CN210030692U - Test tube structure combining PCR reaction tube and freeze-drying process - Google Patents
Test tube structure combining PCR reaction tube and freeze-drying process Download PDFInfo
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- CN210030692U CN210030692U CN201920511473.6U CN201920511473U CN210030692U CN 210030692 U CN210030692 U CN 210030692U CN 201920511473 U CN201920511473 U CN 201920511473U CN 210030692 U CN210030692 U CN 210030692U
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- 238000006243 chemical reaction Methods 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 31
- 238000004108 freeze drying Methods 0.000 title claims abstract description 29
- 238000012360 testing method Methods 0.000 title claims abstract description 28
- 238000012792 lyophilization process Methods 0.000 claims 2
- 239000012807 PCR reagent Substances 0.000 abstract description 21
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 14
- 238000003780 insertion Methods 0.000 abstract description 3
- 230000037431 insertion Effects 0.000 abstract description 3
- 239000011232 storage material Substances 0.000 abstract description 3
- 238000003752 polymerase chain reaction Methods 0.000 description 28
- 239000007788 liquid Substances 0.000 description 5
- 238000001514 detection method Methods 0.000 description 4
- 230000008901 benefit Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000003491 array Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 239000000443 aerosol Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 238000012631 diagnostic technique Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
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Abstract
The utility model discloses a test tube structure that combines PCR reaction tube and freeze-drying process, including pipe shaft and tube cap, the lateral wall of lower cylinder portion is equipped with the breach, can guarantee that at least partial breach opens when cylinder portion is gone up in the partial embedding of lower cylinder portion, guarantees when cylinder portion imbeds completely that the breach does not communicate with the external world. After the insertion depth of the tube cover exceeds a certain value, the tube cover can be stably kept on the tube body, the freeze-drying process can be carried out in the vacuum environment of the freeze dryer at the time, and then the tube cover is continuously covered in the vacuum environment of the freeze dryer after the freeze-drying process is finished. The technical scheme directly modifies the traditional PCR reaction tube, so that the PCR reaction tube can be used as a container for reagent storage and reagent reaction at the same time, and the use is convenient and efficient; because the PCR reagent is not contacted with the outside air, the freeze-dried PCR reagent has good quality and accurate test result. This utility model is used for the reagent field of preserving.
Description
Technical Field
The utility model relates to a reagent field of preserving especially relates to a test tube structure who combines PCR reaction tube and freeze-drying process.
Background
Polymerase Chain Reaction (Polymerase Chain Reaction, PCR): a molecular biological technique for amplifying specific DNA fragment can be regarded as special DNA duplication in vitro, and the biggest characteristic of PCR is that it can greatly increase trace amount of DNA.
Molecular diagnostic techniques, particularly the Polymerase Chain Reaction (PCR) technique, are used in large numbers in laboratories and clinical settings. The emergence of the fluorescence PCR technology solves the problem of laboratory pollution, and the PCR technology is more widely applied. The development trend of molecular detection is towards high-throughput, automation and instant detection, and the molecular detection is gradually expanded from the medical field to the application fields of animal quarantine, inspection and quarantine, food safety and the like.
The PCR reagent contains various bioactive materials. In the prior art, in order to maintain the activity of biological enzymes, PCR reagents are generally packaged in high concentration, and each component is stored in different centrifuge tubes and is stored and transported at the low temperature of-20 ℃. The PCR reagents were then ready for use. However, this brings many problems to the detection: 1. the operation is complicated, the consumed time is long, the liquid is not suitable to be kept in a high-temperature environment, the liquid can be polluted by aerosol in the air in the process of preparing the liquid (namely transferring the reagent and putting the reagent into a PCR reaction tube for dissolving), and the requirements on fields and personnel are high; 2. the low-temperature storage and transportation of the reagent are not beneficial to long-distance transportation, and simultaneously, the risk of overtemperature exists. The above factors limit the application and development of PCR technology in the basic layer.
PCR reagents in the test process, a plurality of control groups are usually arranged for testing, and accordingly, the quality requirement on the PCR reagents is very high. If a spot check reveals that a few PCR reagents in a batch are degenerated, more recent batches of PCR reagents are not recommended.
I researched and optimized the formula process of the PCR reagent, successfully processed the liquid PCR reagent into the powdery PCR reagent, and creatively solved the problem of difficult storage of the PCR reagent. Aiming at the processing process of converting liquid into powder, namely the freeze-drying process, I simultaneously put forward a solution of pre-dispensing and freeze-drying PCR reagents, the pre-dispensing and freeze-drying reagents require that a tube cover cannot be partially closed in the freeze-drying process so as to be convenient for pumping away water, and the tube cover is pressed tightly in the subsequent steps.
The existing PCR reaction tube can not realize the purpose due to the limitation of the existing consumables. Although the cover-opening freeze-drying mode can be adopted, the PCR reaction tube is taken out after freeze-drying and transferred to a clean environment, and the cover tube is covered, the mode can expose the PCR reagent in the air, and the PCR reagent can slowly absorb moisture in the atmosphere, so that the quality of the product is influenced.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a test tube structure who combines PCR reaction tube and freeze-drying process can guarantee PCR reagent quality, convenient to use moreover.
The utility model adopts the technical proposal that:
the utility model provides a combination PCR reaction tube and freeze-drying process's test tube structure, includes pipe shaft and tube cap, the pipe shaft includes last cylinder portion and lower circular cone portion that the fairing is connected, the tube cap includes roof and the lower cylinder portion that can insert last cylinder portion, and the degree of depth of going up cylinder portion is greater than the length of cylinder portion down, the lateral wall of cylinder portion is equipped with the breach down, and the diameter of going up cylinder portion and cylinder portion down is 6 ~ 12mm, the length of cylinder portion down is greater than 4mm, the top of breach is greater than 4mm with the lower extreme distance of cylinder portion down, can guarantee that at least partial breach is open when cylinder portion imbeds cylinder portion down, guarantees when cylinder portion imbeds cylinder portion completely down that the breach does not communicate with the external world.
As an improvement of the scheme, the side wall of the lower cylindrical part is provided with three annular bulges.
As an improvement of the scheme, when the lower cylindrical part is embedded into the upper cylindrical part and the gap part is opened, at least one annular bulge is lapped on the inner wall of the upper cylindrical part.
As an improvement of the scheme, the notch is arranged in the middle of the lower cylindrical part.
As an improvement of the scheme, the lower end of the notch extends to the lower end of the upper flush cylindrical part.
The utility model provides a combine PCR reaction tube and freeze-drying process's test tube structure, includes a plurality of above-mentioned test tube structure, a plurality of test tube structure arrays distribute into one row, are equipped with the connecting piece between two adjacent pipe shafts and between two adjacent tube caps.
As the improvement of the scheme, the pipe bodies and the connecting pieces thereof are of an integrated structure, and the pipe covers and the connecting pieces thereof are of an integrated structure.
The utility model has the advantages that: the tube cover in the test tube structure can be temporarily covered on the tube body, and researches show that for the small-sized tube body, after the insertion depth of the tube cover exceeds a certain value, the tube cover can be stably kept on the tube body, so that the freeze-drying process can be carried out in the vacuum environment of the freeze dryer, and then the tube cover is continuously covered in the vacuum environment of the freeze dryer after the freeze-drying process is finished. The technical scheme directly modifies the traditional PCR reaction tube, so that the PCR reaction tube can be used as a container for reagent storage and reagent reaction at the same time, and the use is convenient and efficient; because the PCR reagent is not contacted with the outside air, the freeze-dried PCR reagent has good quality and accurate test result.
Drawings
The present invention will be further explained with reference to the accompanying drawings:
FIG. 1 is a sectional view showing a structure of a test tube in a first embodiment when separated;
FIG. 2 is a sectional view showing the structure of the test tube closed in the first embodiment;
FIG. 3 is a sectional view showing the structure of the cuvette in a separated state in the second embodiment;
FIG. 4 is a sectional view showing the structure of the cuvette in a separated state in the third embodiment.
Detailed Description
Referring to fig. 1 to 4, the present invention relates to a test tube structure combining a PCR reaction tube and a freeze-drying process, which is based on a conventional PCR reaction tube and comprises a tube body 1 and a tube cover 2. The pipe body 1 and the pipe cover 2 are both of a rotary body structure, so that a half-section drawing is mainly used in the drawing of the specification.
The pipe body 1 comprises an upper cylindrical part 11 and a lower conical part 12 which are connected smoothly, and the top ends of the upper cylindrical part 11, the lower conical part 12 and the lower conical part 12 are made into round-angle structures. The tube cover 2 includes a top plate 21 and a lower cylindrical portion 22, and the top plate 21 seals an upper end of the lower cylindrical portion 22. Since the diameter of the lower conical portion 12 gradually shrinks, the depth of the upper cylindrical portion 11 needs to be larger than the length of the lower cylindrical portion 22, so as to ensure that the lower cylindrical portion 22 can be smoothly inserted into the upper cylindrical portion 11. The side wall of the lower cylindrical portion 22 is provided with a gap 24, the diameters of the upper cylindrical portion 11 and the lower cylindrical portion 22 are both 6-12 mm, the length of the lower cylindrical portion 22 is larger than 4mm, the distance between the uppermost end of the gap 24 and the lower end of the lower cylindrical portion 22 is larger than 4mm, when the lower cylindrical portion 22 is partially embedded into the upper cylindrical portion 11, at least part of the gap 24 is ensured to be open, and when the lower cylindrical portion 22 is completely embedded into the upper cylindrical portion 11, the gap 24 is ensured not to be communicated with the outside. It was found that for small-sized tube bodies 1, the tube cap 2 can be stably held on the tube body 1 after the insertion depth of the tube cap 2 exceeds a certain value, and at this time, the freeze-drying process can be performed in the vacuum environment of the freeze dryer, and then the tube cap 2 is continuously closed in the vacuum environment of the freeze dryer after the freeze-drying process is finished, and at this time, the gap 24 is ensured to be completely covered. For the traditional PCR reaction tube, when the tube cap 2 is put on, the tube cap 2 is askew and twisted, and the difficulty of covering the tube cap 2 by using a mechanical arm is very high.
The technical scheme directly modifies the traditional PCR reaction tube, so that the PCR reaction tube can be used as a container for reagent storage and reagent reaction, after a customer takes a pre-packaged reagent, a proper amount of sample is added, the mixture can be directly put into a PCR amplification instrument for test after uniform mixing, the tube does not need to be transferred and replaced, and the use is convenient and efficient; the efficiency of closing the pipe cover by using the mechanical arm is much higher than that of the traditional mode. Because the PCR reagent is not contacted with the outside air, the freeze-dried PCR reagent has good quality and accurate test result.
The overall length of a conventional PCR reaction tube is about 50mm, wherein the length of the upper cylindrical portion 11 can be extended as required, and the lower conical portion 12 is sized to ensure that the reagent occupies only the height of the lower conical portion 2/3.
Based on the above dimensions, the outer diameter of the lower cylindrical portion 22 is 7mm, and the inner diameter of the upper cylindrical portion 11 is also 7 mm. The length of the lower cylindrical portion 22 is 8mm, and the depth of the upper cylindrical portion 11 is 12 mm. The lower cylindrical portion 22 can be inserted 4mm into the upper cylindrical portion 11 in use and then can be stably placed. And in the subsequent covering step, the operation is finished through a manipulator positioned in the vacuum machine.
Preferably, three annular protrusions 23 are provided on the sidewall of the lower cylindrical portion 22, and the three annular protrusions 23 are respectively located at different heights. This design can improve the sealability between the lower cylindrical portion 22 and the upper cylindrical portion 11. Further, when the lower cylindrical portion 22 is fitted into the upper cylindrical portion 11 and the gap 24 is partially opened, at least one annular projection 23 overlaps the inner wall of the upper cylindrical portion 11. This can increase the stability of the tube cover 2. In this embodiment, two annular protruding positions in the top of breach, one annular protruding position in the below of breach, the annular protruding distance of the most lateral margin and below of breach is about 1 mm.
In the first embodiment, the notch 24 is disposed at the middle position of the lower cylindrical portion 22, and since the notch 24 only functions to allow water to flow out during the freeze-drying process, the size and position of the notch 24 do not affect the freeze-drying process. In yet another embodiment, the lower end of the notch 24 extends to be flush with the lower end of the upper cylindrical portion 11. The number of the notches 24 is generally one or two.
The utility model provides a combine PCR reaction tube and freeze-drying process's test tube structure, includes a plurality of above-mentioned test tube structure, a plurality of test tube structure arrays distribute into one row, are equipped with the connecting piece between two adjacent pipe shafts 1 and between two adjacent tube caps 2. The pipe bodies 1 and the connecting pieces thereof are of an integrated structure, and the pipe covers 2 and the connecting pieces thereof are of an integrated structure.
FIG. 4 shows a structure of a test tube having eight tubes. This scheme adapts to actual experimental environment, all can have a plurality of positions to place PCR reaction tube on the PCR instrument, produces test tube structure not only can practice thrift the cost one by one, and it is also very convenient to use moreover. Still an advantage, be exactly that a plurality of tube caps 2 all are connected with pipe shaft 1, and tube cap 2 has a plurality of strong points, and tube cap 2 can be placed more stably in freeze-drying process, also does benefit to follow-up manipulator lid more and closes.
Of course, the design creation is not limited to the above embodiments, and the combination of different features of the above embodiments can also achieve good effects. Those skilled in the art can make equivalent changes or substitutions without departing from the spirit of the present invention, and such equivalent changes or substitutions are included in the scope defined by the claims of the present application.
Claims (7)
1. A test tube structure combining a PCR reaction tube and a freeze-drying process is characterized in that: the pipe comprises a pipe body and a pipe cover, wherein the pipe body comprises an upper cylindrical part and a lower conical part which are connected in a fairing mode, the pipe cover comprises a top plate and a lower cylindrical part which can be inserted into the upper cylindrical part, the depth of the upper cylindrical part is larger than the length of the lower cylindrical part, a gap is formed in the side wall of the lower cylindrical part, the diameters of the upper cylindrical part and the lower cylindrical part are 6-12 mm, the length of the lower cylindrical part is larger than 4mm, the distance between the uppermost end of the gap and the lower end of the lower cylindrical part is larger than 4mm, at least part of the gap can be guaranteed to be opened when the lower cylindrical part is partially embedded into the upper cylindrical part, and the gap is guaranteed not to be communicated with the outside when the lower cylindrical part.
2. The cuvette structure combining a PCR reaction tube and a freeze-drying process according to claim 1, wherein: and three annular bulges are arranged on the side wall of the lower cylindrical part.
3. The cuvette structure combining a PCR reaction tube and a freeze-drying process according to claim 2, wherein: when the lower cylindrical portion is embedded into the upper cylindrical portion and the gap portion is open, at least one annular protrusion is lapped on the inner wall of the upper cylindrical portion.
4. The cuvette structure combining a PCR reaction tube and a lyophilization process according to any one of claims 1 to 3, wherein: the notch is arranged in the middle of the lower cylindrical part.
5. The cuvette structure combining a PCR reaction tube and a lyophilization process according to any one of claims 1 to 3, wherein: the lower extreme of breach extends to the lower extreme of cylinder portion on the parallel and level.
6. A test tube structure combining a PCR reaction tube and a freeze-drying process is characterized in that: a plurality of test tube structures according to any one of claims 1 to 5 are included, a plurality of test tube structures are arranged in a row, and connecting pieces are arranged between two adjacent tube bodies and between two adjacent tube covers.
7. The cuvette structure combining a PCR reaction tube and a freeze-drying process according to claim 6, wherein: the pipe bodies and the connecting pieces thereof are of an integrated structure, and the pipe covers and the connecting pieces thereof are of an integrated structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920511473.6U CN210030692U (en) | 2019-04-15 | 2019-04-15 | Test tube structure combining PCR reaction tube and freeze-drying process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920511473.6U CN210030692U (en) | 2019-04-15 | 2019-04-15 | Test tube structure combining PCR reaction tube and freeze-drying process |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210030692U true CN210030692U (en) | 2020-02-07 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920511473.6U Active CN210030692U (en) | 2019-04-15 | 2019-04-15 | Test tube structure combining PCR reaction tube and freeze-drying process |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210030692U (en) |
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2019
- 2019-04-15 CN CN201920511473.6U patent/CN210030692U/en active Active
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: A test tube structure combining PCR reaction tube and freeze drying process Effective date of registration: 20201127 Granted publication date: 20200207 Pledgee: Bank of China Limited by Share Ltd. Guangzhou Panyu branch Pledgor: GUANGDONG HUAYIN MEDICINE TECHNOLOGY Co.,Ltd. Registration number: Y2020440000374 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Granted publication date: 20200207 Pledgee: Bank of China Limited by Share Ltd. Guangzhou Panyu branch Pledgor: GUANGDONG HUAYIN MEDICINE TECHNOLOGY CO.,LTD. Registration number: Y2020440000374 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: A test tube structure combining PCR reaction tube and freeze-drying process Granted publication date: 20200207 Pledgee: Bank of China Limited by Share Ltd. Guangzhou Panyu branch Pledgor: GUANGDONG HUAYIN MEDICINE TECHNOLOGY CO.,LTD. Registration number: Y2024980026555 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right |