CN218404216U

CN218404216U - Nucleic acid detection connecting piece and nucleic acid amplification detection assembly

Info

Publication number: CN218404216U
Application number: CN202222579465.XU
Authority: CN
Inventors: 金伟
Original assignee: Guangna Dakang Guangzhou Biotechnology Co ltd
Current assignee: Guangna Dakang Guangzhou Biotechnology Co ltd
Priority date: 2022-09-28
Filing date: 2022-09-28
Publication date: 2023-01-31
Anticipated expiration: 2032-09-28

Abstract

The utility model discloses a nucleic acid detection connecting piece and a nucleic acid amplification detection assembly, wherein the nucleic acid detection connecting piece comprises a detection base, a drainage hole is arranged on the detection base, and a drainage unit is convexly arranged in the drainage hole; when the nucleic acid is detected, firstly, the inclusion to be detected is obtained through the detection unit, and then the detection unit is connected with the first baffle and the second baffle, so that the inclusion in the detection unit can be introduced into the detection test paper by the drainage unit, and the detection of the inclusion is completed; in the process, the first baffle and the second baffle can improve the whole sealing performance, and the second baffle and the connecting buckle thereof can improve the connection stability between the detection base and the detection unit; meanwhile, the drainage unit protruding out of the drainage hole can extend into the detection unit, so that the contact area of the inclusion and the drainage unit is as large as possible, and the detection rate is ensured; therefore, compared with the prior art, the nucleic acid detection connecting piece and the nucleic acid amplification detection assembly have improved sealing performance and detection rate.

Description

Nucleic acid detection connecting piece and nucleic acid amplification detection assembly

Technical Field

The utility model relates to a nucleic acid detects technical field, especially relates to a nucleic acid detects connecting piece and nucleic acid amplification determine module.

Background

In recent years, with the rapid development of molecular biology techniques, diagnostic methods based on nucleic acid detection have been widely established and widely applied to laboratory detection of human diseases, isothermal amplification technology is one of them, and isothermal amplification has the advantages of rapidness, high efficiency and specificity compared with other nucleic acid amplification technologies and does not need special equipment, so that it is considered by many scholars as a detection method possibly comparable to PCR once it appears. Currently, PCR molecular diagnostic technology is defined as the gold standard for detection, but the requirement for a thermal cycler is high, and the detection is not favorable for field and primary hospital detection. Thus, scientists have attempted to use alternative exponential amplification techniques, such as loop-mediated isothermal amplification (LAMP), recombinase polymerase amplification, hyperbranched rolling circle amplification, exponential amplification reactions, and exponential strand displacement amplification, among others. The exponential amplification technology reacts under the constant temperature condition without thermal cycle, thereby not only expanding the application scene of the nucleic acid detection technology, but also exciting the potential of POCT application. The molecular diagnosis has the characteristics of high sensitivity and strong specificity, and if the rapid advantage of POCT is further integrated, the molecular diagnosis inevitably occupies an important position in the detection field.

POCT refers to a detection mode which is carried out on a sampling site and utilizes a portable analysis instrument and a matched reagent to quickly obtain a detection result. POCT meaning can be understood from two aspects: spatially, tests performed at the patient's side, i.e. "bedside tests"; temporally, a "point-of-care" test can be performed.

The nucleic acid amplification product produced based on the constant temperature amplification technology can perform single molecule nucleic acid detection at a constant and low temperature within 30 min. The current nucleic acid amplification products in China have low requirements on environment and hardware facilities, and have good application in the aspects of biological protection, water body inspection, food inspection, medical diagnosis, microfluid, veterinarian and the like; however, the current nucleic acid amplification products are subject to the following situations: when the sealing property needs to be increased in order to ensure that the amplification product does not leak, the internal pressure of the amplification product is correspondingly increased, so that the liquid is prevented from smoothly flowing into a space for detection, and the detection speed is too slow; similarly, when the detection rate is increased, the sealing performance is also decreased.

Namely, the nucleic acid amplification product in the prior art has the technical problem that the sealing performance and the detection rate are incompatible.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a nucleic acid detects connecting piece and nucleic acid amplification determine module, solve the nucleic acid amplification class product among the prior art at present and have both incompatible technical problem of leakproofness and detection rate.

To achieve the purpose, the utility model adopts the following technical proposal:

a nucleic acid detection connecting piece comprises a detection base, wherein a detection cavity for placing detection test paper is formed in the detection base;

a drainage hole is formed in the wall of the detection cavity, a first baffle and a second baffle for connecting a detection unit are arranged on the detection base in an outward protruding mode, and a connecting buckle is arranged on the second baffle in a protruding mode in the direction close to the drainage hole;

and a drainage unit is inserted into the drainage hole, one end of the drainage unit protrudes out of the drainage hole and is used for introducing the inclusion in the detection unit, and the other end of the drainage unit is used for guiding the inclusion to the detection test paper.

Optionally, the first baffle and the second baffle are both annular;

the first baffle with the second baffle distribute in outside the drainage hole, just the second baffle set up in first baffle keeps away from one side in drainage hole.

Optionally, a plurality of first notches are formed in the first baffle plate along the circumferential direction of the first baffle plate, and a plurality of second notches are formed in the second baffle plate along the circumferential direction of the second baffle plate.

Optionally, a protruding portion is further arranged on the detection base in an outward protruding manner, an avoidance hole is formed in the protruding portion, and the avoidance hole is communicated with the drainage hole; one end of the drainage unit protrudes out of the avoiding hole.

Optionally, the detection base comprises an upper detection shell and a lower detection shell;

the detection upper shell is provided with a detection upper groove, a first upper buckle is convexly arranged at the bottom of the detection upper groove, and a second upper buckle is convexly arranged at the wall of the detection upper groove;

the lower detection shell is provided with a lower detection groove, a first lower buckle is convexly arranged at the bottom of the lower detection groove, and a second lower buckle is convexly arranged at the edge of the notch of the lower detection groove;

the detection upper groove and the detection lower groove are in butt-joint surrounding arrangement to form the detection cavity, the first upper buckle is connected with the first lower buckle in a clamping manner, and the second upper buckle is connected with the second lower buckle in a clamping manner.

Optionally, the lower detection shell is provided with a sealing groove at the edge of the notch of the lower detection groove;

the upper detection shell is provided with a sealing block in a protruding mode corresponding to the sealing groove, and the sealing block is sleeved in the sealing groove.

Optionally, a plurality of water absorption mounting blocks are arranged on the bottom cavity wall of the detection cavity corresponding to the drainage holes;

the water absorption installation blocks are distributed in a circumferential mode, and the inner sides of the water absorption installation blocks are clamped with water absorption units.

Optionally, a third baffle arranged along the length direction of the test paper is arranged on the bottom cavity wall of the detection cavity, a plurality of fourth baffles arranged at intervals are further arranged on the bottom cavity wall of the detection cavity, the height of the third baffle is lower than that of the fourth baffles, and the third baffle sequentially penetrates through the plurality of fourth baffles.

A nucleic acid amplification detection assembly comprising a detection unit and a nucleic acid detection linker as described above; the detection unit is detachably connected with the nucleic acid detection connecting piece.

Optionally, the detection unit comprises an amplification unit and a dilution unit which can be communicated with each other, the amplification unit is used for storing amplification reagents and nucleic acid samples, and the dilution unit is used for storing a dilution liquid;

the dilution unit with amplification unit detachably connects, just the dilution unit with be provided with the seal membrane between the amplification unit, dilution unit swing joint has and is used for puncturing the puncture unit of seal membrane, the amplification unit includes flexible amplification casing.

Compared with the prior art, the utility model discloses following beneficial effect has:

the utility model provides a nucleic acid detection connecting piece and nucleic acid amplification detection subassembly, when carrying out nucleic acid detection, obtain the inclusion that needs to detect through the detecting element earlier, then be connected detecting element and first baffle and second baffle for the drainage unit can introduce the detection test paper with the inclusion in the detecting element, thereby accomplish the detection to the inclusion; in the process, the first baffle and the second baffle can improve the whole sealing performance, and the second baffle and the connecting buckle thereof can improve the connection stability between the detection base and the detection unit; meanwhile, the drainage unit protruding out of the drainage hole can extend into the detection unit, so that the contact area of the inclusion and the drainage unit is as large as possible, and the detection rate is ensured; therefore, compared with the prior art, the nucleic acid detection connecting piece and the nucleic acid amplification detection assembly have improved sealing performance and detection rate.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without inventive exercise.

The structure, proportion, size and so on shown in the drawings of the present specification are only used to cooperate with the content disclosed in the specification for the understanding and reading of the people skilled in the art, and are not used to limit the limit conditions of the present invention, so that the present invention does not have the essential significance in the technology, and any structure modification, proportion relation change or size adjustment should still fall within the scope covered by the technical content disclosed in the present invention without affecting the function and the achievable by the present invention.

FIG. 1 is a schematic diagram of an overall structure of a nucleic acid detecting connector according to an embodiment of the present invention;

FIG. 2 is a schematic cross-sectional view of a nucleic acid detecting connector according to an embodiment of the present invention;

fig. 3 is a schematic view of an overall structure of the upper detection shell according to the first embodiment of the present invention;

fig. 4 is a schematic view of an overall structure of a lower detection shell according to a first embodiment of the present invention;

fig. 5 is a schematic structural diagram of a detection unit according to a second embodiment of the present invention;

FIG. 6 is a schematic structural diagram of a nucleic acid amplification detecting assembly according to the second embodiment of the present invention.

Illustration of the drawings: 10. detecting a base; 11. a detection cavity; 12. a drainage hole; 131. a first baffle; 132. a second baffle; 133. connecting a buckle; 14. a boss portion; 15. a water absorbing mounting block; 16. an observation window; 20. detecting test paper; 30. a detection unit; 40. a drainage unit;

50. detecting the upper shell; 51. detecting the upper groove; 52. a first upper buckle; 53. a second upper buckle; 54. a sealing block; 55. a fifth baffle; 56. a sixth baffle; 57. a seventh baffle; 58. an eighth baffle;

60. detecting the lower shell; 61. detecting a lower groove; 62. a first lower buckle; 63. a second lower buckle; 64. a sealing groove; 65. a third baffle; 66. a fourth baffle;

70. an amplification unit; 71. an amplification chamber; 80. a dilution unit; 81. a sealing film; 82. a dilution cavity; 90. and a puncturing unit.

Detailed Description

To make the objects, features and advantages of the present invention more obvious and understandable, the embodiments of the present invention are clearly and completely described in the embodiments of the present invention with reference to the drawings, and obviously, the embodiments described below are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

In the description of the present invention, it is to be understood that the terms "upper", "lower", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention. It should be noted that when one component is referred to as being "connected" to another component, it can be directly connected to the other component or intervening components may also be present.

The technical solution of the present invention is further explained by the following embodiments with reference to the accompanying drawings.

Please refer to fig. 1 to fig. 6, fig. 1 is an overall structure schematic diagram of a nucleic acid detecting connecting piece provided by the first embodiment of the present invention, fig. 2 is a sectional structure schematic diagram of a nucleic acid detecting connecting piece provided by the first embodiment of the present invention, fig. 3 is an overall structure schematic diagram of a detecting upper shell body of the first embodiment of the present invention, fig. 4 is an overall structure schematic diagram of a detecting lower shell body of the first embodiment of the present invention, fig. 5 is a structural schematic diagram of a detecting unit of the second embodiment of the present invention, fig. 6 is a structural schematic diagram of a nucleic acid amplification detecting assembly provided by the second embodiment of the present invention.

Example one

The nucleic acid detecting connecting piece provided by the embodiment can be connected with a detecting unit 30 for storing the content (such as the nucleic acid amplification product) so as to detect the content, wherein the structure of the nucleic acid detecting connecting piece is optimized, so that the sealing performance and the detection rate of the nucleic acid detecting connecting piece are improved.

As shown in FIG. 1 and FIG. 2, the nucleic acid detecting connector of the present embodiment includes a detecting base 10, and a detecting cavity 11 for placing a detecting test paper 20 is formed in the detecting base 10. Meanwhile, the detection base 10 is provided with an observation port, an observation window 16 is embedded in the observation port, and the surface of the observation window 16 can be covered with a transparent sealing layer to increase the sealing performance of the nucleic acid detection connecting piece.

A drainage hole 12 is formed in the wall of the detection cavity 11, a first baffle 131 and a second baffle 132 for connecting the detection unit 30 are arranged on the detection base 10 in an outward protruding manner, and a connecting buckle 133 is arranged on the second baffle 132 in a protruding manner along the direction close to the drainage hole 12; it should be understood that the connection buckle 133 protrudes inward, which can increase the stability of the connection between the nucleic acid detection connection member and the detection unit 30 during detection, so that the detection unit 30 cannot easily fall off during the detection process, and the leakage of the nucleic acid amplification product is effectively prevented from polluting the environment.

A drainage unit 40 is inserted into the drainage hole 12, one end of the drainage unit 40 protrudes out of the drainage hole 12 and is used for introducing the contents in the detection unit 30, and the other end of the drainage unit 40 is used for guiding the contents to the detection test paper 20. Wherein, the drainage unit 40 can be made of water-absorbing material; it should be understood that, since the drainage unit 40 protrudes from the drainage hole 12, that is, can extend into the detection unit 30, the contact area between the liquid to be detected and the drainage unit 40 is increased, thereby improving the detection efficiency.

Specifically, when detecting nucleic acid, firstly, the detecting unit 30 obtains the content to be detected, and then the detecting unit 30 is connected to the first baffle 131 and the second baffle 132, so that the drainage unit 40 can introduce the content in the detecting unit 30 into the detection test paper 20, thereby completing the detection of the content; in this process, the first baffle 131 and the second baffle 132 can both improve the overall sealing performance, and the second baffle 132 and the connecting buckle 133 thereof can improve the connection stability between the detection base 10 and the detection unit 30; meanwhile, the drainage unit 40 protruding out of the drainage hole 12 can extend into the detection unit 30, so that the contact area of the contents and the drainage unit 40 is as large as possible, thereby ensuring the detection rate; therefore, compared with the prior art, the nucleic acid detection connecting piece and the nucleic acid amplification detection assembly have the advantages that the tightness and the detection rate are improved.

In a particular embodiment, the first baffle 131 and the second baffle 132 are both annular; first baffle 131 and second baffle 132 distribute outside drainage hole 12, and second baffle 132 sets up in one side that drainage hole 12 was kept away from to first baffle 131. In other alternative embodiments, the first blocking plate 131 and the second blocking plate 132 may be made of other structures such as a strip shape, which can meet the connection requirements of other types of detecting units 30. The first baffle 131 and the second baffle 132 may be attached or not.

On the basis of the above embodiment, the first baffle 131 is provided with a plurality of first notches along the circumferential direction thereof, and the second baffle 132 is provided with a plurality of second notches along the circumferential direction thereof, so that the situation that the baffles are blocked when being connected with the detection unit 30 can be avoided.

Further, as shown in fig. 1 and 2, a protruding portion 14 is further disposed on the detection base 10 and protrudes outward, and an avoiding hole is formed in the protruding portion 14 and is communicated with the drainage hole 12; one end of the drainage unit 40 protrudes from the avoidance hole.

Further, as shown in fig. 3 and 4, the inspection base 10 includes an inspection upper case 50 and an inspection lower case 60.

The detection upper shell 50 is provided with a detection upper groove 51, a first upper buckle 52 is convexly arranged at the bottom of the detection upper groove 51, and a second upper buckle 53 is convexly arranged on the wall of the detection upper groove 51; the lower detection shell 60 is provided with a lower detection groove 61, the bottom of the lower detection groove 61 is convexly provided with a first lower buckle 62, and the edge of the notch of the lower detection groove 61 is convexly provided with a second lower buckle 63; illustratively, the first upper buckle 52 is a cylindrical structure, the first lower buckle 62 is a cylindrical structure provided with a square groove, and the first upper buckle 52 can be inserted into the square groove in the first lower buckle 62; the second upper buckle 53 is a clamping block, the second lower buckle 63 is a clamping groove, and the clamping block can be clamped in the clamping groove.

The upper detection groove 51 and the lower detection groove 61 are arranged in an abutting and surrounding manner to form the detection cavity 11, the first upper buckle 52 is connected with the first lower buckle 62 in a clamping manner, and the second upper buckle 53 is connected with the second lower buckle 63 in a clamping manner. Because the first buckle and the second buckle are arranged in the detection cavity 11, the overall sealing performance can be improved, the pressure formed outside the detection base 10 is avoided, the deformation of the detection base 10 is reduced, and the sealing performance is further improved.

Further, a sealing groove 64 is formed in the lower detection shell 60 at the edge of the notch of the lower detection groove 61; the upper detection shell 50 is convexly provided with a sealing block 54 corresponding to the sealing groove 64, and the sealing block 54 is sleeved in the sealing groove 64. The flanges (specifically, the first flange located on the inner side and the second flange located on the outer side) of the upper detection shell 50 on both sides of the sealing block 54 respectively abut against the edges (specifically, the first edge located on the inner side and the second edge located on the outer side) of the lower detection shell 60 on both sides of the notch of the sealing groove 64, so that the sealing performance is further improved. The second edge may be higher than the first edge, and accordingly, the upper detection housing 50 and the lower detection housing 60 may form a snap-fit function, thereby further improving the sealing property.

Further, as shown in fig. 4, a plurality of water absorption mounting blocks 15 are arranged on the bottom cavity wall of the detection cavity 11 corresponding to the drainage holes 12; the plurality of water absorption mounting blocks 15 are circumferentially distributed, and water absorption units are clamped on the inner sides of the plurality of water absorption mounting blocks 15. The water absorption unit is installed in the mode, so that cost and process can be saved, and the water absorption unit is made of water absorption materials and plays a role in absorbing redundant inclusions, so that the phenomenon of flushing is prevented.

Further, as shown in fig. 4, a third baffle 65 is disposed on the bottom cavity wall of the detection cavity 11 along the length direction of the detection test paper 20, a plurality of fourth baffles 66 are disposed on the bottom cavity wall of the detection cavity 11 at intervals, the height of the third baffle 65 is lower than that of the fourth baffles 66, and the third baffle 65 sequentially passes through the plurality of fourth baffles 66. The fourth baffle 66 can lift the detection test paper 20, so that the detection test paper 20 is far away from the bottom wall of the detection cavity 11, thereby preventing the occurrence of the flushing phenomenon. Meanwhile, the third baffle 65 and the fourth baffle 66 are disposed at the bottom of the detection lower tank 61.

Further, as shown in fig. 3, the upper detection tank 51 is further provided with a fifth baffle 55, a sixth baffle 56, a seventh baffle 57 and an eighth baffle 58; the protruding portion 14 further extends in a direction close to the detection cavity 11, and the fifth baffle 55 extends from the outer wall of the protruding portion 14 to fix the detection test paper 20; the sixth and

eighth barriers

56 and 58 serve to fix the test strip 20 perpendicularly to the extending direction of the test strip 20, wherein the eighth barrier 58 is used to fix the end of the test strip 20. The seventh baffle 57 is provided with a limiting portion protruding from two sides of the test paper 20, so as to limit the test paper 20.

In conclusion, the nucleic acid detecting connecting piece provided by the embodiment has the advantages of improved sealing performance and detection rate, low cost, stable structure and the like compared with the prior art.

Example two

As shown in FIGS. 5 and 6, the nucleic acid amplification detecting assembly of the present embodiment includes a detecting unit 30 and a nucleic acid detecting connector of the first embodiment; the detection unit 30 is detachably connected to the nucleic acid detecting linker. Here, the structure of the sensing unit 30 is not particularly limited, and it is sufficient that the contents can be placed. In the first embodiment, the specific structure and technical effects of the nucleic acid detecting connector are described, and the nucleic acid amplification detecting module of the present embodiment also has the technical effects.

Illustratively, the detecting unit 30 includes an amplifying unit 70 and a diluting unit 80 which are communicated with each other, the amplifying unit 70 is used for storing an amplifying reagent and a nucleic acid sample, and the diluting unit 80 is used for storing a diluting solution; wherein, an amplification cavity 71 is formed in the amplification unit 70, the amplification cavity 71 is configured with a first opening, and a nucleic acid sample and an amplification reagent can be put into the amplification cavity 71 from the first opening; a dilution cavity 82 is formed in the dilution unit 80 and comprises a second opening and a third opening, the second opening can be communicated with the first opening, the sealing film 81 is arranged on the second opening, and the third opening is movably connected with a puncture unit 90 for puncturing the sealing film 81; the dilution unit 80 is detachably connected to the amplification unit 70 with a sealing film 81 disposed between the dilution unit 80 and the amplification unit 70, and the amplification unit 70 includes a flexible amplification housing.

Specifically, a nucleic acid sample and an amplification reagent may be put into the amplification cavity 71 through the first opening, and the amplification unit 70 and the dilution unit 80 are connected to each other until the amplification reaction is finished; pushing the puncture unit 90 for the first time to break the sealing film 81, thereby completing the dilution of the amplification product and obtaining the inclusion; then, the detection unit 30 is mounted on the detection base 10, the drainage unit 40 further pushes the puncture unit 90, so that the drainage unit 40 enters the detection unit 30, and the contents are introduced into the detection cavity 11, so as to complete the detection of the contents. In the above process, since the amplification unit 70 includes a flexible amplification housing, the operator can change the internal pressure by pinching the flexible amplification housing, so that the contents flow into the detection chamber 11, and it is understood that, when the sealing property of the detection unit 30 is improved by other means, the contents can flow in the above manner, so that the situation that the contents are not easily flowed to the nucleic acid detection linker due to too high pressure is avoided; the puncturing unit 90 can be indirectly moved by pinching and flexibly amplifying the housing, so that the puncturing unit 90 is displaced, and the puncturing unit 90 is moved in a direction away from the drainage unit 40, thereby increasing the contact area between the contents and the drainage unit 40 and further improving the detection efficiency.

In summary, the nucleic acid amplification detecting assembly provided by the embodiment has improved sealing performance and detection rate compared with the prior art, and has the advantages of low cost, stable structure and the like.

The above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention in its corresponding aspects.

Claims

1. The nucleic acid detection connecting piece is characterized by comprising a detection base (10), wherein a detection cavity (11) for placing detection test paper (20) is formed in the detection base (10);

a drainage hole (12) is formed in the wall of the detection cavity (11), a first baffle (131) and a second baffle (132) which are used for connecting a detection unit (30) are arranged on the detection base (10) in an outward protruding mode, and a connecting buckle (133) is arranged on the second baffle (132) in a protruding mode in the direction close to the drainage hole (12);

a drainage unit (40) is inserted into the drainage hole (12), one end of the drainage unit (40) protrudes out of the drainage hole (12) and is used for introducing the content in the detection unit (30), and the other end of the drainage unit (40) is used for guiding the content to the detection test paper (20).

2. The nucleic acid detecting link according to claim 1, wherein the first flap (131) and the second flap (132) are each annular;

the first baffle (131) and the second baffle (132) are distributed outside the drainage holes (12), and the second baffle (132) is arranged on one side, away from the drainage holes (12), of the first baffle (131).

3. The nucleic acid detecting connecting member according to claim 2, wherein the first flap (131) has a plurality of first notches formed along a circumferential direction thereof, and the second flap (132) has a plurality of second notches formed along a circumferential direction thereof.

4. The nucleic acid detection connecting piece according to claim 1, wherein the detection base (10) is further provided with a protruding portion (14) protruding outwards, the protruding portion (14) is provided with an avoiding hole, and the avoiding hole is communicated with the drainage hole (12); one end of the drainage unit (40) protrudes out of the avoidance hole.

5. The nucleic acid detecting attachment according to claim 1, wherein the detection base (10) includes a detection upper case (50) and a detection lower case (60);

the detection upper shell (50) is provided with a detection upper groove (51), the bottom of the detection upper groove (51) is convexly provided with a first upper buckle (52), and the wall of the detection upper groove (51) is convexly provided with a second upper buckle (53);

the detection lower shell (60) is provided with a detection lower groove (61), the bottom of the detection lower groove (61) is convexly provided with a first lower buckle (62), and the edge of the notch of the detection lower groove (61) is convexly provided with a second lower buckle (63);

the detection upper groove (51) and the detection lower groove (61) are arranged in a surrounding mode to form the detection cavity (11), the first upper buckle (52) is connected with the first lower buckle (62) in a clamping mode, and the second upper buckle (53) is connected with the second lower buckle (63) in a clamping mode.

6. The nucleic acid detecting connecting piece according to claim 5, wherein the detecting lower case (60) is provided with a sealing groove (64) at the edge of the notch of the detecting lower groove (61);

the upper detection shell (50) is provided with a sealing block (54) corresponding to the sealing groove (64) in a protruding mode, and the sealing block (54) is sleeved in the sealing groove (64).

7. The nucleic acid detection connecting piece according to claim 1, wherein the bottom cavity wall of the detection cavity (11) is provided with a plurality of water absorption mounting blocks (15) at positions corresponding to the drainage holes (12);

the water absorption installation blocks (15) are distributed in a circumferential mode, and water absorption units are clamped on the inner sides of the water absorption installation blocks (15).

8. The nucleic acid detection connecting piece according to claim 1, characterized in that a bottom cavity wall of the detection cavity (11) is provided with a third baffle (65) arranged along the length direction of the detection test paper (20), the bottom cavity wall of the detection cavity (11) is further provided with a plurality of fourth baffles (66) arranged at intervals, the height of the third baffle (65) is lower than that of the fourth baffles (66), and the third baffle (65) sequentially penetrates through the plurality of fourth baffles (66).

9. A nucleic acid amplification detection assembly comprising a detection unit (30) and a nucleic acid detection linker according to any one of claims 1 to 8; the detection unit (30) is detachably connected to the nucleic acid detecting attachment.

10. The nucleic acid amplification detection assembly of claim 9, wherein the detection unit (30) comprises an amplification unit (70) and a dilution unit (80) which are communicated with each other, the amplification unit (70) is used for storing amplification reagents and nucleic acid samples, and the dilution unit (80) is used for storing a dilution liquid;

dilute unit (80) with increase unit (70) detachably connected, just dilute unit (80) with be provided with sealing membrane (81) between increase unit (70), dilute unit (80) swing joint has and is used for puncturing puncture unit (90) of sealing membrane (81), increase unit (70) including flexible amplification casing.