CN112094729A - Rotary liquid-transferring type totally-enclosed nucleic acid detection card box - Google Patents
Rotary liquid-transferring type totally-enclosed nucleic acid detection card box Download PDFInfo
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- CN112094729A CN112094729A CN202011031726.3A CN202011031726A CN112094729A CN 112094729 A CN112094729 A CN 112094729A CN 202011031726 A CN202011031726 A CN 202011031726A CN 112094729 A CN112094729 A CN 112094729A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Abstract
The invention discloses a rotary liquid-transferring type totally-closed nucleic acid detection card box which comprises a hollow cavity, a sleeve positioned in the middle of the cavity, a base matched with the cavity, a vertical guide rod penetrating through the center of the base, a spring positioned in the vertical guide rod, a liquid-transferring assembly capable of sliding up and down and rotating horizontally under the limitation of the sleeve and the vertical guide rod, a plurality of centrifugal tubes of different specifications and a gland for fixing the centrifugal tubes, wherein the centrifugal tubes are arranged on the base. The invention can realize nucleic acid extraction and multiple real-time fluorescence PCR in a closed box body formed by the components in a pipetting mode, thereby realizing full-automatic nucleic acid detection, effectively avoiding the problems of cross contamination and the like in the amplification detection process and improving the environmental adaptability of nucleic acid detection.
Description
Technical Field
The invention relates to the technical field of biochemical and medical detection, in particular to a rotary liquid-transferring type totally-closed nucleic acid detection card box.
Background
Nucleic acid detection is widely applied in various fields of biomedicine, and particularly in detection of infectious pathogens, the nucleic acid detection result is an important judgment standard for determining diagnosis. Traditional nucleic acid testing process is loaded down with trivial details, including steps such as nucleic acid extraction, PCR amplification, result detection, need operate the completion on different instrument and equipment, and this has not only provided higher requirement to experimenter and experimental environment, has more increased the sample and has taken place cross contamination's risk in many times transfer process to influence the accuracy of testing result. At present, only a few foreign companies develop commercial instruments with integration of nucleic acid extraction and amplification detection in the market, but most of the instruments are very expensive and are not suitable for popularization in regions with laggard medical resources.
Disclosure of Invention
Aiming at the problems of complicated steps, time and labor consumption, the need of matching of multiple instruments and the like existing in the field of nucleic acid detection, the invention provides the closed card box which is simple and portable, is simple to operate and can realize full-automatic nucleic acid detection.
The technical scheme of the invention is as follows: a rotary liquid-transferring type totally-enclosed nucleic acid detection card box comprises a cylindrical hollow cavity and a disc-shaped base which is arranged at the bottom of the hollow cavity and is matched with the hollow cavity;
a cylindrical sleeve is arranged in the hollow cavity, penetrates through the hollow cavity and extends towards the two ends of the upper outer side and the lower outer side of the hollow cavity.
Further, the base comprises a base chassis, a base side wall arranged at the periphery of the base chassis and a gland arranged on the base;
a round hole is arranged in the center of the gland, and a plurality of gland circumferential holes with different sizes and diameters are arranged around the round hole;
a base center hole is formed in the center of the base, a raised thin-wall cylinder is arranged on the outer side of the base center hole, and a plurality of base circumferential holes with different sizes and diameters are further arranged around the base center hole;
a plurality of centrifugal tubes are arranged in the circumferential holes of the bases;
at least four convex buckles are arranged on the outer wall of the gland, and at least four buckle grooves matched with the convex buckles in position are formed in the side wall of the base.
Furthermore, an integrated folded pipetting assembly matched with the cylindrical sleeve is arranged in the cylindrical sleeve,
the integral folded liquid-transferring assembly comprises a tower head at the top, a straight handle in the cylindrical shape at the middle part and a liquid-transferring assembly base provided with 4 fan-shaped hollows at the bottom, wherein a folded liquid-absorbing head adapter is arranged on the liquid-transferring assembly base, the folded liquid-absorbing head adapter comprises a conical boss, and a liquid-absorbing head is arranged on the conical boss;
an air passage is arranged in the integrated folded liquid-transferring assembly and is communicated with an external pipeline arranged on the top tower head and a liquid-absorbing head arranged on the conical boss;
a vertical guide rod is arranged at the lower part of the pipetting assembly base, penetrates between the round hole and the center hole of the base and comprises a pipetting assembly base, a guide rod body in a hollow structure and a guide rod base of a hollow cylinder at the lower part, wherein the upper part of the pipetting assembly base is connected with the guide rod body;
4 clamping grooves matched with the fan-shaped hollow-out liquid transfer assembly base are formed in the guide rod body; a plurality of fan-shaped grooves are arranged on the guide rod base; a spring is arranged inside the guide rod body;
the diameter of the inner wall of the guide rod base is matched with the outer diameter of the thin-wall cylinder protruding from the base, and a circle of groove is formed in the corresponding position.
Furthermore, the gland is clamped in the base, and the diameter of the gland is smaller than that of the base; the position of the round hole is matched with the position of the central hole of the base, and the position of the circumferential hole of the gland is matched with the position of the circumferential hole of the base.
Furthermore, the inner diameter of the cylindrical sleeve is matched with the outer diameter of a straight handle in the integrated zigzag liquid-transfering assembly, the outer diameter of the upper end of the straight handle is smaller than the inner diameter of the cylindrical sleeve, and the straight handle can slide up and down or rotate horizontally along the inner wall of the cylindrical sleeve;
the caliber of the top opening of the cylindrical sleeve is slightly smaller than the inner diameter of the cylinder body of the cylindrical sleeve.
Furthermore, the diameter of the base chassis is slightly larger than the outer diameter of the side wall of the base, and the diameter of the base chassis is matched with the outer diameter and the inner diameter of the hollow cavity respectively;
a circle of thin-wall cylinder which protrudes outwards is arranged on the bottom surface of the base chassis around the circumferential hole of the base, and a circle of protrusion is arranged on the outer side of the cylinder.
Further, the centrifuging tube includes 1.5mL volume and 200 uL volume two kinds, the centrifuging tube wear to establish in the base circumference hole, the aperture of base round hole slightly is less than the bore of the mouth of pipe of centrifuging tube, the fixed top that sets up at the centrifuging tube of gland.
The invention has the beneficial effects that: the nucleic acid detection card box forms a closed environment by assembling and combining various components, can realize all processes of nucleic acid extraction, amplification, detection and the like in the card box, is suitable for automatic control due to the structural design of the card box, does not need manual intervention in the whole detection process, does not need sample transfer, can effectively avoid the problems of cross contamination, infection of experiment operators and the like in the detection process, and improves the detection efficiency and the accuracy of detection results.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a cross-sectional view of the present invention;
FIG. 3 is a bottom view of the present invention;
FIG. 4 is a schematic view of the internal structure of the present invention;
FIG. 5 is a schematic view of the structure of the base of the present invention;
FIG. 6 is a schematic view of the integral accordion pipette assembly of the present invention;
FIG. 7 is a schematic view of the construction of the vertical guide bar of the present invention;
wherein: 100 is a hollow cavity, 101 is a cylindrical sleeve,
200 is a base, 201 is a gland, 202 is a base chassis, 203 is a base side wall, 204 is a buckle groove, 205 is a convex buckle, 206 is a base center hole, 207 is a base circumference hole, 208 is a gland circumference hole, and 209 is a convex thin-wall cylinder;
300 is an integrated folded pipetting assembly, 301 is a straight handle, 302 is a pipetting assembly base, 303 is a conical boss, 304 is a tower head, 305 is an air channel, and 306 is a pipetting head;
400 is a vertical guide rod, 401 is a guide rod body, 402 is a guide rod base, 403 is a fan-shaped groove, and 404 is a spring;
500 is a centrifuge tube.
Detailed Description
In order to more clearly illustrate the technical solution of the present invention, the following detailed description is made with reference to the accompanying drawings:
a rotary liquid-transfering type totally-enclosed nucleic acid detecting card box comprises a cylindrical hollow cavity 100 and a disc-shaped base 200 which is arranged at the bottom of the hollow cavity 100 and is matched with the hollow cavity 100;
a cylindrical sleeve 101 is installed inside the hollow cavity 100, and the cylindrical sleeve 101 penetrates through the inside of the hollow cavity 100 and extends to two ends of the upper and lower outer sides of the hollow cavity 100.
Further, the base 200 comprises a base chassis 202, a base sidewall 203 disposed at the periphery of the base chassis 202, and a gland 201 disposed on the base 200;
a round hole is arranged in the center of the gland 201, and a plurality of gland circumferential holes 208 with different sizes and diameters are arranged around the round hole;
a base center hole 206 is arranged in the center of the base 200, a raised thin-wall cylinder 209 is arranged on the outer side of the base center hole 206, and a plurality of base circumferential holes 207 with different sizes and diameters are arranged around the base center hole 206;
a plurality of centrifuge tubes 500 are arranged in the circumferential holes 207 of the bases;
at least four convex buckles 205 are arranged on the outer wall of the gland 201, and at least four buckle grooves 204 matched with the convex buckles 205 in position are arranged on the side wall 203 of the base.
Further, an integrated zigzag pipetting assembly 300 matched with the cylindrical sleeve 101 is arranged in the cylindrical sleeve 101,
the integrated folded liquid-transfering component 300 comprises a tower head 304 at the top, a straight handle 301 with a cylindrical middle part and a liquid-transfering component base 302 with 4 fan-shaped hollows at the bottom, wherein a folded liquid-transfering head adapter is arranged on the liquid-transfering component base 302, the folded liquid-transfering head adapter comprises a conical boss 303, and a liquid-transfering head 306 is arranged on the conical boss 303;
an air channel 300 is arranged inside the integrated zigzag liquid-transfering component 300, and the air channel 300 is communicated with an external pipeline arranged on a top tower head 304 and a liquid-transfering head 306 arranged on a conical boss 303;
a vertical guide rod 400 is arranged at the lower part of the pipetting assembly base 302, the vertical guide rod 400 is arranged between the circular hole and the base center hole 206 in a penetrating way, and the vertical guide rod 400 comprises a pipetting assembly base 302, a guide rod body 401 with a hollow structure and a guide rod base 402 with a hollow cylinder at the lower part, wherein the upper part of the pipetting assembly base 302 is connected with the guide rod body;
4 clamping grooves matched with the fan-shaped hollow-out liquid transfer assembly base 302 are formed in the guide rod body 401; a plurality of fan-shaped grooves 403 are arranged on the guide rod base 402; a spring 404 is arranged inside the guide rod body 401;
the diameter of the inner wall of the guide rod base 402 is matched with the outer diameter of the thin-wall cylinder 209 protruding from the base 200, and a circle of groove is arranged at the corresponding position.
Further, the gland 201 is clamped inside the base 200, and the diameter of the gland 201 is smaller than that of the base 200; the position of the circular hole is matched with that of the base center hole 206, and the position of the gland circumferential hole 208 is matched with that of the base circumferential hole 207.
Further, the inner diameter of the cylindrical sleeve 101 is matched with the outer diameter of the straight handle 301 in the integral zigzag liquid-transfering assembly 300, the outer diameter of the upper end of the straight handle 301 is smaller than the inner diameter of the cylindrical sleeve 101, and the straight handle 301 can slide up and down or rotate horizontally along the inner wall of the cylindrical sleeve 101;
the caliber of the top opening of the cylindrical sleeve 101 is slightly smaller than the inner diameter of the cylinder body of the cylindrical sleeve 101.
Further, the diameter of the base chassis 202 is slightly larger than the outer diameter of the base sidewall 203, and the two are respectively matched with the outer diameter and the inner diameter of the hollow cavity 100;
a circle of thin-wall cylinders protruding outwards are arranged on the bottom surface of the base chassis 202 around the base circumferential hole 207, and a circle of protrusions are arranged on the outer sides of the cylinders.
Further, centrifuge tube 500 includes 1.5mL volume and 200 μ L volume two kinds, centrifuge tube 500 wear to establish in base circumference hole 207, the aperture of base round hole 207 slightly is less than the bore of centrifuge tube 500's mouth of pipe, gland 201 is fixed to be set up in centrifuge tube 500's top.
Specifically, the invention relates to a rotary liquid-transferring type totally-enclosed nucleic acid detection card box, which comprises a cylindrical hollow cavity 100, a disc-shaped base 200 matched with the hollow cavity 100, a gland 201, an integrated folded liquid-transferring component 300, a vertical guide rod 400, a spring 400, a plurality of centrifuge tubes 500 with different specifications (two specifications) and the like, wherein the devices form a closed cavity together;
a cylindrical sleeve 101 is arranged at the top of the cylindrical hollow cavity 100, the cylindrical sleeve 101 penetrates through the top of the hollow cavity 100 and extends towards the inner side and the outer side, the inner diameter of the cylindrical sleeve 101 is matched with the outer diameter of a cylindrical straight handle 301 of the integrated folded pipetting assembly 300, the straight handle 301 can slide up and down or rotate horizontally along the inner wall of the cylindrical sleeve 101, and the top opening of the cylindrical sleeve 101 is slightly smaller than the inner diameter of the cylindrical sleeve 101 and is used for limiting the integrated folded pipetting assembly 300 in the cartridge; a circle of groove is arranged on the inner wall of the hollow cavity 100 near the bottom and matched with a circle of bulge arranged at the corresponding position of the outer wall of the disc-shaped base 200, so that the close combination of the two is ensured.
The disc-shaped base 200 comprises a base chassis 202 and a base side wall 203, the diameter of the base chassis 202 is slightly larger than the outer diameter of the base side wall 203, and the outer diameter and the inner diameter of the base chassis are respectively matched with the outer diameter and the inner diameter of the hollow cavity 100; four buckle grooves 204 are arranged on the side wall 203 of the base and are matched with four convex buckles 205 arranged on the edge of the gland 201, so that the gland 201 can be tightly buckled on the disc-shaped base 200; the chassis 200 is provided with a plurality of base circumferential holes 207 with different sizes around the circle center, and is used for installing centrifuge tubes 500 with different specifications; a base circumferential hole 207 is formed in the center of the base chassis 202, a circle of thin-wall cylinders 209 protruding outwards are arranged on the bottom surface of the base 200 around the base central hole 206, and a circle of protrusions are arranged on the outer sides of the protruding thin-wall cylinders 209;
the vertical guide rod 400 comprises a guide rod body 401 and a guide rod base 402, the guide rod body 401 is of a hollow structure, a spring 404 is installed in the middle of the guide rod body 401, 4 pairwise opposite grooves are cut in the upper portion of the guide rod body 401, and the lower portion of the guide rod body 401 is a thin-walled cylinder with a bottom; the guide rod base 402 is a hollow cylinder with a bottom and is connected with the guide rod body 401, 3 fan-shaped grooves 403 are formed in the bottom of the guide rod base 402, the diameter of the inner wall of the guide rod base 402 is matched with the outer diameter of the convex thin-wall cylinder 209 on the outer side of the base 200, and a circle of groove is formed in the corresponding position, so that the guide rod base 402 can be buckled into a protrusion of the base 200 and can horizontally rotate under the action of external force;
the integrated folded liquid-transferring assembly 300 comprises a cylindrical straight handle 301, a liquid-transferring assembly base 302 with 4 fan-shaped hollowed parts and a folded liquid-absorbing head adapter; the diameter of the upper end of the cylindrical straight handle 301 is smaller, the cylindrical straight handle can extend out of the cylindrical sleeve 101, a tower head 304 with a tower head-shaped structure is arranged at the top end of the cylindrical straight handle and can be connected with a plunger pump pipeline, and the main body of the cylindrical straight handle 301 can slide up and down or horizontally rotate in the cylindrical sleeve 101; the fan-shaped hollow is matched with the shape of the upper part of the vertical guide rod 400 after a groove is cut off, the pipetting assembly base 302 can be clamped into the vertical guide rod 400, can slide up and down along the vertical guide rod 400 and can horizontally rotate under the driving of the vertical guide rod 400; the lower end of the fold-shaped liquid suction head adapter is provided with a conical boss 303 with a certain taper for installing and fixing a liquid suction head 306; an air channel 305 is arranged inside the integrated zigzag pipetting assembly 300 and is used for connecting an external plunger pump pipeline and a pipetting head;
a rotary liquid-transfer type totally-enclosed nucleic acid detection card box can realize the full-automatic nucleic acid extraction and the real-time fluorescence PCR detection in the enclosed environment formed by the components together under the drive of an external instrument; before use, all reagents required for detection are preloaded in each centrifuge tube 500, the centrifuge tube 500 is inserted into the base circumferential hole 207, the position of the gland circumferential hole 208 corresponds to the base circumferential hole 207, the aperture of the gland circumferential hole is slightly smaller than the aperture of the centrifuge tube 500, the gland 201 buckles the 4 buckle grooves 204 of the base through the 4 convex buckles 205 on the side surface, and the centrifuge tube 500 is fixed on the base 200; before use, all gland circumferential holes 208 of the gland 201 can be sealed by tinfoil paper, so that reagent leakage and pollution are prevented; the pipetting assembly base 302 of the integrated zigzag pipetting assembly 300 is inserted into the upper end of the guide rod body 401 of the vertical guide rod 400, the straight handle 301 is inserted into the cylindrical sleeve 101 from inside to outside, the part with smaller diameter at the top of the straight handle 301 extends out of the cylindrical sleeve 101, the tower head 304 positioned at the top end of the straight handle 301 is connected with an external plunger pump pipeline, and the liquid suction head 306 is arranged on the conical boss 303 positioned at the other end of the integrated zigzag pipetting assembly 300; inside vertical guide rod 400 got into the card box through base centre bore 206, guide rod base 402 card was gone into the outside cylindrical protrusion of base chassis 202, and restriction vertical guide rod 400 can only be rotary motion, is provided with a spring 404 in the middle of the guide rod body of rod 401, and when the integral type folded shape moved liquid subassembly 300 down motion under the exogenic action, spring 404 shrink, and when the exogenic action was removed, the integral type folded shape moved liquid subassembly 300 up motion under the effect of spring 404 elasticity.
In the detection process, the card box has the functions of realizing the uniform mixing of liquid and the transfer among different centrifuge tubes 500 by the integrated zigzag liquid-transferring assembly 300 under the driving of an external instrument; an external instrument drives the vertical guide rod 400 to rotate through a structure matched with the fan-shaped groove 403 at the bottom of the vertical guide rod 400, the vertical guide rod 400 can drive the integrated folded liquid transfer assembly 300 clamped into the guide rod body 401 to rotate, so that the liquid suction head 306 moves to the position above the centrifugal tube 500 to be sucked, the vertical guide rod 400 arranged by the external instrument presses down the straight handle 301 in the integrated folded liquid transfer assembly 300, the integrated folded liquid transfer assembly 300 moves downwards along the cylindrical sleeve 101 and the guide rod body 401 of the vertical guide rod 400, the spring 404 is compressed, the liquid suction head 306 enters the position below the liquid level of the centrifugal tube 500 to be sucked, the external plunger pump performs suction action, liquid enters the liquid suction head 306, external force is removed, the integrated folded liquid transfer assembly 300 is lifted upwards under the resilience force of the spring 404, the liquid suction head 306 leaves the centrifugal tube 500 to be sucked, the vertical guide rod 400 rotates under the external force to drive the liquid suction head 306 to move, the integral folded liquid-transferring assembly 300 is pressed downwards by external force, so that the liquid-absorbing head 306 enters the centrifuge tube 500 to be discharged, the external plunger pump performs liquid discharging action, and liquid in the liquid-absorbing head 306 is injected into the centrifuge tube 500 to be discharged, so that liquid transferring action is completed; the integrated zigzag liquid-transfering component 300 controls the liquid-transfering head 306 to repeatedly perform high-frequency liquid-transfering and liquid-transfering actions in a target centrifugal tube 500, so as to realize the uniform mixing operation of liquid; repeating the above steps many times, can accomplishing the nucleic acid extraction operation in the card box is inside, and finally, the sample after the purification carries out real-time fluorescence PCR amplification detection through integral type zigzag liquid-transfering component 300 partial shipment to corresponding centrifuging tube.
In conclusion, the closed card box can realize operations of liquid suction, liquid discharge, uniform mixing, liquid transfer and the like under the external drive, and finally realize full-automatic nucleic acid detection.
While the preferred embodiments of the present invention have been described above, the present invention is not limited to the specific details of the embodiments, and various equivalent changes may be made to the technical solution of the present invention within the technical spirit of the present invention, and these equivalent changes are within the protective scope of the present invention.
Claims (7)
1. The utility model provides a totally closed nucleic acid detection card box of rotatory liquid-transfering type which characterized in that: comprises a cylindrical hollow cavity and a disc-shaped base which is arranged at the bottom of the hollow cavity and is matched with the hollow cavity;
a cylindrical sleeve is arranged in the hollow cavity, penetrates through the hollow cavity and extends towards the two ends of the upper outer side and the lower outer side of the hollow cavity.
2. The rotary pipetting type all-closed nucleic acid detecting cartridge as recited in claim 1, wherein: the base comprises a base chassis, a base side wall arranged on the periphery of the base chassis and a gland arranged on the base;
a round hole is arranged in the center of the gland, and a plurality of gland circumferential holes with different sizes and diameters are arranged around the round hole;
a base center hole is formed in the center of the base, a raised thin-wall cylinder is arranged on the outer side of the base center hole, and a plurality of base circumferential holes with different sizes and diameters are further arranged around the base center hole;
a plurality of centrifugal tubes are arranged in the circumferential holes of the bases;
at least four convex buckles are arranged on the outer wall of the gland, and at least four buckle grooves matched with the convex buckles in position are formed in the side wall of the base.
3. The rotary pipetting type all-closed nucleic acid detecting cartridge as recited in claim 1, wherein: an integrated zigzag pipetting assembly matched with the cylindrical sleeve is arranged in the cylindrical sleeve,
the integral folded liquid-transferring assembly comprises a tower head at the top, a straight handle in the cylindrical shape at the middle part and a liquid-transferring assembly base provided with 4 fan-shaped hollows at the bottom, wherein a folded liquid-absorbing head adapter is arranged on the liquid-transferring assembly base, the folded liquid-absorbing head adapter comprises a conical boss, and a liquid-absorbing head is arranged on the conical boss;
an air passage is arranged in the integrated folded liquid-transferring assembly and is communicated with an external pipeline arranged on the top tower head and a liquid-absorbing head arranged on the conical boss;
a vertical guide rod is arranged at the lower part of the pipetting assembly base, penetrates between the round hole and the center hole of the base and comprises a pipetting assembly base, a guide rod body in a hollow structure and a guide rod base of a hollow cylinder at the lower part, wherein the upper part of the pipetting assembly base is connected with the guide rod body;
4 clamping grooves matched with the fan-shaped hollow-out liquid transfer assembly base are formed in the guide rod body; a plurality of fan-shaped grooves are arranged on the guide rod base; a spring is arranged inside the guide rod body;
the diameter of the inner wall of the guide rod base is matched with the outer diameter of the thin-wall cylinder protruding from the base, and a circle of groove is formed in the corresponding position.
4. The rotary pipetting type all-closed nucleic acid detecting cartridge as recited in claim 2, wherein: the gland is clamped in the base, and the diameter of the gland is smaller than that of the base; the position of the round hole is matched with the position of the central hole of the base, and the position of the circumferential hole of the gland is matched with the position of the circumferential hole of the base.
5. A rotary pipetting type all-closed nucleic acid detecting cartridge according to claim 1 or 3, wherein: the inner diameter of the cylindrical sleeve is matched with the outer diameter of a straight handle in the integrated zigzag liquid-transfering assembly, the outer diameter of the upper end of the straight handle is smaller than the inner diameter of the cylindrical sleeve, and the straight handle can slide up and down or rotate horizontally along the inner wall of the cylindrical sleeve;
the caliber of the top opening of the cylindrical sleeve is slightly smaller than the inner diameter of the cylinder body of the cylindrical sleeve.
6. The rotary pipetting type all-closed nucleic acid detecting cartridge as recited in claim 2, wherein: the diameter of the base chassis is slightly larger than the outer diameter of the side wall of the base, and the diameter of the base chassis and the outer diameter of the side wall of the base chassis are respectively matched with the outer diameter and the inner diameter of the hollow cavity;
a circle of thin-wall cylinder which protrudes outwards is arranged on the bottom surface of the base chassis around the circumferential hole of the base, and a circle of protrusion is arranged on the outer side of the cylinder.
7. The rotary pipetting type all-closed nucleic acid detecting cartridge as recited in claim 2, wherein: the centrifuging tube includes that 1.5mL volume and 200 mu L volume are two kinds, the centrifuging tube wear to establish in the base circumference hole, the aperture of base round hole slightly is less than the orificial bore of centrifuging tube, the fixed top that sets up at the centrifuging tube of gland.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011031726.3A CN112094729A (en) | 2020-09-27 | 2020-09-27 | Rotary liquid-transferring type totally-enclosed nucleic acid detection card box |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011031726.3A CN112094729A (en) | 2020-09-27 | 2020-09-27 | Rotary liquid-transferring type totally-enclosed nucleic acid detection card box |
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| CN112094729A true CN112094729A (en) | 2020-12-18 |
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| CN202011031726.3A Pending CN112094729A (en) | 2020-09-27 | 2020-09-27 | Rotary liquid-transferring type totally-enclosed nucleic acid detection card box |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112608813A (en) * | 2020-12-21 | 2021-04-06 | 上海思路迪生物医学科技有限公司 | Multi-degree-of-freedom library preparation card box with external power source and method |
| CN113150945A (en) * | 2021-04-14 | 2021-07-23 | 新羿制造科技（北京）有限公司 | Slide type cassette for nucleic acid extraction and PCR amplification detection |
| WO2023173606A1 (en) * | 2022-03-13 | 2023-09-21 | 首玺(广州)医疗科技有限责任公司 | Nucleic acid test kit and preparation process therefor |
-
2020
- 2020-09-27 CN CN202011031726.3A patent/CN112094729A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112608813A (en) * | 2020-12-21 | 2021-04-06 | 上海思路迪生物医学科技有限公司 | Multi-degree-of-freedom library preparation card box with external power source and method |
| CN112608813B (en) * | 2020-12-21 | 2022-07-01 | 上海思路迪生物医学科技有限公司 | Multi-degree-of-freedom library preparation card box with external power source and method |
| CN113150945A (en) * | 2021-04-14 | 2021-07-23 | 新羿制造科技（北京）有限公司 | Slide type cassette for nucleic acid extraction and PCR amplification detection |
| WO2023173606A1 (en) * | 2022-03-13 | 2023-09-21 | 首玺(广州)医疗科技有限责任公司 | Nucleic acid test kit and preparation process therefor |
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