CN113699027A - Piston lifting and sealing membrane puncturing device of full-automatic PCR analysis system - Google Patents
Piston lifting and sealing membrane puncturing device of full-automatic PCR analysis system Download PDFInfo
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- CN113699027A CN113699027A CN202111000754.3A CN202111000754A CN113699027A CN 113699027 A CN113699027 A CN 113699027A CN 202111000754 A CN202111000754 A CN 202111000754A CN 113699027 A CN113699027 A CN 113699027A
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- 238000007789 sealing Methods 0.000 title claims abstract description 48
- 239000012528 membrane Substances 0.000 title claims abstract description 29
- 238000010222 PCR analysis Methods 0.000 title claims abstract description 24
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 104
- 239000007788 liquid Substances 0.000 claims abstract description 19
- 230000001960 triggered effect Effects 0.000 claims description 3
- 238000001125 extrusion Methods 0.000 claims description 2
- 238000003745 diagnosis Methods 0.000 abstract description 6
- 238000000338 in vitro Methods 0.000 abstract description 2
- 238000004458 analytical method Methods 0.000 description 22
- 238000001514 detection method Methods 0.000 description 20
- 230000003321 amplification Effects 0.000 description 19
- 238000003199 nucleic acid amplification method Methods 0.000 description 19
- 230000003287 optical effect Effects 0.000 description 13
- 239000000523 sample Substances 0.000 description 13
- 238000010438 heat treatment Methods 0.000 description 10
- 238000000034 method Methods 0.000 description 10
- 238000010586 diagram Methods 0.000 description 9
- 230000003028 elevating effect Effects 0.000 description 9
- 230000005284 excitation Effects 0.000 description 9
- 102000039446 nucleic acids Human genes 0.000 description 9
- 108020004707 nucleic acids Proteins 0.000 description 9
- 150000007523 nucleic acids Chemical class 0.000 description 9
- 239000013307 optical fiber Substances 0.000 description 9
- 230000002457 bidirectional effect Effects 0.000 description 5
- 239000000835 fiber Substances 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 238000000197 pyrolysis Methods 0.000 description 5
- 238000005336 cracking Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 230000007246 mechanism Effects 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 230000003993 interaction Effects 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 102000011931 Nucleoproteins Human genes 0.000 description 1
- 108010061100 Nucleoproteins Proteins 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 238000012631 diagnostic technique Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000001821 nucleic acid purification Methods 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- 238000004227 thermal cracking Methods 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65B—MACHINES, APPARATUS OR DEVICES FOR, OR METHODS OF, PACKAGING ARTICLES OR MATERIALS; UNPACKING
- B65B69/00—Unpacking of articles or materials, not otherwise provided for
- B65B69/0033—Unpacking of articles or materials, not otherwise provided for by cutting
- B65B69/0041—Unpacking of articles or materials, not otherwise provided for by cutting by puncturing
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- General Health & Medical Sciences (AREA)
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Abstract
The invention belongs to the technical field of in-vitro diagnosis, and particularly relates to a piston lifting and sealing membrane puncturing device of a full-automatic PCR analysis system. The technical scheme is as follows: a piston lifting and sealing membrane puncturing device of a full-automatic PCR analysis system comprises a framework, a piston lifting module on the framework, a push rod for driving a rubber piston in a reagent box to move, and a push rod extending into the reagent box, wherein the output end of the piston lifting module is connected with the push rod; the reagent box is characterized in that the framework is hinged with a rotating plate, one end of the rotating plate is lapped on the output end of the piston lifting module, the other end of the rotating plate is hinged with a puncture frame, the other end of the puncture frame is connected with a plurality of puncture rods used for puncturing a liquid reagent sealing film in the reagent box, and the puncture rods extend into the reagent box. The invention provides a piston lifting and sealing membrane puncturing device of a full-automatic PCR analysis system.
Description
Technical Field
The invention belongs to the technical field of in-vitro diagnosis, and particularly relates to a piston lifting and sealing membrane puncturing device of a full-automatic PCR analysis system.
Background
The technology of separating and purifying nucleic acid is a basic technology of biochemistry and molecular biology. With the wide application of molecular biology technology in biology, medicine and related fields, the nucleic acid separation and purification technology has been further developed. The development of molecular biology is greatly facilitated by the continuous emergence of various new methods, well-established classical methods and commercial reagent methods. Nucleic acids are biomacromolecules synthesized by the polymerization of many nucleotides, and are one of the most basic substances of life. Nucleic acid is widely present in all animal and plant cells and microorganisms, and nucleic acid in organisms is often combined with protein to form nucleoprotein. With the rapid development of molecular biology in recent years, nucleic acid-based molecular diagnostic and detection techniques have increasingly highlighted important roles in a variety of fields. The existing nucleic acid purification has the advantages of short time consumption, high purification precision and automatic treatment of a large batch of experimental samples; the fluorescence PCR analysis system is a method for monitoring the reaction process of PCR in real time by adding a fluorescent group on the basis of PCR and the change of a fluorescence signal and finally carrying out quantitative analysis on an unknown template. Currently, real-time fluorescence quantitative PCR has become an authoritative method for comparing quantitative differences in gene expression levels among different samples.
Over the past decade, this approach has rapidly become popular, involving multiple areas of science. However, the conventional PCR analysis system cannot automatically puncture the sealing membrane of the liquid reagent and automatically extract the required reagent to the required position to sequentially complete the steps of nucleic acid cleavage and extraction, which results in low diagnosis efficiency.
Disclosure of Invention
In order to solve the above problems in the prior art, the present invention provides a piston lifting and sealing membrane puncturing device for a full-automatic PCR analysis system.
The technical scheme adopted by the invention is as follows:
a piston lifting and sealing membrane puncturing device of a full-automatic PCR analysis system comprises a framework, a piston lifting module on the framework, a push rod for driving a rubber piston in a reagent box to move, and a push rod extending into the reagent box, wherein the output end of the piston lifting module is connected with the push rod; the reagent box is characterized in that the framework is hinged with a rotating plate, one end of the rotating plate is lapped on the output end of the piston lifting module, the other end of the rotating plate is hinged with a puncture frame, the other end of the puncture frame is connected with a plurality of puncture rods used for puncturing a liquid reagent sealing film in the reagent box, and the puncture rods extend into the reagent box.
In the automatic operation process of the analysis system, the piston lifting module drives the push rod to lift. When the push rod descends, the push rod extends into the reagent box to push the rubber piston in the reagent box to move, so that the liquid is moved. Thereby drawing a desired reagent through the piston and extruding the discharged reagent to a desired position to automatically analyze the reagent. When the piston lifting module rises to a certain position, the rotating plate can be pushed to incline, so that the puncture frame and the puncture rod correspondingly descend. The puncture rod extends into the reagent box when being pressed downwards, and the puncture rod can puncture the liquid reagent sealing film in the reagent box, so that the reagent can flow out of the sealing film, and the reagent can be conveniently drained to a required position subsequently. The push rod and the puncture rod are driven by the same driving mechanism, and the power is transmitted by the rotating plate, so that the structure is simplified, and the movement of the push rod and the puncture rod is conveniently and accurately controlled. And the rotating plate is used for reversing, so that the push rod and the puncture rod can not synchronously move downwards all the time, the condition that the reagent is extruded out of the reagent box is avoided, and the accurate flow direction of the reagent is ensured.
As a preferred scheme of the invention, the framework is also provided with a control circuit board, and the piston lifting module is electrically connected with the control circuit board. The driving mechanisms of the push rod and the puncture rod can be controlled by the control circuit board, so that the push rod and the puncture rod can accurately act, and the processes of piston lifting and membrane sealing puncture can be automatically carried out.
As a preferred scheme of the present invention, the piston lifting module includes a lifting motor mounted on the frame, the lifting motor is electrically connected to the control circuit board, an output end of the lifting motor is connected to a lifting screw, the lifting screw is in threaded connection with a lifting plate, the push rod is connected to the lifting plate, and one end of the rotating plate is lapped on the lifting plate. The lifting motor drives the lifting screw rod to rotate, and the lifting screw rod drives the lifting plate to ascend or descend. When the lifting plate descends, the pushing on the lifting plate extends into the reagent box and drives the rubber piston in the reagent box to move, so that the required reagent can be extracted to a required position through the action of the driving piston. When the lifting motor drives the lifting plate to ascend to a certain position, the lifting plate pushes the rotating plate to incline, so that the rotating plate can press the puncture frame and the puncture rod downwards, and the puncture rod can puncture the liquid reagent sealing film in the reagent box.
As a preferable scheme of the invention, a positioning sheet is fixed on the lifting plate, a plurality of positioning sensors are arranged on the moving path of the positioning sheet, and the positioning sensors are electrically connected with the control circuit board. The positioning sensor comprises a squeezing piston position sensor, a middle position sensor and a puncture position sensor. When the positioning sheet moves to the position of a certain positioning sensor, the positioning sensor sends a signal to the control circuit board, the control circuit board controls the lifting motor to stop or start, and the moving accuracy of the lifting plate is guaranteed.
As a preferable scheme of the invention, the positioning sensor comprises a squeezing piston position sensor, a middle position sensor and a puncture position sensor; when the positioning sheet moves to the position of the extrusion piston position sensor, the push rod moves to the lowest end, when the positioning sheet moves to the position of the middle position sensor, the puncture rod and the push rod are both separated from the reagent box, and when the positioning sheet moves to the position of the puncture position sensor, the puncture rod is positioned at the position of puncturing the liquid reagent sealing membrane.
As a preferred scheme of the invention, the framework is also provided with a plurality of guide rods, guide cylinders are sleeved on the guide rods, and the guide cylinders are fixed on the lifting plate. When the lifting plate is driven by the lifting screw, the lifting plate is limited by the guide post to rotate, so that the lifting plate can stably and linearly lift.
As a preferable scheme of the invention, the number of the combination formed by the guide rods and the guide cylinders is two, and the two guide cylinders are respectively arranged on two sides of the lifting plate. When guide cylinder and guide pillar were two, the guide arm homoenergetic of both sides led guide cylinder on the lifter plate, further improved the stationarity that the lifter plate removed, avoided the lifter plate slope.
As a preferable scheme of the invention, a plurality of return springs for bouncing the puncture frame are connected to the puncture frame. After the lifting plate descends, the lifting plate is separated from the rotating plate, and the puncture frame and the puncture rod are pushed upwards by the reset spring in the resetting process, so that the puncture rod is prevented from staying in the reagent box.
As a preferable scheme of the invention, a first touch pressure sensor is arranged on the skeleton, and the first touch pressure sensor is triggered after the puncture rack is lifted. When the puncture frame rises to extrude the first touch sensor, the first touch sensor sends a signal to the control circuit board, and the control circuit board controls the lifting motor to stop.
As a preferable scheme of the invention, the skeleton is provided with a limit groove for limiting the puncture rack. The limiting groove can limit the puncture frame, and the puncture frame can be guaranteed to accurately move along a straight line, so that the puncture rod can be guaranteed to accurately puncture the sealing film.
The invention has the beneficial effects that:
the piston lifting module can drive the push rod to lift. When the push rod descends, the push rod extends into the reagent box to push the rubber piston in the reagent box to move, so that the liquid is moved. The reagent is automatically analyzed by drawing the required reagent through the piston and extruding the discharged reagent to the required position. When the piston lifting module rises to a certain position, the rotating plate can be pushed to incline, so that the puncture frame and the puncture rod correspondingly descend. The puncture rod extends into the reagent box when being pressed downwards, and the puncture rod can puncture the liquid reagent sealing film in the reagent box, so that the reagent can flow out of the sealing film, and the reagent can be conveniently drained to a required position subsequently. The push rod and the puncture rod are driven by the same driving mechanism, and the power is transmitted by the rotating plate, so that the structure is simplified, and the movement of the push rod and the puncture rod is conveniently and accurately controlled. And the rotating plate is used for reversing, so that the push rod and the puncture rod can not synchronously move downwards all the time, the condition that the reagent is extruded out of the reagent box is avoided, and the accurate flow direction of the reagent is ensured.
Drawings
FIG. 1 is a schematic diagram of the structure of a fully automatic PCR analysis system;
FIG. 2 is a schematic diagram of the structure of an analysis module;
FIG. 3 is an exploded view of an analysis module;
figure 4 is a partial block diagram of an analysis module cartridge;
FIG. 5 is a front view of a portion of the structure of the present invention;
FIG. 6 is a schematic structural view of a portion of the structure of the present invention;
figure 7 is a schematic view of the lower structure of an analysis module cartridge;
FIG. 8 is a schematic view of the structure of the kit;
FIG. 9 is a schematic structural diagram of a temperature-variable amplification module, a rotary valve driving module, a magnet lifting module and a thermal cracking module;
fig. 10 is a schematic structural view of a magnet lifting module;
FIG. 11 is a schematic diagram of a rotary valve drive module;
FIG. 12 is a schematic structural diagram of a temperature-variable amplification module;
FIG. 13 is a partial block diagram of the skeleton;
FIG. 14 is a schematic structural diagram of an optical signal emission detection module and a temperature-variable amplification module;
FIG. 15 is a partial block diagram of an optical signal emission detection module;
FIG. 16 is a sectional view of a temperature-variable amplification module.
In the figure, 1 — analysis module; 2-analysis module movement; 3-a lifting door assembly; 4-a base; 5-a human-computer interaction module; 6-a fan; 7-an interface board; 8-a foot pad; 9-indicator light board; 10-an indicator light guide post; 21-a backbone; 22-a control circuit board; 23-optical signal emission detection module; 24-a piston lifting module; 25-sealing a membrane puncture column module; 26-a temperature-variable amplification module; 27-rotary valve drive module; 28-magnet lifting module; 29-a pyrolysis module; 210-a kit; 31-a lift gate motor; 32-a lift gate screw; 33-a lifting table; 34-a lifting door; 35-positioning a stop block; 211-a limiting groove; 231-a bidirectional motor; 232-a light source assembly; 233-emission light filter wheel; 234-receive optical filter disk; 235-light-receiving cone; 236-fiber optic condenser; 237-detection probe; 238-detecting the circuit board; 239-a rotational positioning sensor; 2310-optical signal housing; 241-a lifting motor; 242-lifting screw rod; 243-lifting plate; 244-a push rod; 245-a spacer; 246-a positioning sensor; 247-a guide cylinder; 248-guide column; 251-a rotating plate; 252-a lancing frame; 253-piercing rod; 254-a return spring; 255-a first touch pressure sensor; 261-a suspended heating block; 262-Peltier; 263-module housing; 264-a heat sink; 265-a radiator fan; 271-a rotary electric machine; 272-a drive rod; 273-a positioning frame; 274-positioning plate; 275-valve position sensor; 276-a third touch pressure sensor; 281-a drive motor; 282-a magnet; 283-magnet slots; 284-a second touch pressure sensor; 2101-piston bore; 2102-puncture hole; 2103-microfluidic PCR chip; 2104-rotary valve operating orifice; 2321-light source board; 2322 — excitation light source; 2461-squeeze piston position sensor; 2462-median position sensor; 2463-puncture position sensor.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the invention and are not to be construed as limiting the invention.
As shown in fig. 5 and fig. 6, the piston lifting and membrane sealing puncturing device of the full-automatic PCR analysis system of the present embodiment includes a frame 21, a piston lifting module 24 on the frame 21, a push rod 244 for driving a rubber piston in the reagent kit 210 to move connected to an output end of the piston lifting module 24, and the push rod 244 extends into the reagent kit 210; the framework 21 is further hinged with a rotating plate 251, one end of the rotating plate 251 is lapped on the output end of the piston lifting module 24, the other end of the rotating plate 251 is hinged with a puncture frame 252, the other end of the puncture frame 252 is connected with a plurality of puncture rods 253 for puncturing a liquid reagent sealing film in the reagent box 210, and the puncture rods 253 extend into the reagent box 210.
During automated operation of the analysis system, the piston lifting module 24 drives the push rod 244 up and down. When the push rod 244 descends, the push rod 244 extends into the reagent cartridge 210, and pushes a rubber piston in the reagent cartridge 210 to move, so that the liquid is moved. Thereby drawing a desired reagent through the piston and extruding the discharged reagent to a desired position to automatically analyze the reagent. When piston lifting module 24 is lifted to a certain position, rotating plate 251 is pushed to tilt, so that puncture rack 252 and puncture rod 253 are correspondingly lowered. The piercing rod 253 extends into the reagent cartridge 210 when being pressed downwards, and the piercing rod 253 can pierce a liquid reagent sealing film in the reagent cartridge 210, so that the reagent can flow out of the sealing film, and the reagent can be conveniently drained to a required position subsequently. The push rod 244 and the puncture rod 253 of the present invention are driven by the same driving mechanism, and the power is transmitted by the rotating plate 251, so that the structure is simplified, and the movement of the push rod 244 and the puncture rod 253 can be accurately controlled. In addition, the rotating plate 251 is used for reversing, so that the push rod 244 and the puncture rod 253 can not synchronously move downwards all the time, the condition that the reagent is extruded out of the reagent box 210 is avoided, and the accurate flow direction of the reagent is ensured.
The piston lifting and film sealing puncturing device is part of a full-automatic PCR analysis system. As shown in fig. 1 to 4, the full-automatic PCR analysis system for molecular diagnosis using a microfluidic chip includes a plurality of analysis modules 1, wherein each analysis module 1 includes an analysis module core 2; the analysis module core 2 comprises a framework 21, a control circuit board 22 is arranged on the framework 21, and an optical signal emission detection module 23, a piston lifting module 24, a membrane sealing puncture column module 25, a variable temperature amplification module 26, a rotary valve driving module 27, a magnet lifting module 28 and a heating cracking module 29 which are all electrically connected with the control circuit board 22 are respectively arranged on the framework 21; the kit 210 is provided with a microfluidic PCR chip 2103, the microfluidic PCR chip 2103 is respectively inserted into the temperature-changing amplification module 26, the magnet lifting module 28 and the heating and cracking module 29, the output end of the rotary valve driving module 27 is inserted into the kit 210, the output ends of the piston lifting module 24 and the membrane sealing puncture column module 25 are both inserted into the kit 210, and the optical signal emission detection module 23 is connected with the temperature-changing amplification module 26 through an optical fiber.
It should be noted that, in order to ensure that the system of the present invention is convenient to use, the present invention further includes a base 4, the plurality of analysis modules 1 are installed in the base 4, a human-computer interaction module 5 is further installed on the base 4, and the analysis modules 1 are respectively electrically connected with the human-computer interaction module 5.
The analysis module 1 further comprises a casing, the casing is provided with a fan 6, an interface board 7, a foot pad 8, an indicator lamp panel 9 and an indicator light guide post 10, except the analysis module movement 2, and the interface board 7 is electrically connected with a control circuit board 22 in the analysis module movement 2.
As shown in fig. 8 and 4, the cartridge 210 is provided with a plunger hole 2101 and a puncture hole 2102 at the upper side. The output end of piston lifting module 24 can be inserted into piston hole 2101, and the output end of membrane sealing puncture column module 25 can be inserted into puncture hole 2102. As shown in fig. 9, the microfluidic PCR chip 2103 is inserted into the temperature-variable amplification module 26, the magnet lifting module 28 and the thermal lysis module 29, respectively. The reagent cartridge 210 is further provided with a rotary valve operating hole 2104, and the output end of the rotary valve driving module 27 is inserted into the rotary valve operating hole 2104.
When the user uses the kit, the user firstly switches on the power supply and starts the kit for testing, after the user inputs the sample and the information of the kit 210, the sample is added into the sample position of the kit 210, then the cover of the kit 210 is closed, the corresponding detection position of the analysis system is inserted, and the test is started by clicking. All subsequent detection operations are automatically completed by the analysis system, and after the test is completed, the corresponding hatch door of the analysis system is opened, and the user waits for taking out the reagent kit 210 and clicks to complete the test to close the hatch door.
In the automatic operation process of the analysis system, the rotary valve driving module 27 drives the rotary valve to the position corresponding to the reagent cartridge 210, and the membrane sealing and puncturing module 25 punctures the liquid reagent sealing membrane in the reagent cartridge 210. The piston lifting module 24 then drives the rubber piston in the cartridge 210 to move, thereby effecting movement of the liquid. The rotary valve driving module 27 then drives the rotary valve to the desired position, draws the desired reagent through the piston and rotates the rotary valve to the desired position, squeezing the piston to expel the reagent, thus reciprocating the multiple reagent reactions. The magnetic lifting module 28 and the heating and cracking module 29 are matched to complete the cracking and extracting functions of the nucleic acid, and finally the temperature-variable amplification module 26 and the optical signal emission and detection module 23 are matched to complete the amplification and real-time optical signal detection of the nucleic acid. After the kit 210 is installed in place, the control circuit board 22 controls the optical signal emission detection module 23, the piston lifting module 24, the membrane sealing puncture column module 25, the temperature-variable amplification module 26, the rotary valve driving module 27, the magnet lifting module 28 and the pyrolysis module 29 to act, thereby realizing automatic molecular diagnosis.
As shown in fig. 4, in order to ensure that the reagent kit 210 is sealed during molecular diagnosis, the frame 21 is provided with a lifting door assembly 3, the lifting door assembly 3 comprises a lifting door motor 31 installed on the frame 21, an output shaft of the lifting door motor 31 is connected with a lifting door lead screw 32, the lifting door lead screw 32 is in threaded connection with a lifting platform 33, the lifting platform 33 is rotatably connected with a lifting door 34, and the lifting door 34 is lapped on the side wall of the frame 21. The door motor 31 can drive the door screw 32 to rotate, and the door screw 32 drives the lifting platform 33 to lift, so that the lifting platform 33 drives the lifting door 34 to lift. The lift gate 34 is raised before the reagent cartridge 210 is installed, and the lift gate 34 is lowered after the reagent cartridge 210 is installed. A positioning stopper 35 for limiting the elevating table 33 is further mounted on the frame 21.
The specific structures of the piston lifting module 24 and the seal piercing column module 25 are described below:
as shown in fig. 5 and 6, the piston lifting module 24 includes a lifting motor 241 mounted on the frame 21, an output end of the lifting motor 241 is connected with a lifting screw 242, the lifting screw 242 is connected with a lifting plate 243 through a thread, the lifting plate 243 is connected with a push rod 244 for pushing a rubber piston in the reagent kit 210 to move, and the push rod 244 is inserted into a piston hole 2101 of the reagent kit 210. The elevating motor 241 drives the elevating screw 242 to rotate, and the elevating screw 242 drives the elevating plate 243 to ascend or descend. When the lifting plate 243 descends, the pushing force on the lifting plate 243 extends into the reagent box 210 and drives the rubber piston in the reagent box 210 to move, so that the required reagent can be extracted to a required position by driving the piston to act.
In order to ensure the lifting plate 243 to move stably, two guide rods are further installed on the framework 21, two guide cylinders 247 are fixed on the lifting plate 243, and the guide cylinders 247 are sleeved on the guide posts 248. When the elevating plate 243 is driven by the elevating screw 242, the elevating plate 243 is restricted by the two guide posts 248 to be rotated, and the elevating plate 243 can be smoothly linearly elevated.
The membrane sealing and piercing module 25 includes a rotating plate 251, the rotating plate 251 is hinged to the frame 21, one end of the rotating plate 251 is lapped on the lifting plate 243, the other end of the rotating plate 251 is hinged to a piercing frame 252, and the piercing frame 252 is connected with a plurality of piercing rods 253 for piercing the liquid reagent sealing membrane in the reagent box 210. When the lifting plate 243 is lifted, the rotating plate 251 is pushed to rotate, so that the puncturing rod 253 on the rotating plate 251 can puncture the liquid reagent sealing membrane in the reagent kit 210.
When puncturing rod 253 is lifted, first touch sensor 255 is attached to frame 21 in order to limit puncture rack 252. When the puncture rack 252 rises to press the first touch sensor 255, the first touch sensor 255 sends a signal to the control circuit board 22, and the control circuit board 22 controls the lifting motor 241 to stop.
A plurality of return springs 254 are coupled to piercing carriage 252 for biasing piercing carriage 252. When the lifting plate 243 is lowered, the lifting plate 243 is separated from the rotating plate 251, and the return spring 254 pushes the puncture rack 252 and the puncture rod 253 upwards in the process of returning, so that the puncture rod 253 is prevented from staying in the reagent kit 210.
A positioning plate 245 is fixed on the lifting plate 243, three positioning sensors 246 are arranged on the moving path of the positioning plate 245, and the positioning sensors 246 are electrically connected with the control circuit board 22. Position sensors 246 include squeeze piston position sensor 2461, neutral position sensor 2462, and pierce position sensor 2463. When the positioning tab 245 moves to the position of the squeeze piston position sensor 2461, the push rod 244 moves to the lowermost end and the reagent in the piston is completely squeezed out. When the positioning tab 245 moves to the neutral sensor 2462 position, the piercing rod 253 and the push rod 244 are both separated from the reagent cartridge 210. When the positioning tab 245 is moved to the puncturing position sensor 2463, the puncturing rod 253 is in a position to puncture the liquid reagent sealing membrane. The squeeze piston position sensor 2461, the middle position sensor 2462 and the puncture position sensor 2463 are all electrically connected to the control circuit board 22, and when any one of the positioning sensors 246 is triggered by the positioning piece 245, the positioning sensor 246 sends a signal to the control circuit board 22, and the control circuit board 22 controls the stop or start of the lifting motor 241.
The specific structure of the lower part of the analysis module cartridge 2 is described below:
as shown in fig. 7 to 10, the magnet lifting module 28 includes a driving motor 281 installed on the frame 21, a magnet 282 is connected to an output shaft of the driving motor 281, a magnet slot 283 is disposed on the magnet 282, and the microfluidic PCR chip 2103 passes through the magnet slot 283. When the driving motor 281 drives the magnet 282 to rise, the microfluidic PCR chip 2103 passes through the magnet slot 283, so that the separation of the magnetic beads inside the kit 210 is realized.
A second touch sensor 284 for detecting whether the magnet 282 is lowered in place is further mounted on the mounting bracket of the driving motor 281, and the second touch sensor 284 is electrically connected to the control circuit board 22. When the magnet 282 descends and presses the second touch sensor 284, the second touch sensor 284 sends a signal to the control circuit board 22, and the control circuit board 22 controls the driving motor 281 to stop.
Specifically, as shown in fig. 11, the rotary valve driving module 27 includes two rotary motors 271 mounted on the frame 21 and respectively disposed at two sides of the reagent cartridge 210, an output shaft of the rotary motor 271 is connected to a driving rod 272 for driving the rotary valves, and the driving rod 272 is inserted into the rotary valve operating hole 2104 of the reagent cartridge 210. The rotary motor 271 drives the driving rod 272 to rotate, and then the driving rod 272 drives the rotary valve to rotate to the corresponding position, thereby realizing the selection of different flow channels or reagents.
The framework 21 is further provided with a positioning frame 273, the driving rod 272 is provided with a positioning disc 274, the positioning disc 274 is provided with a plurality of positioning grooves, the positioning disc 274 is sleeved with a valve position sensor 275, and the valve position sensor 275 is arranged on the positioning frame 273. The valve position sensor 275 on the positioning frame 273 determines the open position of the rotary valve by detecting the rotation angle of the positioning plate 274, facilitating automatic control.
The third touch sensor 276 is mounted on the inside of the positioning frame 273, and when the reagent cartridge 210 is mounted and the reagent cartridge 210 presses the third touch sensor 276, the third touch sensor 276 transmits a mounting-in-place signal to the control circuit board 22.
As shown in fig. 12 and 14 to 15, the optical signal emission detection module 23 includes an optical signal housing 2310, a bidirectional motor 231 is installed on the optical signal housing 2310, one output shaft of the bidirectional motor 231 is connected to a light source assembly 232 and an emission light filter wheel 233, and the other output shaft of the bidirectional motor 231 is connected to a reception light filter wheel 234; a light receiving cone 235 is arranged beside the emitting light filter disc 233, the light receiving cone 235 is connected with the receiving end of the variable temperature amplification module 26 through an optical fiber, a fiber collecting lens 236 is arranged beside the receiving light filter disc 234, the fiber collecting lens 236 is connected with the emitting end of the variable temperature amplification module 26 through an optical fiber, a detection probe 237 is arranged on one side of the receiving light filter disc 234 away from the fiber collecting lens 236, and a detection circuit board 238 is connected to the detection probe 237. The light source assembly 232 includes a light source plate 2321, the light source plate 2321 is installed on the output shaft of the bidirectional motor 231, six excitation light sources 2322 with different wavelengths are uniformly distributed on the light source plate 2321, six emission light filters corresponding to the excitation light sources 2322 are arranged on the emission light filter disc 233, and six reception light filters corresponding to reception light after the excitation light is amplified by the temperature-varying amplification module 26 are arranged on the reception light filter disc 234.
The bi-directional motor 231 drives the light source module 232, the emission light filter disk 233 and the reception light filter disk 234 to rotate, and when an excitation light source 2322 on the light source module 232 is aligned with the light receiving cone 235, the light emitted by the light source is filtered by the emission light filter with the wavelength corresponding to the light emitting light filter, and then is transmitted to the temperature varying amplification module 26 through the light receiving cone 235. The light passes through the reagent sample in the temperature-variable amplification module 26, and the temperature of the temperature-variable amplification module 26 is kept stable between ninety and sixty more degrees and cyclically switched so that the light passing through the excitation by the sample is enhanced. After receiving the excited light, the light condenser filters the stray light through the receiving light filter disc 234, and then the detection light is detected by the detection probe 237, and the excited light is analyzed through the detection circuit board 238. Whether the sample contains the corresponding substances is judged by judging whether the optical fiber is received or not, and the content of the corresponding substances in the sample is judged by judging the strength of the received optical fiber. The above steps are sequentially carried out by using exciting light with different wavelengths, and a plurality of substances in the sample can be respectively detected.
The bi-directional motor 231 synchronously drives the light source module 232, the transmitting light filter wheel 233 and the receiving light filter wheel 234 to rotate, so that the transmitting light filters and the receiving light filters are in one-to-one correspondence. When the fiber optic collection optic 236 receives the excited light, the receive filter wheel 234 needs to be adjusted individually. After the light source module 232 is adjusted by the bi-directional motor 231, the receiving optical filter aligned with the optical fiber condenser 236 corresponds to the wavelength of the received light. The present invention makes the adjustment of the receive optical filter wheel 234 more convenient and accurate.
Furthermore, a rotary positioning sensor 239 is further disposed beside the emission light filter disk 233, the rotary positioning sensor 239 is electrically connected to a control circuit board, and the control circuit board is electrically connected to the bi-directional motor 231. The rotational positioning sensor 239 can detect the rotational position of the light source plate 2321, thereby determining whether the corresponding excitation light source 2322 is rotated in place, and sending a signal to the control circuit board. When the excitation light source 2322 does not rotate in place, the control circuit board controls the bi-directional motor 231 to operate, so that the excitation light source 2322 rotates in place.
As shown in fig. 16, the temperature-variable amplification module 26 includes a module housing 263, a suspended heating block 261 is installed in the module housing 263, the light-receiving cone 235 is connected to a receiving end of the suspended heating block 261 through an optical fiber, the optical fiber condenser 236 is connected to an emitting end of the suspended heating block 261 through an optical fiber, and the peltier 262 is disposed on both sides of the suspended heating block 261. Heat sinks 264 are mounted on both sides of the module housing 263. The side of the heat sink 264 remote from the peltier 262 is fitted with a heat sink fan 265. The microfluidic PCR chip of the kit is inserted into the suspended heating block 261, and the suspended heating block 261 and the Peltier 262 can be controlled to be stable between ninety-many degrees and sixty-many degrees and can be kept stable and switched circularly, so that light of laser after the exciting light passes through the sample reagent can be exponentially enhanced, and the detection of the excited light is facilitated.
As shown in fig. 9, a slot for inserting the microfluidic PCR chip 2103 is disposed on the pyrolysis module 29, and the pyrolysis module 29 is used for controlling the temperature to heat the chip pyrolysis chamber.
As shown in fig. 13, the frame 21 is provided with a stopper groove 211 for stopping the puncture rack 252. The limiting groove 211 can limit the puncture frame 252, and ensure that the puncture frame 252 can accurately move along a straight line, thereby ensuring that the puncture rod 253 can accurately puncture the sealing film.
The invention is not limited to the above alternative embodiments, and any other various forms of products can be obtained by anyone in the light of the present invention, but any changes in shape or structure thereof, which fall within the scope of the present invention as defined in the claims, fall within the scope of the present invention.
Claims (10)
1. A piston lifting and film sealing puncturing device of a full-automatic PCR analysis system is characterized by comprising a framework (21), a piston lifting module (24) is arranged on the framework (21), the output end of the piston lifting module (24) is connected with a push rod (244) used for driving a rubber piston in a kit (210) to move, and the push rod (244) extends into the kit (210); the automatic reagent box is characterized in that a rotating plate (251) is hinged to the framework (21), one end of the rotating plate (251) is lapped on the output end of the piston lifting module (24), a puncture frame (252) is hinged to the other end of the rotating plate (251), a plurality of puncture rods (253) used for puncturing liquid reagent sealing films in the reagent box (210) are connected to the other end of the puncture frame (252), and the puncture rods (253) extend into the reagent box (210).
2. The piston lifting and film sealing puncturing device of the full-automatic PCR analysis system according to claim 1, wherein a control circuit board (22) is further installed on the framework (21), and the piston lifting module (24) is electrically connected with the control circuit board (22).
3. The piston lifting and film sealing puncturing device of the full-automatic PCR analysis system according to claim 1, wherein the piston lifting module (24) comprises a lifting motor (241) installed on the frame (21), the lifting motor (241) is electrically connected with the control circuit board (22), the output end of the lifting motor (241) is connected with a lifting screw (242), the lifting screw (242) is in threaded connection with a lifting plate (243), the push rod (244) is connected on the lifting plate (243), and one end of the rotating plate (251) is lapped on the lifting plate (243).
4. The piston lifting and membrane sealing puncturing device of the full-automatic PCR analysis system according to claim 3, wherein a positioning plate (245) is fixed on the lifting plate (243), a plurality of positioning sensors (246) are arranged on the moving path of the positioning plate (245), and the positioning sensors (246) are electrically connected with the control circuit board (22).
5. The full-automatic PCR analysis system piston lifting and sealing membrane puncturing device according to claim 4, wherein the positioning sensor (246) comprises a squeeze piston position sensor (2461), a middle position sensor (2462) and a puncturing position sensor (2463); when the positioning piece (245) moves to the position of the extrusion piston position sensor (2461), the push rod (244) moves to the lowest end, when the positioning piece (245) moves to the position of the middle position sensor (2462), the puncture rod (253) and the push rod (244) are both separated from the reagent box (210), and when the positioning piece (245) moves to the position of the puncture position sensor (2463), the puncture rod (253) is at the position of puncturing a liquid reagent sealing membrane.
6. The piston lifting and sealing membrane puncturing device of the full-automatic PCR analysis system according to claim 3, wherein the frame (21) is further provided with a plurality of guide rods, guide cylinders (247) are sleeved on the guide rods, and the guide cylinders (247) are fixed on the lifting plate (243).
7. The piston lifting and sealing membrane puncturing device of the full-automatic PCR analysis system according to claim 6, wherein the number of the combination formed by the guide rods and the guide barrels (247) is two, and the two guide barrels (247) are respectively arranged on two sides of the lifting plate (243).
8. The piston lifting and sealing membrane puncturing device of the full-automatic PCR analysis system according to claim 1, wherein a plurality of return springs (254) for bouncing up the puncture rack (252) are connected to the puncture rack (252).
9. The piston lifting and film sealing puncturing device of the full-automatic PCR analysis system according to claim 1, wherein a first touch pressure sensor (255) is installed on the framework (21), and the first touch pressure sensor (255) is triggered after the puncturing frame (252) is lifted.
10. The piston lifting and film sealing puncturing device of the full-automatic PCR analysis system according to any one of claims 1 to 9, wherein a limiting groove (211) for limiting the puncturing rack (252) is arranged on the framework (21).
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Denomination of invention: A fully automatic PCR analysis system piston lifting and sealing membrane puncture device Effective date of registration: 20231117 Granted publication date: 20230711 Pledgee: China Construction Bank Co.,Ltd. Chengdu Wenjiang Branch Pledgor: CHENGDU WEIKANG BIOTECHNOLOGY CO.,LTD. Registration number: Y2023980065925 |
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