CN115651819B - Self-adaptive jacking component for kit and detection device - Google Patents
Self-adaptive jacking component for kit and detection device Download PDFInfo
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Abstract
The invention discloses a self-adaptive jacking component for a kit, which comprises a driving mechanism and a pressing plate mechanism, wherein the pressing plate mechanism comprises: an actuation platen that performs a reciprocating linear motion in a set direction; a passive platen for pressing the kit; an elastic regulating member including a connecting piece and an elastic piece and disposed between the actuation platen and the driven platen; the actuating pressing plate and the connecting piece can be arranged in a relatively movable way; the elastic piece always applies force to the actuation pressing plate 5 and the driven pressing plate, and the connecting piece is provided with a first limiting piece for limiting the actuation pressing plate; minimum jacking acting force Pmin and maximum jacking acting force Pmax required by the kit; the acting force of the elastic piece on the passive plate has a minimum value Fmin and a maximum value Fmax, and Pmin is smaller than Fmin and Fmax is smaller than Pmax. The detection device comprising the jacking component is also disclosed. When the method is used for jacking the kit, the method can adapt to the dimensional change of the kit caused by the influence of tolerance and temperature, and can jack the kit in place without excessive jacking.
Description
Technical Field
The invention belongs to the field of biological detection devices, and particularly relates to a self-adaptive jacking component for a kit and a detection device.
Background
PCR is a short term for polymerase chain reaction, and is also called cell-free molecular cloning or specific DNA sequence in vitro primer directed enzymatic amplification technology in the field of biological detection, and is a method for in vitro enzymatic synthesis of specific DNA fragments. The method comprises the steps of high-temperature denaturation, low-temperature annealing, temperature-adaptive extension and the like to form a cycle, and the cycle is circularly carried out, so that target DNA can be rapidly amplified. The method has the characteristics of strong specificity, high sensitivity, simple and convenient operation, time saving and the like, and can be used for basic researches such as gene separation cloning, nucleic acid sequence analysis and the like, and can also be used for diagnosing diseases and any places with DNA and RNA. At present, the amplification technology is widely applied to nucleic acid detection. In actual operation, the problem of cross contamination of amplified products is easy to occur, namely, negative samples are contaminated with positive substances, false positive reaction is caused, false detection reports can be obtained, and great trouble and trouble are caused for detected personnel. In order to solve the above problems, a special kit is designed in the related art, which comprises two parts that can slide relatively, a reagent tube containing an amplification product is sealed with an elastic member, and is put into one of the parts of the kit. In the detection, the sealing structure on the reagent tube is pierced by the piercing structure arranged on the other part of the kit, so that cross contamination of the nucleic acid amplification products can be prevented.
In order to enable the two parts of the kit to slide relatively to realize penetration, a jacking assembly is required to jack the kit, and the jacking assembly in the related art generally uses a linear driving mechanism to drive a pressing plate to do reciprocating linear motion so as to jack the kit. In order to ensure the puncture effect, the kit has very high requirements on the jacking degree: the insufficient jacking degree can easily cause the puncture structure not to completely enter, and the puncture structure cannot be matched with the elastic piece to realize sealing, so that leakage is caused; excessive jacking is easy to cause the elastic piece to be excessively extruded and deformed, so that liquid in the pipe cannot be whipped. In the related art, the reciprocating linear motion stroke of the pressing plate is preset, dimensional tolerance is unavoidable in the manufacturing process of the kit, and dimensional change is generated due to temperature change under the influence of heating environment in the detection process. Therefore, when the kit is jacked, part of the kit can be jacked out of place or excessively jacked, and the detection result is greatly influenced.
Disclosure of Invention
The present invention aims to solve at least one of the technical problems in the related art to a certain extent, and for this purpose, the present invention adopts the following technical scheme: the utility model provides a self-adaptation roof pressure subassembly for kit, includes actuating mechanism and clamp plate mechanism, clamp plate mechanism includes: an actuation platen driven by the driving mechanism to reciprocate in a set direction; a passive platen which performs a reciprocating linear motion following the actuation platen and is used for pushing the reagent cartridge; and an elastic regulating member provided between the actuation platen and the driven platen and capable of regulating a gap therebetween, the elastic regulating member including a connector and an elastic piece; the elastic piece is always in a compressed state, two ends of the elastic piece respectively apply force to the actuating pressing plate and the driven pressing plate, and the connecting piece is provided with a first limiting piece used for limiting the actuating pressing plate to be separated from the connecting piece; the minimum jacking acting force required by the kit is Pmin, and the maximum jacking acting force bearable by the kit is Pmax; the acting force applied by the elastic piece to the passive plate is F, when the passive plate is not in contact with the kit, the F has a minimum value Fmin, and when the passive plate presses the kit and the actuating pressing plate reaches the stroke end of the reciprocating linear motion, the F has a maximum value Fmax, wherein Pmin < Fmin < Fmax < Pmax.
The invention has the following beneficial effects:
1. The actuating pressing plate in the pressing plate mechanism is driven to reciprocate in a set direction, and the actuating pressing plate drives the driven pressing plate to reciprocate in a linear manner through the elastic adjusting component when reciprocating, so that the reagent box is propped in the process of reciprocating the driven pressing plate, and the puncture of the sealing structure by the puncture structure in the reagent box is realized;
2. When the self-adaptive jacking component provided by the invention is applied, the reciprocating travel of the pressing plate mechanism can be set according to the size of the smaller reagent kit caused by tolerance, so that jacking in place of the smaller reagent kit can be ensured;
3. For those kits with manufacturing sizes larger than the design size, or kits with increased sizes in the heating step, the increased sizes can be counteracted by compression of the elastic piece, so that the jacking component cannot excessively jack the kits;
4. The acting force F applied by the elastic piece to the passive plate is the acting force applied by the passive plate to the kit, so Fmin is larger than Pmin, and the passive plate can be enabled to be always propped against the kit in a single direction in the process from the contact with the kit until the complete propping is in place, and the elastic force of the elastic piece can not be overcome to be sprung towards the return direction due to the reverse acting force of the kit. Therefore, the rapid, powerful and direct in-place jacking process of the kit by the passive pressure plate can be ensured, and the occurrence of leakage is avoided.
Preferably, the actuation pressing plate is provided with a perforation along a set direction, the connecting piece passes through the perforation, the first limiting piece is a limiting boss formed on one end of the connecting piece, a blocking structure matched with the limiting boss is arranged in the perforation, and the blocking structure is propped against the limiting boss under the action of the elastic piece. The connecting piece passes through the perforation, so that the actuating pressing plate can move relative to the connecting piece against the elastic force of the elastic piece under the drive of the driving mechanism; and through setting up spacing boss, through spacing boss and the cooperation of blocking structure, just can restrict to actuate the clamp plate and break away from the connecting piece.
Preferably, the other end of the connecting piece is fixedly arranged on the passive pressing plate; or the connecting piece and the driven pressing plate are arranged in a relatively movable manner along the set direction, the other end of the connecting piece is provided with a second limiting piece used for limiting the driven pressing plate to be separated from the connecting piece, and the driven pressing plate is pressed against the second limiting piece under the action of the elastic piece. The connecting piece is fixedly arranged on the passive dynamic pressure plate or is propped against the passive dynamic pressure plate through the elastic piece, and the relative fixation of the connecting piece and the passive dynamic pressure plate can be realized.
Preferably, the connecting piece is provided with a guide ring matched with the connecting piece in a sliding manner, the guide ring is fixedly arranged on the actuating pressing plate, and the guide ring is provided with a blocking structure extending into the through hole. The actuating pressing plate is arranged outside the connecting piece in a sliding way through the guide ring, namely, the moving direction of the actuating pressing plate is limited through the guide ring, so that the actuating pressing plate is ensured to perform linear movement. Therefore, the whole pressing plate mechanism can linearly move, the pushing acting force of the driven pressing plate on the kit is guaranteed not to deviate, and the puncture structure is guaranteed to puncture the sealing mechanism in the middle.
Preferably, an adaptive distance is arranged between the end face, facing the passive pressing plate, of the guide ring and the passive pressing plate, and the adaptive distance is a selected value between 1mm and 5mm. The smaller self-adaptive distance is arranged, so that the integral mention of the jacking assembly can be effectively reduced, and the requirement of miniaturization of products is met.
Preferably, the guide ring is formed with an annular flange extending in the radial direction, and the annular flange is fixedly connected with the actuation pressing plate through bolts.
Preferably, the elastic piece is a spring, the spring is sleeved outside the guide ring, one end of the spring is arranged on the annular flange, and the other end of the spring is arranged on the passive pressing plate. Therefore, the spring positioning device has a good positioning effect on the spring, the spring can be prevented from radial deflection in the compressed process, and the direction of the acting force of the spring on the passive plate is kept unchanged.
Preferably, the guide ring is a linear bearing. The linear axial direction is selected to play a good role in guiding, friction between the linear axial direction and the connecting piece can be reduced, and the service life of the linear axial direction is prolonged.
Preferably, the jacking assembly further comprises a substrate, the driving mechanism is arranged on the substrate, a through hole for the pressing plate mechanism to reciprocate in a linear motion along a set direction is further formed in the substrate, and a guide column is arranged between the passive pressing plate and the substrate. The guide column is arranged between the base plate and the passive plate to guide the movement of the passive plate, so that the linear movement of the passive plate is ensured. Therefore, the whole pressing plate mechanism can linearly move, the pushing acting force of the driven pressing plate on the kit is guaranteed not to deviate, and the puncture structure is guaranteed to puncture the sealing mechanism in the middle.
Preferably, the driving mechanism comprises a driving motor and a cam transmission assembly, the cam transmission assembly comprises an eccentric cam and a transmission piece, and the eccentric cam is arranged on an output shaft of the driving motor and driven to rotate around a set axis; one end of the transmission piece is arranged on the actuation pressing plate, the other end of the transmission piece is arranged on the eccentric cam, and the transmission piece drives the actuation pressing plate to perform reciprocating linear motion along the set direction under the driving of the eccentric cam. The pressing plate mechanism is driven by the driving motor and the cam transmission assembly, the rotation motion of the output shaft of the driving motor is converted into linear reciprocating motion of the pressing plate mechanism along the set direction by the cam transmission assembly, and therefore, the driving mechanism does not need a large lifting movement space and occupies a small area. In addition, because the eccentric cam rotates around the set axis, the motion is stable, and therefore, under the driving of the eccentric cam, the transmission piece drives the pressing plate mechanism to perform reciprocating linear motion, the stroke can be kept stable, and the error is reduced.
The invention also adopts the following technical scheme: the detection device for the kit comprises a shell, a support, an extraction and amplification assembly, a photoelectric assembly and the self-adaptive jacking assembly for the kit, wherein the support is arranged in the shell, and the extraction and amplification assembly, the photoelectric assembly and the jacking assembly are all arranged on the support. Due to the adoption of the self-adaptive jacking component, the detection device can ensure that a puncture structure in the kit is stable and powerful, a sealing structure is punctured and moves in place through jacking action, good conductivity is ensured after puncture, and the operation of the whipping reagent is smooth.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Drawings
FIG. 1 is a schematic structural diagram of an adaptive jacking component for a kit according to an embodiment;
FIG. 2 is an exploded view of the roof module of the embodiment;
FIG. 3 is a schematic view of the structure of the plate pressing mechanism in the embodiment;
FIG. 4 is a schematic diagram of a cartridge being pushed by the pushing assembly in the embodiment;
FIG. 5 is a schematic diagram of the set up of a reciprocating travel for the jacking assembly;
FIG. 6 is a cross-sectional view of the platen mechanism of FIG. 3;
FIG. 7 is a schematic diagram of a cam gear assembly according to an embodiment;
FIG. 8 is an external schematic view of the detection device of the pressing assembly provided by the application embodiment;
fig. 9 is an internal schematic view of the detection device of fig. 8.
Wherein: 1. drive mechanism, 10, drive motor, 11, eccentric cam, 110, first cam, 111, second cam, 112, drive slot, 12, drive member, 120, mounting shaft, 121, bearing, 2, actuation platen, 20, aperture, 21, guide ring, 210, blocking structure, 211, annular flange, 22, mounting hole, 3, passive platen, 4, resilient adjustment member, 40, connector, 400, limit boss, 41, resilient member, 5, base plate, 50, through hole, 51, guide post, 6, kit, 60, upper housing, 61, lower housing, 7, detection device, 70, housing, 71, bracket, 72, extraction amplification assembly, 73, optoelectronic assembly.
Detailed Description
Embodiments of the present invention are described in detail below, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar elements or elements having like or similar functions throughout. The embodiments described below by referring to the drawings are illustrative and intended to explain the present invention and should not be construed as limiting the invention.
Embodiments of the present invention are described below with reference to the accompanying drawings.
Examples: the present embodiment provides an adaptive pressing assembly for a kit, as shown in fig. 1 to 3, which includes a driving mechanism 1 and a pressing plate mechanism, wherein the pressing plate mechanism includes an actuation pressing plate 2, a passive pressing plate 3, and an elastic regulating member 4, and the actuation pressing plate 2 is driven by the driving mechanism 1 to reciprocate linearly in a set direction. The setting direction is determined according to the relative position of the pressing plate mechanism and the kit, and in this embodiment, the setting direction is a vertical direction, that is, the pressing plate mechanism performs reciprocating linear motion along the vertical direction, and the kit is placed under the pressing plate mechanism when in use. In other embodiments, the setting direction may be a horizontal direction, and the platen mechanism may reciprocate in the horizontal direction, so that the kit and the platen mechanism are placed horizontally when in use. The passive platen 3 moves linearly in a reciprocating manner along with the actuation platen 2, which means that the actuation platen 2 drives the passive platen 3 to move together when performing the reciprocating linear motion, so that the passive platen 3 presses the reagent kit 6. The actuating platen 2 can drive the driven platen 3 to reciprocate linearly, and the connection is achieved through an elastic adjusting member 4 arranged between the actuating platen 2 and the driven platen, and specifically, the elastic adjusting member 4 includes a connecting piece 40 and an elastic piece 41. Wherein the actuation pressure plate 2 and the connecting piece 40 are arranged in a relatively movable manner. The elastic member 41 always applies a force to the actuation platen 2 and the passive platen 3 in such a manner that the actuation platen 2 and the passive platen 3 have a tendency to move away from each other, that is, the elastic member 41 is always in a compressed state in which both ends of the elastic member 41 always apply a pushing force to the actuation platen 2 and the passive platen 3, respectively. And the link 40 is provided with a first stopper for restricting the detachment of the actuation presser plate 2 from the link 40. When the size of the reagent kit is larger due to tolerance, the distance between the actuating pressing plate 2 and the driven pressing plate 3 is changed by compressing the elastic piece 41 by the actuating pressing plate 2 in the pressing process, and the larger size of the reagent kit is counteracted by changing the distance between the actuating pressing plate 2 and the driven pressing plate, so that the self-adaption of reagent kits with different sizes is realized. The jacking of the kit is guaranteed to be in place, and meanwhile excessive jacking is avoided.
As shown in fig. 4 and 5, the stroke setting of the platen mechanism in the pressing assembly is described as follows: the jacking component is used for jacking the reagent kit to realize the conduction between the test tube inside the reagent kit and the extraction tank, the structure of the reagent kit is shown in fig. 4, the reagent kit 6 comprises an upper shell 60 and a lower shell 61, a puncture structure is arranged in the upper shell 60, a reagent tube is arranged in the lower shell 61, and a sealing film is arranged at the mouth of the reagent tube. The above-described structure of such a kit belongs to the prior art and is not described in detail here. Referring to fig. 5, the upper case 60 is required to be moved downward by a distance L with respect to the lower case 61 to pierce the sealing film by the piercing structure and allow the piercing structure to be inserted into a proper position in the reagent vessel (this position ensures smooth operation of the whipping reagent). Two different positions of the platen mechanism are shown in fig. 5, respectively, with the platen mechanism in the highest point of reciprocation and the platen mechanism moving downward to the point of immediate contact with the cartridge. The reciprocating linear motion stroke of the actuating platen 2 comprises a vertical downward stroke and a vertical upward stroke, the stroke amount of the actuating platen 2 in one stroke or in one stroke is set to be H, and the stroke H of the actuating platen 2 needs to be preset according to the pressing distance of the reagent kit 6 during design and manufacture. When the passive plate 3 is located at the highest position, the interval between the lower surface of the passive plate 3 and the upper surface of the upper case 60 is set to be P. Then ideally, H is designed to be h=l+p. However, in consideration of the factors such as the manufacturing tolerance, the expansion caused by the temperature change during the detection, and the like, H is designed as h=l+p+Δh in the present embodiment, where Δh is an unavoidable tolerance of the kit 6 during the manufacturing process, and may be obtained by measuring the batch-produced kit 6.
That is, when H is preset, the size of the smaller kit is set according to the tolerance, so that the smaller kit can be pressed in place. For those kits that are manufactured to a size greater than the design size, or that increase in size during the heating step, the above-described dimensional changes can be counteracted by compression of the elastic member. Therefore, the jacking component provided by the embodiment can jack the kit in place and cannot excessively jack the kit. In contrast, in the related art, the pressing plate mechanism that does not have the self-adaptive capability is generally designed to be h=l+p, and only a better pressing effect can be obtained for those kits that meet the design size requirement. The kit with smaller size due to tolerance can not be propped in place during manufacturing, the kit with larger size due to tolerance can be excessively propped during manufacturing, and correspondingly, the kit with increased size due to heating during detection can also be excessively propped.
As described above, the setting direction in the present embodiment refers to the vertical direction, so when the pushing assembly is applied, the reagent cartridge 6 is placed under the pressing plate mechanism, and the pressing plate mechanism moves downward to contact with the reagent cartridge 6 and push the reagent cartridge 6. The puncture structure inside the kit 6 punctures the sealing structure to conduct the reagent tube with the extraction tank. And then the stability of the kit is ensured through the jacking action of the jacking component on the kit until the pressing plate mechanism moves upwards to be separated from the kit 6 after the steps of mixing, amplifying and detecting are completed. And then the jacking operation is carried out after the next kit 6 is placed.
As described above, the elastic member in the pressing assembly provided in this embodiment is always in a compressed state, and in this embodiment, the pre-compression degree of the elastic member is specifically designed: the minimum pressing force required by the kit is set as Pmin, the maximum pressing force that the kit can bear is set as Pmax, and the force applied by the elastic member 41 to the passive plate 3 is set as F (the force F can represent the degree to which the elastic member 41 is compressed). When the passive plate 3 is not in contact with the kit, F has a minimum value Fmin; when the passive platen 3 presses against the cartridge and the actuation platen reaches the end of the stroke of the reciprocating linear motion (i.e. at the lowest point), F has a maximum value Fmax, where Pmin < Fmin < Fmax < Pmax.
In the whole puncturing process of puncturing the sealing film and extending into the proper position in the reagent tube, the puncturing structure needs to ensure that the puncturing is rapid and powerful, but can not be interrupted and blocked, and if the conditions of interruption and blocking occur, the possibility of leakage of the test solution can occur between the puncturing structure and the sealing film at the moment of interruption and blocking (the sealing can not be realized by rapid puncturing). The acting force F applied by the elastic member 41 to the passive plate 3 is the acting force applied by the passive plate 3 to the reagent kit 6, in this embodiment, fmin is set to be greater than Pmin, so that the passive plate 3 can be ensured to push the reagent kit 6 in a single direction all the time from the contact with the reagent kit 6 until the complete push is in place, and the elastic force of the elastic member 41 cannot be overcome to spring in the return direction due to the reverse acting force of the reagent kit 6. Therefore, the rapid, powerful and direct in-place jacking process of the passive plate 3 on the reagent kit 6 can be ensured, and the occurrence of leakage is avoided. It will be understood, of course, that after the jacking in place, if the kit expands due to heating during the detection, the expanded kit can apply a larger force to the passive platen, and the elastic member is compressed at this time to move the passive platen upward, so that the jacking assembly adapts to the dimensional change.
As shown in fig. 6, the actuation pressing plate 2 in the present embodiment is provided with a through hole 20 along a set direction, the connecting piece 40 passes through the through hole 20, the first limiting piece is a limiting boss 400 formed on one end of the connecting piece 40, a blocking structure 210 adapted to the limiting boss 400 is disposed in the through hole 20, and the blocking structure 210 is pressed against the limiting boss 400 under the action of the elastic piece 41. The connecting piece 40 is arranged through the through hole 20, so that the actuating pressing plate 2 can move relative to the connecting piece 40 against the elastic force of the elastic piece 41 under the drive of the driving mechanism 1; by providing the limit boss 400, the separation of the actuation platen 2 from the connector 40 can be restricted by the cooperation of the limit boss 400 and the blocking structure 210. In this embodiment, the limiting boss 400 is integrally formed with the connecting member 40, and it is understood that in other embodiments, the first limiting member may be fixedly mounted to the connecting member 40 by a fixed connection, for example, by screwing the first limiting member onto the connecting member 40, or by tightly fitting the first limiting member onto the connecting member 40. The shape of the first limiting member is not limited to the cylindrical shape shown in the present embodiment, but may be rectangular or other shapes, and can cooperate with the blocking structure 210 to perform a limiting function.
In addition, the other end of the connecting member 40 is fixedly disposed on the passive plate 3 in the present embodiment, so as to achieve the relative fixation of the connecting member 40 and the passive plate 3. Specifically, a stepped hole is formed in the passive plate 3, a bolt is inserted into the stepped hole, a threaded hole adapted to the bolt is formed in the end of the connecting member 40, and the passive plate 3 and the connecting member 40 are fixedly mounted through threaded connection. It will be appreciated that the connecting member 40 and the passive plate 3 may be fixedly connected by integral molding, tight fitting, or the like. Of course, one end of the connecting member 40 is provided with the limit boss 400, and the other end of the connecting member 40 is provided on the passive platen 3, so that at least one end of the connecting means of the two ends must be detachably connected to facilitate assembly of the actuation platen 2. In other embodiments, the following structure may be used to achieve the relative fixing arrangement of the connecting member and the passive plate: the connecting piece and the driven pressing plate are arranged in a relatively movable mode along the set direction, a second limiting piece used for limiting the driven pressing plate to be separated from the connecting piece is arranged at the other end of the connecting piece, and the driven pressing plate is pressed against the second limiting piece under the pressing of the elastic piece. The passive pressing plate can not move relative to the connecting piece under the action of the elastic piece on the passive pressing plate.
In this embodiment, a guide ring 21 adapted to the connecting member 40 is slidably sleeved outside the connecting member 40, the guide ring 21 is fixedly disposed on the actuation platen 2, specifically, an annular flange 211 is formed on the guide ring 21 in a radial extending manner, and the annular flange 211 is fixedly connected with the actuation platen 2 through bolts. The guide ring 21 has a blocking structure 210 which protrudes into the through hole 20, so that the actuating platen 2 is arranged outside the connecting piece 40 in a sliding manner by the guide ring 21, i.e. the movement direction of the actuating platen 2 is limited by the guide ring 21, so that a linear movement is ensured. The whole pressing plate mechanism moves linearly, so that the pushing acting force of the driven pressing plate 3 on the reagent kit is guaranteed not to deviate, and the puncture structure is guaranteed to puncture the sealing mechanism in the middle. Further, in the present embodiment, the elastic member 41 is a spring, which is sleeved outside the guide ring 21, and one end of the spring is provided on the annular flange 211 and the other end of the spring is provided on the passive plate 3. Therefore, the spring positioning device has a good positioning effect on the spring, and can prevent the spring from radial deflection in the compressed process, so that the direction of the acting force of the spring on the passive plate 3 is kept unchanged. In other embodiments, the elastic member 41 may be provided as an elastic member 41 such as a spring plate, and the elastic member 41 may be provided between the actuation platen 2 and the passive platen 3.
The guide ring 21 in this embodiment is a linear bearing, which can perform a good guiding function, and can reduce friction with the connecting member 40, thereby prolonging the service life. The linear bearing is directly manufactured into the annular flange 211 during manufacturing, and the size of the linear bearing is matched with the size of the through hole 20, that is, the upper section of the linear bearing can extend into the through hole 20, and the upper section of the linear bearing extending into the through hole 20 is the blocking structure 210.
The jacking assembly further comprises a base plate 5, the driving mechanism 1 is arranged on the base plate 5, a through hole 50 for the pressing plate mechanism to reciprocate in a linear motion along a set direction is further formed in the base plate 5, and a guide column 51 is arranged between the passive pressing plate 3 and the base plate 5. By providing the guide posts 51 between the substrate 5 and the passive plate 3, the movement of the passive plate 3 is guided, ensuring a linear movement thereof. Therefore, the whole pressing plate mechanism can linearly move, the pushing acting force of the driven pressing plate 3 on the reagent kit 6 is guaranteed not to deviate, and the puncture structure is guaranteed to puncture the sealing mechanism in the middle.
In this embodiment, the end face of the guide ring 21 facing the passive plate 3 and the passive plate 3 have an adaptive distance therebetween, and in order to make the entire pressing assembly advantageous in miniaturization and portability, the adaptive distance is set to 2mm in this embodiment. The inventor finds through experimental study that the dimensional change caused by tolerance, thermal expansion and the like generally does not exceed 0.5mm, so that the self-adaptive distance can be set to a selected value between 1mm and 5mm, the self-adaptive adjustment according to the size of the kit is fully ensured, and meanwhile, the whole mention of the top pressure assembly can be effectively reduced by setting smaller self-adaptive distance, so that the requirement of miniaturization of products is met.
As shown in fig. 1 and 7 in combination, the driving mechanism 1 in the present embodiment includes a driving motor 10 and a cam transmission assembly including an eccentric cam 11 and a transmission member 12, the eccentric cam 11 being provided on an output shaft of the driving motor 10 and driven to rotate about a set axis; one end of a transmission member 12 is arranged on the actuation pressing plate 2, the other end of the transmission member 12 is arranged on the eccentric cam 11, and the transmission member 12 drives the actuation pressing plate 2 to perform reciprocating linear motion along a set direction under the driving of the eccentric cam 11. The pressing plate mechanism is driven by the driving motor 10 and the cam transmission assembly, the rotation motion of the output shaft of the driving motor 10 is converted into linear reciprocating motion of the pressing plate mechanism along the set direction by the cam transmission assembly, and therefore the driving mechanism 1 does not need larger lifting and moving space and occupies smaller area. In addition, since the eccentric cam 11 rotates around the set axis, the motion is stable, so that the stroke of the driving member 12 driving the platen mechanism to perform the reciprocating linear motion can be kept stable under the driving of the eccentric cam, and the error is reduced.
In particular, in the present embodiment, the eccentric cam 11 includes a first cam 110 and a second cam 111, the first cam 110 is provided with a groove, the second cam 111 is formed in the groove, and a driving groove 112 is formed by the cooperation of the first cam 110 and the second cam 111. The transmission member 12 comprises a mounting shaft 120 and a bearing 121, wherein one end of the mounting shaft 120 is fixedly inserted on the actuating pressing plate 2, and specifically, a mounting hole 22 (combined with the one shown in fig. 3) matched with the mounting shaft 120 is arranged on the actuating pressing plate 2; the other end of the mounting shaft 120 is interference fit with the inner ring of the bearing 121, and the outer ring of the bearing 121 is rolling fit with the inner wall of the driving groove 112. Thus, when the driving motor 10 rotates, the eccentric cam 11 performs eccentric rotation motion around the axis of the output shaft of the driving motor 10, during the process, the inner wall of the driving groove 112 is in rolling contact with the outer ring of the bearing 121, and performs a pressing action on the bearing 121, and the bearing 121 drives the actuating pressing plate 2 to perform reciprocating linear motion. The advantage of such a drive structure is that, since the outer race of the bearing 121 is in rolling engagement with the inner wall of the drive groove 112, the wear of both is very small, thereby avoiding variations in the reciprocating linear motion stroke of the actuation platen 2 due to wear. Thus, the pressing plate mechanism can be further ensured to press the reagent kit 6 in place and can not excessively press the reagent kit.
In the case of detecting a sample in a reagent kit, the pressing assembly provided in this embodiment acts as a unit in the detection device to press the reagent kit, and schematic structural diagrams of the detection device using the pressing assembly are shown in fig. 8 and 9. The detection device 7 comprises a shell 70, a support 71, an extraction and amplification assembly 72, a photoelectric assembly 73 and the jacking assembly, wherein the support 71 is arranged in the shell 70, and the extraction and amplification assembly 72, the photoelectric assembly 73 and the jacking assembly are all arranged on the support 71. Due to the adoption of the self-adaptive jacking component, the detection device 7 can ensure that the puncture structure in the kit 6 is stable and powerful, the puncture sealing structure is penetrated and moved in place through the jacking action, and good conductivity is ensured after puncture, so that the operation of the whipping reagent is smooth.
When the detection is carried out, the jacking component is firstly translated through the sliding mechanism to avoid the position, then the kit is placed on the set station, and then the jacking component is translated to the original position, so that the pressing plate mechanism is positioned above the kit, and then the driving motor is controlled to drive the pressing plate mechanism to jack the kit. After the puncture is finished, the reagent tube is communicated with the extraction tank, and then the steps of mixing, amplifying, detecting and the like are carried out, wherein in the process, the steps of mixing and amplifying are finished by extracting the amplifying component, and the step of detecting is finished by the photoelectric component.
In the present invention, unless explicitly stated or limited otherwise in the examples, the terms "mounted," "connected," and "fixed" as used in the examples should be interpreted broadly, e.g., the connection may be a fixed connection, may be a removable connection, or may be integral, and it may be understood that the connection may also be a mechanical connection, an electrical connection, etc.; of course, it may be directly connected, or indirectly connected through an intermediate medium, or may be in communication with each other, or in interaction with each other. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art according to specific embodiments.
While embodiments of the present invention have been shown and described above, it will be understood that the above embodiments are illustrative and not to be construed as limiting the invention, and that variations, modifications, alternatives and variations may be made to the above embodiments by one of ordinary skill in the art within the scope of the invention.
Claims (11)
1. The utility model provides a self-adaptation roof pressure subassembly for kit, includes actuating mechanism (1) and clamp plate mechanism, its characterized in that, clamp plate mechanism includes:
An actuation platen (2) driven by the drive mechanism (1) to reciprocate in a linear direction;
a passive platen (3) which follows the actuation platen (2) for reciprocating linear movement and for pressing the cartridge; and
An elastic adjustment member (4) provided between the actuation platen (2) and the driven platen (3) and capable of adjusting a distance therebetween, the elastic adjustment member (4) including a connector (40) and an elastic piece (41);
The actuating pressing plate (2) and the connecting piece (40) are arranged in a relatively movable mode, the elastic piece (41) is always in a compressed state, two ends of the elastic piece are respectively applied to the actuating pressing plate (2) and the driven pressing plate (3), and the connecting piece (40) is provided with a first limiting piece used for limiting the actuating pressing plate (2) to be separated from the connecting piece (40);
The minimum jacking acting force required by the kit is Pmin, and the maximum jacking acting force bearable by the kit is Pmax;
The acting force applied by the elastic piece (41) to the passive plate (3) is F, the F has a minimum value Fmin when the passive plate (3) is not contacted with a reagent box, and the F has a maximum value Fmax when the passive plate (3) presses the reagent box and the actuating pressing plate (2) reaches the stroke end of the reciprocating linear motion, wherein Pmin < Fmin < Fmax < Pmax.
2. The self-adaptive jacking assembly for the kit according to claim 1, wherein the actuation pressing plate (2) is provided with a through hole (20) along a set direction, the connecting piece (40) passes through the through hole (20), the first limiting piece is a limiting boss (400) formed on one end of the connecting piece (40), a blocking structure (210) matched with the limiting boss (400) is arranged in the through hole (20), and the blocking structure (210) is abutted against the limiting boss (400) under the action of the elastic piece (41).
3. The self-adaptive jacking component for the kit according to claim 2, wherein the other end of the connecting piece (40) is fixedly arranged on the passive pressing plate (3); or alternatively, the first and second heat exchangers may be,
The connecting piece (40) and the driven pressing plate (3) are arranged in a relatively movable mode along a set direction, a second limiting piece used for limiting the driven pressing plate (3) to be separated from the connecting piece (40) is arranged at the other end of the connecting piece (40), and the driven pressing plate (3) is pressed against the second limiting piece under the pressing of the elastic piece (41).
4. The self-adaptive jacking assembly for a kit according to claim 2, characterized in that the connecting piece (40) is externally and slidably sleeved with a guide ring (21) matched with the connecting piece (40), the guide ring (21) is fixedly arranged on the actuating pressing plate (2), and the guide ring (21) is provided with a blocking structure (210) extending into the through hole (20).
5. An adaptive jacking assembly for a kit according to claim 4, characterized in that the end face of the guide ring (21) facing the passive platen (3) and the passive platen (3) have an adaptive pitch, which is a selected value between 1mm and 5mm.
6. The self-adaptive jacking assembly for the kit according to claim 4, wherein the guide ring (21) is formed with an annular flange (211) in a radial extending manner, and the annular flange (211) is fixedly connected with the actuating pressing plate (2) through bolts.
7. The self-adaptive jacking assembly for a kit according to claim 6, wherein the elastic member (41) is a spring, the spring is sleeved outside the guide ring (21), one end of the spring is arranged on the annular flange (211), and the other end of the spring is arranged on the passive plate (3).
8. The adaptive jacking assembly for kits as claimed in claim 4, wherein said guide ring (21) is a linear bearing.
9. The self-adaptive jacking assembly for the kit according to claim 1, further comprising a base plate (5), wherein the driving mechanism (1) is arranged on the base plate (5), a through hole (50) for the pressing plate mechanism to reciprocate linearly along a set direction is further formed in the base plate (5), and a guide column (51) is arranged between the passive pressing plate (3) and the base plate (5).
10. The self-adaptive jacking assembly for the kit according to claim 1, characterized in that the driving mechanism (1) comprises a driving motor (10) and a cam transmission assembly, the cam transmission assembly comprises an eccentric cam (11) and a transmission member (12), and the eccentric cam (11) is arranged on an output shaft of the driving motor (10) and driven to rotate around a set axis;
one end of the transmission piece (12) is arranged on the actuating pressing plate (2), the other end of the transmission piece (12) is arranged on the eccentric cam (11), and the transmission piece (12) drives the actuating pressing plate (2) to reciprocate in a linear motion along a set direction under the driving of the eccentric cam (11).
11. A detection device for a kit, comprising a housing (70), a bracket (71), an extraction and amplification assembly (72), a photoelectric assembly (73) and an adaptive jacking assembly for a kit according to any one of claims 1 to 10, wherein the bracket (71) is arranged in the housing (70), and the extraction and amplification assembly (72), the photoelectric assembly (73) and the jacking assembly are all arranged on the bracket (71).
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| CN202211145853.5A CN115651819B (en) | 2022-09-20 | 2022-09-20 | Self-adaptive jacking component for kit and detection device |
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| CN202211145853.5A CN115651819B (en) | 2022-09-20 | 2022-09-20 | Self-adaptive jacking component for kit and detection device |
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| CN115651819B true CN115651819B (en) | 2024-05-17 |
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