CN219490025U - Nucleic acid detection and analysis kit - Google Patents
Nucleic acid detection and analysis kit Download PDFInfo
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- CN219490025U CN219490025U CN202320934203.2U CN202320934203U CN219490025U CN 219490025 U CN219490025 U CN 219490025U CN 202320934203 U CN202320934203 U CN 202320934203U CN 219490025 U CN219490025 U CN 219490025U
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The utility model provides a nucleic acid detection and analysis kit, which relates to the technical field of biological detection and comprises the following components: the central shaft is provided with a central flow passage, a suction module is arranged in the central flow passage, and a groove is further formed in the central shaft; the main box is sleeved outside the central shaft and can rotate around the shaft, so that one of the liquid separation channel and each cavity is communicated with the central flow channel, the top of each cavity is sealed in an initial state, and the main box is in sealing connection with the central shaft; and a base configured with a magnetic attraction module for receiving the magnetic beads into the grooves. The method solves the problems that in the prior art, sample pretreatment is still needed, the operation is complex, the risk of cross contamination exists in the sample transferring process, and the sample detection precision is reduced.
Description
Technical Field
The utility model relates to the technical field of biological detection, in particular to a nucleic acid detection and analysis kit.
Background
The nucleic acid detection is widely applied to the fields of disease detection, biological analysis, biological medicine and the like. The detection process comprises sampling a sample, preprocessing the sample (sample cracking, washing, drying and eluting), extracting nucleic acid, preparing a reagent, amplifying and the like, and in the nucleic acid detection process, the preprocessed sample is transferred into a PCR reaction tube for detection.
The above steps of the current nucleic acid detection are mainly performed manually or part of the flow is completed by a semi-automatic instrument, but the problems of sample pretreatment, complex operation and cross contamination risk in the sample transfer process still exist, and the precision of the sample detection is reduced.
Disclosure of Invention
Aiming at the defects existing in the prior art, the utility model aims to provide a nucleic acid detection analysis kit which solves the problems that in the prior art, sample pretreatment is still needed, the operation is complex, the risk of cross contamination exists in the sample transferring process, and the sample detection precision is reduced.
According to an embodiment of the present utility model, a nucleic acid detection assay kit includes:
the central shaft is provided with a central flow passage, a suction module is arranged in the central flow passage, and a groove is further formed in the central shaft;
the main box is sleeved outside the central shaft and can rotate around the shaft, so that one of the liquid separation channel and each cavity is communicated with the central flow channel, the top of each cavity is sealed in an initial state, and the main box is in sealing connection with the central shaft;
and a base configured with a magnetic attraction module for receiving the magnetic beads into the grooves.
Compared with the prior art, the utility model has the following beneficial effects:
on one hand, the kit integrates the processes of nucleic acid extraction, reagent preparation and the like into one kit, so that sample pretreatment is not needed, after a sample or cell tissue is directly injected into a cracking cavity during use, the processes of sample pretreatment, nucleic acid extraction, reagent preparation, amplification and the like can be realized by gradually rotating the main kit, sample inlet and outlet are realized, a molecular full-automatic process is realized, and the operation is convenient; on the other hand, the whole detection process is in a closed kit, so that multiple cross contamination and reagent leakage are prevented, and the risk of leakage of nucleic acid aerosol, samples and reagents is avoided.
Further, a vertically penetrating mounting hole is formed in the center of the main box, and a platform is arranged at the bottom of the main box;
the central shaft includes:
the shaft body is in a hollow cylindrical shape and penetrates through the mounting hole;
and the pressure plate is fixedly arranged at the bottom of the shaft body, the top end face of the pressure plate is attached to the bottom end face of the platform, a sealing gasket is pressed between the top end face of the pressure plate and the bottom end face of the platform, a radial channel communicated with the inner cavity of the shaft body is arranged on the pressure plate, the other end of the radial channel can be alternatively communicated with the liquid separation channel and various cavities, and a vertically through air hole is formed in the top end of the pressure plate.
Further, the drainage post that is used for joint detection tube has been set firmly to the bottom of main box, divide the liquid passageway to include:
one end of the reaction liquid flow passage can be communicated with the central flow passage, and the other end of the reaction liquid flow passage is communicated with the drainage column;
and one end of the ventilation channel is communicated with the drainage column, and when one end of the reaction liquid flow channel is communicated with the central flow channel, the other end of the ventilation channel is communicated with the ventilation hole.
Further, the main box is provided with a sinking platform, the liquid separation channels are provided with a plurality of liquid separation channels and are all arranged on the sinking platform, and the top of each liquid separation channel is open and sealed through a coating.
Further, the radial channel is arranged on the bottom end face of the pressure plate, and the radial channel further comprises a central pressing piece which is fixedly arranged on the bottom end face of the pressure plate and used for sealing the radial channel.
Further, the pressure plate is provided with a clamping groove, the base is cylindrical and sleeved outside the main box, and the base is fixedly provided with a blocking strip clamped in the clamping groove.
Further, the top end of the shaft body extends out of the main box, a clamping hole is formed in the end of the shaft body, the shaft body further comprises a gland, and a buckle capable of being clamped into the clamping hole is arranged on the gland.
Further, the main box is further provided with a vent hole, and in an initial state, the central runner is communicated with the vent hole.
Further, the drying device also comprises a paraffin oil cavity which can be communicated with the central runner, wherein the paraffin oil cavity and the elution cavity are positioned on the same circumference, and the paraffin oil cavity is positioned on one side of the elution cavity, which is away from the drying cavity.
Drawings
FIG. 1 is a schematic diagram of the overall structure of an embodiment of the present utility model;
FIG. 2 is a schematic view of the internal structure of FIG. 1;
FIG. 3 is a schematic view of the central shaft structure in FIG. 1;
FIG. 4 is a schematic view of the structure of FIG. 3 at a bottom view angle;
FIG. 5 is a schematic view of the main box in FIG. 1;
FIG. 6 is a schematic view of the structure of FIG. 5 at a bottom angle;
fig. 7 is a top view of fig. 5.
In the above figures:
101. a shaft body; 102. a pressure plate; 1021. a radial passage; 1022. ventilation holes; 1023. a clamping groove; 1024. a suction hole; 1025. a groove;
2. a suction module;
3. a main box; 301. a platform; 302. a sinking platform; 303. a vent hole;
4. cracking the cavity; 5. combining the die cavity; 6. rinsing the cavity; 7. drying the cavity; 8. eluting the cavity;
9. a liquid separation channel; 901. a reaction liquid flow channel; 902. a ventilation channel;
10. a base; 11. a sealing gasket; 12. a drainage column; 13. a detection tube; 14. tabletting at the center; 15. a gland; 16. paraffin oil cavity.
Detailed Description
The technical scheme of the utility model is further described below with reference to the accompanying drawings and examples.
The utility model provides an embodiment of a nucleic acid detection and analysis kit, as shown in fig. 1, 2 and 7, comprising: a central shaft, a main box 3 and a base 10, wherein,
referring specifically to fig. 3 and 4, the central shaft has a central flow channel, the central flow channel is internally provided with a suction module 2, and the central shaft is also provided with a groove 1025; for convenience of understanding, the description will be made herein with reference to a specific structure of a central shaft, where the central shaft includes a shaft body 101 and a pressure plate 102, the shaft body 101 is hollow and cylindrical, and the suction module 2 is, for example, a piston rod, and is disposed inside the shaft body 101; the top end surface of the pressure plate 102 is fixedly arranged at the bottom of the shaft body 101, a groove 1025 which is recessed downwards and used for accommodating magnetic beads is formed in the top end surface of the pressure plate 102, a radial channel 1021 which is communicated with a cavity of the shaft body 101 is formed in one side of the pressure plate 102 away from the shaft body 101, the radial channel 1021 and the cavity of the shaft body 101 form a central flow channel together, one end of the radial channel 1021 away from the shaft body 101 extends upwards and penetrates out of the pressure plate 102 to form a suction hole 1024, the suction hole 1024 can be communicated with a liquid separation channel 9 and various cavities alternatively, and a vertically through ventilation hole 1022 is formed in the top end of the pressure plate 102;
referring to fig. 5 and 6 specifically, the main box 3 is cylindrical, and a vertically penetrating mounting hole is arranged in the center of the main box 3, and the inner diameter of the mounting hole is matched with the outer diameter of the shaft body 101 for penetrating and mounting the shaft body 101; the bottom of the main box 3 is provided with a platform 301, a cracking cavity 4, a combining cavity 5, a rinsing cavity 6, a drying cavity 7, an eluting cavity 8 and a liquid separating channel 9 are sequentially distributed on the main box 3 around the circumference direction of the central shaft, of course, corresponding cracking liquid, rinsing liquid, drying agent, eluent and the like are added in each cavity in advance, and magnetic beads are also added in the cracking cavity 4; the liquid separation channels 9 are provided with a plurality of vertically penetrating cavities, and four liquid separation channels 9 are shown in the drawing of the embodiment, and of course, the number of the liquid separation channels is not limited and can be increased or decreased according to actual requirements; the main box 3 is sleeved outside the central shaft and can rotate around the central shaft, so that the liquid separation channel 9 and one of the various cavities are communicated with the suction hole 1024 of the central flow channel, and the top of each cavity is closed in an initial state (namely, the top of each cavity is closed, and a covering film at the top of the cracking cavity 4 is required to be pierced to inject samples when in use, as described in detail below); the main box 3 is in sealing connection with the central shaft, and in addition, a vent hole 303 is arranged on the main box 3, and in an initial state, the central flow channel is communicated with the vent hole 303, so that a piston in the central shaft can move up and down conveniently;
the base 10 is cylindrical, and a magnetic attraction module is configured in the base, and the magnetic attraction module can be an existing electromagnet device, and is positioned at the bottom of the pressure plate 102 to attract magnetic beads into the groove 1025 as required.
By adopting the scheme, on one hand, the kit integrates nucleic acid extraction, reagent preparation and the like into one kit, sample pretreatment is not needed, after a sample or cell tissue is directly injected into the cracking cavity 4 during use, the processes of sample pretreatment, nucleic acid extraction, reagent preparation, amplification and the like can be realized by gradually rotating the main box 3, sample inlet and outlet are realized, a molecular full-automatic process is realized, and the operation is convenient; on the other hand, the whole detection process is in a closed kit, so that multiple cross contamination and reagent leakage are prevented, and the risk of leakage of nucleic acid aerosol, samples and reagents is avoided.
In another embodiment of the present utility model, a structure of one of the liquid separation channels 9 is provided on the basis of the above embodiment, as shown in fig. 5 and 6, specifically, a cylindrical drainage column 12 is fixedly arranged at the bottom of the main box 3, and the outer diameter of the drainage column is matched with the inner diameter of the detection tube 13 so as to facilitate the clamping of the detection tube 13 on the drainage column 12; the liquid separation channel 9 includes: a reaction liquid flow passage 901 and a ventilation passage 902, wherein,
one end of the reaction liquid flow passage 901 and the suction hole 1024 are positioned on the same circumference so as to ensure that the main box 3 can be communicated with the central flow passage after rotating, and the other end of the reaction liquid flow passage is communicated with the drainage column 12;
one end of the ventilation channel 902 is communicated with the drainage column 12, and when one end of the reaction liquid flow channel 901 is communicated with the central flow channel, the other end of the ventilation channel 902 is communicated with the ventilation holes 1022; during liquid separation, the piston rod moves downwards, so that eluent in the cavity of the shaft body 101 can be sequentially injected into the liquid separation pipe through the reaction liquid flow channel 901 and the drainage column 12, meanwhile, air in the liquid separation pipe is extruded upwards, is discharged through the ventilation channel 902 and is finally discharged from the ventilation holes 1022, and the liquid separation process is simple and convenient.
In another embodiment of the present utility model, the arrangement mode of the liquid separation channels 9 is further optimized based on the above embodiment, as shown in fig. 5, specifically, a part of the main box 3 is recessed downwards to form a sinking platform 302, each liquid separation channel 9 is disposed on the sinking platform 302, each liquid separation channel 9 is recessed downwards, in practice, a layer of film is covered on the top of the liquid separation channel 9, so that the liquid separation channel 9 can be sealed, and the forming and manufacturing are convenient.
In another embodiment of the present utility model, a radial channel 1021 is provided on the basis of the above embodiment, as shown in fig. 2 and fig. 4, the radial channel 1021 is formed by recessing upward at the bottom of the platen 102, the annular side of the platen 102 extends downward, and correspondingly, a central pressing sheet 14 is further provided, which is embedded at the bottom of the platen 102, so as to seal the bottom of the radial channel 1021, and ensure that the liquid flows along the radial channel 1021 only without leakage, and meanwhile, the radial channel 1021 has a simple structure and is convenient to manufacture and process.
In another embodiment of the present utility model, a connection manner between the pressure plate 102 and the base 10 is provided on the basis of the above embodiment, as shown in fig. 1 and fig. 3, a clamping groove 1023 is provided on the ring side of the pressure plate 102, the base 10 is sleeved outside the main box 3, a blocking strip clamped in the clamping groove 1023 is fixedly provided on the base 10, and the blocking strip limits the rotation of the pressure plate 102 when the main box 3 rotates, so as to ensure that the central shaft remains motionless; in addition, the inner wall of the base 10 contacts with the outer wall of the main box 3, plays a role in guiding and limiting the main box 3, and ensures the coaxiality of the main box 3, the base 10 and the central shaft.
In another embodiment of the present utility model, a sealing connection manner between a central shaft and a main box 3 is provided on the basis of the above embodiment, as shown in fig. 1 and fig. 3, the top end of a shaft body 101 extends out of the main box 3, and the top end is provided with a plurality of clamping holes, which are distributed on the ring side of the shaft body 101, and the sealing device further comprises a gland 15, wherein the inner wall of the gland is provided with a buckle capable of being clamped into the clamping hole, the gland 15 is sleeved on the shaft body 101 to press downwards, so that the buckle is clamped into the clamping hole, the central shaft is limited to move up and down, and the sealing gasket 11 between the platform 301 and the pressure plate 102 is ensured to be in a compressed state, and the sealing device has a simple structure and is convenient to install; of course, the locking method of the gland 15 and the shaft body 101 is not limited to the above, and for example, it may be that an external thread is provided on the outer wall of the shaft body 101, and an internal thread is provided on the inner wall of the gland 15, so as to realize threaded connection of the gland 15 and the shaft body 101.
In another embodiment of the present utility model, the structure of the kit is further optimized based on the above embodiment, as shown in fig. 1, the kit further comprises a paraffin oil cavity 16, the bottom of which is vertically penetrated and can be communicated with the suction hole 1024 of the central runner, the paraffin oil cavity 16 and the elution cavity 8 are on the same circumference, and the paraffin oil cavity 16 is positioned at one side of the elution cavity 8 away from the drying cavity 7; paraffin oil is preset in the cavity.
The base 10 is also provided with an oscillation module (such as an existing ultrasonic oscillator) for mixing, and a heating module (also in the prior art, which is not described here in detail) for heating, and the use process of the nucleic acid detection and analysis kit of the utility model is as follows:
the thin film at the top of the cracking cavity 4 is punctured, a sample or cell tissue is injected into the cracking cavity 4, the oscillation module and the heating module are started, after uniform mixing and heating reaction, the magnetic attraction module is started to adsorb magnetic beads in the cracking cavity 4 into the groove 1025;
fixing a central shaft, rotating the main box 3 clockwise to enable the combined cavity 5 to be aligned with the groove 1025, starting the oscillation module to mix magnetic beads in the groove 1025 uniformly, and starting the magnetic attraction module to adsorb the magnetic beads in the groove 1025 after the mixed magnetic beads react in the combined cavity 5;
the central shaft is fixed, the main box 3 is rotated clockwise, the rinsing cavity 6 is aligned to the groove 1025, the oscillating module is started to uniformly mix magnetic beads in the groove 1025, and after the uniformly mixed magnetic beads are rinsed in the rinsing cavity 6, the magnetic attraction module is started to adsorb the magnetic beads in the groove 1025;
fixing a central shaft, rotating the main box 3 clockwise to enable the other rinsing cavity 6 to be aligned with the groove 1025, starting the oscillating module to mix magnetic beads in the groove 1025 uniformly, and starting the magnetic attraction module to adsorb the magnetic beads in the groove 1025 after the mixed magnetic beads are rinsed for the second time in the rinsing cavity 6;
fixing a central shaft, rotating the main box 3 clockwise to enable the drying cavity 7 to be aligned with the groove 1025, and carrying out the next operation after the magnetic beads in the groove 1025 are dried;
after drying, fixing the shaft spindle, rotating the main box 3 clockwise to enable the eluting cavity 8 to be aligned with the groove 1025, starting the oscillating module to mix the magnetic beads in the groove 1025 uniformly, and starting the magnetic attraction module to adsorb the magnetic beads in the groove 1025 after the mixed magnetic beads are eluted in the eluting cavity 8;
fixing a central shaft, rotating the main box 3 clockwise to enable the eluting cavity 8 to be communicated with a suction hole 1024 of a central runner, and pulling the piston rod upwards to suck the eluent in the eluting cavity 8 into the cavity of the shaft body 101;
the central shaft is fixed, the main box 3 is rotated clockwise, one of the liquid dividing channels is communicated with the suction hole 1024 of the central channel, the piston rod is pushed downwards to inject the eluent in the cavity of the shaft body 101 into the detection tubes 13 through the liquid dividing channels (the injection amount is preferably 10-15 microliters), four liquid dividing channels are arranged in the embodiment, and the steps are repeated, so that the eluent is sequentially injected into each detection tube 13;
the central shaft is fixed, the main box 3 is rotated anticlockwise, the drying cavity 7 is communicated with the suction hole 1024 of the central runner, and the piston rod is pushed downwards to inject redundant eluent in the cavity of the shaft body 101 into the drying cavity 7;
fixing the central shaft, rotating the main box 3 clockwise, enabling the paraffin oil cavity 16 to be communicated with the suction hole 1024 of the central runner, and pulling the piston rod upwards to suck the paraffin oil into the cavity of the shaft body 101;
fixing a central shaft, rotating the main box 3 clockwise, enabling the liquid separation channel 9 to be communicated with the suction hole 1024 of the central flow channel, pushing the piston rod downwards to inject paraffin oil in the cavity of the shaft body 101 into the detection pipes 13, repeating the steps, and sequentially injecting the paraffin oil into each detection pipe 13;
the central shaft is fixed, the main box 3 is rotated clockwise, the cracking cavity 4 is communicated with the suction hole 1024 of the central runner, and then PCR detection analysis is started.
Finally, it is noted that the above embodiments are only for illustrating the technical solution of the present utility model and not for limiting the same, and although the present utility model has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications and equivalents may be made thereto without departing from the spirit and scope of the technical solution of the present utility model, which is intended to be covered by the scope of the claims of the present utility model.
Claims (9)
1. A nucleic acid detection assay kit comprising:
the central shaft is provided with a central flow passage, a suction module (2) is arranged in the central flow passage, and a groove (1025) is also formed in the central shaft;
the main box (3) is sequentially distributed with a cracking cavity (4), a combining cavity (5), a rinsing cavity (6), a drying cavity (7), an eluting cavity (8) and a liquid separating channel (9) around the circumference direction of the central shaft, the main box (3) is sleeved outside the central shaft and can rotate around the central shaft, so that one of the liquid separating channel (9) and each cavity is communicated with the central flow channel, the top of each cavity is sealed in an initial state, and the main box (3) is in sealed connection with the central shaft;
and a base (10) configured with a magnetic attraction module for receiving magnetic beads into the groove (1025).
2. The nucleic acid detecting and analyzing kit according to claim 1, wherein a vertically penetrating mounting hole is provided in the center of the main case (3), and a platform (301) is provided at the bottom of the main case (3);
the central shaft includes:
a hollow cylindrical shaft body (101), the shaft body (101) penetrating through the mounting hole;
and the pressure plate (102) is fixedly arranged at the bottom of the shaft body (101), the top end face of the pressure plate (102) is attached to the bottom end face of the platform (301) and is provided with a sealing gasket (11) in a pressing mode, the pressure plate (102) is provided with a radial channel (1021) communicated with the inner cavity of the shaft body (101), the other end of the radial channel (1021) is alternatively communicated with the liquid separation channel (9) and various cavities, and the top end of the pressure plate (102) is provided with a vertically-through air vent (1022).
3. The nucleic acid detecting and analyzing kit according to claim 2, wherein a drainage column (12) for clamping a detecting tube (13) is fixedly arranged at the bottom of the main box (3), and the liquid separating channel (9) comprises:
a reaction liquid flow passage (901), one end of which can be communicated with the central flow passage, and the other end of which is communicated with the drainage column (12);
and a ventilation passage (902) having one end in communication with the drainage column (12), and when one end of the reaction liquid flow passage (901) is in communication with the center flow passage, the other end of the ventilation passage (902) is in communication with the ventilation hole (1022).
4. A nucleic acid detecting and analyzing kit according to claim 3, wherein the main box (3) has a sinking table (302), the liquid dividing channels (9) are provided in plural and are all provided on the sinking table (302), and the top of each liquid dividing channel (9) is opened and sealed by a coating film.
5. A nucleic acid detecting and analyzing kit according to any one of claims 2 to 4, wherein the radial passage (1021) is provided on a bottom end surface of the platen (102), and further comprising a center pressing piece (14) which is fixedly provided on the bottom end surface of the platen (102) and is configured to seal the radial passage (1021).
6. The nucleic acid detecting and analyzing kit according to any one of claims 2 to 4, wherein the platen (102) is provided with a clamping groove (1023), the base (10) is cylindrical and sleeved outside the main case (3), and the base (10) is fixedly provided with a barrier strip clamped in the clamping groove (1023).
7. A nucleic acid detecting and analyzing kit according to any one of claims 2 to 4, wherein the top end of the shaft body (101) extends out of the main case (3) and is provided with a clamping hole, and the kit further comprises a gland (15) provided with a buckle capable of being clamped into the clamping hole.
8. A nucleic acid detecting and analyzing kit according to any one of claims 1 to 4, wherein the main casing (3) is further provided with a vent (303), and the central flow passage is in communication with the vent (303) in an initial state.
9. A nucleic acid detection assay kit according to any one of claims 1 to 4, further comprising a paraffin oil cavity (16) in communication with the central flow channel, the paraffin oil cavity (16) being on the same circumference as the elution cavity (8) and being located on a side of the elution cavity (8) facing away from the drying cavity (7).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320934203.2U CN219490025U (en) | 2023-04-23 | 2023-04-23 | Nucleic acid detection and analysis kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320934203.2U CN219490025U (en) | 2023-04-23 | 2023-04-23 | Nucleic acid detection and analysis kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219490025U true CN219490025U (en) | 2023-08-08 |
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ID=87506068
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320934203.2U Active CN219490025U (en) | 2023-04-23 | 2023-04-23 | Nucleic acid detection and analysis kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219490025U (en) |
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2023
- 2023-04-23 CN CN202320934203.2U patent/CN219490025U/en active Active
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