CN112795464B - Droplet generation device - Google Patents
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- CN112795464B CN112795464B CN202110126383.7A CN202110126383A CN112795464B CN 112795464 B CN112795464 B CN 112795464B CN 202110126383 A CN202110126383 A CN 202110126383A CN 112795464 B CN112795464 B CN 112795464B
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- 230000007246 mechanism Effects 0.000 claims abstract description 101
- 238000000034 method Methods 0.000 claims abstract description 23
- 230000008569 process Effects 0.000 claims abstract description 20
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000000741 silica gel Substances 0.000 claims abstract description 13
- 229910002027 silica gel Inorganic materials 0.000 claims abstract description 13
- 230000005540 biological transmission Effects 0.000 claims description 42
- 239000012528 membrane Substances 0.000 claims description 5
- 229920001296 polysiloxane Polymers 0.000 claims description 3
- 239000011148 porous material Substances 0.000 claims description 2
- 108010025899 gelatin film Proteins 0.000 abstract description 11
- 230000006835 compression Effects 0.000 description 5
- 238000007906 compression Methods 0.000 description 5
- 238000009434 installation Methods 0.000 description 5
- 238000007847 digital PCR Methods 0.000 description 3
- 229920006268 silicone film Polymers 0.000 description 3
- BSFZSQRJGZHMMV-UHFFFAOYSA-N 1,2,3-trichloro-5-phenylbenzene Chemical compound ClC1=C(Cl)C(Cl)=CC(C=2C=CC=CC=2)=C1 BSFZSQRJGZHMMV-UHFFFAOYSA-N 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 230000003014 reinforcing effect Effects 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 239000013074 reference sample Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0241—Drop counters; Drop formers
- B01L3/0244—Drop counters; Drop formers using pins
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
Abstract
The invention provides a droplet generation device, which comprises a chip assembly, a pressing mechanism and a first driving mechanism; the first driving mechanism drives the pressing mechanism to move in the vertical direction; the pressing mechanism comprises a puncture needle, a chip pressing plate, a first elastic piece and a second elastic piece; when the chip assembly moves to the position right below the pressing mechanism, the pressing mechanism is pressed down, and the air pressure is conducted into the chip assembly through the puncture needle to conduct droplet generation. In the invention, in the pressing process of the pressing mechanism, the first driving mechanism drives the pressing mechanism, and the elastic force applied by the first elastic piece drives the pressing mechanism to press the chip assembly, so that the functions of puncturing the silica gel film and pressing the droplet generation card are realized, and meanwhile, the rigid impact and the droplet generation card crushing can be prevented; and in the process that the pressing mechanism leaves the chip assembly, the second elastic piece applies reverse elastic force to the chip pressing plate, and in the process that the puncture needle leaves the silica gel film, the chip pressing plate presses the chip assembly downwards to prevent the droplet generation card from being carried up.
Description
Technical Field
The invention relates to the field of biotechnology, in particular to a droplet generation device.
Background
The digital PCR technology is a new nucleic acid detection and quantification method, and adopts an absolute quantification mode to directly detect the copy number of a target sequence without depending on a standard curve and a reference sample. Principle of digital PCR: a standard PCR reaction is distributed into a large number of tiny reactors, one or a plurality of copies of target molecules (DNA templates) are contained or not contained in each reactor, so that single-molecule template PCR amplification is realized, and the copy number of target sequences is counted by the number of positive reactors after amplification is finished. The digital PCR process included: droplet generation- & gt membrane coating- & gt amplification- & gt droplet reading. Where droplet generation is used to generate thousands of droplets, is achieved using droplet generation devices. The droplet generation device in the prior art is not fast enough in droplet generation speed, unstable in negative pressure, uneven in generated droplet consistency and easy to crush a droplet generation card when the pressing mechanism presses the droplet generation card.
Disclosure of Invention
In view of the shortcomings of the prior art, it is an object of the present invention to provide a droplet generation device.
The technical scheme of the invention is summarized as follows:
the invention provides a droplet generation device, which comprises a chip assembly, a pressing mechanism and a first driving mechanism; the first driving mechanism drives the pressing mechanism to move in the vertical direction;
the pressing mechanism comprises a puncture needle, a chip pressing plate, a first elastic piece and a second elastic piece;
when the chip assembly moves to the position right below the pressing mechanism, the pressing mechanism is pressed down, and the puncture needle is connected with air pressure to guide the air pressure into the chip assembly so as to generate micro drops;
in the process that the pressing mechanism presses the chip assembly, after the first driving mechanism drives the pressing mechanism to a first position, the first elastic force applied by the first elastic piece drives the pressing mechanism to press the chip assembly;
in the process that the pressing mechanism leaves the chip assembly, the first driving mechanism drives the pressing mechanism to move upwards, and the second elastic piece applies second elastic force to the chip pressing plate, wherein the direction of the second elastic force is opposite to the movement direction of the puncture needle.
Further, a through hole is formed in the chip pressing plate, and the puncture needle penetrates through the through hole and presses the chip assembly.
Further, the puncture needle is of a hollow structure and can be introduced with positive pressure or negative pressure; the puncture needle is provided with a pointed head.
Further, the compressing mechanism also comprises a transmission bracket and a pressure head; the puncture needle is positioned on the pressure head; the first driving mechanism drives the transmission bracket to move up and down;
the first elastic piece is positioned between the transmission bracket and the pressure head;
the second elastic piece is positioned between the transmission bracket and the chip pressing plate.
Further, the pressure head comprises a first pressure head and a second pressure head;
the bottom of the first pressure head is provided with a puncture needle, and a micro-pore canal is arranged in the first pressure head and is communicated with the puncture needle;
the first elastic piece is located between the transmission support and the second pressure head.
Further, the first pressure head is fixedly connected with the second pressure head.
Further, a guide hole is formed in the second pressure head; the transmission support is provided with a guide shaft, the guide shaft is positioned in the guide hole, and the first elastic piece is positioned on the guide shaft.
Further, the transmission bracket comprises a first bracket and a second bracket, and the first bracket is positioned above the second bracket;
the first elastic piece is positioned between the first bracket and the pressure head;
the second elastic piece is positioned between the second bracket and the chip pressing plate.
Further, the device also comprises a second driving mechanism, wherein the second driving mechanism drives the chip assembly to move in the horizontal direction.
Further, the chip assembly includes a droplet generation card and a silicone film; the puncture needle punctures the silica gel membrane and introduces air pressure.
Compared with the prior art, the invention has the beneficial effects that: according to the droplet generation mechanism provided by the invention, the chip assembly is driven to reach the compression position by the second driving mechanism, the compression mechanism is driven to move up and down by the first driving mechanism, so that the puncture needle punctures the silica gel film, and the puncture needle is connected with air pressure to guide the air pressure into the chip assembly for droplet generation; in the pressing process of the pressing mechanism, the first driving mechanism drives the pressing mechanism, and after the pressing mechanism contacts the chip assembly, the elastic force applied by the first elastic piece drives the pressing mechanism to press the chip assembly, so that the functions of puncturing the silica gel film and pressing the droplet generation card are realized, and meanwhile, the rigid impact crushing of the droplet generation card can be prevented; in the process that the pressing mechanism leaves the chip assembly, in the process that the first driving mechanism drives the pressing mechanism to move upwards, the second elastic piece applies reverse elastic force to the chip pressing plate, and in the process that the puncture needle leaves the silica gel film, the chip pressing plate presses the chip assembly downwards to prevent the droplet generation card from being carried up.
The foregoing description is only an overview of the present invention, and is intended to provide a better understanding of the present invention, as it is embodied in the following description, with reference to the preferred embodiments of the present invention and the accompanying drawings. Specific embodiments of the present invention are given in detail by the following examples and the accompanying drawings.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiments of the invention and together with the description serve to explain the invention and do not constitute a limitation on the invention. In the drawings:
FIG. 1 is a schematic diagram of a droplet generation device according to the present invention;
fig. 2 is a schematic view of another construction of a droplet generation device of the invention;
FIG. 3 is a schematic view of a portion of the structure of a droplet generation device of the present invention;
fig. 4 is a schematic view of a chip assembly in a droplet generation device of the invention.
Reference numerals: 10. a chip assembly; 11. a droplet generation card; 12. a silicone film; 13. a chip holder; 14. a slide rail; 15. a second driving motor; 17. a second transmission member;
21. a first driving motor; 22. a first transmission member; 23. a chip pressing plate; 231. a through hole; 24. a puncture needle; 25. a second elastic member; 26. a first ram; 27. a second ram; 271. a connection part; 2711. a guide hole; 272. a pressing part; 28. a transmission bracket; 281. a first bracket; 2811. a guide shaft; 282. a second bracket; 2821. a guide post; 29. a first elastic member;
31. a bottom plate; 32. a side bracket; 33. a vertical plate; 34. reinforcing ribs; 35. a gas tank; 36. an air pump; 37. a reflective optocoupler; 38. and a PCB board.
Detailed Description
The foregoing and other objects, features, aspects and advantages of the present invention will become more apparent to those skilled in the art from the following detailed description, which, taken in conjunction with the annexed drawings, discloses a device for practicing the invention. In the drawings, the shape and size may be exaggerated for clarity, and the same reference numerals will be used throughout the drawings to designate the same or similar components. In the following description, terms such as center, thickness, height, length, front, back, rear, left, right, top, bottom, upper, lower, etc. are based on the orientation or positional relationship shown in the drawings. In particular, "height" corresponds to the top-to-bottom dimension, "width" corresponds to the left-to-right dimension, and "depth" corresponds to the front-to-back dimension. These relative terms are for convenience of description and are not generally intended to require a particular orientation. Terms (e.g., "connected" and "attached") referring to an attachment, coupling, etc., refer to a relationship wherein these structures are directly or indirectly secured or attached to one another through intervening structures, as well as both movable or rigid attachments or relationships, unless expressly described otherwise.
The present invention will be further described with reference to the accompanying drawings and detailed description, wherein it is to be understood that, on the premise of no conflict, new embodiments may be formed by any combination of the embodiments or technical features described below. It will be understood that terms, such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
As shown in fig. 1-4, a droplet generation device of the present invention includes a chip assembly 10, a hold-down mechanism, a first drive mechanism, and a second drive mechanism.
The droplet generation device further comprises a mounting frame on which the chip assembly 10, the pressing mechanism, the first driving mechanism and the second driving mechanism are all mounted. Wherein the first driving mechanism drives the pressing mechanism to move up and down in the longitudinal direction, and the second driving mechanism drives the chip assembly 10 to move in the horizontal direction.
The pressing mechanism comprises a puncture needle 24, a chip pressing plate 23, a first elastic piece 29 and a second elastic piece 24. Preferably, the chip pressing plate 23 is provided with a through hole 231, and the puncture needle 24 is pressed toward the chip assembly 10 through the through hole 231. The puncture needle 24 is hollow and can be introduced with positive pressure or negative pressure; the puncture needle 24 is provided with a pointed tip, which easily punctures the silicone mold.
The first driving mechanism drives the pressing mechanism to move up and down in the vertical direction; when the chip assembly 10 moves to the position right below the pressing mechanism, the pressing mechanism is pressed down, and the air pressure is conducted to the chip assembly through the puncture needle 24 to conduct droplet generation;
wherein, in the process that the pressing mechanism presses the chip assembly 10, the first driving mechanism drives the pressing mechanism to the first position, and then the first elastic force applied by the first elastic piece 29 drives the pressing mechanism to press the chip assembly 10; the first position is a position where the pressing mechanism contacts the chip assembly; at this time, the first elastic force applied by the first elastic member 29 is the same as the direction in which the pressing mechanism is pressed toward the chip assembly 10.
During the process that the pressing mechanism leaves the chip assembly 10, the first driving mechanism drives the pressing mechanism to move upwards, and the second elastic piece 25 applies a second elastic force to the chip pressing plate, wherein the direction of the second elastic force is opposite to the movement direction of the puncture needle 24.
Specifically, the compressing mechanism further comprises a transmission bracket 28 and a pressure head. The puncture needle 24 is positioned on the compression head; the first drive mechanism drives the drive carriage 28 up and down to move the lancet 24 up and down.
If the puncture needle 24 is driven to move downwards by the transmission bracket 28 and the chip pressing plate 23 presses the chip assembly 10 downwards, the droplet generation card 11 is easily broken due to rigid impact. Therefore, in the present invention, the first elastic member 29 and the second elastic member 25 are provided. The first elastic piece 29 is positioned between the transmission bracket 28 and the pressure head; in the process that the transmission support 28 is driven to press down, the first elastic piece 29 is in a compressed state, after the transmission support 28 is driven to press down to the position where the puncture needle 24 contacts the silica gel film, the first elastic force released by the first elastic piece 29 continuously drives the pressing head to press down for a certain distance, so that the pressing head presses down the chip assembly 10, wherein the direction of the first elastic force is the same as the pressing down direction of the transmission support 28.
The ram comprises a first ram 26 and a second ram 27; the first ram 26 is fixedly connected to the second ram 27, which may be a bolted connection. The bottom of first pressure head 26 installs the pjncture needle, and pjncture needle 24 is located first pressure head 26, and the bottom surface of first pressure head 26 is equipped with the aperture, seals pjncture needle 24 through AB glue, prevents gas leakage, and the inside of first pressure head 26 is equipped with the micropore, switch on pjncture needle 24. Preferably, the first pressure head 26 is provided with a first micro-hole for communicating positive or negative pressure and a second micro-hole for communicating with the atmosphere to achieve an equilibrium air pressure.
The first elastic member 29 is located between the transmission bracket 28 and the second ram. Specifically, the second pressure head 27 includes a connection portion 271 and a pressing portion 272, the connection portion 271 and the pressing portion 272 are fixedly connected or integrally formed, a guide hole 2711 is formed in the connection portion 271 of the second pressure head 27, a guide shaft 2811 is mounted on the transmission bracket 28, the guide shaft 2811 is located in the guide hole 2711, and the first elastic member 29 is located on the guide shaft 2811. When the transmission support 28 is driven to press down by the first driving mechanism, the second pressing head 27 sleeved on the guide shaft 2811 is pressed down along with the second pressing head 27, the first driving mechanism does not need to continuously drive the transmission support 28 after the pressing head is pressed down to a certain distance, and after the first elastic force released by the first elastic piece 29 drives the first pressing head 26 and the second pressing head 27 to press down to a certain distance, the puncture needle on the first pressing head 26 punctures the silica gel film on the chip assembly 10 and achieves the function of pressing the chip assembly 10, so that the rigid impact is effectively prevented from crushing the micro-droplet generating card 11 on the chip assembly 10.
The second elastic member 25 is located between the transmission bracket 28 and the chip pressing plate 23. The chip pressing plate 23 is mounted on the bottom surface of the first pressing head 26 facing the direction of the chip assembly 10, a through hole is formed in the chip pressing plate 23, and the puncture needle 24 penetrates through the through hole in the chip pressing plate 23 to press the chip assembly 10. A guide post 2821 is provided between the transmission bracket 28 and the chip pressing plate 23, so that the chip pressing plate 23 can slide up and down relative to the transmission bracket 28. The second elastic member 25 is sleeved on the guide post 2821, and when the transmission bracket 28 drives the first pressing head 26 to move upwards, the second elastic force released by the second elastic member 25 can enable the chip pressing plate 23 to continuously press the chip assembly 10.
Preferably, the first driving mechanism comprises a first driving motor 21 and a first transmission member 22 connected with an output shaft of the first driving motor 21, the first transmission member 22 is of a common structure, a transmission bracket 28 is mounted on the first transmission member 22, and the bracket 28 moves up and down in the vertical direction in the figure under the action of the first driving motor 21.
The transmission bracket 28 comprises a first bracket 281 and a second bracket 282, and the first bracket 281 is located above the second bracket 282.
The guide shaft 2811 is located between the first bracket 281 and the second bracket 282. The end of the first support 281 is mounted on the first transmission member 22, the first support 281 is provided with a guide shaft 2811 towards the direction of the second support 282, the first elastic member 29 is located between the first support 281 and the pressing head, the first elastic member 29 is sleeved on the guide shaft 2811, and after the transmission support 28 is driven to a certain position, the first elastic force released by the first elastic member 29 flexibly drives the pressing head to press the chip assembly 10, so that the chip assembly 10 is prevented from being crushed due to rigid impact of the transmission support 28.
The second elastic member 25 is located between the second bracket 282 and the chip pressing plate 23. The side of second support 282 is L shape support, the one end of L shape support is the installation department of second support 282, the installation department is fixed on first driving medium 22, the other end of L shape support is the guide pillar installation department, install guide pillar 2821 on the guide pillar installation department, the centre of guide pillar installation department is the pressure head movable region of vacancy, the pressure head is movable from top to bottom in the pressure head movable region, second elastic component 25 suit is on guide pillar 2821, the one end of second elastic component 25 is installed on second support 282, the other end is fixed on chip clamp plate 23, under the second elastic force that second elastic component 25 released, chip clamp plate 23 slides relatively relative to second support 282, when first driving motor 21 drives driving medium support 28 upwards and leaves chip assembly 10, the second elastic force that second elastic component 25 released is opposite with the direction of driving medium support 28, continue to push down chip assembly 10, prevent pjncture needle 24 from leaving on the chip assembly 10, take away the microdroplet on the chip assembly 10 and generate card 11.
The chip assembly 10 comprises a droplet generation card 11, a silica gel film 12 and a chip holder 13, wherein a puncture needle 24 is used for puncturing the silica gel film 12 and introducing air pressure, and negative pressure is used for generating droplets in the droplet generation card by a PCR system and droplet generation oil in a reagent tube communicated with the droplet generation card. The droplet generation card 11 and the silicone membrane 12 are fixed on the chip carrier 13.
The mounting frame comprises a bottom plate 31, side brackets 32, vertical plates 33 and reinforcing ribs 34. The second driving mechanism comprises a second driving motor 15 and a second transmission member 17, the second transmission member 17 is provided with a sliding member, a sliding rail 14 is arranged on the bottom plate 31, the second driving motor 15 drives the second transmission member 17 to slide along the sliding rail 14, and the chip carrier 13 is mounted on the second transmission member 17 so as to realize the movement of the chip carrier 13 in the horizontal direction. The end surface or the bottom of the chip holder 13 is provided with a reflective optical coupler 37, and the reflective optical coupler 37 is used for judging whether the chip holder 13 reaches a proper position.
The side bracket 32 is provided with a gas tank 35, an air pump 36 and a PCB 38, the gas tank 35 is used for storing negative pressure to provide negative pressure for the puncture needle 24, the air pump 36 is used for extracting the negative pressure of the gas tank 35, and the PCB 38 is used for controlling a motor, a pump, an optocoupler and the like.
In addition, the number and type of piercing pins 24 on the first ram 26 are matched to the chip assembly 10.
According to the droplet generation mechanism provided by the invention, the chip assembly 10 is driven to reach the compression position by the second driving mechanism, the compression mechanism is driven to move up and down by the first driving mechanism, so that the puncture needle 24 punctures the silica gel film 12, and the puncture needle 24 is connected with air pressure to guide the air pressure into the chip assembly for droplet generation; in the pressing down process of the pressing mechanism, the first driving mechanism drives the pressing mechanism, and after the pressing mechanism contacts the chip assembly 10, the elastic force applied by the first elastic piece 29 drives the pressing mechanism to press the chip assembly 10, so that the functions of puncturing the silica gel film 12 and pressing the droplet generation card are realized, and meanwhile, the rigid impact is prevented from crushing the droplet generation card 11; during the process that the pressing mechanism leaves the chip assembly 10, the second elastic member 25 applies reverse elastic force to the chip pressing plate 23 during the process that the first driving mechanism drives the pressing mechanism to move upwards, and during the process that the puncture needle leaves the silicone film, the chip pressing plate 23 presses down the chip assembly 10 to prevent the droplet generation card 11 from being carried up.
It should be noted that: the sequence of the embodiments of the present invention is only for description, and does not represent the advantages and disadvantages of the embodiments. And the foregoing description has been directed to specific embodiments of this specification. Other embodiments are within the scope of the following claims. In some cases, the actions or steps recited in the claims can be performed in a different order than in the embodiments and still achieve desirable results. In addition, the processes depicted in the accompanying figures do not necessarily require the particular order shown, or sequential order, to achieve desirable results. In some embodiments, multitasking and parallel processing are also possible or may be advantageous.
In this specification, each embodiment is described in a progressive manner, and identical and similar parts of each embodiment are all referred to each other, and each embodiment mainly describes differences from other embodiments. In particular, for the apparatus and electronic device embodiments, since they are substantially similar to the method embodiments, the description is relatively simple, and references to the parts of the description of the method embodiments are only required.
The foregoing description has fully disclosed specific embodiments of this invention. It should be noted that any modifications to the specific embodiments of the invention may be made by those skilled in the art without departing from the scope of the invention as defined in the appended claims. Accordingly, the scope of the claims of the present invention is not limited to the foregoing detailed description.
Claims (8)
1. A droplet generation device comprising a chip assembly, a hold-down mechanism, a first drive mechanism; the first driving mechanism drives the pressing mechanism to move in the vertical direction;
the pressing mechanism comprises a puncture needle, a chip pressing plate, a first elastic piece, a second elastic piece, a transmission bracket and a pressing head;
the puncture needle is positioned on the pressure head;
the chip pressing plate is provided with a through hole, and the puncture needle passes through the through hole and presses the chip assembly;
the first driving mechanism drives the transmission bracket to move up and down;
the first elastic piece is positioned between the transmission bracket and the pressure head;
the second elastic piece is positioned between the transmission bracket and the chip pressing plate;
when the chip assembly moves to the position right below the pressing mechanism, the pressing mechanism is pressed down, and the puncture needle is connected with air pressure to guide the air pressure into the chip assembly so as to generate micro drops;
in the process that the pressing mechanism presses the chip assembly, after the first driving mechanism drives the pressing mechanism to a first position, the first elastic force applied by the first elastic piece drives the pressing mechanism to press the chip assembly;
in the process that the pressing mechanism leaves the chip assembly, the first driving mechanism drives the pressing mechanism to move upwards, and the second elastic piece applies second elastic force to the chip pressing plate, wherein the direction of the second elastic force is opposite to the movement direction of the puncture needle.
2. A droplet generator according to claim 1, wherein the needle is hollow and is capable of introducing positive or negative pressure; the puncture needle is provided with a pointed head.
3. A droplet generation device according to claim 1, wherein the ram comprises a first ram and a second ram;
the bottom of the first pressure head is provided with a puncture needle, and a micro-pore canal is arranged in the first pressure head and is communicated with the puncture needle;
the first elastic piece is located between the transmission support and the second pressure head.
4. A droplet generator according to claim 3, wherein the first ram is fixedly connected to the second ram.
5. A droplet-generating device according to claim 3, wherein the second ram is provided with a pilot hole; the transmission support is provided with a guide shaft, the guide shaft is positioned in the guide hole, and the first elastic piece is positioned on the guide shaft.
6. A droplet generator according to claim 1, wherein the drive support comprises a first support and a second support, the first support being located above the second support;
the first elastic piece is positioned between the first bracket and the pressure head;
the second elastic piece is positioned between the second bracket and the chip pressing plate.
7. A droplet generation device according to claim 1, further comprising a second drive mechanism, the second drive mechanism driving movement of the chip assembly in a horizontal direction.
8. A droplet generator according to claim 1, wherein said chip assembly comprises a droplet generator card and a silicone membrane; the puncture needle punctures the silica gel membrane and introduces air pressure.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110126383.7A CN112795464B (en) | 2021-01-29 | 2021-01-29 | Droplet generation device |
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|---|---|---|---|
| CN202110126383.7A CN112795464B (en) | 2021-01-29 | 2021-01-29 | Droplet generation device |
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| CN112795464A CN112795464A (en) | 2021-05-14 |
| CN112795464B true CN112795464B (en) | 2024-02-02 |
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| CN113368918B (en) * | 2021-06-21 | 2022-04-26 | 合肥瀚海星点生物科技有限公司 | Multi-channel liquid separation device and method based on microfluidic printing |
| CN115651819B (en) * | 2022-09-20 | 2024-05-17 | 杭州准芯生物技术有限公司 | Self-adaptive jacking component for kit and detection device |
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| CN215140008U (en) * | 2021-01-29 | 2021-12-14 | 中国科学院苏州生物医学工程技术研究所 | Droplet generating device |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US7010391B2 (en) * | 2001-03-28 | 2006-03-07 | Handylab, Inc. | Methods and systems for control of microfluidic devices |
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|---|---|---|---|---|
| CN110760438A (en) * | 2019-11-05 | 2020-02-07 | 中国科学院苏州生物医学工程技术研究所 | Droplet generating device |
| CN212102841U (en) * | 2019-11-05 | 2020-12-08 | 苏州中科医疗器械产业发展有限公司 | Droplet generating device |
| CN215140008U (en) * | 2021-01-29 | 2021-12-14 | 中国科学院苏州生物医学工程技术研究所 | Droplet generating device |
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| CN112795464A (en) | 2021-05-14 |
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