KR102182376B1 - Integrated molecular diagnosis cartridge - Google Patents

Abstract

The integrated molecular diagnosis cartridge for on-site diagnosis includes a block unit to which a plurality of cartridges are fixed, and an extraction unit connected to the cartridge to perform a pretreatment process for a sample. The block portion includes an intermediate block in which a plurality of holes into which the cartridges are respectively inserted, an upper block coupled to an upper portion of the intermediate block and aligned with the holes to form an upper space into which the extraction unit is inserted, and the intermediate block It is coupled to the lower portion and includes a lower block in which an amplification chamber for receiving a sample of which the pretreatment has been completed is formed.

Description

Low-power all-in-one molecular diagnosis cartridge for on-site diagnosis {INTEGRATED MOLECULAR DIAGNOSIS CARTRIDGE}

The present invention relates to a molecular diagnostic cartridge, and more specifically, a low-power integrated molecular diagnostic cartridge for on-site diagnostics configured to be operated with low power, so that direct diagnosis can be easily performed by a non-specialist in a medical outlying area or a medical field requiring rapid diagnosis It is about.

Molecular diagnosis technology is used in various ways for analysis and diagnosis purposes, such as detecting genetic diseases or diagnosing infectious diseases in the bio field.

Devices for such molecular diagnosis are generally designed to include relatively complex configurations, such as requiring various reagents and applying a pump or valve structure for driving them.

However, the device including such a complex configuration can improve the accuracy of the test result, but it is difficult for non-professionals to handle, and there is a hassle of collecting a sample to be analyzed and moving it to the analysis site. Accordingly, there is a problem of increasing the time and cost of analysis. That is, in the case of a conventional device for molecular diagnosis, it is difficult to perform an inspection using the device directly in the field.

Accordingly, in recent years, the molecular diagnosis technology has been developed in a direction to improve the usability for on-site diagnosis, and efforts have been made to be miniaturized and configured or to integrate all systems.

Korean Patent Registration No. 10-1244467 discloses a sample pretreatment cartridge capable of small, integrated, and continuous treatment of a field diagnosis type in connection with such molecular diagnosis technology. However, a complex structure is still employed, and since it includes a complex usage for use by non-professionals, there is a limit that is unsuitable for field diagnosis.

Korean Patent Registration No. 10-1244467

Therefore, the technical problem of the present invention is conceived in this respect, and the object of the present invention is to improve usability because it can be easily used by non-professionals in the medical field, can be driven with low power, and is configured as an integral type, so that the reliability of the test result is high. It relates to a low-power integrated molecular diagnosis cartridge for field diagnosis.

A molecular diagnostic cartridge according to an embodiment for realizing the object of the present invention includes a block portion in which a plurality of cartridges are fixed, and an extraction portion connected to the cartridge to perform a pretreatment process for a sample. The block portion includes an intermediate block in which a plurality of holes into which the cartridges are respectively inserted, an upper block coupled to an upper portion of the intermediate block and aligned with the holes to form an upper space into which the extraction unit is inserted, and the intermediate block It is coupled to the lower portion and includes a lower block in which an amplification chamber for receiving a sample of which the pretreatment has been completed is formed.

In one embodiment, the cartridges may include a dissolution cartridge for dissolving the sample, a cleaning cartridge for cleaning the sample, and an elution cartridge for dissolving the sample.

In one embodiment, an amplification module further comprises a heating unit for heating the pretreated sample of the amplification chamber under the amplification chamber, and a detection unit connected to the heating unit to duplicate and detect the nucleic acid template of the sample. Can include.

In one embodiment, the amplification chamber may further include an external chamber that is attached to and attached to the amplification chamber and provides the cloned nucleic acid template to the outside.

In one embodiment, the extraction unit, an extension portion sequentially inserted into each of the cartridges, a handle portion for sucking and discharging the solution of the cartridge to the extension portion, and the sample and the solution is collected by being inserted into the extension portion It may include a filter unit.

In one embodiment, the extraction unit may further include a guide block that moves along the extension and fixes a position of the extraction unit in the vertical direction on the upper space.

In one embodiment, the cartridges may be inserted into the intermediate block along the circumferential direction.

In one embodiment, the upper block may be coupled to be rotatable with respect to the middle block, and the middle block may be coupled to be rotatable with respect to the lower block.

In one embodiment, as the upper block is rotated with respect to the intermediate block, the upper space may be aligned with any one of the cartridges.

In one embodiment, the sample for which the pretreatment has been completed may be provided to the amplification chamber as the intermediate block is rotated with respect to the lower block.

In one embodiment, the cartridges may be inserted into the intermediate block along one direction.

In one embodiment, the extraction unit may move in the upper space and be aligned with any one of the cartridges.

In one embodiment, the lower block may include a lower hole connecting an end hole of the hole formed in the middle block and the amplification chamber.

According to embodiments of the present invention, on the block portion divided into upper, middle and lower blocks, the extraction portion is inserted through the upper block, cartridges for pretreatment of the sample are sequentially positioned in the middle block, and the lower block Finally, the pre-processed sample is provided and configured so that it can be replicated, making it possible to construct a molecular diagnostic cartridge with a relatively simple configuration, and improved assembly and user usability, resulting in improved portability and easy on-site diagnosis. Can be used.

In particular, the extraction unit is inserted or moved in the upper block, and the extraction unit is composed of a fluid mixing unit, and the mixing unit can be configured in various forms such as a dropper, a syringe, and a pressure/negative pressure pump, so that the user can easily handle it. It is configured to replicate the nucleic acid template through the amplification module in the lower block, so that all pre-processing for molecular diagnosis and all processes of nucleic acid analysis can be performed through one cartridge.

In addition, the block portion has a cylindrical shape, the upper block rotates with respect to the middle block, and the middle block rotates with respect to the lower block. Through this, process steps for each pre-treatment can be performed. Can be done.

In this case, the upper space on the upper block is located in a line according to the order of each process with the cartridges of the intermediate block, and each process can be easily performed through the extraction unit when aligned at the corresponding location, so that diagnosis speed and stability Diagnosis can be performed. In addition, it is possible to perform various pretreatment processes according to the type of sample by replacing the cartridge according to the required process.

On the other hand, on the cartridges arranged in a line in one direction, the extraction unit is sequentially moved and inserted so that each process can be performed continuously, so that only the rotational structure and the detailed operation method as described above are different. Diagnosis speed and stable diagnosis can be performed with simple operation.

However, in this case, by restricting the movement of the extraction unit in the vertical direction through the guide block, it is possible to prevent a problem due to the outflow of the sample.

1 is a schematic diagram showing a molecular diagnostic cartridge according to an embodiment of the present invention.
2A to 2E are schematic diagrams showing each step of the operation of the molecular diagnostic cartridge of FIG. 1.
3A to 3E are schematic diagrams specifically showing the operating state of the molecular diagnostic cartridge in FIGS. 2A and 2B.
4 is a schematic diagram showing a molecular diagnostic cartridge according to another embodiment of the present invention.
5A to 5E are schematic diagrams showing each step of the operation of the molecular diagnostic cartridge of FIG. 4.

The present invention will be described in detail in the text, since various modifications can be made and various forms can be obtained. However, this is not intended to limit the present invention to a specific form of disclosure, it is to be understood as including all changes, equivalents, or substitutes included in the spirit and scope of the present invention. In describing each drawing, similar reference numerals have been used for similar elements. Terms such as first and second may be used to describe various components, but the components should not be limited by the terms.

These terms are used only for the purpose of distinguishing one component from another component. The terms used in the present application are only used to describe specific embodiments, and are not intended to limit the present invention. Singular expressions include plural expressions unless the context clearly indicates otherwise.

In the present application, terms such as "comprise" or "consist of" are intended to designate the presence of features, numbers, steps, actions, elements, parts, or a combination thereof described in the specification, but one or more other features. It is to be understood that the presence or addition of elements or numbers, steps, actions, components, parts, or combinations thereof, does not preclude in advance the possibility of the presence or addition.

Unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which the present invention belongs. Terms as defined in a commonly used dictionary should be interpreted as having a meaning consistent with the meaning in the context of the related technology, and should not be interpreted as an ideal or excessively formal meaning unless explicitly defined in this application. Does not.

Hereinafter, preferred embodiments of the present invention will be described in more detail with reference to the accompanying drawings.

1 is a schematic diagram showing a molecular diagnostic cartridge according to an embodiment of the present invention.

Referring to FIG. 1, the molecular diagnostic cartridge 10 according to the present embodiment can be used for on-site diagnosis, that is, portable, in a medical field, can be used for single use, and can be manufactured in a relatively small size.

More specifically, the molecular diagnosis cartridge 10 includes a block unit 100, an extraction unit 200, a cartridge 300, an amplification module 400, and an external chamber 500.

First, the extraction part 200 includes an extension part 210 extending in one direction, a handle part 220 that is expanded while forming a predetermined space at one end of the extension part 210, and the extension part 210 ) And a filter unit 230 located inside.

As shown, the extraction unit 200 may have a shape of, for example, a dropper, and further, although not shown, may have various shapes such as a syringe, a pressure/negative pressure pump, and the like.

Thus, the user presses the handle part 220 to cause an external solution to flow into the inside of the extension part 210, that is, to the filter part 230 located in the extension part 210, or otherwise , It may be operated to discharge a solution located inside the extension part 210 to the outside.

In this case, the extraction unit 200 may be formed in various shapes of droppers, provided that the above-described operation can be performed in addition to the illustrated one. In this case, in addition to the shape of the dropper, it may be formed in a variety of shapes capable of performing mixing and reaction by pressurizing and negative pressure fluid such as a syringe or a pump.

In addition, the inflow and outflow operations through the handle part 220 may be repeatedly performed until the inflow and outflow of the solution is completely performed.

On the other hand, the extraction unit 200, although not shown, through a separate sample extraction step, in a state in which the sample is extracted by the filter unit 230, the sample using the molecular diagnosis cartridge 10 in this embodiment The pretreatment process can be performed.

In this case, the sample is extracted on the filter unit 230 and the pretreatment process is performed to amplify the viral nucleic acid template, and may be, for example, blood, sputum, feces, epithelial cells, or the like.

The block unit 100 includes an upper block 110, an intermediate block 120, and a lower block 130.

In this embodiment, the block unit 100 may have a cylindrical shape as a whole, and the upper block 110 is coupled to be rotatable with respect to the intermediate block 120, and likewise the intermediate block 120 It is coupled to be rotatable with respect to the lower block 130.

In this case, the shape of each block of the block unit 100 is not necessarily limited to a cylindrical shape, but the blocks must be coupled to be able to rotate relative to each other with respect to a central axis.

The upper block 110 includes an upper space 111 having a cylindrical hole shape, and on the upper surface of the upper block 110, an operation state of the molecular diagnostic cartridge 10 is displayed. The display unit 112 is displayed.

In the intermediate block 120, a plurality of holes 121, 122, 123, 124 are arranged at predetermined intervals in a circumferential direction around a central axis, and in each of the holes 121, 122, 123, 124, Each of the cartridges 310, 320, 330, 340 is inserted and fixed.

That is, the cartridge 300 includes a plurality of cartridges 310, 320, 330, and 340, and each of the cartridges includes solutions for performing each process of pretreatment for the sample.

For example, the first cartridge 310 is a dissolution cartridge that primarily dissolves the sample, the second cartridge 320 is a first cleaning cartridge that cleans the sample, and the third cartridge 330 is the A second cleaning cartridge for additionally cleaning a sample, and the fourth cartridge 340 may be an elution cartridge for performing elution on the sample.

Further, in addition to the exemplified pretreatment process, if an additional process is required, the first to fourth cartridges may be replaced with other types of cartridges, or the hole may be additionally formed to provide additional cartridges. .

Meanwhile, in a state in which a plurality of holes are formed on the intermediate block 120 and cartridges 310, 320, 330, and 340 for performing different pretreatment processes are fixed to each of the holes, the upper block ( 110) is rotated with respect to the intermediate block 120, through which each pretreatment process is performed.

A step of performing the process of the molecular diagnostic cartridge 10 will be described later.

The lower block 130 is disposed under the intermediate block 120 and may have a cylinder shape having the same radius as the intermediate block 120.

A lower hole part 131 is formed inside the lower block 130, and an amplification chamber 132 and the amplification module 400 are disposed.

The amplification chamber 132 forms a predetermined chamber space, may be formed to be sealed, and is connected to the intermediate block 120 through the lower hole part 131.

That is, the lower hole part 131 connects the fourth hole 124 of the intermediate block 120, that is, the fourth cartridge 340 and the amplification chamber 132, and connects the intermediate block 120 and the By relatively rotating the lower block 130, it is possible to form a connection state with each other.

Through the lower hole 131, a sample for which a pretreatment process has been completed is transferred to the amplification chamber 132, and a cartridge that performs a final pretreatment process among cartridges provided in the intermediate block 120, for example, The fourth cartridge 340 and the amplification chamber 132 are connected.

That is, an opening 129 is formed on the lower surfaces of the fourth cartridge 340 and the fourth hole 340, but while the pretreatment process is performed through the fourth cartridge 340, the opening 129 is It is sealed by the upper surface of the lower block 130.

However, when the pretreatment process is finished, the intermediate block 120 rotates with respect to the lower block 130, or the lower block 130 rotates with respect to the intermediate block 120, so that the opening 129 ) Is aligned with the lower hole part 131, and accordingly, a sample for which the pretreatment process is completed in the fourth cartridge 340 may be provided to the amplification chamber 132 through the lower hole part 131.

The amplification chamber 132 receives heat from the heating unit 410 located under the amplification chamber 132 in a state in which the sample for which the pretreatment process has been completed is accommodated.

Accordingly, the sample is activated by heating of the heating unit 410, and through the detection unit 420 connected to the heating unit 410, the viral nucleic acid template of the sample is amplified and the amplified nucleic acid template is Confirmation, that is, detection.

Thus, the amplification of the nucleic acid template for the sample is completed, and the nucleic acid template thus completed is provided to the outside through the external chamber 500.

To this end, the amplification chamber 132 may be designed to allow insertion or detachment of the external chamber 500, and accordingly, the nucleic acid template stored in the amplification chamber 132 is externally transmitted through the external chamber 500. Flow into, and molecular diagnostics are performed.

Hereinafter, a step of performing a pretreatment process for the sample using the molecular diagnostic cartridge 10 having the above structure will be described in detail.

2A to 2E are schematic diagrams showing each step of the operation of the molecular diagnostic cartridge of FIG. 1. 3A to 3E are schematic diagrams specifically showing the operating state of the molecular diagnostic cartridge in FIGS. 2A and 2B.

First, referring to FIG. 3A, first, the upper space 111 of the upper block 110 is rotated to align with the first hole 121 of the intermediate block 120, that is, the first cartridge 130. Located.

In addition, the extraction unit 200 is drawn in through the upper space 111 in a state in which a sample has been extracted, as described above with the filter unit 230 inside, and is continuously drawn in a lower direction, and Fig. 3B And, as shown in FIG. 2A, the upper space 111 penetrates and is inserted into the first cartridge 130 to be positioned.

Thereafter, in a state located in FIGS. 3B and 2A, the handle part 220 of the extraction part 200 is operated to suck the solution stored in the first cartridge 130 into the filter part 230, Accordingly, the dissolution process for the sample is completed.

In this case, the suction process may be performed by operating the handle part 220 at least once or more, and may be repeated until sufficient dissolution for the sample is performed.

Thus, when the suction process is completed, as shown in FIG. 3C, the extraction unit 200 is moved to rise in a fixed state only in the upper space 111 of the upper block 110.

Thereafter, as shown in FIG. 3D, the upper block 110 rotates, for example, in a clockwise direction with respect to the intermediate block 120, and accordingly, the upper space 111 becomes the second hole ( 122), that is, positioned so as to be aligned with the second cartridge 320.

Thereafter, as shown in FIGS. 3E and 2B, the extraction unit 200 is inserted into the second cartridge 320 and positioned therein. And, in the state of being inserted into the second cartridge 320, when the handle 220 is similarly operated, the first cleaning liquid contained in the second cartridge 320 is sucked into the filter unit 230 and thus Accordingly, the first cleaning is performed on the sample.

In this case, the operation of the handle part 220 may be repeated, and accordingly, the first cleaning solution may be sufficiently sucked so that the first cleaning of the sample may be performed.

Thereafter, the upper block 110 and the extraction unit 200 repeat the same operation, and as shown in FIG. 2C, the extraction unit 200 is drawn into the interior of the third cartridge 330 Will be located. In addition, in the state of being inserted into the third cartridge 330, the second cleaning of the sample through the second cleaning solution included in the third cartridge 330 is similarly performed according to the operation of the handle unit 220. Performed.

Thereafter, in the same operation, an elution process for the sample as shown in FIG. 2D is performed.

In this case, the respective molecular diagnosis processes described with reference to FIGS. 2A to 2D are merely examples, and various processes may be selected and performed in consideration of characteristics of the sample.

As described above, when the pretreatment process for the sample is completed, as shown in FIG. 2E, the upper block 110 and the intermediate block 120 rotate with respect to the lower block 130, and the fourth The hole 124 and the lower hole part 131 are aligned. Accordingly, the opening 129 is also aligned with the lower hole 131.

Thereafter, the extraction part 200 is further entered in a downward direction, so that the end of the extension part 210 is on the opening 129 or through the opening 129 to form the lower hole part 131. It is arranged to be located on the entrance.

Thus, the sample on which the pretreatment process on the filter unit 230 has been completed is provided to the amplification chamber 132 through the lower hole part 131, and in the amplification chamber 132, the nucleic acid template is amplified as described above. .

4 is a schematic diagram showing a molecular diagnostic cartridge according to another embodiment of the present invention.

The molecular diagnosis cartridge 20 according to the present embodiment is generally formed in a square block shape, and accordingly, the molecular diagnosis described with reference to FIG. 1 except that the structure and shape of the block part, the structure and arrangement of the cartridge part, etc. are different. Since it is substantially the same as the cartridge 10, the same reference numerals are used for the same components, and duplicate descriptions are omitted.

Referring to FIG. 4, in the cartridge 20 in this embodiment, the block part 600 has a shape of a rectangular block extending in one direction as a whole, and an upper block 610, an intermediate block 620, and The lower block 630 is stacked.

The upper block 610 has an upper space 611 formed therein, and the extraction part 200 moves on the upper space 611.

The upper space 611 is formed in a longitudinal direction in the same direction as the extension direction of the upper block 610, and as shown, the upper block 610 is an outer wall sealing the outer surface of the upper space 611 It can be formed to contain only.

Accordingly, the upper space 611 includes an upper surface 612 and a lower surface 613 facing the upper surface 612, and the lower surface 613 may be substantially the upper surface of the intermediate block 620.

The intermediate block 620 is coupled to the lower portion of the upper block 610, and a plurality of holes 621, 622, 623, 624, 625 may be formed in a line at predetermined intervals, and each of the holes Cartridges 311, 321, 331, 341 may be inserted and fixed.

As described above, different process solutions may be stored in the first to fourth cartridges 311, 321, 331, and 341, respectively, and the type of stored solution and the number of cartridges vary according to embodiments. Can be changed.

In this embodiment, the hole 625 formed at the last position is a moving hole, and the cartridge is not inserted and the recessed hole state can be maintained. Thus, after the pretreatment process for the sample through the cartridges is all finished, it serves as a connection hole for providing the sample located in the extraction unit 200 to the amplification chamber 632 below.

The lower block 630 is coupled to the lower portion of the intermediate block 620, and the amplification chamber 632 and the amplification module 401 are disposed therein.

As described above, the amplification module 401 includes a heating unit 411 and a detection unit 421.

The amplification chamber 632 receives a sample for which a pretreatment process has been completed, and receives heat from a heating unit 411 located below while the sample is accommodated.

Accordingly, the sample is activated by heating of the heating unit 411, and through the detection unit 421 connected to the heating unit 411, the viral nucleic acid template of the sample is amplified and the amplified result is detected. This is done.

Thus, the amplification of the nucleic acid template for the sample is completed, and the nucleic acid template thus completed may be provided to the outside through an external chamber (not shown).

In this case, the structure and operation of the outer chamber are substantially the same as the outer chamber 500 described with reference to FIG. 1.

In a state in which the extraction part 200 is located in the upper space 611, it is repeatedly inserted and removed from each of the holes. In this embodiment, the extraction unit 200 is stably located in the upper space 611 when the insertion and removal of the extraction unit 200 is repeatedly operated, and the stroke of the extraction unit 200 is In order to keep it constant, a guide block 240 is provided in the extraction unit 200.

That is, the guide block 240 is fixed to the lower end of the extension part 210, and when the extraction part 200 is moved upward, additional movement is restricted by the upper surface 612, and the extraction When the part 200 is moved downward, additional movement is limited by the lower surface 612 to limit and maintain a constant movement stroke of the extension part 210 in the vertical direction.

Hereinafter, a step of performing a pretreatment process for the sample using the molecular diagnostic cartridge 20 having the above structure will be described in detail.

5A to 5E are schematic diagrams showing each step of the operation of the molecular diagnostic cartridge of FIG. 4.

First, referring to FIG. 5A, the extraction unit 200 is located inside the upper space 611 while a sample is handled by the filter unit 230.

Thereafter, referring to FIG. 5B, the extraction part 200 moves downward and is positioned so that the extension part 210 is inserted into the first hole 621, that is, the first cartridge 311. , The solution inserted into the first cartridge 311 is sucked into the filter unit 230 through the operation of the handle unit 220, thereby completing the dissolution process for the sample.

Thereafter, referring to FIG. 5C, the extraction part 200 moves upward, and the extension part 210 is separated from the first cartridge 311. Referring to FIG. 5D, the extraction part ( 200 is moved in one direction on the upper space 611.

Thereafter, although not shown through the drawings, the extracting unit 200 is aligned and positioned on the second hole 622, that is, the second cartridge 321, and then toward the inside of the second cartridge 321. It is inserted to perform a first cleaning process on the sample.

Thereafter, the extraction unit 200 is sequentially inserted into the third and fourth cartridges 331 and 341 to perform a second cleaning process and an elution process for the sample, respectively.

In this way, when the pretreatment process for the sample is completed, as shown in FIG. 5E, the extraction unit 200 is aligned on the moving hole 625 and then passes through the moving hole 625 to pass the It is located in the inner side of the lower hole (631).

Thus, in a state in which the end of the extension part 210 is drawn into the lower hole part 631, the sample, which has been pre-processed, is provided to the amplification chamber 632. Thereafter, in the amplification chamber 632, the nucleic acid template amplification process described above is performed, and if necessary, the amplified nucleic acid template may be provided to the outside.

As described above, through the molecular diagnostic cartridge 20 according to the present embodiment, a nucleic acid template can be amplified by performing a pretreatment process for a sample to be diagnosed, and in particular, the block part 600 is arranged in one direction. Accordingly, the user can more easily perform each process sequentially.

According to the embodiments of the present invention as described above, on the block portion divided into upper, middle and lower blocks, the extraction portion is inserted through the upper block, and cartridges for pretreatment of the sample are sequentially positioned in the middle block, The lower block is configured to receive and replicate a sample that has been finally pretreated, so that a molecular diagnostic cartridge can be configured with a relatively simple configuration, and the assembly and usability of the user are improved, and as a result, portability is improved. It may be readily available in diagnosis.

In particular, the extraction part is inserted or moved in the upper block, and the extraction part is composed of a fluid mixing part, and this mixing part is formed in various forms such as a dropper, syringe, and pressure/negative pressure pump, so that the user can easily handle it. It is configured to replicate a nucleic acid template through an amplification module, and all pre-processing for molecular diagnosis and all processes of nucleic acid analysis can be performed through one cartridge.

In addition, the block portion has a cylindrical shape, the upper block rotates with respect to the middle block, and the middle block rotates with respect to the lower block. Through this, process steps for each pre-treatment can be performed, so that the pre-treatment process is continued with a simple operation. Can be done.

In this case, the upper space on the upper block is located in a line according to the order of each process with the cartridges of the intermediate block, and each process can be easily performed through the extraction unit when aligned at the corresponding location, so that diagnosis speed and stability Diagnosis can be performed. In addition, it is possible to perform various pretreatment processes according to the type of sample by replacing the cartridge according to the required process.

On the other hand, on the cartridges arranged in a line in one direction, the extraction unit is sequentially moved and inserted so that each process can be performed continuously, so that only the rotational structure and the detailed operation method as described above are different. Diagnosis speed and stable diagnosis can be performed with simple operation.

However, in this case, by restricting the movement of the extraction unit in the vertical direction through the guide block, it is possible to prevent a problem due to the outflow of the sample.

Although the above has been described with reference to preferred embodiments of the present invention, those skilled in the art will be able to variously modify and change the present invention without departing from the spirit and scope of the present invention described in the following claims. You will understand that you can.

10, 20: molecular diagnostic cartridge
100, 600: block portion 110, 610: upper block
120, 620: middle block 130, 630: lower block
111, 611: upper space 131, 631: lower hole
200: extraction unit 230: filter unit
240: guide block 300, 301: cartridge part
400, 401: amplification module 410, 411: heating unit
420, 421: detection unit 500: outer chamber
612: upper surface 613: lower surface

Claims (13)

A block portion to which a plurality of cartridges are fixed, and an extraction portion connected to the cartridge to perform a pretreatment process for a sample,
The block part,
An intermediate block having a plurality of holes into which the cartridges are respectively inserted;
An upper block rotatably coupled to the intermediate block on an upper portion of the intermediate block and having an upper space aligned with one of the holes; And
A sample that is rotatably coupled to the intermediate block at a lower portion of the intermediate block and has a lower hole portion connecting between the amplification chamber and an end hole among the holes of the intermediate block, and the pretreatment is completed through the lower hole portion And a lower block provided to the amplification chamber,
The extraction unit is located outside the upper block when the upper block is rotated with respect to the intermediate block, and passes through any one of the upper space and the cartridge when the upper space is aligned with any one of the holes. Inhaling or discharging the solution in the cartridge,
After the pretreatment process is finished, when the lower block rotates with respect to the intermediate block so that the lower hole is aligned with the hole at the end, the extraction unit passes through the upper space, the hole at the end, and the lower hole. A molecular diagnostic cartridge, characterized in that providing the sample to the amplification chamber. The method of claim 1, wherein the cartridges,
A molecular diagnostic cartridge comprising a dissolution cartridge for dissolving the sample, a cleaning cartridge for cleaning the sample, and an elution cartridge for dissolving the sample. The method of claim 2,
Molecular diagnostic cartridge further comprising an amplification module comprising a heating unit for heating the pretreated sample of the amplification chamber under the amplification chamber, and a detection unit connected to the heating unit to duplicate and detect the nucleic acid template of the sample . The method of claim 3,
A molecular diagnostic cartridge that is attached to the amplification chamber and further comprises an external chamber for providing the cloned nucleic acid template to the outside. The method of claim 1, wherein the extraction unit,
An extension portion sequentially inserted into each of the cartridges;
A handle part for sucking and discharging the solution from the cartridge through the extension part; And
Molecular diagnostic cartridge, characterized in that it comprises a filter unit that is inserted into the extension and collects the sample and the solution. delete The method of claim 1, wherein the cartridges,
Molecular diagnostic cartridge, characterized in that inserted along the circumferential direction in the intermediate block. delete delete delete A block portion to which a plurality of cartridges are fixed, and an extraction portion connected to the cartridge to perform a pretreatment process for a sample,
The block part,
An intermediate block in which a plurality of holes into which the cartridges are respectively inserted are formed in a line along one direction;
An upper block coupled to an upper portion of the intermediate block and having an upper space moved along the one direction while the extraction part is inserted; And
A lower block coupled to the lower portion of the intermediate block and having a lower hole portion connecting between the amplification chamber and the end hole among the holes of the intermediate block, and receiving a pre-processed sample to the amplification chamber through the lower hole portion Including,
When the extraction unit is aligned with each of the holes, it is inserted into the holes to perform a pretreatment process for the sample, and when it is moved to an adjacent hole, it is separated from the holes and moved in the upper space,
The extraction unit includes a guide block stably positioning the extraction unit in the upper space and maintaining a constant stroke of the extraction unit when moving in the upper space,
The extraction unit, after the pre-treatment process is finished, after being aligned with the hole at the end, and passing through the hole at the end and the lower hole to provide the sample to the amplification chamber. The method of claim 11,
The guide block,
Characterized in that it is formed on an extension part that extends in the vertical direction from the extraction part and is sequentially inserted into each of the cartridges, and restricts the location of the extraction part by preventing the extraction part from being separated out of the upper space. Molecular diagnostic cartridge. delete

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