CN113278517A - Portable integrated POCT nucleic acid rapid detector - Google Patents
Portable integrated POCT nucleic acid rapid detector Download PDFInfo
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- CN113278517A CN113278517A CN202110647754.6A CN202110647754A CN113278517A CN 113278517 A CN113278517 A CN 113278517A CN 202110647754 A CN202110647754 A CN 202110647754A CN 113278517 A CN113278517 A CN 113278517A
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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Abstract
The invention discloses a portable integrated POCT nucleic acid rapid detector, which comprises: a base; the central column is provided with a first accommodating chamber, one end of the central column is fixed on the base, and the other end of the central column can be selectively opened or closed; the turntable is rotatably sleeved on the central column; the 4 reagent releasing devices are arranged on the central column in a movable way at intervals and are used for releasing the reagent; the 8 chip clamping grooves are arranged on the turntable at intervals and are selectively communicated with the 4 reagent releasing devices; the chip card slot includes: the first shell is provided with a groove for placing the microfluidic chip; the heating aluminum block is arranged at the bottom of the groove and used for heating the microfluidic chip; the waste liquid cavity micro pump interface is communicated with a waste liquid cavity of the micro-fluidic chip and is used for maintaining negative pressure on the waste liquid cavity of the micro-fluidic chip; the detection cavity micro pump interface is communicated with the detection cavity of the micro-fluidic chip and is used for maintaining negative pressure for the detection cavity of the micro-fluidic chip. The invention has the characteristics of short detection time and simple operation.
Description
Technical Field
The invention relates to the technical field of medical equipment, in particular to a portable integrated POCT nucleic acid rapid detector.
Background
The virus nucleic acid detection plays an important role in the investigation of infection sources, but at present, because nucleic acid extraction and detection instruments are large in size and complex to operate, samples collected on epidemic sites need to be transferred to large laboratories through long-time cold chain transportation, and in the long-time transportation process, the samples have the risks of high-temperature exposure, cross infection and sample contamination, and the transportation time of patients is prolonged.
At present, nucleic acid detection is divided into two parts, namely nucleic acid extraction and nucleic acid detection, a large laboratory is relied on, a large precision instrument is required to be independently operated, extracted nucleic acid needs to be transferred to a detection instrument for detection, time and labor are consumed, an operator is extremely relied on, long waiting time is required, and quick detection of epidemic situations on site cannot be realized.
In order to ensure the efficiency of nucleic acid detection expansion, meet the requirements of quickly and simply obtaining a result, simplify the process and reduce the volume of an instrument, conventional nucleic acid amplification methods such as PCR (repeated temperature rise and fall, which requires a large-scale centrifuge), ASBA (high requirements for samples), RCA (long reaction time), HAD (high requirements for operation), RPA (high requirements for operators) and the like cannot be realized.
In order to realize the whole nucleic acid detection process between the sizes of the squares on the epidemic situation site, the conventional nucleic acid extraction method comprises the following steps: phenol-chloroform extraction (requiring toxic reagents), magnetic particle extraction (requiring magnetic field construction), salting-out (incomplete removal of impurities) and glass bead extraction (residual reagents) do not meet the requirement for rapid extraction on site, and have high requirements for instruments.
And the detection equipment of the like products in the market is expensive, the operation is complex, the detection time is long, the basic medical mechanism is difficult to popularize, and meanwhile, the existing products have the problems of low detection sensitivity, complex interpretation, missed detection, wrong detection, delayed treatment and the like.
Disclosure of Invention
The invention aims to design and develop a portable integrated POCT nucleic acid rapid detector, which can detect the detection of various pathogen nucleic acids by matching a plurality of reagent releasing devices with a plurality of chip card grooves and combining a matched detection reagent.
The technical scheme provided by the invention is as follows:
a portable integrated POCT nucleic acid rapid detector comprises:
a base; and
the central column is provided with a first accommodating chamber, one end of the central column is fixed on the base, and the other end of the central column can be selectively opened or closed;
the turntable is rotatably sleeved on the central column;
a plurality of reagent releasing devices which are arranged on the central column at intervals and used for releasing the reagent;
a plurality of chip card slots which are arranged on the turntable at intervals and can be selectively communicated with the plurality of reagent releasing devices;
wherein, the chip card slot includes:
the first shell is provided with a groove and used for placing the microfluidic chip;
the heating aluminum block is arranged at the bottom of the groove and used for heating the microfluidic chip;
the waste liquid cavity micro-pump interface is communicated with the waste liquid cavity of the micro-fluidic chip and is used for maintaining negative pressure on the waste liquid cavity of the micro-fluidic chip;
and the detection cavity micropump interface is communicated with the detection cavity of the microfluidic chip and is used for maintaining negative pressure for the detection cavity of the microfluidic chip.
Preferably, the method further comprises the following steps:
the second shell is sleeved on the outer side of the base, and a second accommodating chamber is formed between the second shell and the base;
the central column, the rotary table, the plurality of reagent releasing devices and the plurality of chip clamping grooves are all arranged in the second accommodating chamber.
Preferably, the first accommodating chamber includes therein:
the vacuum pump is communicated with the waste liquid cavity micro-pump interface and the detection cavity micro-pump interface;
a plurality of kits connected to the plurality of reagent releasing devices.
Preferably, the reagent delivery device comprises:
the sliding seat is arranged on the side surface of the central column and can move longitudinally along the central column;
the slow table is arranged on the sliding seat and can move back and forth;
and the reagent releasing channels are respectively connected with the reagent boxes in a one-to-one correspondence manner, one ends of the reagent releasing channels are fixed on the slow table, and the other ends of the reagent releasing channels are respectively communicated with the reagent injecting holes of the microfluidic chip in a one-to-one correspondence manner.
Preferably, the sliding seat includes:
the bracket is fixed on the side surface of the central column;
a first stepping motor provided on the bracket;
the screw rod is rotatably arranged in the bracket and is connected with the output end of the first stepping motor;
and the sliding block is arranged on the lead screw in a sliding manner.
Preferably, the method further comprises the following steps:
a peristaltic pump disposed in the slide;
wherein the plurality of agent release channels surround the peristaltic pump for controlling the dosage of agent release.
Preferably, the first housing further includes:
the first slide way is arranged in parallel with the groove and is provided with a through hole;
wherein the through hole is communicated with the groove.
Preferably, the chip card slot further includes:
the second slideway is communicated with the groove;
one end of the touch switch is arranged in the groove, and the touch switch is slidably arranged in the second slide way and used for judging whether the microfluidic chip is inserted or not;
the clamping spring comprises a fixed end, a movable end and a clamping end;
the fixed end is fixed in the first slide way, the movable end is arranged in the first slide way in a sliding manner, and the clamping end penetrates through the through hole and is used for fixing the microfluidic chip;
and the ultraviolet lamp is arranged on the outer side of the detection cavity micropump interface.
Preferably, the method further comprises the following steps:
a temperature sensor disposed inside the heating aluminum block;
a heat insulating material disposed between the heating aluminum block and the first case;
and the second stepping motor is connected with the rotary disc.
Preferably, the second housing includes:
the touch screen control panel is connected with the temperature sensor, the first stepping motor and the second stepping motor and is used for displaying or adjusting parameters;
an observation window disposed on an upper portion of the microfluidic chip;
the feeding port is parallel to the chip card groove and is used for taking or putting the microfluidic chip;
the first switch is connected with the first stepping motor, the second stepping motor, the vacuum pump, the peristaltic pump, the touch screen control panel and the temperature sensor and is used for switching on and off a power supply;
the second switch is connected with the feeding port and used for opening the feeding port;
the third switch is connected with the feeding port and is used for closing the feeding port;
and the starting switch is connected with the vacuum pump and the peristaltic pump and is used for switching on or off the vacuum pump and the peristaltic pump.
The invention has the following beneficial effects:
(1) the portable integrated POCT nucleic acid rapid detector designed and developed by the invention integrates nucleic acid extraction and nucleic acid detection, realizes the whole process of one-time sample addition and one-step extraction and detection, avoids the influence of the external environment to the maximum extent, solves the problems of dependence on large laboratories and operators and the like, further simplifies the instrument to the utmost extent, has small volume, simple operation and low price, can simultaneously detect a plurality of chips, automatically add samples, automatically detect and directly output results.
(2) The portable integrated POCT nucleic acid rapid detector designed and developed by the invention can automatically output results in a short time after a patient sample is put into the detector on site, does not need any operation or professional operators, is not influenced by external environment, realizes on-site sampling and on-site result output, and can be popularized and popularized in primary medical institutions.
(3) The portable integrated POCT nucleic acid rapid detector designed and developed by the invention has stronger sensitivity and specificity, the detection result can be observed by naked eyes, and the matched detection reagent can be combined to detect the nucleic acid detection of various pathogens, such as neocoronaria, SARS, hepatitis B, hantavirus and the like.
Drawings
FIG. 1 is a schematic diagram of the internal front view structure of the portable integrated POCT nucleic acid rapid detector of the present invention.
FIG. 2 is a schematic view of the internal overall structure of the portable integrated POCT nucleic acid rapid detector of the present invention.
FIG. 3 is a schematic diagram of the internal top view structure of the portable integrated POCT nucleic acid rapid detector of the present invention.
Fig. 4 is a schematic top view of a chip card slot according to the present invention.
Fig. 5 is a schematic view of an overall structure of the chip card slot according to the present invention.
Fig. 6 is a schematic structural diagram of the microfluidic chip according to the present invention.
Fig. 7 is an exploded view of the microfluidic chip according to the present invention.
Fig. 8 is a schematic structural diagram of a first main layer of the chip according to the present invention.
Fig. 9 is a schematic bottom view of the second main layer of the chip according to the present invention.
FIG. 10 is a schematic front view of the integrated POCT nucleic acid rapid detector of the present invention.
FIG. 11 is a schematic diagram of a side view of the portable integrated POCT nucleic acid rapid detector of the present invention.
FIG. 12 is a schematic top view of the integrated POCT nucleic acid rapid detector of the present invention.
Detailed Description
The present invention is described in further detail below in order to enable those skilled in the art to practice the invention with reference to the description.
As shown in fig. 1, fig. 2 and fig. 3, the portable integrated POCT nucleic acid rapid detector provided by the present invention specifically comprises: the device comprises a central column 120, a base 130, a turntable 140, a plurality of reagent releasing devices 150 and a plurality of chip clamping grooves 160, wherein one end of the central column 120 is fixed on the base 130, the other end of the central column can be selectively opened or closed, and the central axis of the central column 120 coincides with the central axis of the base 130; in this embodiment, the other end of the central column 120 is provided with a cover plate which can be opened and closed in a hinged manner, and the central column 120 is provided with a first accommodating chamber; the turntable 140 is rotatably sleeved on the central column 120 through a second stepping motor, and the turntable 140 is rotatably arranged on the base 130; a plurality of reagent releasing devices 150 are arranged on the central column 120 at intervals for releasing the reagent; a plurality of chip card slots 160 are spaced on the turntable 140, and the plurality of chip card slots 160 are selectively communicated with the plurality of reagent releasing devices 150, and microfluidic chips 170 can be inserted into the plurality of chip card slots 160 for extraction and detection of nucleic acids.
The first receiving chamber is provided therein with a vacuum pump for maintaining a negative pressure inside the microfluidic chip 170 and a plurality of reagent cartridges (not shown) connected to the plurality of reagent releasing devices 150.
In this embodiment, 8 chip card slots 160 are equidistantly arranged on the turntable 140, 4 reagent releasing devices 150 are equidistantly arranged on the center column 120, the second stepping motor serves as a driving device to drive the turntable 140 to rotate, and the purpose that the reagent releasing devices 150 can release reagents to the microfluidic chip 170 is achieved by differentiating the separation angle between each chip card slot 160 and controlling the stroke of the second stepping motor.
In this embodiment, the number of the reagent cartridges is 4, and the reagent cartridges are detachably disposed on the upper portion of the first accommodating chamber, so that the reagent cartridges can be replaced after the central column 120 is opened.
As shown in fig. 1 and 2, the reagent releasing device 150 includes: a slide block, a slow station 155, and a plurality of reagent release channels 156, wherein the slide block comprises: the support 152 is fixed on the side surface of the central column 120, and the support 152 comprises an upper support frame, a lower support frame and a support wall, wherein the support wall is arranged between the upper support frame and the lower support frame, and is fixed on the side surface of the central column 120; the first stepping motor 151 is fixed on the upper support frame, and the output end of the first stepping motor 151 penetrates through the upper support frame; the screw 153 is parallel to the support wall and is rotatably disposed between the upper support frame and the lower support frame, and the screw 153 is connected to the output end of the first stepper motor 151; the sliding block 154 is slidably arranged on the lead screw 153; the slow stage 155 is arranged on the slide block 154, and the slow stage 155 can move back and forth; in this embodiment, an elastic device is disposed between the slow stage 155 and the sliding block 154, and the elastic device is connected to the first stepping motor 151 and can move laterally under the driving of the first stepping motor 151; the plurality of reagent release channels 156 are respectively connected with the plurality of reagent boxes in a one-to-one correspondence manner, one end of each reagent release channel 156 is fixed on the slow stage 155, and the other end of each reagent release channel 156 is respectively communicated with the reagent injection hole of the microfluidic chip 170 in a one-to-one correspondence manner; still be provided with the peristaltic pump in the slider 154, a plurality of reagent release channels 156 surround on the peristaltic pump, adopt the drive method of peristaltic pump to realize continuous accurate application of sample operation, the peristaltic pump comprises drive arrangement, pump head and hose, steerable rotational speed and flow, stability and repeatability are than higher and difficult the production pollution.
In this embodiment, the number of the reagent releasing channels 156 is 4, and the reagent releasing channels are respectively communicated with the 4 reagent cartridges one by one.
As shown in fig. 6, the microfluidic chip 170 includes a chip top layer 210, a chip first body layer 220, a chip second body layer 230, and a chip bottom layer 240, which are sequentially stacked from top to bottom, and as shown in fig. 7, the chip top layer 210 includes a first reagent injection hole 211, a second reagent injection hole 212, a third reagent injection hole 213, a fourth reagent injection hole 214, and a fifth reagent injection hole 215.
As shown in fig. 8, the first body layer 220 of the chip includes a lysis chamber 224, a first washing chamber 222, a second washing chamber 221, a first elution chamber 223, a communication chamber 226, a micro-control flow area 227, a detection chamber 225 and a plurality of reaction micro-chambers 228, the lysis chamber 224 is communicated with the first reagent injection hole 211, the first washing chamber 222 is communicated with the second reagent injection hole 212, the second washing chamber 221 is communicated with the third reagent injection hole 213, the first elution chamber 223 is communicated with the fourth reagent injection hole 214, the communication chamber 226 is disposed at the center of the lysis chamber 224, the first washing chamber 222, the second washing chamber 221 and the first elution chamber 223, the communication chamber 226 is communicated with the lysis chamber 224, the first washing chamber 222 and the second washing chamber 221, and one end of the micro-control flow area 227 is communicated with the communication chamber 226, the other end of the micro-controlled flow area 227 is communicated with the detection cavity 225, the detection cavity 225 is communicated with the fifth reagent injection hole 215, and the detection cavity 225 is communicated with the reaction microchambers 228 in a one-to-one correspondence manner through a plurality of mixing channels which are arranged at equal intervals and used for detecting nucleic acid by a plurality of reaction reagents.
In this embodiment, the number of the reaction micro-chambers 228 is 6.
As shown in fig. 9, the chip second body layer 230 includes: draw room 231, second elution chamber, waste liquid chamber 233, first gas pocket 232 and second gas pocket 234, it has inward flange for through-hole structure and one end to draw room 231, second elution chamber with first elution chamber 223 is linked together, waste liquid chamber 233 is groove structure, just second elution chamber pass through the recess with the one end of drawing room 231 is linked together, the other end (flange upper portion) of drawing room 231 is used for placing the solid phase and draws the membrane, draw room 231 with it is linked together to converge the chamber 226, the diameter of converging the chamber 226 with the internal diameter of flange is the same, waste liquid chamber 233 one end with the one end of drawing room 231 is linked together, and the other end is linked together with second gas pocket 234, waste liquid chamber 233 middle berth is equipped with water absorbing material, first gas pocket 232 with it is linked together to detect the chamber 225.
In this embodiment, the first reagent injection hole 211, the second reagent injection hole 212, the third reagent injection hole 213, and the fifth reagent injection hole 215 are respectively connected to the 4 reagent release channels 156 in a one-to-one correspondence manner, and the cell lysis solution is added to the fourth reagent injection hole 214 at the same time when the nucleic acid is added to the microfluidic chip, so that the reagent release channels 156 are not needed.
As shown in fig. 4 and 5, the chip card slot 160 includes: the micro-fluidic chip detection device comprises a first shell 160, a heating aluminum block 161, a waste liquid cavity micro-pump interface 165, a detection cavity micro-pump interface 166, an ultraviolet lamp 167, a first slide rail 163, a second slide rail 168, a touch switch 162 and a clamping spring 164, wherein a groove, the first slide rail 163 and the second slide rail 168 are arranged on the first shell 160, and the groove is used for placing a micro-fluidic chip 170; the first slide rail 163 is arranged in parallel with the groove, and a through hole is formed in the first slide rail 163 and communicated with the groove; the heating aluminum block 161 is arranged at the bottom of the groove and used for heating the micro-fluidic chip 170, and temperature compensation correction of the internal temperature of the micro-fluidic chip 170 and the internal measured temperature of the heating aluminum block 161 is completed through a temperature control experiment; a heating resistance film is attached to the lower part of the heating aluminum block 161, the interior of the microfluidic chip 170 is heated through heat conduction of the heating aluminum block 161, and in order to ensure the heating effect, a heat insulation material is padded below the heating resistance film, so that invalid dissipation of heat is reduced as much as possible, and meanwhile, the accuracy of a temperature measurement result is ensured; the waste liquid cavity micro pump interface 165 is communicated with the waste liquid cavity 233 of the micro fluidic chip 170 through a second air hole 234, and is used for maintaining negative pressure on the waste liquid cavity 233 of the micro fluidic chip 170; the detection cavity micro pump interface 166 is communicated with the detection cavity 225 of the microfluidic chip 170 and is used for maintaining negative pressure to the detection cavity 225 of the microfluidic chip 170, and the waste liquid cavity micro pump interface 165 and the detection cavity micro pump interface 166 are both connected with a vacuum pump; a second slideway 168 communicates with the recess; one end of the touch switch 162 is disposed in the groove, and the touch switch 162 is slidably disposed in the second slide 168 for determining whether the microfluidic chip 170 is inserted; the clamping spring 164 comprises a fixed end, a movable end and a clamping end, wherein the fixed end is fixed in the first slide rail 163, the movable end is slidably arranged in the first slide rail 163, and the clamping end penetrates through the through hole and is used for fixing the microfluidic chip 170; the ultraviolet lamp 167 is arranged outside the detection cavity micro-pump interface 166, and the detection result is more convenient to observe under the ultraviolet lamp.
The heating aluminum block 161 is provided with a temperature sensor for measuring the temperature in the microfluidic chip 170.
As shown in fig. 10, 11 and 12, a second casing 110 is sleeved outside the base 130, and a second accommodating chamber is formed between the second casing 110 and the base 130; the central column 120, the rotating disc 140, the reagent releasing devices 150, and the chip slots 160 are all disposed in the second accommodating chamber, one end of the central column 120 is disposed inside the second accommodating chamber, and the other end of the central column passes through the second housing 110, so as to facilitate opening of the central column 120.
The second shell is provided with: the temperature sensor is connected with the touch screen control panel 111, the first stepping motor 151 and the second stepping motor, and the touch screen control panel 111 is used for displaying temperature, adjusting output power of the first stepping motor 151 or the second stepping motor, and controlling the second stepping motor to be started or closed; the observation window 113 is arranged on the upper part of the microfluidic chip 170; the feeding port 114 is parallel to the chip clamping groove 160 and is used for taking or putting the microfluidic chip 170; the first switch 115 is connected with the first stepping motor 151, the second stepping motor, the vacuum pump, the peristaltic pump, the touch screen control panel 111 and the temperature sensor, and is used for switching on and off a power supply; the second switch 116 is connected with the feeding port 114 and is used for opening the feeding port 114; a third switch 117 connected to the feeding port 114 for closing the feeding port 114; the start switch 118 is connected to the vacuum pump and the peristaltic pump, and is used for turning on or off the vacuum pump and the peristaltic pump.
In this embodiment, the feed port 114 is a hinged, reversible cover plate.
The portable integrated POCT nucleic acid rapid detector provided by the invention has the use process that:
connecting the detector with a power supply, pressing down a first switch 115, then pressing down a second switch 116, smoothly inserting the microfluidic chip 170 filled with nucleic acid and cell lysate along the chip card slot 160 to the touch switch 162, wherein the touch screen control panel 111 prompts that the microfluidic chip 170 is inserted, after the microfluidic chip 170 is completely inserted, the clamping spring 164 forms a pressing force on the microfluidic chip 170, so that the position deviation can be prevented during the diagnosis process after the microfluidic chip 170 is inserted into the chip card slot 160, the important effect on the stability and safety of the microfluidic chip 170 in the use process is achieved, then pressing down the third switch 117, starting a second step motor through the touch screen control panel 111, rotating the turntable 140 through a mechanical positioning design until the accurate position relation between the chip card slot 160 and the reagent release device 150 in the vertical direction is corresponding, starting a first stepping motor 151 and a heating aluminum block 161 through a touch screen control panel 111, adjusting 3 reagent release channels 156 to correspond to a first reagent injection hole 211, a second reagent injection hole 212 and a third reagent injection hole 213 respectively while keeping the micro-fluidic chip 170 at a constant temperature, maintaining negative pressure in the micro-fluidic chip 170 through a waste liquid cavity micro-pump interface 165, injecting WASH-A and WASH-B into the first reagent injection hole 211 and the second reagent injection hole 212 respectively, extracting nucleic acid through a solid phase extraction membrane, maintaining negative pressure in the micro-fluidic chip 170 through a detection cavity micro-pump interface 166 after completion, injecting eluent into the third reagent injection hole 213, connecting a 4 th reagent release channel 156 with a fifth reagent injection hole 215 correspondingly through movement of a buffer 155, injecting a primer reagent into the fifth reagent injection hole 215 to detect a nucleic acid template in a detection cavity 225, and finally, the mixed solution flows to the corresponding 6 reaction micro chambers 228 from the detection cavity 225 through the 6 mixing channels on the periphery, the heating temperature of the heating aluminum block 161 is controlled through the touch screen control panel 111, the reaction of nucleic acid detection is carried out at the constant temperature of 60-65 ℃, the reaction is finished when specific color change occurs, the reaction process and the result are observed through the observation window 113, the second switch 116 is pressed after the detection is finished, the micro-fluidic chip 170 is taken out for treatment, the third switch 117 is pressed, and the instrument is closed.
According to the portable integrated POCT nucleic acid rapid detector designed and developed by the invention, through the mode that 4 reagent releasing devices are matched with 8 chip clamping grooves, the cost of the detector is saved, 8 microfluidic chips can be simultaneously detected, on-site sampling and on-site detection are carried out, results are automatically obtained within thirty minutes, no operation is needed, the sensitivity is high, the specificity is strong, the detection results are easy to observe, the results are accurate, and the nucleic acid detection can be carried out on various pathogens.
While embodiments of the invention have been described above, it is not limited to the applications set out in the description and the embodiments, which are fully applicable in all kinds of fields suitable for the invention, and further modifications may readily be effected by those skilled in the art, without departing from the general concept defined by the claims and the equivalents thereof, and the invention is therefore not limited to the specific details and embodiments shown and described herein.
Claims (10)
1. A portable integrated POCT nucleic acid rapid detector is characterized by comprising:
a base; and
the central column is provided with a first accommodating chamber, one end of the central column is fixed on the base, and the other end of the central column can be selectively opened or closed;
the turntable is rotatably sleeved on the central column;
a plurality of reagent releasing devices which are arranged on the central column at intervals and used for releasing the reagent;
a plurality of chip card slots which are arranged on the turntable at intervals and are selectively communicated with the plurality of reagent releasing devices;
wherein, the chip card slot includes:
the first shell is provided with a groove and used for placing the microfluidic chip;
the heating aluminum block is arranged at the bottom of the groove and used for heating the microfluidic chip;
the waste liquid cavity micro-pump interface is communicated with the waste liquid cavity of the micro-fluidic chip and is used for maintaining negative pressure on the waste liquid cavity of the micro-fluidic chip;
and the detection cavity micro pump interface is communicated with the detection cavity of the micro-fluidic chip and is used for maintaining negative pressure for the detection cavity of the micro-fluidic chip.
2. The portable integrated POCT nucleic acid rapid detector as claimed in claim 1, further comprising:
the second shell is sleeved on the outer side of the base, and a second accommodating chamber is formed between the second shell and the base;
the central column, the rotary table, the plurality of reagent releasing devices and the plurality of chip clamping grooves are all arranged in the second accommodating chamber.
3. The portable integrated POCT nucleic acid rapid detector as claimed in claim 2, wherein the first containing chamber comprises:
the vacuum pump is communicated with the waste liquid cavity micro-pump interface and the detection cavity micro-pump interface;
a plurality of kits connected to the plurality of reagent releasing devices.
4. The portable integrated POCT nucleic acid rapid detector of claim 3, wherein the reagent releasing device comprises:
the sliding seat is arranged on the side surface of the central column and can move longitudinally along the central column;
the slow table is arranged on the sliding seat and can move back and forth;
and the reagent releasing channels are respectively connected with the reagent boxes in a one-to-one correspondence manner, one ends of the reagent releasing channels are fixed on the slow table, and the other ends of the reagent releasing channels are respectively communicated with the reagent injecting holes of the microfluidic chip in a one-to-one correspondence manner.
5. The portable integrated POCT nucleic acid rapid detector as claimed in claim 4, wherein the slide base comprises:
the bracket is fixed on the side surface of the central column;
a first stepping motor provided on the bracket;
the screw rod is rotatably arranged in the bracket and is connected with the output end of the first stepping motor;
and the sliding block is arranged on the lead screw in a sliding manner.
6. The portable integrated POCT nucleic acid rapid detector as claimed in claim 5, further comprising:
a peristaltic pump disposed in the slide;
wherein the plurality of agent release channels surround the peristaltic pump for controlling the dosage of agent release.
7. The portable integrated POCT nucleic acid rapid detector of claim 6, wherein the first housing further comprises:
the first slide way is arranged in parallel with the groove and is provided with a through hole;
wherein the through hole is communicated with the groove.
8. The portable integrated POCT nucleic acid rapid detector of claim 7, wherein the chip card slot further comprises:
the second slideway is communicated with the groove;
one end of the touch switch is arranged in the groove, and the touch switch is slidably arranged in the second slide way and used for judging whether the microfluidic chip is inserted or not;
the clamping spring comprises a fixed end, a movable end and a clamping end;
the fixed end is fixed in the first slide way, the movable end is arranged in the first slide way in a sliding manner, and the clamping end penetrates through the through hole and is used for fixing the microfluidic chip;
and the ultraviolet lamp is arranged on the outer side of the detection cavity micropump interface.
9. The portable integrated POCT nucleic acid rapid detector as claimed in claim 8, further comprising:
a temperature sensor disposed inside the heating aluminum block;
a heat insulating material disposed between the heating aluminum block and the first case;
and the second stepping motor is connected with the rotary disc.
10. The portable integrated POCT nucleic acid rapid detector as claimed in claim 9, wherein the second housing comprises:
the touch screen control panel is connected with the temperature sensor, the first stepping motor and the second stepping motor and is used for displaying or adjusting parameters;
an observation window disposed on an upper portion of the microfluidic chip;
the feeding port is parallel to the chip card groove and is used for taking or putting the microfluidic chip;
the first switch is connected with the first stepping motor, the second stepping motor, the vacuum pump, the peristaltic pump, the touch screen control panel and the temperature sensor and is used for switching on and off a power supply;
the second switch is connected with the feeding port and used for opening the feeding port;
the third switch is connected with the feeding port and is used for closing the feeding port;
and the starting switch is connected with the vacuum pump and the peristaltic pump and is used for switching on or off the vacuum pump and the peristaltic pump.
Priority Applications (1)
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| CN114134027A (en) * | 2021-12-01 | 2022-03-04 | 马晟利 | Portable fast detector for new coronavirus |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114134027A (en) * | 2021-12-01 | 2022-03-04 | 马晟利 | Portable fast detector for new coronavirus |
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