CN105586256B - A kind of closed pathogen detection cartridge - Google Patents
A kind of closed pathogen detection cartridge Download PDFInfo
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- CN105586256B CN105586256B CN201610102868.1A CN201610102868A CN105586256B CN 105586256 B CN105586256 B CN 105586256B CN 201610102868 A CN201610102868 A CN 201610102868A CN 105586256 B CN105586256 B CN 105586256B
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- base
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- cylindrical sleeve
- liquid relief
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
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Abstract
The present invention provides the slide plate above a kind of closed pathogen detection cartridge, including a cavity, closed cavity, and the liquid relief component combined with slide, buckle closure also has the base for matching and combining with cavity.The whole process that this cartridge can collect pathogen detection includes nucleic acid extraction, and amplification is detected in the environment of closing and completed, it is adaptable to Automated condtrol.The problems such as pollution problem present in the augmentation detections such as pathogenic microorganism and infection of experiment operator can be prevented effectively from.
Description
Technical field
The present invention relates to a kind of closed pathogen detection cartridge, it is adaptable to portable, automation biochemical detection system
In, belong to biology, analytical chemistry and medical science.
Background technology
Detection of nucleic acids is had been widely used in the fields such as biological medicine.Traditional detection of nucleic acids whole process (including
Nucleic acid extraction, PCR amplifications, detection) need to operate progress on different instruments, sample is needed to shift multiple, and this is greatly increased
Sample produces the probability of cross pollution, is especially polluted from the operating process for uncapping sampling detection after PCR amplifications.From
And cause last testing result inaccurate so that extremely serious harm is all brought to patient.Although having been developed a series of
Method is such as operated to PCR to prevent the cross pollution of amplified production and carries out strict zonal control, expanded and detection using stopped pipe
Method, eliminate using enzyme solution method etc. of pollution, but these methods require higher to experiment condition and operating personnel,
And human factor influence is larger.Also there are some to realize sample preparation and the integrated apparatus detected in the market,
Such as the GeneXpert series of Cepheid companies.These overwhelming majority are all external instruments, and price is sufficiently expensive, is not appropriate for
Promote, particularly hold explosive place in diseases such as Africa.
The content of the invention
Goal of the invention：It is a kind of portable, easy to operate present invention aims at providing, it is adaptable to full automatic closed pathogen
Detect cartridge.
Technical scheme：The purpose of the present invention is achieved through the following technical solutions：
A kind of closed detection cartridge, including a cavity, buckle closure is set at the top of cavity, closing chamber is set on buckle closure
The slide plate of body and the liquid relief component combined with slide plate, are set in cavity bottom before the base for matching and combining with cavity, the cavity
U-shaped guide groove and prominent strip beam that the slide plate is slided are provided on inwall afterwards, the spacing of two protrusion strip beam formation can be limited
The fixed liquid relief component is steadily moved, and inwall lower end is additionally provided with strip groove before and after the cavity, while being respectively provided with a button hole.
The slide plate is provided with manhole.
The left and right lateral wall of the cavity respectively sets at least two oblique shallow slots.
Two-way connector of the described liquid relief component including connection fluid suction head and plunger pump conduit, spring, cylindrical sleeve,
Cylindrical gland；The spring is in cylindrical sleeve, and in two-way connector insertion spring, cylindrical gland is by two-way connector
Push down, spring is limited in cylindrical sleeve；Two-way connector upper end in described liquid relief component is tower head shape, with plunger
There is certain taper the pipeline UNICOM of pump, lower end, coordinates with fluid suction head, and the cylindrical sleeve inwall is arranged with two guide grooves,
The described symmetrical both sides of two-way connector are provided with prominent top guide, coordinate with the guide groove of cylindrical sleeve inwall, the liquid relief component
In cylindrical sleeve through the through hole on the slide plate, the catching groove of the cylindrical sleeve both sides clasps cylindrical gland both sides
Snap-on, slide plate is pressed abd fixed between cylindrical sleeve and cylindrical gland.
Some circular holes not of uniform size are set to be used on the centrifuge tube for laying different size, base inner edge on described base
There are some catching grooves, the foreign minister side of base two is also respectively provided with before prominent strip and prominent button, the prominent strip and prominent button and cavity
The strip groove of inwall lower end and button hole coordinate afterwards.
Cartridge according to claim 1, it is characterised in that：Also include base tabletting, the base tabletting is arranged on
Chassis interior, is provided with hole corresponding with circular hole position on the base, the aperture in the hole is slightly less than base in base tabletting
The aperture of upper circular hole, press tab edge sets the prominent button corresponding with the catching groove position on base inner edge.
Beneficial effect：The whole process that pathogen detection cartridge of the present invention can collect pathogen detection is carried including nucleic acid
Take, expand, be detected in the environment of closing and complete, it is adaptable to Automated condtrol.The amplification inspection such as pathogenic microorganism can be prevented effectively from
The problems such as pollution problem present in survey and the infection of experiment operator.
Brief description of the drawings
Fig. 1 cartridge overall appearance figures；
Fig. 2 cartridge profiles；
Fig. 3 a cartridge left views；
Fig. 3 b cartridge top views；
Fig. 4 cartridge liquid relief assembly assumption diagrams；
Fig. 5 cartridge understructure figures.
Wherein：Closed pathogen detection cartridge 1, slide plate 101, cavity 102, buckle closure 103, base 104, liquid relief component
105, centrifuge tube 106, U-shaped guide groove 201, prominent strip beam 202, oblique shallow slot 203, strip groove 204, cavity button hole 205, buckle closure
Prominent button 301, base tabletting 401, base circular hole 402, tabletting circular hole 403, base catching groove 404, the prominent button 405 of tabletting, base is protruded
Strip 406, the prominent button 407 of base, two-way connector 501, cylindrical sleeve 502, cylindrical gland 503, spring 504, tower head
505, fluid suction head 506, prominent top guide 507
Embodiment
The present invention is done below in conjunction with the accompanying drawings and further explained.The following example is merely to illustrate the present invention, but simultaneously
It is not used to limit the practical range of the present invention.
A kind of closed pathogen detection cartridge 1 of the present invention, mainly by slide plate 101, cavity 102, buckle closure 103,
Base 104, liquid relief component 105, centrifuge tube 106 is constituted, and the slide plate 101 is fixed in the liquid relief component 105, passes through liquid relief
The mobile drive slide plate 101 of component 105 is moved along the U-shaped guide groove 201 in cavity 102, the top buckle closure of liquid relief component 105
103 clasp cavity 102 and vertical direction is fixed in cavity 102 by the prominent button 301 of buckle closure, and horizontal direction then can be along cavity
102 upper edge edges and its internal protrusion strip beam 202 are moved.Centrifuge tube 106 cuts off the base being put into after lid in base 104
In circular hole 402, base tabletting 401 clasps base catching groove 404 by the prominent button 405 of tabletting so that base tabletting 401 is by centrifuge tube
106 are fixed on 402 in base circular hole.Base circular hole 402, tabletting circular hole 403, centrifuge tube pipe diameter size adaptation.Justified by tabletting
Hole 403 adds required reagent into centrifuge tube 106, and all tabletting circular holes 403 of base tabletting 401 can be sealed with masking foil, are kept away
It is excused from an examination the pollution of agent.Then base 104 is protruded into strip groove 204 of the strip 406 along the inwall of cavity 102 by base to be pushed into
Into cavity, the prominent button 407 of base is buckled into cavity button hole 205 so that base 104 is combined with cavity 102.So, slide plate 101,
Cavity 102, buckle closure 103, base 104, liquid relief component 105, centrifuge tube 106 just forms the environment of closing.
The detection of pathogen is main in closed cartridge to be needed to be accomplished that the mixing of liquid, and transfer etc. is operated.Pass through
Outside control pushes the two-way connector 501 in the liquid relief component 105 of closed pathogen detection cartridge 1, two-way connector 501
Through the cylindrical compression spring 504 of gland 503, the protrusion top guide 507 of two-way connector 501 is along the inwall of cylindrical sleeve 502
Guide groove move down, the fluid suction head 506 of the lower end of two-way connector 501 can enter in the centrifuge tube 106 in base 104, control
The imbibition of peripheral hardware plunger pump or tapping, the imbibition and tapping that may be implemented in centrifuge tube 106.The liquid drawn when fluid suction head 506
When needing transfer, remove the lower pressure of two-way connector 501, the screen resilience of spring 504 of compression cause two-way connector 501 with
The fluid suction head 506 that inhaling has liquid is moved up, and spring 504 restores.Control liquid relief component level is moved in destination locations, repetition
The operation stated, you can to realize the operation of liquid relief.Control the high frequency of outer plunger pump to inhale tapping, may be implemented in centrifuge tube to liquid
Body piping and druming mixes operation.Other operations of pathogen detection can be realized by external drive.To sum up, external drive is coordinated, in envelope
The pathogen detection cartridge of enclosed can complete imbibition, and tapping, liquid relief, piping and druming mixing etc. is acted, and realizes the detection of pathogen.
The preferred embodiment of the present invention described in detail above, still, the present invention are not limited in above-mentioned embodiment
Detail, in the range of the technology design of the present invention, a variety of equivalents can be carried out to technical scheme, this
A little equivalents belong to protection scope of the present invention.
Claims (1)
1. a kind of closed detection cartridge, it is characterised in that：Including a cavity, buckle closure is set at the top of cavity, on buckle closure
The liquid relief component for setting the slide plate of closed cavity and being combined with slide plate, the base for matching and combining with cavity is set in cavity bottom,
U-shaped guide groove and prominent strip beam that the slide plate is slided are provided for before and after the cavity on inwall, two protrusion strip beams are formed
Spacing can limit the liquid relief component and steadily move, inwall lower end is additionally provided with strip groove before and after the cavity, while respectively opening
There is a button hole；
Left and right lateral wall of the slide plate provided with cavity described in manhole respectively sets at least two oblique shallow slots；
Described liquid relief component includes the two-way connector of connection fluid suction head and plunger pump conduit, spring, cylindrical sleeve, cylinder
Shape gland；The spring is in cylindrical sleeve, and in two-way connector insertion spring, cylindrical gland is by two-way connector pressure
Firmly, spring is limited in cylindrical sleeve；Two-way connector upper end in described liquid relief component is tower head shape, with plunger pump
Pipeline UNICOM, there is certain taper lower end, coordinates with fluid suction head, and the cylindrical sleeve inwall is arranged with two guide grooves, institute
The symmetrical both sides of two-way connector stated are provided with prominent top guide, coordinate with the guide groove of cylindrical sleeve inwall, in the liquid relief component
Cylindrical sleeve through the through hole on the slide plate, the catching groove of the cylindrical sleeve both sides clasps cylindrical gland both sides
Snap-on, slide plate is pressed abd fixed between cylindrical sleeve and cylindrical gland；
Some circular holes not of uniform size are set to be used for the centrifuge tube for laying different size on described base, if having on base inner edge
Dry catching groove, the foreign minister side of base two is also respectively provided with prominent strip and prominent button, the prominent strip and prominent button with it is interior before and after cavity
The strip groove and button hole of wall lower end coordinate；
Also include base tabletting, the base tabletting is arranged on chassis interior, be provided with base tabletting and circle on the base
The corresponding hole in hole site, the aperture in hole is slightly less than the aperture of circular hole on base, and press tab edge is set and the catching groove on base inner edge
The corresponding prominent button in position；
In use, the mixing and transfer operation of liquid are realized in the detection of pathogen in closed cartridge；By under outside control
Two-way connector in the liquid relief component of press seal enclosed pathogen detection cartridge, two-way connector passes through cylindrical gland compression bullet
Spring, the guide groove of the protrusion top guide of two-way connector along cylindrical sleeve inwall is moved down, the imbibition of two-way connector lower end
Head enters in the centrifuge tube in base, control peripheral hardware plunger pump imbibition or tapping, realizes imbibition and tapping in centrifuge tube；
When the liquid that fluid suction head is drawn needs transfer, the lower pressure of two-way connector is removed, the recoil of spring power of compression causes two-way
The fluid suction head that connector has liquid with inhaling is moved up, and spring restores；Control liquid relief component level is moved to destination locations, weight
Multiple above-mentioned operation, realizes the operation of liquid relief；The high frequency of outer plunger pump is controlled to inhale tapping, realization is blown liquid in centrifuge tube
Beat and mix operation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610102868.1A CN105586256B (en) | 2016-02-24 | 2016-02-24 | A kind of closed pathogen detection cartridge |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610102868.1A CN105586256B (en) | 2016-02-24 | 2016-02-24 | A kind of closed pathogen detection cartridge |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105586256A CN105586256A (en) | 2016-05-18 |
| CN105586256B true CN105586256B (en) | 2017-11-03 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610102868.1A Active CN105586256B (en) | 2016-02-24 | 2016-02-24 | A kind of closed pathogen detection cartridge |
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| CN (1) | CN105586256B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106353492B (en) * | 2016-10-13 | 2019-03-05 | 东南大学 | One kind screening cartridge device for tumour cell nucleic acid aptamers |
| CN106318865B (en) * | 2016-10-25 | 2018-03-16 | 西安交通大学 | A kind of nucleic acid extraction and the portable unit of gene magnification |
| CN107964504B (en) * | 2017-10-28 | 2023-10-27 | 深圳职业技术学院 | Closed sample integrated detection device and detection method |
| CN113150945B (en) * | 2021-04-14 | 2023-01-10 | 新羿制造科技（北京）有限公司 | Slide-type nucleic acid extraction and PCR amplification detection card box |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203663858U (en) * | 2013-11-26 | 2014-06-25 | 东南大学 | Multi-channel pipette |
| CN104818213A (en) * | 2015-05-14 | 2015-08-05 | 东南大学 | Sliding type closed pathogen detection card box |
| CN205382165U (en) * | 2016-02-24 | 2016-07-13 | 东南大学 | Closed pathogen detection card box |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5707253B2 (en) * | 2011-06-27 | 2015-04-22 | 株式会社日立ハイテクノロジーズ | Cold reagent storage and nucleic acid analyzer |
-
2016
- 2016-02-24 CN CN201610102868.1A patent/CN105586256B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203663858U (en) * | 2013-11-26 | 2014-06-25 | 东南大学 | Multi-channel pipette |
| CN104818213A (en) * | 2015-05-14 | 2015-08-05 | 东南大学 | Sliding type closed pathogen detection card box |
| CN205382165U (en) * | 2016-02-24 | 2016-07-13 | 东南大学 | Closed pathogen detection card box |
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| Publication number | Publication date |
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| CN105586256A (en) | 2016-05-18 |
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