CN218491744U - Sample detection pretreatment equipment and reagent card box therein - Google Patents
Sample detection pretreatment equipment and reagent card box therein Download PDFInfo
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- CN218491744U CN218491744U CN202221497556.2U CN202221497556U CN218491744U CN 218491744 U CN218491744 U CN 218491744U CN 202221497556 U CN202221497556 U CN 202221497556U CN 218491744 U CN218491744 U CN 218491744U
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Abstract
The invention relates to a detection reagent card box and equipment, belonging to the technical field of molecular diagnosis equipment. The detection reagent cartridge comprises: the liquid storage rotary disc is provided with a plurality of liquid grooves for containing liquid and sample tube holes for containing sample tubes; the liquid transferring device comprises a liquid transferring device for sucking and discharging liquid; the liquid storage rotary table is arranged in the box body, the inner space of the box body is divided into an upper cavity above the surface of the liquid storage rotary table and a lower cavity below the surface of the liquid storage rotary table, and the liquid storage rotary table can rotate and lift along the inner wall of the box body; the liquid transfer device is fixed on the box body, and the liquid storage rotary disc and the inner wall of the box body enable the upper cavity body to form a closed space; the box body is provided with a sample inlet communicated with the upper cavity and a card box cover matched with the sample inlet. The card box has the advantages of high detection speed and good sealing performance, can not generate cross infection, and solves the problem of pollution.
Description
Technical Field
The invention relates to the technical field of molecular diagnosis equipment, in particular to sample detection pretreatment equipment and a reagent card box therein.
Background
The detection process of the molecular diagnosis pathogen needs to carry out the treatment of the processes of cracking, washing, eluting, separating and purifying the nucleic acid of the pathogen, the purification steps of the pathogen are complicated, time-consuming and low in extraction efficiency, if the steps are carried out in an open environment, the pollution between samples such as aerosol and splash is easy to generate, or medical staff is infected by the pathogen, the purity of the extract is influenced, and the accuracy of the experimental result is further influenced.
For the above reasons, in the conventional technologies, there are also some researches on closed cartridges, such as the molecular diagnostic device technology, which have implemented the integration of multi-step pathogen detection methods into one system, the user only needs to add the sample into the system, and the rest steps are all automatically performed by the system.
Therefore, the card box and the micro-fluidic chip for molecular diagnosis adopt the closed and disposable detection card or detection tube, and cross and carry pollution are well controlled. However, these test cards or tubes often detect only one pathogen at a time.
In the conventional technology, some integrated pathogen detection cartridges can realize extraction, amplification and detection of nucleic acid to be detected in a closed system, so that pollution of a sample in the extraction and amplification processes is greatly avoided, and the accuracy and operability of nucleic acid detection are effectively improved.
Disclosure of Invention
Therefore, there is a need for a reagent cartridge with good sealing performance, which can detect multiple samples, is compatible with multiple reagents, is compatible with various reagents, does not need to package the reagents in the cartridge, has universality for different reagents, does not cause cross infection, and solves the problems of contamination of pathogenic microorganisms and infection of operators.
A detection reagent cartridge, comprising:
the liquid storage turntable is provided with a plurality of liquid grooves for containing liquid and sample tube holes for containing sample tubes, and the sample tube holes and a plurality of liquid groove samples are arranged around a rotating shaft of the liquid storage turntable;
the liquid transfer device comprises a liquid transfer device for sucking and discharging liquid, and the position of a liquid transfer head of the liquid transfer device corresponds to the position of the liquid groove; and
the liquid storage rotary table is arranged in the box body, the inner space of the box body is divided into an upper cavity above the surface of the liquid storage rotary table and a lower cavity below the surface of the liquid storage rotary table, and the liquid storage rotary table can rotate and lift along the inner wall of the box body; the liquid transfer device is fixed on the box body, the liquid transfer head is positioned in the upper cavity, and the liquid storage turntable and the inner wall of the box body form a closed space for the upper cavity; the box body is provided with a sample inlet communicated with the upper cavity and a card box cover matched with the sample inlet.
Above-mentioned detect reagent card box is equipped with the card lid, after accomplishing all application of sample through the sample entry, can cover the card box tightly, and later sample pretreatment process all accomplishes in the card box is inside, and the card box realizes inclosed, whole pollution-free. And above-mentioned detect reagent card box, the rotation of accessible stock solution carousel and the cooperation of sample entry need not to encapsulate reagent in advance inside the card box, and this card box of being convenient for adjusts and is used for different detection items.
In one embodiment, the liquid tank comprises a reagent tank, a reaction tank and a waste liquid tank. It can be understood that the number, functions, and the like of the various types of slots in the specific liquid tank can be set according to the requirements of specific detection items, for example, detection items with more waste liquid, a larger waste liquid tank, and the like can be designed.
In one embodiment, the liquid grooves and the sample tube holes are arranged in a circular ring shape around the axis of the liquid storage rotating disc, and the pipetting head and the sample inlet both correspond to the circular ring. According to the setting, can with pipettor and move liquid head is fixed, makes all liquid trench and sample tube hole homoenergetic correspond with fixed move liquid head through the rotation of stock solution carousel, has avoided need to remove the imbibition error or the operation degree of difficulty that the liquid head caused. The pipetting head in the conventional technology is usually separated from the pipetting equipment and connected with a hose, and the pipetting head has the risk of tube folding in the moving process, so that the pipetting precision is insufficient or the pipetting is not achieved.
In one embodiment, the pipette is a piston-type pipette. Through the immobility of liquid transfer head, and the pipettor is the design of liquid transfer piston integration formula for external drive is simpler, only need the drive piston in unilateral upward movement can, have the advantage that the operation is reliable, stable. It can be understood that the piston type pipettor can be selected from devices such as syringes, and the requirement of precision can be adjusted by controlling the size of the pipe diameter, namely the volume of the liquid volume which can be moved by moving the piston for the same distance.
In one embodiment, the number of the liquid transferring heads is two, the liquid storage turntable is further provided with a position avoiding hole, and when one liquid transferring head corresponds to the position of the liquid groove, the other liquid transferring head corresponds to the position of the position avoiding hole. It can be understood that, because the position of the liquid transfer head is unchanged when the liquid storage turntable moves upwards, if two liquid transfer heads are provided, one liquid transfer head is used for transferring liquid, and the other liquid transfer head is idle, if the idle liquid transfer head is not provided with a corresponding hole, the idle liquid transfer head can be abutted against the disc surface of the liquid storage turntable, and therefore the avoiding setting of the liquid transfer head needs to be considered. And the position of the position is provided with a position avoiding hole, so that the technical personnel in the field can set the position avoiding hole according to the specific detection project flow requirements and the number and the position of the applicable liquid transferring heads, and the position avoiding hole can be a specially designed hole position and can also be other reagent grooves and the like which do not influence the detection flow.
Meanwhile, the number of the liquid transfer heads is not limited, 1 liquid transfer head can operate to complete all processes, 2 liquid transfer heads can effectively avoid impurities of a sample from being brought into a pure sample, or more liquid transfer heads can complete all processes, but the number of the liquid transfer heads can influence the size of the liquid storage turntable and the number of the avoiding holes, so that the size of the whole cartridge is influenced, and the liquid transfer heads can be set according to the requirements of specific detection items.
In one embodiment, the inner wall of the box body is further provided with a limiting piece for limiting the liquid storage turntable to move downwards, and the limiting piece is arranged in the lower cavity. The limiting piece is used for preventing the liquid storage rotary table from falling off the box body after excessively moving downwards, and as can be understood, the specific shape and position of the limiting piece can be set according to the conventional technology in the field.
In one embodiment, a rotary disc sealing ring is arranged between the liquid storage rotary disc and the inner wall of the box body, and the liquid storage rotary disc and the box body form dynamic sealing. It can be understood that the sealing requirement of the upper cavity can be realized by other means in the field, but the dynamic sealing is realized by the rotary disc sealing ring, so that the rotary disc sealing device has the advantages of convenience, reliability and no influence on the movement of the liquid storage rotary disc.
In one embodiment, the detection reagent cartridge further comprises a guide bracket, the liquid storage rotary disc is arranged in the cartridge body through the guide bracket, and the rotary disc sealing ring is sleeved on the peripheral edge of the guide bracket. It can be understood that the guide of the liquid storage rotary disc is realized through the guide support and the liquid storage rotary disc is driven to move, and the liquid storage rotary disc has the advantages of stable movement and convenience in installation.
In one embodiment, the detection reagent cartridge further comprises a sealing cover for sealing the liquid storage rotary disc, the sealing cover is arranged on the guide bracket, and the liquid storage rotary disc and the sealing cover can rotate relatively. Through the application of sealed lid, can be after the processing of accomplishing the sample the stock solution carousel is sealed, realizes wholly removing, has further reduced the risk that produces the pollution. And the liquid storage rotary table and the sealing cover are both arranged on the guide support and can rotate relatively, so that the rotation of the liquid storage rotary table is not influenced.
In one embodiment, the detection reagent cartridge further comprises an elastic resetting piece, one end of the elastic resetting piece abuts against the sealing cover, the other end of the elastic resetting piece abuts against the inner wall of the cartridge body, and the elastic resetting piece is located in the upper cavity. The downward force can be indirectly applied to the liquid storage turntable through elastic reset pieces such as springs, and the purpose of downward moving and resetting the liquid storage turntable after liquid is taken by the liquid transfer device is achieved.
In one embodiment, the inner wall of the box body is further provided with a guide rod for guiding the guide support, the sealing cover is provided with a guide sleeve matched with the guide rod, and the guide rod and the guide sleeve are both arranged in the upper cavity. The guide rod can be sleeved outside the guide rod to achieve the guide effect.
In one embodiment, the sealing cover is mounted on the guide bracket and has a working state position and a sealing state position, when the sealing cover is in the working state position, the sealing cover and the liquid storage rotary disc can rotate relatively, when the sealing cover is in the sealing state position, the sealing cover is in snap fit with the liquid storage rotary disc and seals the liquid tank, the sealing cover is provided with at least two liquid taking holes, and the positions of the liquid taking holes correspond to the liquid moving head and the sample inlet respectively. It can be understood that when the sample is subjected to pretreatment pipetting and other steps in the cartridge, the pipetting head or an external mechanism can sample and sample through the liquid taking hole; after the sample is pretreated, the liquid storage turntable can be rotated to ensure that the liquid tank and the liquid taking hole are staggered and fixed in a snap fit manner, so that the purpose of sealing the liquid tank is achieved.
In one embodiment, the detection reagent cartridge further comprises a sample tube, wherein the sample tube comprises a tube cover and a tube body which are matched with each other in a clamping manner, the tube body is fixedly arranged in the sample tube hole through clamping connection, a sealing top cover is arranged on the sealing cover, and the tube cover is detachably connected with the sealing top cover through tight fit. The sample tube is used for containing samples which are finished through pretreatment, the samples can be transferred into the tube body after the pretreatment is finished through the clamping connection of the tube cover and the tube body, the tube cover is buckled on the tube body through the sealing top cover, and then the sample tube with the sealed buckle is transferred to other equipment to finish detection. The sample tube and the cartridge are separated at this time, and both are sealed after separation.
So the sample pipe is the PCR reaction pipe, and follow-up needs carry out the increase and detect the project, then the separation back of PCR reaction pipe, can realize on the qPCR instrument that increase and fluorescence detect, when real-time fluorescence quantitative PCR appearance (qPCR) detects a plurality of samples simultaneously (1 project of 1 sample corresponds 1 PCR reaction pipe), the volume is less. The defects that the PCR reaction tube is not separated from the card box, the qPCR instrument is directly used, the detection result is inaccurate, the occupied space is large and the detection of multiple samples is not facilitated are overcome.
Meanwhile, the number of the sample tubes is not limited, and the number of the sample tubes can be n, namely, joint detection of 1 sample corresponding to n detection items is realized.
In one embodiment, the outer wall of the pipe body is provided with a groove, a pipe body sealing ring is arranged in the groove, and the pipe body is in dynamic seal with the liquid storage rotary disc.
In one embodiment, the detection reagent cartridge further comprises a tube cover pushing mechanism, wherein the tube cover pushing mechanism is fixed on the inner wall of the cartridge body and protrudes towards the direction of the liquid storage rotating disc. The accessible push up go into the mechanism top and push away locate sealed capping on the lid makes the tube cap with the body buckle is fixed, realizes the sealed of tube cap and body.
The invention also discloses sample detection pretreatment equipment which comprises the detection reagent card box and a driving device, wherein the driving device comprises a lifting device and a rotating device, the rotating device drives the liquid storage rotary disc to rotate along a rotating shaft of the liquid storage rotary disc, and the lifting device drives the liquid storage rotary disc to lift.
The sample detection pretreatment equipment drives the liquid storage turntable to lift and rotate by the lifting device and the rotating device so as to finish pipetting and promote a sample to carry out pretreatment reaction.
In one embodiment, the lifting device comprises a push rod and a push rod driver, one end of the push rod is mounted on the push rod driver, the other end of the push rod abuts against the liquid storage rotary table, and the push rod driver drives the push rod to contract or extend to drive the liquid storage rotary table to move up and down; the rotating device comprises a central shaft of the liquid storage turntable and a driving device for driving the central shaft to rotate. For example, the central shaft can be clamped and rotated by an external device to drive the liquid storage turntable to do circular motion.
In one embodiment, the sample pretreatment device further comprises a pipetting driving device, a piston rod and an elastic piston are arranged in the pipette, a rod hole tightly matched with the piston rod is formed in the elastic piston, and the pipetting driving device is a grabbing device used for driving the piston rod to move.
In one embodiment, the sample pretreatment equipment further comprises an incubation oscillating device, a magnetic attraction device and a moving mechanism, wherein the liquid tank comprises a reagent tank, a reaction tank and a waste liquid tank, and the incubation oscillating device and the magnetic attraction device can be driven by the moving mechanism to be close to or far away from the reaction tank. It can be understood that, if the sample detection is molecular diagnosis detection, the pretreatment apparatus includes an incubation oscillation device and a magnetic attraction device for nucleic acid extraction and purification, and the movement of the devices is realized by using a conventional moving mechanism, and when the incubation oscillation or magnetic attraction is required, the corresponding device is close to or covers a corresponding liquid tank (such as a reaction tank) to complete the pretreatment.
In one embodiment, the sample pre-detection processing apparatus further comprises a mechanical gripper for gripping the sample tube. It can be understood that the mechanical gripper is a conventional mechanical element and is usually arranged in the field, but the mechanical gripper is matched with the sample tube, so that the pretreatment equipment not only realizes full-automatic operation, but also has the advantages of small occupied space and high equipment flexibility.
The invention also discloses a sample detection pretreatment method, which adopts the pretreatment equipment and comprises the following steps:
sample adding: opening the cassette cover, controlling the rotating device to drive the liquid storage turntable to rotate until the liquid groove is aligned with the sample inlet, adding a sample to be processed and a reagent, and tightly covering the cassette cover;
reaction: controlling the rotating device to drive the liquid storage rotary table to rotate, enabling the position of the liquid transfer head to correspond to the position of a required liquid groove, controlling the lifting device to drive the liquid storage rotary table to ascend, enabling the liquid transfer head to be located in the corresponding liquid groove, controlling the liquid transfer device to absorb liquid, controlling the lifting device to enable the liquid storage rotary table to descend, controlling the liquid storage rotary table to rotate to a preset position, completing liquid transfer, and enabling a sample to complete a pretreatment reaction in the liquid groove;
sampling: and controlling the pipetting device to suck the pretreated sample solution out, transferring the sample solution into a sample tube, and taking out the sample tube.
Compared with the prior art, the invention has the following beneficial effects:
the detection reagent card box is provided with the card box cover, the card box cover can be tightly covered after all sample adding is finished through the sample inlet, the subsequent sample pretreatment process is finished in the card box, the card box is sealed, and no pollution is caused in the whole process. And above-mentioned detect reagent card box, the rotation of accessible stock solution carousel and the cooperation of sample entry need not to encapsulate reagent in advance inside the card box, and this card box of being convenient for adjusts and is used for different detection items.
Through the mode that adopts the stock solution carousel rotation, the axis that makes the liquid groove aligns with the axis of moving the liquid head, and liquid groove etc. all distribute in this card box with moving the liquid head in same rotation center, for the mode that the liquid head was moved in the translation, perhaps the mode of translation stock solution dish, space is more economized to the rotatory mode, and the translation has the parking space that produces after the removal, and the rotation need not unnecessary parking space, can design the card box volume littleer, more practices thrift the space.
And the card box is processed, manufactured and assembled, high-difficulty element equipment is not involved, and the card box has the advantages of low manufacturing and assembling cost and autonomous controllable yield.
Drawings
FIG. 1 is a first schematic view of a first configuration of a test reagent cartridge;
FIG. 2 is a schematic view of a second embodiment of the detection reagent cartridge;
FIG. 3 is an exploded view of a portion of the structure of the detector reagent cartridge;
FIG. 4 is a schematic view of the external configuration of the test reagent cartridge;
FIG. 5 is a schematic view of a liquid storage turntable;
FIG. 6 is a schematic view of the structure of the pipetting device and the guide bar;
FIG. 7 is a schematic view of a sample tube structure;
FIG. 8 is a top view of the liquid storage disk and the sample tube;
FIG. 9 is a sectional view of EE of FIG. 8;
FIG. 10 is a top view of the seal cap;
FIG. 11 is a cross-sectional view of the sealing cover DD;
FIG. 12 is a schematic view showing the direction of movement of a liquid storage turntable in the detection reagent cartridge.
Wherein: 100. a liquid storage rotary table; 110. a reaction tank; 120. a waste liquid tank; 131. a first reagent tank; 132. a second reagent tank; 133. a third reagent tank; 134. a fourth reagent tank; 141. a first avoiding hole; 142. a second avoiding hole; 143. a third avoiding hole; 144. a fourth avoiding hole; 145. a fifth avoiding hole; 150. a sample tube aperture; 200. a pipetting device; 211. a number 1 pipetting head; 212. a No. 2 pipetting head; 300. a box body; 310. a sample inlet; 320. a card box cover; 330. a jacking mechanism; 400. a sample tube; 410. a tube cover; 420. a pipe body; 421. a pipe body sealing ring; 500. a guide bracket; 510. a rotary disc sealing ring; 520. an elastic reset member; 530. a stopper; 540. a guide rod; 600. a sealing cover; 610. a liquid extraction hole; 620. a guide sleeve; 630. and sealing the top cover.
Detailed Description
To facilitate an understanding of the invention, the invention will now be described more fully hereinafter with reference to the accompanying drawings. Preferred embodiments of the present invention are shown in the drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "mounted" to another element, it can be directly mounted to the other element or intervening elements may also be present.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
Example 1
A test reagent cartridge, as shown in fig. 1-4, comprising: a liquid storage rotary disc 100, a pipetting device 200 and a box body 300. And, if necessary, a special sample tube 400 may be provided or not, and in this embodiment, the sample tube 400 is provided as an example.
The liquid storage turntable 100 is provided with a plurality of liquid tanks for containing liquid and sample pipe holes 150 for containing sample pipes 400, and the sample pipe holes 150 and the plurality of liquid tank samples are arranged around the rotating shaft of the liquid storage turntable 100. Specifically, the liquid grooves and the sample tube holes 150 are arranged in a circular ring shape around the axis of the liquid storage turntable 100, and the pipetting head and the sample inlet 310 correspond to the circular ring position respectively.
In this embodiment, the liquid tanks include reagent tanks (first reagent tank 131, second reagent tank 132, third reagent tank 133, fourth reagent tank 134), reaction tank 110, and waste liquid tank 120. It can be understood that the number and the effect of various types of slots in a specific liquid tank can be set according to the requirements of specific detection items, for example, detection items with more waste liquid are generated, and a larger waste liquid tank can be designed, so that liquid leakage or pollution caused by operations such as oscillation on a sample in the subsequent treatment process can be avoided. In this embodiment, the detection reagent cartridge further comprises a guiding bracket 500, wherein the liquid storage turntable 100 is installed in the cartridge body 300 through the guiding bracket 500, and the turntable sealing ring 510 is sleeved on the peripheral edge of the guiding bracket 500, so that the guiding bracket 500 drives the liquid storage turntable 100 to form dynamic seal with the cartridge body 300.
For the downward movement limiting of the liquid storage turntable 100, the inner wall of the box body 300 is further provided with a limiting piece 530 for limiting the downward movement of the liquid storage turntable 100, and the limiting piece 530 is arranged in the lower cavity. The limiting member 530 is used to prevent the liquid storage turntable 100 from falling off the cartridge 300 after moving down excessively, and it can be understood that the specific shape and position of the limiting member 530 may be set according to the conventional technology in the art, in this embodiment, the limiting member 530 is a limiting plate installed around the inner wall of the cartridge.
The pipetting device 200 includes a piston type pipettor (e.g., a piston type pipetting member similar to a syringe structure) for aspirating and discharging liquid, and the pipetting head position of the pipettor corresponds to the liquid slot position. It can be understood that the piston type liquid transfer device can be selected from devices such as a syringe, and the requirement on precision can be adjusted by controlling the size of the pipe diameter, namely the volume of the liquid which can be moved by moving the piston for the same distance.
In this embodiment, there are two liquid-transferring heads, and the liquid-storing turntable 100 is further provided with a position-avoiding hole, and when one of the liquid-transferring heads corresponds to the liquid tank, the other liquid-transferring head corresponds to the position-avoiding hole. It can be understood that, since the positions of the pipetting heads are not changed when the liquid storage turntable 100 moves upwards, if two pipetting heads are used, when one pipetting head is used for pipetting, and the other pipetting head is idle, if the idle pipetting head is not placed with a corresponding hole, the idle pipetting head will abut against the disk surface of the liquid storage turntable 100, and therefore the avoiding arrangement of the pipetting heads needs to be considered. And the position of the position is provided with a position avoiding hole, so that the technical personnel in the field can set the position avoiding hole according to the specific detection project flow requirements and the number and the position of the applicable liquid transferring heads, and the position avoiding hole can be a specially designed hole position and can also be other reagent grooves and the like which do not influence the detection flow.
According to the requirement of molecular diagnosis as the detection item in this embodiment and the design of the two pipetting heads, as shown in fig. 5-6, the holes on the rotating disk 100 are arranged to receive the sample solution to be processed and the reaction tank 110 for reaction, and the reaction tank 110, the fourth avoiding hole 144, the waste liquid tank 120, the third avoiding hole 143, the fourth reagent tank 134, the second reagent tank 132, the third reagent tank 133, the first avoiding hole 141, the second avoiding hole 142, the sample tube hole 150 and the fifth avoiding hole 145 are sequentially arranged along the circumferential direction of the rotating disk 100.
The liquid storage rotary disc 100 is installed in the cartridge 300 through a guide bracket 500, and the internal space of the cartridge 300 is divided into an upper cavity above the disc surface of the liquid storage rotary disc 100 and a lower cavity below the disc surface of the liquid storage rotary disc 100, and the liquid storage rotary disc 100 can rotate and move up and down along the inner wall of the cartridge 300; the pipetting device 200 is fixed on the box body 300, the pipetting head is positioned in the upper cavity, and the liquid storage turntable 100 and the inner wall of the box body 300 form a closed space in the upper cavity; the cartridge body 300 is provided with a sample inlet 310 communicating with the upper chamber and a cartridge cover 320 matching the sample inlet 310.
In order to further reduce the risk of contamination, the cartridge of this embodiment further includes a sealing cover 600 for sealing the liquid storage turntable 100, the sealing cover 600 is mounted on the guide bracket 500, and the liquid storage turntable 100 is rotatable relative to the sealing cover 600. Through the application of sealed lid, can be after the processing of accomplishing the sample the stock solution carousel is sealed, realizes wholly removing, has further reduced the risk that produces the pollution. And the liquid storage rotary table and the sealing cover are both arranged on the guide support and can rotate relatively, so that the rotation of the liquid storage rotary table is not influenced.
In this embodiment, the detection reagent cartridge further comprises an elastic reset member 520, wherein one end of the elastic reset member 520 abuts against the sealing cover 600, and the other end abuts against the inner wall of the cartridge body. The liquid storage turntable 100 can be indirectly exerted with downward force through an elastic reset piece 520 such as a spring, and the purpose of downward movement and reset of the liquid storage turntable after liquid is taken by the liquid transfer device is achieved.
The inner wall still is equipped with downwardly extending's guide bar 540 that is used for the direction of guide bracket 500 on the box body, sealed lid 600 is equipped with guide sleeve 620 of guide bar 540 complex, guide bar 540 and guide sleeve 620 all locate in the upper chamber body. The guide function can be realized by sleeving the guide rod outside the guide rod. And the elastic restoring member 520 (spring) is also sleeved outside the guide sleeve 620.
Specifically, the sealing cover 600 is mounted on the guide bracket 500 and has a working state position and a sealing state position, when the sealing cover 600 is in the working state position, the sealing cover 600 and the liquid storage rotary disc 100 can rotate relatively, when the sealing cover 600 is in the sealing state position, the sealing cover 600 is in snap fit with the liquid storage rotary disc 100 and seals the liquid tank, the sealing cover 600 is provided with at least two liquid taking holes 610, and the positions of the liquid taking holes 610 correspond to the liquid moving head and the sample inlet 310 respectively. It is understood that when the sample is pre-processed in the cartridge for pipetting, the pipetting head or external mechanism can sample and sample through the pipetting holes 610; after the pretreatment of the sample is completed, the liquid storage turntable 100 can be rotated to make the liquid tank and the liquid taking hole 610 dislocated and fixed in a snap fit manner, so that the purpose of sealing the liquid tank is achieved.
The sample tube 400 in this embodiment includes a tube cap 410 and a tube body 420 that are in snap fit, as shown in fig. 7-11, the tube body 420 is fixed in the sample tube hole 150 through snap fit connection, the sealing cap 600 is provided with a sealing top cap 630, and the tube cap 410 is detachably connected to the sealing top cap 630 through tight fit.
Specifically, a groove is formed in the outer wall of the pipe body 420, a sealing ring of the pipe body 420 is arranged in the groove, and the pipe body 420 and the liquid storage turntable 100 are in dynamic sealing.
In order to fasten the tube cap 410 to the tube 420, the detection reagent cartridge further includes a tube cap 410 pushing mechanism 330, wherein the tube cap 410 pushing mechanism 330 is fixed on the inner wall of the case 300 and protrudes toward the liquid storage turntable 100. The sealing top cover 630 arranged on the sealing cover 600 can be pushed by the pushing mechanism 330, the pipe cover 410 is extruded to be fastened and fixed with the pipe body 420, the sealing of the pipe cover 410 and the pipe body 420 is realized, and further, the full-automatic operation is realized.
Example 2
A sample pretreatment device for molecular diagnosis, which employs the detection reagent cartridge of embodiment 1 and a driving device, wherein the driving device includes a lifting device and a rotating device, the rotating device drives the liquid storage turntable 100 to rotate along its rotating shaft, and the lifting device drives the liquid storage turntable 100 to lift, as shown in fig. 12.
The sample detection pretreatment equipment drives the liquid storage turntable 100 to realize lifting and rotating by the lifting device and the rotating device so as to finish pipetting and promote a sample to carry out pretreatment reaction.
Specifically, the lifting device comprises a push rod and a push rod driver, one end of the push rod is mounted on the push rod driver, the other end of the push rod abuts against the liquid storage turntable 100, and the push rod is driven by the push rod driver to contract or extend to drive the liquid storage turntable 100 to move up and down; the rotating device comprises a central shaft of the liquid storage turntable 100 and a driving device for driving the central shaft to rotate. For example, the central shaft can be clamped and rotated by an external device to drive the liquid storage turntable 100 to make a circular motion.
In order to realize the automatic operation process, the sample detection pretreatment equipment further comprises a liquid transfer driving device, a piston rod and an elastic piston are arranged in the liquid transfer device, a rod hole in close fit with the piston rod is formed in the elastic piston, and the liquid transfer driving device is a grabbing device for driving the piston rod to move.
For example, the piston rod aligns with No. 1 pipettor the central axis in the cassette, and is downward along the axis, reaches the piston to the bottommost, and the top of piston rod can be strained to the downthehole on piston (piston is elastic material) surface at this moment, realizes piston rod and piston integration, and the drive drives the piston rod up-and-down motion, realizes the pipetting of No. 1 pipettor. The grabbing device also grabs the piston rod of the No. 2 pipettor, so that the piston rod and the piston are integrated, the grabbing device is driven to move up and down, and the pipetting of the No. 2 pipetting head 212 is realized.
Because the pretreatment equipment of this embodiment is used for molecular diagnostic procedures, and this sample detection pretreatment equipment needs to carry out nucleic acid extraction and purification to the sample, if adopt the magnetic bead to extract and purify, therefore, this equipment still includes to incubate shaking device, magnetism device and moving mechanism, incubate shaking device and magnetism device all can be close to or keep away from reaction tank 110 under the drive of moving mechanism. It can be understood that, if the sample detection is molecular diagnosis detection, the pretreatment apparatus includes an incubation oscillation device and a magnetic attraction device for nucleic acid extraction and purification, and the movement of the devices is realized by using a conventional moving mechanism, so that when the incubation oscillation or magnetic attraction is required, the corresponding device is close to or covers the corresponding liquid tank (e.g. the reaction tank 110) to complete the pretreatment.
Further, the sample pre-detection processing apparatus further comprises a mechanical gripper for gripping the sample tube 400. It can be understood that the mechanical gripper is a conventional mechanical element, and is usually arranged in the field, but the mechanical gripper is matched with the sample tube 400, so that the pretreatment equipment not only realizes full-automatic operation, but also has the advantages of small occupied space and high equipment flexibility.
Example 3
A pretreatment method for a molecular diagnostic test sample, which adopts the pretreatment apparatus of the embodiment 2, comprising the steps of:
1. and (4) sample adding.
And opening the cassette cover 320, controlling the rotating device to drive the liquid storage turntable 100 to rotate until the liquid groove is aligned with the sample inlet 310, adding the sample to be processed and the reagent, and tightly covering the cassette cover 320. The embodiment is as follows:
1. the external mechanism drives the cartridge cover 320 to unscrew;
2. at the moment, the reaction tank 110 on the liquid storage turntable 100 is aligned with the axis of the sample inlet 310, and 300uL of RNA capture solution, 20uL of proteinase K and 15uL of magnetic bead suspension are sequentially added into a pipette gun of an automatic instrument outside the cartridge;
3. rotating the carousel 100 with the second reagent reservoir 132 aligned with the sample inlet 310 axis, and adding 500uL of the rinse PWC at a time to the automated instrument pipette outside the cartridge;
4. rotating the liquid storage turntable 100, wherein the third reagent groove 133 is aligned with the axis of the sample inlet 310, and adding 1000uL of rinsing liquid PWE at a time to the liquid transfer gun of the automated instrument outside the cartridge;
5. the reservoir carousel 100 is rotated, with the fourth reagent well 134 aligned with the sample inlet 310 axis, and the automated instrument pipette outside the cartridge adds 200uL of RNase-free double distilled water at a time.
6. Rotating the reservoir carousel 100, with the reaction well 110 aligned with the axis of the sample inlet 310, and adding 200uL of sample by an external mechanism (this sample addition process needs to be performed in a closed environment containing high efficiency filtration);
7. the external mechanism screws on the cartridge cover 320 and the entire cartridge is completely sealed.
2. And (4) pretreatment reaction.
Controlling the rotating device to drive the liquid storage turntable 100 to rotate, enabling the pipetting head to correspond to the position of a required liquid groove, controlling the lifting device to drive the liquid storage turntable 100 to ascend, enabling the pipetting head to be located in the corresponding liquid groove, controlling the pipetting device 200 to aspirate liquid, controlling the lifting device to enable the liquid storage turntable 100 to descend, controlling the liquid storage turntable 100 to rotate to a preset position, as shown in fig. 11, completing pipetting, and enabling a sample to complete a pretreatment reaction in the liquid groove. The details in this embodiment are as follows:
1. after the sample is added to the reaction tank 110, the reaction tank 110 contains: sample +300uL RNA capture solution +20uL proteinase K +15uL magnetic bead suspension;
2. adding an incubation and oscillation device at the position of the reaction tank 110, incubating and oscillating the liquid in the reaction tank 110 to complete the cracking of the sample, releasing the nucleic acid, and oscillating the nucleic acid to make the nucleic acid adsorbed on the magnetic beads;
3. a magnetic ring is added at the position of the reaction tank 110, so that the magnetic beads adsorbed with the nucleic acid are adsorbed on the side wall of the reaction tank 110; 4. rotating the liquid storage turntable 100 to align the axis of the No. 1 pipetting head 211 with the reaction tank 110, then pushing the liquid storage turntable 100 integrally upward, pushing the needle tip of the No. 1 pipetting head 211 to leave only a tiny gap position with the bottom of the slot position of the reaction tank 110, driving the piston of the No. 1 pipetting head 211 upward, and sucking waste liquid of the reaction tank 110 except nucleic acid adsorbed on the side wall; (at this time, the No. 2 liquid transferring head 212 is at the position of the fourth avoiding hole 144);
5. the push rod is driven to contract, the liquid storage rotary table 100 is pushed to integrally move downwards by abutting against a spring of the guide support 500 until the liquid storage rotary table 100 reaches the limiting piece 530 of the liquid storage rotary table 100, and the downward resetting of the liquid storage rotary table 100 is completed;
6. rotating the liquid storage rotary disc 100 to align the waste liquid tank 120 with the axis of the No. 1 liquid transfer head 211, then pushing the liquid storage rotary disc 100 integrally to the upper part, pushing the needle point of the No. 1 liquid transfer head 211 and the position of the hole site bottom of the waste liquid tank 120 with a gap, driving the piston of the No. 1 liquid transfer head 211 downwards, and discharging the waste liquid in the No. 1 liquid transfer head 211 to the waste liquid tank 120; the push rod is driven to contract, the liquid storage rotary table 100 is pushed to integrally move downwards by abutting against a spring of the guide support 500 until the liquid storage rotary table 100 reaches the limiting piece 530 of the liquid storage rotary table 100, and the downward resetting of the liquid storage rotary table 100 is completed; (in this case, the No. 2 pipetting head 212 is at the No. 4 well position, and the No. 4 well is filled with double distilled water, so that the cleanliness of the No. 2 pipetting head 212 is not affected)
7. Similarly to the steps 4 and 5, the liquid storage turntable 100 is rotated to align the second reagent tank 132 with the axis of the No. 1 pipetting head 211, and 500uL of the rinsing liquid PWC is aspirated; (in this case, the No. 2 pipetting head 212 is in the first avoiding hole 141)
8. Similarly to step 6, the liquid storage turntable 100 is rotated to align the reaction tank 110 with the axis of the No. 1 pipetting head 211, and the PWC in the No. 1 pipetting head 211 is discharged to the reaction tank 110; (the No. 2 liquid transfer head 212 is at the fourth avoiding hole 144 at this time); after the oscillating incubation and the magnetic attraction are driven, waste liquid is sucked, the waste liquid tank 120 is aligned with the axis of the No. 1 liquid-moving head 211, and the waste liquid in the No. 1 liquid-moving head 211 is discharged to the waste liquid tank 120; (at this time, the No. 2 pipetting head 212 is at the position of the fourth reagent tank 134, and double distilled water is filled in the fourth reagent tank 134, so that the cleanliness of the No. 2 pipetting head 212 is not influenced); the push rod is driven to contract, the liquid storage rotary disc 100 is pushed to integrally move downwards by abutting against a spring of the guide support 500 until the liquid storage rotary disc 100 reaches the limiting piece 530 of the liquid storage rotary disc 100, and the desired downward resetting of the liquid storage rotary disc 100 is completed;
9. similar to the step 7, the liquid storage turntable 100 is rotated to align the hole position # 3 with the axis of the No. 1 pipetting head 211, and 500uL of rinsing liquid PWE is sucked; (the No. 2 liquid transfer head 212 is at the second avoiding hole 142 at this time);
10. repeating the step 8; the PWE after rinsing is transferred to a waste liquid tank 120;
11. repeating the step 9 and the step 10;
12. similar to the step 7, the liquid storage turntable 100 is rotated to align the fourth reagent tank 134 with the axis of the No. 1 pipetting head 211, and 100uL of double distilled water without RNase is sucked to the No. 1 reaction hole; (at this time, the No. 2 pipetting head 212 is in the third pipetting groove, and the cleanliness of the No. 2 pipetting head 212 cannot be affected because the third pipetting groove is empty after being sucked in the steps 9, 10 and 11);
13. repeating the step 8; transferring the RNase-free double distilled water to a waste liquid tank 120;
14. similar to the step 7, the liquid storage rotary disc 100 is rotated to align the fourth reagent groove 134 with the axis of the No. 1 pipetting head 211, and 100uL of double distilled water without RNase is sucked to the 110 th position of the reaction groove; nucleic acid is dissolved in double distilled water, vibration and uniform mixing are driven, and the liquid storage rotating disc 100 is reset.
15. Adding a magnetic ring at the position of the reaction tank 110 to make the magnetic beads be adsorbed on the side wall of the reaction tank 110;
16. similar to step 7, the liquid storage turntable 100 is rotated to align the reaction tank 110 with the axis of the No. 2 pipetting head 212, and 5uL of purified nucleic acid is pipetted (kept in a magnetic attraction state); (in this case, the No. 1 liquid transfer head 211 is arranged at the fifth avoiding hole 145)
17. Similarly to step 8, the liquid storage turntable 100 is rotated to align the sample tube hole 150 with the axis of the No. 2 pipette head 212, and the nucleic acid in the No. 2 pipette head 212 is discharged to the sample tube 400 in the sample tube hole 150; (the No. 1 pipetting head 211 is at the avoiding position 2 holes at the moment)
18. The transfer of the purified nucleic acid to the sample tube 400 (the reaction tube contains the PCR reaction solution) is completed.
3. And (6) sampling.
The pipetting device 200 is controlled to aspirate the pretreated sample solution, transfer the aspirated sample solution into the sample tube 400, and take out the sample tube 400, as shown in fig. 12. The details are as follows:
1. rotating the liquid storage rotary disc 100 to align the 150 positions of the sample tube holes with the axis of the jacking mechanism 330, pushing the whole liquid storage rotary disc 100 upwards, controlling a mechanical gripper to grip the tube body 420 (PCR reaction tube) of the sample tube 400 while pushing, pushing upwards along with the liquid storage rotary disc 100 in a step-by-step manner, pushing the liquid storage rotary disc 100 to be covered on the liquid storage rotary disc 100 by the sealing cover 600, and then enabling the height position of the liquid storage rotary disc 100 in the vertical direction to be higher than the position of the liquid absorption; continuously pushing upwards to push the tube cap 410 to cover the tube body 420 (i.e. the PCR reaction tube) to complete the sealing of the PCR tube; the liquid storage rotary disc 100 is continuously pushed upwards, but the mechanical hand for gripping the sample tube 400 does not move upwards along with the liquid storage rotary disc 100, at the moment, the sample tube 400 is covered with the cover, the sample tube 400 is separated from the card box body, the inside of the card box is still of a closed structure, the PCR tube is also of a fully closed structure, and the PCR tube and the card box are sealed.
2. The externally driven grabbing card box body is discarded;
3. the sample tube 400 (PCR reaction tube) is gripped by a mechanical gripper to a qPCR instrument for amplification detection, so that PCR extraction, amplification and detection are completed.
The pretreatment process of the molecular diagnosis detection sample is a fully-sealed treatment process, the sealing is realized by two steps, and the whole liquid transfer process of nucleic acid extraction and system construction is completed in the card box to realize sealing; 2. after the PCR reaction tube is separated from the card box, the inside of the card box is still in a closed structure, and the PCR reaction tube is also in a fully closed structure to realize sealing.
All possible combinations of the technical features of the above embodiments may not be described for the sake of brevity, but should be considered as within the scope of the present disclosure as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (20)
1. A test reagent cartridge, comprising:
the liquid storage turntable is provided with a plurality of liquid grooves for containing liquid and sample tube holes for containing sample tubes, and the sample tube holes and the plurality of liquid grooves are arranged around a rotating shaft of the liquid storage turntable;
the liquid transfer device comprises a liquid transfer device for sucking and discharging liquid, and the position of a liquid transfer head of the liquid transfer device corresponds to the position of the liquid groove; and
the liquid storage rotary table is arranged in the box body, the inner space of the box body is divided into an upper cavity above the surface of the liquid storage rotary table and a lower cavity below the surface of the liquid storage rotary table, and the liquid storage rotary table can rotate and lift along the inner wall of the box body; the liquid transfer device is fixed on the box body, the liquid transfer head is positioned in the upper cavity, and the liquid storage turntable and the inner wall of the box body form a closed space for the upper cavity; the box body is provided with a sample inlet communicated with the upper cavity and a card box cover matched with the sample inlet.
2. The assay reagent cartridge of claim 1, wherein the liquid reservoir comprises a reagent reservoir, a reaction reservoir, a waste reservoir.
3. The assay reagent cartridge of claim 1, wherein the plurality of fluid wells and the sample tube apertures are arranged in a circular ring around the axis of the reservoir carousel, and the pipetting head and the sample inlet both correspond to the circular ring.
4. A test reagent cartridge according to claim 1, wherein the pipette is a piston-type pipette.
5. The detection reagent cartridge of claim 4, wherein the number of the pipetting heads is two, the liquid storage turntable is further provided with a position avoiding hole, and when one pipetting head corresponds to the position of the liquid groove, the other pipetting head corresponds to the position avoiding hole.
6. The detection reagent cartridge of claim 1, wherein the inner wall of the cartridge body is further provided with a limiting member for limiting the downward movement of the liquid storage turntable, and the limiting member is disposed in the lower chamber.
7. The assay reagent cartridge of claim 1, wherein a rotary seal is disposed between the reservoir rotary disk and the inner wall of the cartridge body, and the reservoir rotary disk forms a dynamic seal with the cartridge body.
8. The assay reagent cartridge of claim 7, further comprising a guide bracket, wherein the reservoir carousel is mounted within the cartridge body via the guide bracket, and the carousel sealing ring is disposed around a peripheral edge of the guide bracket.
9. The assay reagent cartridge of claim 8, further comprising a sealing cap for sealing the reservoir disk, the sealing cap being mounted on the guide support, the reservoir disk being rotatable relative to the sealing cap.
10. The detector reagent cartridge of claim 9 further comprising an elastic reset member, wherein one end of the elastic reset member abuts against the sealing cover, the other end of the elastic reset member abuts against the inner wall of the cartridge body, and the elastic reset member is located in the upper chamber.
11. The detection reagent cartridge of claim 10, wherein the inner wall of the cartridge body is further provided with a guide rod for guiding the guide bracket, the sealing cover is provided with a guide sleeve matched with the guide rod, and the guide rod and the guide sleeve are both arranged in the upper chamber.
12. The test reagent cartridge of claim 9, wherein the sealing cover is mounted on the guide frame and has an operating position and a sealing position, wherein the sealing cover is rotatable relative to the reservoir turntable when the sealing cover is in the operating position, wherein the sealing cover is snap-fitted to the reservoir turntable and seals the liquid tank when the sealing cover is in the sealing position, and wherein the sealing cover has at least two liquid dispensing holes at positions corresponding to the liquid dispensing head and the sample inlet, respectively.
13. The assay reagent cartridge of claim 9, further comprising a sample tube, wherein the sample tube comprises a tube cover and a tube body which are in snap fit, the tube body is fixed in the sample tube hole through snap fit connection, the sealing cover is provided with a sealing top cover, and the tube cover is detachably connected with the sealing top cover through tight fit.
14. The test reagent cartridge of claim 13, wherein the outer wall of the tube has a groove, and a tube seal is disposed in the groove and the tube is in fluid-tight contact with the reservoir disk.
15. The detector reagent cartridge of claim 13 further comprising a cap insertion mechanism fixed to the inner wall of the cartridge body and protruding toward the reservoir disk.
16. A sample pretreatment apparatus, comprising the detection reagent cartridge according to any one of claims 1 to 15, and a driving device, wherein the driving device comprises a lifting device and a rotating device, the rotating device drives the liquid storage disk to rotate along a rotating shaft thereof, and the lifting device drives the liquid storage disk to lift.
17. The pretreatment equipment for sample detection according to claim 16, wherein the lifting device comprises a push rod and a push rod driver, one end of the push rod is mounted on the push rod driver, the other end of the push rod abuts against the liquid storage turntable, and the push rod is driven by the push rod driver to contract or extend to drive the liquid storage turntable to move up and down; the rotating device comprises a central shaft of the liquid storage turntable and a driving device for driving the central shaft to rotate.
18. The pretreatment equipment for sample detection according to claim 16, further comprising a liquid transfer driving device, wherein the liquid transfer device is provided with a piston rod and an elastic piston, the elastic piston is provided with a rod hole tightly matched with the piston rod, and the liquid transfer driving device is a gripping device for driving the piston rod to move.
19. The pretreatment apparatus before sample detection according to claim 16, further comprising an incubation oscillating device, a magnetic attracting device, and a moving mechanism, wherein the liquid tank comprises a reagent tank, a reaction tank, and a waste liquid tank, and both the incubation oscillating device and the magnetic attracting device can be driven by the moving mechanism to move close to or away from the reaction tank.
20. The pre-test treatment apparatus of claim 16, further comprising a mechanical gripper for gripping the sample tube.
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| CN202221497556.2U CN218491744U (en) | 2022-06-15 | 2022-06-15 | Sample detection pretreatment equipment and reagent card box therein |
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| CN202221497556.2U CN218491744U (en) | 2022-06-15 | 2022-06-15 | Sample detection pretreatment equipment and reagent card box therein |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117778172A (en) * | 2023-12-28 | 2024-03-29 | 江苏省淡水水产研究所 | Shrimp iridovirus PCR detection device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117778172A (en) * | 2023-12-28 | 2024-03-29 | 江苏省淡水水产研究所 | Shrimp iridovirus PCR detection device |
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Effective date of registration: 20230926 Address after: Room 301, G10, South China new material innovation park, building 3, No. 31, Kefeng Road, Guangzhou hi tech Industrial Development Zone, Guangdong 510130 Patentee after: Guangzhou Weiyuan Medical Equipment Co.,Ltd. Patentee after: GUANGZHOU VISION GENE TECHNOLOGY Co.,Ltd. Patentee after: Guangzhou Weiyuan medical laboratory Co.,Ltd. Patentee after: Shenzhen Weiyuan Medical Technology Co.,Ltd. Patentee after: Beijing Weiyuan Medical Laboratory Co.,Ltd. Address before: Room 301, G10, South China new material innovation park, building 3, No. 31, Kefeng Road, Guangzhou hi tech Industrial Development Zone, Guangdong 510130 Patentee before: Guangzhou Weiyuan Medical Equipment Co.,Ltd. Patentee before: GUANGZHOU VISION GENE TECHNOLOGY Co.,Ltd. Patentee before: Guangzhou Weiyuan medical laboratory Co.,Ltd. Patentee before: Shenzhen Weiyuan Medical Technology Co.,Ltd. Patentee before: Weiyuan (Shenzhen) Medical Research Center Co.,Ltd. Patentee before: Nanjing Weiyuan Medical Laboratory Co.,Ltd. Patentee before: Beijing Weiyuan Medical Laboratory Co.,Ltd. |