CN212128182U - Micro-fluidic chip nucleic acid detection device - Google Patents

Abstract

The utility model provides a micro-fluidic chip nucleic acid detection device, relating to the technical field of nucleic acid detection, which comprises a top plate, a bottom plate and a movable middle plate; a guide piece is arranged between the top plate and the bottom plate, and the middle plate is arranged on the guide piece; the testing card is provided with a biochip, a PCR (polymerase chain reaction) chamber, a piston chamber, a cracking chamber and a plurality of accommodating chambers, the biochip, the cracking chamber and the accommodating chambers are communicated with the piston chamber through runners, pistons are arranged in the piston chambers, and the valve driving assembly is used for controlling the opening and closing of valves in each runner; the top plate is provided with a displacement assembly for driving the middle plate to move up and down, the top plate is also provided with a piston driving assembly capable of moving up and down, and the piston driving assembly is used for driving a piston to move; the middle plate is provided with a cracking component which can drive the cracking component in the cracking chamber to rotate. The technical effect of high working efficiency is achieved.

Description

Micro-fluidic chip nucleic acid detection device

Technical Field

The utility model relates to a nucleic acid detects technical field, particularly, relates to micro-fluidic chip nucleic acid detection device.

Background

The nucleic acid detection technology is a general term of a series of technologies for directly detecting pathogen nucleic acid, is a detection method with high sensitivity, high specificity and large detection flux, and is widely applied to virus detection, congenital genetic disease diagnosis, paternity test and the like in the current clinical medicine field.

Nucleic acid detection is generally divided into three steps, namely nucleic acid extraction, nucleic acid amplification and nucleic acid detection. At present, most of commercial nucleic acid detection reagent products are independently used for nucleic acid extraction, nucleic acid amplification and nucleic acid detection, a sample is moved to subsequent equipment for subsequent steps after the previous step is completed, in order to avoid pollution, the steps are generally required to be completed in different areas or even different laboratories, and strict requirements are required on the transfer sequence of the sample, so that the whole nucleic acid detection process cannot be smoothly carried out due to the operation mode, and the characteristics of complex operation, low efficiency and the like exist generally.

Therefore, it is an important technical problem to be solved by those skilled in the art to provide a microfluidic chip nucleic acid detection device with high working efficiency.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a micro-fluidic chip nucleic acid detecting device to alleviate the technical problem that work efficiency is low among the prior art.

In a first aspect, an embodiment of the present invention provides a microfluidic chip nucleic acid detecting apparatus, including a top plate, a bottom plate, and a movable middle plate;

a guide piece is arranged between the top plate and the bottom plate, one end of the guide piece is connected with the top plate, the other end of the guide piece is connected with the bottom plate, and the intermediate plate is arranged on the guide piece;

the testing card is provided with a biochip, a PCR (polymerase chain reaction) chamber, a piston chamber, a cracking chamber and a plurality of accommodating chambers, the biochip, the cracking chamber and the accommodating chambers are communicated with the piston chamber through runners, a piston is arranged in the piston chamber, and the valve driving assembly is used for controlling the opening and closing of valves in each runner;

the top plate is provided with a displacement assembly for driving the middle plate to move up and down, and the top plate is also provided with a piston driving assembly capable of moving up and down, and the piston driving assembly is used for driving a piston to move;

the middle plate is provided with a cracking component which can drive the cracking component in the cracking chamber to rotate.

With reference to the first aspect, embodiments of the present invention provide a possible implementation manner of the first aspect, wherein the displacement assembly includes a cam motor, a cam shaft, a cam, and a first reset member;

the first reset piece is sleeved on the guide piece, one end of the first reset piece is abutted against the bottom plate, and the other end of the first reset piece is abutted against the middle plate;

the lower surface of the top plate is provided with a camshaft mounting plate, the cam motor is in transmission connection with the camshaft, the cam is mounted on the camshaft, and the middle plate is provided with a cam follower matched with the cam;

the camshaft mounting panel is provided with a first micro switch, and the camshaft is provided with a first contact rod for triggering the first micro switch.

With reference to the first aspect, embodiments of the present invention provide a possible implementation manner of the first aspect, wherein the piston driving assembly includes a lead screw motor, a lead screw, and a piston driving member;

the screw motor is connected with the screw, and a screw nut matched with the screw is fixedly arranged on the piston driving piece.

In combination with the first aspect, an embodiment of the present invention provides a possible implementation manner of the first aspect, wherein the cracking assembly includes a cracking motor, a cracking output shaft, and a cracking mounting plate;

the cracking mounting plate is mounted on the intermediate plate through a support column, the cracking motor is mounted on the cracking mounting plate, and the cracking output shaft is in transmission connection with the cracking motor;

one end of the cracking output shaft penetrates through and protrudes out of the middle plate.

In combination with the first aspect, an embodiment of the present invention provides a possible implementation manner of the first aspect, wherein the cracking output shaft is disposed on the cracking mounting plate and the middle plate through a bearing, a first gear is sleeved on the cracking output shaft, and a second gear engaged with the first gear is disposed at an output end of the cracking motor;

the cover is equipped with the second and resets on the schizolysis output shaft, the second reset the one end with the schizolysis mounting panel butt, the other end with the terminal surface butt of first gear.

In combination with the first aspect, embodiments of the present invention provide a possible implementation manner of the first aspect, wherein an electromagnet is disposed on the middle plate, and the electromagnet is used for providing a magnetic field for the biochip.

In combination with the first aspect, an embodiment of the present invention provides a possible implementation manner of the first aspect, wherein a temperature control assembly and a temperature sensor are disposed on a lower surface of the middle plate, and provide a temperature environment for the PCR chamber.

With reference to the first aspect, an embodiment of the present invention provides a possible implementation manner of the first aspect, wherein the temperature control assembly includes a semiconductor cooling plate, a PCR heat conduction block, and a heat conduction block pressing plate;

the semiconductor refrigeration piece, the PCR heat conduction block and the heat conduction block pressing plate are sequentially arranged on the lower surface of the middle plate from top to bottom.

With reference to the first aspect, an embodiment of the present invention provides a possible implementation manner of the first aspect, wherein a warehouse entering tray is disposed on the bottom plate, and a guide groove for guiding the warehouse entering tray is formed on the bottom plate;

and the bottom plate is provided with an in-out driving assembly for driving the in-out bin tray to move.

In combination with the first aspect, an embodiment of the present invention provides a possible implementation manner of the first aspect, wherein, a movable base for bearing the test card is provided on the above-mentioned inlet and outlet tray, a plurality of guide posts are provided on the inlet and outlet tray, the movable base is sleeved on the guide posts, just a first reset spring is sleeved on the guide posts, one end of the first reset spring is connected with the movable base butt, and the other end is connected with the inlet and outlet tray butt.

In combination with the first aspect, embodiments of the present invention provide a possible implementation manner of the first aspect, wherein a probe assembly for reading the test data of the test card is disposed on the bottom plate.

In combination with the first aspect, embodiments of the present invention provide a possible implementation manner of the first aspect, wherein the probe assembly includes a probe mounting plate disposed on the upper surface of the bottom plate, and a plurality of retractable probes are disposed on the probe mounting plate.

In combination with the first aspect, an embodiment of the present invention provides a possible implementation manner of the first aspect, wherein the microfluidic chip nucleic acid detection apparatus further includes a liquid level detection apparatus, the liquid level detection apparatus employs a correlation type photoelectric switch, and a transmitting tube and a receiving tube of the photoelectric switch are respectively fixed on the middle plate and the bottom plate;

the light emitted by the emitting tube can penetrate through a liquid monitoring area arranged on the test card to reach the receiving tube.

In combination with the first aspect, embodiments of the present invention provide a possible implementation manner of the first aspect, wherein the valve driving assembly is installed below the bottom plate.

Has the advantages that:

the embodiment of the utility model provides a micro-fluidic chip nucleic acid detection device, which comprises a top plate, a bottom plate and a movable middle plate; a guide piece is arranged between the top plate and the bottom plate, one end of the guide piece is connected with the top plate, the other end of the guide piece is connected with the bottom plate, and the intermediate plate is arranged on the guide piece; the testing card is provided with a biochip, a PCR (polymerase chain reaction) chamber, a piston chamber, a cracking chamber and a plurality of accommodating chambers, the biochip, the cracking chamber and the accommodating chambers are communicated with the piston chamber through runners, a piston is arranged in the piston chamber, and the valve driving assembly is used for controlling the opening and closing of valves in each runner; the top plate is provided with a displacement assembly for driving the middle plate to move up and down, the top plate is also provided with a piston driving assembly capable of moving up and down, and the piston driving assembly is used for driving a piston to move; the middle plate is provided with a cracking component which can drive the cracking component in the cracking chamber to rotate.

When using, reciprocate along the guide through displacement assembly drive intermediate lamella to make the schizolysis subassembly on the intermediate lamella can drive the schizolysis part on the test card and carry out the schizolysis work, can drive the piston on the test card through the piston drive assembly on the roof and carry out work, and through valve drive assembly's cooperation, make the chamber that holds, biochip, PCR chamber or schizolysis chamber and the piston chamber intercommunication of each different functions, thereby carry out corresponding work. Therefore, the work of nucleic acid extraction, amplification, detection and the like can be performed on the test card, the continuity of each flow in nucleic acid detection is increased, the operation steps of personnel are simplified, and the work efficiency is improved.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments or the technical solutions in the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

Fig. 1 is a schematic diagram of an overall structure of a microfluidic chip nucleic acid detection device according to an embodiment of the present invention;

fig. 2 is an exploded view of a microfluidic chip nucleic acid detection device according to an embodiment of the present invention;

fig. 3 is an exploded view of a top plate portion of a microfluidic chip nucleic acid detecting apparatus according to an embodiment of the present invention;

fig. 4 is an exploded view of a middle plate portion of a microfluidic chip nucleic acid detecting apparatus according to an embodiment of the present invention;

fig. 5 is a schematic diagram of a lysis component in a microfluidic chip nucleic acid detection device according to an embodiment of the present invention;

fig. 6 is a schematic diagram illustrating a connection between a cleavage output end and a cleavage portion of a test card in the microfluidic chip nucleic acid detecting apparatus according to the embodiment of the present invention;

FIG. 7 is an exploded view of a bottom plate of a microfluidic chip nucleic acid detecting device according to an embodiment of the present invention;

fig. 8 is a schematic view of an in-out driving assembly in a microfluidic chip nucleic acid detecting apparatus according to an embodiment of the present invention;

fig. 9 is a schematic view of a movable base in the microfluidic chip nucleic acid detection device according to an embodiment of the present invention;

FIG. 10 is a schematic view of a liquid level detection device in the microfluidic chip nucleic acid detection device according to an embodiment of the present invention;

fig. 11 is an exploded view of a piston driving assembly in the microfluidic chip nucleic acid detecting device according to an embodiment of the present invention;

fig. 12 is an exploded view of a driving rod in a piston driving assembly of a microfluidic chip nucleic acid detecting device according to an embodiment of the present invention;

fig. 13 is a schematic diagram of a piston in the microfluidic chip nucleic acid detecting apparatus according to an embodiment of the present invention;

fig. 14 is a schematic view of a valve driving assembly in a microfluidic chip nucleic acid detecting device according to an embodiment of the present invention;

fig. 15 is an illustration of the principle of use of the microfluidic chip nucleic acid detection device according to the embodiment of the present invention.

Icon:

100-a top plate; 101-a guide; 102-a cam motor; 103-a camshaft; 104-a cam; 105-a first reset piece; 106-camshaft mounting plate; 107-cam follower; 108-a first microswitch; 109-a first feeler lever; 110-screw motor; 111-lead screw; 112-a piston drive;

200-a middle plate; 201-a lysis motor; 202-a cleavage output shaft; 203-lysis mounting plate; 204-support column; 205-a first gear; 206-a second gear; 207-a second reset piece; 208-an electromagnet; 209-linear bearing; 210-a temperature sensor; 211-semiconductor chilling plates; 212-PCR heat conducting block; 213-heat conducting block pressing plate; 214-a color sensor;

300-a base plate; 301-mounting a bracket; 302-warehouse in and out tray; 303-a movable base; 304-a guide post; 305-a first return spring; 306-a probe mounting plate; 307-probe; 308-a launch tube; 309-receiving tube; 310-a bin inlet and outlet motor; 311-a mount; 312 — a synchronizing shaft; 313-a drive gear; 314-a bevel gear; 315-rack; 316-third return spring; 317-ejector pin; 318-gas valve; 319-air valve mounting plate; 320-airbag soft membrane; 321-an air valve line concentration board;

400-test card; 401-a piston; 402-a lysis member; 403-fluid monitoring zone.

Detailed Description

The technical solution of the present invention will be described clearly and completely with reference to the accompanying drawings, and obviously, the described embodiments are some, but not all embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", and the like, indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

The present invention will be described in further detail below with reference to specific embodiments and with reference to the attached drawings.

Referring to fig. 1 to 12, the present embodiment provides a microfluidic chip nucleic acid detecting apparatus, including a top plate 100, a bottom plate 300, and a movable middle plate 200; a guide 101 is arranged between the top plate 100 and the bottom plate 300, one end of the guide 101 is connected with the top plate 100, the other end is connected with the bottom plate 300, and the middle plate 200 is arranged on the guide 101; the bottom plate 300 is provided with a test card 400 and a valve driving assembly, the test card 400 is provided with a biochip, a PCR (Polymerase Chain Reaction) chamber, a piston chamber, a cracking chamber and a plurality of accommodating chambers, the biochip, the cracking chamber and the accommodating chambers are all communicated with the piston chamber through runners, a piston 401 is arranged in the piston chamber, and the valve driving assembly is used for controlling opening and closing of valves in each runner; a displacement assembly for driving the middle plate 200 to move up and down is arranged on the top plate 100, and a piston driving assembly capable of moving up and down is also arranged on the top plate 100 and is used for driving the piston 401 to move; the middle plate 200 is provided with a cracking assembly capable of driving the cracking part 402 in the cracking chamber to rotate.

When using, reciprocate along guide 101 through displacement assembly drive intermediate lamella 200 to make the schizolysis subassembly on the intermediate lamella 200 can drive the schizolysis part 402 on the test card 400 and carry out the schizolysis work, can drive piston 401 on the test card 400 through the piston drive assembly on the roof 100 and carry out work, and the cooperation through valve drive assembly, make the chamber that holds of each different function, biochip, PCR chamber or schizolysis chamber and piston chamber intercommunication, thereby carry out corresponding work. Therefore, the work of nucleic acid extraction, amplification, detection and the like can be carried out on the test card 400, the continuity of each process in nucleic acid detection is increased, the operation steps of personnel are simplified, the whole reaction system is totally closed relative to the external environment, the pollution is effectively avoided, and the requirement on the detection environment is reduced.

The guide 101 may be an optical axis.

Referring to fig. 3, in an alternative of the present embodiment, the displacement assembly includes a cam motor 102, a cam shaft 103, a cam 104, and a first restoring member 105; the first reset piece 105 is sleeved on the guide piece 101, one end of the first reset piece 105 is abutted with the bottom plate 300, and the other end of the first reset piece 105 is abutted with the middle plate 200; a cam shaft mounting plate 106 is arranged on the lower surface of the top plate 100, the cam motor 102 is in transmission connection with the cam shaft 103, the cam 104 is mounted on the cam shaft 103, and a cam follower 107 matched with the cam 104 is arranged on the middle plate 200; the camshaft mounting plate 106 is provided with a first microswitch 108, and the camshaft 103 is provided with a first contact rod 109 for triggering the first microswitch 108.

Wherein, the first reset piece 105 may adopt a second reset spring. The cam motor 102 may employ a stepping motor.

Referring to fig. 3, in an alternative of this embodiment, the piston drive assembly includes a lead screw motor 110, a lead screw 111, and a piston drive 112; the lead screw motor 110 is connected with a lead screw 111, and a lead screw nut adapted to the lead screw 111 is fixedly arranged on the piston driving member 112.

The lead screw motor 110 may be a stepping motor.

The top plate 100 and the bottom plate 300 are fixedly connected through four optical axes, the intermediate plate 200 is sleeved on the four optical axes and can move up and down under the driving of the displacement assembly, a second return spring is abutted between the intermediate plate 200 and the bottom plate 300, and meanwhile, the cam followers 107 arranged on the intermediate plate 200 are respectively abutted with the cams 104 arranged on the top plate 100, so that the rotation of the cams 104 can control the intermediate plate 200 to move up and down. Four linear bearings 209 are fixedly arranged on the middle plate 200 and are used for being sleeved on the optical axis during installation, so that friction between the middle plate 200 and the optical axis is reduced; cam followers 107 corresponding to the cams 104 are respectively installed on the left and right side surfaces of the intermediate plate 200 and are coaxially arranged, and can move the intermediate plate 200 up and down with the rotation of the cams 104.

The lead screw motor 110 is fixed on the top plate 100 through a screw, and a lead screw nut of the lead screw motor 110 is fixed on the piston driving member 112, so that the piston driving member 112 can perform controllable up-and-down movement under the driving of the lead screw motor 110, and when the detection work is performed, the piston driving member 112 is used for connecting the piston 401 on the test card 400, thereby driving the piston 401 to move.

The cam motor 102 is fixed on the top plate 100 through a connecting plate, meanwhile, a gear fixed on an output shaft of the cam motor 102 is meshed with a gear fixed on the cam shaft 103, the cam shaft 103 is fixed on the top plate 100 through a cam shaft mounting plate 106, the cam shaft mounting plate 106 is fixed on the top plate 100 through a positioning pin, two ends of the cam shaft 103 are sleeved on the cam shaft mounting plate 106 through bearings, and cams 104 are fixedly arranged at two ends of the cam shaft 103 extending out of the cam shaft mounting plate 106, wherein the two cams 104 are identical in mounting direction and synchronous in angle, and a first contact rod 109 fixed on the cam shaft 103 is used for triggering a first microswitch 108 fixed on the cam shaft mounting plate 106 when the cams 104 rotate to a specific angle so as to output a movement; for example, when the cam 104 is rotated in a first direction by a certain angle to activate the first microswitch 108, it indicates that the middle layer is lowered to the maximum height, and when the cam 104 is rotated in a second direction by a certain angle to activate the first microswitch 108, it indicates that the middle layer is raised to the maximum height.

It should be noted that the top plate 100 is provided with a stepping motor driver for controlling the operation of the stepping motor.

Referring to fig. 5 and 6, in an alternative of the present embodiment, the cracking assembly includes a cracking motor 201, a cracking output shaft 202 and a cracking mounting plate 203; the cracking mounting plate 203 is arranged on the middle plate 200 through a support column 204, the cracking motor 201 is arranged on the cracking mounting plate 203, and the cracking output shaft 202 is in transmission connection with the cracking motor 201; one end of the cleavage output shaft 202 passes through and protrudes from the intermediate plate 200.

Referring to fig. 5, in an alternative of this embodiment, a cracking output shaft 202 is disposed on a cracking mounting plate 203 and an intermediate plate 200 through bearings, a first gear 205 is sleeved on the cracking output shaft 202, and an output end of a cracking motor 201 is provided with a second gear 206 meshed with the first gear 205; the cracking output shaft 202 is sleeved with a second reset piece 207, one end of the second reset piece 207 is abutted to the cracking mounting plate 203, and the other end of the second reset piece is abutted to the end face of the first gear 205.

Wherein, the second reset piece 207 adopts a spring.

The cracking motor 201 is installed on the cracking installation plate 203, the cracking installation plate 203 is connected with the middle plate 200 through three support columns 204, a first bearing is installed in a through hole on the cracking installation plate 203, a second bearing is installed in a stepped hole arranged on the middle plate 200, the cracking output shaft 202 is simultaneously sleeved in two bearings, a spring and a gasket are sleeved at the upper end of the cracking output shaft 202, the bearings, the gasket, the spring and the cracking output shaft 202 are sequentially abutted, the output end of the cracking output shaft 202 penetrates through the stepped hole on the middle plate 200 to be exposed to the bottom surface of the middle plate 200, the cracking output shaft 202 can move upwards for a certain distance along the axial direction when the output end is subjected to external force, the end part of the output end of the cracking output shaft 202 is of a cross structure, a groove structure matched with the cracking rotor on the test card 400 is arranged on the cracking rotor, and the initial rotation, the design that schizolysis output shaft 202 is telescopic can avoid the schizolysis rotor to collide when not matching with schizolysis output shaft 202 initial position, when the output shaft did not match with the schizolysis rotor, schizolysis output shaft 202 received external force and can retract, after schizolysis output shaft 202 rotated certain angle, schizolysis output shaft 202 was automatic when the schizolysis rotor matches with the output shaft angle stretches out and gets into the matching recess on the schizolysis rotor, and schizolysis output shaft 202 can drive the inside synchronous rotation of schizolysis rotor of test card 400 this moment.

Referring to fig. 4, in an alternative embodiment, the middle plate 200 is provided with an electromagnet 208, and the electromagnet 208 is used for providing a magnetic field for the biochip.

The electromagnet 208 is fixed on the upper surface of the middle plate 200, has a magnetic pole penetrating through the middle plate 200 and exposed to the lower surface of the middle plate 200, and has a magnetic pole positioned directly above the biochip on the test card 400 for providing a magnetic field environment of a specific intensity to the biochip when testing.

Referring to fig. 4, in an alternative embodiment, a temperature control assembly and a temperature sensor 210 are disposed on the lower surface of the middle plate 200 to provide a temperature environment for the PCR chamber.

Referring to fig. 4, in an alternative embodiment, the temperature control assembly includes a semiconductor cooling plate 211, a PCR heat-conducting block 212, and a heat-conducting block pressing plate 213; the semiconductor chilling plates 211, the PCR heat-conducting blocks 212, and the heat-conducting block pressing plates 213 are sequentially disposed on the lower surface of the middle plate 200 from top to bottom.

The semiconductor refrigeration piece 211 is fixed in the installation groove on the bottom surface of the middle plate 200, the semiconductor refrigeration piece 211, the PCR heat conduction block 212 and the heat conduction block pressing plate 213 are sequentially abutted, and the PCR heat conduction block 212 is provided with a protruding part which protrudes out of the heat conduction block pressing plate 213 and contacts with a PCR chamber reaction area arranged on the test card 400 so as to control the temperature change of the PCR chamber reaction area on the test card 400.

Referring to fig. 7, in an alternative of this embodiment, a bottom plate 300 is provided with a warehouse in/out tray 302, and the bottom plate 300 is provided with a guide groove for guiding the warehouse in/out tray 302; an in-out driving assembly for driving the in-out bin tray 302 to move is provided on the bottom plate 300.

The left and right sides of the bottom plate 300 are respectively provided with a mounting bracket 301, and the mounting brackets 301 can fix the bottom plate 300 on other devices or other supporting members.

Referring to fig. 7 and 9, in an alternative of this embodiment, a movable base 303 for carrying a test card 400 is disposed on the in-out tray 302, a plurality of guide posts 304 are disposed on the in-out tray 302, the movable base 303 is sleeved on the guide posts 304, a first return spring 305 is sleeved on the guide posts 304, one end of the first return spring 305 abuts against the movable base 303, and the other end abuts against the in-out tray 302.

The bottom plate 300 is provided with a guide groove, the in-out tray 302 is arranged in the guide groove, and the in-out tray 302 can move along the groove depth direction, so as to realize the actions of receiving the test card 400 when the test is started and sending out the test card 400 after the test is finished.

The movable base 303 is arranged on the in-out bin tray 302, the movable base 303 is sleeved on four guide posts 304 fixed on the in-out bin tray 302, a first reset spring 305 is connected between the movable base 303 and the in-out bin tray 302 in a butting mode, and the test card 400 is placed on the movable base 303 during use, so that the test card 400 can move downwards along the direction of the guide posts 304 for a certain distance together with the movable base 303 when being subjected to downward pressure caused by the cracking component and the middle plate 200.

The in-out driving component comprises an in-out motor 310, a mounting seat 311 of the in-out motor 310 is fixed on the bottom plate 300, the in-out motor 310 is fixed on the mounting seat 311, a transverse synchronizing shaft 312 is arranged on the mounting seat 311, a transmission gear 313 and a bevel gear 314 are sequentially arranged on the synchronizing shaft 312, the power of the in-out motor 310 is transmitted to the synchronizing shaft 312 through the transmission gear 313, and the bevel gears 314 with two opposite tooth surfaces fixed on the synchronizing shaft 312 are respectively meshed with racks 315 on two sides of the in-out tray 302, so that the in-out tray 302 can be driven to move.

Referring to fig. 7, in an alternative embodiment, a probe 307 assembly for reading test data of the test card 400 is provided on the base plate 300.

Referring to fig. 7, in an alternative embodiment, the probe 307 assembly includes a probe mounting plate 306 disposed on the upper surface of the base plate 300, and a plurality of retractable probes 307 are disposed on the probe mounting plate 306.

A probe mounting plate 306 is disposed on the upper surface of the base plate 300, and a plurality of retractable probes 307 are disposed on the probe mounting plate 306 for electrically communicating with the biochip on the test card 400, thereby obtaining a measurement result of the biochip.

Referring to fig. 10, in an alternative of this embodiment, the microfluidic chip nucleic acid detecting apparatus further includes a liquid level detecting apparatus, the liquid level detecting apparatus employs a correlation type photoelectric switch, and a transmitting tube 308 and a receiving tube 309 of the photoelectric switch are respectively fixed on the middle plate 200 and the bottom plate 300; the light emitted from the emitting tube 308 can penetrate the liquid monitoring region 403 disposed on the test card 400 to reach the receiving tube 309.

Referring to fig. 7, in an alternative embodiment, the valve actuator assembly is mounted below the base plate 300.

The valve driving assembly comprises a third return spring 316, a push rod 317 and a gas valve 318; the third return spring 316 is sleeved on the ejector rod 317, the third return spring 316 and the ejector rod 317 are arranged in the stepped hole in the lower surface of the bottom plate 300, and two elastic ends of the third return spring 316 are respectively abutted against the step of the stepped hole of the bottom plate 300 and the ejector rod 317; the air valve mounting plate 319 is fixed on the lower surface of the bottom plate 300 through screws, a groove or a through hole is arranged on the air valve mounting plate 319 as an air path, and the air bag soft membrane 320 is clamped between the bottom plate 300 and the air valve mounting plate 319; a plurality of air valves 318 are mounted on the lower surface of the air valve mounting plate 319 and are communicated with corresponding air passages on the air valve mounting plate 319; the air valve line concentration board 321 is arranged below the air valve 318 and is connected with the air valve mounting board 319 through a plurality of support columns 204 for connecting control lines of the air valve.

Referring to fig. 14, the bottom plate 300, the airbag soft membrane 320 and the air valve mounting plate 319 are sequentially arranged in an abutting manner, and the air valve mounting plate 319 is fixed on the bottom plate 300 through bolts and clamps the airbag soft membrane 320, so that the third groove 61 on the air valve mounting plate 319 and the airbag soft membrane 320 form a closed third flow channel; a stepped hole is formed in the bottom plate 300, the ejector rod 317 is sleeved inside the stepped hole, the ejector rod 317 can penetrate out of the bottom plate 300 and is exposed on the upper surface of the bottom plate 300, and meanwhile, a third return spring 316 is abutted between the bottom plate 300 and the ejector rod 317, so that the bottom of the ejector rod 317 is abutted against the airbag soft membrane 320; the air valve 318 is fixed on one side of the air valve mounting plate 319, which is far away from the air bag soft film 320, and the air outlet of the air valve 318 is communicated with the bottom of the air bag soft film 320, which is just opposite to the ejector rod 317, through the third groove 61; when the air valve 318 is started, compressed air flows to the bottom of the air bag soft membrane 320 through the third flow channel on the air valve mounting plate 319, the air bag soft membrane 320 deforms due to the pressure difference on the two sides, the ejector rod 317 is pushed to move upwards, at the moment, the third return spring 316 is further compressed, the ejector rod 317 extends out of a preset stroke through the opening of the bottom plate 300, the extending part of the ejector rod 317 is a pressure output end, and the pressure output end enables the first channel sealing membrane 13 to abut against the test card 400, so that the through hole 12 is sealed, and the first flow channel and the second flow channel are blocked; when the first and second closed flow channels need to be opened again, only the air valve 318 needs to be closed, at this time, the bottom of the air bag soft membrane 320 is communicated with the atmosphere through the waste gas port, the pressure difference on the two sides of the air bag soft membrane 320 disappears, the push rod 317 is reset downwards due to the thrust of the third reset spring, the extending end of the push rod 317 retracts, the pressure applied to the first channel sealing membrane 13 disappears, the first channel sealing membrane 13 resets due to the elasticity of the first channel sealing membrane 13, and at this time, the first closed flow channel on the test card is communicated with the second flow channel again. The first flow channel is composed of a first channel sealing film 13 and a groove on the test card 400, and the second flow channel is composed of a second channel sealing film 11 and a groove on the test card 400.

In addition, a color sensor 214 is also included. The color sensor 214 is installed on the lower surface of the middle plate 200 and faces the piston 401 on the test card 400, the color sensor 214 is used for detecting a mark point preset on the piston 401 on the test card 400 to judge the placement state of the test card 400, and if the color sensor 214 can detect the mark point, the test card 400 is installed in place and can perform subsequent work; if the color sensor 214 fails to detect the marker, it indicates that the test card 400 is not in place.

The operation process example of the microfluidic chip nucleic acid detection device provided by the embodiment is as follows:

the in-out motor 310 drives the in-out tray 302 to extend to a preset stroke, so that an operator can conveniently place the test card 400 filled with a sample on the movable base 303 on the in-out tray 302, after the operator finishes placing, the in-out tray 302 is retracted until the test card 400 reaches a specified position between the middle plate 200 and the bottom plate 300, the position can enable the cracking component 402 (stirring paddle) on the test card 400 to be positioned right below the cracking output shaft 202 of the cracking component on the middle plate 200, meanwhile, the metal contact of the Printed Circuit Boards (PCBs) on the biochips loaded on the test card 400 is positioned right above the telescopic probes 307 on the probe mounting plate 306, and at this time, the cracking output shaft 202 of the cracking component and the stirring paddle of the test card 400, and the PCBs on the test card 400 and the telescopic probes 307 are in a non-contact state; after the test card 400 reaches a specified position, the cam motor 102 located on the top plate 100 drives the cam 104 to rotate by a specified angle, because the cam follower 107 fixed on the middle plate 200 abuts against the working surface of the cam 104, the middle plate 200 moves downwards by a set distance, in the process of downward movement of the middle plate 200, the upper surface of the test card 400 abuts against the lower surface of the middle plate 200 and moves downwards along with the middle plate 200, the connection between the cracking output shaft 202 and the cracking part 402 of the test card 400 (the cracking part 402 can adopt a cracking rotor), the PCB on the test card 400 and the telescopic probe 307 is completed while moving downwards, when the middle plate 200 moves downwards to a terminal point, the first return spring 305 on the movable base 303 is pressed, and the test card 400 is pressed and fixed; after the compressing action is completed, the screw motor 110 on the top plate 100 drives the piston driver 112 to move downwards, and the piston driver 112 can be fixedly connected with the piston 401 on the test card 400, and the piston 401 on the test card 400 can be controlled by the screw motor 110 to move upwards and downwards, so that the volume of the piston chamber on the test card 400 is changed, and the liquid in the test card 400 is driven to move in a controlled manner.

As shown in fig. 11-13, the large end 271 of the positioning post on the driving rod 21 is opposite to the large end 82 of the arc-shaped slot on the piston 401, then the linear driving device drives the driving rod 21 to move toward the direction close to the piston rod 8 until the large end 271 of the positioning post passes through the large end 82 of the arc-shaped slot on the piston rod 8, at this time, the positioning pressing plate 26 abuts against the piston rod 8, the thrust spring 25 is compressed, then the motor 5 rotates, the motor 5 drives the driving rod 21 to rotate around the axis until the second contact rod 23 triggers the third micro switch 7, because the small end 272 of the positioning post is on the same plane with the arc-shaped slot of the piston rod 8, the small end 272 of the positioning post can be smoothly screwed into the small end 81 of the arc-shaped slot of the piston rod 8, and finally the driving rod 21 moves toward the direction far away from the piston rod 8, because the width of the small end slot on the piston rod 8 is smaller, and because the positioning pressing plate 26 is abutted with the thrust spring 25, the positioning pressing plate 26 presses the piston push rod 8 against the side surface of the large end 271 of the positioning column; so far, the connection process is completed, and the connection is released only by reverse operation according to the process, and it should be noted that the micro switch which needs to be triggered when the driving rod 21 rotates when the connection is released is the second micro switch 6.

Referring to fig. 15, fig. 15 is a structure diagram of a fluid network inside a test card 400, which is taken as an example to further illustrate an operation mechanism of the apparatus, and the fluid network structure is not limited to the following diagram form, and can be flexibly changed according to different detection processes and detection principles: the air valves 318 on the bottom plate 300 of the device correspond to V01-V14 in the figure, when in use, the fluid channels can be gated by controlling the switches of the air valves 318, so as to control the flow direction of the fluid; for example, when a quantitative sample needs to be extracted and transferred to the lysis chamber, the other air valves except the V03 air valve need to be opened (note that since the microchannel valve on the test card 400 is in a normally open state, the state of the air valve 318 is opposite to the state of the microchannel valve, that is, when the air valve 318 is turned on, the corresponding microchannel valve is closed, and when the air valve 318 is closed, the corresponding microchannel valve is opened), then the piston 401 is driven to move for a set distance in a direction that increases the cavity volume of the piston 401, so that the quantitative sample can be extracted into the piston chamber, then the air valves except the V13 are opened, and finally the piston 401 is driven to move for a set distance in a direction that decreases the cavity volume of the piston 401, so that the sample can be transferred to the lysis chamber. The steps of the nucleic acid extraction and nucleic acid amplification process are complicated, and those skilled in the reaction process know that the solution transfer, dilution, mixing, adsorption, cleaning and other operations in the reaction can be completed by using the device only by matching with the actions of the control air valve 318, the driving piston 401 and the like, so that the details are not repeated herein; the amplified sample is transferred to a biochip through a microchannel for detection, a liquid level detection device is arranged at the front end of a flow channel where the biochip is located, the liquid level detection device is a correlation photoelectric switch, a transmitting tube 308 and a receiving tube 309 of the photoelectric switch are respectively fixed on an intermediate plate 200 and a bottom plate 300, light of the transmitting tube 308 penetrates through a liquid monitoring area 403 arranged on a test card 400 and reaches the receiving tube 309, and errors possibly existing in all previous motion steps are eliminated by detecting the change of light transmittance when liquid exists or not so as to obtain the actual position of the front end of the fluid, so that the sample to be detected can accurately reach the surface of the biochip. The microfluidic chip nucleic acid detection device provided by the embodiment realizes integration and automation of nucleic acid extraction, amplification and detection, simplifies the personnel operation steps, and effectively avoids pollution and reduces the requirement on the environment because the whole reaction system is totally closed relative to the external environment.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the embodiments of the present invention.

Claims (14)

1. A microfluidic chip nucleic acid detection device, comprising: a top plate (100), a bottom plate (300) and a movable intermediate plate (200);

a guide (101) is arranged between the top plate (100) and the bottom plate (300), one end of the guide (101) is connected with the top plate (100), the other end of the guide (101) is connected with the bottom plate (300), and the middle plate (200) is arranged on the guide (101);

the testing device comprises a bottom plate (300), a testing card (400) and a valve driving assembly, wherein the testing card (400) is provided with a biochip, a PCR (polymerase chain reaction) chamber, a piston chamber, a cracking chamber and a plurality of accommodating chambers, the biochip, the cracking chamber and the accommodating chambers are communicated with the piston chamber through runners, a piston (401) is arranged in the piston chamber, and the valve driving assembly is used for controlling the opening and closing of valves in each runner;

the top plate (100) is provided with a displacement assembly for driving the middle plate (200) to move up and down, the top plate (100) is also provided with a piston driving assembly capable of moving up and down, and the piston driving assembly is used for driving the piston (401) to move;

the middle plate (200) is provided with a cracking component capable of driving a cracking component (402) in the cracking chamber to rotate.

2. The microfluidic chip nucleic acid detection device according to claim 1, wherein the displacement assembly comprises a cam motor (102), a cam shaft (103), a cam (104) and a first reset piece (105);

the first resetting piece (105) is sleeved on the guide piece (101), one end of the first resetting piece (105) is abutted against the bottom plate (300), and the other end of the first resetting piece (105) is abutted against the middle plate (200);

a camshaft mounting plate (106) is arranged on the lower surface of the top plate (100), the cam motor (102) is in transmission connection with the camshaft (103), the cam (104) is mounted on the camshaft (103), and a cam follower (107) matched with the cam (104) is arranged on the middle plate (200);

the camshaft installing plate (106) is provided with a first microswitch (108), and the camshaft (103) is provided with a first contact rod (109) used for triggering the first microswitch (108).

3. The microfluidic chip nucleic acid detecting device according to claim 1, wherein the piston driving assembly comprises a lead screw motor (110), a lead screw (111) and a piston driving member (112);

the screw motor (110) is connected with the screw (111), and a screw nut matched with the screw (111) is fixedly arranged on the piston driving piece (112).

4. The microfluidic chip nucleic acid detection device of claim 1, wherein the lysis component comprises a lysis motor (201), a lysis output shaft (202), and a lysis mounting plate (203);

the cracking mounting plate (203) is mounted on the middle plate (200) through a support column (204), the cracking motor (201) is mounted on the cracking mounting plate (203), and the cracking output shaft (202) is in transmission connection with the cracking motor (201);

one end of the cracking output shaft (202) penetrates through and protrudes out of the middle plate (200).

5. The microfluidic chip nucleic acid detection device according to claim 4, wherein the lysis output shaft (202) is arranged on the lysis mounting plate (203) and the middle plate (200) through bearings, a first gear (205) is sleeved on the lysis output shaft (202), and a second gear (206) meshed with the first gear (205) is arranged at an output end of the lysis motor (201);

the cover is equipped with the second and resets piece (207) on schizolysis output shaft (202), the second reset the one end of piece (207) with schizolysis mounting panel (203) butt, the other end with the terminal surface butt of first gear (205).

6. The microfluidic chip nucleic acid detecting device according to claim 1, wherein an electromagnet (208) is disposed on the middle plate (200), and the electromagnet (208) is used for providing a magnetic field for the biochip.

7. The microfluidic chip nucleic acid detection device according to claim 1, wherein the lower surface of the middle plate (200) is provided with a temperature control assembly and a temperature sensor (210) for providing a temperature environment for the PCR chamber.

8. The microfluidic chip nucleic acid detection device according to claim 7, wherein the temperature control assembly comprises a semiconductor cooling plate (211), a PCR heat conducting block (212) and a heat conducting block pressing plate (213);

the semiconductor refrigeration sheet (211), the PCR heat conduction block (212) and the heat conduction block pressing plate (213) are sequentially arranged on the lower surface of the middle plate (200) from top to bottom.

9. The microfluidic chip nucleic acid detection device according to claim 1, wherein the bottom plate (300) is provided with a warehouse inlet and outlet tray (302), and the bottom plate (300) is provided with a guide groove for guiding the warehouse inlet and outlet tray (302);

and an in-out driving assembly for driving the in-out bin tray (302) to move is arranged on the bottom plate (300).

10. The microfluidic chip nucleic acid detection device according to claim 9, wherein a movable base (303) for bearing the test card (400) is arranged on the in-out tray (302), a plurality of guide posts (304) are arranged on the in-out tray (302), the movable base (303) is sleeved on the guide posts (304), a first return spring (305) is sleeved on the guide posts (304), one end of the first return spring (305) abuts against the movable base (303), and the other end of the first return spring abuts against the in-out tray (302).

11. The microfluidic chip nucleic acid detecting apparatus according to claim 1, wherein the bottom plate (300) is provided with a probe (307) assembly for reading the test data of the test card (400).

12. The microfluidic chip nucleic acid detecting apparatus according to claim 11, wherein the probe (307) assembly includes a probe mounting plate (306) disposed on an upper surface of the base plate (300), and the probe mounting plate (306) is provided with a plurality of retractable probes (307).

13. The microfluidic chip nucleic acid detection device according to claim 1, further comprising a liquid level detection device, wherein the liquid level detection device employs a correlation type photoelectric switch, and a transmitting tube (308) and a receiving tube (309) of the photoelectric switch are respectively fixed on the middle plate (200) and the bottom plate (300);

the light emitted by the emitting tube (308) can penetrate through a liquid monitoring area (403) arranged on the test card (400) to reach the receiving tube (309).

14. The microfluidic chip nucleic acid detecting device according to claim 1, wherein the valve driving assembly is installed below the base plate (300).

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