CN112795464A - Droplet generating device - Google Patents
Droplet generating device Download PDFInfo
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- CN112795464A CN112795464A CN202110126383.7A CN202110126383A CN112795464A CN 112795464 A CN112795464 A CN 112795464A CN 202110126383 A CN202110126383 A CN 202110126383A CN 112795464 A CN112795464 A CN 112795464A
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- chip
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- chip assembly
- pressing mechanism
- puncture needle
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- 230000007246 mechanism Effects 0.000 claims abstract description 101
- 238000000034 method Methods 0.000 claims abstract description 23
- 230000008569 process Effects 0.000 claims abstract description 20
- 239000012528 membrane Substances 0.000 claims abstract description 15
- 230000005540 biological transmission Effects 0.000 claims description 40
- 229920001296 polysiloxane Polymers 0.000 claims description 11
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 4
- 239000000741 silica gel Substances 0.000 claims description 4
- 229910002027 silica gel Inorganic materials 0.000 claims description 4
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 abstract description 3
- 229910052710 silicon Inorganic materials 0.000 abstract description 3
- 239000010703 silicon Substances 0.000 abstract description 3
- 229920006268 silicone film Polymers 0.000 abstract 1
- 230000009471 action Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000007847 digital PCR Methods 0.000 description 3
- BSFZSQRJGZHMMV-UHFFFAOYSA-N 1,2,3-trichloro-5-phenylbenzene Chemical compound ClC1=C(Cl)C(Cl)=CC(C=2C=CC=CC=2)=C1 BSFZSQRJGZHMMV-UHFFFAOYSA-N 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 230000003014 reinforcing effect Effects 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 239000013074 reference sample Substances 0.000 description 1
Images
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0241—Drop counters; Drop formers
- B01L3/0244—Drop counters; Drop formers using pins
Abstract
The invention provides a droplet generating device, which comprises a chip assembly, a pressing mechanism and a first driving mechanism; the first driving mechanism drives the pressing mechanism to move in the vertical direction; the pressing mechanism comprises a puncture needle, a chip pressing plate, a first elastic piece and a second elastic piece; when the chip assembly moves to the position right below the pressing mechanism, the pressing mechanism is pressed downwards, and air pressure is conducted through the puncture needle to the chip assembly so as to generate micro-droplets. In the process of pressing down the pressing mechanism, the first driving mechanism drives the pressing mechanism, and then the elastic force exerted by the first elastic piece drives the pressing mechanism to press the chip assembly, so that the functions of puncturing the silicone film and pressing the micro-droplet generation card are realized, and the micro-droplet generation card can be prevented from being crushed by rigid impact; in the process that the pressing mechanism leaves the chip assembly, the second elastic piece exerts reverse elasticity on the chip pressing plate, and in the process that the puncture needle leaves the silicon membrane, the chip pressing plate presses the chip assembly downwards to prevent the micro-droplet generation card from being brought up.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a droplet generation device.
Background
The digital PCR technology is a novel nucleic acid detection and quantification method, and the copy number of a target sequence is directly detected in an absolute quantification mode without depending on a standard curve and a reference sample. Principle of digital PCR: a standard PCR reaction is distributed into a large number of tiny reactors, each reactor contains or does not contain one or more copies of a target molecule (DNA template), so that single-molecule template PCR amplification is realized, and after the amplification is finished, the copy number of a target sequence is counted through the number of positive reactors. The digital PCR process comprises: droplet generation → overlay → amplification → droplet readout. Wherein droplet generation is used to generate thousands of droplets, using droplet generation apparatus. The prior art droplet-generating devices do not generate droplets at a fast rate, have unstable negative pressure, produce droplets with non-uniform consistency, and are prone to crushing of the droplet-generating card as it is being held down by a hold-down mechanism.
Disclosure of Invention
In view of the deficiencies of the prior art, it is an object of the present invention to provide a droplet generation apparatus.
The technical scheme of the invention is summarized as follows:
the invention provides a droplet generating device, which comprises a chip assembly, a pressing mechanism and a first driving mechanism; the first driving mechanism drives the pressing mechanism to move in the vertical direction;
the pressing mechanism comprises a puncture needle, a chip pressing plate, a first elastic piece and a second elastic piece;
when the chip assembly moves to the position right below the pressing mechanism, the pressing mechanism presses downwards, and air pressure is communicated through the puncture needle to introduce the air pressure into the chip assembly so as to generate micro-droplets;
in the process that the pressing mechanism presses the chip assembly, after the first driving mechanism drives the pressing mechanism to the first position, the pressing mechanism is driven to press the chip assembly by first elastic force exerted by the first elastic piece;
in the process that the pressing mechanism leaves the chip assembly, the first driving mechanism drives the pressing mechanism to move upwards, and the second elastic piece exerts second elastic force on the chip pressing plate, wherein the direction of the second elastic force is opposite to the moving direction of the puncture needle.
Furthermore, a through hole is formed in the chip pressing plate, and the puncture needle penetrates through the through hole and presses the chip assembly.
Furthermore, the interior of the puncture needle is of a hollow structure, and positive pressure or negative pressure can be introduced; the puncture needle is provided with a tip.
Furthermore, the pressing mechanism also comprises a transmission bracket and a pressing head; the puncture needle is positioned on the pressure head; the first driving mechanism drives the transmission bracket to move up and down;
the first elastic piece is positioned between the transmission bracket and the pressure head;
the second elastic piece is located between the transmission support and the chip pressing plate.
Further, the pressing head comprises a first pressing head and a second pressing head;
the bottom of the first pressure head is provided with a puncture needle, and a micro-channel is arranged inside the first pressure head and communicated with the puncture needle;
the first elastic piece is located between the transmission support and the second pressure head.
Further, the first pressure head is fixedly connected with the second pressure head.
Furthermore, a guide hole is formed in the second pressure head; the transmission support is provided with a guide shaft, the guide shaft is positioned in the guide hole, and the first elastic piece is positioned on the guide shaft.
Further, the transmission bracket comprises a first bracket and a second bracket, and the first bracket is positioned above the second bracket;
the first elastic piece is positioned between the first bracket and the pressure head;
the second elastic piece is located between the second support and the chip pressing plate.
Further, the chip component further comprises a second driving mechanism, and the second driving mechanism drives the chip component to move in the horizontal direction.
Further, the chip assembly comprises a micro-droplet generation card and a silicon film; the puncture needle punctures the silica gel membrane and introduces air pressure.
Compared with the prior art, the invention has the beneficial effects that: according to the droplet generation mechanism provided by the invention, the second driving mechanism drives the chip assembly to reach the pressing position, the first driving mechanism drives the pressing mechanism to move up and down, so that the puncture needle punctures the silica gel membrane, and the puncture needle is internally connected with ventilation pressure to introduce air pressure into the chip assembly to generate droplets; in the process of pressing down the pressing mechanism, the first driving mechanism drives the pressing mechanism, after the pressing mechanism contacts the chip assembly, the pressing mechanism is driven to press the chip assembly by the elastic force exerted by the first elastic piece, and the functions of puncturing the silicone membrane and pressing the micro-droplet generation card are realized, and meanwhile, the micro-droplet generation card can be prevented from being crushed by rigid impact; in the process that the pressing mechanism leaves the chip assembly, in the process that the first driving mechanism drives the pressing mechanism to move upwards, the second elastic piece exerts reverse elasticity on the chip pressing plate, and in the process that the puncture needle leaves the silicone membrane, the chip pressing plate presses the chip assembly downwards to prevent the micro-droplet generation card from being brought up.
The foregoing description is only an overview of the technical solutions of the present invention, and in order to make the technical solutions of the present invention more clearly understood and to implement them in accordance with the contents of the description, the following detailed description is given with reference to the preferred embodiments of the present invention and the accompanying drawings. The detailed description of the present invention is given in detail by the following examples and the accompanying drawings.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the invention without limiting the invention. In the drawings:
FIG. 1 is a schematic diagram of a droplet generator of the present invention;
FIG. 2 is another schematic diagram of a droplet generator of the present invention;
FIG. 3 is a schematic view of a portion of a droplet generator according to the present invention;
FIG. 4 is a schematic diagram of a chip assembly in a droplet generator of the invention.
Reference numerals: 10. a chip assembly; 11. a droplet generation card; 12. a silicone membrane; 13. a chip holder; 14. a slide rail; 15. a second drive motor; 17. a second transmission member;
21. a first drive motor; 22. a first transmission member; 23. pressing a chip plate; 231. a through hole; 24. puncturing needle; 25. a second elastic member; 26. a first ram; 27. a second ram; 271. a connecting portion; 2711. a guide hole; 272. a pressing part; 28. a transmission bracket; 281. a first bracket; 2811. a guide shaft; 282. a second bracket; 2821. a guide post; 29. a first elastic member;
31. a base plate; 32. a side bracket; 33. a vertical plate; 34. reinforcing ribs; 35. a gas tank; 36. an air pump; 37. a reflective light coupler; 38. and (7) a PCB board.
Detailed Description
The foregoing and other objects, features, aspects and advantages of the present invention will become more apparent from the following detailed description of the present invention when taken in conjunction with the accompanying drawings, which will enable those skilled in the art to practice the present invention with reference to the accompanying specification. In the drawings, the shape and size may be exaggerated for clarity, and the same reference numerals will be used throughout the drawings to designate the same or similar components. In the following description, terms such as center, thickness, height, length, front, back, rear, left, right, top, bottom, upper, lower, and the like are used based on the orientation or positional relationship shown in the drawings. In particular, "height" corresponds to the dimension from top to bottom, "width" corresponds to the dimension from left to right, and "depth" corresponds to the dimension from front to back. These relative terms are for convenience of description and are not generally intended to require a particular orientation. Terms concerning attachments, coupling and the like (e.g., "connected" and "attached") refer to a relationship wherein structures are secured or attached, either directly or indirectly, to one another through intervening structures, as well as both movable or rigid attachments or relationships, unless expressly described otherwise.
The present invention will be further described with reference to the accompanying drawings and the detailed description, and it should be noted that any combination of the embodiments or technical features described below can be used to form a new embodiment without conflict. It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
As shown in fig. 1-4, a droplet generation device of the present invention includes a chip assembly 10, a pressing mechanism, a first driving mechanism, and a second driving mechanism.
The droplet generation device further comprises a mounting frame on which the chip assembly 10, the hold-down mechanism, the first drive mechanism and the second drive mechanism are mounted. Wherein the first driving mechanism drives the pressing mechanism to move up and down in the longitudinal direction, and the second driving mechanism drives the chip assembly 10 to move in the horizontal direction.
The compressing mechanism comprises a puncture needle 24, a chip pressing plate 23, a first elastic member 29 and a second elastic member 24. Preferably, the chip pressure plate 23 is provided with a through hole 231, and the puncture needle 24 is pressed against the chip assembly 10 through the through hole 231. The interior of the puncture needle 24 is of a hollow structure, and positive pressure or negative pressure can be introduced; the puncture needle 24 is provided with a pointed end, and the silicone mold is easy to puncture.
The first driving mechanism drives the pressing mechanism to move up and down in the vertical direction; when the chip assembly 10 moves to the position right below the pressing mechanism, the pressing mechanism is pressed down, and air pressure is communicated through the puncture needle 24 to introduce the air pressure into the chip assembly for generating micro-droplets;
wherein, in the process that the pressing mechanism presses the chip component 10, after the first driving mechanism drives the pressing mechanism to the first position, the first elastic force exerted by the first elastic member 29 drives the pressing mechanism to press the chip component 10; wherein the first position is a position where the hold-down mechanism contacts the chip assembly; at this time, the first elastic force applied by the first elastic member 29 is the same as the direction in which the pressing mechanism presses the chip assembly 10.
During the process of separating the pressing mechanism from the chip assembly 10, the first driving mechanism drives the pressing mechanism to move upwards, and the second elastic member 25 exerts a second elastic force on the chip pressing plate, wherein the direction of the second elastic force is opposite to the moving direction of the puncture needle 24.
Specifically, the pressing mechanism further comprises a transmission bracket 28 and a pressing head. The puncture needle 24 is positioned on the pressure head; the first drive mechanism drives the transmission bracket 28 up and down to move the puncture needle 24 up and down.
If the puncture needle 24 is moved downward and the chip holder 23 is pressed downward against the chip module 10 by means of the driving frame 28 alone, the droplet-generating card 11 is easily broken by the rigid impact. Therefore, in the present invention, the first elastic member 29 and the second elastic member 25 are provided. The first elastic element 29 is positioned between the transmission bracket 28 and the pressure head; in the process that the transmission bracket 28 is driven to press downwards, the first elastic member 29 is in a compressed state, after the transmission bracket 28 is driven to press downwards to the position where the puncture needle 24 contacts the silicone membrane, the first elastic force released by the first elastic member 29 continuously drives the pressure head to press downwards for a certain distance, so that the pressure head presses the chip assembly 10, wherein the direction of the first elastic force is the same as the pressing direction of the transmission bracket 28.
The pressing head comprises a first pressing head 26 and a second pressing head 27; the first pressing head 26 is fixedly connected with the second pressing head 27, and the fixed connection mode can be a bolt connection mode. The bottom of the first pressure head 26 is provided with a puncture needle, the puncture needle 24 is positioned on the first pressure head 26, the bottom surface of the first pressure head 26 is provided with a small hole, the puncture needle 24 is sealed by AB glue to prevent air leakage, and the inside of the first pressure head 26 is provided with a micro-channel to connect the puncture needle 24. Preferably, the first pressure head 26 is provided with a first micropore for communicating positive pressure or negative pressure, and a second micropore for communicating atmosphere to realize balanced air pressure.
The first elastic element 29 is located between the transmission bracket 28 and the second ram. Specifically, the second ram 27 includes a connecting portion 271 and a pressing portion 272, the connecting portion 271 is fixedly connected to or integrally formed with the pressing portion 272, a guide hole 2711 is formed on the connecting portion 271 of the second ram 27, a guide shaft 2811 is mounted on the transmission bracket 28, the guide shaft 2811 is located in the guide hole 2711, and the first elastic element 29 is located on the guide shaft 2811. When the transmission bracket 28 is driven to be pressed down by the first driving mechanism, the second pressing head 27 sleeved on the guide shaft 2811 is pressed down along with the first pressing head 26 fixed with the second pressing head 27, after the pressing heads are pressed down to a certain distance, the first driving mechanism does not need to continuously drive the transmission bracket 28, at this time, after the first elastic force released by the first elastic piece 29 drives the first pressing head 26 and the second pressing head 27 to be pressed down for a certain distance, the puncture needle on the first pressing head 26 punctures the silicon film on the chip assembly 10 and realizes the function of compressing the chip assembly 10, and the micro-droplet generation card 11 on the chip assembly 10 is effectively prevented from being crushed by rigid impact.
The second elastic member 25 is located between the driving bracket 28 and the chip press plate 23. The chip holding plate 23 is mounted on the bottom surface of the first presser head 26 facing the chip module 10, a through hole is provided in the chip holding plate 23, and the puncture needle 24 is pressed against the chip module 10 through the through hole in the chip holding plate 23. A guide post 2821 is provided between the driving bracket 28 and the chip pressing plate 23, so that the chip pressing plate 23 can slide up and down relative to the driving bracket 28. The second elastic member 25 is sleeved on the guide post 2821, and when the transmission bracket 28 drives the first pressing head 26 to move upwards, the second elastic force released by the second elastic member 25 can enable the chip pressing plate 23 to continuously press the chip assembly 10.
Preferably, the first driving mechanism includes a first driving motor 21 and a first transmission member 22 connected to an output shaft of the first driving motor 21, the first transmission member 22 is of a common structure, and the transmission bracket 28 is mounted on the first transmission member 22, and under the action of the first driving motor 21, the bracket 28 moves up and down in a vertical direction in the drawing.
The driving bracket 28 includes a first bracket 281 and a second bracket 282, and the first bracket 281 is located above the second bracket 282.
The guide shaft 2811 is located between the first bracket 281 and the second bracket 282. The end of the first support 281 is mounted on the first transmission member 22, the guide shaft 2811 is mounted on the first support 281 in the direction toward the second support 282, the first elastic member 29 is located between the first support 281 and the pressing head, and the first elastic member 29 is sleeved on the guide shaft 2811, so that after the transmission support 28 is driven to a certain position, the first elastic force flexible driving pressing head released by the first elastic member 29 presses the chip assembly 10, and the crushing of the chip assembly 10 caused by the rigid impact of the transmission support 28 is avoided.
The second elastic member 25 is located between the second support 282 and the chip press plate 23. The side of the second support 282 is an L-shaped support, one end of the L-shaped support is a mounting part of the second support 282, the mounting part is fixed on the first transmission part 22, the other end of the L-shaped support is a guide post mounting part, a guide post 2821 is mounted on the guide post mounting part, a vacant press head moving region is arranged in the middle of the guide post mounting part, the press head moves up and down in the press head moving region, the second elastic part 25 is sleeved on the guide post 2821, one end of the second elastic part 25 is mounted on the second support 282, the other end of the second elastic part is fixed on the chip press plate 23, under the action of a second elastic force released by the second elastic part 25, the chip press plate 23 slides relative to the second support 282, when the first driving motor 21 drives the transmission support 28 to move upwards away from the chip assembly 10, the second elastic force released by the second elastic part 25 is opposite to the driving transmission support 28, the second elastic part continues to press the chip, carrying away the droplet generation card 11 on the chip assembly 10.
The chip assembly 10 comprises a droplet generation card 11, a silicone membrane 12 and a chip holder 13, wherein a puncture needle 24 is used for puncturing the silicone membrane 12 and introducing air pressure, and negative pressure is used for generating droplets in the droplet generation card by a PCR system and droplet generation oil in a reagent tube communicated with the droplet generation card. The droplet-generating card 11 and the silicone membrane 12 are fixed to the chip holder 13.
The mounting frame comprises a bottom plate 31, a side bracket 32, a vertical plate 33 and a reinforcing rib 34. The second driving mechanism comprises a second driving motor 15 and a second transmission member 17, the second transmission member 17 is provided with a sliding part, the bottom plate 31 is provided with a sliding rail 14, the second driving motor 15 drives the second transmission member 17 to slide along the sliding rail 14, and the chip holder 13 is installed on the second transmission member 17 so as to realize the movement of the chip holder 13 in the horizontal direction. The reflective light coupler 37 is disposed on the end surface or the bottom of the chip holder 13, and the reflective light coupler 37 is used for determining whether the chip holder 13 reaches a proper position.
The side support 32 is provided with an air tank 35, an air pump 36 and a PCB 38, the air tank 35 is used for storing negative pressure and providing negative pressure for the puncture needle 24, the air pump 36 is used for extracting the negative pressure of the air tank 35, and the PCB 38 is used for controlling a motor, a pump, an optical coupler and the like.
In addition, the number and type of the piercing needles 24 on the first ram 26 are matched to the chip assembly 10.
According to the droplet generation mechanism provided by the invention, the second driving mechanism drives the chip assembly 10 to reach the pressing position, the first driving mechanism drives the pressing mechanism to move up and down, so that the puncture needle 24 punctures the silica gel membrane 12, and the puncture needle 24 is internally connected with ventilation pressure to introduce air pressure into the chip assembly to generate droplets; in the process of pressing down the pressing mechanism, the first driving mechanism drives the pressing mechanism, after the pressing mechanism contacts the chip assembly 10, the pressing mechanism is driven to press the chip assembly 10 by the elasticity exerted by the first elastic piece 29, and the functions of puncturing the silicone membrane 12 and pressing the micro-droplet generation card are realized, and meanwhile, the micro-droplet generation card 11 can be prevented from being crushed by rigid impact; during the process of the pressing mechanism leaving the chip assembly 10, the second elastic member 25 exerts a reverse elastic force on the chip pressing plate 23 during the process of the first driving mechanism driving the pressing mechanism to move upward, and during the process of the puncture needle leaving the silicone membrane, the chip pressing plate 23 presses the chip assembly 10 downward, preventing the droplet generation card 11 from being carried up.
It should be noted that: the precedence order of the above embodiments of the present invention is only for description, and does not represent the merits of the embodiments. And specific embodiments thereof have been described above. Other embodiments are within the scope of the following claims. In some cases, the actions or steps recited in the claims may be performed in a different order than in the embodiments and still achieve desirable results. In addition, the processes depicted in the accompanying figures do not necessarily require the particular order shown, or sequential order, to achieve desirable results. In some embodiments, multitasking and parallel processing may also be possible or may be advantageous.
The embodiments in the present specification are described in a progressive manner, and the same and similar parts among the embodiments are referred to each other, and each embodiment focuses on the differences from the other embodiments. In particular, for the device and electronic apparatus embodiments, since they are substantially similar to the method embodiments, the description is relatively simple, and reference may be made to some descriptions of the method embodiments for relevant points.
The foregoing description has disclosed fully preferred embodiments of the present invention. It should be noted that those skilled in the art can make modifications to the embodiments of the present invention without departing from the scope of the appended claims. Accordingly, the scope of the appended claims is not to be limited to the specific embodiments described above.
Claims (10)
1. A droplet generation device comprising a chip assembly, a hold-down mechanism, a first drive mechanism; the first driving mechanism drives the pressing mechanism to move in the vertical direction;
the pressing mechanism comprises a puncture needle, a chip pressing plate, a first elastic piece and a second elastic piece;
when the chip assembly moves to the position right below the pressing mechanism, the pressing mechanism presses downwards, and air pressure is communicated through the puncture needle to introduce the air pressure into the chip assembly so as to generate micro-droplets;
in the process that the pressing mechanism presses the chip assembly, after the first driving mechanism drives the pressing mechanism to the first position, the pressing mechanism is driven to press the chip assembly by first elastic force exerted by the first elastic piece;
in the process that the pressing mechanism leaves the chip assembly, the first driving mechanism drives the pressing mechanism to move upwards, and the second elastic piece exerts second elastic force on the chip pressing plate, wherein the direction of the second elastic force is opposite to the moving direction of the puncture needle.
2. The droplet generating apparatus of claim 1 wherein said chip pressure plate has a through hole therethrough, said piercing needle being urged through said through hole toward said chip assembly.
3. A droplet generating apparatus according to claim 1, wherein the needle is hollow and is adapted to receive positive or negative pressure; the puncture needle is provided with a tip.
4. A droplet generator according to claim 1, wherein the hold-down mechanism further comprises a drive mount, a ram; the puncture needle is positioned on the pressure head; the first driving mechanism drives the transmission bracket to move up and down;
the first elastic piece is positioned between the transmission bracket and the pressure head;
the second elastic piece is located between the transmission support and the chip pressing plate.
5. A droplet generator according to claim 4, wherein the ram comprises a first ram and a second ram;
the bottom of the first pressure head is provided with a puncture needle, and a micro-channel is arranged inside the first pressure head and communicated with the puncture needle;
the first elastic piece is located between the transmission support and the second pressure head.
6. A droplet generator according to claim 5, wherein the first head is fixedly connected to the second head.
7. A droplet generator according to claim 5, wherein the second head includes guide holes; the transmission support is provided with a guide shaft, the guide shaft is positioned in the guide hole, and the first elastic piece is positioned on the guide shaft.
8. A droplet generator according to claim 4 wherein the actuator support comprises a first support and a second support, the first support being positioned above the second support;
the first elastic piece is positioned between the first bracket and the pressure head;
the second elastic piece is located between the second support and the chip pressing plate.
9. A droplet generation apparatus according to claim 1, further comprising a second drive mechanism for driving movement of the chip assembly in the horizontal direction.
10. The droplet generation apparatus of claim 1, wherein the chip assembly comprises a droplet generation card and a silicone membrane; the puncture needle punctures the silica gel membrane and introduces air pressure.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110126383.7A CN112795464B (en) | 2021-01-29 | 2021-01-29 | Droplet generation device |
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| CN202110126383.7A CN112795464B (en) | 2021-01-29 | 2021-01-29 | Droplet generation device |
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| CN112795464B CN112795464B (en) | 2024-02-02 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113368918A (en) * | 2021-06-21 | 2021-09-10 | 合肥瀚海星点生物科技有限公司 | Multi-channel liquid separation device and method based on microfluidic printing |
| CN115651819A (en) * | 2022-09-20 | 2023-01-31 | 杭州准芯生物技术有限公司 | Self-adaptive top pressure assembly for kit and detection device |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113368918A (en) * | 2021-06-21 | 2021-09-10 | 合肥瀚海星点生物科技有限公司 | Multi-channel liquid separation device and method based on microfluidic printing |
| CN113368918B (en) * | 2021-06-21 | 2022-04-26 | 合肥瀚海星点生物科技有限公司 | Multi-channel liquid separation device and method based on microfluidic printing |
| CN115651819A (en) * | 2022-09-20 | 2023-01-31 | 杭州准芯生物技术有限公司 | Self-adaptive top pressure assembly for kit and detection device |
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| CN112795464B (en) | 2024-02-02 |
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