CN115651819A - Self-adaptive top pressure assembly for kit and detection device - Google Patents
Self-adaptive top pressure assembly for kit and detection device Download PDFInfo
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Abstract
The invention discloses a self-adaptive top pressure assembly for a kit, which comprises a driving mechanism and a pressing plate mechanism, wherein the pressing plate mechanism comprises: an actuating platen that performs reciprocating linear motion in a set direction; the passive pressing plate is used for jacking and pressing the reagent kit; the elastic adjusting component comprises a connecting piece and an elastic piece and is arranged between the actuating pressure plate and the driven pressure plate; the actuating pressure plate and the connecting piece can be arranged in a relatively movable manner; the elastic part always applies force to the actuating pressure plate 5 The connecting piece is provided with a first limiting piece used for limiting the actuating pressure plate; the minimum top pressure acting force Pmin and the maximum top pressure acting force Pmax required by the kit; the acting force of the elastic piece on the passive pressure plate has a minimum value Fmin and a maximum value Fmax, and Pmin < Fmin < Fmax < Pmax. The detection device comprising the jacking assembly is also disclosed. When the invention is applied to the top pressure operation of the reagent box, the invention can adapt to the size change of the reagent box caused by the influence of tolerance and temperature,the kit is pressed in place without excessive pressing.
Description
Technical Field
The invention belongs to the field of biological detection devices, and particularly relates to a self-adaptive top pressure assembly for a kit and a detection device.
Background
PCR is the abbreviation of polymerase chain reaction, in the field of biological detection, it is also called acellular molecular clone or specific DNA sequence in vitro primer directional enzymatic amplification technology, and it is a method for in vitro enzymatic synthesis of specific DNA fragment. The method comprises a period consisting of reactions such as high-temperature denaturation, low-temperature annealing, isothermal extension and the like, wherein the period is performed in a circulating manner, so that the target DNA can be amplified rapidly. It has the features of high specificity, high sensitivity, simple operation, saving in time, etc. and may be used in the basic research of gene separation, cloning, nucleic acid sequence analysis, etc. and in the diagnosis of diseases and in any place with DNA and RNA. At present, the amplification technology is widely applied to nucleic acid detection. In actual operation, the problem of cross contamination of amplification products is easy to occur, that is, a negative sample is contaminated by a positive substance to cause false positive reaction, so that an error detection report can be obtained, and great troubles and troubles are caused to detected personnel. In order to solve the above problems in the related art, a dedicated reagent cartridge is designed, which includes two parts that are slidable relative to each other, and a reagent tube containing an amplification product is sealed with an elastic member and placed in one of the parts of the reagent cartridge. When the detection is carried out, the sealing structure on the reagent tube is punctured by the puncture structure arranged at the other part of the kit, so that the cross contamination of the nucleic acid amplification products can be prevented.
In order to make the two parts of the reagent box slide relatively to realize piercing, a top pressing component is needed to top press the reagent box, and the top pressing component in the related art generally uses a linear driving mechanism to drive a pressing plate to do reciprocating linear motion so as to top press the reagent box. In order to ensure the puncture effect, the kit has very high requirements on the top pressure degree: the jacking degree is insufficient, so that the puncture structure cannot enter completely easily, and cannot be matched with the elastic part to realize sealing and cause leakage; excessive jacking easily causes the elastic piece to be excessively extruded and deformed, and causes the liquid in the pipe to be unable to be pumped. In the related art, the reciprocating linear motion stroke of the pressure plate is preset, and the reagent box has inevitable dimensional tolerance during manufacturing, and is influenced by the heating environment during detection and also has dimensional change due to temperature change. Therefore, when the kit is pressed, part of the kit can be pressed incompletely or excessively, and the detection result is greatly influenced.
Disclosure of Invention
The present invention is directed to solve at least one of the above technical problems in the related art to a certain extent, and therefore, the present invention adopts the following technical solutions: the utility model provides a kit is with self-adaptation roof pressure subassembly, includes actuating mechanism and clamp plate mechanism, the clamp plate mechanism includes: the actuating pressure plate is driven by the driving mechanism to perform reciprocating linear motion along a set direction; the passive pressing plate moves linearly in a reciprocating way along with the actuating pressing plate and is used for jacking the reagent kit; the elastic adjusting component is arranged between the actuating pressure plate and the driven pressure plate and can adjust the distance between the actuating pressure plate and the driven pressure plate, and the elastic adjusting component comprises a connecting piece and an elastic piece; the actuating pressure plate and the connecting piece are arranged in a relatively movable mode, the elastic piece is always in a compression state, two ends of the elastic piece apply force to the actuating pressure plate and the driven pressure plate respectively, and the connecting piece is provided with a first limiting piece used for limiting the actuating pressure plate to be separated from the connecting piece; the minimum top pressure acting force required by the kit is Pmin, and the maximum top pressure acting force which can be borne by the kit is Pmax; the acting force exerted by the elastic piece on the passive pressing plate is F, when the passive pressing plate is not in contact with the reagent box, the F has a minimum value Fmin, when the passive pressing plate is pressed against the reagent box and the actuating pressing plate reaches the stroke end point of the reciprocating linear motion, the F has a maximum value Fmax, wherein Pmin < Fmin < Fmax < Pmax.
The invention has the following beneficial effects:
1. an actuating pressure plate in the pressure plate mechanism is driven to carry out reciprocating linear motion along a set direction, and the actuating pressure plate drives a driven pressure plate to carry out reciprocating linear motion along with the actuating pressure plate through an elastic adjusting component when carrying out reciprocating linear motion, so that the reagent box is jacked in the process of carrying out reciprocating linear motion on the driven pressure plate, and the puncture structure in the reagent box can puncture the sealing structure;
2. when the self-adaptive top pressure assembly provided by the invention is applied, the reciprocating travel of the pressing plate mechanism can be set according to the size of a small reagent kit caused by tolerance, so that the reagent kit with small size can be pressed in place;
3. for those reagent cartridges manufactured with a size larger than the designed size or those reagent cartridges with an increased size in the heating step, the increased size can be offset by the compression of the elastic member, so that the pressing member does not press the reagent cartridges excessively;
4. the acting force F exerted by the elastic piece on the passive pressing plate is the acting force exerted by the passive pressing plate on the reagent box, so that Fmin is more than Pmin, the reagent box can be always pressed by the passive pressing plate along a single direction in the process from the contact of the passive pressing plate and the reagent box to the complete pressing, and the reagent box cannot be bounced to the return stroke direction by overcoming the elastic force of the elastic piece due to the reverse acting force of the reagent box. Therefore, the pressing process of the passive pressing plate to the reagent box can be ensured to be rapid, powerful and direct in place, and the leakage is avoided.
Preferably, the actuating pressure plate is provided with a through hole along a set direction, the connecting piece penetrates through the through hole, the first limiting part is a limiting boss formed at one end of the connecting piece, a blocking structure matched with the limiting boss is arranged in the through hole, and the blocking structure is abutted against and pressed on the limiting boss under the action of the elastic piece. The connecting piece penetrates through the through hole, so that the actuating pressure plate can overcome the elastic force of the elastic piece to move relative to the connecting piece under the driving of the driving mechanism; and through setting up spacing boss, through spacing boss and the cooperation of blockking the structure, just can restrict actuating the clamp plate and break away from the connecting piece.
Preferably, the other end of the connecting piece is fixedly arranged on the passive pressure plate; or, the connecting piece and the driven pressing plate are arranged in a relatively movable manner along a set direction, a second limiting piece used for limiting the driven pressing plate to be separated from the connecting piece is arranged at the other end of the connecting piece, and the driven pressing plate is pressed against the second limiting piece under the pressing of the elastic piece. The connecting piece is fixedly arranged on the passive pressing plate or is pressed against the passive pressing plate through the elastic piece, and the connecting piece and the passive pressing plate can be fixed relatively.
Preferably, the connecting piece is externally sleeved with a guide ring matched with the connecting piece in a sliding manner, the guide ring is fixedly arranged on the actuating pressure plate, and the guide ring is provided with a blocking structure extending into the through hole. The actuating pressure plate is arranged outside the connecting piece in a sliding mode through the guide ring, namely the moving direction of the actuating pressure plate is limited through the guide ring, and the actuating pressure plate is guaranteed to move linearly. Therefore, the pressing plate mechanism can integrally move linearly, the jacking acting force of the passive pressing plate on the kit is prevented from deviating, and the puncture structure can puncture the sealing mechanism in the center.
Preferably, a self-adaptive distance is formed between the end face, facing the passive pressure plate, of the guide ring and the passive pressure plate, and the self-adaptive distance is a selected value between 1mm and 5 mm. The smaller self-adaptive distance is arranged, so that the integral lifting of the jacking assembly can be effectively reduced, and the requirement of product miniaturization is met.
Preferably, the guide ring is formed with an annular flange extending radially, and the annular flange is fixedly connected with the actuating pressure plate through bolts.
Preferably, the elastic part is a spring, the spring is sleeved outside the guide ring, one end of the spring is arranged on the annular flange, and the other end of the spring is arranged on the driven pressing plate. The spring has a good positioning effect, the situation that the spring is radially deviated in the compression process can be prevented, and the direction of the acting force of the spring on the driven pressing plate is kept unchanged.
Preferably, the guide ring is a linear bearing. The linear axial direction is selected for use, on one hand, the guiding effect can be well played, on the other hand, the friction between the linear axial direction and the connecting piece can be reduced, and the service life is prolonged.
Preferably, the jacking assembly further comprises a substrate, the driving mechanism is arranged on the substrate, a through hole for the pressing plate mechanism to perform reciprocating linear motion along a set direction is further formed in the substrate, and a guide post is arranged between the driven pressing plate and the substrate. The guide post is arranged between the substrate and the driven pressing plate, so that the motion of the driven pressing plate is guided, and the linear motion of the driven pressing plate is ensured. Therefore, the pressing plate mechanism can integrally move linearly, the jacking acting force of the passive pressing plate on the kit is prevented from deviating, and the puncture structure can puncture the sealing mechanism in the center.
Preferably, the driving mechanism comprises a driving motor and a cam transmission assembly, the cam transmission assembly comprises an eccentric cam and a transmission piece, and the eccentric cam is arranged on an output shaft of the driving motor and is driven to rotate around a set axis; one end of the transmission piece is arranged on the actuating pressure plate, the other end of the transmission piece is arranged on the eccentric cam, and the transmission piece drives the actuating pressure plate to perform reciprocating linear motion along a set direction under the driving of the eccentric cam. The pressing plate mechanism is driven by the driving motor and the cam transmission assembly, and the cam transmission assembly converts the rotary motion of the output shaft of the driving motor into the linear reciprocating motion of the pressing plate mechanism along the set direction, so that the driving mechanism does not need a large lifting movement space and occupies a small area. In addition, because the eccentric cam rotates around the set axis, the motion of the eccentric cam is stable, and therefore, under the driving of the eccentric cam, the stroke of the pressing plate mechanism driven by the transmission piece to perform reciprocating linear motion can be kept stable, and errors are reduced.
The invention also adopts the following technical scheme: a detection device for a kit comprises a shell, a support, an extraction amplification component, a photoelectric component and a self-adaptive top pressure component for the kit, wherein the support is arranged in the shell, and the extraction amplification component, the photoelectric component and the top pressure component are all arranged on the support. By adopting the self-adaptive jacking assembly, the detection device can ensure that the puncture structure in the reagent box stably and powerfully punctures the sealing structure and moves in place through jacking action, and ensures that the puncture structure has good conductivity after puncturing, so that the reagent is smoothly tapped.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Drawings
FIG. 1 is a schematic structural diagram of an adaptive top pressure assembly for a reagent cartridge according to an embodiment;
FIG. 2 is an exploded view of the pressing assembly of the embodiment;
FIG. 3 is a schematic structural view of a platen mechanism in the embodiment;
FIG. 4 is a schematic view of the cartridge pressed by the pressing member in the embodiment;
FIG. 5 is a schematic diagram of the reciprocating stroke provided to the ram assembly;
FIG. 6 is a cross-sectional view of the platen mechanism of FIG. 3;
FIG. 7 is a schematic structural view of a cam gear assembly in the embodiment;
FIG. 8 is an external view of a detecting device using the pressing member according to the embodiment;
fig. 9 is an internal schematic view of the detection apparatus of fig. 8.
Wherein: 1. the device comprises a driving mechanism, 10 driving motors, 11 eccentric cams, 110 first cams, 111 second cams, 112 driving grooves, 12 transmission pieces, 120 mounting shafts, 121 bearings, 2 actuating pressing plates, 20 perforation holes, 21 guide rings, 210 blocking structures, 211 annular flanges, 22 mounting holes, 3 driven pressing plates, 4 elastic adjusting members, 40 connecting pieces, 400 limiting bosses, 41 elastic pieces, 5 base plates, 50 through holes, 51 guide columns, 6 reagent boxes, 60 upper shells, 61 lower shells, 7 detection devices, 70 shells, 71 supports, 72 extraction amplification assemblies and 73 photoelectric assemblies.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the invention and are not to be construed as limiting the invention.
Embodiments of the present invention are described below with reference to the drawings.
Example (b): the embodiment provides an adaptive top pressing assembly for a reagent kit, which comprises a driving mechanism 1 and a pressing plate mechanism, wherein the pressing plate mechanism comprises an actuating pressing plate 2, a driven pressing plate 3 and an elastic adjusting member 4, and the actuating pressing plate 2 is driven by the driving mechanism 1 to perform reciprocating linear motion along a set direction, as shown in fig. 1 to 3. The setting direction mentioned here is determined according to the relative position of the pressing plate mechanism and the reagent kit, and specifically in this embodiment, the setting direction is a vertical direction, that is, the pressing plate mechanism performs a reciprocating linear motion along the vertical direction, and the reagent kit is placed below the pressing plate mechanism during use. In another embodiment, the set direction may be a horizontal direction, the platen mechanism may reciprocate linearly in the horizontal direction, and the reagent cartridge and the platen mechanism may be horizontally placed when used. The driven pressing plate 3 performs reciprocating linear motion along with the actuating pressing plate 2, wherein the following means that the actuating pressing plate 2 drives the driven pressing plate 3 to move together when performing the reciprocating linear motion, so that the driven pressing plate 3 presses the reagent kit 6. The actuating pressure plate 2 can drive the driven pressure plate 3 to perform reciprocating linear motion together, and the connecting is realized by an elastic adjusting member 4 arranged between the actuating pressure plate and the driven pressure plate, and specifically, the elastic adjusting member 4 comprises a connecting piece 40 and an elastic piece 41. Wherein the actuating platen 2 and the connecting member 40 are arranged to be relatively movable. The elastic member 41 always applies force to the actuating pressure plate 2 and the driven pressure plate 3 in a manner that the actuating pressure plate 2 and the driven pressure plate 3 have a tendency to be away from each other, that is, the elastic member 41 is always in a compressed state, and in the compressed state, both ends of the elastic member 41 always apply thrust to the actuating pressure plate 2 and the driven pressure plate 3 respectively. And the connecting member 40 is provided with a first stopper for restricting the disengagement of the actuating platen 2 from the connecting member 40. Therefore, when the reagent box is larger in size due to tolerance, the distance between the actuating pressing plate 2 and the driven pressing plate 3 is changed by compressing the elastic part 41 by the actuating pressing plate 2 in the jacking process, and the larger size of the reagent box is offset by changing the distance between the actuating pressing plate 2 and the driven pressing plate 3, namely, the reagent boxes with different sizes are adaptive. The top pressure of the kit is ensured to be in place, and the excessive top pressure is avoided.
Referring to fig. 4 and 5, the stroke setting of the platen mechanism in the pressing assembly is described as follows: this roof pressure subassembly is used for the roof pressure kit to realize switching on of the inside test tube of kit and extraction pond, and the structure of this kind of kit is as shown in fig. 4, and kit 6 includes epitheca 60 and inferior valve 61, is provided with the puncture structure in the epitheca 60, is provided with the reagent pipe in the inferior valve 61, and reagent pipe mouth of pipe department is provided with the seal membrane. The above structure of such a kit belongs to the prior art and is not described herein in detail. As shown in FIG. 5, the upper casing 60 is moved downward by a distance L relative to the lower casing 61 to puncture the sealing membrane through the puncture structure and to allow the puncture structure to extend into a suitable position in the reagent tube (which ensures smooth reagent pumping operation). The platen mechanism is shown in two different positions in fig. 5, the platen mechanism being at the highest point of the reciprocating linear motion and the platen mechanism moving down to a position just in contact with the reagent vessel. The stroke of the reciprocating linear motion of the actuating pressure plate 2 comprises a vertical downward going stroke and a vertical upward returning stroke, the stroke amount of a single going stroke or a single returning stroke of the actuating pressure plate 2 is set to be H, and the stroke H of the actuating pressure plate 2 needs to be preset according to the pressing distance of the reagent kit 6 during design and manufacture. When the passive pressure plate 3 is located at the highest position, the distance between the lower surface of the passive pressure plate 3 and the upper surface of the upper case 60 is set to be P. In an ideal state, H may be set to H = L + P. However, in consideration of the manufacturing tolerance, the expansion due to the temperature change during the detection, and the like as described above, H is designed as H = L + P + Δ H in the present embodiment, where Δ H is the tolerance that the reagent cartridge 6 inevitably undergoes during the manufacturing process, and can be obtained by performing measurement on the reagent cartridge 6 that is manufactured in mass production.
That is, when H is preset, the size of the reagent kit is set to be smaller due to tolerance, so that the reagent kit with the smaller size can be pressed in place. For those cartridges manufactured with a size larger than the design size or those cartridges that increase in size during the heating step, the above-mentioned dimensional change can be counteracted by the compression of the elastic member. Therefore, the top pressing component provided by the embodiment can be used for pressing the reagent box in place without excessive top pressing. In contrast, in the related art, the pressing plate mechanism without the self-adaptive capability generally designs H = L + P, and can only achieve a better pressing effect on the reagent kit meeting the design size requirement. It will not bear well against the reagent vessels of smaller dimensions due to tolerances during manufacture, will bear too much against the reagent vessels of larger dimensions due to tolerances during manufacture, and correspondingly will bear too much against the reagent vessels of increased dimensions due to heating during the detection process.
As described above, the set direction in this embodiment is a vertical direction, and therefore when the pressing member is applied, the reagent cartridge 6 is placed below the platen mechanism, and the platen mechanism moves downward to contact the reagent cartridge 6 and presses the reagent cartridge 6. So that the puncture structure inside the reagent kit 6 punctures the sealing structure and conducts the reagent tube with the extraction pool. And then the top pressure component is used for ensuring the stability of the reagent kit through the top pressure action of the reagent kit, and the pressing plate mechanism moves upwards to be separated from the reagent kit 6 after the steps of mixing, amplifying and detecting are finished. Then, the pressing operation is performed after the next reagent cartridge 6 is placed.
As mentioned above, the elastic member in the pressing assembly provided in this embodiment is always in a compressed state, and the pre-pressing degree of the elastic member in this embodiment is specifically designed: the minimum pressing force required by the reagent kit is set as Pmin, the maximum pressing force that the reagent kit can bear is set as Pmax, and the force exerted by the elastic member 41 on the passive pressing plate 3 is set as F (the force F can represent the degree of the compression of the elastic member 41). F has a minimum value Fmin when the passive platen 3 is not in contact with the kit; when the passive pressure plate 3 presses the reagent box and the actuating pressure plate reaches the stroke end point of the reciprocating linear motion (namely, the lowest point), F has a maximum value Fmax, wherein Pmin < Fmin < Fmax < Pmax.
The puncture structure needs to ensure that the puncture is rapid and powerful in the whole puncture process of puncturing the sealing membrane and extending into the proper position in the reagent tube, but the puncture cannot be interrupted or blocked, and if the interruption or the blocking occurs, the possibility of leakage of the test solution (the puncture cannot be rapidly punctured for realizing the sealing) can occur between the puncture structure and the sealing membrane at the moment of interruption or blocking. The acting force F exerted by the elastic member 41 on the passive pressing plate 3 is the acting force exerted by the passive pressing plate 3 on the reagent cartridge 6, and in this embodiment, fmin is set to be greater than Pmin, so that it can be ensured that the reagent cartridge 6 is always pressed by the passive pressing plate 3 along a single direction in the process from the time when the passive pressing plate 3 is in contact with the reagent cartridge 6 to the time when the passive pressing plate is completely pressed in place, and the reagent cartridge 6 cannot be bounced in the return direction by overcoming the elastic force of the elastic member 41 due to the reverse acting force of the reagent cartridge 6. Therefore, the pressing process of the passive pressing plate 3 to the reagent box 6 can be ensured to be rapid, powerful and direct in place, and the leakage is avoided. It can be understood that, after the pressing assembly is pressed in place, if the reagent kit expands due to heating in the detection process, the expanded reagent kit can apply a large acting force to the passive pressing plate, and at the moment, the elastic member is compressed to enable the passive pressing plate to move upwards, so that the pressing assembly can adapt to the size change.
Referring to fig. 6, the actuating platen 2 in this embodiment is provided with a through hole 20 along a set direction, the connecting member 40 is disposed through the through hole 20, the first limiting member is a limiting boss 400 formed at one end of the connecting member 40, a blocking structure 210 adapted to the limiting boss 400 is disposed in the through hole 20, and the blocking structure 210 is pressed against the limiting boss 400 under the action of the elastic member 41. The connecting piece 40 is arranged through the through hole 20, so that the actuating pressure plate 2 can move relative to the connecting piece 40 against the elastic force of the elastic piece 41 under the driving of the driving mechanism 1; and the separation of the actuating pressure plate 2 from the connecting member 40 can be restricted by providing the restricting projection 400, and by engaging the restricting projection 400 with the blocking structure 210. The limiting boss 400 is integrally formed with the connecting member 40 in this embodiment, and it should be understood that in other embodiments, the first limiting member may also be fixedly mounted on the connecting member 40 by a fixed connection manner, for example, the first limiting member is screwed onto the connecting member 40, or is inserted into the connecting member 40 with a tight fit. The shape of the first limiting member is not limited to the cylindrical shape shown in the embodiment, and may also be a rectangular shape or other shapes, and the first limiting member can cooperate with the blocking structure 210 to perform a limiting function.
In addition, in the present embodiment, the other end of the connecting member 40 is fixedly disposed on the passive pressure plate 3, so as to realize the relative fixation of the connecting member 40 and the passive pressure plate 3. Specifically, a stepped hole is formed in the passive pressing plate 3, a bolt penetrates through the stepped hole, a threaded hole matched with the bolt is formed in the end portion of the connecting piece 40, and the passive pressing plate 3 and the connecting piece 40 are fixedly mounted through threaded connection. It can be understood that the connecting member 40 and the passive pressure plate 3 can be fixedly connected by means of integral molding, tight-fit insertion, and the like. Of course, one end of the connecting member 40 is provided with the limiting boss 400, and the other end of the connecting member 40 is provided on the passive pressing plate 3, so that at least one end of the two ends of the connecting member is detachably connected to facilitate the assembly of the actuating pressing plate 2. In other embodiments, the relative fixing arrangement of the connecting piece and the passive pressure plate can also be realized by adopting the following structure: the connecting piece and the driven pressing plate are arranged in a relatively movable mode along the set direction, the other end of the connecting piece is provided with a second limiting piece used for limiting the driven pressing plate to be separated from the connecting piece, and the driven pressing plate is pressed on the second limiting piece under the pressing of the elastic piece. The passive pressing plate can not move relative to the connecting piece through the abutting action of the elastic piece on the passive pressing plate.
In this embodiment, a guide ring 21 adapted to the connecting member 40 is slidably sleeved outside the connecting member 40, the guide ring 21 is fixedly disposed on the actuating pressure plate 2, specifically, an annular flange 211 is formed on the guide ring 21 in a radially extending manner, and the annular flange 211 is fixedly connected to the actuating pressure plate 2 by bolts. The guide ring 21 has a blocking structure 210 extending into the through hole 20, so that the actuating platen 2 is slidably disposed outside the connecting member 40 through the guide ring 21, i.e., the moving direction of the actuating platen 2 is limited by the guide ring 21, and the actuating platen is ensured to perform linear movement. The whole pressing plate mechanism moves linearly, so that the pressing action force of the passive pressing plate 3 on the top of the kit is prevented from deviating, and the puncture structure can puncture the sealing mechanism in the middle. Further, in the present embodiment, the elastic member 41 is a spring, the spring is sleeved outside the guide ring 21, one end of the spring is disposed on the annular flange 211, and the other end of the spring is disposed on the passive pressure plate 3. Have good positioning action to the spring like this, can prevent that the spring from taking place the condition of radial skew at the in-process of compressing, and then guarantee that the direction of action of spring to passive clamp plate 3 keeps unchangeable. In other embodiments, the elastic member 41 may be an elastic member 41 such as a spring plate, and the elastic member 41 may be disposed between the actuation pressing plate 2 and the passive pressing plate 3.
The guide ring 21 in this embodiment is a linear bearing, which can perform a good guiding function on one hand, and can reduce friction with the connecting member 40 and prolong the service life on the other hand. The linear bearing is manufactured directly into the annular flange 211, and the size of the linear bearing is adapted to the size of the through hole 20, that is, the upper section of the linear bearing can extend into the through hole 20, and the upper section of the linear bearing extending into the through hole 20 is the blocking structure 210.
The jacking assembly further comprises a substrate 5, the driving mechanism 1 is arranged on the substrate 5, a through hole 50 for the pressing plate mechanism to perform reciprocating linear motion along a set direction is further formed in the substrate 5, and a guide post 51 is arranged between the driven pressing plate 3 and the substrate 5. The guide post 51 is arranged between the substrate 5 and the passive pressing plate 3 to guide the motion of the passive pressing plate 3 and ensure the linear motion of the passive pressing plate. Therefore, the pressing plate mechanism can integrally move linearly, the jacking acting force of the passive pressing plate 3 on the reagent kit 6 is prevented from deviating, and the puncture structure can puncture the sealing mechanism in the center.
In the present embodiment, the end surface of the guide ring 21 facing the passive pressing plate 3 has a self-adaptive distance with the passive pressing plate 3, and in order to make the entire pressing assembly have the advantages of miniaturization and portability, the self-adaptive distance is set to be 2mm in the present embodiment. The inventor finds through experimental study that the size change caused by tolerance, thermal expansion and the like generally does not exceed 0.5mm, and accordingly the adaptive spacing can be set to a selected value between 1mm and 5mm, so that the adaptive spacing can be sufficiently adjusted according to the size of the kit, and meanwhile, the smaller adaptive spacing can be set to effectively reduce the overall size of the jacking assembly so as to meet the requirement of product miniaturization.
As shown in fig. 1 and 7, the driving mechanism 1 in the present embodiment includes a driving motor 10 and a cam transmission assembly including an eccentric cam 11 and a transmission member 12, the eccentric cam 11 being provided on an output shaft of the driving motor 10 and being driven to rotate about a set axis; one end of the transmission member 12 is disposed on the actuating pressing plate 2, the other end of the transmission member 12 is disposed on the eccentric cam 11, and the transmission member 12 drives the actuating pressing plate 2 to perform reciprocating linear motion along a set direction under the driving of the eccentric cam 11. The pressing plate mechanism is driven by the driving motor 10 and the cam transmission assembly, and the cam transmission assembly converts the rotary motion of the output shaft of the driving motor 10 into the linear reciprocating motion of the pressing plate mechanism along the set direction, so that the driving mechanism 1 does not need a large lifting moving space and occupies a small area. In addition, because the eccentric cam 11 rotates around the set axis, the motion of the eccentric cam is stable, and therefore, the stroke of the pressing plate mechanism driven by the transmission piece 12 to perform reciprocating linear motion can be kept stable, and errors are reduced.
Specifically, in the present embodiment, the eccentric cam 11 includes a first cam 110 and a second cam 111, the first cam 110 is provided with a groove, the second cam 111 is formed in the groove, and a driving groove 112 is formed by the first cam 110 and the second cam 111 in a matching manner. The transmission member 12 includes a mounting shaft 120 and a bearing 121, wherein one end of the mounting shaft 120 is fixedly inserted into the actuating platen 2, and specifically, a mounting hole 22 (shown in fig. 3) adapted to the mounting shaft 120 is provided on the actuating platen 2; the other end of the mounting shaft 120 is in interference fit with the inner race of the bearing 121, while the outer race of the bearing 121 is in rolling fit with the inner wall of the drive groove 112. Thus, when the driving motor 10 rotates, the eccentric cam 11 performs eccentric rotation motion around the axis of the output shaft of the driving motor 10, in the process, the inner wall of the driving groove 112 is in rolling contact with the outer ring of the bearing 121, and generates pressing effect on the bearing 121, and the bearing 121 drives the actuating pressure plate 2 to perform reciprocating linear motion. The advantage of such a driving structure is that, since the outer ring of the bearing 121 and the inner wall of the driving groove 112 are in rolling fit, the wear of the two is very small, thereby avoiding the change of the stroke of the linear reciprocating motion of the actuation pressure plate 2 due to the wear. This further ensures that the pressing plate mechanism presses the reagent cartridge 6 in place without excessive pressing.
When a sample in the reagent kit is detected, the pressing component provided in this embodiment serves as a unit in the detection device to press the reagent kit, and fig. 8 and 9 show schematic structural diagrams of the detection device using the pressing component. The detection device 7 comprises a housing 70, a bracket 71, an extraction and amplification assembly 72, a photoelectric assembly 73 and the top pressure assembly, wherein the bracket 71 is arranged in the housing 70, and the extraction and amplification assembly 72, the photoelectric assembly 73 and the top pressure assembly are all arranged on the bracket 71. Due to the adoption of the self-adaptive jacking assembly, the detection device 7 can ensure that the puncture structure in the kit 6 can stably and powerfully puncture the sealing structure and move in place through jacking action, and ensures that the puncture structure has good conductivity after puncture, so that the reagent is smoothly pumped.
When detection is carried out, the jacking assembly is translated through the sliding mechanism to avoid the position, then the reagent box is placed on the set station, the jacking assembly is translated to the original position, the pressing plate mechanism is located above the reagent box, and then the pressing plate mechanism is driven to jack the reagent box by controlling the driving motor. And after the puncture is finished, the reagent tube is communicated with the extraction pool, and then the steps of mixing, amplifying, detecting and the like are carried out, wherein in the process, the steps of mixing and amplifying are finished by the extraction and amplification component, and the step of detecting is finished by the photoelectric component.
In the present invention, unless otherwise explicitly stated or limited by the relevant description or limitation, the terms "mounted," "connected," and "fixed" in the embodiments are to be understood in a broad sense, for example, the connection may be a fixed connection, a detachable connection, or an integrated connection, and it may be understood that the connection may also be a mechanical connection, an electrical connection, etc.; of course, they may be directly connected or indirectly connected through intervening media, or they may be interconnected within one another or in an interactive relationship. Those of ordinary skill in the art will understand the specific meaning of the above terms in the present invention according to their specific implementation.
Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.
Claims (11)
1. The utility model provides a kit is with self-adaptation roof pressure subassembly, includes actuating mechanism (1) and clamp plate mechanism, its characterized in that, clamp plate mechanism includes:
an actuating platen (2) driven by the driving mechanism (1) to perform reciprocating linear motion along a set direction;
a passive pressing plate (3) which moves linearly and reciprocally along with the actuating pressing plate (2) and is used for pressing the reagent box; and the number of the first and second groups,
the elastic adjusting component (4) is arranged between the actuating pressure plate (2) and the driven pressure plate (3) and can adjust the distance between the actuating pressure plate and the driven pressure plate, and the elastic adjusting component (4) comprises a connecting piece (40) and an elastic piece (41);
the actuating pressure plate (2) and the connecting piece (40) are arranged in a relatively movable manner, the elastic piece (41) is always in a compression state, two ends of the elastic piece apply force to the actuating pressure plate (2) and the driven pressure plate (3) respectively, and the connecting piece (40) is provided with a first limiting piece for limiting the actuating pressure plate (2) to be separated from the connecting piece (40);
the minimum top pressure acting force required by the kit is Pmin, and the maximum top pressure acting force which can be borne by the kit is Pmax;
the acting force exerted by the elastic piece (41) on the passive pressing plate (3) is F, when the passive pressing plate (3) is not in contact with the reagent box, the F has a minimum value Fmin, when the passive pressing plate (3) presses the reagent box and the actuating pressing plate (2) reaches the stroke end point of the reciprocating linear motion, the F has a maximum value Fmax, wherein Pmin < Fmin < Fmax < Pmax.
2. The adaptive top pressure assembly for reagent cartridges according to claim 1, wherein the actuating platen (2) is provided with a through hole (20) along a set direction, the connecting member (40) is disposed through the through hole (20), the first limiting member is a limiting boss (400) formed on one end of the connecting member (40), a blocking structure (210) adapted to the limiting boss (400) is disposed in the through hole (20), and the blocking structure (210) is pressed against the limiting boss (400) by the elastic member (41).
3. The adaptive top pressure assembly for reagent cartridges according to claim 2, wherein the other end of the connecting member (40) is fixedly disposed on the passive pressure plate (3); or the like, or a combination thereof,
the connecting piece (40) and the driven pressing plate (3) are arranged in a relatively movable mode along a set direction, a second limiting piece used for limiting the driven pressing plate (3) to be separated from the connecting piece (40) is arranged at the other end of the connecting piece (40), and the driven pressing plate (3) is pressed against the second limiting piece under the pressing of the elastic piece (41).
4. Adaptive top pressure assembly for reagent cartridges as in claim 2, characterized in that the connecting piece (40) is slidably sleeved with a guide ring (21) adapted to the connecting piece (40), the guide ring (21) is fixedly arranged on the actuating pressure plate (2), and the guide ring (21) has a blocking structure (210) extending into the through hole (20).
5. The adaptive top pressure assembly for reagent cartridges according to claim 4, characterized in that the end surface of the guide ring (21) facing the passive pressure plate (3) has an adaptive spacing from the passive pressure plate (3), the adaptive spacing being a selected value between 1mm and 5 mm.
6. Adaptive top pressure assembly for reagent cartridges according to claim 4, characterized in that the guide ring (21) has an annular flange (211) extending radially, and the annular flange (211) is fixedly connected with the actuating pressure plate (2) by bolts.
7. The adaptive top pressure assembly for reagent cartridges according to claim 6, wherein the elastic member (41) is a spring, the spring is sleeved outside the guide ring (21), one end of the spring is disposed on the annular flange (211), and the other end of the spring is disposed on the passive pressure plate (3).
8. Adaptive top pressure assembly for reagent cartridges according to claim 4, characterized in that the guide ring (21) is a linear bearing.
9. The adaptive top pressure assembly for reagent kit according to claim 1, further comprising a base plate (5), wherein the driving mechanism (1) is disposed on the base plate (5), the base plate (5) is further provided with a through hole (50) for the pressing plate mechanism to perform a reciprocating linear motion along a set direction, and a guide column (51) is disposed between the passive pressing plate (3) and the base plate (5).
10. The adaptive top pressure assembly for reagent kit according to claim 1, wherein the driving mechanism (1) comprises a driving motor (10) and a cam transmission assembly, the cam transmission assembly comprises an eccentric cam (11) and a transmission member (12), the eccentric cam (11) is arranged on an output shaft of the driving motor (10) and is driven to rotate around a set axis;
one end of the transmission piece (12) is arranged on the actuating pressing plate (2), the other end of the transmission piece (12) is arranged on the eccentric cam (11), and the transmission piece (12) drives the actuating pressing plate (2) to perform reciprocating linear motion along a set direction under the driving of the eccentric cam (11).
11. A detection device for a kit, comprising a housing (70), a holder (71), an extraction amplification module (72), a photoelectric module (73), and the adaptive roof pressure module for a kit according to any one of claims 1 to 10, wherein the holder (71) is disposed in the housing (70), and the extraction amplification module (72), the photoelectric module (73), and the roof pressure module are disposed on the holder (71).
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| CN202211145853.5A CN115651819B (en) | 2022-09-20 | 2022-09-20 | Self-adaptive jacking component for kit and detection device |
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Citations (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10277666A (en) * | 1997-04-08 | 1998-10-20 | Fuji Heavy Ind Ltd | Aligning device for piercing jig |
| EP2070691A2 (en) * | 2007-12-13 | 2009-06-17 | Mr. Robert John Lockwood | Waste Compactor |
| CN103725602A (en) * | 2012-10-16 | 2014-04-16 | 常州福生生物技术有限公司 | Thermal cover plate used in nucleic acid amplification detector |
| US20160043053A1 (en) * | 2012-12-21 | 2016-02-11 | Shinkawa Ltd. | Flip chip bonder and method of correcting flatness and deformation amount of bonding stage |
| CN107603859A (en) * | 2017-11-08 | 2018-01-19 | 西安天隆科技有限公司 | Fully automatic integral nucleic acid extraction, amplification and detecting system |
| US20190348308A1 (en) * | 2018-05-09 | 2019-11-14 | Semigear, Inc. | Substrate pressing module, substrate pressing method, substrate treating apparatus including the substrate treating module, and the substrate treating method |
| CN111187713A (en) * | 2020-02-21 | 2020-05-22 | 厦门大学 | Micro-fluidic chip's puncture device and micro-fluidic chip detecting system |
| CN112173303A (en) * | 2020-09-27 | 2021-01-05 | 郑州佐爵生物科技有限公司 | A presser device that is used for biochip kit of different specifications |
| CN212504899U (en) * | 2020-05-15 | 2021-02-09 | 北京百康芯生物科技有限公司 | Full-automatic nucleic acid detection and analysis system |
| CN212532957U (en) * | 2020-05-20 | 2021-02-12 | 扬州市董氏特种水产有限公司 | Rapid diagnosis kit for infectious spleen and kidney necrosis virus of mandarin fish |
| CN112613588A (en) * | 2021-01-05 | 2021-04-06 | 南京溪则宏贸易有限公司 | Intelligent card chip abrasionproof decreases safety arrangement |
| CN112770529A (en) * | 2020-12-17 | 2021-05-07 | 吕贤虾 | Automatic hot melting device of double-shaft chip packaging |
| CN112795464A (en) * | 2021-01-29 | 2021-05-14 | 中国科学院苏州生物医学工程技术研究所 | Droplet generating device |
| CN214622273U (en) * | 2021-04-12 | 2021-11-05 | 沈阳医陆生物科技有限公司 | Shell breaking and sampling device of chemiluminescence tester |
| CN215050301U (en) * | 2020-10-30 | 2021-12-07 | 中元汇吉生物技术股份有限公司 | Nucleic acid detection analyzer |
| CN215415750U (en) * | 2021-06-23 | 2022-01-04 | 成都思科瑞微电子股份有限公司 | Chip module testing device |
| CN114433263A (en) * | 2022-02-08 | 2022-05-06 | 广东永诺医疗科技有限公司 | Liquid-transfering, microdroplet generation and film sealing system |
| CN114784618A (en) * | 2022-06-22 | 2022-07-22 | 度亘激光技术（苏州）有限公司 | Chip packaging method, negative plate and laser |
| CN115651801A (en) * | 2022-09-20 | 2023-01-31 | 杭州准芯生物技术有限公司 | A roof pressure subassembly and detecting instrument for kit |
-
2022
- 2022-09-20 CN CN202211145853.5A patent/CN115651819B/en active Active
Patent Citations (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10277666A (en) * | 1997-04-08 | 1998-10-20 | Fuji Heavy Ind Ltd | Aligning device for piercing jig |
| EP2070691A2 (en) * | 2007-12-13 | 2009-06-17 | Mr. Robert John Lockwood | Waste Compactor |
| CN103725602A (en) * | 2012-10-16 | 2014-04-16 | 常州福生生物技术有限公司 | Thermal cover plate used in nucleic acid amplification detector |
| US20160043053A1 (en) * | 2012-12-21 | 2016-02-11 | Shinkawa Ltd. | Flip chip bonder and method of correcting flatness and deformation amount of bonding stage |
| CN107603859A (en) * | 2017-11-08 | 2018-01-19 | 西安天隆科技有限公司 | Fully automatic integral nucleic acid extraction, amplification and detecting system |
| US20190348308A1 (en) * | 2018-05-09 | 2019-11-14 | Semigear, Inc. | Substrate pressing module, substrate pressing method, substrate treating apparatus including the substrate treating module, and the substrate treating method |
| CN111187713A (en) * | 2020-02-21 | 2020-05-22 | 厦门大学 | Micro-fluidic chip's puncture device and micro-fluidic chip detecting system |
| CN212504899U (en) * | 2020-05-15 | 2021-02-09 | 北京百康芯生物科技有限公司 | Full-automatic nucleic acid detection and analysis system |
| CN212532957U (en) * | 2020-05-20 | 2021-02-12 | 扬州市董氏特种水产有限公司 | Rapid diagnosis kit for infectious spleen and kidney necrosis virus of mandarin fish |
| CN112173303A (en) * | 2020-09-27 | 2021-01-05 | 郑州佐爵生物科技有限公司 | A presser device that is used for biochip kit of different specifications |
| CN215050301U (en) * | 2020-10-30 | 2021-12-07 | 中元汇吉生物技术股份有限公司 | Nucleic acid detection analyzer |
| CN112770529A (en) * | 2020-12-17 | 2021-05-07 | 吕贤虾 | Automatic hot melting device of double-shaft chip packaging |
| CN112613588A (en) * | 2021-01-05 | 2021-04-06 | 南京溪则宏贸易有限公司 | Intelligent card chip abrasionproof decreases safety arrangement |
| CN112795464A (en) * | 2021-01-29 | 2021-05-14 | 中国科学院苏州生物医学工程技术研究所 | Droplet generating device |
| CN214622273U (en) * | 2021-04-12 | 2021-11-05 | 沈阳医陆生物科技有限公司 | Shell breaking and sampling device of chemiluminescence tester |
| CN215415750U (en) * | 2021-06-23 | 2022-01-04 | 成都思科瑞微电子股份有限公司 | Chip module testing device |
| CN114433263A (en) * | 2022-02-08 | 2022-05-06 | 广东永诺医疗科技有限公司 | Liquid-transfering, microdroplet generation and film sealing system |
| CN114784618A (en) * | 2022-06-22 | 2022-07-22 | 度亘激光技术（苏州）有限公司 | Chip packaging method, negative plate and laser |
| CN115651801A (en) * | 2022-09-20 | 2023-01-31 | 杭州准芯生物技术有限公司 | A roof pressure subassembly and detecting instrument for kit |
Non-Patent Citations (2)
| Title |
|---|
| KORTMANN H等: "A rapid, reliable, and automatable lab-on-a-chip interfaced", LAB ON A CHIP, vol. 9, no. 10, 3 March 2009 (2009-03-03), pages 1455 - 1460, XP007920947, DOI: 10.1039/b820183h * |
| 郑建勇，周家春，秦建明: "智能弹簧检测仪研制", 自动化与仪器仪表, no. 03, 25 March 1994 (1994-03-25), pages 39 - 42 * |
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Denomination of invention: An adaptive top pressure component and detection device for a reagent kit Granted publication date: 20240517 Pledgee: Hangzhou branch of Bank of Nanjing Co.,Ltd. Pledgor: HANGZHOU ZHUNXIN BIOTECHNOLOGY Co.,Ltd. Registration number: Y2024980039231 |
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