CN218146666U

CN218146666U - Exempt from PCR reaction tube of uncapping

Info

Publication number: CN218146666U
Application number: CN202222097927.4U
Authority: CN
Inventors: 吴祖飞; 赵百慧; 迟忠友
Original assignee: Shanghai Berger Medical Technology Co ltd
Current assignee: Shanghai Berger Medical Technology Co ltd
Priority date: 2022-08-10
Filing date: 2022-08-10
Publication date: 2022-12-27
Anticipated expiration: 2032-08-10

Abstract

The utility model discloses a relate to biological assay technical field, further relate to a PCR reaction tube of exempting from to uncap, include: the tube body is provided with a containing bin and can be used for containing reagents; the sealing element is sealed at the opening at the upper part of the tube body, a soft film structure is arranged corresponding to the opening of the tube body, and a cutting groove is formed in the soft film structure. After the tube body is preloaded with the reagent, the cutting groove is sealed, the sealing piece and the tube body form a closed structure, when sampling or sample adding is needed, the cutting groove is opened by external force, the flexible membrane structure is broken, and an opening for the sampler to enter is formed in the sealing piece. The PCR reaction tube capable of avoiding uncovering when a sample is added is realized, and the problems that reagents are spilled and secondary pollution are caused easily when the existing PCR reaction tube is uncovered are solved.

Description

Exempt from PCR reaction tube of uncapping

Technical Field

The utility model relates to a biological assay technical field has further related to a PCR reaction tube of exempting from to uncap.

Background

The PCR technology is a common molecular diagnosis technology, has the advantages of high sensitivity, strong specificity, short diagnosis window period and the like, can carry out qualitative and quantitative detection, can be widely used for diagnosing diseases such as hepatitis, venereal disease, lung infectious diseases, prenatal and postnatal care, genetic disease genes, tumors and the like, fills the detection blank of the early immunodetection window period, and provides effective help for early diagnosis, early treatment and safe blood consumption.

However, in the process of using the PCR technology, the preparation of the reagent is complex, and the requirement on the professional level of the operator is high, so the complicated process of reagent reconfiguration is avoided by adopting a method of pre-loading the reagent or a method of pre-lyophilizing the reagent in the PCR tube. In the prior art, after a reagent is preloaded, sealing is generally performed by adopting aluminum film heating, and generally the aluminum film heating can be performed to 180 ℃, so that the preloaded reagent or lyophilized powder can be invalid. And when sample and application of sample, often need uncap the application of sample, because of the leakproofness consideration, the tube cap is generally comparatively inseparable with the combination of body, so often can be because the action range of uncapping causes reagent to spill or the freeze-dried powder spill greatly in this process, and causes secondary pollution easily.

SUMMERY OF THE UTILITY MODEL

The utility model provides a exempt from PCR reaction tube of uncapping, through correspond on PCR reaction tube's the sealing member body open position sets up the mantle structure, and set up on the mantle structure and supply sample head male grooving, realized one kind and avoided uncapping when adding the sample and avoid the PCR reaction tube of heat seal, solved current PCR reaction tube and caused the problem that reagent spills easily when uncapping, and sealed the problem that can cause reagent to be heated inefficacy of aluminium membrane when pre-installation reagent.

Specifically, the utility model provides a exempt from PCR reaction tube of uncapping, include:

the tube body is provided with a containing bin and can be used for containing reagents;

the sealing element is sealed at the opening at the upper part of the pipe body, a soft film structure is arranged corresponding to the opening of the pipe body, and a cutting groove is formed in the soft film structure;

after the tube body is preloaded with the reagent, the cutting groove is sealed, the sealing piece and the tube body form a closed structure, when sampling or sample adding is needed, the cutting groove is opened by external force, the soft membrane structure is broken, and an opening for the sampler to enter is formed in the sealing piece.

In some embodiments, the sealing part of the sealing element is annularly provided with a sealing protrusion, and the inner wall of the pipe body is provided with a limiting groove matched with the sealing protrusion. After the sealing bulge is embedded into the pipe body, the sealing bulge can be limited to move, and the sealing performance is improved.

In some embodiments, the sealing device further comprises a tube cover capable of covering the sealing element, and the tube cover can perform secondary sealing on the tube body after the sealing element cutting groove is opened.

In some embodiments, the tube cover has a downward protrusion, the protrusion can be engaged with the opening of the sealing member, and the protrusion has a finger insertion slot on the outer side of the tube cover for facilitating the finger insertion to open the tube cover.

In some embodiments, the slit of the sealing member is radial, and the sampling head only needs to apply a force to the center of the slit, and the flexible membrane structure is broken to facilitate the insertion and sampling operation of the sampling head.

In some embodiments, the sealing member is made of a silica gel material, the silica gel material is non-toxic and harmless, and does not affect reagents in the PCR reaction tube, and the silica gel material has good sealing performance and does not generate air leakage and the like. In addition, the silica gel has stable property, generally does not react with other chemical substances, and has wide application scenes.

In some embodiments, the pipe body fixing device further comprises a clamping plate, wherein a plurality of clamping positions for the pipe body to be inserted are arranged on the clamping plate, and the clamping plate can be used for fixing a plurality of groups of pipe bodies so as to facilitate the movement of the pipe bodies.

In some embodiments, the seal includes a seal body and a plurality of sealing flanges integrally formed with the seal body, the sealing flanges corresponding to the detents and being insertable into the tube to seal against the spout. The sealing flanges and the sealing element main body are integrally formed, the sealing element is integrally formed into a sealing plug, the sealing flanges can be embedded into the tube body in a downward pressing mode to seal the opening of the tube body, and the operation is simple and convenient.

In some embodiments, the tubes are arranged on the clamping plate in 4-tube rows, 8-tube rows or 96-tube rows, so that PCR detection can be performed on a large scale at one time, and the detection efficiency is improved.

In some embodiments, the tube body can be made of high-light-transmittance plastic, so that subsequent operations such as fluorescence detection can be performed conveniently without replacing the reaction tube.

Compared with the prior art, the utility model discloses a following beneficial effect has at least:

1. the pipe body is provided with a sealing element which can seal the pipe body, the position, corresponding to the pipe opening, of the sealing element is of a soft film structure, and a cutting groove is formed in the soft film structure. After the tube body is preloaded with the reagent, the cutting groove is in a sealing state, the sealing element and the tube body form a sealing structure, when sampling or sample adding is needed, the cutting groove is opened by external force, the flexible membrane structure is broken, and an opening for the sampler to enter is formed in the sealing element. This kind of reaction tube need not to uncap when adding the sample again, because the action range causes reagent to spill easily when avoiding uncapping the application of sample greatly, perhaps takes place secondary pollution's the condition.

2. The tube body is sealed by a sealing element, and the sealing element is usually sealed by a sealing plug. Under the condition of keeping air tightness and good sealing performance, the tube body is sealed in other modes, and the condition that the reagent in the tube is heated and fails in the traditional sealing mode is avoided.

3. The application PCR reaction pipe can also include the tube cap, after adding the sample article, can be through closing the tube cap lid on the sealing member, carry out secondary seal to the body, make PCR reaction pipe can the secondary use, can not take place cross contamination when just adding the sample article.

4. This application simple structure, preparation process flow is simple, when the mode that adopts the plug seals, only need can accomplish through the mode that adds the stopper and press behind the pre-installation reagent and seal, need not to seal through the mode of aluminium membrane heating, and required equipment is simple, saves the cost of reagent pre-installation.

Drawings

The above features, technical features, advantages and modes of realisation of the present invention will be further described in the following detailed description of preferred embodiments thereof, which is to be read in conjunction with the accompanying drawings.

FIG. 1 is an exploded view of an embodiment of the present invention;

FIG. 2 is a schematic view of the structure of the tube body and the clip plate of the present invention;

FIG. 3 is a schematic longitudinal sectional view of FIG. 1;

fig. 4 is a top view of the sealing member structure of the present invention;

fig. 5 is a front view of the sealing member of the present invention;

FIG. 6 is a schematic diagram of the longitudinal structure after the secondary sealing;

fig. 7 is a schematic view of the structure of the tube cap of the present invention.

The reference numbers illustrate:

a tube body 1, a tube body opening 11, an accommodating bin 12, a limiting groove 13,

the sealing member 2, the soft membrane structure 21, the undercut groove 211, the annular flange 22, the sealing projection 221, the sealing member body 23,

the card 3 is provided with a plurality of card holders,

and a tube cover 4.

Detailed Description

In order to more clearly illustrate embodiments of the present invention or technical solutions in the prior art, specific embodiments of the present invention will be described below with reference to the accompanying drawings. It is obvious that the drawings in the following description are only examples of the invention, and that for a person skilled in the art, other drawings and embodiments can be obtained from these drawings without inventive effort.

For the sake of simplicity, only the parts related to the present invention are schematically shown in the drawings, and they do not represent the actual structure as a product. Moreover, in the interest of brevity and understanding, only one of the components having the same structure or function is illustrated schematically or designated in some of the drawings. In this document, "a" means not only "only one of this but also a case of" more than one ".

It should be further understood that the term "and/or" as used in this specification and the appended claims refers to and includes any and all possible combinations of one or more of the associated listed items.

In this context, it is to be understood that, unless otherwise explicitly stated or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood as a specific case by those skilled in the art.

In addition, in the description of the present application, the terms "first", "second", and the like are used only for distinguishing the description, and are not intended to indicate or imply relative importance.

In one embodiment, referring to fig. 1, fig. 2, fig. 3, and fig. 4 of the specification, the present invention provides a cap-opening-free PCR reaction tube, which comprises a tube body 1 and a sealing member 2. The tube body 1 is provided with a containing bin 12 which can be used for containing reagents; the sealing element 2 is sealed at the upper opening of the tube body 1, a soft film structure 21 is arranged at the position corresponding to the tube body opening 11, and a cutting groove 211 is formed in the soft film structure 21. After the tube body 1 is preloaded with the reagent, the cutting groove 211 is in a sealing state, and the sealing element 2 and the tube body 1 form a sealing structure, so that the condition that the preloaded reagent or the freeze-dried reagent is deteriorated or loses efficacy due to air leakage is prevented; when a sample is needed or a sample is added, the cutting groove 211 is opened by external force, the flexible membrane structure 21 is broken, an opening for the sampler to enter is formed on the sealing element 2, the sampler enters the containing bin 12 of the tube body 1 through the opening, and the sampling or sample adding work is completed.

In a preferred embodiment, referring to the description of fig. 1, 5 and 6, the sealing part of the sealing element 2 is provided with a sealing protrusion 221, and the inner wall of the tube body 1 is provided with a limiting groove 13 matched with the sealing protrusion 221. After the sealing protrusion 221 is embedded into the tube body 1, the limiting groove 13 can limit the movement of the sealing protrusion 221, so that the sealing effect between the sealing ring and the tube body 1 is improved.

In a preferred embodiment, referring to fig. 6 and 7 of the specification, the uncapping-free PCR reaction tube further comprises a tube cap 4 that can be closed on the sealing member 2, and the tube cap 4 can perform secondary sealing on the tube body 1 after the cutting groove 211 of the sealing member 2 is opened. The tube cover 4 can cover the opening formed on the sealing element 2 for the sampler to enter after the soft film structure 21 is broken, and secondary sealing is carried out after the sample is added.

In a preferred embodiment, referring to fig. 7 of the specification, the cap 4 has a downward projection which is fitted into the opening of the sealing member 2, and the projection is provided with a finger insertion groove on the outer side of the cap 4 for inserting a finger. An operator can insert one finger into the groove, and the other finger grasps the edge of the tube cover 4 to open the tube cover 4, so that labor is saved during operation.

In a preferred embodiment, referring to the description attached to fig. 1 and fig. 2, the uncapping-free PCR reaction tube further comprises a clamping plate 3, wherein the clamping plate 3 is provided with a plurality of clamping positions, the tube body 1 can be fixed on the clamping plate 3, and the plurality of clamping positions can combine a plurality of PCR reaction tubes together, so that the PCR detection can be conveniently carried out in batches. Specifically, the tubes may be arranged on the pallet in 4, 8, or 96-tube rows.

Correspondingly, the sealing element 2 comprises a sealing element body 23 and a plurality of sealing flanges which are integrally formed with the sealing element body 23, correspond to the above-mentioned detents and can be inserted into the tube body 1 to seal the tube mouth. The sealing flanges and the sealing member main body 23 are integrally formed, at the moment, the sealing member 2 is integrally formed into a sealing plug, the sealing flanges can be embedded into the pipe body 1 in a downward pressing mode to seal the pipe body opening 11, the operation is simple, and the production operation is convenient.

In one embodiment, referring to the attached figure 1 of the specification, the PCR reaction tube comprises a tube body 1, a sealing member 2, a clamping plate 3 and a tube cover 4, wherein the tube body 1 can be used for pre-loading reagents and containers for subsequently adding samples and extracting samples; the sealing element 2 is integrally in the form of a sealing plug, a sealing flange is integrally formed on the sealing element, the sealing flange is in a concave structure when viewed from the top, the bottom is a soft membrane structure 21, and a cutting groove 211 is formed in the soft membrane structure 21 and can be penetrated by a sampling head; the part of the sealing flange embedded with the pipe body 1 is annularly provided with a sealing bulge 221, and a corresponding position of the pipe body 1 is provided with a limit groove 13; the tube body 1 can be inserted into a blocking position on the clamping plate 3 to form a multi-tube row form.

After the tube body 1 is pre-filled with the reagent, the sealing flange on the sealing element 2 is embedded into the opening 11 of the tube body, so that a sealing effect is achieved. When needs application of sample or sample, the tip of sampler exerts external force to mantle structure 21, because mantle structure 21 department is equipped with grooving 211, mantle structure 21 breaks more easily, is convenient for add the sample. After the sample is added, the pipe cover 4 can be covered at the opening of the sealing element 2, so that the secondary sealing effect is achieved.

The sealing member 2 may be a soft rubber material, such as silica gel, rubber, and the like, and is preferably silica gel. The silica gel material is non-toxic and harmless, does not influence reagents in the PCR reaction tube, has good sealing performance, and does not generate the phenomena of air leakage and the like. In addition, the silica gel has stable property, generally does not react with other chemical substances, and has wide application scenes.

In a preferred embodiment, with reference to fig. 4 of the accompanying description, the slots 211 of the sealing element 2 are shaped radially, in particular in the form of a cross, or three or five radial lines.

In a preferred embodiment, the tube body 1 can be made of a high light-transmitting plastic, preferably polypropylene, so as to facilitate subsequent fluorescence detection and the like without replacing the reaction tube.

To sum up, this application provides a exempt from PCR reaction tube of uncapping seals body 1 through the form that adopts sealing member 2 to set up the mantle structure 21 in corresponding body opening 11 position, and set up grooving 211 on corresponding mantle structure 21, reach one kind and need not to carry out the PCR tube of uncapping when adding the sample, avoid causing reagent to spill or secondary pollution's the condition when uncapping.

It should be noted that the above embodiments can be freely combined as necessary. The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of improvements and decorations can be made without departing from the principle of the present invention, and these improvements and decorations should also be regarded as the protection scope of the present invention.

Claims

1. A PCR reaction tube without opening a cover is characterized by comprising:

after the tube body is preloaded with the reagent, the cutting groove is sealed, the sealing piece and the tube body form a closed structure, when sampling or sample adding is needed, the cutting groove is opened by external force, the flexible membrane structure is broken, and an opening for a sampler to enter is formed in the sealing piece.

2. The uncapping-free PCR reaction tube according to claim 1, wherein the sealing part of the sealing element is annularly provided with a sealing protrusion, and the inner wall of the tube body is provided with a limiting groove matched with the sealing protrusion.

3. The cap-free PCR reaction tube of claim 1, further comprising a cap covering the sealing member, wherein the cap can perform a secondary sealing on the tube body after the sealing member cutting groove is opened.

4. The cap-free PCR reaction tube of claim 3, wherein the tube cap has a downward protrusion which is engaged with the opening of the sealing member, and the protrusion has a finger insertion groove on the outer side of the tube cap.

5. The uncapped PCR reaction tube according to claim 1, wherein the shape of the cutting groove of the sealing member is radial.

6. The cap-free PCR reaction tube of claim 1, wherein the sealing member is made of silicone material.

7. The uncapped PCR reaction tube according to any one of claims 1 to 6,

still include the cardboard, be equipped with a plurality of screens that can supply the body is inserted and is established on the cardboard.

8. The cap-free PCR reaction tube of claim 7, wherein the sealing member comprises a sealing member main body and a plurality of sealing flanges integrally formed with the sealing member main body, the sealing flanges corresponding to the locking positions and being inserted into the tube body to seal the tube mouth of the PCR reaction tube.

9. The uncapping-free PCR reaction tube according to claim 7, wherein the tubes are arranged in 4-tube rows, 8-tube rows, or 96-tube rows on the chuck plate.

10. The uncapping-free PCR reaction tube according to claim 1, wherein the tube body is made of high-light-transmittance plastic.