CN214142375U

CN214142375U - Nucleic acid reagent reaction unit

Info

Publication number: CN214142375U
Application number: CN202022093554.4U
Authority: CN
Inventors: 张金泉
Original assignee: Suzhou Zhongjia Kangmei Technology Co ltd
Current assignee: Suzhou kangjianyun Medical Technology Co.,Ltd.
Priority date: 2020-09-22
Filing date: 2020-09-22
Publication date: 2021-09-07
Anticipated expiration: 2030-09-22

Abstract

The utility model discloses a nucleic acid reagent reaction device, which comprises a sample transfer unit, a vacuum sealed reaction unit and a vacuum sealed sample storage unit; the sample transfer unit comprises an accommodating tube, a liquid suction/discharge module, a sample introduction module and a sample adding module, wherein the liquid suction/discharge module, the sample introduction module and the sample adding module are communicated with the accommodating tube; valves are arranged on the communicating pipes of the sample introduction module, the sample adding module and the accommodating pipe; the sample introduction module extends into the sample storage unit and is connected with the sample storage unit in a sealing way; the sample adding module extends into the reaction unit and is connected with the reaction unit in a sealing way; the liquid suction/discharge module is used for sucking the sample in the sample storage unit into the accommodating pipe and discharging the sample in the accommodating pipe to the reaction unit. The utility model provides a difficult problem that carries out the nucleic acid short-term test on scene under non-laboratory environment, can the multi-scene use, to nucleic acid detection measure the clothing antedisplacement to clinical, to the basic unit, be one need not laboratory environment, detection time is shorter, the detection means is more convenient, the rate of accuracy is higher, the better nucleic acid detection iteration technique of security.

Description

Nucleic acid reagent reaction unit

Technical Field

The application relates to a nucleic acid reagent reaction device, and belongs to the technical field of nucleic acid detection.

Background

The polymerase chain reaction (hereinafter referred to as PCR) technique is a rapid in vitro amplificationTechniques for amplifying DNA, which generally include a plurality of cycles, each cycle including three steps of denaturation, annealing, and extension, and ideally, the number of nucleic acid molecules of interest is doubled for each cycle. Generally, after 30-40 cycles, the number of nucleic acid molecules of interest can be amplified to approximately 10⁹And (4) doubling. Thus, PCR is the most efficient method for obtaining DNA fragments of interest in large quantities in vitro, and the obtained nucleic acid molecules can be used for further analysis and testing.

However, the high sensitivity of PCR is always accompanied by a drawback: are extremely prone to contamination. Contamination with only 1 copy of DNA may result in erroneous detection results. Therefore, in order to avoid sample contamination during detection, nucleic acid detection in the prior art must be performed in a laboratory environment.

In the prior art, nucleic acid detection in a laboratory environment needs expensive reagents or precise instruments, is complex to operate and is not suitable for on-site rapid detection of nucleic acid.

SUMMERY OF THE UTILITY MODEL

The present application aims to provide a nucleic acid reagent reaction device to solve the technical problem that the nucleic acid reaction device in the prior art is not suitable for the on-site rapid detection of nucleic acid.

The utility model discloses a nucleic acid reagent reaction unit, include: the device comprises a sample transfer unit, a vacuum-sealed reaction unit and a vacuum-sealed sample storage unit;

the sample transfer unit comprises an accommodating tube, a liquid suction/discharge module, a sample introduction module and a sample adding module, wherein the liquid suction/discharge module, the sample introduction module and the sample adding module are communicated with the accommodating tube; valves are arranged on the communicating pipelines of the sample feeding module, the sample feeding module and the accommodating tube, and the valves are used for controlling the accommodating tube to be communicated with only one of the sample feeding module and the sample feeding module at the same time;

the sample introduction module extends into the sample storage unit and is connected with the sample storage unit in a sealing way;

the sample adding module extends into the reaction unit and is connected with the reaction unit in a sealing way;

the liquid suction/discharge module is used for sucking the sample in the sample storage unit into the accommodating pipe and discharging the sample in the accommodating pipe to the reaction unit.

Preferably, the sample introduction module comprises a sample introduction needle, a sample introduction pipe, a first sample introduction connecting piece and a first sealing ring sleeved outside the first sample introduction connecting piece;

one end of the sample injection tube is communicated with the accommodating tube, and the other end of the sample injection tube is communicated with the sample injection needle;

the sample injection needle is fixedly connected with the first sample injection connecting piece and is used for extending into the sample storage unit and transmitting the sample in the sample storage unit into the accommodating pipe through the sample injection pipe;

the first sample introduction connecting piece is connected with the sample storage unit in a sealing way through the first sealing ring

Preferably, the sample storage unit comprises a vacuum-sealed cracking cup and a second sample introduction connecting piece which is arranged on the periphery of a cup opening of the cracking cup for a circle and is connected with the cup opening of the cracking cup; the inner diameter of the second sample introduction connecting piece is larger than that of the cracking cup;

the first sample introduction connecting piece comprises a first connecting section and a second connecting section which are connected with each other;

the first sealing ring is sleeved at the joint of the first connecting section and the second connecting section;

the outer diameter of the first connecting section is matched with the inner diameter of the second sampling connecting piece;

the outer diameter of the second connecting section is matched with the inner diameter of the cracking cup.

Preferably, the clamping device further comprises a first clamping component;

the first clamping assembly comprises a first clamping pin and a first clamping groove matched with the first clamping pin;

the first clamping pin is arranged on the outer wall of the first connecting section;

the first clamping groove is arranged on the side wall of the second sample injection connecting piece, and the setting position of the first clamping groove corresponds to the setting position of the first clamping pin.

Preferably, the sample adding module comprises a sample adding head, a sample adding pipe, a first sample adding connecting piece and a second sealing ring sleeved outside the first sample adding connecting piece;

one end of the sample adding pipe is communicated with the accommodating pipe, and the other end of the sample adding pipe is communicated with the sample adding head;

the sample adding head is fixedly connected with the first sample adding connecting piece and is used for extending into the reaction unit and transmitting the sample in the accommodating tube into the reaction unit through the sample adding tube;

the first sample adding connecting piece is connected with the reaction unit in a sealing mode through the second sealing ring. Preferably, the first sample application connector comprises a third connecting segment and a fourth connecting segment which are connected with each other;

the outer diameter of the fourth connecting section is smaller than that of the third connecting section and is matched with the inner diameter of the reaction unit;

the second sealing ring is sleeved at the joint of the third connecting section and the fourth connecting section;

the first sample adding connecting piece is connected with the reaction unit in a sealing mode through the second sealing ring.

Preferably, the reaction unit comprises a vacuum chamber, a vacuum-sealed reaction tube and a second sample adding connector;

the orifice of the reaction tube is arranged in the vacuum cabin, the inner diameter of the reaction tube is larger than the maximum outer diameter of the sample adding head, and a reaction reagent is arranged in the reaction tube;

the second sample adding connecting piece is arranged on the periphery of the hatch of the vacuum cabin and is connected with the hatch of the vacuum cabin; the inner diameter of the second sample adding connecting piece is matched with the outer diameter of the third connecting section;

the inner diameter of the vacuum chamber is matched with the outer diameter of the fourth connecting section;

the sample adding head sequentially penetrates through the second sample adding connecting piece, the vacuum cabin and the reaction tube, and the sample in the accommodating tube is transmitted into the reaction tube through the sample adding tube.

Preferably, the sample adding module further comprises a third sealing ring;

the third sealing ring is sleeved outside the sample adding head and is positioned at one end close to the first sample adding connecting piece;

the reaction tube is connected with the first sample adding connecting piece in a sealing way through the third sealing ring.

Preferably, the clamping device further comprises a second clamping component;

the second clamping component comprises a second clamping pin and a second clamping groove matched with the second clamping pin;

the second bayonet is arranged on the outer wall of the third connecting section;

the second clamping groove is arranged on the side wall of the second sample adding connecting piece, and the setting position of the second clamping groove corresponds to the setting position of the second clamping pin.

Preferably, the pipetting/tapping module comprises: the piston and a push-pull rod connected with the piston;

the piston is arranged in the accommodating pipe;

the push-pull rod extends out of the accommodating pipe;

preferably, the number of the sample adding heads is 1 or more; the number of the reaction tubes corresponds to that of the sample adding heads;

preferably, the shape of the sample addition head is conical;

preferably, the valve comprises a first one-way valve and a second one-way valve;

the first one-way valve is arranged on a communication pipeline between the sample introduction module and the accommodating pipe and is used for controlling the sample to enter the accommodating pipe;

the second one-way valve is arranged on a communication pipeline between the sample adding module and the accommodating pipe and is used for controlling the sample to be discharged from the accommodating pipe;

preferably, the valve core of the valve is cylindrical, and a first through hole and a second through hole are sequentially arranged along the axial direction of the valve core;

the plane where the hole opening of the first through hole is located is perpendicular to the plane where the hole opening of the second through hole is located;

the first through hole is used for communicating the sample introduction module and the accommodating tube;

the second through hole is used for communicating the sample adding module and the accommodating tube.

The utility model discloses a nucleic acid reagent reaction unit compares in prior art, has following beneficial effect:

the utility model discloses a nucleic acid reagent reaction unit has solved the difficult problem that carries out the nucleic acid short-term test under non-laboratory environment on the scene, can the multi-scene use, to nucleic acid detection measure body tailor antedisplacement to clinical, to the basic unit, be one need not laboratory environment, detection time shorter, the detection means is more convenient, the rate of accuracy is higher, the better nucleic acid detection iteration technique of security.

The utility model discloses a nucleic acid reagent reaction unit, from the schizolysis to the sample and shift the sample to the whole flow of reaction unit, the sample all is in vacuum environment, so, need not the laboratory environment, easy operation is convenient.

The utility model discloses a store up appearance unit and advance the junction of appearance unit and set up to the echelonment structure to set up the sealing washer at the connection position, thereby make advance the appearance unit and stretching into the in-process of storing up the appearance unit, can realize fast storing up the appearance unit and advance the sealed between the appearance unit, avoid the pollution that the external air caused. In the process, the sample injection needle can rapidly extend into the vacuum-sealed cracking cup, and a sample cracked by using the cracking liquid is extracted from the cracking cup.

The utility model discloses a guarantee to store up appearance unit and advance kind of block connection's steadiness, store up appearance unit and advance kind of unit separation when avoiding taking place to oscillate, cause the external air pollution sample, still set up first joint subassembly, realize joint between them.

The utility model discloses a sealed and vacuum chamber and the vacuum seal's of first application of sample connecting piece and second application of sample connecting piece reaction tube, realized tertiary sealed, when preventing the product of amplification to leak, reduced external internal pollution, guarantee that nucleic acid amplification and testing process are totally closed, can prevent effectively that the product of nucleic acid amplification from leaking, avoid cross contamination to can effectively avoid the pollution in nucleic acid amplification and the testing process, the device has played the effect in nucleic acid laboratory.

The number of the reaction tubes and the number of the sample adding heads of the utility model are corresponding, and can be a plurality; when the number of the nucleic acid amplification products is multiple, multiple types of nucleic acid amplification detection can be carried out simultaneously, and mutual influence is avoided, so that the detection efficiency is greatly improved.

The utility model discloses a guarantee reaction unit and application of sample unit connection's steadiness, reaction unit and application of sample unit separation when avoiding taking place to oscillate cause the external air to pollute the sample, have still set up second joint subassembly, realize joint between them.

The utility model discloses a realize that the sample injector shifts the sample in the schizolysis cup to the reaction tube more conveniently in, set up the piston and with the push-and-pull rod of piston connection for inhale the holding pipe with the sample in the schizolysis cup and discharge the intraductal sample of holding to the reaction tube. Meanwhile, in order to obtain the amount of the sample, a scale mark or a quantitative limit point is arranged on the shell opposite to the push-pull rod.

The utility model discloses a realize the miniaturation and move the liquid to the liquid measure is moved in the accurate control, and the diameter of injecing the pinhole of the application of sample needle in the application of sample head and the pinhole of appearance needle is between 0.3-0.7 mm.

Because the interior of the cracking cup is a vacuum sealing environment, if a sample needs to be extracted from the cracking cup, the sample injection needle needs to be extended into the cracking cup, so that the mouth of the vacuum cracking cup is sealed by using sealing paper, and at the moment, the sample injection needle can penetrate through the sealing paper to suck the sample from the cracking cup; similarly, the sample adding head needs to pass through the vacuum chamber and the vacuum-sealed reaction tube to send the sample into the reaction tube, so that the opening of the vacuum reaction tube is also sealed by the sealing paper, and the hatch of the vacuum chamber corresponding to the opening of the reaction tube is also sealed by the sealing paper, so that the sample adding head can add the sample into the reaction tube through the sealing paper. Because the application of sample head need wear two-layer sealing paper, for reducing the resistance to for avoiding the damage to syringe needle in it, the utility model discloses set up the application of sample head into the cone.

For further guarantee sample and the reaction reagent in the reaction tube work under the interference of no external environment, the utility model discloses outer wall a week of keeping away from most advanced one end at the application of sample head is provided with the third sealing washer, is used for sealed sealing the reaction tube with space between the first application of sample connecting piece.

The sample transfer unit, the reaction unit and the sample storage unit are clamped and fixed, and after detection is finished, the three are always in a sealing state, can be directly recovered as medical wastes, and cannot generate pollution.

Drawings

FIG. 1 is a schematic view showing a structure of a sample transfer unit in a nucleic acid reagent reaction apparatus according to a first embodiment of the present invention;

FIG. 2 is a schematic view showing the structure of a reaction unit in a nucleic acid reagent reaction apparatus according to a first embodiment of the present invention;

FIG. 3 is a schematic view showing a sample storage unit in a nucleic acid reagent reaction apparatus according to a first embodiment of the present invention;

FIG. 4 is a perspective view of a sample distributing valve according to a first embodiment of the present invention;

FIG. 5 is a front view of a sample distributing valve according to a first embodiment of the present invention;

FIG. 6 is a cross-sectional view taken along line A-A of FIG. 5;

FIG. 7 is a schematic diagram showing a structure of a sample transfer unit in a nucleic acid reagent reaction apparatus according to a second embodiment of the present invention.

List of parts and reference numerals:

1. a sample transfer unit; 1-1, accommodating a tube; 1-2, a piston; 1-3, a push-pull rod; 1-4, a sample separating valve; 1-4-1, valve core; 1-4-2, a first via; 1-4-3, a second through hole; 1-5, a sampling tube; 1-6, adding a sample tube; 1-7, a sample adding head; 1-8, sample adding holes; 1-9, a sample injection needle; 1-10, a second sealing ring; 1-11, a third sealing ring; 1-12, a first sealing ring; 1-13 and a second clamping pin; 1-14, a first bayonet; 1-15, a first one-way valve; 1-16, a first connection segment; 1-17, a second connecting segment; 1-18, a third connecting segment; 1-19, a fourth connecting segment; 1-20, a second one-way valve; 2. a reaction unit; 2-1, a reaction tube; 2-2, vacuum hatch; 2-3, pipe orifice; 2-4, first sealing paper; 2-5, second sealing paper; 2-6, a second card slot; 3. a lysis cup; 3-1, a cup mouth; 3-2, third sealing paper; 3-3 a first card slot.

Detailed Description

The present invention will be described in detail with reference to the following examples, but the present invention is not limited to these examples.

The utility model discloses a nucleic acid reagent reaction unit, include: the device comprises a sample transfer unit 1, a vacuum-sealed reaction unit 2 and a vacuum-sealed sample storage unit;

the sample transfer unit 1 comprises a containing tube 1-1, a liquid absorbing/discharging module, a sample feeding module and a sample adding module which are communicated with the containing tube 1-1, and the specific structure of the sample transfer unit is shown in figure 1; valves are arranged on the communicating pipelines of the sample introduction module, the sample adding module and the accommodating tube 1-1; the valve is used for controlling the accommodating tube 1-1 to be communicated with only one of the sample feeding module and the sample adding module at the same time. The valve may be a sample distributing valve 1-4, for example, a cylindrical sample distributing valve inserted into the communication line, and the structure thereof is shown in fig. 4 to 6. The shell of the sample distributing valve is hermetically connected with the sample inlet pipes 1-5 and the sample adding pipes 1-6, and is provided with a first liquid port opposite to the shell positioned in the sample inlet pipe and a second liquid port opposite to the shell positioned in the sample adding pipe. A valve core 1-4-1 of the sample separating valve 1-4 is cylindrical, and a first through hole 1-4-2 and a second through hole 1-4-3 are sequentially arranged along the axial direction of the valve core 1-4-1; the plane of the orifice of the first through hole 1-4-2 is vertical to the plane of the orifice of the second through hole 1-4-3; the first through hole 1-4-2 is used for communicating the sample introduction module with the accommodating tube 1-1; the second through hole 1-4-3 is used for communicating the sample adding module and the accommodating tube 1-1. After the first through hole 1-4-2 corresponds to the first liquid port on the shell, the sample injection module and the accommodating tube 1-1 can be communicated, and at the moment, the second through hole 1-4-3 does not correspond to the second liquid port on the shell, so that the sample injection module and the accommodating tube are not communicated. When the valve core 1-4-1 is rotated by 90 degrees, the second through hole 1-4-3 corresponds to the second liquid port on the shell, the sample adding module is communicated with the accommodating tube, at the moment, the first through hole 1-4-2 does not correspond to the first liquid port on the shell, and the sample feeding module is not communicated with the accommodating tube 1-1. That is, only one corresponding module connected with the through hole is communicated with the accommodating pipe 1-1 at the same time. More specifically, the rotation angles of the sample separating valves 1-4 can switch the flow conduction direction of the liquid path to control the sample introduction and the sample adding of the sample; specifically, a valve core of the sample distributing valve 1-4 is a cylinder, through holes which are 90 degrees at intervals are formed between the valve core and the circular surface, the sample distributing valve 1-4 is arranged at the inlet and outlet ends of the accommodating pipe 1-1, two axes are 90 degrees and intersect, the initial position of the sample distributing valve 1-4 is that one through hole is directly communicated with the sample inlet pipe, and the sample adding pipe is sealed by the circular surface of the sample distributing valve 1-4, so that a sample can be introduced; the sample distributing valve 1-4 rotates in one direction for 90 degrees for limiting, the other through hole of the sample distributing valve 1-4 is directly communicated with the sample adding pipe, and the sample feeding pipe is sealed by the circular surface of the sample distributing valve 1-4, so that a sample can be added. Of course, it may be a two-way check valve of other structure in the prior art; the sample introduction module extends into the sample storage unit and is connected with the sample storage unit in a sealing mode; the sample adding module extends into the reaction unit 2 and is hermetically connected with the reaction unit 2; and the liquid suction/discharge module is used for sucking the sample in the sample storage unit into the accommodating pipe 1-1 and discharging the sample in the accommodating pipe 1-1 to the reaction unit 2.

The sample introduction module comprises sample introduction needles 1-9, sample introduction pipes 1-5, a first sample introduction connecting piece and first sealing rings 1-12 sleeved outside the first sample introduction connecting piece; wherein, one end of the sample inlet pipe 1-5 is communicated with the accommodating pipe 1-1, and the other end is communicated with the sample inlet needle 1-9 for transmitting samples; the sample injection needle 1-9 is fixedly connected with the first sample injection connecting piece, is a slender needle with a pore inside and capable of puncturing sealing paper, is made of plastic or metal and is used for extending into the sample storage unit and transmitting a sample in the sample storage unit into the accommodating tube 1-1 through the sample injection tube 1-5; when the sample injection needle 1-9 extends into the sample storage unit, the first sealing ring 1-12 on the first sample injection connecting piece can seal the gap between the first sample injection connecting piece and the sample storage unit, so that the phenomenon that the external air continuously penetrates into the needle hole of the sample storage unit from the sample injection needle 1-9 and enters the sample storage unit to cause the pollution to the sample is avoided.

Further, the sample storage unit of the application is shown in the figure 3 in structure, and comprises a vacuum-sealed cracking cup 3 and a second sample introduction connecting piece which is arranged on the periphery of a cup opening 3-1 of the cracking cup for a circle and is connected with the cup opening 3-1 of the cracking cup; the inner diameter of the second sample introduction connecting piece is larger than that of the cracking cup 3; as the interior of the cracking cup 3 is a vacuum sealed environment, if a sample needs to be extracted from the interior of the cracking cup, the sample injection needle 1-9 needs to be extended into the cracking cup 3, therefore, the cup mouth 3-1 of the vacuum cracking cup is sealed by using third sealing paper 3-2, and at the moment, the sample injection needle 1-9 can pass through the third sealing paper 3-2 to extract the sample from the cracking cup 3. The lysis cup 3 contains lysis solution and a sample added later. The first sample introduction connecting piece comprises a first connecting section 1-16 and a second connecting section 1-17 which are connected with each other, and the first connecting section corresponds to the structure of the sample storage unit; the first sealing ring 1-12 is sleeved at the joint of the first connecting section 1-16 and the second connecting section 1-17; the outer diameters of the first connecting sections 1-16 are matched with the inner diameter of the second sample introduction connecting piece; the outer diameter of the second connecting sections 1-17 matches the inner diameter of the lysis cup 3. The connection part of the first connecting section 1-16 and the second connecting section 1-17 is in a step-shaped structure. The lower edge of the sealing ring can be rectangular, square, circular or oval, and the corresponding sealing ring can be correspondingly changed.

In order to ensure the connection stability of the sample storage unit and the sample injection unit and avoid the separation of the sample storage unit and the sample injection unit when oscillation occurs, so that the outside air pollutes the sample, the sample clamping device is also provided with a first clamping assembly; the first clamping assembly comprises first clamping pins 1-14 and first clamping grooves 3-3 matched with the first clamping pins 1-14; the first clamping pins 1-14 are arranged on the outer wall of the first connecting section 1-16; the first clamping groove 3-3 is arranged on the side wall of the second sample introduction connecting piece, and the arrangement position of the first clamping groove 3-3 corresponds to the arrangement position of the first clamping pin 1-14. The clamping connection of the first clamping pins 1-14 and the first clamping grooves 3-3 can ensure the connection stability of the sample storage unit and the sample injection unit.

Furthermore, the sample adding module comprises a sample adding head 1-7, a sample adding pipe 1-6, a first sample adding connecting piece and a second sealing ring 1-10 sleeved outside the first sample adding connecting piece; one end of the sample adding pipe 1-6 is communicated with the accommodating pipe 1-1, and the other end is communicated with the sample adding head 1-7; the sample adding head 1-7 is fixedly connected with the first sample adding connecting piece, the sample adding head is a sample adding head 1-7 with a sample adding needle inside, and is used for extending into the reaction unit 2 and transmitting the sample in the accommodating tube 1-1 to the reaction unit 2 through the sample adding tube 1-6; and the second sealing ring 1-10 is used for sealing the gap between the first sample adding connecting piece and the reaction unit 2.

Wherein the first sample adding connecting piece comprises a third connecting section 1-18 and a fourth connecting section 1-19 which are connected with each other; the outer diameter of the fourth connecting sections 1 to 19 is smaller than that of the third connecting sections 1 to 18 and matches the inner diameter of the reaction unit 2; the connection part of the third connecting section 1-18 and the fourth connecting section 1-19 is in a ladder shape, and the second sealing ring 1-10 is sleeved at the connection part of the third connecting section 1-18 and the fourth connecting section 1-19 and used for sealing a gap between the first sample adding connecting piece and the reaction unit 2.

The reaction unit 2 of the present application, the structure of which is shown in fig. 2, comprises a vacuum chamber, a vacuum-sealed reaction tube 2-1 and a second sample adding connector; wherein the pipe orifice 2-3 of the reaction tube is arranged in the vacuum cabin, the inner diameter of the reaction tube 2-1 is larger than the maximum outer diameter of the sample adding head 1-7, a reaction reagent is arranged in the reaction tube 2-1, the reaction tube 2-1 in the embodiment is a cone-shaped test tube which is embedded into the bottom of the cabin body, the pipe body is exposed out of the cabin bottom, the pipe orifice 2-3 and the vacuum hatch 2-2 are in the same direction; the second sample adding connecting piece is arranged on the periphery of the vacuum hatch 2-2 for a circle and is connected with the vacuum hatch 2-2; the inner diameter of the second sample adding connecting piece is matched with the outer diameters of the third connecting sections 1-18; the inner diameter of the vacuum chamber is matched with the outer diameter of the fourth connecting section 1-19; the sample adding head 1-7 sequentially extends into the second sample adding connecting piece, the vacuum cabin and the reaction tube 2-1, and the sample in the accommodating tube 1-1 is transmitted into the reaction tube 2-1 through the sample adding tube 1-6. Wherein the shape of the connection of the second sample application connection and the vacuum chamber matches the shape of the connection of the third connection segment 1-18 and the fourth connection segment 1-19. Because the sample application head 1-7 needs to pass through the vacuum chamber and the vacuum-sealed reaction tube to send the sample into the reaction tube 2-1, the nozzle 2-3 of the vacuum reaction tube 2-1 is sealed with the second sealing paper 2-5, and the vacuum port 2-2 corresponding to the nozzle 2-3 of the reaction tube 2-1 is sealed with the first sealing paper 2-4, so that the sample application head 1-7 can add the sample into the reaction tube through the sealing paper.

In order to further ensure the vacuum property of the sample adding head 1-7 when adding samples to the reaction tube, the sample adding module also comprises a third sealing ring 1-11; the third sealing ring 1-11 is sleeved outside the sample adding head 1-7 and is positioned at one end close to the first sample adding connecting piece, and is used for sealing the gap between the reaction tube 2-1 and the first sample adding connecting piece.

The utility model also provides a second clamping component for ensuring the connection stability of the reaction unit 2 and the sample adding unit and avoiding the separation of the reaction unit 2 and the sample adding unit when oscillation occurs, so that the external air pollutes the sample; the second clamping assembly comprises second clamping pins 1-13 and second clamping grooves 2-6 matched with the second clamping pins 1-13; the second clamping pins 1-13 are arranged on the outer wall of the third connecting section 1-18; the second clamping groove 2-6 is arranged on the side wall of the second sample adding connecting piece, and the arrangement position of the second clamping groove 2-6 corresponds to the arrangement position of the second clamping pin 1-13.

The utility model discloses a realize the miniaturation and move the liquid to the liquid measure is moved in accurate control, and the diameter of injecing the application of sample hole 1-8 of the application of sample needle in the injecing sample head 1-7 and the pinhole of injection needle 1-9 is between 0.3-0.7 mm.

The liquid suction/discharge module of the present application includes: a piston 1-2 and a push-pull rod 1-3 connected with the piston 1-2; the piston 1-2 is arranged in the accommodating tube 1-1; the push-pull rod 1-3 extends out of the accommodating pipe 1-1; of course, the pipetting/tapping module can also use prior art vacuum pumps. In the embodiment of the application, the accommodating tube 1-1 is a circular cavity, the piston 1-2 is a cylinder, the piston 1-2 is tightly matched and movably sealed with the accommodating tube 1-1, the push-pull rod 1-3 is moved to make the piston 1-2 linearly move so as to generate negative pressure and positive pressure in the accommodating tube 1-1, and simultaneously the volume of the solution in the accommodating tube 1-1 is changed, the push-pull rod 1-3 can be used for limiting and controlling sample injection or sample application in a grading manner, and can also be used for controlling sample injection or sample application in a subdivision scale manner; meanwhile, in order to conveniently know the amount of the sample, a scale mark or a quantitative limit point is arranged on the shell opposite to the push-pull rod 1-3.

In the application, the number of the sample adding heads 1-7 is 1 or more; the number of the reaction tubes 2-1 corresponds to the number of the sample adding heads 1-7; when the number of the nucleic acid amplification products is multiple, multiple types of nucleic acid amplification detection can be carried out simultaneously, and mutual influence is avoided, so that the detection efficiency is greatly improved.

The shape of the sample adding heads 1-7 in the embodiment of the application is conical, and when the conical shape penetrates through two layers of sealing paper and is inserted into the reaction tube, the resistance is small, and the needle heads in the conical shape are protected. The concrete structure of conical application of sample head 1-7 is that the appearance is conical, and the root is the cylinder, and the head is the tip of taking the application of sample needle, and the pinhole of its application of sample needle is exactly application of sample hole 1-8, and two-layer sealing paper can be punctured to conical application of sample head 1-7, and the material can be plastics or metal.

FIG. 7 is a sample transfer unit 1 using a check valve instead of the sample separation valve 1-4 in the present application, wherein the outflow direction of the first check valve 1-15 installed at the sample storage unit side is connected to the sample inlet tube 1-5, and the inflow direction is located at the needle point side of the sample injection needle 1-9, the sample injection needle 1-9 passes through the third sealing paper 3-2 on the lysis cup 3, and the push-pull rod 1-3 is pulled back to perform sample suction; the inflow direction of a second one-way valve 1-20 arranged at the side of the reaction unit is connected with the sample adding channel, the outflow direction is positioned at the side of a sample adding head 1-7, the sample adding head 1-7 passes through a first sealing paper 2-4 and a second sealing paper 2-5, a push rod 1-3 is pushed forwards to control sample adding, and a sample is added into the reaction tube.

The working process of the device is as follows: a sample injection needle 1-9 penetrates through third sealing paper 3-2 at the opening of the cracking cup 3 to enter the cracking cup 3, a first clamping pin 1-14 is clamped into a first clamping groove 3-3, so that the sample transfer unit 1 is sealed with the cracking cup 3, a push-pull rod 1-3 is moved to control sample suction, and a sample enters an accommodating tube 1-1; the sample adding head 1-7 penetrates through the first sealing paper 2-4 and then penetrates through the second sealing paper 2-5; the second clamping pins 1-13 are clamped into the second clamping grooves 2-6, so that the sample transfer unit 1 and the reaction unit 2 are sealed, the push-pull rod 1-3 is moved to control sample adding, and a sample is added into the reaction tube; and carrying out nucleic acid amplification reaction on the sample and the nucleic acid reagent in a double-sealed environment.

The utility model discloses a nucleic acid reagent reaction unit, guarantee that nucleic acid amplification and testing process are totally closed, can prevent effectively that the nucleic acid amplification product from leaking, avoid cross contamination, and can effectively avoid the pollution among nucleic acid amplification and the testing process, satisfy the requirement of nucleic acid testing under the non-laboratory environment, the difficult problem of on-the-spot nucleic acid short-term test under the non-laboratory environment has been solved, it is clinical to move forward the nucleic acid testing volume tailor clothing, to the basic unit, it need not the laboratory environment to be one, the check-out time is shorter, the detection means is more convenient, the rate of accuracy is higher, the better nucleic acid testing iteration technique of security.

Claims

1. A nucleic acid reagent reaction apparatus, comprising: the device comprises a sample transfer unit, a vacuum-sealed reaction unit and a vacuum-sealed sample storage unit;

the sample transfer unit comprises an accommodating tube, a liquid suction/discharge module, a sample introduction module and a sample adding module, wherein the liquid suction/discharge module, the sample introduction module and the sample adding module are communicated with the accommodating tube; valves are arranged on the communicating pipelines of the sample introduction module, the sample adding module and the accommodating pipe; the valve is used for controlling the accommodating tube to be communicated with the sample feeding module or one of the sample feeding modules at the same time;

2. The nucleic acid reagent reaction device according to claim 1, wherein the sample introduction module comprises a sample introduction needle, a sample introduction tube, a first sample introduction connector and a first sealing ring sleeved outside the first sample introduction connector;

the first sample introduction connecting piece is connected with the sample storage unit in a sealing mode through the first sealing ring.

3. The nucleic acid reagent reaction device according to claim 2, wherein the sample storage unit comprises a vacuum-sealed lysis cup and a second sample introduction connector which is arranged around the periphery of a cup opening of the lysis cup and is connected with the cup opening of the lysis cup; the inner diameter of the second sample introduction connecting piece is larger than that of the cracking cup;

4. The nucleic acid reagent reaction device according to claim 3, further comprising a first clamping assembly;

5. The nucleic acid reagent reaction device according to any one of claims 1 to 4, wherein the sample application module comprises a sample application head, a sample application tube, a first sample application connector, and a second sealing ring sleeved outside the first sample application connector;

6. The nucleic acid reagent reaction device according to claim 5, wherein the first sample addition connecting member comprises a third connecting segment and a fourth connecting segment connected to each other;

the second sealing ring is sleeved at the joint of the third connecting section and the fourth connecting section.

7. The nucleic acid reagent reaction device according to claim 6, wherein the reaction unit comprises a vacuum chamber, a vacuum-sealed reaction tube, and a second sample addition connection member;

8. The nucleic acid reagent reaction device according to claim 7, wherein the sample addition module further comprises a third sealing ring;

9. The nucleic acid reagent reaction device according to claim 8, further comprising a second clamping assembly;

10. The nucleic acid reagent reaction apparatus according to any one of claims 7 to 9, wherein the pipetting/pipetting module includes: the piston and a push-pull rod connected with the piston;

the piston is arranged in the accommodating pipe;

the push-pull rod extends out of the accommodating pipe.

11. The nucleic acid reagent reaction apparatus according to claim 10,

the number of the sample adding heads is 1 or more; the number of the reaction tubes corresponds to the number of the sample adding heads.

12. The nucleic acid reagent reaction apparatus according to claim 11,

the shape of the sample adding head is conical.

13. The nucleic acid reagent reaction apparatus according to claim 12,

the valve comprises a first one-way valve and a second one-way valve;

the second one-way valve is arranged on a communication pipeline of the sample adding module and the accommodating pipe and used for controlling the sample to be discharged from the accommodating pipe.

14. The nucleic acid reagent reaction apparatus according to claim 13,

the valve core of the valve is cylindrical, and a first through hole and a second through hole are sequentially arranged along the axial direction of the valve core;