CN206428253U - The union component of cellular-type eight - Google Patents
The union component of cellular-type eight Download PDFInfo
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- CN206428253U CN206428253U CN201720036975.9U CN201720036975U CN206428253U CN 206428253 U CN206428253 U CN 206428253U CN 201720036975 U CN201720036975 U CN 201720036975U CN 206428253 U CN206428253 U CN 206428253U
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- reaction tube
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Abstract
The utility model discloses a kind of union component of cellular-type eight, it includes eight reaction tubes and seven reaction pipe connections, eight reaction tubes are linearly arranged, it is connected between two neighboring reaction tube by a reaction pipe connections, reaction tube includes reaction tube main body, conical bottom pipe, dividing plate connecting pole, preceding dividing plate, right clapboard, rear bulkhead, left clapboard, the first reative cell, the second reative cell, the 3rd reative cell, the 4th reative cell, embedded lid.Each reaction tube of the union component of cellular-type eight is separated processing by the utility model, each reaction tube of eight unions after processing is owned by four independent reative cells, the quantity of performing PCR or RT PCR reactions is entered in increase simultaneously, improve PCR or RT PCR conventional efficient, more large batch of amplified reaction is realized, the service efficiency of PCR instrument is greatly improved.
Description
Technical field
The utility model is related to a kind of eight unions component, more particularly to a kind of union component of cellular-type eight.
Background technology
PCR（Polymerase Chain Reaction, polymerase chain amplification reaction）Instrument is to utilize DNA（Deoxyribose
Nucleic acid）Polymerase makees the molecular diagnostic assays instrument largely synthesized in external or test tube to specific gene, and PCR instrument is generally used for
PCR or RT-PCR reactions, both experiments are denatured DNA using heating, make in the presence of polymerase it is single-stranded be copied into double-strand,
And then reach the purpose of gene duplication.PCR instrument needs to consume substantial amounts of experiment reaction tube, traditional experiment reaction tube knot in experiment
Structure is relatively common, and the reagent volumes of reaction tube have been overflowed relative to the PCR experiment of single, causes the reaction reagent wave of experiment
Take, experimental cost is expensive.
The content of the invention
Technical problem to be solved in the utility model is to provide a kind of union component of cellular-type eight, and it is by cellular-type eight
Each reaction tube of tube assembly is separated processing, and each reaction tube of eight unions after processing is owned by four independent reactions
The quantity of performing PCR or RT-PCR reactions is entered in room, increase simultaneously, improves PCR or RT-PCR conventional efficient, realizes more large batch of
Amplified reaction, greatly improves the service efficiency of PCR instrument, and the amplification number of times of PCR instrument can be reduced to a certain extent, so as to
Extend the service life of PCR instrument, the segregated portion of embedded lid be able to can be loaded in the case of covered, be loaded and from
After the heart, the component of addition enters corresponding pipe shaft marker space, reduces the carry out difficulty of the reaction of secondary amplification, it is to avoid draw because uncapping
The pollution risen.
The utility model is to solve above-mentioned technical problem by following technical proposals：A kind of union group of cellular-type eight
Part, it is characterised in that it includes eight reaction tubes and seven reaction pipe connections, and eight reaction tubes are linearly arranged, adjacent
It is connected between two reaction tubes by a reaction pipe connections, reaction tube includes reaction tube main body, conical bottom pipe, dividing plate connection
It is post, preceding dividing plate, right clapboard, rear bulkhead, left clapboard, the first reative cell, the second reative cell, the 3rd reative cell, the 4th reative cell, embedding
Enter formula lid, reaction tube main body is fixedly connected with conical bottom pipe, dividing plate connecting pole is located at reaction tube main body and conical bottom tube hub
And dividing plate connecting pole bottom is fixedly connected with conical bottom bottom of the tube, preceding dividing plate one end is connected with dividing plate connecting pole, and preceding dividing plate is another
End is connected with reaction tube main body and conical bottom pipe respectively, and right clapboard one end is connected with dividing plate connecting pole, right clapboard other end difference
Be connected with reaction tube main body and conical bottom pipe, rear bulkhead one end is connected with dividing plate connecting pole, the rear bulkhead other end respectively with reaction
Pipe main body is connected with conical bottom pipe, and left clapboard one end is connected with dividing plate connecting pole, the left clapboard other end respectively with reaction tube main body
It is connected with conical bottom pipe, preceding dividing plate, right clapboard, rear bulkhead, the left clapboard angle spaced apart ninety degrees from successively centered on dividing plate connecting pole
Arrangement, preceding dividing plate, right clapboard, reaction tube main body, conical bottom pipe are interconnected to form the first reative cell, right clapboard, rear bulkhead, anti-
Should pipe main body, conical bottom pipe be interconnected to form the second reative cell, rear bulkhead, left clapboard, reaction tube main body, conical bottom pipe are mutual
Connection forms the 3rd reative cell, and left clapboard, preceding dividing plate, reaction tube main body, conical bottom pipe are interconnected to form the 4th reative cell, embedding
Enter formula lid positioned at the top of preceding dividing plate and embedded lid has cross separation structure.
Preferably, the embedded lid is provided with the section of a closure projection, reaction tube main body and conical bottom pipe
It is circle.
Preferably, the embedded lid provided with a sealing ring and sealing ring be located at the mouth of pipe of reaction tube main body with it is embedding
Enter at formula lid mating connection.
Preferably, multiple connecting pole pod apertures are provided with inside the embedded lid.
Preferably, seal recessed when the embedded lid closes embedded lid provided with a sealed groove and reaction tube
Groove is connected with preceding dividing plate, right clapboard, rear bulkhead, left clapboard respectively.
Positive effect of the present utility model is：The utility model is by each reaction tube of the union component of cellular-type eight
Processing is separated, each reaction tube of eight unions after processing is owned by four independent reative cells, and increase enters performing PCR simultaneously
（Polymerase Chain Reaction, polymerase chain amplification reaction）Or RT-PCR（Reverse Transcription-
Polymerase Chain Reaction, the reaction of reverse transcription polymerase chain amplification）The quantity of reaction, improves PCR or RT-PCR
Conventional efficient, realize more large batch of amplified reaction, greatly improve the service efficiency of PCR instrument, can subtract to a certain extent
The amplification number of times of few PCR instrument, so as to extend the service life of PCR instrument, the segregated portion of embedded lid can not opened
It can be loaded in the case of lid, after being loaded and centrifuging, the component of addition enters corresponding pipe shaft marker space, reduces secondary amplification
The carry out difficulty of reaction, it is to avoid polluted caused by uncapping.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model.
Fig. 2 is reaction tube structural representation of the present utility model.
Embodiment
The utility model preferred embodiment is provided below in conjunction with the accompanying drawings, to describe the technical solution of the utility model in detail.
As depicted in figs. 1 and 2, the union component of the utility model cellular-type eight includes eight reaction tubes 100 and seven reactions
Pipe connections 200, eight reaction tubes 100 are linearly arranged, and are connected between two neighboring reaction tube 100 by a reaction tube
Part 200 be connected, reaction tube 100 include reaction tube main body 1, conical bottom pipe 2, dividing plate connecting pole 3, preceding dividing plate 4, right clapboard 5, after every
Plate 6, left clapboard 7, the first reative cell 8, the second reative cell 9, the 3rd reative cell 10, the 4th reative cell 11, embedded lid 12, instead
Should pipe main body 1 be fixedly connected with conical bottom pipe 2, dividing plate connecting pole 3 be located at reaction tube main body 1 and the center of conical bottom pipe 2 and dividing plate
The bottom of connecting pole 3 is fixedly connected with the bottom of conical bottom pipe 2, and preceding one end of dividing plate 4 is connected with dividing plate connecting pole 3, the preceding other end of dividing plate 4
It is connected respectively with reaction tube main body 1 and conical bottom pipe 2, the one end of right clapboard 5 is connected with dividing plate connecting pole 3, the other end point of right clapboard 5
It is not connected with reaction tube main body 1 and conical bottom pipe 2, the one end of rear bulkhead 6 is connected with dividing plate connecting pole 3, the other end of rear bulkhead 6 difference
Be connected with reaction tube main body 1 and conical bottom pipe 2, the one end of left clapboard 7 is connected with dividing plate connecting pole 3, the other end of left clapboard 7 respectively with
Reaction tube main body 1 is connected with conical bottom pipe 2, and preceding dividing plate 4, right clapboard 5, rear bulkhead 6, left clapboard 7 are centered on dividing plate connecting pole 3
Angle spaced apart ninety degrees from arrangement successively, preceding dividing plate 4, right clapboard 5, reaction tube main body 1, that conical bottom pipe 2 is interconnected to form first is anti-
Room 8 is answered, right clapboard 5, rear bulkhead 6, reaction tube main body 1, conical bottom pipe 2 are interconnected to form the second reative cell 9, rear bulkhead 6, a left side
Dividing plate 7, reaction tube main body 1, conical bottom pipe 2 are interconnected to form the 3rd reative cell 10, left clapboard 7, preceding dividing plate 4, reaction tube master
Body 1, conical bottom pipe 2 are interconnected to form the 4th reative cell 11, and embedded lid 12 is located at the top of preceding dividing plate 4 and embedded pipe
Lid has cross separation structure.
Embedded lid 12 is provided with closure projection, and the section of reaction tube main body 1 and conical bottom pipe 2 is circle
Shape, reaction tube 100 is placed in PCR instrument, and closure ledge is used to contact with sealed membrane, realizes sealing.
The embedded inside of lid 12 is provided with multiple connecting pole pod apertures, when PCR is loaded, liquid can be added in into PCR pipe close
Capping 4 separates in position, and centrifugation, reaction solution enters reative cell through pod apertures.
Sealed groove is distinguished when embedded lid 12 closes embedded lid 12 provided with a sealed groove and reaction tube
It is connected with preceding dividing plate 4, right clapboard 5, rear bulkhead 6, left clapboard 7, sealed groove improves embedded lid 12 respectively to
One reative cell 8, the second reative cell 9, the 3rd reative cell 10, the sealing intensity of the 4th reative cell 11, are strengthened each inside reaction tube
The sealing of reative cell.
Sealing ring and sealed groove are that polypropylene material is made, and polypropylene material is not sent out with the reaction reagent of PCR experiment
Raw reaction, it is to avoid sealing ring and sealed groove and experiment reagent react influence experimental result.
Operation principle of the present utility model is as follows：The union component of the utility model cellular-type eight is used as consumptive material in experiment
In increase cross demarcation strip, reaction tube lid in diagnostic field, each reaction tube conical bottom pipe of the union component of cellular-type eight
In the cross demarcation strip of insertion reaction pipe.In a particular embodiment, the union component of cellular-type eight is PP（Polypropylene）Material system
Into the specification of single reaction tube is 0.2ml, and demarcation strip end is apart from the mouth of pipe about 5mm.Compared to the common response of only one of which reative cell
Pipe, each reaction tube of the union component of the utility model cellular-type eight is separated into four independent reative cells, added limited
The reaction quantity in space；Consider to repeat sample-adding convenience, common eight unions lid is improved, common eight unions cap upper surface is
It is divided into cylindricality inside plane, pipe, the utility model designs upper surface indent, single reaction tube accordingly does four separations, adds pipe
The liquid of lid is through centrifugation, you can flow into respective reaction room, realizes that noncontact is loaded, is conducive to continuous sample-adding, and play anti-intersection
Pollution effects.When entering performing PCR or RT-PCR reactions, a reaction tube can carry out four reactions simultaneously, can make 96 hole
PCR instrument can carry out 384 reactions simultaneously, so as to increase the PCR or RT-PCR reaction quantity under same Amplification, improve real
Test the purpose of efficiency.The segregated portion of supporting embedded lid can be in covered feelings with the union component pipe shaft of cellular-type eight
Sample-adding is realized under condition, in use, secondary amplification if needed, aluminium foil sealing can be used when expanding first time, expand for the first time
After end, aluminium foil is removed, is loaded in each marker space of embedded lid, after being loaded and centrifuging, the component of addition enters
Corresponding pipe shaft marker space, favorably needs to carry out the reaction of secondary amplification, it is to avoid polluted caused by uncapping.
In summary, each reaction tube of the union component of cellular-type eight is separated processing by the utility model, after processing
Each reaction tubes of eight unions be owned by four independent reative cells, the quantity of performing PCR or RT-PCR reactions is entered in increase simultaneously,
PCR or RT-PCR conventional efficient is improved, more large batch of amplified reaction is realized, greatly improves the service efficiency of PCR instrument, can be with
The amplification number of times of PCR instrument is reduced to a certain extent, so as to extend the service life of PCR instrument, the separation of embedded lid
Part be able to can be loaded in the case of covered, after being loaded and centrifuging, and the component of addition enters corresponding pipe shaft marker space,
Reduce the carry out difficulty of the reaction of secondary amplification, it is to avoid polluted caused by uncapping.
Particular embodiments described above, to the technical problem, technical scheme and beneficial effect of solution of the present utility model
It is further described, should be understood that and the foregoing is only specific embodiment of the utility model, not
For limiting the utility model, all any modifications within spirit of the present utility model and principle, made, equivalent substitution, change
Enter, should be included within protection domain of the present utility model.
Claims (5)
1. a kind of union component of cellular-type eight, it is characterised in that it includes eight reaction tubes and seven reaction pipe connections, eight
Reaction tube is linearly arranged, and is connected between two neighboring reaction tube by a reaction pipe connections, reaction tube includes reaction
Pipe main body, conical bottom pipe, dividing plate connecting pole, preceding dividing plate, right clapboard, rear bulkhead, left clapboard, the first reative cell, the second reative cell,
3rd reative cell, the 4th reative cell, embedded lid, reaction tube main body are fixedly connected with conical bottom pipe, and dividing plate connecting pole is located at
Reaction tube main body and conical bottom tube hub and dividing plate connecting pole bottom is fixedly connected with conical bottom bottom of the tube, preceding dividing plate one end with every
Panel connecting column is connected, and the preceding dividing plate other end is connected with reaction tube main body and conical bottom pipe respectively, and right clapboard one end is connected with dividing plate
Post is connected, and the right clapboard other end is connected with reaction tube main body and conical bottom pipe respectively, and rear bulkhead one end is connected with dividing plate connecting pole,
The rear bulkhead other end is connected with reaction tube main body and conical bottom pipe respectively, and left clapboard one end is connected with dividing plate connecting pole, left clapboard
The other end is connected with reaction tube main body and conical bottom pipe respectively, and preceding dividing plate, right clapboard, rear bulkhead, left clapboard are with dividing plate connecting pole
Centered on angle spaced apart ninety degrees from arrangement successively, preceding dividing plate, right clapboard, reaction tube main body, conical bottom pipe are interconnected to form first
Reative cell, right clapboard, rear bulkhead, reaction tube main body, conical bottom pipe are interconnected to form the second reative cell, rear bulkhead, left clapboard,
Reaction tube main body, conical bottom pipe are interconnected to form the 3rd reative cell, left clapboard, preceding dividing plate, reaction tube main body, conical bottom pipe phase
Connect to form the 4th reative cell, embedded lid is located at the top of preceding dividing plate and there is embedded lid cross to separate knot
Structure.
2. the union component of cellular-type eight as claimed in claim 1, it is characterised in that the embedded lid is close provided with one
The section of capping projection, reaction tube main body and conical bottom pipe is circle.
3. the union component of cellular-type eight as claimed in claim 1, it is characterised in that the embedded lid is close provided with one
Seal and sealing ring be located at the mouth of pipe of reaction tube main body with embedded lid mating connection.
4. the union component of cellular-type eight as claimed in claim 1, it is characterised in that provided with multiple inside the embedded lid
Connecting pole pod apertures.
5. the union component of cellular-type eight as claimed in claim 1, it is characterised in that the embedded lid is close provided with one
Envelope groove and reaction tube when closing embedded lid sealed groove coordinate with preceding dividing plate, right clapboard, rear bulkhead, left clapboard connect respectively
Connect.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720036975.9U CN206428253U (en) | 2017-01-13 | 2017-01-13 | The union component of cellular-type eight |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720036975.9U CN206428253U (en) | 2017-01-13 | 2017-01-13 | The union component of cellular-type eight |
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| Publication Number | Publication Date |
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| CN206428253U true CN206428253U (en) | 2017-08-22 |
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| CN201720036975.9U Active CN206428253U (en) | 2017-01-13 | 2017-01-13 | The union component of cellular-type eight |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108642146A (en) * | 2018-06-01 | 2018-10-12 | 广州双螺旋基因技术有限公司 | It is a kind of can pre-filled reagent S type microchannel formula nucleic acid amplification airtight reactor tubes |
| CN109718733A (en) * | 2019-02-25 | 2019-05-07 | 中国计量大学 | A kind of reaction unit and its working method with controllable outfield coupling function |
| CN111647497A (en) * | 2020-05-14 | 2020-09-11 | 南京达伯可特生物科技有限公司 | Multiple closed nucleic acid amplification product rapid detection device |
-
2017
- 2017-01-13 CN CN201720036975.9U patent/CN206428253U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108642146A (en) * | 2018-06-01 | 2018-10-12 | 广州双螺旋基因技术有限公司 | It is a kind of can pre-filled reagent S type microchannel formula nucleic acid amplification airtight reactor tubes |
| CN108642146B (en) * | 2018-06-01 | 2024-02-02 | 广州双螺旋基因技术有限公司 | S-type microtube nucleic acid amplification closed reaction tube capable of being preloaded with reagent |
| CN109718733A (en) * | 2019-02-25 | 2019-05-07 | 中国计量大学 | A kind of reaction unit and its working method with controllable outfield coupling function |
| CN109718733B (en) * | 2019-02-25 | 2023-09-12 | 中国计量大学 | Reaction device with adjustable external field coupling function and working method thereof |
| CN111647497A (en) * | 2020-05-14 | 2020-09-11 | 南京达伯可特生物科技有限公司 | Multiple closed nucleic acid amplification product rapid detection device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: Cellular -type eight allies oneself with pipe assembly Effective date of registration: 20180914 Granted publication date: 20170822 Pledgee: Beijing Leadman Biochemistry Co., Ltd. Pledgor: Ke Yi bio tech ltd, Shanghai Registration number: 2018310000060 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20200103 Granted publication date: 20170822 Pledgee: Beijing Leadman Biochemistry Co., Ltd. Pledgor: Ke Yi bio tech ltd, Shanghai Registration number: 2018310000060 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right |