CN215506829U

CN215506829U - Rapid detection reaction reagent tube

Info

Publication number: CN215506829U
Application number: CN202121851472.XU
Authority: CN
Inventors: 高秀洁
Original assignee: Guangdong Pharmaceutical University
Current assignee: Guangdong Pharmaceutical University
Priority date: 2021-08-09
Filing date: 2021-08-09
Publication date: 2022-01-14
Anticipated expiration: 2031-08-09

Abstract

The utility model provides a reagent tube for rapid detection reaction, which comprises a first reagent tube and a second reagent tube, wherein the first reagent tube comprises a test tube body, a puncture part and a sealing part, a containing cavity for placing a first reaction reagent is arranged in the test tube body, one end of the puncture part is fixedly connected with the test tube body, a through hole is arranged in the puncture part, the through hole is communicated with the containing cavity, the sealing part is detachably connected with one end of the puncture part, which is far away from the test tube body, and when the sealing part is arranged on the puncture part, the through hole can be sealed; the second reagent pipe comprises a pipe body, a reaction cavity with an opening at one end is arranged in the pipe body, the reaction cavity is used for placing a second reaction reagent, a sealing film is arranged at the opening of the pipe body, and the sealing film can be punctured by the puncturing part. The reaction reagent tube can realize rapid detection reaction, can effectively avoid the problem of sample or product pollution in the operation or reaction process, and improves the detection efficiency and accuracy.

Description

Rapid detection reaction reagent tube

Technical Field

The utility model relates to the technical field of experimental equipment, in particular to a rapid detection reaction reagent tube.

Background

At present, detection methods for food pollutants, pathogens, microorganisms, animal and plant quarantine and the like mainly comprise a conventional Real-Time fluorescence RT-PCR (Real-Time PCR), an ultra-fast Real-Time fluorescence RT-PCR method, a constant-temperature amplification detection method, a micro-fluidic chip and the like. These detection techniques involve the extraction and pretreatment of nucleic acid samples, mainly using the following three methods: silica gel membrane column method, magnetic bead adsorption method, and chemical cracking release method. The chemical lysis releasing method is relatively fast, and can complete the membrane breaking or lysis of cell, pathogen and microbe in sample in short time to release nucleic acid into solvent.

The conventional real-time fluorescence RT-PCR method consumes long time, each sample needs 2-3h, and the clinical report time for batch detection is generally 1-2 days; the ultra-fast real-time fluorescence RT-PCR method needs to be additionally provided with sample pretreatment and nucleic acid extraction steps and equipment. The automatic treatment equipment integrating the nucleic acid extraction and purification equipment, the amplification equipment and the liquid transfer device into a whole can meet the requirement of automatic operation, but still needs large-scale equipment which can only be realized in a specific test laboratory.

In order to accelerate the detection rate, technicians integrate a pretreatment reagent into a card box, and control a liquid circulation channel in the card box by means of instruments and equipment to realize the steps of extraction, amplification and detection, for example, a commercial Mycobacterium seepex nucleic acid detection product is convenient to use, special equipment matched with the kit is needed, the price of the reagent card box reaches more than 500 yuan/person, and the detection report time exceeds 1 hour. Also, technicians place the isothermal amplification product on a chromatographic test strip, and realize visual colloidal gold test strip chromatographic detection by detecting a specific marker signal on the product, but the product cannot be directly detected, and the lateral flow chromatography detection can be performed only after manual dilution or additional liquid dilution is needed, so that the product is likely to be polluted in the process.

The constant temperature technology amplification can directly observe the detection result through fluorescence, turbidimetry, color change and other modes, does not need special signal detection equipment to realize result interpretation, and is a more convenient detection method. The product or the device from sample pretreatment to detection result judgment can be conveniently realized in a short time, and the urgent requirements in the scenes of clinical rapid diagnosis, outpatient immediate detection, home self-test, rapid screening and the like can be better met. In the field of point of Clinical test, the rapid immunoassay products such as colloidal gold occupy an important position due to the advantages of convenient sampling, convenient operation, rapid detection, visual result and the like. The nucleic acid detection method has the advantages of high sensitivity and better specificity compared with an immunodetection method, but nucleic acid residue pollution, product pollution, sample cross-contamination and the like need to be avoided, a specific experimental site is needed for carrying out nucleic acid extraction and purification operations, and operators can carry out test work only through related training such as PCR (polymerase chain reaction), so that great limitation is brought to the application of molecular POCT. At present, related technical personnel integrate nucleic acid extraction and purification equipment, pipetting equipment and amplification equipment together to form an integrated equipment from sample treatment to result report, and matched reagents. But still requires specialized equipment to run the test, which is inconvenient for home self-testing or out-patient testing.

SUMMERY OF THE UTILITY MODEL

Therefore, there is a need to provide a rapid detection reaction reagent tube, which can achieve rapid detection reaction, effectively avoid the problem of sample or product contamination during operation or reaction, and improve detection efficiency and accuracy.

A rapid detection reaction reagent tube comprises

The first reagent tube comprises a test tube body, a puncture part and a sealing part, wherein an accommodating cavity for accommodating a first reaction reagent is arranged in the test tube body, one end of the puncture part is fixedly connected with the test tube body, a through hole is arranged in the puncture part, one end of the through hole is communicated with the accommodating cavity, the other end of the through hole is communicated with the outside, the sealing part is detachably connected with one end of the puncture part, which is far away from the test tube body, and the through hole can be sealed when the sealing part is arranged on the puncture part;

the second reagent tube comprises a tube body, wherein a reaction cavity with an opening at one end is arranged in the tube body, the reaction cavity is used for placing a second reaction reagent, a sealing film is arranged at the opening of the tube body, and the sealing film can be punctured by the puncturing part.

Above-mentioned reaction reagent pipe through setting up first reagent pipe and second reagent pipe, separately seals two kinds of reaction reagent and places, treats that to examine time measuring, pulls down the sealing portion of first reagent pipe, punctures the sealing membrane with puncture portion, makes this internal reaction reagent of test tube flow into the body again, contacts and reacts with second reaction reagent. The process is simple and easy to operate, the reaction reagent can be filled in advance according to the dosage, the operator can test without special training, and the operation success rate is high. Moreover, because the two reaction reagents are mixed only when in use, the two reaction reagents are put in place in one step, raw material or reaction pollution possibly generated in the operation process is greatly reduced, and the detection efficiency and accuracy are improved. In addition, the reaction equipment is miniaturized and operates quickly, and is favorable for realizing home self-testing, on-site screening and outpatient detection.

In one embodiment, the test tube body and the puncture portion are of an integrated structure. The structure is suitable for testing without adding samples or other reaction liquid, and further reduces the pollution possibly generated in the operation process.

In one embodiment, the puncturing part is screwed with the test tube body. The puncture part is detachably connected with the test tube body, so that a reagent can be conveniently filled in the test tube body, or a sample or other reaction reagents can be added before testing.

In one embodiment, a first external thread is arranged at one end of the test tube body close to the puncture part, and a first internal thread matched with the first external thread is arranged on the puncture part.

In one embodiment, the sealing portion is threadably connected to the piercing portion. The threaded connection is convenient to install and disassemble.

In one embodiment, a protruding portion is arranged at one end, far away from the test tube body, of the puncturing portion, a second external thread is arranged on the protruding portion, and a second internal thread matched with the second external thread is arranged on the sealing portion. The raised structure is more favorable for quickly puncturing the sealing film.

In one embodiment, the tube body is provided with a third internal thread matched with the second external thread, and the tube body can be in threaded connection with the puncture part. After the two reaction reagents are mixed, the puncture part can be screwed on the tube body, so that the reaction is carried out in a sealed environment, and the pollution is prevented.

In one embodiment, the height of the protruding part is 0.2 to 0.5 times the height of the reaction chamber. This arrangement is provided to prevent the reaction liquid in the second reagent tube from being sucked back into the first reagent tube and affecting the progress of the reaction.

In one embodiment, the reaction chamber comprises a main channel and a plurality of unit reaction chambers separated from each other, the unit reaction chambers are communicated with the main channel through branch pipelines, and the unit reaction chambers are used for placing the second reaction reagent. When the liquid enters from the opening of the main channel, the liquid can flow into each unit reaction chamber to react with the preset second reaction reagent. Different kinds of reaction reagents can be preset in different unit reaction chambers, and various requirements are met.

In one embodiment, the second reagent tube has a cylindrical shape, a rectangular parallelepiped shape, a spherical shape or an ellipsoidal shape, and may have an irregular shape, such as a blade shape in a longitudinal section.

In one embodiment, the test tube body is a plastic test tube body or a silica gel test tube body. The material of the test tube body can be plastic material or silica gel material, such as high polymer or composite high polymer such as polypropylene, polystyrene, etc. The test tube body is preferably made of soft materials, so that the reaction reagent in the test tube body can be conveniently extruded.

In one embodiment, the tube is a transparent tube. The transparent tube body can conveniently observe the color change inside, and judge the reaction process and the reaction result.

Compared with the prior art, the utility model has the following beneficial effects:

according to the reaction reagent tube, the first reagent tube and the second reagent tube are arranged, two reaction reagents are separately sealed and placed, when detection is needed, the sealing part of the first reagent tube is detached, the sealing film is punctured by the puncturing part, and then the reaction reagents in the test tube body flow into the tube body to contact and react with the second reaction reagents. The process is simple and easy to operate, the reaction reagent can be filled in advance according to the dosage, the operator can test without special training, and the operation success rate is high. Moreover, because the two reaction reagents are mixed only when in use, the two reaction reagents are put in place in one step, raw material or reaction pollution possibly generated in the operation process is greatly reduced, and the detection efficiency and accuracy are improved. In addition, the reaction equipment is miniaturized and operates quickly, and is favorable for realizing home self-testing, on-site screening and outpatient detection.

Drawings

FIG. 1 is a schematic structural diagram of a first reagent tube in the example.

FIG. 2 is a schematic structural view of a second reagent vessel in the example.

FIG. 3 is a schematic diagram of the structure of the reaction reagent tube in the example of carrying out the detection reaction.

Fig. 4 is a schematic structural view of the tube in embodiment 2.

100, a first reagent tube, 110, a test tube body, 111, a first external thread, 112, a first reaction reagent, 120, a puncture part, 121, a bulge part, 130, a sealing part, 200, a second reagent tube, 210, a sealing film, 220, a protective cover, 230, a second reaction reagent, 240, a reaction cavity, 241, a main channel, 242, a unit reaction chamber, 243 and a branch pipeline.

Detailed Description

To facilitate an understanding of the utility model, the utility model will now be described more fully with reference to the accompanying drawings. Preferred embodiments of the present invention are shown in the drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the utility model herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the utility model.

Example 1

A rapid test reaction reagent vessel, as shown in FIGS. 1 to 3, includes a first reagent vessel 100 and a second reagent vessel 200.

The first reagent tube 100 includes a test tube body 110, a puncture portion 120 and a sealing portion 130, the test tube body 110 is a slender structure, an accommodating cavity for accommodating the first reaction reagent 112 is provided in the test tube body 110, one end of the puncture portion 120 is fixedly connected to the test tube body 110, a through hole is provided in the puncture portion 120, one end of the through hole is communicated with the accommodating cavity, and the other end is communicated with the outside, that is, the first reaction reagent 112 in the accommodating cavity can flow into the through hole and flow out through the through hole. The sealing part 130 is detachably connected with one end of the puncture part 120 far away from the test tube body 110, when the sealing part 130 is installed on the puncture part 120, the through hole can be sealed, and when the test tube is normally placed, transported or stored, the first reaction reagent 112 cannot be discharged through the through hole and cannot leak; when the sealing portion 130 is not mounted on the piercing portion 120, the through hole communicates with the outside, and the first reactive agent 112 may flow out.

The second reagent tube 200 includes a tube body, a reaction chamber 240 with an opening at one end is disposed in the tube body, the reaction chamber 240 is used for placing the second reagent 230, a sealing film 210 is disposed at the opening of the tube body, the sealing film 210 seals the reaction chamber 240 to prevent the second reagent 230 from deteriorating due to contact with the outside, the sealing film 210 can be punctured by the puncturing part 120, and the first reagent 112 flows into the reaction chamber 240 and contacts with the second reagent 230 to react. The sealing film 210 is easily punctured, and a detachable protective cover 220 may be disposed outside the sealing film 210 and needs to be detached for detection or reaction.

The test tube body 110 and the puncture part 120 may be of an integrated structure, or may be in a threaded connection, in this embodiment, a threaded connection is adopted, specifically, a first external thread 111 is arranged at one end of the opening of the test tube body 110, and a first internal thread matched with the first external thread 111 is arranged on the puncture part 120. The threaded connection may facilitate loading of the cuvette body 110 with reagents or with a sample to be tested.

The sealing part 130 is detachably connected with the puncturing part 120, preferably in a threaded connection, specifically, a second external thread is provided on the puncturing part 120, and a second internal thread matched with the second external thread is provided on the sealing part 130. In order to facilitate puncturing the sealing film 210, one end of the puncturing part 120 away from the test tube body is a convex part 121, the convex part 121 is in a column shape with a wide top and a narrow bottom, the through hole penetrates through the convex part 121 to be communicated with the outside, and the second external thread is arranged on the convex part 121. The sealing part 130 is in threaded connection with the puncturing part 120, so that the sealing part 130 can be conveniently mounted and dismounted. Preferably, the puncturing part 120 is a screw cap with two open ends, one end of which has internal threads and the other end of which has external threads; the sealing part 130 is a plastic cap with internal threads.

The height of the protrusion 121 is set to be 0.2 to 0.5 times of the height of the reaction chamber 240, so that the reaction liquid in the second reagent tube 200 can be prevented from being sucked back into the first reagent tube 100, and the reaction progress can be prevented from being influenced.

In order to allow the reaction to proceed in a sealed environment, a third internal thread matching the second external thread is provided on the tube body, and the tube body may be screw-coupled to the piercing part 120. After the reaction solution in the first reagent tube 100 flows into the second reaction reagent 230, the piercing part 120 is screwed to the tube body, so that the reaction proceeds in a sealed environment and is prevented from being contaminated. For the convenience is extruded the first reaction liquid in test tube body 110, test tube body 110 adopts soft plastics material or silica gel material. In order to conveniently observe the color change inside the tube body and judge the reaction process and the reaction result, the tube body is made of transparent plastic.

Example 2

A rapid test reaction reagent tube, which is substantially the same as that in embodiment 1, except that the internal structure of the reaction chamber 240 is different, as shown in fig. 4, the reaction chamber 240 includes a main channel 241 extending downward, and a plurality of unit reaction chambers 242 distributed on both sides and below the main channel 241, each unit reaction chamber 242 is communicated with the main channel 241 through a branch channel 243, and each unit reaction chamber 242 can be preset with a reaction reagent. The unit reaction chambers 242 are preferably spherical.

When the liquid enters from the opening of the main channel 241, it can flow into each unit reaction chamber 242 to react with the preset reaction reagent, and this process can be realized by external force such as squeezing the first reagent tube 100 or centrifugation. The branch pipe 243 is inclined downward from an end connected to the main channel 241 to an end connected to the unit reaction chamber 242 to ensure smooth entry of the liquid into the unit reaction chamber 242. Different unit reaction chambers 242 may be pre-loaded with the same or different types of reagents to meet various detection requirements.

The shape of the second reagent vessel can be selected according to the requirement, such as cylindrical shape, flat rectangular parallelepiped shape (as shown in FIG. 4), spherical shape, and ellipsoidal shape, and irregular shape such as blade shape in longitudinal section can be adopted, as long as the second reagent vessel can achieve the above-mentioned function.

Example 3

This example provides an example of detection using the above-described rapid detection reaction reagent tube.

The first reaction reagent 112 is a nucleic acid releasing agent, the nucleic acid releasing agent is a solution prepared from Tris salt buffer solution, NP40 lysate, dNTP, NaCl, BSA and the like, the second reaction reagent 230 is a nucleic acid amplification reagent, and the reagent form includes but is not limited to liquid, gel, freeze-dried powder, freeze-dried spheres and the like. The nucleic Acid Amplification reagent can be a temperature-rising PCR reagent or a real-time fluorescent PCR reagent, including but not limited to a conventional PCR Amplification reagent, a real-time fluorescent PCR reagent, a nested PCR Amplification reagent, and can also be a constant-temperature based Amplification detection reagent, including but not limited to reagent components of LAMP (Loop-mediated Isothermal Amplification), RPA (recombinant Polymerase Amplification), NASBA (nucleic Acid Sequence-based Amplification), CRISPR (CRISPR) and other methods. Of course, the first reactive agent 112 and the second reactive agent 230 may be replaced with other biochemical or immunological reactive agents according to the needs and reaction needs of the user.

Detaching the puncture part 120, adding the sample to be tested into the nucleic acid releasing agent, fully mixing, mounting the puncture part 120, detaching the sealing part 130, detaching the protective cover 220, puncturing the sealing film 210 by using the puncture part 120, extruding the test tube body 110 to extrude the mixed solution into the tube body, contacting and reacting with the nucleic acid amplification reagent, placing the reaction reagent tube at 37-70 ℃ for reaction for 10-60min, and judging a negative or positive result according to the color change of the solution in the tube body.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the utility model. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims

1. A rapid assay reagent cartridge, comprising:

2. The tube for rapid test reaction according to claim 1, wherein the cuvette body and the piercing part are of an integrated structure.

3. The tube of claim 1, wherein the piercing part is threadedly coupled to the cuvette body.

4. The tube according to claim 3, wherein the test tube body has a first external thread at an end thereof adjacent to the piercing part, and the piercing part has a first internal thread matching the first external thread.

5. The tube of claim 1, wherein the sealing portion is threadedly coupled to the piercing portion.

6. The tube for rapid detection of a reaction reagent according to claim 5, wherein a protrusion is disposed at an end of the piercing part away from the test tube body, the protrusion is provided with a second external thread, and the sealing part is provided with a second internal thread matching the second external thread.

7. The tube according to claim 6, wherein a third internal thread is provided on the tube body to match the second external thread, and the tube body is threadably connected to the piercing portion.

8. The tube for rapid detection of a reaction reagent according to claim 6, wherein the height of the protrusion is 0.2 to 0.5 times the height of the reaction chamber.

9. The tube of claim 7, wherein the reaction chamber comprises a main channel and a plurality of unit reaction chambers separated from each other, the unit reaction chambers are communicated with the main channel through branch pipes, and the unit reaction chambers are used for placing the second reaction reagent.

10. The tube for rapid detection of a reaction reagent according to any one of claims 1 to 9, wherein the tube body is a plastic tube body or a silica gel tube body, and the tube body is a transparent tube body.