CN103114035B - Device for fully-enclosed extraction, transfer and storage of nucleic acids - Google Patents
Device for fully-enclosed extraction, transfer and storage of nucleic acids Download PDFInfo
- Publication number
- CN103114035B CN103114035B CN201310080221.XA CN201310080221A CN103114035B CN 103114035 B CN103114035 B CN 103114035B CN 201310080221 A CN201310080221 A CN 201310080221A CN 103114035 B CN103114035 B CN 103114035B
- Authority
- CN
- China
- Prior art keywords
- rifle
- sleeve
- suction nozzle
- reagent
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Automatic Analysis And Handling Materials Therefor (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a device for fully-enclosed extraction, transfer and storage of nucleic acids. The device comprises a sucker and a reaction tube, wherein the sucker comprises a lance pointed end, an O-shaped sealing ring, a spring, a sleeve and a sucker insulated plug, wherein the lance pointed end comprises a lance pointed pipe and a lance pointed needle, wherein an annular groove is formed in the upper part of the inner wall of the sleeve, an annular baffle plate is arranged on the lower part of the inner wall of the sleeve, the lance pointed end is arranged in a cavity of the sleeve, the O-shaped sealing ring is embedded between the lance pointed pipe of the lance pointed head and the sleeve, the spring is sheathed on the outer side of the lance pointed needle, the sucker insulated plug is fixedly connected at the bottom of the cavity of the sleeve, the reaction tube comprises a reagent insulated plug and a reagent storage box and is in a sealing state, and the reagent insulated plug of the reaction tube is in contact with the sucker insulated plug of the sucker. The device can solve the problem that high-concentration aerosol is easy to escape in a process of absorbing nucleic acid amplification afterproducts, thus resulting in the cross contamination.
Description
Technical field
The present invention relates to the medical devices of molecular diagnosis, specifically, relate to the device of a kind of totally-enclosed extraction, transfer and storage nucleic acid.
Background technology
Nucleic acid amplification is the conventional means of molecular diagnosis, the effect by enzyme and primer in reaction tube of the nucleic acid molecules of sample to be tested can be index and doubly increase, amplify thus thing to be detected for follow-up signal-obtaining experiment, yet the nucleic acid molecules that the drawback of nucleic acid amplification is high concentration easily exists with aerocolloidal form, once open reaction tube lid, make aerosol loss can cause the cross pollution of sample room in air, produce false-positive result.All molecular diagnosis products that relate to nucleic acid amplification technologies all adopt 2 kinds of modes to prevent this cross pollution at present, (1) adopt covered signal interpretation mode, as utilize the mode of real-time quantitative fluorescence PCR, (2) to adopt the mode of totally-enclosed cartridge type will move liquid, nucleic acid amplification and signal interpretation and carry out in a reaction cartridge; Yet and shortcoming also clearly (1) be limited by can not testing signal of uncapping, detection method is single, (2) totally-enclosed cartridge consumptive material has increased cost greatly, is unfavorable for promoting.Therefore both how to acquire the detection of can (reaction tube) uncapping, the molecular diagnosis new approaches of not cross pollution seem particularly urgent again.
Summary of the invention
technical problem:technical problem to be solved by this invention is: the device of a kind of totally-enclosed extraction, transfer and storage nucleic acid is provided, to solve to uncap, draws in the process of nucleic acid amplification afterproduct, high concentration aerosol is easily overflowed, and causes the problem of cross pollution.
technical scheme:for solving the problems of the technologies described above, the technical solution used in the present invention is as follows:
The device of a kind of totally-enclosed extraction, transfer and storage nucleic acid, this device comprises suction nozzle and reaction tube, described suction nozzle comprises rifle tip, O RunddichtringO, spring, sleeve and suction nozzle batching pig, rifle tip comprises rifle point pipe and is fixedly connected on the rifle prong of rifle point pipe bottom surface, the bottom of rifle prong is most advanced and sophisticated, in rifle point pipe and rifle prong, be provided with the cavity communicating, the top of sleeve lining is provided with annular groove, the bottom of sleeve lining is provided with ring baffle, the internal diameter of ring baffle is less than the external diameter of rifle point pipe, and the internal diameter of ring baffle is greater than the external diameter of rifle prong; Rifle tip is arranged in the inner chamber of sleeve, and O RunddichtringO is embedded between the rifle point pipe and sleeve of rifle tip, and O RunddichtringO is arranged in annular groove; Spring housing is contained in rifle prong outside, and the top of spring offsets with the bottom surface of rifle point pipe, and bottom and the ring baffle of spring offset, and suction nozzle batching pig is fixedly connected on the bottom of barrel bore, and suction nozzle batching pig is positioned at below ring baffle;
Described reaction tube comprises the reagent storage box that reagent batching pig and top are opening, reagent batching pig is fixedly connected on the opening of reagent storage box, reaction tube is sealing state, and reaction tube is positioned at suction nozzle below, and the reagent batching pig of reaction tube and the suction nozzle batching pig of suction nozzle touch mutually.
Further, described rifle point pipe, rifle prong, O RunddichtringO, sleeve, ring baffle coaxially arranges.
Further, described rifle point pipe is made by PP or PE material, and rifle prong is made by stainless steel.
Further, the outer surface of described O RunddichtringO scribbles medical ventolin coating.
beneficial effect:compared with prior art, technical scheme of the present invention has following beneficial effect:
(1) full-enclosed structure design, effectively prevents the pollution of nucleic acid in transfer process.Compare with traditional suction pipette head, device of the present invention is to improve on the basis of traditional suction pipette head, and has increased a rifle point locking device, and sealing device comprises O RunddichtringO, spring, sleeve and suction nozzle batching pig.O RunddichtringO is embedded between the rifle point pipe and sleeve of rifle tip, and O RunddichtringO is arranged in the annular groove of sleeve.O RunddichtringO has been isolated inner chamber and the external environment of sleeve.Suction nozzle batching pig is fixedly connected on the bottom of barrel bore.Suction nozzle batching pig also plays sealing function, has isolated inner chamber and the external environment of sleeve.Like this, by suction nozzle batching pig and O RunddichtringO are set, sealed sleeve, thereby the rifle prong that is arranged in sleeve is also isolated from the outside.In use, suction nozzle batching pig is positioned on reagent batching pig, and both contact.Like this, rifle prong, through after suction nozzle batching pig, directly pierces through reagent batching pig, enters in reagent storage box, can not contact with the external world.This suction nozzle and reaction tube have been combined to form an enclosed environment, have prevented the amplified production aerosol effusion in reaction tube, have also eliminated cross pollution, have improved the accuracy of Bioexperiment and medical diagnosis on disease.Like this, device of the present invention can guarantee suction pipette head in imbibition, move in the process of liquid and liquid feeding, reagent can not contact with extraneous air and produce cross pollution.
(2) applicability is strong, and suction nozzle can coordinate current conventional pipettor manually or automatically, goes forward side by side to exercise and uses.
(3) simple in structure, low cost of manufacture.The inventive system comprises suction nozzle and reaction tube, suction nozzle comprises rifle tip, O RunddichtringO, spring, sleeve and suction nozzle batching pig, and reaction tube comprises reagent batching pig and reagent storage box.Whole apparatus structure is simple.The material of simultaneously making this device is all conventional medical material, low cost of manufacture, and difficulty of processing is low, is easy to assembling.
Accompanying drawing explanation
Fig. 1 is the structural representation that rifle tip of the present invention is arranged in sleeve.
Structural representation when Fig. 2 is rifle tip insertion reaction tube of the present invention.
Fig. 3 is the structural representation of suction nozzle of the present invention.
Fig. 4 is the structural representation of reaction tube of the present invention.
In figure, have: rifle tip 1, rifle point pipe 101, rifle prong 102, O RunddichtringO 2, spring 3, sleeve 4, suction nozzle batching pig 5, reagent batching pig 6, reagent storage box 7, ring baffle 8.
The specific embodiment
Below in conjunction with accompanying drawing, technical scheme of the present invention is described in detail.
As shown in Figures 1 to 4, the device of a kind of totally-enclosed extraction of the present invention, transfer and storage nucleic acid comprises suction nozzle and reaction tube.Suction nozzle comprises rifle tip 1, O RunddichtringO 2, spring 3, sleeve 4 and suction nozzle batching pig 5.Rifle tip 1 comprises rifle point pipe 101 and is fixedly connected on the rifle prong 102 of rifle point pipe 101 bottom surfaces.Rifle tip adopts injection molding to form.As preferably, sleeve 4 by the English full name of PP(is: Polypropylene, Chinese translation: polypropylene, in literary composition, be called for short PP) or the English full name of PE(be: Polyethylene, Chinese translation: polyethylene, in literary composition, being called for short PE) material makes, and rifle point pipe 101 is made by PP or PE material, and rifle prong 102 is made by stainless steel.The bottom of rifle prong 102 is most advanced and sophisticated.Like this, rifle prong 102 is easily poked suction nozzle batching pig 5 and reagent batching pig 6.In rifle point pipe 101 and rifle prong 102, be provided with the cavity communicating.The top of sleeve 4 inwalls is provided with annular groove, and the bottom of sleeve 4 inwalls is provided with ring baffle 8.The internal diameter of ring baffle 8 is less than the external diameter of rifle point pipe 101, and the internal diameter of ring baffle 8 is greater than the external diameter of rifle prong 102.Like this, rifle prong 102 can be through the inner chamber of ring baffle 8, and rifle point pipe 101 can not be through the inner chamber of ring baffle 8.Rifle tip 1 is arranged in the inner chamber of sleeve 4.O RunddichtringO 2 is embedded between the rifle point pipe 101 and sleeve 4 of rifle tip, and O RunddichtringO 2 is arranged in annular groove.O RunddichtringO 2 plays sealing function, has isolated inner chamber and the external environment of sleeve 4.Spring 3 is sleeved on rifle prong 102 outsides, and the bottom surface of the top of spring 3 and rifle point pipe 101 offsets, and the bottom of spring 3 and ring baffle 8 offset.Suction nozzle batching pig 5 is fixedly connected on the bottom of sleeve 4 inner chambers, and suction nozzle batching pig 5 is positioned at ring baffle 8 belows.Suction nozzle batching pig 5 also plays sealing function, has isolated inner chamber and the external environment of sleeve 4.Reaction tube comprises the reagent storage box 7 that reagent batching pig 6 and top are opening.Reagent batching pig 6 is fixedly connected on the opening of reagent storage box 7, and reagent batching pig 6 has sealed reagent storage box 7, and making reaction tube is sealing state.Reagent batching pig 6 shapes are preferably patty.Reagent batching pig 6 is placed on the upper opening of reagent storage box 7, prevents that in nucleic acid amplification reaction pipe, aerosol spills in external environment condition.Reaction tube is positioned at suction nozzle below, and the reagent batching pig 6 of reaction tube and the suction nozzle batching pig 5 of suction nozzle touch mutually.
Further, described rifle point pipe 101, rifle prong 102, O RunddichtringO 2, sleeve 4, ring baffle 8 coaxially arranges.Be conducive to like this rifle tip 1 draws reagent and add reagent in reagent storage box 7 from reagent storage box 7.
Further, the outer surface of described O RunddichtringO 2 scribbles medical ventolin coating.This coating can play effect lubricated and sealing.
Further, as preferred version, described suction nozzle batching pig 5 is made by medical grade rubber or medical natural rubber.Described reagent batching pig 6 is made by medical grade rubber or medical natural rubber.Medical grade rubber or medical natural rubber material have good springiness, the feature easily piercing through.Reagent storage box 7 is made by PP or PE material.Reagent storage box 7 is preferably single hose, or the reaction of 96 holes is board-like.
The device installation process of the totally-enclosed extraction of said structure, transfer and storage nucleic acid is: on O RunddichtringO 2 surfaces, smear after medical ventolin coating, O RunddichtringO 2 is positioned in the annular groove on sleeve 4 inwalls, spring 3 is packed in sleeve 4 by sleeve 4 tops, again rifle tip 1 is positioned in sleeve 4, make spring 3 be sleeved on rifle prong 102 outsides, and make spring 3 between the bottom surface and ring baffle 8 of rifle point pipe 101, the lower end of sleeve 4 seals with suction nozzle batching pig 5, thereby completes the assembling of suction nozzle.With reagent batching pig 6, the opening of reagent storage box 7 is sealed, complete the assembling of reaction tube.
The course of work of the device of the totally-enclosed extraction of said structure, transfer and storage nucleic acid is: when imbibition or liquid feeding, suction nozzle is installed on pipettor, and the lower end of suction nozzle is vertical, and the upper port of aiming at reaction tube; Pipettor is when pressing down, make rifle prong 102 penetrate suction nozzle batching pig 5 and reagent batching pig 6 downwards, enter in closed nucleic acid amplification reaction pipe, can carry out imbibition or liquid feeding to the reagent in closed nucleic acid amplification reaction pipe, then upwards promote rifle tip 1, the pressure being subject on spring 3 discharges gradually, rifle prong 102 is not being got back to sleeve 4 when interior completely, owing to being subject to the compressing of spring 3, suction nozzle batching pig 5 and reagent batching pig 6 be close contact always, until rifle prong 102 returns in sleeve 4 completely.When rifle prong 102 returns in sleeve 4 completely, spring 3 does not have pressure, and sleeve 4 will move upward with rifle tip 1, and sleeve 4 is separated with the enclosed nucleic acid amplification reaction pipe of bottom.Because reagent batching pig 6 and suction nozzle batching pig 5 all adopt medical grade rubber or medical natural rubber to make, so after rifle prong 102 is extracted, the reagent batching pig 6 that rifle prong 102 passes and the hole of suction nozzle batching pig 5 will be automatically closed, and sealed reagent storage box 7 and sleeve 4, avoid reagent to leak.Adopt this device in whole imbibition, move in liquid and liquid feeding process, can avoid reagent to contact with outside air, prevented cross pollution.
Lift a specific embodiment below, be described below:
To pressing down rifle tip 1, rifle prong 102 penetrates after suction nozzle batching pig 5 and reagent batching pig 6, is deep in reagent storage box 7, realizes imbibition and liquid feeding action.After having moved, upwards promote rifle tip 1, until rifle prong 102, get back to sleeve 4 completely when interior, while continuing to promote rifle tip 1, when spring 3 is not under pressure, sleeve 4 will move upward with rifle tip 1, separated with the enclosed nucleic acid amplification reaction pipe of bottom.In whole imbibition, move in liquid and liquid feeding process, the reagent in rifle prong 102 and reaction tube can not be exposed in external environment condition.
Claims (9)
1. a totally-enclosed extraction, shift and store the device of nucleic acid, it is characterized in that, this device comprises suction nozzle and reaction tube, described suction nozzle comprises rifle tip (1), O RunddichtringO (2), spring (3), sleeve (4) and suction nozzle batching pig (5), rifle tip (1) comprises rifle point pipe (101) and is fixedly connected on the rifle prong (102) of rifle point pipe (101) bottom surface, the bottom of rifle prong (102) is most advanced and sophisticated, in rifle point pipe (101) and rifle prong (102), be provided with the cavity communicating, the top of sleeve (4) inwall is provided with annular groove, the bottom of sleeve (4) inwall is provided with ring baffle (8), the internal diameter of ring baffle (8) is less than the external diameter of rifle point pipe (101), the internal diameter of ring baffle (8) is greater than the external diameter of rifle prong (102), rifle tip (1) is arranged in the inner chamber of sleeve (4), and O RunddichtringO (2) is embedded between the rifle point pipe (101) and sleeve (4) of rifle tip, and O RunddichtringO (2) is arranged in annular groove, spring (3) is sleeved on rifle prong (102) outside, and the bottom surface of the top of spring (3) and rifle point pipe (101) offsets, the bottom of spring (3) and ring baffle (8) offset, suction nozzle batching pig (5) is fixedly connected on the bottom of sleeve (4) inner chamber, and suction nozzle batching pig (5) is positioned at ring baffle (8) below,
Described reaction tube comprises the reagent storage box (7) that reagent batching pig (6) and top are opening, reagent batching pig (6) is fixedly connected on the opening of reagent storage box (7), reaction tube is sealing state, reaction tube is positioned at suction nozzle below, and the reagent batching pig (6) of reaction tube and the suction nozzle batching pig (5) of suction nozzle tactile mutually.
2. according to the device of totally-enclosed extraction claimed in claim 1, transfer and storage nucleic acid, it is characterized in that, described rifle point pipe (101), rifle prong (102), O RunddichtringO (2), sleeve (4), ring baffle (8) coaxially arrange.
3. the device of totally-enclosed extraction according to claim 1, transfer and storage nucleic acid, is characterized in that, described rifle point pipe (101) is made by PP or PE material, and rifle prong (102) is made by stainless steel.
4. the device of totally-enclosed extraction according to claim 1, transfer and storage nucleic acid, is characterized in that, the outer surface of described O RunddichtringO (2) scribbles medical ventolin coating.
5. the device of totally-enclosed extraction according to claim 1, transfer and storage nucleic acid, is characterized in that, described sleeve (4) is made by PP or PE material.
6. the device of totally-enclosed extraction according to claim 1, transfer and storage nucleic acid, is characterized in that, described suction nozzle batching pig (5) is made by medical grade rubber.
7. the device of totally-enclosed extraction according to claim 1, transfer and storage nucleic acid, is characterized in that, described reagent batching pig (6) is made by medical grade rubber.
8. the device of totally-enclosed extraction according to claim 1, transfer and storage nucleic acid, is characterized in that, described reagent storage box (7) is made by PP or PE material.
9. the device of totally-enclosed extraction according to claim 1, transfer and storage nucleic acid, is characterized in that, described reagent storage box (7) is that single hose or the reaction of 96 holes are board-like.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310080221.XA CN103114035B (en) | 2013-03-14 | 2013-03-14 | Device for fully-enclosed extraction, transfer and storage of nucleic acids |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310080221.XA CN103114035B (en) | 2013-03-14 | 2013-03-14 | Device for fully-enclosed extraction, transfer and storage of nucleic acids |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103114035A CN103114035A (en) | 2013-05-22 |
| CN103114035B true CN103114035B (en) | 2014-04-02 |
Family
ID=48412453
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310080221.XA Active CN103114035B (en) | 2013-03-14 | 2013-03-14 | Device for fully-enclosed extraction, transfer and storage of nucleic acids |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103114035B (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015192330A1 (en) * | 2014-06-17 | 2015-12-23 | 深圳迈瑞生物医疗电子股份有限公司 | Nucleic acid extraction device and method |
| CN105039135B (en) * | 2015-06-29 | 2017-01-25 | 诸暨脉达生物科技有限公司 | Semiautomatic paraffin pre-sealing and sample-adding device and using method thereof |
| CN106318865B (en) * | 2016-10-25 | 2018-03-16 | 西安交通大学 | A kind of nucleic acid extraction and the portable unit of gene magnification |
| CN106434639B (en) * | 2016-12-01 | 2018-07-20 | 山东森芃生物科技有限公司 | A kind of DNA extraction method without tube |
| CN108531377A (en) * | 2018-06-26 | 2018-09-14 | 广东省第二人民医院（广东省卫生应急医院） | It is a kind of can pre-filled reagent diaphragm type nucleic acid amplification airtight reactor tube |
| CN109856413A (en) * | 2019-03-29 | 2019-06-07 | 广东合鑫生物科技有限公司 | A kind of reagent pipetting volume device and reagent pipetting volume system |
| CN110102242B (en) * | 2019-06-12 | 2023-09-05 | 成都恩普生医疗科技有限公司 | Reaction cup integrating packaging and mixing method |
| CN114058472A (en) * | 2020-07-30 | 2022-02-18 | 苏州中加康美科技有限公司 | Nucleic acid reagent reaction unit |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2566257Y (en) * | 2002-09-09 | 2003-08-13 | 黄一平 | Anti-pollution suction head for micro liquid suction device |
| CN1745305A (en) * | 2003-01-31 | 2006-03-08 | 环球生物研究株式会社 | Monitoring function-equipped dispensing system and method of monitoring dispensing device |
| CN100577295C (en) * | 2001-02-09 | 2010-01-06 | 拜奥希特公司 | Method for adjusting distribution volume in sucker and sucker for distributing liquid |
| CN102965271A (en) * | 2012-11-07 | 2013-03-13 | 苏州市外来有害生物防控技术中心 | Device for quickly extracting pine wood nematode nucleic acid |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2003251996A1 (en) * | 2002-07-23 | 2004-02-09 | Protedyne Corporation | Liquid handling tool having hollow plunger |
| JP5363604B2 (en) * | 2012-03-21 | 2013-12-11 | 株式会社日立ハイテクノロジーズ | Chemical analyzer |
-
2013
- 2013-03-14 CN CN201310080221.XA patent/CN103114035B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100577295C (en) * | 2001-02-09 | 2010-01-06 | 拜奥希特公司 | Method for adjusting distribution volume in sucker and sucker for distributing liquid |
| CN2566257Y (en) * | 2002-09-09 | 2003-08-13 | 黄一平 | Anti-pollution suction head for micro liquid suction device |
| CN1745305A (en) * | 2003-01-31 | 2006-03-08 | 环球生物研究株式会社 | Monitoring function-equipped dispensing system and method of monitoring dispensing device |
| CN102965271A (en) * | 2012-11-07 | 2013-03-13 | 苏州市外来有害生物防控技术中心 | Device for quickly extracting pine wood nematode nucleic acid |
Non-Patent Citations (1)
| Title |
|---|
| JP特开2012-127974A 2012.07.05 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103114035A (en) | 2013-05-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103114035B (en) | Device for fully-enclosed extraction, transfer and storage of nucleic acids | |
| CN204439557U (en) | Tubular type test paper | |
| CN201658978U (en) | Bioexperiment device | |
| CN102634448B (en) | Biological sampling swab | |
| CN105586256B (en) | A kind of closed pathogen detection cartridge | |
| CN205382165U (en) | Closed pathogen detection card box | |
| CN203715632U (en) | Pcr reaction tube | |
| CN216764896U (en) | Nucleic acid extraction, constant temperature amplification and signal reading integrated sealed casing device | |
| CN102809527B (en) | Intubation tube type splash-proof vacuum blood sedimentation tube | |
| CN214142375U (en) | Nucleic acid reagent reaction unit | |
| CN204649474U (en) | A kind of microbiological analysis sampler | |
| CN206489076U (en) | A kind of Novel ATP fluoroscopic examination swab | |
| CN201368820Y (en) | Injector for cell examination | |
| CN104819870A (en) | Microorganism analytical sampling device | |
| CN220450197U (en) | Detection consumable and detection equipment | |
| CN218381835U (en) | Suction head with puncture function | |
| CN217809346U (en) | PCR tube cap | |
| CN204503029U (en) | A kind of Miniature device for gas experiment | |
| CN203949896U (en) | The automatic sampler of coulometry micro-water analyzer and injector sampling assemble | |
| CN204613187U (en) | A kind of Determination of Organic Acids chemical detection instrument | |
| CN204702755U (en) | A kind of sliding type closes pathogen detection cartridge | |
| CN203953680U (en) | A kind of novel evacuated blood taking tube | |
| CN209113897U (en) | Bacteria Incubator | |
| CN220618932U (en) | Integrated reaction tube for fluorescence method nucleic acid detection | |
| CN201819886U (en) | Vacuum self-suction food safety rapid detection pipe |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |