CN217025978U

CN217025978U - Mesenchymal stem cell perfusion culture bioreactor

Info

Publication number: CN217025978U
Application number: CN202220394281.3U
Authority: CN
Inventors: 董向涛; 刘玮
Original assignee: Hebei Sanzang Biotechnology Co ltd
Current assignee: Hebei Sanzang Biotechnology Co ltd
Priority date: 2022-02-25
Filing date: 2022-02-25
Publication date: 2022-07-22
Anticipated expiration: 2032-02-25

Abstract

The utility model discloses a mesenchymal stem cell perfusion culture bioreactor which comprises a connecting seat, a mounting column, a bottle cap, a perfusion chamber, a through hole block group, a screw rod, a silk block, a rotating block, a peristaltic pump, a connecting pipe and a conveying pipe, wherein a placing groove is formed in one side above the connecting seat, a mounting groove is formed in one end of the mounting column, a through pipe group is fixedly inserted in the bottle cap in a penetrating and embedding way, a liquid storage bottle is mounted above the connecting seat through a limiting column, the upper part of the perfusion chamber is fixedly embedded with one end of a flow guide pipe, the perfusion chamber is mounted on one side of the mounting column through the through hole mounting seat group, the through hole block group is fixedly provided with the connecting bearing, and the through hole block group is fixedly connected with the mounting column through the mounting groove, and the through hole mounting seat group is used for achieving the effect of fixed connection.

Description

Mesenchymal stem cell perfusion culture bioreactor

Technical Field

The utility model relates to the technical field of mesenchymal stem cells, in particular to a perfusion culture bioreactor for mesenchymal stem cells.

Background

The mesenchymal stem cell is a multipotent stem cell, has all commonalities of stem cells, namely self-renewal and multidirectional differentiation capacity, is most clinically applied, is combined with hematopoietic stem cells, can improve the success rate of transplantation and accelerate hematopoietic reconstruction, can obviously accelerate the recovery time of blood cells of a patient by inputting the mesenchymal stem cells and the hematopoietic stem cells together after the patient receives a large dose of chemotherapy, is safe and has no adverse reaction, a bioreactor is usually required for large-scale cell culture in the production of biological products, most of the traditional bioreactor culture is fed batch culture, but in recent years, the interest in continuous processes is continuously increased, and compared with batch or fed batch culture, perfusion culture can provide better nutrition and environment for the growth of the cells, so that the cells can reach a better state, and finally, higher cell density and correspondingly higher unit protein yield can be achieved, what brings like this is that, perfusion culture can obtain the target product of an order of magnitude more, and present mesenchymal stem cell perfusion culture bioreactor all is large-scale cultivation equipment to lead to the bioengineering teaching in-process can't make the student carry out the condition of manual operation to large-scale mesenchymal stem cell perfusion culture bioreactor, not only can't improve the teaching quality like this and still lead to bioengineering student can't experience the condition of in fact hand operation.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a mesenchymal stem cell perfusion culture bioreactor aiming at the defects of the prior art, and aims to solve the problems that the conventional mesenchymal stem cell perfusion culture bioreactor in the background technology is large-scale culture equipment, so that students cannot manually operate the large-scale mesenchymal stem cell perfusion culture bioreactor in the bioengineering teaching process, the teaching quality cannot be improved, and the bioengineering students cannot experience actual manual operation.

In order to achieve the purpose, the utility model provides the following technical scheme: a mesenchymal stem cell perfusion culture bioreactor comprises a connecting seat, a mounting column, a bottle cap, a perfusion chamber, a through hole block group, a screw rod, a silk block, a rotating block, a peristaltic pump, a connecting pipe and a conveying pipe, wherein a placing groove is arranged on one side above the connecting seat, a limiting column is fixed on the other side above the connecting seat, an installation groove is arranged at one end of the installation column, a through hole installation seat group is fixed on one side of the installation column, meanwhile, the other end of the mounting column is fixedly connected with one side above the connecting seat, the bottle cap is fixedly embedded with a through pipe group in a penetrating way, the bottle cap is connected with the liquid storage bottle through threads, the liquid storage bottle is arranged above the connecting seat through a limiting column, the upper part of the perfusion chamber is fixedly embedded with one end of the flow guide pipe, the perfusion chamber is arranged on one side of the mounting column through the through hole mounting seat group, the through hole block group is fixedly provided with a connecting bearing, and the through hole block group is fixedly connected with the mounting column through the mounting groove;

by adopting the technical scheme, the through hole mounting seat group and the connecting bearing are arranged, the through hole mounting seat group not only has the effect of matching and fixedly connecting with the connecting bearing, but also has the effect of fixedly connecting through the through hole mounting seat group, and simultaneously, the connecting bearing has the effect of enabling the inner ring to penetrate through the matching and fixedly connecting, the connecting bearing is sequentially connected to have the effect of forced driving and forced relative rotation movement, the connecting rod group is supported and connected through the connecting bearing in a vertical stressed manner, and the condition that the integral connecting rod group cannot be matched and vertically installed due to the fact that no stressed vertical supporting point exists when the connecting rod group is installed in the mounting column is avoided;

the lead screw both ends are fixed with connecting rod group, silk piece one side is fixed with another through-hole mount pad group through the connecting plate, the lead screw runs through a piece and a through-hole block group through connecting rod group and is connected with the connection bearing rotation, rotatory piece and connecting rod group fixed connection, the peristaltic pump passes through the standing groove and installs in the connecting seat top, connecting pipe one end is run through first sealing washer and is connected with siphunculus matched stack, and the connecting pipe other end is connected with the peristaltic pump, the conveyer pipe both sides are run through second sealing washer group and are connected with another siphunculus group and honeycomb duct other end supporting respectively.

Through adopting above-mentioned technical scheme, be equipped with rotatory piece, play fixed connection's effect equally through rotatory piece one side, and play the effect that the atress drove and atress syntropy rotary motion equally through rotatory piece, thereby play the effect that makes things convenient for the fast rotation to drive above the connecting rod group through rotatory piece simultaneously, thereby avoid connecting rod group to be slick and sly state in proper order, thereby can't apply force and drive the circumstances that connecting rod group carries out the atress rotation when leading to operator's hand to be stained with liquid or hand perspire.

Preferably, the distance of the transverse diameter of the limiting column is more than 2-4cm than the distance of the transverse diameter of the bottom of the liquid storage bottle.

Through adopting the above-mentioned technical scheme, be equipped with spacing post, play fixed connection's effect through spacing post one end, and play the effect that the atress drove equally through spacing post, spacing post is provided with 2 groups 4 simultaneously, 4 spacing post connection shapes are the square in proper order, it not only plays the spacing effect of motion to stock solution bottle bottom to be the square through spacing post connection shape, thereby avoid spacing post to be provided with 1 group 2, the spacing post that not only leads to setting up can't play the spacing effect of motion like this, still cause the spacing post that sets up to become the useless condition that sets up the part unnecessarily.

Preferably, the screw rod, the connecting rod group, the screw block, the other through hole mounting seat group and the rotating block form a rotary lifting mechanism, and the rotary lifting distance of the rotary lifting mechanism ranges from 0cm to 10 cm.

Through adopting above-mentioned technical scheme, be equipped with another through-hole mount pad group, another through-hole mount pad group not only plays fixed connection's effect, and play the atress through another through-hole mount pad group equally and drive, the effect of atress syntropy motion and atress relative motion, another through-hole mount pad group through-hole lateral diameter ratio bottle lid lateral diameter is too big simultaneously, make things convenient for smoothly to run through the bottle lid in another through-hole mount pad group atress motion process in proper order and carry out spacing contact with the stock solution bottle, play the spacing effect of motion to the stock solution bottle top through another through-hole mount pad group once more, avoid through-hole mount pad group only to carry out the condition that spacing contact leads to the stock solution bottle top to be in the atress motion state with the stock solution bottle bottom, lead to the condition that the stock solution bottle drops through-hole mount pad group top easily in the connecting seat atress motion process in proper order.

Preferably, the connection shape of the wire block and the other through hole mounting seat group is horizontally placed in a T shape.

Through adopting above-mentioned technical scheme, be equipped with the silk piece, not only play one side fixed connection's effect through the silk piece to thereby play through connection's effect through the silk piece, thereby play the effect that atress drove and atress relative motion through the silk piece equally simultaneously, thereby loop through the silk piece and play the effect of motion locking to another through-hole mount pad group.

Preferably, the distance range of the connecting gap between the first sealing ring and the through pipe group is 0.2-0.35 mm.

Through adopting above-mentioned technical scheme, be equipped with first sealing washer, play through connection's effect equally through first sealing washer to play the effect that the atress drove and atress relative motion equally through first sealing washer, thereby play sealing connection's effect to the siphunculus group through first sealing washer simultaneously.

Preferably, the whole shape of the conveying pipe is L-shaped, and the vertical length interval range of one end of the conveying pipe is 8-15 cm.

Through adopting above-mentioned technical scheme, be equipped with the conveyer pipe, not only play the effect of carrying the connection through the conveyer pipe to the through connection gap distance between conveyer pipe and the second sealing washer is 0.2mm, thereby avoids having great through connection gap between conveyer pipe and the second sealing washer, influences the sealing connection nature between the two above-mentioned like this.

Compared with the prior art, the utility model has the beneficial effects that: the mesenchymal stem cell perfusion culture bioreactor,

(1) the device is provided with a connecting seat and an installation column, the whole device is easily moved to a corresponding placing position by manually carrying the connecting seat and the installation column to move, when the connecting seat and the installation column are moved to the corresponding installation positions, other devices and the connecting seat are installed in a matched mode again, and meanwhile, the problems that the existing mesenchymal stem cell perfusion culture bioreactor is large-scale culture equipment, so that a student cannot manually operate the large-scale mesenchymal stem cell perfusion culture bioreactor in the bioengineering teaching process, the teaching quality cannot be improved, and the bioengineering student cannot experience actual manual operation are sequentially avoided through the connecting seat and the installation column;

(2) the device is provided with a bottle cap, a liquid storage bottle and a perfusion chamber, an operator manually connects the bottle cap and the liquid storage bottle in a rotating mode, then the operator manually places and installs the liquid storage bottle above the connecting seat, and meanwhile manually places the perfusion chamber above the through hole installation seat group, so that the student can be gradually familiar with the installation and operation steps in the manual operation process of the operator or the student, and the situations are avoided again;

(3) be provided with rotatory piece, first sealing washer and second sealing washer, the operator passes through the motion of the rotatory piece of manual regulation, and rotatory piece atress motion in-process drives connecting rod group syntropy motion, thereby connect the rotatory in-process of group atress through the lead screw drive another through-hole mount pad group motion and carry out spacing connection to the perfusion room top simultaneously, thereby the operator is familiar with the step of perfusion room quick installation and separation through the rotatory piece motion in-process of manual regulation, thereby the operator makes connecting pipe and conveyer pipe carry out the effect of quick installation and separation through the first sealing washer of manual drive and the second sealing washer motion in-process, avoid foretell condition once more through the operator in person's operation process equally.

Drawings

FIG. 1 is a schematic front sectional view of the present invention;

FIG. 2 is an enlarged view of the structure at A in FIG. 1 according to the present invention;

FIG. 3 is an enlarged view of the structure at B in FIG. 1 according to the present invention;

FIG. 4 is an enlarged view of the structure of FIG. 1 at C;

fig. 5 is a schematic structural view of the through pipe group, the connecting pipe and the first sealing ring of the utility model.

In the figure: 1. the device comprises a connecting seat, 2 parts of a limiting column, 3 parts of an installation column, 4 parts of a through hole installation seat group, 5 parts of a bottle cap, 6 parts of a through pipe group, 7 parts of a liquid storage bottle, 8 parts of a perfusion chamber, 9 parts of a flow guide pipe, 10 parts of a through hole block group block, 11 parts of a lead screw, 12 parts of a connecting rod group, 13 parts of a silk block, 14 parts of a rotating block, 15 parts of a peristaltic pump, 16 parts of a connecting pipe, 17 parts of a first sealing ring, 18 parts of a conveying pipe, 19 parts of a second sealing ring group.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-5, the present invention provides a technical solution: a mesenchymal stem cell perfusion culture bioreactor, as shown in figure 1, one side above a connecting seat 1 is provided with a placing groove, the other side above the connecting seat 1 is fixed with a limiting column 2, the transverse diameter distance of the limiting column 2 is more than 2-4cm larger than the transverse diameter distance of the bottom of a liquid storage bottle 7, if the transverse diameter distance of the limiting column 2 is too small than the transverse diameter distance of the bottom of the liquid storage bottle 7, an operator manually installs the liquid storage bottle 7 above the connecting seat 1 through the limiting column 2, the liquid storage bottle 7 can not be installed above the connecting seat 1 in a matching way through the limiting column 2 due to the fact that the transverse diameter distance of the bottom of the liquid storage bottle 7 is too large than the transverse diameter distance of the limiting column 2, and when the limiting column 2 can not be installed in a matching way, the limiting column 2 needs to be installed fixedly again or the whole liquid storage bottle 7 needs to be installed in a matching way, therefore, the situation is avoided, and the distance between the transverse diameters of the limiting columns 2 is larger than the distance between the transverse diameters of the bottoms of the liquid storage bottles 7 by 2 cm.

As shown in FIG. 2, one end of the mounting post 3 is provided with a mounting groove, one side of the mounting post 3 is fixed with a through hole mounting seat group 4, while the other end of the mounting post 3 is fixedly connected with one side above the connecting seat 1, the bottle cap 5 is fixedly inserted with a through pipe group 6, the bottle cap 5 is in threaded connection with a liquid storage bottle 7 through threads, the liquid storage bottle 7 is arranged above the connecting seat 1 through a limiting post 2, the upper part of a perfusion chamber 8 is fixedly inserted with one end of a flow guide pipe 9, the perfusion chamber 8 is arranged at one side of the mounting post 3 through the through hole mounting seat group 4, a connecting bearing is fixed on the through hole block group 10, the through hole block group 10 is fixedly connected with the mounting post 3 through the mounting groove, two ends of a screw rod 11 are fixed with a connecting rod group 12, one side of a thread 13 is fixed with another through hole mounting seat group 4 through a connecting plate, the thread 13 is connected with another through hole mounting seat group 4 in a horizontally placed T shape, the two are horizontally placed T-shaped through the connecting shape, the connection simplicity between the two is embodied, the practicability and the stress drive of the connection between the two are embodied, the isotropy and the synchronous motion of the two are embodied again, and meanwhile, the connection shape between the two is horizontally placed in a T shape, so that the symmetry between the two is embodied in sequence.

As shown in fig. 4, the screw rod 11 is connected with the connecting bearing by the connecting rod group 12 through the screw block 13 and the through hole block group 10, the rotating block 14 is fixedly connected with the connecting rod group 12, the screw rod 11, the connecting rod group 12, the screw block 13, the other through hole mounting base group 4 and the rotating block 14 form a rotary lifting mechanism, and the rotary lifting mechanism has a rotary lifting distance range of 0-10cm, when the other through hole mounting seat group 10 needs to be adjusted, the operator manually adjusts to drive the rotating block 14 to move, and the rotating block 14 drives the screw rod 11 to move in the same direction through the connecting rod group 12 in the process of bearing force, meanwhile, in the forced rotation process of the screw rod 11, the other through hole mounting seat group 4 is driven by the screw block 13 to move relative to the through hole block group block 10 and the mounting column 3, and the rotating and lifting distance interval of the integral rotating and lifting mechanism is 10cm, and the rotating and lifting distance interval of the integral rotating and lifting mechanism is the vertical movement distance interval of the wire block 13.

As shown in fig. 3 and 5, the peristaltic pump 15 is installed above the connection seat 1 through the placement groove, one end of the connection pipe 16 penetrates through the first sealing ring 17 and is connected with the through pipe set 6 in a matching manner, the distance of the connection gap between the first sealing ring 17 and the through pipe set 6 ranges from 0.2mm to 0.35mm, if the distance of the connection gap between the first sealing ring 17 and the through pipe set 6 is too large, the first sealing ring 17 sinks inside the through pipe set 6 under the influence of gravity, when the first sealing ring 17 sinks inside the through pipe set 6, the first sealing ring 17 cannot achieve the matching connection effect, and also causes the two to have a large connection gap, and the connection tightness between the two is reduced in sequence, so that the above situation is avoided and the distance of the connection gap between the first sealing ring 17 and the through pipe set 6 is set to be 0.25mm, the other end of the connecting pipe 16 is connected with the peristaltic pump 15, the two sides of the conveying pipe 18 penetrate through the second sealing ring group 19 to be respectively matched and connected with the other end of the other through pipe group 6 and the other end of the draft tube 9, the whole shape of the conveying pipe 18 is L-shaped, the vertical length interval range of one end of the conveying pipe 18 is 8-15cm, through the L-shaped overall shape of the structural member, the through connectivity and the conveying connectivity effects of the structural member are embodied, and the connecting shape of the structural parts is L-shaped again, meanwhile, the effect of fast penetrating and matching connection between the two ends of the conveying pipe 18 and the other through pipe group 6 and the guide pipe 9 is facilitated, the vertical length of one end of the conveying pipe 18 is 15cm away from the equipment in sequence, so not only make things convenient for conveyer pipe 18 to run through siphunculus group 6 and extend and carry out the perfusion in stock solution bottle 7 is inside, thereby still avoid 18 one end vertical distances of conveyer pipe to run through the condition of extension in short-term excessively.

The working principle is as follows: in the perfusion culture bioreactor using the mesenchymal stem cells, firstly, a connecting seat 1 and a mounting column 3 are manually carried at corresponding positions, then the mesenchymal stem cells are conveyed into a perfusion chamber 8 through a guide pipe 9, the perfusion chamber 8 is placed above a through hole mounting seat group 4 after the filling of the mesenchymal stem cells is finished, then, a rotating block 14 is manually adjusted to move, in the stress process of the rotating block 14, a screw rod 11 is driven to move through a connecting rod group 12, simultaneously, in the stress process of the screw rod 11, another through hole mounting seat group 4 and a through hole block group 10 are driven to move relatively through a screw block 13, when another through hole mounting seat group 4 is contacted with the upper part of the perfusion chamber 8, the rotating block 14 is stopped to move, then, culture solution is filled into a liquid storage bottle 7, after the filling of the culture solution is finished, the liquid storage bottle 7 is connected with the liquid storage bottle 7 through a bottle cap 5, and then, the liquid storage bottle 7 is mounted above the connecting seat 1 through a limiting column 2, meanwhile, the peristaltic pump 15 is installed above the connection seat 1 again, the connection pipe 16 and the peristaltic pump 15 are connected again manually, the connection pipe 16 is connected with the tube group 6 through the first sealing ring 17 again, and the conveying pipe 18 is simultaneously connected with the flow guide pipe 9 and the other tube group 6 through the second sealing ring group 19 respectively, after the above devices are connected, the peristaltic pump 15 is connected with the socket, and the culture solution is conveyed into the perfusion chamber 8 through the conveying pipe 18 to perform perfusion culture through the working process of the peristaltic pump 15, so that the whole operation is completed, and the content which is not described in detail in the specification belongs to the prior art known to the skilled person in the art.

The terms "central," "longitudinal," "lateral," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in the orientation or positional relationship indicated in the drawings for simplicity of description only and are not intended to indicate or imply that the referenced devices or elements must be in a particular orientation, constructed and operative in a particular orientation, and are not to be considered limiting of the claimed invention.

Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described in the foregoing embodiments, or equivalents may be substituted for elements thereof.

Claims

1. The utility model provides a bioreactor is cultivateed in mesenchymal stem cell perfusion, includes connecting seat (1), erection column (3), bottle lid (5), perfusion chamber (8), through-hole block group (10), lead screw (11), silk piece (13), rotatory piece (14), peristaltic pump (15), connecting pipe (16) and conveyer pipe (18), its characterized in that:

a placing groove is arranged on one side above the connecting seat (1), a limiting column (2) is fixed on the other side above the connecting seat (1), one end of the mounting column (3) is provided with a mounting groove, a through hole mounting seat group (4) is fixed at one side of the mounting column (3), meanwhile, the other end of the mounting column (3) is fixedly connected with one side above the connecting seat (1), a through pipe group (6) is fixedly embedded in the bottle cap (5) in a penetrating way, the bottle cap (5) is connected with the liquid storage bottle (7) through threads, the liquid storage bottle (7) is arranged above the connecting seat (1) through the limiting column (2), the upper part of the perfusion chamber (8) is embedded and fixed with one end of the draft tube (9), and the perfusion chamber (8) is arranged at one side of the mounting column (3) through a through hole mounting seat group (4), a connecting bearing is fixed on the through hole block group (10), the through hole block group (10) is fixedly connected with the mounting column (3) through the mounting groove;

lead screw (11) both ends are fixed with connecting rod group (12), silk piece (13) one side is fixed with another through-hole mount pad group (4) through the connecting plate, lead screw (11) run through silk piece (13) and through-hole block group (10) through connecting rod group (12) and are connected with the connecting bearing rotation, rotatory piece (14) and connecting rod group (12) fixed connection, peristaltic pump (15) are installed in connecting seat (1) top through the standing groove, connecting pipe (16) one end is run through first sealing washer (17) and is organized (6) supporting connection with the siphunculus, and the connecting pipe (16) other end is connected with peristaltic pump (15), conveyer pipe (18) both sides are run through second sealing washer group (19) and are organized (6) and honeycomb duct (9) other end supporting connection with another siphunculus respectively.

2. A mesenchymal stem cell perfusion culture bioreactor according to claim 1, wherein: the transverse diameter distance of the limiting column (2) is 2-4cm larger than the transverse diameter distance of the bottom of the liquid storage bottle (7).

3. A mesenchymal stem cell perfusion culture bioreactor according to claim 1, wherein: the screw rod (11), the connecting rod group (12), the screw block (13), the other through hole mounting seat group (4) and the rotating block (14) form a rotary lifting mechanism, and the rotary lifting mechanism rotates to lift within a distance range of 0-10 cm.

4. A mesenchymal stem cell perfusion culture bioreactor according to claim 1, wherein: the connection shape of the thread block (13) and the other through hole mounting seat group (4) is horizontally arranged in a T shape.

5. A mesenchymal stem cell perfusion culture bioreactor according to claim 1, wherein: the distance range of a connecting gap between the first sealing ring (17) and the through pipe group (6) is 0.2-0.35 mm.

6. A mesenchymal stem cell perfusion culture bioreactor according to claim 1, wherein: the conveying pipe (18) is L-shaped in overall shape, and the distance range of the vertical length of one end of the conveying pipe (18) is 8-15 cm.