CN205382165U - Closed pathogen detection card box - Google Patents
Closed pathogen detection card box Download PDFInfo
- Publication number
- CN205382165U CN205382165U CN201620139240.4U CN201620139240U CN205382165U CN 205382165 U CN205382165 U CN 205382165U CN 201620139240 U CN201620139240 U CN 201620139240U CN 205382165 U CN205382165 U CN 205382165U
- Authority
- CN
- China
- Prior art keywords
- cavity
- base
- prominent
- cylindrical sleeve
- slide plate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a closed pathogen detection card box, including a cavity, gleitbretter above the sealed cavity moves the liquid subassembly with the slide combines, and the buckle closure matches the base that combines with the cavity in addition. The whole process that this card box can collect pathogen detection includes nucleic acid extraction, and the increase detects and accomplishes in the confined environment, is applicable to automated control. Can effectively avoid the amplify pollution problem that exists in detecting and experiment operation personnel's infection scheduling problem such as pathogenic organism.
Description
Technical field
This utility model relates to a kind of closed pathogen detection cartridge, it is adaptable in the biochemical detection system of portable, automatization, belong to biology, analytical chemistry and medical science.
Background technology
Detection of nucleic acids has been widely used in the fields such as biological medicine.The whole process of traditional detection of nucleic acids (includes nucleic acid extraction, pcr amplification, detection) need to operate on different instruments to carry out, sample needs transfer repeatedly, this considerably increases sample and produce the probability of cross-contamination, the operating process of sampling detection of especially uncapping after pcr amplification is polluted.Thus causing that last testing result is inaccurate so that patient to be brought extremely serious harm.Although having been developed in that serial of methods is to prevent the cross-contamination of amplified production, as PCR operation is carried out strict zonal control, adopt the method for stopped pipe amplification and detection, utilize enzyme solution to eliminate the method etc. polluted, but experiment condition and operator are required higher by these methods, and anthropic factor impact is bigger.Also some are had can to realize the apparatus that sample preparation is integrated with detection in the market, such as the GeneXpert series of Cepheid company.These overwhelming majority are all external instruments, and price is sufficiently expensive, are not appropriate for promoting, particularly hold explosive place in diseases such as Africa.
Summary of the invention
Goal of the invention: this utility model purpose is in that offer one is portable, easily operates, it is adaptable to full automatic closed pathogen detection cartridge.
Technical scheme: the purpose of this utility model is achieved through the following technical solutions:
A kind of closed detection cartridge, including a cavity, at cavity top, buckle closure is set, the slide plate that closed cavity is set on buckle closure and the shifting liquid assembly being combined with slide plate, arranging in cavity bottom and mate the base combined with cavity, be arranged to U-shaped guide groove and prominent strip beam that described slide plate slides before and after described cavity on inwall, the spacing that two prominent strip beams are formed can limit described shifting liquid assembly and steadily move, before and after described cavity, inwall lower end is additionally provided with strip groove, respectively has a button hole simultaneously.
Described slide plate is provided with manhole.
The lateral wall, left and right of described cavity respectively arranges the oblique shallow slot of at least two.
The described liquid assembly that moves includes connecting the two-way connector of fluid suction head and plunger displacement pump pipeline, spring, cylindrical sleeve, cylindrical gland；Described spring is in cylindrical sleeve, and two-way connector inserts in spring, and two-way connector is pushed down by cylindrical gland, is limited in cylindrical sleeve by spring；The described two-way connector upper end moved in liquid assembly is tower head shape, pipeline UNICOM with plunger displacement pump, there is certain taper lower end, coordinate with fluid suction head, described cylindrical sleeve inwall is arranged with two guide grooves, described two-way connector symmetry both sides are provided with prominent top guide, coordinate with the guide groove of cylindrical sleeve inwall, the through hole on the cylindrical sleeve described slide plate of traverse in described shifting liquid assembly, the catching groove of described cylindrical sleeve both sides clasps the snap-on of cylindrical gland both sides, is pressed abd fixed on by slide plate between cylindrical sleeve and cylindrical gland.
Described base arranges some circular holes not of uniform size for laying the centrifuge tube of different size, base inner edge has some catching grooves, described base two foreign minister limit is also respectively provided with prominent strip and prominent button, described prominent strip and prominent button and coordinates with the strip groove of inwall lower end before and after cavity and button hole.
Cartridge according to claim 1, it is characterized in that: also include base tabletting, described base tabletting is arranged on chassis interior, base tabletting is provided with the hole corresponding with circular hole position on described base, the aperture that the aperture in described hole is slightly less than on base circular hole, press tab edge arranges the prominent button corresponding with the catching groove position on base inner edge.
Beneficial effect: pathogen detection cartridge described in the utility model can collect the whole process of pathogen detection and include nucleic acid extraction, amplification, it is detected in the environment of closing and completes, it is adaptable to Automated condtrol.The problem such as the pollution problem of existence in the augmentation detection such as pathogenic microorganism and the infection of experiment operator can be prevented effectively from.
Accompanying drawing explanation
Fig. 1 cartridge overall appearance figure；
Fig. 2 cartridge profile；
Fig. 3 a cartridge left view；
Fig. 3 b cartridge top view；
Fig. 4 cartridge moves liquid assembly assumption diagram；
Fig. 5 cartridge understructure figure.
Wherein: closed pathogen detection cartridge 1, slide plate 101, cavity 102, buckle closure 103, base 104, moves liquid assembly 105, centrifuge tube 106, U-shaped guide groove 201, prominent strip beam 202, oblique shallow slot 203, strip groove 204, cavity button hole 205, buckle closure is dashed forward button 301, base tabletting 401, base circular hole 402, tabletting circular hole 403, base catching groove 404, tabletting is dashed forward button 405, base highlights strip 406, and base is dashed forward button 407, two-way connector 501, cylindrical sleeve 502, cylindrical gland 503, spring 504, tower head 505, fluid suction head 506, prominent top guide 507.
Detailed description of the invention
Below in conjunction with accompanying drawing this utility model done and further explain.The following example is merely to illustrate this utility model, but is not used to limit practical range of the present utility model.
The closed pathogen detection cartridge 1 of one that this utility model relates to, main by slide plate 101, cavity 102, buckle closure 103, base 104, move liquid assembly 105, centrifuge tube 106 forms, described slide plate 101 is fixed in described shifting liquid assembly 105, move along the U-shaped guide groove 201 in cavity 102 by moving the mobile drive slide plate 101 of liquid assembly 105, move the top buckle closure 103 of liquid assembly 105 to be dashed forward by buckle closure and buckle 301 and clasp cavity 102 and vertical direction is fixed in cavity 102, horizontal direction then can move along cavity 102 upper edge edge and its internal prominent strip beam 202.Centrifuge tube 106 is put in the base circular hole 402 in base 104 after cutting off upper cover, base tabletting 401 clasps base catching groove 404 by the prominent button 405 of tabletting so that centrifuge tube 106 is fixed in base circular hole 402 by base tabletting 401.Base circular hole 402, tabletting circular hole 403, centrifuge tube pipe diameter size is adaptive.Adding required reagent by tabletting circular hole 403 in centrifuge tube 106, available masking foil seals all tabletting circular holes 403 of base tabletting 401, it is to avoid the pollution of reagent.Then being pushed into cavity in by the prominent strip 406 of base along the strip groove 204 of cavity 102 inwall by base 104, the prominent button 407 of base buckles in cavity button hole 205 so that base 104 is combined with cavity 102.So, slide plate 101, cavity 102, buckle closure 103, base 104, move liquid assembly 105, centrifuge tube 106 is formed for the environment closed.
The operations such as the detection of pathogen mainly needs to be accomplished that the mixing of liquid in closed cartridge, transfer.By the two-way connector 501 moved in liquid assembly 105 of press seal enclosed pathogen detection cartridge 1 under external control, two-way connector 501 compresses spring 504 through cylindrical gland 503, the prominent top guide 507 of two-way connector 501 moves down along the guide groove of cylindrical sleeve 502 inwall, the fluid suction head 506 of two-way connector 501 lower end can enter in the centrifuge tube 106 in base 104, control peripheral hardware plunger displacement pump imbibition or tapping, it may be achieved imbibition in centrifuge tube 106 and tapping.When the liquid that fluid suction head 506 is drawn needs to shift, removing the downforce of two-way connector 501, spring 504 resilience force of compression makes two-way connector 501 have the fluid suction head 506 of liquid to move up with suction, and spring 504 restores.Control to move liquid component level and move to destination locations, repeat above-mentioned operation, namely can realize moving the operation of liquid.The high frequency controlling outer plunger pump inhales tapping, it may be achieved to liquid piping and druming mixing operation in centrifuge tube.Other operations of pathogen detection can be realized by external drive.To sum up, coordinate external drive, imbibition, tapping can be completed in enclosed pathogen detection cartridge, move the actions such as liquid, piping and druming mixing, it is achieved the detection of pathogen.
Preferred implementation of the present utility model described in detail above; but; this utility model is not limited to the detail in above-mentioned embodiment; in technology concept of the present utility model; the technical solution of the utility model can being carried out multiple equivalents, these equivalents belong to protection domain of the present utility model.
Claims (6)
1. a closed detection cartridge, it is characterized in that: include a cavity, at cavity top, buckle closure is set, the slide plate that closed cavity is set on buckle closure and the shifting liquid assembly being combined with slide plate, arrange in cavity bottom and mate the base combined with cavity, inwall is arranged to before and after described cavity U-shaped guide groove and prominent strip beam that described slide plate slides, the spacing that two prominent strip beams are formed can limit described shifting liquid assembly and steadily move, before and after described cavity, inwall lower end is additionally provided with strip groove, respectively has a button hole simultaneously.
2. cartridge according to claim 1, it is characterised in that: described slide plate is provided with manhole.
3. cartridge according to claim 1, it is characterised in that: the lateral wall, left and right of described cavity respectively arranges the oblique shallow slot of at least two.
4. cartridge according to claim 1, it is characterised in that: the described liquid assembly that moves includes connecting the two-way connector of fluid suction head and plunger displacement pump pipeline, spring, cylindrical sleeve, cylindrical gland；Described spring is in cylindrical sleeve, and two-way connector inserts in spring, and two-way connector is pushed down by cylindrical gland, is limited in cylindrical sleeve by spring；The described two-way connector upper end moved in liquid assembly is tower head shape, pipeline UNICOM with plunger displacement pump, there is certain taper lower end, coordinate with fluid suction head, described cylindrical sleeve inwall is arranged with two guide grooves, described two-way connector symmetry both sides are provided with prominent top guide, coordinate with the guide groove of cylindrical sleeve inwall, the through hole on the cylindrical sleeve described slide plate of traverse in described shifting liquid assembly, the catching groove of described cylindrical sleeve both sides clasps the snap-on of cylindrical gland both sides, is pressed abd fixed on by slide plate between cylindrical sleeve and cylindrical gland.
5. cartridge according to claim 1, it is characterized in that: described base arranges some circular holes not of uniform size for laying the centrifuge tube of different size, base inner edge has some catching grooves, described base two foreign minister limit is also respectively provided with prominent strip and prominent button, described prominent strip and prominent button and coordinates with the strip groove of inwall lower end before and after cavity and button hole.
6. cartridge according to claim 1, it is characterized in that: also include base tabletting, described base tabletting is arranged on chassis interior, base tabletting is provided with the hole corresponding with circular hole position on described base, the aperture that the aperture in described hole is slightly less than on base circular hole, press tab edge arranges the prominent button corresponding with the catching groove position on base inner edge.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620139240.4U CN205382165U (en) | 2016-02-24 | 2016-02-24 | Closed pathogen detection card box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620139240.4U CN205382165U (en) | 2016-02-24 | 2016-02-24 | Closed pathogen detection card box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205382165U true CN205382165U (en) | 2016-07-13 |
Family
ID=56350894
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620139240.4U Active CN205382165U (en) | 2016-02-24 | 2016-02-24 | Closed pathogen detection card box |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205382165U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105586256A (en) * | 2016-02-24 | 2016-05-18 | 东南大学 | Closed pathogen detection cassette |
| KR20200098889A (en) * | 2019-02-13 | 2020-08-21 | 한국기계연구원 | Integrated molecular diagnosis cartridge |
| CN113150945A (en) * | 2021-04-14 | 2021-07-23 | 新羿制造科技（北京）有限公司 | Slide type cassette for nucleic acid extraction and PCR amplification detection |
-
2016
- 2016-02-24 CN CN201620139240.4U patent/CN205382165U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105586256A (en) * | 2016-02-24 | 2016-05-18 | 东南大学 | Closed pathogen detection cassette |
| CN105586256B (en) * | 2016-02-24 | 2017-11-03 | 东南大学 | A kind of closed pathogen detection cartridge |
| KR20200098889A (en) * | 2019-02-13 | 2020-08-21 | 한국기계연구원 | Integrated molecular diagnosis cartridge |
| KR102182376B1 (en) | 2019-02-13 | 2020-11-24 | 한국기계연구원 | Integrated molecular diagnosis cartridge |
| CN113150945A (en) * | 2021-04-14 | 2021-07-23 | 新羿制造科技（北京）有限公司 | Slide type cassette for nucleic acid extraction and PCR amplification detection |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11033894B2 (en) | System and apparatus for reactions | |
| CN205382165U (en) | Closed pathogen detection card box | |
| US10315195B2 (en) | Device, system and method processing a sample | |
| CN105586256B (en) | A kind of closed pathogen detection cartridge | |
| US11213232B2 (en) | Device for trapping an initial flow of blood | |
| CN104135927A (en) | Blood-sampling tube with integrated sensor device | |
| CN103114035B (en) | Device for fully-enclosed extraction, transfer and storage of nucleic acids | |
| WO2006039251A3 (en) | Method and apparatus for blood sampling | |
| CN104471369A (en) | Kit for nucleic acid extraction, and nucleic acid extractor | |
| CN103571740A (en) | Device integrated with extraction, amplification and detection of nucleic acid | |
| CN104818213B (en) | Sliding type closed pathogen detection card box | |
| CN203668357U (en) | Device integrating nucleic acid extraction, amplification and detection | |
| CN108139003A (en) | For at least one product withdraw pipe to be connected to the equipment for the device for receiving product, such as the instrument from extraction nucleic acid | |
| CN206768084U (en) | A kind of PCR plate with PCR lids | |
| CN218435756U (en) | Nucleic acid detection micro-fluidic chip for in-situ capture and amplification and nucleic acid detector | |
| CN103923817B (en) | A kind of micro-fluidic DNA extraction system of linear pattern detected towards PCR | |
| CN211954846U (en) | Quantitative injector for circulating system | |
| CN204832223U (en) | Biological sample preparation device of magnetic drive | |
| CN105158492A (en) | Automatic pipetting device | |
| CN219194987U (en) | Nucleic acid extraction device | |
| CN205176046U (en) | Automatic liquid -transfering device | |
| CN210037311U (en) | Sampling device for clinical laboratory | |
| CN221296897U (en) | Detection device | |
| CN202182897U (en) | Injection steel needle of automatic sampling gun | |
| CN118086044A (en) | Nucleic acid extraction device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |