CN215947252U

CN215947252U - Liquid sample processing device

Info

Publication number: CN215947252U
Application number: CN202121734154.5U
Authority: CN
Inventors: 韩亚平; 赵譞; 张淼; 顾城玮; 臧百胜
Original assignee: Jijian Medical Technology Suzhou Co ltd
Current assignee: Beijing Jijian Medical Technology Co ltd
Priority date: 2021-07-28
Filing date: 2021-07-28
Publication date: 2022-03-04
Anticipated expiration: 2031-07-28

Abstract

The utility model discloses a liquid sample processing device, which relates to the technical field of experimental equipment and comprises a box body made of non-magnetic conductive materials, wherein at least 2 reagent reaction grooves are arranged in the box body, the open ends of the at least 2 reagent reaction grooves are communicated through a common cavity, and each reagent reaction groove is at least provided with 1 reagent injection port communicated with the outside. The liquid sample processing device provided by the utility model has the advantages of high efficiency and pollution prevention, and can be matched with full-automatic detection equipment for use.

Description

Liquid sample processing device

Technical Field

The utility model relates to the technical field of experimental equipment, in particular to a liquid sample processing device.

Background

The existing detection of target nucleic acid molecules is to sample human or environment and then detect whether nucleic acid small molecule fragments exist in liquid capable of containing the nucleic acid small molecule fragments (such as virus preservation liquid soaked with throat swabs or human blood).

One method is to take a sample from the body (or environment) and test a portion of it by (qPCR) amplification to determine whether the sample contains fragments. Such a test method has certain disadvantages because the target virus may not be obtained due to unevenness or operation error during the process of separating the sample, especially when the virus content in the original sample is small (because it cannot be regarded as a completely uniform solution).

Another method is concentration enrichment. In addition to direct sampling of (a portion of) the sample for detection, methods of extraction and enrichment may also be employed. In short, the "column extraction method" can be used to grasp all the nucleic acid molecules in the liquid, and the "magnetic bead method" can also be used because the magnetic beads have flexible moving mode and huge specific surface area, and the magnetic beads are put away to actively grasp DNA and then are collected. For example, in one of the specific operation modes, functional groups such as hydroxyl group and amino group are synthesized on the surface of the magnetic bead, and then the magnetic bead is further modified to connect a specially designed specific probe, so that the ability of "fishing" the gene fragment of interest in the solution is possible, and then the magnetic bead is collected back by the action of the magnetic field, so that the magnetic bead is separated from the bulk liquid and aggregated, and the molecular fragment of interest is refined with high concentration for further processing or reaction (such as PCR amplification).

The above detection method involves soaking and cleaning with multiple reagents, and in the prior art, magnetic beads are generally transferred among multiple containers containing different reagents, or different reagents are sequentially replaced in the same container, but both methods are time-consuming and labor-consuming, and many chances of contaminating samples exist in the process of transferring magnetic beads or replacing reagents, so that a detection method with higher efficiency and fewer chances of contamination is urgently needed.

SUMMERY OF THE UTILITY MODEL

The utility model aims to solve the defects in the prior art and provides a liquid sample processing device.

In order to achieve the purpose, the utility model adopts the following technical scheme:

the liquid sample processing device comprises a box body made of non-magnetic materials, wherein at least 2 reagent reaction grooves are formed in the box body, the open ends of the at least 2 reagent reaction grooves are communicated through a common cavity, and each reagent reaction groove is at least provided with 1 reagent injection port communicated with the outside.

Furthermore, the reagent reaction tank comprises a head end reaction tank, a tail end reaction tank and a plurality of middle reaction tanks, wherein the head end reaction tank is also provided with a liquid sample injection port, and the tail end reaction tank is used as a PCR reaction site.

Still further, the reaction tank further comprises a reagent injection cavity which is communicated with the reaction tank through the reagent injection port.

Furthermore, a capsule matched with the reagent injection cavity is also arranged, and the capsule or the reagent injection port is provided with a quick-wear port device.

Furthermore, the box body is provided with an upper box body and a lower box body which are movably connected, the reagent reaction groove is arranged on the upper box body, and the upper box body and the lower box body are respectively provided with a groove; when the upper box body is combined with the lower box body, the grooves of the upper box body and the lower box body are matched to form a reagent injection cavity.

Furthermore, a piston is arranged in the capsule, and a push rod of the piston extends out of the box body.

Furthermore, magnetic beads are arranged in the reagent reaction tank or the capsule, and the inner wall of the card box is a smooth surface.

Furthermore, the box body is also provided with an air outlet communicated with the outside air, and the air outlet is provided with a filter element only allowing the air to pass through.

Further, the volume of the reagent reaction groove is between 1 microliter and 5000 microliter.

Furthermore, the PCR reaction site is in one of a tubular shape, a flat sheet shape and a long strip shape, and the internal volume is 1 microliter to 500 microliter.

Compared with the prior art, the utility model has the beneficial effects that:

the liquid sample processing device provided by the utility model can grab, enrich and extract target small molecular fragments in liquid, and does not need to use large-volume liquid or large-volume liquid sample for experiment; the limitation caused by taking small-volume liquid from a large-volume liquid sample for experiment is also avoided; the magnetic beads are arranged to carry target molecule fragments and can be transferred from one liquid environment to another liquid environment in the closed space; the reaction chamber is provided with an integrally formed sealing structure, the reaction chamber is provided with an air outlet communicated with the outside air, and the air outlet is provided with a filter element plug, so that the effect of only allowing air to pass (blocking aerosol) is achieved, the closed space in the upper box body is prevented from being polluted by impurities in the outside environment, and meanwhile, the gas generated in the reaction chamber is prevented from leaking to the outside to pollute the environment; through the capsule provided, to inject different types of reagents into the reaction chamber.

Drawings

The accompanying drawings, which are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this specification, illustrate embodiments of the utility model and together with the description serve to explain the principles of the utility model and not to limit the utility model.

FIG. 1 is a schematic view of an overall structure of a liquid sample processing apparatus according to the present invention;

FIG. 2 is a schematic structural diagram of an upper case according to the present invention;

fig. 3 is a schematic structural diagram of a lower case according to the present invention.

In the figure: 1. a capsule;

2. a reagent injection chamber;

3. an upper box body;

31. a reaction tank at the head end;

32. a common cavity;

33. an intermediate reaction tank;

34. a terminal reaction tank;

35. a needle; 4. magnetic beads; 5. a lower box body; 6. a piston.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.

In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.

Referring to fig. 1-3, a liquid sample processing device comprises a box body of non-magnetic conductive material and a capsule 1, wherein the box body is provided with an upper box body 3 and a lower box body 5 which are movably connected, a reagent reaction groove is arranged in the upper box body 3, and the upper box body 3 and the lower box body 5 are respectively provided with a groove; when the upper box body 3 is combined with the lower box body 5, the grooves of the upper box body and the lower box body are matched to form the reagent injection cavity 2, when the device is used, the capsule is firstly placed in the groove of the lower box body 5, and when the upper box body and the lower box body are combined, the capsule is locked and positioned.

In other embodiments, the box body can also be of an integral structure, only one groove is arranged at the bottom of the box body to serve as the reagent injection cavity 2, the tenon clamping device is arranged in the reagent injection cavity 2 and matched with the capsule, and the capsule can be locked and positioned after being inserted into the reagent injection cavity 2 during use.

Reagent reaction grooves are arranged in the box body, the reagent reaction grooves comprise a head end reaction groove 31, a tail end reaction groove 34 and a plurality of middle reaction grooves 33, the opening ends of the reagent reaction grooves are communicated through a common cavity 32, and each reagent reaction groove is at least provided with 1 reagent injection port communicated with the outside; when in use, any existing injection device can be used for injecting the reagent or other liquid into the reaction tank, in the embodiment, in order to be more suitable for a full-automatic machine, the reagent injection port is arranged to be communicated with the reagent injection cavity 2; the reagent reaction tanks can be in any geometric shape (as long as the reagent reaction tanks can effectively bear liquid), are communicated with each other, are not isolated and can transfer substances, and a plurality of reaction tanks can be arranged linearly, in parallel or in a ring shape; it can be understood that: when the magnetic bead is used, the magnetic bead is used.

The capsule 1 is matched with the reagent injection cavity 2, and the upper end of the capsule 1 or the reagent injection port is provided with a vulnerable port which can be a soft plugging object such as a sealing film, a prefabricated crack, paraffin and the like or any other prior art. When the capsule is provided with a vulnerable port, a puncture needle 35 with a hollow structure is arranged at the opening of the reagent injection cavity 2; when a vulnerable port is arranged at the reagent injection port, a puncture needle 35 with a hollow structure is arranged at the upper end of the capsule. The working principle is that the puncture needle 35 with a hollow structure punctures a vulnerable port to realize communication.

The capsule 1 is a tubular object (the section shape can be square, round, irregular and the like) with or without a piston 6 and a sealing film, if the capsule 1 is provided with the piston 6, the piston 6 is arranged at the bottom of the capsule 1 and can freely move towards the inside or the outside of the capsule under the action of external force, wherein a push rod of the piston 6 extends out of the box body, the capsule 1 is provided with a smooth inner surface for the piston 6 to slide smoothly, and reagents in various forms such as liquid, freeze-dried powder, freeze-dried microspheres or glass state and the like can be stored in the capsule 1;

wherein, the head end reaction tank 31 is also provided with a liquid sample injection port, the tail end reaction tank 34 is used as a PCR reaction site, the volume of each reagent reaction tank is between 1 microliter and 5000 microliter, and the PCR reaction site is in the shape of one of a tube, a flat sheet and a strip, and the internal volume is between 1 microliter and 500 microliter;

the two injection ports are embedded and blocked by solid or liquid biochemical reagents (including but not limited to paraffin, alkane, silicon oil, grease and mixture thereof) when necessary;

in other embodiments, the reagent reaction chamber or capsule 1 is provided with magnetic beads 4, as will be appreciated by those skilled in the art: when the magnetic beads 4 are provided in the capsule, some of the magnetic beads cannot enter the reaction tank when being pressed, and therefore, a margin needs to be provided to compensate.

The upper box body 3 is also provided with an air outlet communicated with the outside air, the air outlet is provided with a filter element only allowing air to pass through, and the effect of only allowing air to pass through (blocking aerosol) is achieved, so that the pollution of impurities in the outside environment to the closed space in the upper box body 3 is prevented, and meanwhile, the gas generated in the closed space in the upper box body 3 is prevented from leaking to the outside to pollute the environment; these structures are prior art and their specific configuration is not described in detail.

The sealing mode of the upper box body 3 can be formed by buckling an end cover through injection molding (such as an EP pipe in the prior art), can also be a later-stage connecting mode of sealing a port by a film forming material (organic solvent bonding, thermal bonding, chemical bonding, physical bonding of pressure-sensitive double-sided adhesive tape), welding, adhering, adsorbing and the like, and can also be a plastic part sealing fastening mode and a screwing mode which are integrally processed and formed in the earlier stage.

The working principle and the using process of the utility model are as follows:

1. taking a capsule, putting the capsule into a liquid sample, treating the liquid sample by a vortex crusher for 2-4 minutes, and taking out the capsule so as to crush cells suspended in the liquid sample and release various nucleic acid molecules into the liquid.

2. Insert the capsule on the box body with in the head end reaction tank 31 reagent injection chamber 2 that corresponds, priming device or manpower on the machine promote the piston push rod, from by capsule 1 bottom, inject the reagent in the capsule 1 to the head end reaction tank 31 in.

3. Magnets are attached to two side faces of the box body, the magnets are used for driving the magnetic beads 4 to move, the magnetic beads 4 are mixed with liquid in the reaction tank at the head end, after being uniformly mixed, the mixture is gathered under the attraction of the magnets, then the mixture is led to be transferred to other reagent reaction tanks, and then other reagents are injected and mixed;

4. transferring the magnetic beads 4 into a terminal reaction tank 34 serving as a PCR reaction site, arranging a capsule 1 with a reagent solution below the reagent reaction tank, and injecting the capsule 1 to mix the solution in the capsule 1 with the magnetic beads 4 for PCR reaction;

5. after the reaction is finished, detecting signals by using a photoelectric detection system, and reporting the detection result.

In the process of the step 3-4, the inner space of the upper box body can be heated by the heating device if necessary, so that the temperature of the liquid in the inner space of the upper box body is raised, and after the heating is finished, the temperature of the liquid in the inner space of the upper box body is reduced by the cooling device.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and equivalent alternatives or modifications according to the technical solution of the present invention and the inventive concept thereof should be covered by the scope of the present invention.

Claims

1. The liquid sample processing device is characterized by comprising a box body made of a non-magnetic conductive material, wherein at least 2 reagent reaction grooves are arranged in the box body, the open ends of the at least 2 reagent reaction grooves are communicated through a common cavity, and each reagent reaction groove is at least provided with 1 reagent injection port communicated with the outside.

2. The apparatus of claim 1, wherein the reagent reaction tank comprises a first reaction tank, a last reaction tank and a plurality of intermediate reaction tanks, wherein the first reaction tank is further provided with a liquid sample injection port, and the last reaction tank serves as a PCR reaction site.

3. The liquid sample processing device according to claim 2, further comprising a reagent injection chamber, wherein the reagent injection chamber is communicated with the reaction tank through the reagent injection port.

4. A liquid sample processing device according to claim 3, further provided with a capsule cooperating with said reagent injection chamber, said capsule or said reagent injection port being provided with a frangible port means.

5. The liquid sample processing device according to claim 3 or 4, wherein the box body is provided as an upper box body and a lower box body which are movably connected, the reagent reaction tank is provided on the upper box body, and the upper box body and the lower box body are respectively provided with a groove; when the upper box body is combined with the lower box body, the grooves of the upper box body and the lower box body are matched to form a reagent injection cavity.

6. The liquid sample processing device of claim 4, wherein a piston is disposed in the capsule, and a push rod of the piston extends out of the cartridge body.

7. The device for processing liquid samples according to claim 4, wherein magnetic beads are disposed in the reagent reaction tank or the capsule, and the inner wall of the cartridge body is a smooth surface.

8. A liquid sample treatment device according to any of claims 1 to 4, wherein the housing is further provided with an air outlet in communication with the ambient air, the air outlet being provided with a filter element for allowing air only to pass therethrough.

9. The liquid sample processing device according to any of claims 1 to 4, wherein the volume of said reagent reaction chamber is between 1 microliter and 5000 microliter.

10. The liquid sample processing device according to claim 2, wherein the PCR reaction site is in the form of one of a tube, a flat sheet and a strip, and has an internal volume of 1 to 500. mu.l.