR covers, both PCR covers are movably fixed on the PCR plate body; the PCR plate body is provided with a reaction tube, and the PCR plate Hollow tubes are fixed on both sides of the body, and the two hollow tubes are less than half the length of the PCR plate body and are located at both ends of the PCR plate body; both ends of the PCR plate body are provided with fixing rings; the overall PCR cover is transparent , and the PCR cover is provided with a plurality of rings, and the bottom surface of each ring is located on the same horizontal plane; the PCR cover is provided with a rotating shaft on one side, and a fixed buckle is provided on the other side of the PCR cover. The utility model can seal each reaction tube, and can separate each reaction tube, which improves the experimental accuracy of the PCR reaction, brings convenience to the operation of the PCR reaction, and can be sealed while being sealed. The experiments in the reaction tubes are observed in real time, the PCR plate body and the PCR cover hole can be disassembled, and the PCR cover has a good seal for each reaction tube.

technical problem

However, the above-mentioned solution has the following defects in use: the above-mentioned application device only uses a simple ring for sealing, but the sealing structure is simple, and it is easy to loosen during the process of statically culturing the internal cells, and then cause cross-infection through air flow. At the same time, when the nutrient solution is dripped into the reaction cylinder, due to the small volume and caliber inside the reaction cylinder, it is easy to make the nutrient solution drop onto the plate and cause cross infection when wiping.

technical solution

In view of the prior art, only a simple ring is used for sealing, but the sealing structure is simple, and it is easy to loosen during the process of statically culturing the internal cells, and then cause cross-infection through air flow. The present invention provides an anti- For cross-infected PCR plates, due to the small volume and caliber inside the reaction cylinder, it is easy for the nutrient solution to drop onto the plate, which will also cause cross-infection when wiping. In order to achieve the above object, the technical scheme that the present invention takes is:

A PCR plate for preventing cross-infection, including a reaction plate, the reaction plate is fastened on the upper surface of the lower supporting plate, the surface of the reaction plate is provided with several reaction syringes, and the upper surface of each reaction syringe is The surface is sealed by a sealing film plate, and the surface of the reaction plate is provided with reaction syringe slots equal to the number of reaction syringes, and drainage grooves are arranged between the reaction syringe slots in each row, and the The drainage groove is set on the upper surface of the reaction plate, the side wall of the reaction plate is provided with a skirt, and the lower surface of the lower supporting plate is symmetrically provided with four lower fixing rods on the transverse center line, and each of the lower fixing rods The rod slides for a short distance through the fine-tuning assembly. The lower surface of the upper sealing film plate is also fixedly connected with the upper cover of the drainage groove equal to the number of the reaction syringes, and the upper cover of the drainage groove is matched and clamped on the reaction syringe. superior.

Preferably, the shape of the reaction plate is rectangular, and the skirt is fixedly connected to two long sides and one wide side of the reaction plate, and the drainage groove extends to one wide side of the reaction plate.

Preferably, the outer wall of each of the reaction syringe slots is provided with a circular hole, and each of the circular holes is opened on the upper surface of the reaction plate.

Preferably, the inner wall of each of the circular holes is provided with a slope, and each of the circular holes is matched with an upper cover plate of the reaction syringe.

Preferably, the upper cover of the reaction syringe is fixedly connected to the lower surface of the sealing film plate, and the outer wall of the upper cover of the reaction syringe is fixedly connected with an edge-sealing airbag.

Preferably, the lower surface of the sealing film plate is fixedly connected with the upper cover plate of the drainage groove, and the end of each upper cover plate of the drainage groove is fixedly connected with the sealing plate.

Preferably, the fine-tuning assembly includes a worm wheel, a lower fixed rod, a worm, and a knob, the knob is fixedly connected to one end of the worm, the worm is rotatably connected to the inside of the lower pallet and the end connected to the knob is located at the bottom of the lower pallet. Externally, the worm is engaged with the worm gear, and the worm gear is arranged inside the lower supporting plate.

As a preference, the inside of the worm wheel is provided with a screw hole and the screw hole is threadedly matched with the screw rod, the upper and lower ends of the screw rod are fixedly connected with a lower fixing rod, and the lower fixing rod at the upper end is movably connected to the bottom of the lower supporting plate. The inner wall is fixedly connected with a ball at the lower end of the lower fixed rod at the lower end.

As a preference, a horizontal groove is opened on one side of the lower supporting plate, rolling balls are placed inside the horizontal groove, an upper plate is fixedly connected to the upper surface of the lower supporting plate, each of the upper plates is provided with a storage The inner hole, the upper surface of the lower supporting plate is provided with a limit hole, and the limit hole cooperates with the clamping limit rod.

As a preference, a U-shaped groove is provided on the inner wall of the skirt, and a round rod is fixedly connected in the middle of the U-shaped groove. The upper end of the round rod is set as the first clamping groove, and the lower end of the round rod Set as the second clamping groove.

Beneficial effect

Compared with the prior art, the present invention has the following beneficial effects:

1. First place each reaction syringe on the reaction syringe slot, then pour the nutrient solution into the inside of the reaction syringe, then place the reaction plate on the lower support plate, and fix it in a limited position. When entering the nutrient solution, if there is a small volume and caliber inside the reaction cylinder, it is easy to make the nutrient solution drip onto the plate and cause cross-infection when wiping. At this time, you can turn the knob to rotate through the cooperation of the turbine and the worm At the same time, the cooperation between the screw sleeve and the screw rod makes the lower fixed rod move downward. During the movement, the ball at the lower end can make the reaction plate stable when it is shifted to one side, so that The nutrient solution dripped on the reaction plate flows out through the drainage groove, thereby avoiding the problem of cross-infection caused by wiping.

2. At the same time, the edge-sealing airbag on the upper end can avoid the problem of cross-infection inside the reaction syringe caused by air circulation through the extrusion of the edge-sealing airbag. First, slide the side of the sealing film plate along the first clamping groove For installation, when the sealing plate is fastened to the skirt, press down hard, and the sealing plate is made of pvc material with elastic characteristics, so that both sides can move downward through the round rod, and the position can be limited by aligning the round rod. It replaces the traditional glue layer fixation, avoids the problem of cross-infection caused by later loosening, and provides stable conditions for the culture of PCR plate cells.

Description of drawings

Fig. 1 is the structural representation of a kind of anti-cross-infection PCR plate of the present invention;

Fig. 2 is a schematic structural view of a sealing film plate, a reaction plate, and a lower supporting plate of a PCR plate for preventing cross-infection of the present invention;

Fig. 3 is the structural schematic diagram of looking up of a sealing film plate, a reaction plate, and a lower supporting plate of a kind of anti-cross-infection PCR plate of the present invention;

Fig. 4 is the schematic structural representation enlarged at B place in Fig. 3 of a kind of anti-cross-infection PCR plate of the present invention;

Fig. 5 is the schematic structural representation enlarged at A place in Fig. 2 of a kind of anti-cross-infection PCR plate of the present invention;

Fig. 6 is the structural representation of the reaction plate of a kind of anti-cross-infection PCR plate of the present invention;

Fig. 7 is a schematic structural view of a fine-tuning assembly of a PCR plate for preventing cross-infection of the present invention;

Fig. 8 is a schematic diagram showing the enlarged structure at C in Fig. 6 of a cross-infection prevention PCR plate of the present invention.

In the figure: 1, sealing film plate; 101, upper cover plate of drainage tank; 102, sealing plate; 103, edge sealing air bag; 104, upper cover plate of reaction syringe; 2, lower supporting plate; 3, rolling ball; 4, Horizontal groove; 5, fine-tuning assembly; 501, screw rod; 502, worm gear; 503, lower fixing rod; 504, ball; 505, worm; 506, knob; 6, reaction plate; 601, first clamping groove; , U-shaped groove; 603, round rod; 604, second clamping groove; 605, reaction syringe clamping groove; 606, drainage groove; 607, inclined plane; 608, round hole; 7, storage inner hole; 8, limit Hole; 9, upper plate; 10, limit rod; 11, reaction syringe; 12, skirt.

Embodiments of the present invention

The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

Example

As shown in Figure 1-8, a PCR plate for preventing cross-infection includes a reaction plate 6, the reaction plate 6 is fastened on the upper surface of the lower supporting plate 2, and the surface of the reaction plate 6 is provided with several reaction syringes 11, The upper surface of each reaction syringe 11 is sealed by the sealing film plate 1, and the surface of the reaction plate 6 is provided with reaction syringe slots 605 equal in number to the reaction syringes 11, and each row of reaction syringe slots 605 is opposite There is a drainage groove 606 between them, and the drainage groove 606 is set on the upper surface of the reaction plate 6, the side wall of the reaction plate 6 is provided with a skirt 12, and the lower surface of the lower supporting plate 2 is symmetrically arranged with four lower fixing plates on the transverse center line. Rods 503, and each lower fixed rod 503 slides for a short distance through the fine-tuning assembly 5, and the lower surface of the upper sealing film plate 1 is also fixedly connected with the upper cover plate 101 of the drainage groove equal to the number of the reaction syringes 11, and the upper surface of the drainage groove The cover plate 101 is matched and clamped on the reaction syringe 11 .

According to the above technical solution, first place each reaction syringe 11 on the reaction syringe slot 605, then pour the nutrient solution into the inside of the reaction syringe 11, and then place the reaction plate 6 on the lower supporting plate 2 , to fix the limit. When dropping the nutrient solution, if there is a small volume and caliber inside the reaction cylinder, it is easy to make the nutrient solution drop onto the plate. When wiping, it will also cause cross-infection. At this time, you can turn the knob 506, through the cooperative rotation of the turbine 502 and the worm screw 505, and the cooperation of the screw sleeve and the screw mandrel 501 at the same time, the lower fixed rod 503 is moved downward, and the ball 504 provided at the lower end can make the reaction plate 6 move downward during the moving process. Stable support can be provided when shifting to one side, so that the nutrient solution dripping on the reaction plate 6 can flow out through the drainage groove 606, thereby avoiding the problem of cross-infection caused by wiping.

At the same time, the edge-sealing airbag 103 provided on the upper end, through the extrusion of the edge-sealing airbag 103, the problem of cross-infection inside the reaction syringe 11 caused by the circulation of air is avoided. First, the side of the sealing film plate 1 is clamped along the first Slot 601 is slidingly installed. When the film sealing plate 1 is fastened to the skirt 12, press it down firmly. The film sealing plate 1 is made of pvc material and has the characteristics of elasticity, so that both sides move downward through the smooth bar 603, and pass through The round rods 603 are aligned to limit the position, replacing the traditional glue layer fixation, avoiding the problem of cross-infection caused by later loosening, and providing stable conditions for the culture of PCR plate cells.

In this embodiment, the shape of the reaction plate 6 is rectangular, and the skirt 12 is fixedly connected to the long sides and the wide side of the reaction plate 6, and the drainage groove 606 extends to the side of the reaction plate 6 where the skirt 12 is not provided. On the wide side, the outer wall of each reaction syringe slot 605 is provided with a round hole 608, each round hole 608 is opened on the upper surface of the reaction plate 6, and the inner wall of each round hole 608 is provided with a slope 607, and Each circular hole 608 is matched with the upper cover plate 104 of the reaction syringe. Firstly, each reaction syringe 11 is placed on the reaction syringe slot 605, and then the nutrient solution is poured into the reaction syringe 11. Then place the reaction plate 6 on the lower supporting plate 2, and fix it in a limited position. If there is a small volume and caliber inside the reaction cylinder when dripping the nutrient solution, it is easy to make the nutrient solution drop onto the plate before wiping. At this time, it will also cause cross-infection. At this time, the knob 506 can be turned to rotate through the cooperation of the turbine 502 and the worm 505. At the same time, the cooperation of the screw sleeve and the screw 501 will make the lower fixing rod 503 move downward. The ball 504 provided at the lower end can enable the reaction plate 6 to be stably supported when it deviates to one side.

In this embodiment, the upper cover plate 104 of the reaction syringe is fixedly connected to the lower surface of the film sealing plate 1, the outer wall of the upper cover plate 104 of the reaction syringe is fixedly connected to the edge sealing air bag 103, and the lower surface of the sealing plate 1 is fixed The upper cover plate 101 of the drainage groove is connected, and the end of the upper cover plate 101 of each drainage groove is fixedly connected with the sealing plate 102. The trimming assembly 5 includes a worm wheel 502, a lower fixed rod 503, a worm screw 505, and a knob 506. The knob 506 is fixedly connected to the One end of the worm 505, the worm 505 is rotatably connected to the inside of the lower pallet 2 and the end connected with the knob 506 is located outside the lower pallet 2, the worm 505 is meshed with the worm wheel 502, and the worm wheel 502 is arranged inside the lower pallet 2, which is easy When the nutrient solution drips onto the board, it will also cause cross-infection when wiping. At this time, the knob 506 can be turned to rotate through the cooperation of the turbine 502 and the worm 505. At the same time, the cooperation of the screw sleeve and the screw 501 can further fix the The rod 503 moves downward. During the movement, the ball 504 provided at the lower end can make the reaction plate 6 stable when it is shifted to one side, so that the nutrient solution dripping on the reaction plate 6 can pass through the drainage. The grooves 606 flow out, thereby avoiding the problem of cross-contamination caused by wiping.

In this embodiment, the inside of the worm wheel 502 is provided with a screw hole and the screw hole is threadedly matched with the screw rod 501. The upper and lower ends of the screw rod 501 are fixedly connected with the lower fixing rod 503, and the lower fixing rod 503 at the upper end is movably connected to the lower bracket. The inner wall of the plate 2 is fixedly connected with a ball 504 at the lower end of the lower fixed rod 503 at the lower end, and a horizontal groove 4 is provided on one side of the lower supporting plate 2, and a rolling ball 3 is arranged inside the horizontal groove 4, and the upper portion of the lower supporting plate 2 The surface is fixedly connected with an upper plate 9, and each upper plate 9 is provided with a storage inner hole 7, and the upper surface of the lower supporting plate 2 is provided with a limit hole 8, and the limit hole 8 cooperates with the locking limit rod 10, and the upper end is provided with The edge-sealing airbag 103, through the extrusion of the edge-sealing airbag 103, avoids the problem of cross-infection inside the reaction syringe 11 caused by the circulation of air. When the film sealing plate 1 is fastened to the skirt 12, press down hard, and the film sealing plate 1 adopts the characteristic of elasticity made of pvc material, so that both sides move downward through the smooth bar 603.

In this embodiment, the inner wall of the skirt 12 is provided with a U-shaped groove 602, and the middle of the U-shaped groove 602 is fixedly connected with a round rod 603. The lower end of the lower end is set as the second clamping groove 604, and the edge sealing air bag 103 is provided at the upper end. Through the extrusion of the edge sealing air bag 103, the problem of cross infection inside the reaction syringe 11 caused by the circulation of air is avoided. The side of the membrane plate 1 is slidably installed along the first clamping groove 601. When the membrane sealing plate 1 is clamped to the skirt 12, press it down firmly. The PVC material of the sealing membrane 1 has the characteristics of elasticity, making it The two sides move down through the round rod 603 .

The working principle of this cross-infection-proof PCR plate: first place each reaction syringe 11 on the reaction syringe slot 605, then pour the nutrient solution into the inside of the reaction syringe 11, and then put the reaction plate 6 Place it on the lower supporting plate 2 and fix it at the limit. If there is a small volume and caliber inside the reaction cylinder when the nutrient solution is dripped, it is easy to make the nutrient solution drop onto the plate. When wiping, it will also cause Cross-infection, at this time, you can turn the knob 506, through the cooperation of the turbine 502 and the worm 505, and at the same time, the cooperation of the screw sleeve and the screw 501, so that the lower fixing rod 503 moves downward. The ball 504 can make the reaction plate 6 stably support when it deviates to one side, so that the nutrient solution dripping on the reaction plate 6 can flow out through the drainage groove 606, thereby avoiding the problem of cross-infection caused by wiping.

At the same time, the edge-sealing airbag 103 provided on the upper end, through the extrusion of the edge-sealing airbag 103, the problem of cross-infection inside the reaction syringe 11 caused by the circulation of air is avoided. First, the side of the sealing film plate 1 is clamped along the first Slot 601 is slidingly installed. When the film sealing plate 1 is fastened to the skirt 12, press it down firmly. The film sealing plate 1 is made of pvc material and has the characteristics of elasticity, so that its two sides move downward through the smooth bar 603. The round rods 603 are aligned to limit the position, replacing the traditional glue layer fixation, avoiding the problem of cross-infection caused by later loosening, and providing stable conditions for the culture of PCR plate cells.

The basic principles and main features of the present invention and the advantages of the present invention have been shown and described above. Those skilled in the industry should understand that the present invention is not limited by the above-mentioned embodiments. What are described in the above-mentioned embodiments and the description only illustrate the principle of the present invention. Without departing from the spirit and scope of the present invention, the present invention will also have Variations and improvements are possible, which fall within the scope of the claimed invention. The protection scope of the present invention is defined by the appended claims and their equivalents.

Claims

A PCR plate for preventing cross-infection, including a reaction plate (6), characterized in that: the reaction plate (6) is fastened on the upper surface of the lower supporting plate (2), and the surface of the reaction plate (6) is set There are several reaction syringes (11), the upper surface of each reaction syringe (11) is sealed by the sealing film plate (1), and the surface of the reaction plate (6) is provided with reaction syringes (11 ) with the same number of reaction syringe slots (605), drainage slots (606) are arranged between the reaction syringe slots (605) in each row, and the drainage slots (606) are set on the reaction plate ( 6), the side wall of the reaction plate (6) is provided with a skirt (12), and the lower surface of the lower supporting plate (2) is symmetrically provided with four lower fixing rods (503) on the transverse centerline , and each of the lower fixing rods (503) slides for a short distance through the fine-tuning assembly (5), and the lower surface of the upper sealing film plate (1) is also fixedly connected with the same number of reaction syringes (11) The upper cover plate (101) of the drainage groove, and the upper cover plate (101) of the drainage groove is matched and clamped on the reaction syringe (11).
A cross-infection-proof PCR plate according to claim 1, characterized in that: the shape of the reaction plate (6) is rectangular, and the skirt (12) is fixedly connected to two sides of the reaction plate (6). On one side long side and one side wide side, the drainage groove (606) extends to one side wide side of the reaction plate (6).
A cross-infection-proof PCR plate according to claim 1, characterized in that: each of the reaction syringe slots (605) is provided with a round hole (608) on the outer wall, and each of the round holes ( 608) are provided on the upper surface of the reaction plate (6).
A cross-infection-proof PCR plate according to claim 1, characterized in that: the inner wall of each of the circular holes (608) is provided with a slope (607), and each of the circular holes (608) cooperates Tighten the top cover (104) with the reaction syringe.
A cross-infection-proof PCR plate according to claim 4, characterized in that: the upper cover plate (104) of the reaction syringe is fixedly connected to the lower surface of the sealing film plate (1), and the upper cover plate (104) of the reaction syringe An edge-banding airbag (103) is fixedly connected to the outer wall of the cover plate (104).
A cross-infection-proof PCR plate according to claim 5, characterized in that: and the lower surface of the sealing plate (1) is fixedly connected with an upper cover plate (101) of a drainage groove, and each of the drainage grooves A sealing plate (102) is fixedly connected to the end of the upper cover plate (101).
A cross-infection-proof PCR plate according to claim 1, characterized in that: the fine-tuning assembly (5) includes a worm gear (502), a lower fixing rod (503), a worm (505), and a knob (506), The knob (506) is fixedly connected to one end of the worm (505), and the worm (505) is rotatably connected to the inside of the lower pallet (2) and the end connected to the knob (506) is located at the bottom of the lower pallet (2). Externally, the worm (505) is mated with the worm wheel (502), and the worm wheel (502) is arranged inside the lower supporting plate (2).
A PCR plate for preventing cross-infection according to claim 7, characterized in that: the inside of the worm wheel (502) is provided with a screw hole and the screw hole is threadedly matched with the screw rod (501), and the screw rod (501) ) are fixedly connected with a lower fixing rod (503) at the upper and lower ends, the lower fixing rod (503) at the upper end is movably connected to the inner wall of the lower supporting plate (2), and the lower fixing rod (503) at the lower end is fixed A ball (504) is attached.
A cross-infection-proof PCR plate according to claim 1, characterized in that: a horizontal groove (4) is opened on one side of the lower supporting plate (2), and a horizontal groove (4) is arranged inside the horizontal groove (4) Rolling balls (3), the upper surface of the lower supporting plate (2) is fixedly connected with an upper plate (9), each of the upper plates (9) is provided with a storage inner hole (7), and the lower supporting plate ( 2) A limiting hole (8) is provided on the upper surface, and the limiting hole (8) cooperates with the clamping limiting rod (10).
A cross-infection-proof PCR plate according to claim 1, characterized in that: the inner wall of the skirt (12) is provided with a U-shaped groove (602), and the middle of the U-shaped groove (602) is fixedly connected There is a round rod (603), the upper end of the round rod (603) is set as the first clamping groove (601), and the lower end of the round rod (603) is set as the second clamping groove (604).