CN218491744U - Sample detection pretreatment equipment and reagent cartridges therein - Google Patents
Sample detection pretreatment equipment and reagent cartridges therein Download PDFInfo
- Publication number
- CN218491744U CN218491744U CN202221497556.2U CN202221497556U CN218491744U CN 218491744 U CN218491744 U CN 218491744U CN 202221497556 U CN202221497556 U CN 202221497556U CN 218491744 U CN218491744 U CN 218491744U
- Authority
- CN
- China
- Prior art keywords
- liquid storage
- liquid
- sample
- cartridge
- tube
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
本发明涉及一种检测试剂卡盒及设备,属于分子诊断设备技术领域。该检测试剂卡盒包括:储液转盘,设有若干个用于容纳液体的液体槽和用于容纳样本管的样本管孔;移液装置包括用于吸放液体的移液器;以及盒体,储液转盘安装于盒体内,并将盒体内部空间划分为储液转盘盘面以上的上腔体和储液转盘盘面以下的下腔体,所述储液转盘可沿所述盒体内壁做旋转运动及升降运动;移液装置固定于所述盒体上,所述储液转盘与所述盒体内壁使所述上腔体形成密闭空间;所述盒体上设有与所述上腔体连通的样品入口和匹配该样品入口的卡盒盖。该卡盒具备检测速度快密封性好的优点,不会产生交叉感染,解决了污染的问题。
The invention relates to a detection reagent cartridge and equipment, belonging to the technical field of molecular diagnostic equipment. The detection reagent cartridge includes: a liquid storage carousel, which is provided with several liquid tanks for containing liquids and sample tube holes for containing sample tubes; the pipetting device includes a pipette for sucking and discharging liquid; and a box body , the liquid storage turntable is installed in the box body, and the inner space of the box body is divided into an upper cavity above the surface of the liquid storage turntable and a lower cavity below the surface of the liquid storage turntable, and the liquid storage turntable can be made along the inner wall of the box Rotational movement and lifting movement; the liquid pipetting device is fixed on the box body, and the liquid storage turntable and the inner wall of the box form a closed space for the upper cavity; the box body is provided with the upper cavity body-connected sample inlet and a matching cartridge lid for the sample inlet. The cartridge has the advantages of fast detection speed and good sealing performance, and will not cause cross-infection, which solves the problem of contamination.
Description
技术领域technical field
本发明涉及分子诊断设备技术领域,特别是涉及一种样本检测前处理设备及其中的试剂卡盒。The invention relates to the technical field of molecular diagnostic equipment, in particular to a sample detection pretreatment equipment and a reagent cartridge therein.
背景技术Background technique
分子诊断病原体的检测过程,需要对病原体核酸进行裂解、洗涤、洗脱、分离纯化等过程的处理,且病原体的纯化的步骤繁杂、费时长、提取效率低,如果在一个开放式的环境下进行上述步骤,容易产生气溶胶、飞溅等样本间的污染,或者医护人员被病原体感染,并且还会影响提取物的纯度,进而影响实验结果的准确性。The detection process of pathogens in molecular diagnostics requires cleavage, washing, elution, separation and purification of pathogenic nucleic acids, and the purification steps of pathogens are complicated, time-consuming and low in extraction efficiency. If it is carried out in an open environment The above steps are prone to contamination between samples such as aerosols and splashes, or medical staff are infected by pathogens, and will also affect the purity of the extract, thereby affecting the accuracy of the experimental results.
基于上述原因,常规技术中,也有一些针对封闭式卡盒的研究,如分子诊断设备技术中,已实现了将多步骤的病原体检测方法整合到一个系统中,用户只需要将样本加入该系统,余下的步骤全部由系统自动完成。Based on the above reasons, in the conventional technology, there are also some researches on closed cartridges. For example, in the molecular diagnostic equipment technology, the multi-step pathogen detection method has been integrated into one system. The user only needs to add the sample to the system. The remaining steps are all automatically completed by the system.
因此,分子诊断的卡盒和微流控芯片采用密闭的、一次性使用的检测卡或检测管,较好地控制了交叉和携带污染。但这些检测卡或检测管往往一次只能检测一种病原体。Therefore, the cartridges and microfluidic chips for molecular diagnosis adopt airtight, single-use detection cards or detection tubes, which better control cross-over and carry-over contamination. But these test cards, or tubes, often only detect one pathogen at a time.
常规技术中,也有一些一体化病原体检测卡盒,能够在一个封闭的体系中实现对待测核酸的提取、扩增与检测,极大地避了样本在提取及扩增过程中的污染,有效的提高了核酸检测的准确性与可操作性。In the conventional technology, there are also some integrated pathogen detection cartridges, which can realize the extraction, amplification and detection of the nucleic acid to be tested in a closed system, which greatly avoids the contamination of the sample during the extraction and amplification process, and effectively improves The accuracy and operability of nucleic acid detection are improved.
发明内容Contents of the invention
基于此,有必要提供一种检测试剂卡盒,该卡盒具备密封性好的优点,可同时检测多种样品、同时兼容多种试剂、可对各种试剂开放兼容,无须将试剂封装在卡盒内,对不同试剂具有通用性,且不会产生交叉感染,解决了病原微生物的污染与操作员感染的问题。Based on this, it is necessary to provide a detection reagent cartridge, which has the advantages of good sealing, can detect multiple samples at the same time, is compatible with multiple reagents, can be open and compatible with various reagents, and does not need to pack the reagents in the card Inside the box, it is versatile for different reagents and will not cause cross-infection, which solves the problems of pathogenic microorganism contamination and operator infection.
一种检测试剂卡盒,包括:A detection reagent cartridge, comprising:
储液转盘,所述储液转盘上设有若干个用于容纳液体的液体槽和用于容纳样本管的样本管孔,所述样本管孔和若干个所述液体槽样品环绕所述储液转盘的旋转轴设置;A liquid storage turntable, the liquid storage turntable is provided with several liquid tanks for containing liquids and sample tube holes for containing sample tubes, the sample tube holes and several samples of the liquid tanks surround the liquid storage The rotation axis setting of the turntable;
移液装置,包括用于吸放液体的移液器,所述移液器的移液头位置与所述液体槽位置相对应;以及A liquid pipetting device, comprising a pipette for sucking and discharging liquid, the position of the pipetting head of the pipette corresponds to the position of the liquid tank; and
盒体,所述储液转盘安装于所述盒体内,并将所述盒体内部空间划分为储液转盘盘面以上的上腔体和储液转盘盘面以下的下腔体,所述储液转盘可沿所述盒体内壁做旋转运动及升降运动;所述移液装置固定于所述盒体上,且所述移液头位于所述上腔体内,所述储液转盘与所述盒体内壁使所述上腔体形成密闭空间;所述盒体上设有与所述上腔体连通的样品入口和匹配该样品入口的卡盒盖。A box body, the liquid storage turntable is installed in the box body, and the inner space of the box body is divided into an upper cavity above the surface of the liquid storage turntable and a lower cavity below the surface of the liquid storage turntable, the liquid storage turntable Rotational movement and lifting movement can be performed along the inner wall of the box; the pipetting device is fixed on the box body, and the pipetting head is located in the upper cavity, and the liquid storage turntable and the box body The wall makes the upper cavity form a closed space; the box body is provided with a sample inlet communicating with the upper cavity and a cartridge cover matching the sample inlet.
上述检测试剂卡盒,配备有卡盒盖,通过样品入口完成所有加样之后,即可将卡盒盖紧,之后的样品前处理过程均在卡盒内部完成,卡盒实现密闭,全程无污染。且上述检测试剂卡盒,可通过储液转盘的转动和样品入口的配合,无需预封装试剂在卡盒内部,便于该卡盒调整用于不同的检测项目中。The above detection reagent cartridge is equipped with a cartridge cover. After all the samples are added through the sample inlet, the cartridge can be closed tightly. The subsequent sample pretreatment process is completed inside the cartridge. The cartridge is airtight and pollution-free throughout the process. . Moreover, the detection reagent cartridge mentioned above can cooperate with the rotation of the liquid storage turntable and the sample inlet, without the need for pre-packaged reagents inside the cartridge, which facilitates the adjustment of the cartridge for different detection items.
在其中一个实施例中,所述液体槽包括试剂槽、反应槽、废液槽。可以理解的,对于具体液体槽中各类型槽位数量和作用等设置,可根据具体检测项目的要求设置,如产生废液较多的检测项目,可设计较大的废液槽等。In one of the embodiments, the liquid tank includes a reagent tank, a reaction tank, and a waste liquid tank. It can be understood that the setting of the number and function of various types of slots in a specific liquid tank can be set according to the requirements of specific testing items. For example, for testing items that generate more waste liquid, a larger waste liquid tank can be designed.
在其中一个实施例中,若干所述液体槽和所述样本管孔围绕所述储液转盘轴心构成圆环状布置,所述移液头和所述样品入口均与所述圆环位置相对应。按照上述设置,可将所述移液器及其移液头固定,通过储液转盘的旋转使所有液体槽位和样本管孔均能与固定的移液头对应,避免了需要移动移液头所造成的吸液误差或操作难度。而常规技术中的移液头通常与吸液设备分离并用软管连接,移液头移动过程中有折管的风险,导致移液精度不够,或者吸液不到的问题。In one of the embodiments, several of the liquid tanks and the sample tube holes form an annular arrangement around the axis of the liquid storage carousel, and the pipette head and the sample inlet are both in the same position as the annular ring. correspond. According to the above settings, the pipette and its pipetting head can be fixed, and all the liquid slots and sample tube holes can correspond to the fixed pipetting head through the rotation of the liquid storage turntable, avoiding the need to move the pipetting head The resulting suction error or operational difficulty. However, the pipetting head in the conventional technology is usually separated from the liquid suction device and connected with a hose. There is a risk of pipe breaking during the movement of the pipetting head, resulting in insufficient pipetting accuracy or insufficient suction.
在其中一个实施例中,所述移液器为活塞式移液器。通过移液头的固定不动,且移液器为移液活塞一体化式设计,使得外部驱动更加简单,仅需驱动活塞在单一方向上运动即可,具有操作可靠、稳定的优势。可以理解的,所述活塞式移液器可以选用如注射器等设备,而对于精度的要求,可通过控制管径大小,即活塞移动相同距离,所能移取液体量体积进行调整。In one of the embodiments, the pipette is a piston pipette. With the immobilization of the pipetting head and the integrated design of the pipetting piston, the external drive is simpler. It only needs to drive the piston to move in a single direction, which has the advantages of reliable and stable operation. It can be understood that the piston-type pipette can be equipped with equipment such as a syringe, and the accuracy requirement can be adjusted by controlling the diameter of the pipe, that is, the volume of the liquid that can be pipetted by the piston moving the same distance.
在其中一个实施例中,所述移液头为两个,所述储液转盘上还设有避位孔,当一个所述移液头与所述液体槽位置相对应时,另一个所述移液头与所述避位孔位置相对应。可以理解的,由于储液转盘上移时移液头位置不变,因此如有两个移液头,当使用一个移液头移液,另一个闲置时,如闲置移液头无对应孔位放置,则会与所述储液转盘的盘面相抵触,因而需考虑移液头的避位设置。而具体在什么位置设置避位孔,本领域技术人员可根据具体的检测项目流程需要,以及所适用的移液头数量和位置进行设置,该避位孔既可以为专门设计的孔位,也可以是不影响检测流程的其它试剂槽等。In one of the embodiments, there are two pipetting heads, and an escape hole is also provided on the liquid storage carousel. When one of the pipetting heads corresponds to the position of the liquid tank, the other of the pipetting heads The pipetting head corresponds to the position of the avoidance hole. It can be understood that the position of the pipette head remains unchanged when the storage carousel moves up, so if there are two pipette heads, when one pipette head is used to pipette and the other is idle, there will be no corresponding hole position for the idle pipette head If placed, it will conflict with the disk surface of the liquid storage carousel, so the avoidance setting of the pipetting head needs to be considered. As for where to set the avoidance hole, those skilled in the art can set it according to the needs of the specific detection project process, as well as the number and position of the applicable pipetting head. The avoidance hole can be a specially designed hole position, or It can be other reagent tanks, etc. that do not affect the detection process.
同时可以理解的,所述移液头的数量并不受限制,1个移液头也可以运行完成所有流程, 2个移液头能有效的避免样本的杂质带到纯净的样品内,或者更多的移液头也可以完成所有流程,但移液头的数量会影响储液转盘的大小,以及避位孔的数量,从而影响整个卡盒的大小,可根据具体检测项目要求而设定。At the same time, it can be understood that the number of pipette heads is not limited, and one pipette head can also run to complete all the processes, and two pipette heads can effectively prevent the impurities of the sample from being brought into the pure sample, or more Many pipetting heads can also complete all processes, but the number of pipetting heads will affect the size of the storage carousel and the number of avoidance holes, thus affecting the size of the entire cartridge, which can be set according to the requirements of specific testing items.
在其中一个实施例中,所述盒体内壁还设有用于限制所述储液转盘下移的限位件,所述限位件设于所述下腔体内。所述限位件用于避免所述储液转盘过度下移后脱出所述盒体,可以理解的,该限位件的具体形状和位置可根据本领域的常规技术设置即可。In one of the embodiments, the inner wall of the box is further provided with a limiting member for limiting the downward movement of the liquid storage turntable, and the limiting member is arranged in the lower cavity. The limiter is used to prevent the liquid storage carousel from falling out of the box after excessive downward movement. It can be understood that the specific shape and position of the limiter can be set according to conventional techniques in the art.
在其中一个实施例中,所述储液转盘与所述盒体内壁之间设有转盘密封圈,所述储液转盘与所述盒体形成动密封。可以理解的,也可通过本领域的其它手段实现上腔体的密封要求,但通过转盘密封圈实现动密封,具有方便可靠,且不影响储液转盘移动的优势。In one of the embodiments, a turntable sealing ring is provided between the liquid storage turntable and the inner wall of the box, and the liquid storage turntable forms a dynamic seal with the box body. It can be understood that the sealing requirements of the upper cavity can also be achieved by other means in the field, but the dynamic sealing is realized by the rotary disc sealing ring, which is convenient and reliable, and has the advantage of not affecting the movement of the liquid storage rotary disc.
在其中一个实施例中,该检测试剂卡盒还包括导向支架,所述储液转盘通过所述导向支架安装于所述盒体内,所述转盘密封圈套设于所述导向支架外周边缘。可以理解的,通过导向支架实现储液转盘的导向并带动所述储液转盘的运动,具有运动稳定,安装便利的优势。In one embodiment, the detection reagent cartridge further includes a guide bracket, the liquid storage turntable is installed in the box through the guide bracket, and the turntable sealing ring is sleeved on the outer peripheral edge of the guide bracket. It can be understood that the guidance of the liquid storage turntable and the movement of the liquid storage turntable are realized by the guide bracket, which has the advantages of stable movement and convenient installation.
在其中一个实施例中,该检测试剂卡盒还包括用于密封所述储液转盘的密封盖,所述密封盖安装于所述导向支架上,所述储液转盘可与所述密封盖相对转动。通过密封盖的运用,可在完成样本的处理后将所述储液转盘密封,实现整体移除,进一步降低了产生污染的风险。且所述储液转盘和所述密封盖均安装于导向支架上,二者之间可相对转动,不影响储液转盘的旋转。In one of the embodiments, the detection reagent cartridge further includes a sealing cover for sealing the liquid storage carousel, the sealing cover is installed on the guide bracket, and the liquid storage carousel can be opposite to the sealing cover turn. Through the use of the sealing cover, the liquid storage carousel can be sealed after the processing of the sample is completed, so as to realize the overall removal and further reduce the risk of contamination. Moreover, both the liquid storage turntable and the sealing cover are installed on the guide bracket, and they can rotate relative to each other without affecting the rotation of the liquid storage turntable.
在其中一个实施例中,该检测试剂卡盒还包括弹性复位件,所述弹性复位件一端抵靠于所述密封盖上,另一端抵靠于所述盒体内壁,且所述弹性复位件位于所述上腔体内。可通过如弹簧等弹性复位件间接对所述储液转盘施加向下的力,实现移液装置取液后使储液转盘下移复位的目的。In one of the embodiments, the detection reagent cartridge further includes an elastic reset member, one end of the elastic reset member leans against the sealing cover, and the other end leans against the inner wall of the box, and the elastic reset member located in the upper cavity. A downward force can be indirectly exerted on the liquid storage turntable through an elastic return member such as a spring, so that the liquid storage turntable moves down and resets after the pipetting device takes liquid.
在其中一个实施例中,所述盒体内壁还设有用于为导向支架导向的导向杆,所述密封盖上设有与所述导向杆配合的导向套,所述导向杆和导向套均设于所述上腔体内。可通过将导向杆套设在导向杆外实现导向作用。In one of the embodiments, the inner wall of the box is also provided with a guide rod for guiding the guide bracket, and the sealing cover is provided with a guide sleeve that cooperates with the guide rod, and the guide rod and the guide sleeve are both provided with in the upper cavity. The guiding effect can be realized by sheathing the guide rod outside the guide rod.
在其中一个实施例中,所述密封盖安装于所述导向支架上并具有工作状态位和密封状态位,当所述密封盖处于所述工作状态位,所述密封盖与所述储液转盘之间可相对转动,当所述密封盖处于所述密封状态位,所述密封盖与所述储液转盘卡扣配合并密封所述液体槽,所述密封盖上设有至少两个取液孔,所述取液孔位置分别与所述移液头和所述样品入口位置相对应。可以理解的,当样本在所述卡盒内进行前处理移液等步骤时,所述移液头或外部机构可通过所述取液孔取样及加样;而当样本完成前处理步骤后,可通过旋转所述储液转盘使液体槽和取液孔等错位并卡扣配合固定,而实现密封所述液体槽的目的。In one of the embodiments, the sealing cover is installed on the guide bracket and has a working state position and a sealing state position. When the sealing cover is in the working state position, the sealing cover and the liquid storage turntable They can be rotated relative to each other. When the sealing cover is in the sealing position, the sealing cover is snap-fitted with the liquid storage turntable and seals the liquid tank. At least two liquid-taking The position of the liquid-taking hole corresponds to the position of the pipetting head and the position of the sample inlet respectively. It can be understood that when the sample is subjected to pre-processing steps such as pipetting in the cartridge, the pipetting head or external mechanism can take samples and add samples through the liquid-taking hole; and when the sample completes the pre-processing steps, The purpose of sealing the liquid tank can be realized by rotating the liquid storage turntable to displace the liquid tank and the liquid taking hole and fix them with snap fit.
在其中一个实施例中,该检测试剂卡盒还包括样本管,所述样本管包括卡接配合的管盖和管体,所述管体通过卡扣连接固定于所述样本管孔内,所述密封盖上设有密封顶盖,所述管盖通过紧配合可拆卸式连接所述密封顶盖。所述样本管用于盛装经前处理完成的样品,通过卡接配合的管盖和管体,可在前处理完成后,将样品转移至管体内,并利用密封顶盖将管盖扣合于管体上,再将密闭扣合的样本管转移至其它设备完成检测。此时样本管与卡盒进行了分离,且分离后两者都实现了密封。In one of the embodiments, the detection reagent cartridge further includes a sample tube, and the sample tube includes a snap-fit cap and a tube body, and the tube body is fixed in the hole of the sample tube through a snap connection, so that A sealing top cover is provided on the sealing cover, and the tube cover is detachably connected to the sealing top cover through tight fit. The sample tube is used to contain the pre-treated sample, and the tube cover and tube body can be snapped together to transfer the sample to the tube body after the pre-treatment is completed, and the tube cover is fastened to the tube by the sealing top cover. body, and then transfer the sealed sample tube to other equipment to complete the test. At this point, the sample tube is separated from the cartridge, and both are sealed after separation.
如此样本管为PCR反应管,后续需进行扩增和检测项目,则PCR反应管分离后,即可以在qPCR仪器上实现扩增和荧光检测,实时荧光定量PCR仪(qPCR)同时检测多个样本时(1个样本1个项目对应1个PCR反应管),体积较小。避免了PCR反应管与卡盒不分离,直接上qPCR仪,存在和仪器不匹配,检测结果不准确及占用空间位置大,不利于多样本检测的缺陷。In this case, the sample tube is a PCR reaction tube, and subsequent amplification and detection items are required. After the PCR reaction tube is separated, the amplification and fluorescence detection can be realized on the qPCR instrument, and the real-time fluorescent quantitative PCR instrument (qPCR) can detect multiple samples at the same time. Time (1 sample and 1 item corresponds to 1 PCR reaction tube), and the volume is small. It avoids the defects that the PCR reaction tubes are not separated from the cartridges, and are directly loaded into the qPCR instrument. There is a mismatch with the instrument, inaccurate test results, and a large space occupation, which is not conducive to multi-sample detection.
同时,对于样本管的数量也不受限定,其数量可以为n,即实现1个样本对应n个检测项目的联检。At the same time, the number of sample tubes is not limited, and the number can be n, that is, the joint inspection of one sample corresponding to n detection items is realized.
在其中一个实施例中,所述管体外壁设有沟槽,所述沟槽内设有管体密封圈,所述管体与所述储液转盘动密封。In one of the embodiments, a groove is provided on the outer wall of the tube, and a tube body sealing ring is provided in the groove, and the tube body is dynamically sealed with the liquid storage turntable.
在其中一个实施例中,该检测试剂卡盒还包括管盖顶入机构,所述管盖顶入机构固定于所述盒体内壁且朝所述储液转盘方向凸起。可通过所述顶入机构顶推设于所述密封盖上的密封顶盖再使所述管盖与所述管体卡扣固定,实现管盖和管体的密封。In one embodiment, the detection reagent cartridge further includes a tube cover pushing mechanism, and the tube cover pushing mechanism is fixed on the inner wall of the box and protrudes toward the liquid storage turntable. The sealing top cover provided on the sealing cover can be pushed by the pushing mechanism, and then the tube cover and the tube body can be buckled and fixed, so as to realize the sealing of the tube cover and the tube body.
本发明还公开了一种样本检测前处理设备,包括上述的检测试剂卡盒,以及驱动装置,所述驱动装置包括升降装置和旋转装置,所述旋转装置驱动所述储液转盘沿其旋转轴旋转,所述升降装置驱动所述储液转盘升降。The present invention also discloses a pre-processing device for sample detection, including the above-mentioned detection reagent cartridge, and a driving device, the driving device includes a lifting device and a rotating device, and the rotating device drives the liquid storage turntable along its rotation axis Rotate, the lifting device drives the liquid storage turntable to lift.
上述样本检测前处理设备,以升降装置和旋转装置驱动储液转盘实现升降和旋转,以完成移液促使样本进行前处理反应。The above-mentioned pre-processing equipment for sample detection uses a lifting device and a rotating device to drive the liquid storage carousel to realize lifting and rotating, so as to complete the liquid transfer and promote the sample to undergo a pre-treatment reaction.
在其中一个实施例中,所述升降装置包括推杆和推杆驱动器,所述推杆一端安装于所述推杆驱动器上,另一端顶靠所述储液转盘,通过所述推杆驱动器驱动所述推杆收缩或伸长驱动所述储液转盘上下移动;所述旋转装置包括储液转盘中心轴和驱动该中心轴旋转的驱动装置。例如,可以外部装置夹取转所述中心轴,带动储液转盘做圆周运动。In one of the embodiments, the lifting device includes a push rod and a push rod driver, one end of the push rod is installed on the push rod driver, and the other end is against the liquid storage turntable, driven by the push rod driver The push rod shrinks or extends to drive the liquid storage turntable to move up and down; the rotation device includes a central axis of the liquid storage turntable and a driving device for driving the central axis to rotate. For example, the central shaft can be clamped and rotated by an external device to drive the liquid storage turntable to perform circular motion.
在其中一个实施例中,该样本检测前处理设备还包括移液驱动装置,所述移液器内设有活塞杆和弹性活塞,所述弹性活塞上设有与所述活塞杆紧配合的杆孔,所述移液驱动装置为用于驱动所述活塞杆运动的抓取装置。In one of the embodiments, the pre-processing equipment for sample detection also includes a pipetting drive device, the pipette is provided with a piston rod and an elastic piston, and the elastic piston is provided with a rod tightly fitted with the piston rod hole, and the pipetting drive device is a grasping device for driving the movement of the piston rod.
在其中一个实施例中,该样本检测前处理设备还包括孵育震荡装置、磁吸装置和移动机构,所述液体槽包括试剂槽、反应槽和废液槽,所述孵育震荡装置和磁吸装置均可在所述移动机构的带动下靠近或远离所述反应槽。可以理解的,如所述样本检测为分子诊断检测,该前处理设备包括用于核酸提取、纯化的孵育震荡装置和磁吸装置等,并利用常规的移动机构实现上述装置的移动,在需要进行孵育震荡或磁吸时,使相应装置靠近或包覆相应的液体槽 (如反应槽),以完成前处理工作。In one of the embodiments, the pre-processing equipment for sample detection also includes an incubation vibration device, a magnetic suction device and a moving mechanism, the liquid tank includes a reagent tank, a reaction tank and a waste liquid tank, and the incubation vibration device and the magnetic suction device All can approach or move away from the reaction tank driven by the moving mechanism. It can be understood that if the sample detection is a molecular diagnostic test, the pre-processing equipment includes an incubation and vibration device for nucleic acid extraction and purification, a magnetic suction device, etc., and the movement of the above-mentioned devices is realized by using a conventional moving mechanism. When incubating with shaking or magnetic suction, make the corresponding device close to or cover the corresponding liquid tank (such as the reaction tank) to complete the pretreatment work.
在其中一个实施例中,该样本检测前处理设备还包括机械抓手,所述机械抓手用于抓取所述样本管。可以理解的,所述机械抓手采用常规机械元件,按照本领域通常设置即可,但通过机械抓手配合样本管的设计,使该前处理设备既实现了全自动化操作,又具有占用空间小,设备灵活性高的优势。In one of the embodiments, the pre-processing device for sample detection further includes a mechanical gripper for grabbing the sample tube. It can be understood that the mechanical gripper adopts conventional mechanical elements, which can be set according to the usual settings in this field. However, through the design of the mechanical gripper and the sample tube, the pre-processing equipment can realize fully automatic operation and occupy a small space. , The advantages of high equipment flexibility.
本发明还公开了一种样本检测前处理方法,采用上述的前处理设备,包括以下步骤:The present invention also discloses a pre-processing method for sample detection, using the above-mentioned pre-processing equipment, including the following steps:
加样:将所述卡盒盖开启,控制所述旋转装置驱动所述储液转盘旋转至所述液体槽与所述样品入口对齐,将待处理样品及试剂加入,并将所述卡盒盖盖紧;Adding samples: Open the cartridge cover, control the rotating device to drive the liquid storage turntable to rotate until the liquid tank is aligned with the sample inlet, add the sample to be processed and the reagent, and put the cartridge cover cover tightly;
反应:控制所述旋转装置驱动所述储液转盘旋转,使所述移液头位置对应于所需液体槽位置,控制所述升降装置驱动所述储液转盘上升,使所述移液头位于对应液体槽内,控制所述移液装置吸液,控制所述升降装置使所述储液转盘下降,控制所述储液转盘旋转至预定位置,完成移液,并使样本在所述液体槽内完成前处理反应;Response: Control the rotating device to drive the liquid storage turntable to rotate, so that the position of the pipetting head corresponds to the position of the required liquid tank, and control the lifting device to drive the liquid storage turntable to rise, so that the pipetting head is at Corresponding to the liquid tank, control the pipetting device to absorb liquid, control the lifting device to lower the liquid storage turntable, control the liquid storage turntable to rotate to a predetermined position, complete the pipetting, and make the sample in the liquid tank Complete the pretreatment reaction within;
取样:控制所述移液装置将经前处理的样品溶液吸出,转移至样本管内,将该样本管取出。Sampling: control the pipetting device to suck out the pretreated sample solution, transfer it into a sample tube, and take out the sample tube.
与现有技术相比,本发明具有以下有益效果:Compared with the prior art, the present invention has the following beneficial effects:
本发明的一种检测试剂卡盒,配备有卡盒盖,通过样品入口完成所有加样之后,即可将卡盒盖紧,之后的样品前处理过程均在卡盒内部完成,卡盒实现密闭,全程无污染。且上述检测试剂卡盒,可通过储液转盘的转动和样品入口的配合,无需预封装试剂在卡盒内部,便于该卡盒调整用于不同的检测项目中。A detection reagent cartridge of the present invention is equipped with a cartridge cover. After all the samples are added through the sample inlet, the cartridge can be tightly covered, and the subsequent sample pretreatment process is completed inside the cartridge, and the cartridge is sealed. , No pollution in the whole process. Moreover, the detection reagent cartridge mentioned above can cooperate with the rotation of the liquid storage turntable and the sample inlet, without the need for pre-packaged reagents inside the cartridge, which facilitates the adjustment of the cartridge for different detection items.
通过采用储液转盘旋转的方式,使液体槽的轴线与移液头的轴线对齐,液体槽等与移液头都分布于该卡盒的同一个旋转中心,相对于平移移液头的方式,或者平移储液盘的方式,旋转的方式更省空间,平移的有移动后产生的存放空间,而旋转无需多余的存放空间,可将卡盒体积设计得更小,更节约空间。By adopting the method of rotating the liquid storage turntable, the axis of the liquid tank is aligned with the axis of the pipetting head, and the liquid tank and the pipetting head are distributed on the same rotation center of the cartridge. Compared with the way of translating the liquid pipetting head, Or the way of translating the liquid storage tray, the way of rotation is more space-saving, the storage space generated after the translation is moved, and the rotation does not require extra storage space, and the volume of the cartridge can be designed to be smaller and more space-saving.
且该卡盒加工制造、组装,均不涉及高难度元件设备,具有制造和装配成本便宜,良品率自主可控的优势。Moreover, the manufacturing and assembly of the cartridge do not involve high-difficulty component equipment, which has the advantages of low manufacturing and assembly costs and independent controllable yield rate.
附图说明Description of drawings
图1为检测试剂卡盒结构示意图一;Fig. 1 is a schematic diagram of the structure of the detection reagent cartridge;
图2为检测试剂卡盒结构示意图二;Fig. 2 is the second schematic diagram of the structure of the detection reagent cartridge;
图3为检测试剂卡盒部分结构爆炸示意图;Figure 3 is a schematic exploded view of a partial structure of the detection reagent cartridge;
图4为检测试剂卡盒外部结构示意图;Figure 4 is a schematic diagram of the external structure of the detection reagent cartridge;
图5为储液转盘结构示意图;Fig. 5 is a structural schematic diagram of a liquid storage turntable;
图6为移液装置和导向杆结构示意图;Fig. 6 is a structural schematic diagram of a pipetting device and a guide rod;
图7为样本管结构示意图;Fig. 7 is a schematic diagram of the sample tube structure;
图8为储液转盘和样本管俯视图;Figure 8 is a top view of the liquid storage carousel and the sample tube;
图9为图8的EE剖面图;Fig. 9 is the EE sectional view of Fig. 8;
图10为密封盖俯视图;Figure 10 is a top view of the sealing cover;
图11为密封盖DD剖面图;Figure 11 is a sectional view of the sealing cover DD;
图12为检测试剂卡盒内储液转盘运动方向示意图。Fig. 12 is a schematic diagram of the movement direction of the liquid storage turntable in the detection reagent cartridge.
其中:100、储液转盘;110、反应槽;120、废液槽;131、第一试剂槽;132、第二试剂槽;133、第三试剂槽;134、第四试剂槽;141、第一避位孔;142、第二避位孔;143、第三避位孔;144、第四避位孔;145、第五避位孔;150、样本管孔;200、移液装置;211、1号移液头;212、2号移液头;300、盒体;310、样品入口;320、卡盒盖;330、顶入机构;400、样本管;410、管盖;420、管体;421、管体密封圈;500、导向支架;510、转盘密封圈;520、弹性复位件;530、限位件;540、导向杆;600、密封盖;610、取液孔;620、导向套;630、密封顶盖。Among them: 100, liquid storage turntable; 110, reaction tank; 120, waste liquid tank; 131, first reagent tank; 132, second reagent tank; 133, third reagent tank; 134, fourth reagent tank; 141, first reagent tank One escape hole; 142, the second escape hole; 143, the third escape hole; 144, the fourth escape hole; 145, the fifth escape hole; 150, the sample tube hole; 200, the pipetting device; 211 , No. 1 pipetting head; 212, No. 2 pipetting head; 300, box body; 310, sample inlet; 320, cartridge cover; 330, jacking mechanism; 400, sample tube; 410, tube cover; 420, tube body; 421, pipe body sealing ring; 500, guide bracket; 510, turntable sealing ring; 520, elastic reset piece; 530, limit piece; 540, guide rod; 600, sealing cover; Guide sleeve; 630, sealing top cover.
具体实施方式Detailed ways
为了便于理解本发明,下面将参照相关附图对本发明进行更全面的描述。附图中给出了本发明的较佳实施例。但是,本发明可以以许多不同的形式来实现,并不限于本文所描述的实施例。相反地,提供这些实施例的目的是使对本发明的公开内容的理解更加透彻全面。In order to facilitate the understanding of the present invention, the present invention will be described more fully below with reference to the associated drawings. Preferred embodiments of the invention are shown in the accompanying drawings. However, the present invention can be embodied in many different forms and is not limited to the embodiments described herein. On the contrary, these embodiments are provided to make the understanding of the disclosure of the present invention more thorough and comprehensive.
需要说明的是,当元件被称为“固定于”另一个元件,它可以直接在另一个元件上或者也可以存在居中的元件。当一个元件被认为是“安装”另一个元件,它可以是直接安装到另一个元件或者可能同时存在居中元件。It should be noted that when an element is referred to as being “fixed” to another element, it can be directly on the other element or there can also be an intervening element. When an element is said to be "mounted" to another element, it can be directly mounted to the other element or intervening elements may also be present.
除非另有定义,本文所使用的所有的技术和科学术语与属于本发明的技术领域的技术人员通常理解的含义相同。本文中在本发明的说明书中所使用的术语只是为了描述具体的实施例的目的,不是旨在于限制本发明。本文所使用的术语“和/或”包括一个或多个相关的所列项目的任意的和所有的组合。Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field of the invention. The terms used herein in the description of the present invention are for the purpose of describing specific embodiments only, and are not intended to limit the present invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
实施例1Example 1
一种检测试剂卡盒,如图1-4包括:储液转盘100,移液装置200和盒体300。且根据需要,可以配备或不配备专门的样本管400,在本实施例中,以配备样本管400为例进行说明。A detection reagent cartridge, as shown in FIGS. 1-4 , includes: a
所述储液转盘100上设有若干个用于容纳液体的液体槽和用于容纳样本管400的样本管孔150,所述样本管孔150和若干个所述液体槽样品环绕所述储液转盘100的旋转轴设置。具体的,若干所述液体槽和所述样本管孔150围绕所述储液转盘100轴心构成圆环状布置,所述移液头和所述样品入口310分别于所述圆环位置相对应。The
在本实施例中,所述液体槽包括试剂槽(第一试剂槽131、第二试剂槽132、第三试剂槽 133、第四试剂槽134、)、反应槽110、废液槽120。可以理解的,对于具体液体槽中各类型槽位数量和作用等设置,可根据具体检测项目的要求设置,如产生废液较多的检测项目,可设计较大的废液槽等,以避免后续处理过程中,如需要对样品进行震荡等操作而导致的漏液或污染。在本实施例中,该检测试剂卡盒还包括导向支架500,所述储液转盘100通过所述导向支架500安装于所述盒体300内,所述转盘密封圈510套设于所述导向支架500外周边缘,使所述导向支架500带动储液转盘100与所述盒体300形成动密封。In this embodiment, the liquid tanks include reagent tanks (the first reagent tank 131, the
对于储液转盘100的下移限位,盒体300内壁还设有用于限制所述储液转盘100下移的限位件530,所述限位件530设于所述下腔体内。所述限位件530用于避免所述储液转盘100 过度下移后脱出所述盒体300,可以理解的,该限位件530的具体形状和位置可根据本领域的常规技术设置即可,在本实施例中,该限位件530为环绕安装在卡盒内壁的限位板。For limiting the downward movement of the
所述移液装置200包括用于吸放液体的活塞式移液器(如与注射器结构类似的活塞式吸液件),所述移液器的移液头位置与所述液体槽位置相对应。可以理解的,所述活塞式移液器可以选用如注射器等设备,而对于精度的要求,可通过控制管径大小,即活塞移动相同距离,所能移取液体量体积进行调整。The
在本实施例中,所述移液头为两个,所述储液转盘100上还设有避位孔,当一个所述移液头与所述液体槽位置相对应时,另一个所述移液头与所述避位孔位置相对应。可以理解的,由于储液转盘100上移时移液头位置不变,因此如有两个移液头,当使用一个移液头移液,另一个闲置时,如闲置移液头无对应孔位放置,则会与所述储液转盘100的盘面相抵触,因而需考虑移液头的避位设置。而具体在什么位置设置避位孔,本领域技术人员可根据具体的检测项目流程需要,以及所适用的移液头数量和位置进行设置,该避位孔既可以为专门设计的孔位,也可以是不影响检测流程的其它试剂槽等。In this embodiment, there are two pipetting heads, and an escape hole is also provided on the
根据本实施例中检测项目为分子诊断的要求,以及两个移液头的设计,如图5-6所示,本实施例中储液转盘100上孔位的设置以容纳待处理样品溶液及进行反应的反应槽110开始,沿储液转盘100圆周方向依次设有反应槽110、第四避位孔144、废液槽120、第三避位孔143、第四试剂槽134、第二试剂槽132、第三试剂槽133、第一避位孔141、第二避位孔142、样本管孔150以及第五避位孔145。According to the requirement that the detection item in this embodiment is molecular diagnosis, and the design of the two pipetting heads, as shown in Figure 5-6, the hole position on the
上述储液转盘100通过导向支架500安装于所述盒体300内,并将所述盒体300内部空间划分为储液转盘100盘面以上的上腔体和储液转盘100盘面以下的下腔体,所述储液转盘 100可沿所述盒体300内壁做旋转运动及升降运动;所述移液装置200固定于所述盒体300 上,且所述移液头位于所述上腔体内,所述储液转盘100与所述盒体300内壁使所述上腔体形成密闭空间;所述盒体300上设有与所述上腔体连通的样品入口310和匹配该样品入口310 的卡盒盖320。The
为进一步降低污染的风险,本实施例的卡盒还包括用于密封所述储液转盘100的密封盖 600,所述密封盖600安装于所述导向支架500上,所述储液转盘100可与所述密封盖600相对转动。通过密封盖的运用,可在完成样本的处理后将所述储液转盘密封,实现整体移除,进一步降低了产生污染的风险。且所述储液转盘和所述密封盖均安装于导向支架上,二者之间可相对转动,不影响储液转盘的旋转。In order to further reduce the risk of contamination, the cartridge of this embodiment further includes a sealing
本实施例中,该检测试剂卡盒还包括弹性复位件520,所述弹性复位件520一端抵靠于所述密封盖600上,另一端抵靠于所述盒体上内壁。可通过如弹簧等弹性复位件520间接对所述储液转盘100施加向下的力,实现移液装置取液后使储液转盘下移复位的目的。In this embodiment, the detection reagent cartridge further includes an
所述盒体上内壁还设有向下延伸的用于为导向支架500导向的导向杆540,所述密封盖 600上设有与所述导向杆540配合的导向套620,所述导向杆540和导向套620均设于所述上腔体内。可通过将导向杆套设在导向杆外实现导向作用。且所述弹性复位件520(弹簧)也套装于所述导向套620外。The upper inner wall of the box body is also provided with a
具体的,所述密封盖600安装于所述导向支架500上并具有工作状态位和密封状态位,当所述密封盖600处于所述工作状态位,所述密封盖600与所述储液转盘100之间可相对转动,当所述密封盖600处于所述密封状态位,所述密封盖600与所述储液转盘100卡扣配合并密封所述液体槽,所述密封盖600上设有至少两个取液孔610,所述取液孔610位置分别与所述移液头和所述样品入口310位置相对应。可以理解的,当样本在所述卡盒内进行前处理移液等步骤时,所述移液头或外部机构可通过所述取液孔610取样及加样;而当样本完成前处理步骤后,可通过旋转所述储液转盘100使液体槽和取液孔610等错位并卡扣配合固定,而实现密封所述液体槽的目的。Specifically, the sealing
本实施例中的样本管400包括卡接配合的管盖410和管体420,如图7-11所示,所述管体420通过卡扣连接固定于所述样本管孔150内,所述密封盖600上设有密封顶盖630,所述管盖410通过紧配合可拆卸式连接所述密封顶盖630。The
所述样本管400用于盛装经前处理完成的样品,通过卡接配合的管盖410和管体420,可在前处理完成后,将样品转移至管体420内,并利用密封盖600上的密封顶盖630将管盖410 扣合于管体420上,再将密闭扣合的样本管400转移至其它设备完成检测。此时样本管400 与卡盒进行了分离,且分离后两者都实现了密封。The
具体的,该管体420外壁设有沟槽,所述沟槽内设有管体420密封圈,所述管体420与所述储液转盘100动密封。Specifically, a groove is provided on the outer wall of the
为了实现将管盖410扣合于管体420上,该检测试剂卡盒还包括管盖410顶入机构330,所述管盖410顶入机构330固定于所述盒体300内壁且朝所述储液转盘100方向凸起。可通过所述顶入机构330顶推设于所述密封盖600上的密封顶盖630,挤压管盖410与所述管体420卡扣固定,实现管盖410和管体420的密封,进而实现了全自动化操作。In order to fasten the
实施例2Example 2
一种用于分子诊断的样本检测前处理设备,采用上述实施例1的检测试剂卡盒和驱动装置,所述驱动装置包括升降装置和旋转装置,所述旋转装置驱动所述储液转盘100沿其旋转轴旋转,所述升降装置驱动所述储液转盘100升降,如图12所示。A sample detection pretreatment device for molecular diagnosis, using the detection reagent cartridge and the driving device of the above-mentioned embodiment 1, the driving device includes a lifting device and a rotating device, and the rotating device drives the
上述样本检测前处理设备,以升降装置和旋转装置驱动储液转盘100实现升降和旋转,以完成移液促使样本进行前处理反应。The above-mentioned sample detection pre-processing equipment uses a lifting device and a rotating device to drive the
具体的,所述升降装置包括推杆和推杆驱动器,所述推杆一端安装于所述推杆驱动器上,另一端顶靠所述储液转盘100,通过所述推杆驱动器驱动所述推杆收缩或伸长驱动所述储液转盘100上下移动;所述旋转装置包括储液转盘100中心轴和驱动该中心轴旋转的驱动装置。例如,可以外部装置夹取转所述中心轴,带动储液转盘100做圆周运动。Specifically, the lifting device includes a push rod and a push rod driver, one end of the push rod is installed on the push rod driver, and the other end is against the
为实现自动化操作流程,该样本检测前处理设备还包括移液驱动装置,所述移液器内设有活塞杆和弹性活塞,所述弹性活塞上设有与所述活塞杆紧配合的杆孔,所述移液驱动装置为用于驱动所述活塞杆运动的抓取装置。In order to realize the automatic operation process, the pre-processing equipment for sample detection also includes a pipetting drive device, the pipette is provided with a piston rod and an elastic piston, and the elastic piston is provided with a rod hole tightly fitted with the piston rod , the pipetting driving device is a grasping device for driving the movement of the piston rod.
例如,活塞杆与卡盒内1号移液器中心轴线对齐,沿着轴线向下,直到顶至活塞到最底部,此时活塞杆的顶部会张紧至活塞(活塞为弹性材料)表面的孔内,实现活塞杆与活塞一体化,驱动带动活塞杆上下运动,实现1号移液器的移液。抓取装置同样抓取2号移液器的活塞杆,使活塞杆与活塞一体化,驱动抓取装置上下运动,实现2号移液头212的移液。For example, the piston rod is aligned with the center axis of pipette No. 1 in the cartridge, and goes down the axis until it reaches the bottom of the piston, at which point the top of the piston rod is tensioned to the surface of the piston (the piston is an elastic material) In the hole, the piston rod and the piston are integrated, and the piston rod is driven to move up and down to realize the pipetting of No. 1 pipette. The grasping device also grasps the piston rod of the No. 2 pipette so that the piston rod and the piston are integrated, and drives the grasping device to move up and down to realize the pipetting of the No. 2
由于本实施例的前处理设备用于分子诊断程序,该样本检测前处理设备还需要对样本进行核酸提取和纯化,如采用磁珠提取纯化,因此,该设备还包括孵育震荡装置、磁吸装置和移动机构,所述孵育震荡装置和磁吸装置均可在所述移动机构的带动下靠近或远离所述反应槽110。可以理解的,如所述样本检测为分子诊断检测,该前处理设备包括用于核酸提取、纯化的孵育震荡装置和磁吸装置等,并利用常规的移动机构实现上述装置的移动,在需要进行孵育震荡或磁吸时,使相应装置靠近或包覆相应的液体槽(如反应槽110),以完成前处理工作。Since the pretreatment equipment of this embodiment is used for molecular diagnostic procedures, the sample detection pretreatment equipment also needs to extract and purify the nucleic acid of the sample, such as using magnetic beads for extraction and purification. Therefore, the equipment also includes an incubation and shaking device, a magnetic suction device And the moving mechanism, the incubation and vibration device and the magnetic suction device can be driven by the moving mechanism to approach or move away from the
进一步的,该样本检测前处理设备还包括机械抓手,所述机械抓手用于抓取所述样本管 400。可以理解的,所述机械抓手采用常规机械元件,按照本领域通常设置即可,但通过机械抓手配合样本管400的设计,使该前处理设备既实现了全自动化操作,又具有占用空间小,设备灵活性高的优势。Further, the pre-processing equipment for sample detection also includes a mechanical gripper for grabbing the
实施例3Example 3
一种分子诊断检测样本前处理方法,采用上述实施例2的前处理设备,包括以下步骤:A method for pretreatment of molecular diagnostic test samples, using the pretreatment equipment of the above-mentioned embodiment 2, comprising the following steps:
一、加样。1. Adding samples.
将所述卡盒盖320开启,控制所述旋转装置驱动所述储液转盘100旋转至所述液体槽与所述样品入口310对齐,将待处理样品及试剂加入,并将所述卡盒盖320盖紧。在本实施例中具体如下:Open the
1、外部机构驱动旋开卡盒盖320;1. The external mechanism drives to unscrew the
2、此时储液转盘100上反应槽110与样品入口310的轴线对齐,卡盒外部自动化仪器移液枪依次加入300uL的RNA捕获液、20uL蛋白酶K、15uL磁珠悬液;2. At this time, the
3、转动储液转盘100,此时第二试剂槽132与样品入口310轴线对齐,卡盒外部自动化仪器移液枪一次性加入500uL的漂洗液PWC;3. Rotate the
4、转动储液转盘100,此时第三试剂槽133与样品入口310轴线对齐,卡盒外部自动化仪器移液枪一次性加入1000uL的漂洗液PWE;4. Rotate the
5、转动储液转盘100,此时第四试剂槽134与样品入口310轴线对齐,卡盒外部自动化仪器移液枪一次性加入200uL的无RNA酶双蒸水。5. Rotate the
6、转动储液转盘100,此时反应槽110与样品入口310轴线对齐,外部机构加入样本200uL (此加样本的过程需要在仪器一个装有高效过滤的封闭环境内进行);6. Rotate the
7、外部机构旋上卡盒盖320,整个卡盒完全密封。7. The external mechanism screws on the
二、前处理反应。Second, the pretreatment reaction.
控制所述旋转装置驱动所述储液转盘100旋转,使所述移液头位置对应于所需液体槽位置,控制所述升降装置驱动所述储液转盘100上升,使所述移液头位于对应液体槽内,控制所述移液装置200吸液,控制所述升降装置使所述储液转盘100下降,控制所述储液转盘100 旋转至预定位置,如图11所示,完成移液,并使样本在所述液体槽内完成前处理反应。在本实施例中具体如下:Control the rotating device to drive the
1、反应槽110加入样本后,反应槽110内有:样本+300uL的RNA捕获液+20uL蛋白酶K+15uL磁珠悬液;1. After the sample is added to the
2、在反应槽110位置加入一个孵育震荡装置,对反应槽110内液体进行孵育以及震荡,完成样本的裂解,释放出核酸,并震荡起来,让核酸吸附到磁珠之上;2. Add an incubation and shaking device at the position of the
3、反应槽110位置加入一个磁环,让吸附有核酸的磁珠吸附到反应槽110的侧壁之上; 4、转动储液转盘100,使1号移液头211轴线与反应槽110对齐,再将储液转盘100整体往上推动,推动1号移液头211的针尖与反应槽110的槽位底部只留微小间隙位置,驱动1号移液头211的活塞往上,吸取反应槽110除吸附于侧壁的核酸外的废液;(此时2号移液头 212在第四避位孔144位置);3. Add a magnetic ring to the position of the
5、驱动推杆收缩,抵靠导向支架500的弹簧推动储液转盘100整体往下走,直到储液转盘100到达储液转盘100限位件530,完成储液转盘100的向下复位;5. The push rod is driven to shrink, and the spring against the
6、转动储液转盘100,使废液槽120与1号移液头211轴线对齐,再将储液转盘100整体往上部推动,推动1号移液头211的针尖与废液槽120的孔位底部留有间隙的位置,驱动1号移液头211的活塞向下,排出1号移液头211内的废液至废液槽120;驱动推杆收缩,抵靠导向支架500的弹簧推动储液转盘100整体往下走,直到储液转盘100到达储液转盘100 限位件530,完成储液转盘100的向下复位;(此时2号移液头212在4#孔位置,4#孔装的是双蒸水,不会影响2号移液头212的洁净度)6. Rotate the
7、类似步骤4、步骤5,转动储液转盘100,使第二试剂槽132与1号移液头211轴线对齐,吸取500uL的漂洗液PWC;(此时2号移液头212在第一避位孔141)7. Similar to steps 4 and 5, rotate the
8、类似步骤6,转动储液转盘100,使反应槽110与1号移液头211轴线对齐,排出1号移液头211内的PWC至反应槽110;(此时2号移液头212在第四避位孔144);驱动震荡孵育、磁吸后,吸取废液,使废液槽120与1号移液头211轴线对齐,排出1号移液头211 内的废液至废液槽120;(此时2号移液头212在第四试剂槽134位置,第四试剂槽134装的是双蒸水,不会影响2号移液头212的洁净度);驱动推杆收缩,抵靠导向支架500的弹簧推动储液转盘100整体往下走,直到储液转盘100到达储液转盘100限位件530,完成储液转盘100的想下复位;8. Similar to step 6, turn the
9、类似步骤7,转动储液转盘100,使3#孔位与1号移液头211轴线对齐,吸取500uL的漂洗液PWE;(此时2号移液头212在第二避位孔142);9. Similar to step 7, turn the
10、重复步骤8;把漂洗后的PWE移至废液槽120;10. Repeat step 8; move the rinsed PWE to the
11、重复步骤9、步骤10;11. Repeat steps 9 and 10;
12、类似步骤7,转动储液转盘100,使第四试剂槽134与1号移液头211轴线对齐,吸取100uL的无RNA酶双蒸水至1#反应孔位;(此时2号移液头212在第三移液槽,因为第三移液槽位经过步骤9、步骤10、步骤11吸取后,已经空置,不会影响2号移液头212的洁净度);12. Similar to step 7, turn the
13、重复步骤8;把无RNA酶双蒸水移至废液槽120;13. Repeat step 8; move the RNase-free double distilled water to the
14、类似步骤7,转动储液转盘100,使第四试剂槽134与1号移液头211轴线对齐,吸取100uL的无RNA酶双蒸水至反应槽110位;核酸溶解于双蒸水之内,驱动震荡混匀,储液转盘100复位。14. Similar to step 7, turn the
15、在反应槽110位置加入一个磁环,让磁珠吸附到反应槽110的侧壁之上;15. Add a magnetic ring at the position of the
16、类似步骤7,转动储液转盘100,使反应槽110与2号移液头212轴线对齐,吸取5uL 的纯化的核酸(保持磁吸状态);(此时1号移液头211在第五避位孔145)16. Similar to step 7, turn the
17、类似步骤8,转动储液转盘100,使样本管孔150与2号移液头212轴线对齐,排出2号移液头212内的核酸至样本管孔150内的样本管400;(此时1号移液头211在避位2孔)17. Similar to step 8, rotate the
18、完成纯净的核酸转移到样本管400(反应管装有PCR反应液)。18. Complete the transfer of the pure nucleic acid to the sample tube 400 (the reaction tube is equipped with a PCR reaction solution).
三、取样。Three, sampling.
控制所述移液装置200将经前处理的样品溶液吸出,转移至样本管400内,将该样本管 400取出,如图12所示。具体如下所示:Control the
1、转动储液转盘100,使样本管孔150位与顶入机构330的轴线对齐,将储液转盘100 整体往上部推动,推动的同时,控制机械抓手抓取样本管400的管体420(PCR反应管),跟随储液转盘100往上推,步调一致,推动储液转盘100被密封盖600盖于储液转盘100转盘之上,此时储液转盘100在竖直方向的高度位置高于吸液时的位置;继续往上推动,推动管盖410盖于管体420(即PCR反应管)之上,完成PCR管的密封;继续往上推动储液转盘 100,但是抓取样本管400的机械抓手不再跟随储液转盘100往上,此时,样本管400已盖上盖子,且样本管400脱离卡盒本体,卡盒内部仍然是一个封闭结构,PCR管也是一个全封闭结构,两者的密封。1. Rotate the
2、外部驱动抓取卡盒本体丢弃;2. The external drive grabs the cartridge body and discards it;
3、机械抓手抓取样本管400(PCR反应管)至qPCR仪器上进行扩增检测,至此完成PCR 提取、扩增、检测。3. The mechanical gripper grabs the sample tube 400 (PCR reaction tube) and puts it on the qPCR instrument for amplification detection, thus completing PCR extraction, amplification, and detection.
上述分子诊断检测样本前处理过程,为全密封式处理过程,密封的实现包含两个步骤的密封,一、核酸提取及体系构建整个液体转移过程中在卡盒内部完成,实现密封;二、PCR 反应管从卡盒分离后,卡盒内部仍然是一个封闭结构,PCR管也是一个全封闭结构,实现密封。The pretreatment process of the above-mentioned molecular diagnostic test samples is a fully sealed process, and the realization of sealing includes two steps of sealing. 1. Nucleic acid extraction and system construction are completed inside the cartridge during the entire liquid transfer process to achieve sealing; 2. PCR After the reaction tube is separated from the cartridge, the interior of the cartridge is still a closed structure, and the PCR tube is also a fully closed structure to achieve sealing.
以上所述实施例的各技术特征可以进行任意的组合,为使描述简洁,未对上述实施例中的各个技术特征所有可能的组合都进行描述,然而,只要这些技术特征的组合不存在矛盾,都应当认为是本说明书记载的范围。The technical features of the above-mentioned embodiments can be combined arbitrarily. To make the description concise, all possible combinations of the technical features in the above-mentioned embodiments are not described. However, as long as there is no contradiction in the combination of these technical features, should be considered as within the scope of this specification.
以上所述实施例仅表达了本发明的几种实施方式,其描述较为具体和详细,但并不能因此而理解为对发明专利范围的限制。应当指出的是,对于本领域的普通技术人员来说,在不脱离本发明构思的前提下,还可以做出若干变形和改进,这些都属于本发明的保护范围。因此,本发明专利的保护范围应以所附权利要求为准。The above-mentioned embodiments only express several implementation modes of the present invention, and the descriptions thereof are relatively specific and detailed, but should not be construed as limiting the patent scope of the invention. It should be pointed out that those skilled in the art can make several modifications and improvements without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent for the present invention should be based on the appended claims.
Claims (20)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202221497556.2U CN218491744U (en) | 2022-06-15 | 2022-06-15 | Sample detection pretreatment equipment and reagent cartridges therein |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202221497556.2U CN218491744U (en) | 2022-06-15 | 2022-06-15 | Sample detection pretreatment equipment and reagent cartridges therein |
Publications (1)
Publication Number | Publication Date |
---|---|
CN218491744U true CN218491744U (en) | 2023-02-17 |
Family
ID=85181811
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202221497556.2U Active CN218491744U (en) | 2022-06-15 | 2022-06-15 | Sample detection pretreatment equipment and reagent cartridges therein |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN218491744U (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN117778172A (en) * | 2023-12-28 | 2024-03-29 | 江苏省淡水水产研究所 | Shrimp iridovirus PCR detection device |
-
2022
- 2022-06-15 CN CN202221497556.2U patent/CN218491744U/en active Active
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN117778172A (en) * | 2023-12-28 | 2024-03-29 | 江苏省淡水水产研究所 | Shrimp iridovirus PCR detection device |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN108660074B (en) | Integrated solution for nucleic acid extraction PCR amplification detection | |
WO2021254519A1 (en) | Sample processing and detection apparatus and application thereof | |
JP5890623B2 (en) | Liquid processing system and liquid processing method | |
CN104471369B (en) | Nucleic acid extraction tool set and nucleic acid-extracting apparatus | |
KR101964131B1 (en) | Closed extraction and purification apparatus | |
CN218491744U (en) | Sample detection pretreatment equipment and reagent cartridges therein | |
CN112760194B (en) | Closed simple RNA nucleic acid extraction device and preparation method and use method thereof | |
CN112094729B (en) | Rotary pipetting type totally-enclosed nucleic acid detection cartridge | |
CN117448118A (en) | Nucleic acid extraction and transformation integrated machine and application method thereof | |
CN111378574B (en) | Nucleic acid extraction and amplification device | |
CN105021835B (en) | A kind of Magnetic driving biological specimen processing meanss and its processing method | |
CN112852626A (en) | Integrated sample detection device and detection method thereof | |
CN116396850A (en) | Full-automatic nucleic acid sample processing system | |
CN217261445U (en) | Nucleic acid extraction equipment and reagent tube package subassembly and reagent tube subassembly | |
CN214916220U (en) | Liquid transfer mechanism for fluorescent PCR instrument | |
CN114907962A (en) | Sample testing pre-processing equipment and reagent cartridges therein | |
CN210314217U (en) | Automatic negative pressure purification system | |
CN109061212B (en) | A biochemical analyzer | |
CN218435735U (en) | Gas replacement device for liquid transfer device | |
US20040208790A1 (en) | Rotating and tapping apparatus | |
CN214422599U (en) | Rotary liquid-transferring type totally-enclosed nucleic acid detection card box | |
CN117000324A (en) | Pipetting element | |
EP4086333B1 (en) | Fully automatic gene analysis apparatus and gene analysis method | |
CN213348948U (en) | Reaction cup fixing mechanism and reaction cup holder | |
CN116144481A (en) | Automatic nucleic acid detection equipment |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20230926 Address after: Room 301, G10, South China new material innovation park, building 3, No. 31, Kefeng Road, Guangzhou hi tech Industrial Development Zone, Guangdong 510130 Patentee after: Guangzhou Weiyuan Medical Equipment Co.,Ltd. Patentee after: GUANGZHOU VISION GENE TECHNOLOGY Co.,Ltd. Patentee after: Guangzhou Weiyuan medical laboratory Co.,Ltd. Patentee after: Shenzhen Weiyuan Medical Technology Co.,Ltd. Patentee after: Beijing Weiyuan Medical Laboratory Co.,Ltd. Address before: Room 301, G10, South China new material innovation park, building 3, No. 31, Kefeng Road, Guangzhou hi tech Industrial Development Zone, Guangdong 510130 Patentee before: Guangzhou Weiyuan Medical Equipment Co.,Ltd. Patentee before: GUANGZHOU VISION GENE TECHNOLOGY Co.,Ltd. Patentee before: Guangzhou Weiyuan medical laboratory Co.,Ltd. Patentee before: Shenzhen Weiyuan Medical Technology Co.,Ltd. Patentee before: Weiyuan (Shenzhen) Medical Research Center Co.,Ltd. Patentee before: Nanjing Weiyuan Medical Laboratory Co.,Ltd. Patentee before: Beijing Weiyuan Medical Laboratory Co.,Ltd. |
|
TR01 | Transfer of patent right | ||
CP03 | Change of name, title or address |
Address after: Room 301, G10, South China new material innovation park, building 3, No. 31, Kefeng Road, Guangzhou hi tech Industrial Development Zone, Guangdong 510130 Patentee after: Guangzhou Weiyuan Medical Equipment Co.,Ltd. Country or region after: China Patentee after: GUANGZHOU VISION GENE TECHNOLOGY Co.,Ltd. Patentee after: Guangzhou Weiyuan medical laboratory Co.,Ltd. Patentee after: Weiyuan Medical Technology (Huzhou) Co.,Ltd. Patentee after: Beijing Weiyuan Medical Laboratory Co.,Ltd. Address before: Room 301, G10, South China new material innovation park, building 3, No. 31, Kefeng Road, Guangzhou hi tech Industrial Development Zone, Guangdong 510130 Patentee before: Guangzhou Weiyuan Medical Equipment Co.,Ltd. Country or region before: China Patentee before: GUANGZHOU VISION GENE TECHNOLOGY Co.,Ltd. Patentee before: Guangzhou Weiyuan medical laboratory Co.,Ltd. Patentee before: Shenzhen Weiyuan Medical Technology Co.,Ltd. Patentee before: Beijing Weiyuan Medical Laboratory Co.,Ltd. |
|
CP03 | Change of name, title or address |