CN109580973A - Detection kit and biochemical immunity detection method - Google Patents

Abstract

The present invention is suitable for in-vitro diagnosis (IVD) technical field, disclose detection kit and biochemical immunity detection method, wherein, detection kit includes for accommodating the first box body of the first reagent, the second box body for accommodating the second reagent, for covering to close is installed on box cover at the top of the first box body, and the first box body includes that bottom can be inserted into the hollow housing that in the second box body and top can be stuck in outside the second box body, the sealing-plug for sealing hollow housing bottom, for sealing the diaphragm seal at the top of hollow housing.Biochemical immunity detection is carried out using detection kit of the invention, a liquid relief sample-adding is only needed, reduces the cumulative errors being repeatedly loaded, improve the accurate reliability of detection, while also improving the efficiency of detection and the fault risk of handwork；And detection kit constitutes the detection environment of a sealing, reduces the biological infection harm to operator, avoids to generate in the collection of waste, treatment process and endanger the biological infection of operator.

Description

Detection kit and biochemical immunity detection method

Technical field

The present invention relates to in-vitro diagnosis (IVD) technical field more particularly to detection kit and use the detection kit The biochemical immunity detection method detected.

Background technique

In-vitro diagnosis (IVD), in terms of being based particularly on the in-vitro diagnosis field of quick diagnosis (POCT), needed in detection by Human body or the body fluid of animal, wherein mainly blood (blood plasma, serum, whole blood etc.) is added in one or more kinds of reagents and carries out Biochemical reaction.The prior art, biochemical immunity detection substantially exist following two scheme:

1) multiple separate chambers are separated on a kit, then using liquid-transfering gun or machine successively it is separately sampled into Row carries out biochemical reaction after mixing.Biochemical immunity detection is carried out using the kit, the reagent for needing multiple liquid relief different is to together In one experiment sample reaction vessels, frequent liquid relief is needed in the detection process in this way.It tests, needs if it is using manual method Repeatedly sampling, time-consuming low efficiency, while having the pollution risk for generating biochemical harm, influence test result, or even harm test behaviour The health of author；And if tested using machine, need to overcome the cross contamination of each reagent, while to overcome and repeatedly sample Influence of the cumulative errors to test result.

2) reagent of solid phase or liquid phase is fixed in reaction box by encapsulation, then is successively separately added into sample and other Reagent.The program in the detection process, although reducing primary sample-adding, still needs repeatedly sample-adding and reagent, simultaneously Reaction box is unable to satisfy the requirement of mixing as reaction vessels in the program, also do not reduce to greatest extent be repeatedly loaded it is accumulative Error source, and the reaction box of open type is to the risk of the manual method of testing person that still remains Infection Action.

Summary of the invention

The first purpose of this invention is to provide a kind of detection kit, aims to solve the problem that existing biochemical immunity detected Detection kit used in journey needs repeatedly sample-adding, and that there are detection efficiencies is low, detection accurate reliability is low and easy to operator The technical issues of generating biological infection.

In order to achieve the above objectives, scheme provided by the invention is: detection kit, including for accommodating the first reagent One box body, the second box body for accommodating the second reagent are installed on box cover at the top of first box body for covering to close, and described the One box body includes that bottom can be inserted into the hollow housing that in second box body and top can be stuck in outside second box body, for close Seal the sealing-plug of the hollow housing bottom, for sealing the diaphragm seal at the top of the hollow housing.

Optionally, the hollow housing includes card insertion in second box body and on the inside of lateral wall and second box body First canister portion of wall sealing cooperation and the detent portion for being convexly equipped in first canister portion top and being stuck in outside second box body.

Optionally, the lateral wall of first canister portion is convexly equipped with several and is arranged along its axially spaced-apart for described the First cyclic annular convex ribs of two box body inner sidewalls sealing cooperation；And/or

The hollow housing further includes below first canister portion and outer diameter is less than the of the first canister portion outer diameter Two canister portions and the taper canister portion being connected between first canister portion and second canister portion；And/or

Annular recess is recessed at the top of the detent portion.

Optionally, the bottom of the box cover is convexly equipped with for across the diaphragm seal and the hollow housing and will be described close Blocking is opened so that the body of rod of first box body Yu second box body is connected.

Optionally, the box cover includes the body of rod, the card insertion body for cooperating with the hollow housing card insertion, is convexly equipped in Card insertion body top and the cover board being stuck in outside the hollow housing and be set to the cover board outer edge and for it is described hollow The back-off that the outside portion of shell is clasped, the body of rod are convexly equipped in the bottom end of the card insertion body.

Optionally, the cover board includes flat part and inclined plate portion, and the flat part deviates from from the top of card insertion body court The straight extension in direction of the card insertion body central axis, edge court far from the card insertion body of the inclined plate portion from the flat part Direction away from the card insertion body central axis extends obliquely downward, described to be buckled to from the separate flat part in the inclined plate portion Edge extends downwardly projection.

Optionally, the sealing-plug include for from hollow housing bottom card insertion in the intracorporal cock body of the hollow shell With the cock cap for being convexly equipped in described cock body one end and being stuck in outside the hollow housing, the cock body in an interference fit with it is described The cooperation of hollow housing card insertion.

Optionally, the lateral wall of the cock body is convexly equipped with several and is arranged along its axially spaced-apart to be used for and the hollow shell Second cyclic annular convex ribs of the inner sidewall sealing cooperation of body.

Optionally, the diaphragm seal is metal foil paper or metal foil；And/or

The sealing-plug is elastic plug；And/or

The bottom end of the box cover is detachably equipped with the sample capillary for taking blood sample.

Second object of the present invention is to provide a kind of biochemical immunity detection method comprising:

The first reagent is loaded in the first box body and seals the first box body, while the second reagent is loaded in the second box body, Then the first box body card insertion is installed on second box body；

By liquid transfer gun head, perhaps then sample capillary takes blood sample with the liquid transfer gun head or the sampling capillary Pipe punctures the diaphragm seal on first box body, and makes the blood sample and described first in the liquid transfer gun head or sample capillary First reagent mixing in box body；

Box cover is installed on first box body at the top of first box body, and presses the box cover down with logical It crosses the box cover and opens the sealing-plug, so that the blood sample, first reagent in first box body are flowed into institute It states in the second box body and is mixed with the second reagent；

It is artificial or the detection kit is shaken by mechanical mechanism, with shake uniformly the blood sample, the first reagent and Second reagent；

After the detection kit is stood a period of time, pass through detecting instrument interpretation testing result.

Detection kit provided by the invention and biochemical immunity detection method, by the way that two mutually independent first boxes are arranged Body and the second box body are to be designed as bottom pluggable second for accommodating the first reagent and the second reagent respectively, and by the first box body Box body is interior, top can be stuck in the structure that the second box body is outer and bottom is sealed by sealing-plug, top is sealed by diaphragm seal, in this way When detecting, by liquid transfer gun head, perhaps sample capillary takes blood sample then to be punctured with liquid transfer gun head or sample capillary Diaphragm seal on first box body, and keep the first reagent in the blood sample and the first box body in liquid transfer gun head or sample capillary mixed It closes, sealing-plug is then opened by box cover or other external mechanisms again, to realize blood sample, the first reagent and the second reagent Mixing, be uniformly mixed stand a period of time after can interpretation testing result.Compared with the existing technology, the solution of the present invention has Below the utility model has the advantages that

1) biochemical immunity detection is carried out using detection kit of the invention, only needs a liquid relief sample-adding, reduces repeatedly The cumulative errors of sample-adding reduce the fault risk of handwork, improve the accurate reliability and efficiency of detection；

2) after only need to being once loaded in detection, and subsequent action is easy to operate, operates convenient for a large amount of manual inspections, can also be with Realize automation continuous productive process, detection efficiency is high, and it is simple to operate, it is particularly suitable for quickly detecting in (POCT) project；

3) detection kit constitutes the detection environment of a sealing, has prevented the biological infection harm to operator；

4) after the completion of detecting, blood sample and reagent work as offal treatment in the form of sealed storage is in detection kit, reduce The collection of subsequent bio Biohazard Waste, processing difficulty, and avoid and generate in the collection of waste, treatment process to behaviour The biological infection of author endangers.

Detailed description of the invention

In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with The structure shown according to these attached drawings obtains other attached drawings.

Fig. 1 is the half-sectional decomposition diagram of detection kit provided in an embodiment of the present invention；

Fig. 2 is that the first box body provided in an embodiment of the present invention for being mounted with the first reagent is laminated on and is mounted with the second reagent Half-sectional assembling schematic diagram on second box body；

Fig. 3 is status diagram when loading onto box cover in Fig. 2；

Fig. 4 is by the box cover in Fig. 3 down by status diagram when opening sealing-plug；

Fig. 5 is the half-section diagram of the first box body provided in an embodiment of the present invention for being mounted with the first reagent；

Fig. 6 is the half-section diagram of the second box body provided in an embodiment of the present invention for being mounted with the second reagent；

Fig. 7 is the half-section diagram of box cover provided in an embodiment of the present invention；

Fig. 8 is the half-sectional assembling schematic diagram of box cover provided in an embodiment of the present invention and blood sampling capillary.

Specific embodiment

Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete Site preparation description, it is clear that described embodiment is only a part of the embodiments of the present invention, instead of all the embodiments.Base Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts it is all its His embodiment, shall fall within the protection scope of the present invention.

It is to be appreciated that the directional instruction (such as up, down, left, right, before and after ...) of institute is only used in the embodiment of the present invention In explaining in relative positional relationship, the motion conditions etc. under a certain particular pose (as shown in the picture) between each component, if should When particular pose changes, then directionality instruction also correspondingly changes correspondingly.

It should also be noted that, when an element is referred to as being " fixed " or " disposed " on another element, it can be straight Connect on the other element or may be simultaneously present centering elements.When an element is known as " connection " another element, It, which can be, is directly connected to another element or may be simultaneously present centering elements.

In addition, the description for being related to " first ", " second " etc. in the present invention is used for description purposes only, and should not be understood as referring to Show or imply its relative importance or implicitly indicates the quantity of indicated technical characteristic." first ", " are defined as a result, Two " feature can explicitly or implicitly include at least one of the features.In addition, the technical solution between each embodiment can It to be combined with each other, but must be based on can be realized by those of ordinary skill in the art, when the combination of technical solution occurs Conflicting or cannot achieve when, will be understood that the combination of this technical solution is not present, also not the present invention claims protection model Within enclosing.

As shown in figures 1-8, detection kit provided in an embodiment of the present invention, including for accommodating the first reagent 500 One box body 100, the second box body 200 for accommodating the second reagent 600 close the box for being installed on 100 top of the first box body for covering Lid 300, the first box body 100 includes in pluggable second box body 200 in bottom and top can be stuck in the hollow shell outside the second box body 200 Body 110, the sealing-plug 120 for sealing 110 bottom of hollow housing, the diaphragm seal 130 for sealing 110 top of hollow housing. The embodiment of the present invention is by two mutually independent first box bodys 100 of setting and the second box body 200 to be used to accommodate first respectively Reagent 500 and the second reagent 600, and the first box body 100 can card insertion laminate on the second box body 200, while by the first box body 100 It is designed as bottom and the structure that diaphragm seal 130 seals is passed through by the sealing of sealing-plug 120, top, the second box body 200 is designed as bottom Portion sealing, open top structure, in this way using detection kit provided in an embodiment of the present invention carry out biochemical immunity detection when, By liquid transfer gun head, perhaps sample capillary 400 takes blood sample then to puncture first with liquid transfer gun head or sample capillary 400 Diaphragm seal 130 on box body 100, and make in liquid transfer gun head or blood sample and the first box body 100 in sample capillary 400 One reagent 500 mixing, sealing-plug 120 is then opened by box cover 300 or other external mechanisms again, thus realize blood sample, The mixing of first reagent 500 and the second reagent 600, being uniformly mixed can interpretation testing result after standing a period of time.Using this The detection kit that inventive embodiments provide carries out biochemical immunity detection, only need liquid relief sample-adding, reduces and is repeatedly loaded Cumulative errors reduce the fault risk of handwork, improve the accurate reliability and efficiency of detection；And subsequent action operates Simply, it being operated convenient for a large amount of manual inspections, automation continuous productive process also may be implemented, detection efficiency is high, and it is simple to operate, especially It is suitable for quickly detecting in (POCT) project.In addition, detection kit constitutes the detection ring of a sealing in detection process Border has prevented the biological infection harm to operator；After the completion of detection, blood sample and reagent are with sealed storage in detection kit Form works as offal treatment, reduces collection, the processing difficulty of subsequent bio Biohazard Waste, and avoids the receipts in waste It is generated in collection, treatment process and the biological infection of operator is endangered.

Preferably, diaphragm seal 130 is bonded the top for being packaged in hollow housing 110.

Preferably, diaphragm seal 130 is the sheet body for being easy to be punctured by liquid transfer gun head or sample capillary 400, in this way, being convenient for Diaphragm seal 130 is quickly punctured by liquid transfer gun head or sample capillary 400 in detection process, to be conducive to blood sample injection the In one box body 100, detection efficiency is effectively increased.

Preferably, in the present embodiment, diaphragm seal 130 is metal foil paper or metal foil.Metal foil is prolonged with metal The foil transformed into, metal foil paper are a kind of extrusion coating papers being made of two kinds of materials of metal foil and body paper.Metal foil paper or Person's metal foil can meet the intensity of diaphragm seal 130, sealing performance requirement, and all be easy to by liquid transfer gun head or sampling capillary Pipe 400 punctures, and is packaged in addition, metal foil paper or metal foil all can be quickly bonded, and may make diaphragm seal 130 and the first box The assembly manipulation of body 100 is relatively easy realization.The foil paper of metal foil paper or metal foil concretely metals such as aluminium or tin Or paillon, as a preferred embodiment of the present embodiment, diaphragm seal 130 is aluminium-foil paper or aluminium foil.

Preferably, referring to shown in Fig. 2 and Fig. 5, hollow housing 110 include card insertion in the second box body 200 and lateral wall with The first canister portion 111 and be convexly equipped in 111 top of the first canister portion and be stuck in the second box body that the sealing of second box body, 200 inner sidewall cooperates Detent portion 112 outside 200.The clamping matching of detent portion 112 and the second box body 200 is, it can be achieved that be installed on to the first box body 100 Axial limiting on two box bodys 200.First canister portion 111 and the cooperation of the card insertion of the second box body 200 are, it can be achieved that the first box body 100 The radial limit being installed on the second box body 200, the lateral wall of the first canister portion 111 and the inner sidewall sealing of the second box body 200 are matched It closes, the reagent in detection process in detection kit, blood sample is thus prevented to match from the first canister portion 111 and the second box body 200 It closes outside gap outflow detection kit, effectively prevents the reagent in detection process, blood sample endangers the biological infection of operator Phenomenon occurs.

Preferably, referring to shown in Fig. 2 and Fig. 5, the lateral wall of the first canister portion 111 is convexly equipped with several and sets along its axially spaced-apart It sets for the first cyclic annular convex ribs 113 with the sealing cooperation of 200 inner sidewall of the second box body.In the present embodiment, the first canister portion 111 is logical It crosses the first cyclic annular convex ribs 113 and realizes and cooperate with the tight fit of the second box body 200 and sealing, be both conducive to reduce outside the first box body 100 The contact area of 200 inner sidewall of side wall and the second box body, to be conducive to reduce the assembly of the first box body 100 and the second box body 200 Difficulty, and may make that there is preferable sealing effect between the lateral wall of the first canister portion 111 and the inner sidewall of the second box body 200. It of courses, in concrete application, as the embodiment of substitution, the lateral wall and the second box body in the first canister portion 111 can also be passed through The mode that sealing ring is arranged between 200 inner sidewall is realized between 200 inner sidewall of 111 lateral wall of the first canister portion and the second box body Sealing.

Preferably, the quantity of the first cyclic annular convex ribs 113 is at least two, in this way, may make 100 lateral wall of the first box body with Second box body, 200 inner sidewall has at least two seal points in the axial direction, is conducive to improve 100 lateral wall of the first box body and the second box Sealing effect between 200 inner sidewall of body.As a preferred embodiment of the present embodiment, the first cyclic annular convex ribs 113 are equipped with four It is a, it of courses, in concrete application, the quantity of the first cyclic annular convex ribs 113 is without being limited thereto.

Preferably, referring to shown in Fig. 2 and Fig. 5, hollow housing 110 further includes below the first canister portion 111 and outer diameter is small The second canister portion 114 in 111 outer diameter of the first canister portion and the taper canister portion that is connected between the first canister portion 111 and the second canister portion 114 115.Herein, the end portion of hollow housing 110 is designed as the lesser structure of outer diameter, so that under hollow housing 110 End part will not influence the assembly difficulty of the first box body 100 and the second box body 200, in this way, both may make the axis of hollow housing 110 It is obtained to Design of length longer, and the first box body 100 and the assembly manipulation of the second box body 200 may make to be relatively easy to realize.Conically shaped The design in portion 115 is mainly used for transition and connects the first canister portion 111 and the second canister portion 114, to may make the first canister portion 111, cone Shape canister portion 115, the second canister portion 114 may be designed as the identical structure of wall thickness, and then the manufacture for being conducive to reduce hollow housing 110 is difficult Degree, and be conducive to save material cost.

Preferably, the top in detent portion 112 is recessed with annular recess 116.In the detection process, when in detection kit When reagent, blood sample are splashed to the top of the first box body 100 out of first box body 100, even if having reagent, blood sample along the first box body 100 top inner sides are flowed to the outer edge of the first box body 100, then also can be by 100 outer edge flow agents of the first box body, blood sample It is collected into annular recess 116, without flowing directly out outside detection kit, to be conducive to be better protected from detection process Reagent, blood sample endanger phenomenon to the biological infection of operator.

Preferably, referring to shown in Fig. 3, Fig. 4 and Fig. 7, the bottom of box cover 300 be convexly equipped with for across diaphragm seal 130 and in Empty capsid 110 simultaneously opens sealing-plug 120 the body of rod 310 of the first box body 100 and the second box body 200 is connected.In detection process In, after box cover 300 is installed on the first box body 100 from the top of the first box body 100, continue to press box cover 300, the body of rod down 310 can open sealing-plug 120, so that the conducting of the first box body 100 and the second box body 200 is realized, so that the first box body 100 In blood sample, the first reagent 500 is flowed into the second box body 200 and mixes with the second reagent 600.Herein, 300 bottom bar of box cover The design of body 310 is used so that in the detection process, directly can open sealing-plug 120 with box cover 300 without additional designs In the mechanism for opening sealing-plug 120.

Preferably, referring to shown in Fig. 3, Fig. 4 and Fig. 7, box cover 300 includes the body of rod 310, is used for and 110 card insertion of hollow housing The card insertion body 320 of cooperation, the cover board 330 for being convexly equipped in 320 top of card insertion body and being stuck in outside hollow housing 110 and be set to cover board 330 Outer edge and back-off 340 for being clasped with the outside portion of hollow housing 110, the body of rod 310 are convexly equipped in the bottom of card insertion body 320 End.The lateral wall of card insertion body 320 is connected to the inner sidewall of the first canister portion 111.Cover board 330 is used to cover the top of hollow housing 110 Portion's opening.When back-off 340 opens sealing-plug 120 in the detection process pressing box cover 300 down, box cover 300 can be fastened In on the first box body 100, to be conducive to improve the solid and reliable property that box cover 300 is connect with the first box body 100.

Preferably, referring to shown in Fig. 3, Fig. 4 and Fig. 7, cover board 330 includes flat part 331 and inclined plate portion 332, flat part 331 From the top of card insertion body 320 towards the straight extension in direction for deviating from 320 central axis of card insertion body, inclined plate portion 332 is remote from flat part 331 Edge from card insertion body 320 is extended obliquely downward towards the direction away from 320 central axis of card insertion body, back-off 340 from inclined plate portion 332 it Edge far from flat part 331 extends downwardly projection.The setting in inclined plate portion 332, so that the pressing of box cover 300 is assemblied in the first box body After on 100, inclined plate portion 332 can fit closely the first box body 100, to be conducive to improve the close of lid the first box body 100 of conjunction of box cover 300 Seal effect.

Preferably, 340 at least two are buckled to, and each back-off 340 is uniformly spacedly distributed along the outer edge of cover board 330.

Preferably, sealing-plug 120 is elastic plug, in this way, sealing-plug 120 is made to be installed on the bottom of hollow housing 110 With preferable sealing effect, and make sealing-plug 120 under external force, is relatively easy to be opened.

Preferably, referring to shown in Fig. 2, Fig. 4 and Fig. 5, sealing-plug 120 include for from 110 bottom card insertion of hollow housing in Cock body 121 in hollow housing 110 and the cock cap 122 for being convexly equipped in 121 one end of cock body and being stuck in outside hollow housing 110.Cock cap 122 Outer diameter be greater than cock body 121 outer diameter.The clamping matching of cock cap 122 and hollow housing 110 is, it can be achieved that install sealing-plug 120 Axial limiting on hollow housing 110.The cooperation of the card insertion of cock body 121 and hollow housing 110 is, it can be achieved that pacify sealing-plug 120 Loaded on the radial limit and the sealing of 121 lateral wall of cock body and 110 inner sidewall of hollow housing cooperation on hollow housing 110.

Preferably, cock body 121 cooperates with 110 card insertion of hollow housing in an interference fit.The outer diameter of cock body 121 is greater than The internal diameter of second canister portion 114.In the present embodiment, cock body 121 has elasticity, and cock body 121 passes through interference fit type and hollow shell Body 110 cooperates, and has not only been conducive to guarantee the sealing effect at cock body 121 and 110 cooperation of hollow housing, but also be convenient for the installation of cock body 121 With open.

Preferably, referring to shown in Fig. 2, Fig. 4 and Fig. 5, the lateral wall of cock body 121 is convexly equipped with several and sets along its axially spaced-apart It sets for the second cyclic annular convex ribs 123 with the sealing cooperation of the inner sidewall of hollow housing 110.In the present embodiment, cock body 121 passes through Second cyclic annular convex ribs 123 are realized to be cooperated with the tight fit of hollow housing 110 and sealing, be both conducive to reduce 121 lateral wall of cock body and The contact area of 110 inner sidewall of hollow housing, to be conducive to reduce the assembly difficulty of sealing-plug 120 and hollow housing 110, and can So that having preferable sealing effect between the lateral wall of cock body 121 and the inner sidewall of hollow housing 110.It of courses, specifically answers It, can also be by being set between the lateral wall of cock body 121 and the inner sidewall of hollow housing 110 as the embodiment of substitution in The mode for setting sealing ring realizes sealing between 110 inner sidewall of 121 lateral wall of cock body and hollow housing.

Preferably, the quantity of the second cyclic annular convex ribs 123 is at least two, in this way, may make 121 lateral wall of cock body and second 114 inner sidewall of canister portion has at least two seal points in the axial direction, is conducive to improve in 121 lateral wall of cock body and the second canister portion 114 Sealing effect between side wall.

Referring to shown in Fig. 8, in concrete application, can also detachably it be equipped in the bottom end of the body of rod 310 for taking blood sample Sample capillary 400, in this way, in a particular application, using the sample capillary 400 on box cover 300 directly from sufferer finger tip It samples, without individually blood drawing sampling, reduces the pain of injury of sufferer, and acquisition blood sample is few, technology low to the environmental requirement of acquisition blood sample It is low with operational risk.Blood sample refers specifically to blood sample.

Further, the embodiment of the invention also provides a kind of biochemical immunity detection methods comprising:

The first reagent 500 is loaded in the first box body 100 and seals the first box body 100, while being filled in the second box body 200 The second reagent 600 is carried, then 100 card insertion of the first box body is installed on the second box body 200；

By liquid transfer gun head, perhaps then sample capillary 400 takes blood sample with liquid transfer gun head or sample capillary 400 The diaphragm seal 130 on the first box body 100 is punctured, and makes liquid transfer gun head or blood sample and the first box body in sample capillary 400 The first reagent 500 mixing in 100；

Box cover 300 is installed on the first box body 100 from the top of the first box body 100, and presses box cover 300 down with logical It crosses box cover 300 and opens sealing-plug 120, so that the blood sample, the first reagent 500 in the first box body 100 are flowed into the second box body 200 It is interior and mixed with the second reagent 600；

Detection kit manually or by mechanical mechanism is shaken, to shake uniform blood sample, the first reagent 500 and the second examination Agent 600；

After will test kit standing a period of time, pass through detecting instrument interpretation testing result.

The biochemical immunity detection method of the embodiment of the present invention is specifically that the immune biochemistry combined occurs for two kinds of reagents and sample Reaction, when detecting, the first reagent 500 and the second reagent 600 that first detection is needed can be by outer by being encapsulated in one In the detection kit of boundary's force action, the present embodiment is that the is realized in detection kit by the puncture of box cover 300 force The mixing of one reagent 500 and the second reagent 600, and its sample-adding process for only needing primary sample that can complete entirely to detect. The biochemical immunity detection method of the embodiment of the present invention, due to only needing a liquid relief sample-adding, therefore, reduce the accumulative mistake being repeatedly loaded Difference reduces the fault risk of handwork, improves the accurate reliability and efficiency of detection；And detection kit constitutes one The detection environment of sealing has prevented the biological infection harm to operator；After the completion of detection, blood sample and reagent are existed with sealed storage The form of detection kit works as offal treatment, reduces collection, the processing difficulty of subsequent bio Biohazard Waste, and avoids It is generated in the collection of waste, treatment process and the biological infection of operator is endangered.

In the embodiment of the present invention, two kinds of reagents for participating in detection will be needed by being sealed to independent cavity in advance respectively In (the present embodiment is the first box body 100 and the second box body 200), after blood sample to be added, is acted by one, can will need to join Cavity where the two kinds of reagents reacted with detection is connected to, and then mixes blood sample and two kinds of reagents to carry out biochemistry together Reaction, some of sealing elements (the present embodiment is sealing-plug 120) act also as evenly mixing device, after the reaction was completed, entire liquid Waste sealed storage is discarded into biochemical wastes recycling vessel in detection kit.It only need to be once loaded in entire detection Afterwards, and subsequent action is easy to operate, operates convenient for a large amount of manual inspections, and automation continuous productive process, detection efficiency also may be implemented Height, it is simple to operate, it is particularly suitable for quickly detecting in (POCT) project.

As a preferred embodiment of the present embodiment, the packaging method of detection kit are as follows: in advance the same detection Test need the first reagent 500 and the second reagent 600 be encapsulated into the first box body 100 and the second box body 200 respectively, herein I The top of 100 hollow housing 110 of the first box body is first sealed up into hollow housing 110 with the special sealing aluminium foil easily punctured One detection project is needed the first reagent 500 of dosage from the bottom quantitative filling of hollow housing 110 to first by top opening In box body 100, then with sealing-plug 120 seal hollow housing 110 bottom opening (of course, can also be first in concrete application The bottom opening that the bottom sealing-plug 120 of first box body, 100 hollow housing 110 is sealed to hollow housing 110 detects one Project needs the first reagent 500 of dosage from the top quantitative filling of hollow housing 110 into the first box body 100, then with spy The different sealing aluminium foil easily punctured seals up the top opening of hollow housing 110), it then will test the second reagent for needing dosage 600 are quantitatively loaded onto the second box body 200, finally will be packaged and be mounted with the first box body 100 of the first reagent 500 Encapsulate the second box body 200.Different detection projects can by be posted in detection kit side two dimensional code (bar code) or Other mark automatic identifications.

The preferable operating method being used for quickly detecting using the detection kit of the present embodiment are as follows: will be by being mounted with inspection After the sealing aluminium foil for needing directly to wear out the top of the first box body 100 with the liquid transfer gun head of blood sample is tested in test, blood sample is filled into the In one box body 100 (or if directly sampled from sufferer finger tip using the sample capillary 400 on box cover 300, by pressing box It covers into the first box body 100, directly wears out the sealing aluminium foil at 100 top of the first box body, the finger tip blood sample in sample capillary 400 It is directly mixed with the first reagent 500 in the first box body 100)；Then by sealing up the first box body to the force pressing of box cover 300 100, meanwhile, the body of rod 310 of 300 bottom of box cover can open the sealing-plug 120 of 100 bottom of the first box body, so that 120 He of sealing-plug The first reagent 500, blood sample in first box body 100 are fallen in the second box body 200, artificial or mechanical agitation detection reagent The sealing-plug 120 of box, the second box body 200 and its inside shakes blood sample, the first reagent 500 and the second reagent for mixing and participating in reaction 600；Then the detection kit for being mounted with blood sample, the first reagent 500 and the second reagent 600 is put into the incubation room (ring of formulation Border) in, 3~detect simultaneously interpretation result, inspection by detecting instrument (diascope or other optical detecting instruments) after five minutes After survey, entire detection kit is discarded into biochemical wastes recycling vessel.

In the preferred embodiment of the present embodiment, realized by the additional force of box cover 300 by the first reagent 500 and the Hermetic barrier object (the present embodiment is sealing-plug 120) between two reagents 600 is got through.It of courses, in concrete application, can also lead to Other way realization is crossed to get through the hermetic barrier object between the first reagent 500 and the second reagent 600, such as: first using external Mechanism open sealing-plug 120, then the first box body 100 is covered with box cover 300, alternatively, can also beat using biochemical chemical reaction Hermetic barrier object between logical first reagent 500 and the second reagent 600.

Detection kit provided in an embodiment of the present invention and biochemical immunity detection method are used in quick diagnosis (POCT), especially It is can to obtain following remarkable result in the detection for need repeatedly to add in reaction process different reagents: down-sample the time, mention High detection efficiency reduces the accumulative sampling error repeatedly sampled, improves the accurate reliability of detection, prevents to carry in detection dirty Dye, while solving the problems, such as the series techniques such as biochemical harm of waste after reacting.

The above description is only a preferred embodiment of the present invention, is not intended to limit the scope of the invention, all at this Under the inventive concept of invention, using equivalent structure transformation made by description of the invention and accompanying drawing content, or directly/use indirectly It is included in other related technical areas in scope of patent protection of the invention.

Claims (10)

1. detection kit, which is characterized in that including for accommodating the first reagent the first box body, for accommodating the second reagent Second box body, the box cover being installed at the top of first box body for covering conjunction, first box body include described in bottom can be inserted into Hollow housing that in second box body and top can be stuck in outside second box body, the sealing for sealing the hollow housing bottom It fills in, for sealing the diaphragm seal at the top of the hollow housing.

2. detection kit as described in claim 1, which is characterized in that the hollow housing includes card insertion in second box In vivo and lateral wall and the second box body inner sidewall sealing cooperation the first canister portion and be convexly equipped in first canister portion top and It is stuck in the detent portion outside second box body.

3. detection kit as claimed in claim 2, which is characterized in that the lateral wall of first canister portion is convexly equipped with several It is arranged along its axially spaced-apart for the first cyclic annular convex ribs with the second box body inner sidewall sealing cooperation；And/or

The hollow housing further includes below first canister portion and outer diameter is less than second of the first canister portion outer diameter Portion and the taper canister portion being connected between first canister portion and second canister portion；And/or

Annular recess is recessed at the top of the detent portion.

4. detection kit as described in any one of claims 1 to 3, which is characterized in that the bottom projection of the box cover is useful In passing through the diaphragm seal and the hollow housing and open the sealing-plug first box body and described second is connected The body of rod of box body.

5. detection kit as claimed in claim 4, which is characterized in that the box cover include the body of rod, for it is described It the card insertion body of hollow housing card insertion cooperation, the cover board that is convexly equipped in card insertion body top and is stuck in outside the hollow housing and is set to The cover board outer edge and back-off for being clasped with the outside portion of the hollow housing, the body of rod are convexly equipped in the card Insert the bottom end of body.

6. detection kit as claimed in claim 5, which is characterized in that the cover board includes flat part and inclined plate portion, described Flat part is from the top of the card insertion body towards the straight extension in direction for deviating from the card insertion body central axis, and the inclined plate portion is from described The edge far from the card insertion body of flat part is extended obliquely downward towards the direction away from the card insertion body central axis, the back-off Projection is extended downwardly from the edge far from the flat part in the inclined plate portion.

7. detection kit as described in any one of claims 1 to 3, which is characterized in that the sealing-plug includes for from institute Hollow housing bottom card insertion is stated in the intracorporal cock body of the hollow shell and is convexly equipped in described cock body one end and is stuck in the hollow shell External cock cap, the cock body cooperate with the hollow housing card insertion in an interference fit.

8. detection kit as claimed in claim 7, which is characterized in that the lateral wall of the cock body is convexly equipped with several along it Axially spaced-apart setting is for the second cyclic annular convex ribs with the sealing cooperation of the inner sidewall of the hollow housing.

9. detection kit as described in any one of claims 1 to 3, which is characterized in that the diaphragm seal be metal foil paper or Person's metal foil；And/or

The sealing-plug is elastic plug；And/or

The bottom end of the box cover is detachably equipped with the sample capillary for taking blood sample.

10. biochemical immunity detection method characterized by comprising

The first reagent is loaded in the first box body and seals the first box body, while loading the second reagent in the second box body, then The first box body card insertion is installed on second box body；

By liquid transfer gun head, perhaps sample capillary takes blood sample then to be pierced with the liquid transfer gun head or the sample capillary The diaphragm seal on first box body is broken, and makes the blood sample and first box body in the liquid transfer gun head or sample capillary In first reagent mixing；

Box cover is installed on first box body at the top of first box body, and presses the box cover down to pass through It states box cover and opens the sealing-plug, so that the blood sample, first reagent in first box body are flowed into described It is mixed in two box bodys and with the second reagent；

Manually or the detection kit is shaken by mechanical mechanism, to shake the uniformly blood sample, the first reagent and second Reagent；

After the detection kit is stood a period of time, pass through detecting instrument interpretation testing result.