CN217646431U - Handheld micro-fluidic chip nucleic acid detection device - Google Patents
Handheld micro-fluidic chip nucleic acid detection device Download PDFInfo
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- CN217646431U CN217646431U CN202221327489.XU CN202221327489U CN217646431U CN 217646431 U CN217646431 U CN 217646431U CN 202221327489 U CN202221327489 U CN 202221327489U CN 217646431 U CN217646431 U CN 217646431U
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Abstract
The application belongs to the technical field of micro-fluidic chips and relates to a handheld micro-fluidic chip nucleic acid detection device. The application provides a handheld micro-fluidic chip nucleic acid detection device includes: a sample collection tube; the chip connector is provided with a puncture tube for puncturing the sample collecting tube; the detection chip is connected with the chip connector and provided with a plurality of cavities and a plurality of runners, and the runners are communicated with the puncture tube and the cavities. The reaction reagent and all reaction processes are integrated on one chip to be completed, operations such as liquid injection and liquid transfer are not needed by means of external instruments, nucleic acid detection can be carried out without professional experimental places and special conditions, the handheld microfluidic chip nucleic acid detection device can be used in community hospitals, family self-inspection, public places and the like for popularization and application, real-time screening and detection during epidemic situation prevention and control and personal health condition self-inspection are realized.
Description
Technical Field
The application relates to the technical field of microfluidic chips, in particular to a handheld microfluidic chip nucleic acid detection device.
Background
Microfluidic chips, also known as Lab-on-a-chips, refer to a biological or chemical laboratory built on a few square centimeters Chip. It integrates the basic operation units of reaction, separation, culture, sorting and detection, etc. related in the biological and chemical fields into a very small chip, and the microchannels form a network to control the fluid to penetrate through the whole system, so as to implement various functions of routine biological or chemical laboratories. The micro-fluidic chip technology has the characteristics of small sample volume, high integration level, easy realization of automatic control, high-flux analysis and the like, so that the biochemical reaction operation on the micro-fluidic chip is more convenient and rapid than the conventional analysis sample pretreatment, and the cost is low. The microfluidic technology can realize the complex operation steps of reagent reaction, separation, detection and the like on one chip, so that the sample processing time is greatly shortened, the cost of reagents and instruments is greatly reduced, and the detection resolution and sensitivity are obviously improved. The volume of the detection device is greatly reduced, portability and field detection become possible, and automation and portability of nucleic acid detection can be realized.
At present, molecular diagnostic techniques based on nucleic acid detection are widely used in life, including clinical detection and analysis, food safety, environmental detection, drug screening, and other fields. The nucleic acid detection process generally comprises steps of pathogen sampling, sample nucleic acid extraction, PCR amplification, post signal analysis, result analysis and the like. Traditional nucleic acid detection needs to be completed in a partition PCR laboratory provided with a specialty, expensive equipment and instruments such as a PCR instrument need to be configured in the laboratory, and a series of operation processes are complex, the operation is complex, and detection needs to be completed by a professional. The harsh detection conditions lead to that only part of hospitals have qualification and capability to carry out nucleic acid detection at present, the detection is very complicated once and needs a special person to carry out the detection, and in the face of severe new crown epidemic situations, a nucleic acid self-detection device which is convenient for users to operate is urgently needed.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a handheld micro-fluidic chip nucleic acid detection device, it is loaded down with trivial details to solve current nucleic acid testing process, and the operation is complicated, needs the technical problem that the professional accomplished the detection.
In order to solve the above-mentioned problem, the embodiment of the present invention provides the following technical solutions:
a handheld microfluidic chip nucleic acid detection device, comprising:
a sample collection tube;
the chip connector is provided with a puncture tube for puncturing the sample collecting tube;
the detection chip is connected with the chip connector and provided with a plurality of cavities and a plurality of runners, and the runners are communicated with the puncture tube and the cavities.
Further, the sample collection pipe comprises a pipe body, a collection liquid pipe and a pipe cover, the collection liquid pipe is arranged in the pipe body, the pipe cover is rotatably arranged on the pipe body, and the pipe cover is used for covering the collection liquid pipe.
Further, handheld micro-fluidic chip nucleic acid detection device still includes collection tube sealing film and collection tube sealing film, collection tube sealing film and collection tube sealing film are located respectively in the tube cap with collection liquid pipe bottom, collection tube sealing film and collection tube sealing film are used for right respectively collection liquid pipe's oral area and bottom seal.
Furthermore, the detection chip comprises a first chip and a second chip, the first chip is connected with the second chip, and the cavity is arranged in the first chip.
Further, the sample collection tube further comprises a gas buffer tube, and the gas buffer tube is arranged in the tube body;
the runner includes access way, fluid outlet channel, first interface channel and second interface channel, access way with puncture tube intercommunication, fluid outlet channel with gaseous buffer tube intercommunication, first interface channel is used for connecting access channel and cavity, first interface channel is used for connecting fluid outlet channel and cavity.
Further, the chambers are connected in series or in parallel with the channels; the chamber is pre-filled with fixed biochemical reaction reagents, wherein the reagents are in a powdery, liquid, freeze-dried ball or solid particle state;
the collecting liquid pipe, the puncture pipe and the sample feeding channel are arranged along the vertical direction, and a reagent in the collecting liquid pipe flows into the reaction and detection chamber through the sample feeding channel on the detection chip under the action of gravity through the puncture pipe;
the width of the flow channel is more than 0 and less than or equal to 10mm, and the depth of the chip micro-flow channel is more than 0 and less than or equal to 10mm.
Furthermore, the detection chip also comprises a double-sided adhesive film, and the first chip and the second chip are connected through the double-sided adhesive film;
or the first chip and the second chip are connected in a laser welding mode, an ultrasonic welding mode, a hot-pressing bonding mode, a plasma bonding mode and a solvent bonding mode.
Further, the handheld micro-fluidic chip nucleic acid detection device further comprises a sealing silica gel gasket, the sealing silica gel gasket is arranged between the sample collection tube and the chip connector, the sealing silica gel gasket is provided with a first through hole and a second through hole, the first through hole is matched with the puncture tube, the chip connector is provided with a gas outlet communicated with the fluid outlet channel, and the second through hole is matched with the gas outlet.
Further, a hydrophobic air-permeable membrane is arranged at the air outlet, and the hydrophobic air-permeable membrane seals one end of the gas buffer tube facing the chip connector;
the thickness of the hydrophobic breathable film is more than 0 and less than or equal to 1mm.
Further, a sealing ring is arranged between the chip connector and the detection chip.
Furthermore, the hydrophobic breathable film is made of polytetrafluoroethylene, silicon rubber or polyethylene;
the sealing silica gel gasket and the sealing ring are made of chloroprene rubber, natural rubber, EPDM rubber or acrylate rubber.
Furthermore, the outer side of the chip connector and the inner side of the sample collection tube are provided with buckling structures.
Further, the chip connector orientation the one end of detecting the chip is equipped with the confession detect the mounting groove of chip installation, detect the chip orientation chip connector one end is equipped with first draw-in groove and second draw-in groove, the chip connector orientation the one end of detecting the chip be equipped with first draw-in groove complex first fixture block and with second draw-in groove complex second fixture block, the sealing washer be equipped with in the first draw-in groove and with the laminating of first fixture block, the second fixture block is equipped with the air vent, the air vent both ends respectively with fluid outlet channel and gas outlet intercommunication.
Compared with the prior art, the embodiment of the utility model provides a mainly have following beneficial effect:
a hand-held micro-fluidic chip nucleic acid detection device integrates reaction reagents and all reaction processes on one chip, operation such as liquid injection and liquid transfer is not needed by means of an external instrument, nucleic acid detection can be carried out without professional experimental places and special conditions, specifically, a sampling solution containing nucleic acid to be detected is added into a sample collection tube, a swab head of a test swab is added into the collection tube, the sample collection tube is downwards inserted by aiming at a chip connector, under the action of a puncture tube, reaction liquid in the sample collection tube flows into a chamber for reaction and detection through a flow channel under the action of gravity, reacts with the pre-loaded reagent in the chamber, and then the chip is inserted into an instrument matched with the chip, so that the process of nucleic acid detection is realized; the utility model discloses simplify nucleic acid detection's operating procedure greatly, do not have too big requirement to operating personnel and place, operating personnel need not professional training and can the operation of getting on the hand, have characteristics such as easy operation, detection efficiency height, sensitivity height, handheld micro-fluidic chip nucleic acid detection device can be used in community's hospital, family's self-checking, public place etc. and popularize and apply, realizes real-time examination and detection during epidemic situation prevention and control to and individual health situation self-test.
Drawings
In order to illustrate the solution of the present invention more clearly, the drawings needed for describing the embodiments will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
Fig. 1 is a schematic diagram of the overall structure of a handheld microfluidic chip nucleic acid detection device according to an embodiment of the present invention;
FIG. 2 is an exploded view of a handheld microfluidic chip nucleic acid detecting device according to an embodiment of the present invention;
FIG. 3 is a schematic diagram of the internal structure of the handheld microfluidic chip nucleic acid detection device according to an embodiment of the present invention;
fig. 4 is a schematic structural view of a sample collection tube according to an embodiment of the present invention;
fig. 5 is a schematic structural diagram of a detection chip according to an embodiment of the present invention.
Description of reference numerals:
1. a sample collection tube; 11. a pipe body; 12. collecting a liquid pipe; 13. a tube cover; 14. a gas buffer tube; 2. a chip connector; 21. a puncture tube; 22. an air outlet; 23. mounting grooves; 24. a first clamping block; 25. a second fixture block; 3. detecting the chip; 31. a first chip; 32. a second chip; 33. a chamber; 34. a flow channel; 341. a sample introduction channel; 342. a fluid outlet passage; 343. a first connecting channel; 344. a second connecting channel; 35. double-sided adhesive films; 36. a first card slot; 37. a second card slot; 4. collecting tube sealing film; 5. sealing a membrane by the collecting tube; 6. sealing a silica gel gasket; 61. a first through hole; 62. a second through hole; 7. a hydrophobic, breathable film; 8. and (5) sealing rings.
Detailed Description
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The terms "including" and "having," and any variations thereof in the description and claims of the invention and the description of the figures appended hereto, are intended to cover non-exclusive inclusions. The terms "first," "second," and the like in the description and in the claims, or in the drawings, are used for distinguishing between different objects and not necessarily for describing a particular sequential or chronological order.
Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the invention. The appearances of the phrase in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. It is explicitly and implicitly understood by one skilled in the art that the embodiments described herein can be combined with other embodiments.
In order to make the technical solution of the present invention better understood by those skilled in the art, the technical solution of the embodiments of the present invention will be clearly and completely described below with reference to the related drawings.
Examples
As shown in fig. 1 to 3, a handheld microfluidic chip nucleic acid detection device comprises a sample collection tube 1, a chip connector 2 and a detection chip 3; the chip connector 2 is provided with a puncture tube 21 for puncturing the sample collecting tube 1; the detection chip 3 is connected with the chip connector 2, the detection chip 3 is provided with a plurality of cavities 33 and a plurality of runners 34, and the runners 34 are communicated with the puncture tube 21 and the cavities 33.
The embodiment of the utility model provides a handheld micro-fluidic chip nucleic acid detection device, concentrate reactant and all reaction processes and accomplish on a chip, need not to annotate liquid with the help of external instrument, shift operations such as liquid, need not professional laboratory space and special conditions and just can carry out nucleic acid testing, it is concrete, add the sampling solution that contains the nucleic acid that awaits measuring in sample collection pipe 1, add the cotton swab head of test in gathering liquid pipe 12, aim at chip connector 2 with sample collection pipe 1 and insert downwards, under puncture pipe 21 effect, reaction liquid in sample collection pipe 1 flows in the cavity 33 that is used for reaction and detection through runner 34 under the action of gravity, take place nucleic acid reaction with the pre-installed reagent in cavity 33, then insert the chip in rather than supporting instrument, realize the process of nucleic acid testing; the utility model discloses simplify nucleic acid detection's operating procedure greatly, do not have too big requirement to operating personnel and place, operating personnel need not professional training and can the operation of getting on the hand, have characteristics such as easy operation, detection efficiency height, sensitivity height, handheld micro-fluidic chip nucleic acid detection device can be used in community's hospital, family's self-checking, public place etc. and popularize and apply, realizes real-time examination and detection during epidemic situation prevention and control to and individual health situation self-test.
As shown in fig. 1 and 4, the sample collection tube 1 includes a tube body 11, a collection liquid tube 12, a tube cover 13 and a gas buffer tube 14, the collection liquid tube 12 is disposed in the tube body 11, the tube cover 13 is rotatably disposed on the tube body 11, the tube cover 13 is used for covering the collection liquid tube 12, and the gas buffer tube 14 is disposed in the tube body 11;
in one embodiment, the collection tube 12 contains a lysis solution, a sampling solution containing nucleic acid to be detected can be added into the collection tube 12, the tube cap 13 is opened, the swab head is immersed into the sampling solution, rotated and uniformly mixed for several seconds and then broken off in the collection tube 12, and then the collection tube cap 13 is closed, so that sample lysis is realized.
In one embodiment, a collection tube 12 and a gas buffer tube 14 are disposed side-by-side within the tube body 11.
As shown in fig. 2 and 3, the handheld microfluidic chip nucleic acid detecting device further includes a collecting tube sealing film 4 and a collecting tube sealing film 5, where the collecting tube sealing film 4 and the collecting tube sealing film 5 are respectively used for sealing the opening and the bottom of the collecting liquid tube 12.
In one embodiment, the collection tube closure membrane 4 is pre-installed within the cap 13, and a seal is formed by the collection tube closure membrane 4 to prevent contact with the outside air and leakage.
In one embodiment, the sample collection tube 1 is preloaded with a collection tube sealing membrane 5 at the bottom, the collection tube sealing membrane 5 preventing collection liquid from leaking out of the collection liquid tube 12.
And a sealing ring 8 is arranged between the chip connector 2 and the detection chip 3. And under the action of the sealing ring 8, the lower surface of the inner side of the chip connector 2 is completely sealed with the detection chip 3.
In one embodiment, the sealing ring 8 is an O-ring 8, and the material of the sealing ring 8 is neoprene, natural rubber, EPDM rubber or acrylate rubber.
As shown in fig. 1 and fig. 2, the detection chip 3 includes a first chip 31 and a second chip 32, the first chip 31 is connected to the second chip 32, and the cavity 33 is disposed in the first chip 31.
In one embodiment, the material of the first chip 31 is glass, silicon wafer, polymer, metal or metal oxide.
In one embodiment, the material of the second chip 32 is glass, silicon wafer, polymer, metal or metal oxide.
With reference to fig. 3 and 5, the flow channel 34 includes a sample channel 341, a fluid outlet channel 342, a first connection channel 343 and a second connection channel 344, the sample channel 341 is communicated with the puncture tube 21, the fluid outlet channel 342 is communicated with the gas buffer tube 14, the first connection channel 343 is used for connecting the sample channel 341 and the chamber 33, and the first connection channel 343 is used for connecting the fluid outlet channel 342 and the chamber 33.
In one embodiment, the chamber 33 is connected in series or in parallel with the channel; the chamber 33 is filled with a fixed biochemical reaction reagent.
In one embodiment, the runner 34 may be machined by machine tool machining, laser ablation, 3D printing, injection molding, or chemical etching.
In one embodiment, the flow channels 34 are micro channels, the width of the flow channels 34 is greater than 0 and less than or equal to 10mm, and the depth of the chip micro channels 34 is greater than 0 and less than or equal to 10mm.
In one embodiment, the collection liquid tube 12, the puncture tube 21 and the sample channel 341 are arranged in a vertical direction, and the reagent in the collection liquid tube 12 flows into the reaction and detection chamber 33 through the sample channel 341 on the detection chip 3 under the action of gravity through the puncture tube 21.
The handheld micro-fluidic chip nucleic acid detection device further comprises a sealing silica gel gasket 6, the sealing silica gel gasket 6 is arranged between the sample collection tube 1 and the chip connector 2, the sealing silica gel gasket 6 is provided with a first through hole 61 and a second through hole 62, the first through hole 61 is matched with the puncture tube 21, the chip connector 2 is provided with a gas outlet 22 communicated with the fluid outlet channel 342, and the second through hole 62 is matched with the gas outlet 22.
In one embodiment, the first and second through holes 61 and 62 may be round or square holes, and in particular, may be configured to have a shape that fits the puncture tube 21 and the air outlet 22.
In one embodiment, the silicone gasket 6 is made of neoprene, natural rubber, EPDM rubber or acrylate rubber.
In one embodiment, the fluid outlet channel 342 exits through the chip connector 2 in correspondence with a through hole in the lower end of the gas buffer tube 14, forming a gas flow area.
The gas outlet 22 of the chip connector 2 is provided with a hydrophobic breathable film 7, and the hydrophobic breathable film 7 seals the gas buffer tube 14 towards one end of the chip connector 2 to prevent liquid from passing through.
One open end of the flow channel 34 is a sample inlet, and the other open end extends to the outside of the detection chip 3 and is connected with the hydrophobic gas-permeable membrane 7, i.e. one open end is disposed in the sample inlet channel 341, and the other open end is disposed in the fluid outlet channel 342.
In one embodiment, the hydrophobic and air permeable membrane 7 is made of teflon, silicone rubber or polyethylene.
Preferably, the thickness of the hydrophobic breathable film 7 is greater than 0 and less than or equal to 1mm, and the hydrophobic breathable film 7 can allow air to pass through but can prevent liquid from passing through.
In one embodiment, the chamber 33 is filled with a biochemical reagent, which may be in various forms such as powder, liquid, lyophilized pellet, solid particle, etc., and the liquid in the collecting liquid tube 12 flows into the chamber and then reacts with the chamber.
In one embodiment, the detection chip 3 further includes a double-sided adhesive film 35, and the first chip 31 and the second chip 32 are connected by the double-sided adhesive film 35.
In one embodiment, the first chip 31 and the second chip 32 are connected by laser welding, ultrasonic welding, thermocompression bonding, plasma bonding, solvent bonding, or the like.
The outside of chip connector 2 and the inboard of sample acquisition pipe 1 are equipped with buckle structure, and when sample acquisition pipe 1 down inserted chip connector 2, both passed through buckle structure and locked.
It should be noted that, the handheld microfluidic chip nucleic acid detecting device can be in the shape of a rectangular box, but the handheld microfluidic chip nucleic acid detecting device can also be adjusted for specific applications without departing from the essence of the present invention, for example, the following adjustments can be made: the shape of the handheld microfluidic chip nucleic acid detection device can be adjusted according to the processing mode and functional requirements, and can be combined into a cuboid, a cylinder and other shapes; the shape of the flow channel 34 on the detection chip 3 can be adjusted correspondingly according to the functional requirements; the number of the chambers 33 on the detection chip 3 can be adjusted according to actual needs.
The embodiment of the utility model provides a handheld micro-fluidic chip nucleic acid detection device has realized application of sample and testing process's accurate control, easy operation, and sensitivity is high, need not professional training and can go up the manual operation, and detection efficiency is high, and sample consumption is few, can carry out nucleic acid self-checking fast, and application scope is wide. The handheld microfluidic chip nucleic acid detection device can be applied to various hospitals, import and export customs and disease control centers, and can also be used for various field detection scenes such as home self-inspection, community detection, outdoor monitoring and the like.
The embodiment of the utility model provides a handheld micro-fluidic chip nucleic acid detection device, application method as follows:
adding a sampling solution containing nucleic acid to be detected into the sample collection tube, and sampling the sample;
installing the chip connector and the detection chip, and aligning the sample collection tube with the chip connector to ensure that the puncture tube punctures the sample collection tube;
the reaction liquid in the sample collection tube flows into the cavity through the flow channel on the detection chip under the action of gravity, and the reaction liquid and the pre-loaded reagent in the cavity are subjected to nucleic acid reaction;
and inserting the detection chip into an instrument matched with the detection chip to obtain a detection result.
The step of adding a sampling solution containing nucleic acid to be detected into the sample collection tube comprises the following steps: adding a sampling solution containing nucleic acid to be detected into the sample collecting tube, opening the tube cover, immersing the swab head into the sampling solution, rotating and uniformly mixing for several seconds, then breaking off the swab head in the sample collecting tube, and covering the tube cover.
In the step of mounting the chip connector and the detection chip, aligning the sample collection tube with the chip connector and enabling the puncture tube to puncture the sample collection tube, inserting the chip connector into the detection chip to realize the fixation and clamping of the chip connector and the detection chip, and sealing the chip connector and the detection chip through a sealing ring; the sample collection tube is inserted downwards by aligning with the chip connector, the sample collection tube and the chip connector are sealed by a sealing silica gel gasket, and the bottom sealing film of the collection tube is punctured under the action of the puncture tube.
The step of inserting the detection chip into a matched instrument to obtain the nucleic acid detection result comprises the following steps:
inserting the detection chip into an instrument matched with the detection chip, and heating the cavity to a set temperature by the instrument to perform nucleic acid amplification reaction;
an optical detection module on the instrument detects that the absorbance change of the reaction solution generates a color development reaction, and a detection result is obtained according to the color.
It is to be understood that the above-described embodiments are only some of the embodiments of the present invention, and not all of the embodiments, and the preferred embodiments of the present invention are shown in the drawings, but not limited to the scope of the present invention. The present invention may be embodied in many different forms and, on the contrary, these embodiments are provided so that this disclosure will be thorough and complete. Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes in the embodiments and modifications can be made, and equivalents may be substituted for elements thereof. All utilize the equivalent structure that the content of the utility model discloses a specification and attached drawing was done, direct or indirect application is in other relevant technical field, all is in the same way the utility model discloses within the patent protection scope.
Claims (13)
1. A handheld microfluidic chip nucleic acid detection device, comprising:
a sample collection tube;
the chip connector is provided with a puncture tube for puncturing the sample collecting tube;
the detection chip is connected with the chip connector and provided with a plurality of cavities and a plurality of runners, and the runners are communicated with the puncture tube and the cavities.
2. The handheld microfluidic chip nucleic acid detection device of claim 1,
the sample collection pipe comprises a pipe body, a collection liquid pipe and a pipe cover, the collection liquid pipe is arranged in the pipe body, the pipe cover is rotatably arranged on the pipe body, and the pipe cover is used for covering the collection liquid pipe.
3. The handheld microfluidic chip nucleic acid detecting device according to claim 2,
the handheld micro-fluidic chip nucleic acid detection device further comprises a collection tube sealing film and a collection tube sealing film, wherein the collection tube sealing film and the collection tube sealing film are respectively arranged in the tube cover and at the bottom of the collection liquid tube, and the collection tube sealing film are respectively used for sealing the opening and the bottom of the collection liquid tube.
4. The handheld microfluidic chip nucleic acid detecting device according to claim 1,
the detection chip comprises a first chip and a second chip, the first chip is connected with the second chip, and the cavity is arranged in the first chip.
5. The handheld microfluidic chip nucleic acid detection device of claim 2,
the sample collection tube further comprises a gas buffer tube, and the gas buffer tube is arranged in the tube body;
the runner includes access way, fluid outlet channel, first interface channel and second interface channel, access way with puncture tube intercommunication, fluid outlet channel with gaseous buffer tube intercommunication, first interface channel is used for connecting access channel and cavity, first interface channel is used for connecting fluid outlet channel and cavity.
6. The handheld microfluidic chip nucleic acid detecting device according to claim 5,
the chambers are connected in series or in parallel with the channels; the chamber is pre-filled with fixed biochemical reaction reagents, wherein the reagents are in a powdery, liquid, freeze-dried ball or solid particle state;
the collecting liquid pipe, the puncture pipe and the sample feeding channel are arranged along the vertical direction, and a reagent in the collecting liquid pipe flows into the reaction and detection chamber through the sample feeding channel on the detection chip under the action of gravity through the puncture pipe;
the width of the flow channel is more than 0 and less than or equal to 10mm, and the depth of the chip micro-flow channel is more than 0 and less than or equal to 10mm.
7. The handheld microfluidic chip nucleic acid detecting device according to claim 4,
the detection chip further comprises a double-sided adhesive film, and the first chip and the second chip are connected through the double-sided adhesive film;
or the first chip and the second chip are connected in a laser welding mode, an ultrasonic welding mode, a hot-pressing bonding mode, a plasma bonding mode and a solvent bonding mode.
8. The handheld microfluidic chip nucleic acid detecting device according to claim 5,
the handheld micro-fluidic chip nucleic acid detection device further comprises a sealing silica gel gasket, the sealing silica gel gasket is arranged between the sample collection tube and the chip connector, the sealing silica gel gasket is provided with a first through hole and a second through hole, the first through hole is matched with the puncture tube, the chip connector is provided with a gas outlet communicated with the fluid outlet channel, and the second through hole is matched with the gas outlet.
9. The handheld microfluidic chip nucleic acid detection device of claim 8,
the gas outlet is provided with a hydrophobic gas-permeable membrane which seals one end of the gas buffer tube facing the chip connector;
the thickness of the hydrophobic breathable film is more than 0 and less than or equal to 1mm.
10. The handheld microfluidic chip nucleic acid detecting device according to claim 9,
and a sealing ring is arranged between the chip connector and the detection chip.
11. The handheld microfluidic chip nucleic acid detecting device of claim 10,
the hydrophobic breathable film is made of polytetrafluoroethylene, silicon rubber or polyethylene;
the sealing silica gel gasket and the sealing ring are made of chloroprene rubber, natural rubber, EPDM rubber or acrylate rubber.
12. The handheld microfluidic chip nucleic acid detection device of claim 1,
the outer side of the chip connector and the inner side of the sample collecting tube are provided with buckle structures.
13. The handheld microfluidic chip nucleic acid detecting device of claim 10,
the chip connector orientation the one end of detecting the chip is equipped with the confession detect the mounting groove of chip installation, detect the chip orientation chip connector one end is equipped with first draw-in groove and second draw-in groove, the chip connector orientation the one end of detecting the chip be equipped with first draw-in groove complex first fixture block and with second draw-in groove complex second fixture block, the sealing washer is equipped with in the first draw-in groove and with the laminating of first fixture block, the second fixture block is equipped with the air vent, the air vent both ends respectively with fluid outlet passageway and gas outlet intercommunication.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221327489.XU CN217646431U (en) | 2022-05-30 | 2022-05-30 | Handheld micro-fluidic chip nucleic acid detection device |
| PCT/CN2022/106129 WO2023231158A1 (en) | 2022-05-30 | 2022-07-15 | Handheld nucleic acid testing device having micro-fluidic chip, and use method therefor |
| US18/061,481 US20230383222A1 (en) | 2022-05-30 | 2022-12-05 | Hand-held nucleic acid detection apparatus equipped with microfluidic chip, and use method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221327489.XU CN217646431U (en) | 2022-05-30 | 2022-05-30 | Handheld micro-fluidic chip nucleic acid detection device |
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| Publication Number | Publication Date |
|---|---|
| CN217646431U true CN217646431U (en) | 2022-10-25 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221327489.XU Active CN217646431U (en) | 2022-05-30 | 2022-05-30 | Handheld micro-fluidic chip nucleic acid detection device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116396841A (en) * | 2023-06-07 | 2023-07-07 | 深圳市合川医疗科技有限公司 | Anaerobe sampling detection device |
| TWI822540B (en) * | 2022-12-30 | 2023-11-11 | 財團法人工業技術研究院 | Biopsy sample preprocessing device |
-
2022
- 2022-05-30 CN CN202221327489.XU patent/CN217646431U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI822540B (en) * | 2022-12-30 | 2023-11-11 | 財團法人工業技術研究院 | Biopsy sample preprocessing device |
| CN116396841A (en) * | 2023-06-07 | 2023-07-07 | 深圳市合川医疗科技有限公司 | Anaerobe sampling detection device |
| CN116396841B (en) * | 2023-06-07 | 2023-12-01 | 深圳市合川医疗科技有限公司 | Anaerobe sampling detection device |
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