CN215050301U

CN215050301U - Nucleic acid detection analyzer

Info

Publication number: CN215050301U
Application number: CN202022469292.7U
Authority: CN
Inventors: 汪云飞; 潘能科; 任鹏鹏
Original assignee: Zhongyuan Huiji Biotechnology Co Ltd
Current assignee: Zhongyuan Huiji Biotechnology Co Ltd
Priority date: 2020-10-30
Filing date: 2020-10-30
Publication date: 2021-12-07
Anticipated expiration: 2030-10-30

Abstract

The invention relates to nucleic acid detection, in particular to a nucleic acid detection analyzer, which comprises a rack, a temperature control device, a fluorescence detection device, a thermal cover device and a reagent tube, wherein the temperature control device, the fluorescence detection device, the thermal cover device and the reagent tube are arranged on the rack; the frame comprises a sample adding table, and an insertion hole is formed in the sample adding table; the temperature control device and the fluorescence detection device are arranged between the bottom plate and the sample adding platform; the hot cover device is arranged on the frame; the reagent tube is arranged on the insertion hole and is covered by the thermal cover device; the position of the reagent tube is matched with the position of the temperature control device; the detection position of the fluorescence detection device is opposite to the reagent tube. The invention has the advantages of multiple scenes, low operation requirement and low price and is suitable for popularization.

Description

Nucleic acid detection analyzer

Technical Field

The invention relates to nucleic acid detection, in particular to a nucleic acid detection analyzer.

Background

The nucleic acid detection technology is a molecular biological detection method, and comprises the steps of extracting nucleic acid fragments (DNA or RNA) from a sample, then carrying out replication and amplification on the nucleic acid fragments through polymerase chain reaction, and finally detecting the nucleic acid, thereby determining the specific category or source of the nucleic acid in the sample.

The nucleic acid detection method is simple and easy to implement, has extremely wide application, and can obtain an accurate result only by a small amount of samples. During PCR detection, a sample is firstly sampled, and is usually collected into a sampling tube by using a swab, and then is extracted from the sampling tube into a kit, and finally is placed into an instrument for reaction. In the process of nucleic acid extraction and amplification, reagents such as a first reagent, a second reagent and the like need to be added step by step to be mixed with a sample.

In the existing nucleic acid detection scheme, two instruments are generally used, a nucleic acid extraction process is firstly carried out by using a nucleic acid extractor, the extracted nucleic acid is manually transferred into a kit, and the kit is installed into a corresponding nucleic acid detection analyzer (such as a QPCR (quantitative polymerase chain reaction) instrument) for detection. This solution uses a lot of equipment, has many manual steps, requires professional operators, and needs to be performed in a specific environment to prevent cross contamination. The whole detection process time is longer.

At present, the nucleic acid extraction workstation connects the nucleic acid extraction equipment and the nucleic acid detection equipment through a mechanical wall to realize the transfer process of the nucleic acid, thereby realizing full automation. However, the nucleic acid extraction workstation has a complicated structure, large equipment volume and high price, and also needs professional operation.

With the increase of the demand of nucleic acid detection and the popularization and promotion of nucleic acid detection technology, it is urgently needed in the market to provide a nucleic acid detection analyzer with multiple application scenes, low operation requirements and a price suitable for promotion.

Disclosure of Invention

The invention provides a nucleic acid detection analyzer and a detection method thereof, which aim to solve the problems of complex operation, few application scenes, complex structure and high cost of the existing instrument.

In order to achieve the purpose, the invention adopts the following technical means:

a nucleic acid detection analyzer comprises a frame, a temperature control device, a fluorescence detection device, a thermal cover device and a reagent tube,

the sampling device comprises a bottom plate and a sampling platform arranged on the bottom plate, wherein the sampling platform is provided with an insertion hole;

the temperature control device and the fluorescence detection device are arranged between the bottom plate and the sample adding platform;

the hot cover device is arranged on the frame;

the reagent tube is arranged on the insertion hole and is covered by the thermal cover device;

the position of the reagent tube is matched with the position of the temperature control device (the matching refers to that one end of the reagent tube is positioned in the temperature control device after the reagent tube is installed, and the temperature control device can heat the reagent tube); the detection position of the fluorescence detection device is opposite to the reagent tube (the detection position mentioned here is opposite to the reagent tube, and the fluorescence detection device can detect the particles in the reagent tube); the detection position of the fluorescence detection device is opposite to the reagent tube.

The one end that the test tube was covered by the heat lid is located application of sample platform top, the other end is located the below of adding the appearance platform, fluorescence detection device is located the below of reagent pipe, temperature control device is including being used for the moving platform to the heating of reagent pipe lower extreme, moving platform is located and adds between appearance platform and the fluorescence detection device.

The working principle of the invention is as follows:

adding the sample to the reagent vessel to mix the sample with the first reagent, opening the thermal cover means, placing the reagent vessel in the insertion hole and covering the thermal cover means,

the hot-cover device is heated up,

the temperature control device heats and incubates the reaction part of the reagent tube, releases the nucleic acid in the sample into the reagent tube,

adding a second reagent in the reagent storage tube into the reagent tube to be mixed with the sample,

controlling the temperature of the reaction part of the reagent tube by using a temperature control device, so that the mixture of the sample reagent in the reagent tube reaches a first temperature, and the nucleic acid is melted at the first temperature;

controlling the temperature of the reaction part of the reagent tube by using a temperature control device to enable the mixture of the sample reagent in the reagent tube to reach a second temperature, and copying the nucleic acid at the second temperature;

controlling the temperature of the reaction site of the reagent tube with a temperature control device such that the temperature of the mixture of the sample reagents in the reagent tube is cycled between a first temperature and a second temperature until amplification of the nucleic acid is completed;

the fluorescence detection device optically detects the nucleic acid in the reagent tube.

The number of the temperature control devices, the number of the fluorescence detection devices, the number of the thermal cover devices and the number of the reagent tubes are at least two, and the temperature control devices, the fluorescence detection devices, the number of the thermal cover devices and the number of the reagent tubes are matched in a one-to-one correspondence mode.

The frame still includes the bottom plate and installs support frame, the shell on the bottom plate, add the appearance platform and install on the support frame, hot lid device is installed on adding the appearance platform.

The temperature control device comprises a driving component, a guide component and a first heating component; the first heating assembly comprises a moving platform, the moving platform is provided with more than three heating grooves for heating, the end part of the reagent tube is positioned in one heating groove, and the moving platform moves along the guide assembly under the driving of the driving assembly, so that the end part of the reagent tube is positioned in different heating grooves.

The driving assembly comprises a temperature control driving motor, a bearing seat, a lead screw and a lead screw nut, wherein the temperature control driving motor is arranged on the rack; the guide assembly comprises two guide rails arranged on the frame; the moving platform of the first heating assembly is arranged on a screw nut and is provided with a sliding block which is connected with a guide rail in a sliding fit manner.

The reagent tube comprises four heating grooves, a guide rail, a ceramic heating sheet and two heat conduction copper sheets, wherein the four heating grooves are arranged in parallel with the guide rail, each heating groove is internally provided with the ceramic heating sheet and the heat conduction copper sheet tightly abutted against the ceramic heating sheet, a gap for the reagent tube to pass through is formed between the two heat conduction copper sheets, and the heat conduction copper sheets are attached to the outer wall of the reagent tube;

the four temperatures in the heating tank comprise a first target temperature and a second target temperature for polymerase chain reaction, a first transition temperature and a second transition temperature for transition, and numerical values of the first transition temperature, the first target temperature, the second target temperature and the second transition temperature are sequentially reduced.

The mobile platform is a thermally insulating material; the mobile platform is formed by installing two mutually connected heat insulation plates; the temperature control driving motor is a stepping motor; the mobile platform is provided with a trigger piece, the rack is provided with an induction switch, and the position of the trigger piece is matched with that of the induction switch; or a trigger is installed on the machine frame, an inductive switch is installed on the mobile platform, and the position of the trigger is matched with that of the inductive switch (the matching refers to that the trigger and the inductive switch move relatively to enable the inductive switch to be controlled to be triggered).

The hot cover device comprises a cover shell and a second heating assembly arranged on the cover shell; the second heating assembly comprises a heat-conducting plate and a heating element in contact with the heat-conducting plate, the lower surface of the heat-conducting plate is provided with an annular boss protruding downwards, the shape of the annular boss is matched with the end part of the reagent tube (the matched shape mentioned here refers to the annular boss can surround the end part of the reagent tube, so that the heating space covers the end part of the reagent tube), and the inner wall of the annular boss and the lower surface of the heat-conducting plate surround a heating space for heating the end part of the reagent tube.

The hot cover device also comprises a guide structure arranged on the cover shell, wherein the guide structure comprises a guide plate, a guide shaft arranged on the lower surface of the guide plate and a first elastic part, and the first elastic part is positioned between the heat-conducting plate and the guide plate; the heat-conducting plate is provided with a guide hole, and the guide hole is connected with the guide shaft in a matching manner.

The second heating component is characterized in that the heat conducting plate is provided with a first ejector rod through hole, the guide plate is provided with a second ejector rod through hole and a waist-shaped hole, and the end part of the ejector rod structure penetrates through the first ejector rod through hole and the second ejector rod through hole and is located in the heating space.

The cover shell comprises an upper cover shell and a lower cover shell which is matched and connected with the upper cover shell, the upper cover shell and the lower cover shell are both of a hollow structure, the upper end of the upper cover shell is sealed, and a first mounting step and a second mounting step are arranged on the inner wall of the lower cover shell; the guide plate of the guide structure is arranged on the first step, and the heat conduction plate of the second heating assembly is positioned on the second step.

The hot lid device still includes the ejector pin structure, the ejector pin structure is including installing the ejector pin mounting bracket on the lid shell, installing ejector pin driving piece on the ejector pin mounting bracket, the ejector pin that slides and set up on the ejector pin mounting bracket and be connected with the cooperation of ejector pin driving piece, the driving piece can drive the tip of ejector pin gets into in the heating space.

The ejector rod driving piece is a linear motor, a linear bearing is installed on the ejector rod installation frame, and the ejector rod is installed on the linear bearing; a third groove is formed in the middle of the ejector rod and is connected with a sliding block of the linear motor in a matched mode; the end part of the ejector rod is provided with an ejector rod boss. (the ejector rod boss is used for puncturing a sealing film at a position corresponding to the reagent storage tube and is in fit connection with the first groove at the top of the reagent storage tube so as to ensure that the reagent storage tube is vertically stressed downwards and is pushed to move downwards.)

The reagent tube comprises a tube cover and a reaction tube, wherein the tube cover is provided with a sample adding hole and a reagent adding hole; a reagent storage tube is arranged in the reagent adding hole, a second reagent is arranged in the reagent storage tube, and a sealing film for sealing the sample adding hole and the reagent adding hole is arranged on the tube cover; the reaction tube is internally provided with a reaction cavity; the tube cover is matched and connected with the reaction tube to enable the reaction cavity to be in a sealed state.

The reagent storage tube is movably arranged in the reagent adding hole of the tube cover.

The reagent storage tube is tubular, one end of the reagent storage tube is closed, and the other end of the reagent storage tube is open; the open end of the reagent storage tube is provided with an inclined blade, and the closed end is provided with a first groove.

The reagent storage tube is also internally provided with a gravity ball, the second reagent is a freeze-dried ball, and the gravity ball is arranged above the freeze-dried ball.

The gravity ball is a solid with a smooth surface and is made of a high polymer material.

The reaction tube is provided with a first reagent, and the lower half part of the reaction tube is provided with a transparent detection window; the detection window is positioned at the bottom of the reaction tube.

Both ends opening in both ends opening, the both ends opening in additive hole of application of sample hole all have the seal membrane, application of sample hole and reagent hole are circular through-hole.

The sample adding table is provided with a reagent tube positioning groove, the cross section of the tube cover is circular or square, the outer wall of the tube cover is provided with a first bulge, and the first bulge is matched with the reagent tube positioning groove (the matching means that the first bulge can be matched with the reagent tube positioning groove and can position a reagent tube); a rubber sealing plug is fixed in the sampling hole; the lower half part of the reaction tube is flat.

The first half cross section of reaction tube is circular or rectangle, the first half and the tube cap cooperation of reaction tube are connected, the reaction tube opening part is equipped with the seal membrane and makes the one end in application of sample hole and reagent hole sealed, it makes the other end in application of sample hole and reagent hole sealed to be equipped with the seal membrane on the upper surface of tube cap. The round or rectangular tube is convenient to process and take and place, and in addition, the round tube wall can ensure uniform thickness, and the temperature inside the reaction tube is ensured to be stabilized at a target value by heating. The upper part of the structure is wider, the reagent can be ensured to be fully contacted and reacted with the sample, the freeze-dried ball is ensured to be dissolved, and the heat conduction speed and the PCR amplification speed are ensured due to the narrower lower structure.

A detection method of a nucleic acid detection analyzer, characterized in that: the nucleic acid detector comprises a frame, a temperature control device, a fluorescence detection device, a hot cover device and a reagent tube,

the frame comprises a bottom plate, a support frame arranged on the bottom plate, a shell and a sample adding table arranged on the support frame, wherein the sample adding table is provided with an insertion hole;

the hot cover device is arranged on the frame;

the reagent tube is arranged on the insertion hole and is covered by the thermal cover device, the reagent tube is provided with a first reagent and a reagent storage tube separated from the first reagent, and the reagent storage tube is provided with a second reagent;

the position of the reagent tube is matched with the position of the temperature control device; the detection position of the fluorescence detection device is opposite to the reagent tube;

the detection method comprises the following steps:

the method comprises the following steps: adding the sample to the reagent vessel to mix the sample with the first reagent, opening the thermal cover means, placing the reagent vessel in the insertion hole and covering the thermal cover means,

step two: the temperature control device heats and incubates the reaction part of the reagent tube, releases the nucleic acid in the sample into the reagent tube,

step three: adding a second reagent in the reagent storage tube into the reagent tube to be mixed with the sample,

step four: controlling the temperature of the reaction part of the reagent tube by using a temperature control device, so that the mixture of the sample reagent in the reagent tube reaches a first temperature, and the nucleic acid is melted at the first temperature;

step five: controlling the temperature of the reaction part of the reagent tube by using a temperature control device to enable the mixture of the sample reagent in the reagent tube to reach a second temperature, and copying the nucleic acid at the second temperature;

step six: controlling the temperature of the reaction site of the reagent tube with a temperature control device such that the temperature of the mixture of the sample reagents in the reagent tube is cycled between a first temperature and a second temperature until amplification of the nucleic acid is completed;

step seven: the fluorescence detection device optically detects the nucleic acid in the reagent tube.

Further, the method also comprises the following steps: heating the hot cover device; step a is performed simultaneously with or before step six. The heating of the hot cover can prevent the reagent box from being heated unevenly and avoid the condensation phenomenon, and it can be understood that the effect can be achieved when the heating of the hot cover is carried out synchronously with the step six or before the step six, and the effect is best when the heating of the hot cover is completed before the step four or before the step four.

The invention has the beneficial effects that:

according to the invention, a sample is directly added into a special reagent tube, the reagent tube is placed in a specific environment through the hot cover device, a reaction environment is created for the reagent tube through the hot cover device and the temperature control device, and the addition of a second reagent can be realized without opening the hot cover, so that the whole polymerase chain reaction process is realized.

The whole polymerase chain reaction process is totally closed and pollution-free, a special laboratory environment is not needed, only the operation of sample adding is needed, and professional operators are not needed, so the method can be applied to various scenes and has a wide application range.

The reaction process is simple, the reaction time is short, the detection result can be quickly obtained, and the device has small volume and can be carried by a hand bag. These advantages make the invention suitable for bedside detection, further increasing the application range of the invention.

The integrated level of each part of the invention is higher and the parts are mutually independent, namely, a reagent tube, a hot cover device, a temperature control device and an optical detection device are matched on the frame for use, so that a complete detection process can be carried out; therefore, the structure of the present invention can be expanded according to the detection requirement, and transformed into a multi-channel nucleic acid detection analyzer, as shown in fig. 1, two reagent tubes, two thermal cover devices, two temperature control devices and two optical detection devices are installed in a rack, and thus, the structure can be transformed into a nucleic acid detection analyzer with two fluxes (i.e. two samples are detected simultaneously). Similarly, by using the principle of the present invention, the number of samples detected by the nucleic acid detecting analyzer can be increased, such as three channels, four channels, eight channels, etc., which enables the present invention to flexibly change the number of detected channels according to different needs.

Drawings

FIG. 1 is an external structural view of the present invention;

FIG. 2 is a schematic diagram showing the positional relationship of the parts of the present invention;

FIG. 3 is a schematic view of the internal structure of the present invention;

FIG. 4 is a schematic view of the structure of a temperature control device;

FIG. 5 is a schematic diagram of a structure of a movable platform of the temperature control device;

FIG. 6 is a schematic view of the external structure of the thermal cover device;

FIG. 7 is a schematic structural view of a cover shell of the thermal cover device;

FIG. 8 is a schematic view of the internal structure of the thermal cover device;

FIG. 9 is a schematic view of the construction of the heat-conducting plate of the heat-covering device;

FIG. 10 is a schematic view of the structure of a reagent vessel;

fig. 11 is a sectional view of fig. 10.

The reference numbers in the figures denote:

11-a bottom plate, 12-a sample adding table, 13-a support frame and 14-a shell;

2-a temperature control device, 21-a temperature control driving motor, 22-a bearing seat, 23-a screw rod, 24-a screw rod nut, 25-a guide rail, 26-a slide block, 27-a moving platform, 28-a ceramic heating sheet, 29-a heat conduction copper sheet, 210-a trigger part and 211-an inductive switch;

3-a fluorescence detection device;

4-hot cover device, 41-cover shell, 411-upper cover shell, 412-lower cover shell, 413-first installation step, 414-second installation step, 42-heat conduction plate, 421-annular boss, 422-sensor installation groove, 43-guide plate, 431-first elastic element, 432-waist-shaped hole, 441-ejector rod installation frame, 442-linear motor, 443-ejector rod, 444-linear bearing;

5-a reagent tube, 51-a tube cover, 52-a reaction tube, 53-a reaction cavity, 54-a reagent storage tube, 55-a rubber sealing plug, 56-a first bulge, 57-a gravity ball, 58-a freeze-drying ball, 59-an inclined blade and 510-a first groove;

6-display screen.

Detailed Description

The invention provides a method for solving the problems of complex operation, few application scenes, complex structure and high cost of the existing instrument.

As shown in fig. 1, fig. 2 and fig. 3, a nucleic acid detecting analyzer comprises a rack, a temperature control device 2, a fluorescence detecting device 3, a thermal cover device 4 and a reagent tube 5, wherein the rack comprises a bottom plate 11, a support frame 13, a shell 14 and a sample adding table 12, the support frame 13 is arranged on the bottom plate, the sample adding table 12 is arranged on the support frame 13, and an insertion hole is formed in the sample adding table 12; the temperature control device 2 and the fluorescence detection device 3 are arranged between the bottom plate 11 and the sample adding platform 12; the hot cover device 4 is arranged on the frame; the reagent tube 5 is mounted on the insertion hole and covered by the thermal cover device 4; the position of the reagent tube 5 is matched with the position of the temperature control device 2 (the matching mentioned here means that one end of the reagent tube 5 is positioned in the temperature control device after the reagent tube is installed, and the temperature control device can control the temperature in the reagent tube); the detection position of the fluorescence detection device 3 faces the reagent tube 5.

In this embodiment, the thermal cover device 4 is mounted on the sample application station 12.

The one end that the reagent pipe was covered by the heat lid is located application of sample platform top, and the other end is located the below of application of sample platform, and fluorescence detection device is located the below of reagent pipe, and temperature control device is including being used for the moving platform to reagent pipe lower extreme heating, and moving platform is located and adds between sample platform and the fluorescence detection device.

A detection method of a nucleic acid detection analyzer, characterized in that: the nucleic acid detector comprises a frame, a temperature control device 2, a fluorescence detection device 3, a thermal cover device 4 and a reagent tube 5 which are arranged on the frame,

the frame comprises a bottom plate 11, a support frame 13 arranged on the bottom plate, a shell 14 and a sample adding table 12 arranged on the support frame 13, wherein the sample adding table 12 is provided with an insertion hole;

the temperature control device 2 and the fluorescence detection device 3 are arranged between the bottom plate 11 and the sample adding platform 12;

the hot cover device 4 is arranged on the frame;

a reagent tube 5 having a first reagent therein and a reagent holding tube 54 spaced apart from the first reagent therein, the reagent holding tube 54 having a second reagent therein, is mounted on the insertion hole and covered by the thermal cover device 4;

the position of the reagent tube 5 is matched with the position of the temperature control device 2 (the matching mentioned here means that one end of the reagent tube 5 is positioned in the temperature control device after the reagent tube is installed, and the temperature in the reagent tube can be controlled by the temperature control device); the detection position of the fluorescence detection device 3 is opposite to the reagent tube 5;

the detection method comprises the following steps:

the method comprises the following steps: adding the sample to the reagent vessel to mix the sample with the first reagent, opening the thermal cover means 4, placing the reagent vessel in the insertion hole and covering the thermal cover means,

step two: the temperature control device 2 heats and incubates the reaction part of the reagent tube, releases the nucleic acid in the sample into the reagent tube,

step four: controlling the temperature of the reaction site of the reagent vessel with the temperature control device 2 so that the mixture of the sample reagents in the reagent vessel reaches a first temperature at which the nucleic acid is melted;

step five: controlling the temperature of the reaction site of the reagent vessel with the temperature control device 2 so that the mixture of the sample reagents in the reagent vessel reaches a second temperature at which the nucleic acid is replicated;

step six: controlling the temperature of the reaction site of the reagent vessel with the temperature control device 2 so that the temperature of the mixture of the sample reagents in the reagent vessel is cycled between the first temperature and the second temperature until the amplification of the nucleic acid is completed;

step seven: the fluorescence detection device 3 optically detects the nucleic acid in the reagent tube 5.

A display screen 6 may be mounted on the housing, the fluorescence detection device 3 feeds back the optical detection results to the control system, and the control system outputs the optical detection results to the display screen 6.

Further comprising the step of A: heating the hot cover device; step a is performed simultaneously with or before step six. The heating of the hot cover can prevent the reagent box from being heated unevenly and avoid the condensation phenomenon, and it can be understood that the effect can be achieved when the heating of the hot cover is carried out synchronously with the step six or before the step six, and the effect is best when the heating of the hot cover is completed before the step four or before the step four.

The working principle of the invention is as follows:

adding the sample to the reagent vessel to mix the sample with the first reagent, opening the thermal cover means 4, placing the reagent vessel in the insertion hole and covering the thermal cover means,

the hot-cover device is heated up,

the temperature control device 2 heats and incubates the reaction part of the reagent tube, releases the nucleic acid in the sample into the reagent tube,

controlling the temperature of the reaction site of the reagent vessel with the temperature control device 2 so that the mixture of the sample reagents in the reagent vessel reaches a first temperature at which the nucleic acid is melted;

controlling the temperature of the reaction site of the reagent vessel with the temperature control device 2 so that the mixture of the sample reagents in the reagent vessel reaches a second temperature at which the nucleic acid is replicated;

controlling the temperature of the reaction site of the reagent vessel with the temperature control device 2 so that the temperature of the mixture of the sample reagents in the reagent vessel is cycled between the first temperature and the second temperature until the amplification of the nucleic acid is completed;

the fluorescence detection device 3 optically detects the nucleic acid in the reagent tube 5;

the fluorescence detection device 3 feeds back the optical detection result to the control system, and the control system outputs the optical detection result to the display screen.

According to the invention, a sample is directly added into a special reagent tube, the reagent tube is placed in a specific environment through the hot cover device, a reaction environment is created for the reagent tube through the hot cover device and the temperature control device 2, and the addition of a second reagent can be realized without opening the hot cover, so that the whole polymerase chain reaction process is realized.

Temperature control device

This section describes in detail the specific structure of the temperature control section and how the temperature control device controls the temperature in the reagent tube.

As shown in fig. 4 and 5, the temperature control device 2 comprises a driving component, a guiding component and a first heating component,

the driving assembly comprises a temperature control driving motor 21, a bearing seat 22, a lead screw 23 and a lead screw nut 24, wherein the temperature control driving motor 21 is installed on the rack;

the guide assembly comprises two guide rails 25 mounted on the frame;

the first heating assembly comprises a moving platform 27 arranged on the lead screw nut 24, and a sliding block 26 connected with the guide rail 25 in a sliding fit manner is arranged on the moving platform 27;

the moving platform 27 is provided with four heating grooves for heating, the four heating grooves are arranged in parallel with the guide rail 25, each heating groove is internally provided with a ceramic heating sheet 28 and two heat conduction copper sheets 29 tightly abutted to the ceramic heating sheet 28, a gap for the reagent tube 5 to pass through is formed between the two heat conduction copper sheets 29, and the heat conduction copper sheets 29 are attached to the outer wall of the reagent tube 5.

The temperatures in the four heating tanks are different. The temperatures in the four heating tanks include a first target temperature and a second target temperature for polymerase chain reaction, and a first transition temperature and a second transition temperature for transition, and the values of the first transition temperature, the first target temperature, the second target temperature, and the second transition temperature are sequentially reduced.

The moving platform 27 is a heat insulating material. The moving platform 27 is formed by installing two heat insulation plates which are connected with each other. The temperature controlled drive motor 21 is a stepping motor.

The movable platform 27 is provided with a trigger 210, the rack is provided with an inductive switch, and the position of the trigger 210 is matched with that of the inductive switch; or the frame is provided with a trigger 210, the moving platform 27 is provided with an inductive switch, and the trigger 210 is matched with the position of the inductive switch (where matching refers to relative movement between the trigger 210 and the inductive switch to enable the inductive switch to be controlled to be triggered).

A method of polymerase chain reaction comprising the steps of:

the method comprises the following steps: placing the reagent tube filled with the nucleic acid in a first transition temperature, and heating;

step two: placing the reagent tube containing the nucleic acid at a first target temperature to perform a melting reaction;

step three: placing the reagent tube after the melting reaction at a second transition temperature, and cooling;

step four: placing the cooled reagent tube at a second target temperature to copy nucleic acid;

step five: circulating the first step to the fourth step;

step six: repeating the step one once or repeating the step one to the step four once;

in the above steps, the values of the first transition temperature, the first target temperature, the second target temperature, and the second transition temperature are sequentially decreased.

In another embodiment, a method of polymerase chain reaction, comprising the steps of:

the method comprises the following steps: placing the reagent tube containing the nucleic acid at a first target temperature to perform a melting reaction;

step two: placing the reagent tube after the melting reaction at a second transition temperature, and cooling;

step three: placing the cooled reagent tube at a second target temperature to copy nucleic acid;

step four: placing the reagent tube with the copied nucleic acid in a first transition temperature, and heating;

step five: circulating the first step to the fourth step;

step six: repeating the step one to the step three once or repeating the step one to the step four once;

The first transition temperature is at least five degrees centigrade higher than the first target temperature, and the second transition temperature is at least ten degrees centigrade lower than the second target temperature.

In the first step, the difference between the temperature in the reagent tube after temperature reduction and the second target temperature is at most ten degrees centigrade.

In the third step, the difference between the temperature in the reagent tube after temperature rise and the first target temperature is at most ten degrees centigrade.

In step one, the temperature in the reagent tube after cooling is at most five degrees celsius higher than the second target temperature.

In step three, the temperature in the reagent tube after the temperature rise is lower than the first target temperature by at most five degrees centigrade.

The working principle of the temperature control device is as follows:

the ceramic heating sheets 28 in different heating grooves are set to different temperatures, and the temperatures of the ceramic heating sheets are conducted to the heat-conducting copper sheets 29, so that the temperatures of the heat-conducting copper sheets 29 in different heating grooves are different.

After the reagent tube is arranged on the sample adding table, a part of the reagent tube 5 is positioned in a gap between the two heat-conducting copper sheets 29, and the copper sheets and the reagent tube 5 can exchange heat to realize temperature rise or temperature reduction in the reagent tube.

The temperature control driving motor 21 of the invention rotates to drive the screw 23 to rotate, and the screw nut 24 drives the moving platform 27 to move on the guide rail 25, so that the reagent tube 5 is positioned in different heating grooves, the temperatures in different heating grooves are different, the reagent tube reaches different temperatures, and the polymerase chain reaction is realized.

The reagent tube 5 is fixed, and the reagent tube 5 can be switched in different temperature areas through the reciprocating motion of the moving platform, so that the reagent tube 5 and the reagents in the reagent tube are kept in a stable state, and the consistency of each reaction is facilitated. Meanwhile, compared with the heating or cooling at the same position, the reagent tube 5 directly enters another temperature zone, so that the stable change speed is higher.

The number of the heating grooves is at least three, which is beneficial to further improving the change speed of the temperature, and the specific principle is as follows:

the temperatures used for the reaction are two, namely: the first target temperature is used for melting and the second target temperature is used for replication. At least two heating tanks are required, which are maintained at the first target temperature and the second target temperature, respectively. Since the rate of heat transfer is related to the temperature difference, when the temperature difference is small, particularly when the temperature is about to reach the target temperature, the rate of heat transfer is significantly reduced, which results in a slow rate at the time of temperature switching, extending the time required for the reaction. This problem has a great influence on the whole reaction process because one cycle process requires at least two temperature switching and the whole reaction process requires several tens of cycles.

And when the temperature of the reagent tube needs to be switched from the first reaction temperature to the second reaction temperature, the reagent tube is firstly placed in the environment of the second transition temperature, so that the temperature of the reagent tube is reduced to the second reaction temperature, and then the reagent tube is placed in the environment of the second reaction temperature, so that the reagent tube is stabilized at the second reaction temperature. The process can be realized by setting the temperatures of the three heating tanks to the first reaction temperature, the second reaction temperature and the second transition temperature, respectively. The temperature difference between the first target temperature and the second transition temperature is large, so that the heat conduction speed is high, the temperature switching speed is increased, and the reaction time is shortened.

Similarly, when the temperature of the reagent tube needs to be switched from the second reaction temperature to the first reaction temperature, the reagent tube is placed in the environment of the first transition temperature, the temperature of the reagent tube is raised to the first reaction temperature, and the reagent tube is stabilized in the first reaction temperature in the environment of the first reaction temperature.

In this embodiment, four heating tanks are provided, and the temperatures in the four heating tanks are respectively: a first reaction temperature, a second reaction temperature, a first transition temperature, and a second transition temperature. The temperature changing method can realize rapid temperature changing and carry out multiple cycles.

The two heat-conducting copper sheets 29 are used for heating or cooling two sides of the reagent tube, and the temperature changing speed can be increased in such a way.

The moving platform 27 is made of heat insulating material, so that the reagent tube can only exchange heat through the heat conducting copper sheet, the temperature change speed can be increased, and the temperature in the heating tank can be kept stable.

The heat conduction copper sheet is attached to the reagent tube, so that heat of the heat conduction copper sheet is directly transmitted to the reagent tube, and the problems of low speed and large heat loss caused by air conduction are solved.

The temperature control driving motor is a stepping motor, and can control the moving distance of the moving platform through the temperature control driving motor; the trigger 210 can accurately feed back the position of the mobile platform, thereby positioning the mobile platform to the initial position.

Different heating tanks set independently, can not conduct heat between the heating tanks, also set independently between reagent pipe and the heating tank, and the state is stable, can not produce the pollution, and it is simple to maintain, need not to maintain even.

The temperature control device has compact structure and small volume.

The bearing seat 22, the temperature control driving motor 21, the lead screw 23 and the guide rail 25 are all arranged on the lower surface of the sample adding table 12. The installation mode can save space, and the reagent tube, the guide rail 25 and the lead screw 23 all use the sample adding platform 12 as a positioning reference, so that the relative position precision among the three can be conveniently improved.

The motion mode of the mobile platform in this embodiment is as follows: the motor drives the lead screw 22 to rotate, and the moving platform 27 is driven to move linearly through the lead screw nut. Other ways, such as a screw motor or a linear motor, can be used to directly drive the moving platform to slide on the guide rail 25.

The supporting frame 13 is an aluminum product, and the center of the supporting frame 13 is hollowed.

The aluminum product has good strength and light weight, and the center of the aluminum product is hollowed out, so that the weight of the aluminum product can be further reduced.

Hot lid device

This section details the specific structure of the thermal cap, the role of the thermal cap, and the role of the thermal cap in instrument automation.

As shown in fig. 6, 7, 8 and 9, the hot lid device 4 includes a lid housing 41, a second heating assembly mounted on the lid housing 41, a guide structure, and a push rod structure.

The cover case 41 includes an upper cover case 411 and a lower cover case 412 cooperatively connected with the upper cover case 411, both the upper cover case 411 and the lower cover case 412 are hollow structures, the upper end of the upper cover case 411 is sealed, and the inner wall of the lower cover case 412 is provided with a first mounting step 413 and a second mounting step 414.

The second heating assembly comprises a heat conducting plate 42 and a heating element contacted with the heat conducting plate 42, the heat conducting plate is provided with a first mandril through hole, the lower surface of the heat conducting plate 42 is provided with an annular boss 421 protruding downwards, the shape of the annular boss 421 is matched with the end part of the reagent tube (the shape of the annular boss 421 is matched with that of the annular boss 421, the end part of the reagent tube can be surrounded by the annular boss 421, so that the heating space covers the end part of the reagent tube), and the inner wall of the annular boss 421 and the lower surface of the heat conducting plate 42 surround a heating space for heating the end part of the reagent tube;

the second heating element comprises a heat-conducting plate 42 and a heating element in contact with the heat-conducting plate 42, a first ejector rod via hole is formed in the heat-conducting plate, a fourth groove is formed in the lower surface of the heat-conducting plate 42, a heating space for heating the end portion of the reagent tube is formed, and the shape of the fourth groove is matched with the end portion of the reagent tube. (adapted as referred to herein means that the fourth groove is capable of enclosing the end of the reagent vessel such that the heating space covers the end of the reagent vessel.)

The heat conducting plate 42 is provided with three guide holes;

the heat conducting plate 42 is provided with a sensor mounting groove 422, and a temperature sensor is mounted in the sensor mounting groove;

the heating element is a PI heating film, which is adhered to the upper surface of the heat conductive plate 42;

the guide structure comprises a guide plate 43, three guide shafts arranged on the lower surface of the guide plate 43 and a first elastic part 431 sleeved on the guide shafts, the guide plate 43 is provided with a second ejector rod through hole and a waist-shaped hole 432,

the kidney-shaped hole 432 is used for threading, and the heating film and the temperature sensor need to be connected by a circuit.

The guiding axle adopts stainless steel, and in order to guarantee that the motion is smooth and easy, the roughness on its surface is extremely low, and the end of guiding axle is the recess, is convenient for fix spacing with the jump ring.

The ejector rod structure comprises an ejector rod mounting frame 441 arranged on the guide plate 43, a linear motor 442 arranged on the ejector rod mounting frame 441, and an ejector rod 443 which is arranged on the ejector rod mounting frame 441 in a sliding manner and is in fit connection with a sliding block of the linear motor 442;

a linear bearing 444 is installed on the mounting frame of the ejector rod 441, and an ejector rod 443 is installed on the linear bearing 444;

and a third groove is formed in the middle of the ejector rod and is connected with a sliding block of the linear motor in a matching manner.

The end part of the ejector rod is provided with an ejector rod boss. (the ejector rod boss is used for puncturing a sealing film at a position corresponding to the reagent storage tube and is in fit connection with the first groove 510 at the top of the reagent storage tube so as to ensure that the reagent storage tube is vertically stressed downwards and is pushed to move downwards.)

The guide plate 43 of the guide structure is installed on the first step 413, the heat conductive plate 42 of the second heating assembly is located on the second step 414, the guide hole on the heat conductive plate 42 is fittingly connected with the guide shaft, and the first elastic member 431 is located between the heat conductive plate 42 and the guide plate 43, and the end portion of the ejector 443 passes through the first ejector pin through hole and the second ejector pin through hole and is located in the heating space.

The principle of the hot cover device is as follows:

the reagent tube is mounted on the sample application table, and the thermal cover is covered on the reagent tube, so that the reagent tube is covered in the machine and one end of the reagent tube is positioned in the heating space. The specific process is as follows: when the lower surface of the heat conductive plate 42 comes into contact with the end of the reagent tube 5, the heat conductive plate 42 receives a reaction force, so that the heat conductive plate 42 moves toward the guide plate 43 along the guide shaft and the first elastic member 431 is compressed until the heat cover reaches a designated position. Since the first elastic member is in a compressed state, the elastic force pushes the heat conducting plate 42 to press the reagent tube, ensuring that the reagent tube 5 is positioned at a designated position for heating, reacting and detecting the reagent tube 5. The mode that can open and close that adopts commonly used between hot lid and the application of sample platform for example adopt modes such as buckle or magnetism to guarantee that hot lid covers on the application of sample platform.

After the cover reaches the designated position, the heating elements heat the plate 42 to maintain the heating space at the desired temperature. On one hand, the sample can react at the temperature to expose the neutralized nucleic acid in the sample, and on the other hand, the whole reagent tube is in a higher temperature environment, so that the condensation phenomenon can be effectively avoided. The temperature of the heating space is fed back through the first temperature sensor, the temperature of the general heating space is kept at 90-110 ℃, and the effect of preventing condensation is good.

When a second reagent needs to be added, the linear motor 442 moves to drive the ejector rod 443 to slide in the linear bearing 444, the boss of the ejector rod can puncture the sealing film at the position corresponding to the position of the reagent storage tube and push the reagent storage tube to move downwards, so that the second reagent moves downwards into the reaction solution; the ejector pin 443 then returns to the home position.

And after the detection is finished, the hot cover is opened, and the reagent tube is taken out, so that the whole detection process is finished.

The beneficial effect of hot lid device lies in:

because the heat conducting plate 42 is used for covering the end part of the reagent tube and the heat conducting plate 42 can be heated, the reagent tube 5 can be ensured to carry out amplification reaction and optical detection, and meanwhile, the phenomenon of condensation caused by uneven heating can not occur.

Because heat-conducting plate 42 can slide to pressing reagent pipe under the elasticity effect of first elastic component, can compressing tightly the reagent pipe, guarantee that the other end of reagent pipe is located temperature control device 2, and tightly support with fluorescence detection device 3, thereby provide the guarantee for follow-up reaction and detection. Meanwhile, the heat conducting plate 42 can slide to adapt to reagent tubes with different heights, so that the reagent tubes with different heights can be tightly pressed, and the requirement on the processing precision of the reagent tubes and the heating plate is greatly reduced.

Because the push rod is arranged in the hot cover device, and the push rod 443 can reciprocate to pierce the sealing film, the movement of the reagent storage tube and the addition of a second reagent are realized, the process is completely finished by an instrument without manual operation, and the hot cover does not need to be opened, so that the problem that a sample is polluted in the reaction process is thoroughly solved, the whole reaction process is carried out in a stable environment, and the stability of the reaction and the accuracy of a detection result are facilitated.

The nucleic acid detection analyzer is provided with a cover for adding the reagent tube or the sample into the instrument, and the compressing device, the heating device and the automatic adding device of the second reagent of the reagent tube are integrated on the cover, so that the space is greatly saved, the structure of the instrument is simplified, the operation steps are simplified, and the use experience of a user is improved.

Since the structure inside the thermal cover device is large, dividing the cover case 41 into two parts facilitates the installation of the internal structure of the thermal cover device.

The processing difficulty of the first and second mounting

steps

413 and 414 is small, and the difficulty of the remaining structure on the first and second mounting

steps

413 and 414 is small.

The heating space is created by means of the annular projection 421 or the fourth recess, which are both simple to manufacture and do not require additional parts to be mounted, and which facilitate the conduction of heat from the heating element to the heating space.

The PI heating film is adopted for heating, so that a structure special for mounting a heating element can be avoided being processed on the heat conducting plate.

The guide plate 43 is used to mount the ram structure and guide the second heating assembly.

Because the heat-conducting plate 42 is guided by the guiding shaft, the heat-conducting plate 42 is limited by the second step in the axial direction of the guiding shaft, and the processing precision requirement and the installation difficulty are greatly reduced. The specific reasons are as follows: if the limit structure of the heat conducting plate 42 in the axial direction of the guide shaft is arranged on the guide shaft, the matching precision of the guide shaft and the guide hole and the position precision and the installation precision of the limit structure need to be ensured at the same time, the processing precision requirement is very high, and the installation difficulty is high because the inner space of the heat cover device is small; similarly, if the structure for guiding is arranged on the cover shell, the processing precision requirement of the cover shell is very high, and the processing difficulty is higher considering that the structure of the cover shell is relatively thin; in this scheme, only need consider the guiding axle with the cooperation precision of guiding hole can, only need on the lid shell guarantee limit structure's position precision can, the processing degree of difficulty is very little.

The linear motor 442 is used for driving the ejector rod to move, and a sliding block of the linear motor is connected with the third groove of the ejector rod 443 in a matched mode. Other common transmission structures can be adopted to replace the transmission mechanism, for example, a rotating motor is adopted to be matched with a gear rack or a worm wheel and worm or a lead screw sliding rail to carry out transmission; or an air pump or a hydraulic pump is used for driving the mandril to move.

Reagent tube

This section details the specific structure of the reagent vessel, how mixing of the first and second reagents is automatically achieved in the reagent vessel, and other functions of the reagent vessel.

As shown in fig. 10 and 11, a reagent tube includes a tube cap 51 and a reaction tube 52, wherein the tube cap 51 is provided with a sample adding hole and a reagent adding hole; a reagent storage tube 54 is arranged in the reagent adding hole, a second reagent is arranged in the reagent storage tube, and a sealing film for sealing the sample adding hole and the reagent adding hole is arranged on the tube cover 51; a reaction cavity 53 is arranged in the reaction tube 52; the tube cap 51 and the reaction tube 52 are connected in a matching way to make the reaction chamber in a sealed state.

The cross section of the cap 51 is circular or square. Round or square is convenient to grab. The sample adding table is provided with a reagent tube positioning groove, the outer wall of the tube cover is provided with a first bulge 56, and the first bulge is matched with the reagent tube positioning groove.

A rubber sealing plug 55 is fixed in the sample application hole. The inner wall of the sample adding hole is provided with a second bulge, and the rubber sealing plug 55 is provided with a second groove matched with the second bulge.

The reagent storage tube 54 is movably installed in the reagent adding hole of the tube cap 51.

The reagent storage tube is tubular, one end of the reagent storage tube is closed, and the other end of the reagent storage tube is open.

The open end of the reagent holding tube 54 has a beveled edge 59 and the closed end is provided with a first recess 510.

The reagent holding tube 54 also has a gravity ball 57 therein, the second reagent is a lyophilized ball 58, and the gravity ball 57 is disposed above the lyophilized ball 58.

The reagent storage tube 54 further has a second elastic member therein, the second reagent is a freeze-dried ball 58, the lower end of the second elastic member abuts against the freeze-dried ball, and the upper end of the second elastic member abuts against the reagent storage tube.

The second elastic piece is a plastic spring.

The plastic spring is pre-pressed in the reagent storage tube, so that the vibration of the freeze-drying ball in the transportation process can be reduced, and when the sealing film on the reaction tube is punctured, the force for ejecting the freeze-drying ball can be given, so that the freeze-drying ball can fall into the solution more smoothly.

The two ends of the sample adding hole and the two ends of the reagent adding hole are provided with sealing films, and the sealing films can be membranes easy to puncture.

The sampling hole and the reagent hole are both circular through holes.

The reaction tube has a first reagent therein.

The lower half of the reaction tube 52 is flat and has a transparent detection window.

The detection window is located at the bottom of the reaction tube 52.

The first half cross section of reaction tube 52 is circular or rectangle, and the first half of reaction tube 52 is connected with the cooperation of tube cap 51, and the opening part of reaction tube 52 is equipped with the seal membrane and makes the one end in addition hole and reagent hole sealed, is equipped with the seal membrane on the upper surface of tube cap 51 and makes the other end in addition hole and reagent hole sealed. The round or rectangular tube is convenient to process and take and place, and in addition, the round tube wall can ensure uniform thickness, and the temperature inside the reaction tube is ensured to be stabilized at a target value by heating. The upper part of the structure is wider, the reagent can be ensured to be fully contacted and reacted with the sample, the freeze-dried ball is ensured to be dissolved, and the heat conduction speed and the PCR amplification speed are ensured due to the narrower lower structure.

The working principle of the reagent tube is as follows: during sample adding, the syringe pierces the seal on the tube cover 51, the rubber sealing plug and the sealing film on the reaction tube 52, and enters the first reagent for sample adding, and when the syringe is taken out, the rubber sealing plug is automatically sealed through elasticity.

Because the reaction tube is provided with the first reagent, the sample can firstly react with the first reagent, and because the tube cover is provided with the second reagent, the second reagent can be timely added into the reaction tube to carry out the next reaction on the sample. And after the reaction is finished, the detection device detects through a detection window at the bottom of the reaction tube.

The working principle of adding the second reagent into the reaction tube is as follows: the reagent storage tube is pushed, and the sealing film on the reaction tube can be punctured by the inclined blade of the reagent storage tube, so that the second reagent enters the reagent reaction tube and is mixed with the first reagent and the sample.

The instrument can be provided with an ejector rod, the reagent tube is arranged in the reaction cavity, and the ejector rod moves to push the reagent storage tube.

The second reagent in this embodiment is stored in the form of a lyophilized pellet, which has the benefits of:

when the sealing membrane is punctured to the inclined edge of reagent storage tube, freeze-drying ball and gravity ball all can fall into in the reaction tube, and the gravity of gravity ball can guarantee that freeze-drying ball can fall and invade in the first reagent completely.

After the freeze-drying ball is completely dissolved, the instrument heats the thin wall of the lower part of the reaction tube, PCR reaction is started, and finally detection is carried out through a detection window at the bottom of the reaction tube.

The reagent tube has the beneficial effects that:

because the reagent tube of the invention is provided with the first reagent and the second reagent at the same time and can respectively react with the sample, the extraction of nucleic acid and the PCR amplification reaction can be carried out in the reagent tube, the transfer of nucleic acid is avoided, and the cost and the complexity of the instrument are greatly reduced;

the second reagent can be added under the condition that the reagent tube is not opened, so that the operation steps are reduced, and the risk of sample pollution is reduced;

the rubber sealing plug is arranged in the sample adding hole of the reagent tube, so that the sample adding of the public injector is facilitated, and the rubber sealing plug is automatically sealed through the elasticity of the rubber plug after the sample adding, so that the sealing property of the reaction tube is improved;

the sealing films of the sample adding hole and the reagent hole can ensure that the interior of the reagent tube is in a sealing state, so that the reagent tube is prevented from being polluted, and the detection accuracy is improved;

the second protrusion is matched with the second groove of the rubber sealing plug 55, so that the rubber sealing plug 55 is ensured not to slide in the sample adding hole, the puncture of the sample adding needle is ensured to be smoothly carried out, and the condition that the rubber sealing plug 55 is moved to damage the sealing film is also avoided.

First arch on the tube cap outer wall is used for realizing the location with the instrument cooperation, the installation of being convenient for to guarantee that reagent pipe can install and react and detect in appointed position.

The reagent storage tube is used for storing and releasing a second reagent, when the second reagent is released, the blade tip of the inclined blade pierces one point of the sealing membrane, the reagent storage tube continues to move, and the edge of the inclined blade tears the sealing membrane, so that the reagent storage tube can penetrate through the sealing membrane, and the second reagent enters the reaction tube. The sealing membrane is only punctured at one point contacting with the cutting edge of the inclined blade, and the membrane at the far end of the cutting edge is not torn and is connected with the original membrane body, so that the sealing membrane is prevented from falling into the reagent.

The freeze-drying ball is matched with the reagent storage tube, so that the condition that liquid reagents are easy to flow and seep is not considered, the freeze preservation is invalid, the second reagents can be conveniently loaded, sealed, stored and transported, and the convenience of the reagent tube and the instrument is improved.

The gravity ball is arranged above the freeze-drying ball, so that gaps in the reagent storage tube can be filled, and the freeze-drying ball is prevented from being cracked due to vibration in the tube in the transportation process. The gravity ball can press the freeze-drying ball through gravity down, makes the freeze-drying ball fall smoothly from the reagent storage tube, and can push down the freeze-drying ball after the whereabouts, increases the area of contact of freeze-drying ball and first reagent for reagent fully dissolves.

The lower part of the reaction tube is designed to be flat, so that the heating module can be completely attached, and the lower part of the reaction tube is of a thin-wall structure, so that the heating efficiency is further increased, and finally, the detection time is greatly shortened.

The PCR detection reagent tube has simple integral structure and small volume.

Claims

1. A nucleic acid detection analyzer comprises a frame, and is characterized in that: the device also comprises a temperature control device, a fluorescence detection device, a hot cover device and a reagent tube which are arranged on the frame; the frame comprises a bottom plate and a sample adding table arranged on the bottom plate, and the sample adding table is provided with an insertion hole; the temperature control device and the fluorescence detection device are arranged between the bottom plate and the sample adding platform; the hot cover device is arranged on the frame; the reagent tube is arranged on the insertion hole and is covered by the thermal cover device; the position of the reagent tube is matched with the position of the temperature control device; the detection position of the fluorescence detection device is opposite to the reagent tube.

2. The nucleic acid detecting analyzer according to claim 1, wherein: the one end that reagent pipe was covered by the heat lid is located application of sample platform top, the other end is located the below of application of sample platform, fluorescence detection device is located the below of reagent pipe, temperature control device is including being used for the moving platform to reagent pipe lower extreme heating, moving platform is located between application of sample platform and the fluorescence detection device.

3. The nucleic acid detecting analyzer according to claim 1 or 2, wherein: the number of the temperature control devices, the number of the fluorescence detection devices, the number of the thermal cover devices and the number of the reagent tubes are at least two, and the temperature control devices, the fluorescence detection devices, the number of the thermal cover devices and the number of the reagent tubes are matched in a one-to-one correspondence mode.

4. The nucleic acid detecting analyzer according to claim 1, 2 or 3, wherein: the frame is still including installing support frame, the shell on the bottom plate, add the appearance platform and install on the support frame, hot lid device is installed on adding the appearance platform.

5. The nucleic acid detecting analyzer according to claim 1, 2, 3 or 4, wherein: the temperature control device comprises a driving component, a guide component and a first heating component; the first heating assembly comprises a moving platform, more than three heating grooves for heating are formed in the moving platform, the end portion of the reagent tube is located in one heating groove, and the moving platform is driven by the driving assembly to move along the guide assembly, so that the end portion of the reagent tube is located in different heating grooves.

6. The nucleic acid detecting analyzer according to claim 5, wherein: the driving assembly comprises a temperature control driving motor, a bearing seat, a lead screw and a lead screw nut, wherein the temperature control driving motor is arranged on the rack; the guide assembly comprises two guide rails arranged on the frame; the moving platform is installed on the screw nut, and the moving platform is further provided with a sliding block connected with the guide rail in a sliding fit mode.

7. The nucleic acid detecting analyzer according to claim 6, wherein: the mobile platform is a thermally insulating material; the mobile platform is formed by installing two mutually connected heat insulation plates; the temperature control driving motor is a stepping motor;

the mobile platform is provided with a trigger piece, the rack is provided with an induction switch, and the position of the trigger piece is matched with that of the induction switch; or the frame is provided with a trigger, the mobile platform is provided with an inductive switch, and the trigger is matched with the inductive switch in position.

8. The nucleic acid detecting analyzer according to claim 5, wherein: the quantity of heating groove is four, four heating groove and guide rail parallel arrangement, every all install ceramic heating piece and the heat conduction copper sheet that tightly supports with ceramic heating piece in the heating groove, every heat conduction copper sheet quantity in the heating groove is two, two have the clearance that is used for the reagent pipe to pass through between the heat conduction copper sheet, the heat conduction copper sheet pastes with the outer wall of reagent pipe mutually.

9. The nucleic acid detecting analyzer according to claim 8, wherein: the four temperatures in the heating tank comprise a first target temperature and a second target temperature for polymerase chain reaction, a first transition temperature and a second transition temperature for transition, and numerical values of the first transition temperature, the first target temperature, the second target temperature and the second transition temperature are sequentially reduced.

10. The nucleic acid detecting analyzer as set forth in claim 1, 2, 3, 4, 6, 7, 8 or 9, wherein: the hot cover device comprises a cover shell and a second heating assembly arranged on the cover shell; the second heating element includes the heat-conducting plate and with the heating element of heat-conducting plate contact, the lower surface of heat-conducting plate has the annular boss of downward salient, the shape of annular boss and the tip looks adaptation of reagent pipe, the inner wall of annular boss encloses the heating space who becomes to be used for the tip heating of reagent pipe with the lower surface of heat-conducting plate.

11. The nucleic acid detecting analyzer according to claim 10, wherein: the heat cover device also comprises a guide structure arranged on the cover shell, wherein the guide structure comprises a guide plate, a guide shaft arranged on the lower surface of the guide plate and a first elastic part, and the first elastic part is positioned between the heat conduction plate and the guide plate; the heat-conducting plate is provided with a guide hole, and the guide hole is connected with the guide shaft in a matching manner.

12. The nucleic acid detecting analyzer according to claim 11, wherein: the heat-conducting plate is provided with a first ejector rod through hole, and the guide plate is provided with a second ejector rod through hole and a waist-shaped hole.

13. The nucleic acid detecting analyzer according to claim 11, wherein: the cover shell comprises an upper cover shell and a lower cover shell which is matched and connected with the upper cover shell, the upper cover shell and the lower cover shell are both of a hollow structure, the upper end of the upper cover shell is sealed, and a first mounting step and a second mounting step are arranged on the inner wall of the lower cover shell; the guide plate is installed on the first step, and the heat-conducting plate is located on the second step.

14. The nucleic acid detecting analyzer according to claim 10, wherein: the hot lid device still includes the ejector pin structure, the ejector pin structure is including installing the ejector pin mounting bracket on the lid shell, installing ejector pin driving piece on the ejector pin mounting bracket, the ejector pin that slides and set up on the ejector pin mounting bracket and be connected with the cooperation of ejector pin driving piece, the driving piece can drive the tip of ejector pin gets into in the heating space.

15. The nucleic acid detecting analyzer according to claim 14, wherein: preferably, the ejector rod driving part is a linear motor, a linear bearing is installed on the ejector rod installation frame, and the ejector rod is installed on the linear bearing; a third groove is formed in the middle of the ejector rod and is connected with a sliding block of the linear motor in a matched mode; the end part of the ejector rod is provided with an ejector rod boss.

16. The nucleic acid detecting analyzer as set forth in claim 1, 2, 3, 4, 6, 7, 8, 9, 11, 12, 13, 14, or 15, wherein: the reagent tube comprises a tube cover and a reaction tube, wherein the tube cover is provided with a sample adding hole and a reagent adding hole which are used for communicating the reaction cavity; a reagent storage tube is arranged in the reagent adding hole, a second reagent is arranged in the reagent storage tube, and a sealing film for sealing the sample adding hole and the reagent adding hole is arranged on the tube cover; the reaction tube is internally provided with a reaction cavity; the tube cover is matched and connected with the reaction tube to enable the reaction cavity to be in a sealed state.

17. The nucleic acid detecting analyzer according to claim 16, wherein: the reagent storage tube is movably arranged in the reagent adding hole of the tube cover.

18. The nucleic acid detecting analyzer according to claim 16, wherein: the reagent storage tube is tubular, one end of the reagent storage tube is closed, and the other end of the reagent storage tube is open; the open end of the reagent storage tube is provided with an inclined blade, and the closed end is provided with a first groove.

19. The nucleic acid detecting analyzer according to claim 16, wherein: the reagent storage tube is also internally provided with a gravity ball, the second reagent is a freeze-dried ball, and the gravity ball is arranged above the freeze-dried ball.

20. The nucleic acid detecting analyzer according to claim 19, wherein: the gravity ball is a solid with a smooth surface and is made of a high polymer material.

21. The nucleic acid detecting analyzer according to claim 16, wherein: the reaction tube is provided with a first reagent, and the lower half part of the reaction tube is provided with a transparent detection window; the detection window is positioned at the bottom of the reaction tube.

22. The nucleic acid detecting analyzer according to claim 16, wherein: both ends opening in both ends opening, the both ends opening in additive hole of application of sample hole all have the seal membrane, application of sample hole and reagent hole are circular through-hole.

23. The nucleic acid detecting analyzer according to claim 16, wherein: the sample adding table is provided with a reagent tube positioning groove, the cross section of the tube cover is circular or square, the outer wall of the tube cover is provided with a first bulge, and the first bulge is matched with the reagent tube positioning groove; a rubber sealing plug is fixed in the sampling hole; the lower half part of the reaction tube is flat.