CN109370896A - Cartridge is used in a kind of closed four-way multiple fluorescence PCR detection - Google Patents
Cartridge is used in a kind of closed four-way multiple fluorescence PCR detection Download PDFInfo
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- CN109370896A CN109370896A CN201811391521.9A CN201811391521A CN109370896A CN 109370896 A CN109370896 A CN 109370896A CN 201811391521 A CN201811391521 A CN 201811391521A CN 109370896 A CN109370896 A CN 109370896A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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Abstract
The invention discloses a kind of closed four-way multiple fluorescence PCR detection cartridges, including upper cover, ontology, PCR reaction tube and pedestal, pedestal, which is connect by wedge type card slot with driving mechanism, can complete rotary motion, there are four guide post and spring mechanisms for chassis interior simultaneously, base plate can be made to move up and down along guide post, to drive the U-shaped ejector pin mechanism of chassis interior to move up and down, the connection of reagent trough is realized by the rubber gasket that U-shaped thimble pokes ontology reagent trench bottom；Piston rod can be inserted in ontology intermediate cylindrical hole slot, piston rod top connection driving mechanism can move up and down, the present invention can be by two steps of paramagnetic particle method nucleic acid extraction and fluorescence quantitative PCR detection, it is completed in a small closed box body, the nucleic acid extraction and fluorescent PCR detection that can be used under no molecular diagnostic laboratories environment, have many advantages, such as that compact-sized, easily operated, structure is simple, easily assembly, effectively improves the environment fitness of molecular diagnosis class product.
Description
Technical field
It is extracted the present invention relates to a kind of biotinylated nucleic acid molecule and detection cartridge, specially a kind of closed four-way is multiple
Cartridge is used in fluorescent PCR detection.
Background technique
Nucleic acid extraction and detection are an important process of molecular diagnosis, but in the extraction and detection process of nucleic acid,
It is also easy to produce Aerosol Pollution, result is made false positive occur, conventional molecular diagnosis needs in specific molecular diagnostic laboratories
It carries out, seriously constrains the application and popularization of this technology.
Summary of the invention
The technical problem to be solved by the present invention is to overcome existing routine molecular diagnostics to need in specific molecular diagnosis reality
Indoor progress is tested, the defect of application and the popularization of this technology is seriously constrained, a kind of enclosed cartridge is provided, can be used for
The research and development of POCT class molecular diagnosis instrument product.
In order to solve the above-mentioned technical problems, the present invention provides the following technical solutions:
A kind of closed detection of four-way multiple fluorescence PCR cartridge, including upper cover, ontology, PCR reaction tube and pedestal,
An enclosure space is integrally formed, pedestal, which is connect by wedge type card slot with driving mechanism, can complete rotary motion, while pedestal
Internal there are four guide post and spring mechanisms, base plate can be made to move up and down along guide post, to drive the U of chassis interior
Type ejector pin mechanism moves up and down, and the connection of reagent trough is realized by the rubber gasket that U-shaped thimble pokes ontology reagent trench bottom；
Piston rod can be inserted in ontology intermediate cylindrical hole slot, and piston rod top connection driving mechanism can move up and down, and passes through draught head
Reagent in reagent trough is subjected to liquid relief；Body lower part side is provided with 4 PCR reaction tubes.
In the above-mentioned technical solutions, further, the upper cover is provided with waterproof air ventilation membrane, for balancing in reagent chamber
Air pressure.
10 reagent troughs, 2 magnet insertion grooves, reagent trough bottom are provided in the ontology as a preferred technical solution,
Portion has delivery hole blend rubber gasket to be fully sealed.
Based on the above technical solution, it is preferred that the ontology side is provided with 4 PCR reaction interface tubes, interface
Each PCR reaction tube is provided with 2 holes, lower opening is that hole is venthole on inlet opening.
Preferably, the PCR reaction tube uses quad flat structure, and 2 interfaces are arranged, are all made of pagoda header structure.
Further, the pedestal be divided into three parts can with respect to movable part, outside be base housing fixed with ontology, it is interior
Part is valve cover and U-shaped needle stand two parts, and entire inside can rotate in base housing, and U-shaped needle stand can be with respect to valve
Lid moves up and down.
The chassis interior is provided with 4 spring leads as a preferred technical solution, guarantees that U-shaped needle stand is transported up and down
Dynamic directional precision.
In addition, the chassis interior is provided with U-shaped needle, move up and down with U-shaped needle stand, to pierce through the rubber of reagent trench bottom
Glue gasket realizes intercommunication between reagent trough.
When the U-shaped needle stand rotary motion under the action of the driving mechanism of pedestal, driven above by the connection of 4 guide posts
Valve cover rotate together, U-shaped needle stand jacks up upwards under the action of the driving mechanism after rotateing in place, and makes U-shaped needle-penetration rubber
Gasket, by twitching the piston rod of drawing liquid pump, completes reagent from reagent trough to realize that intermediate drawing liquid pump is communicated with reagent chamber
The middle movement to drawing liquid pump, U-shaped needle stand moves downward under the action of the drive mechanism after the completion, drives U-shaped needle from rubber gasket
Middle extraction, rotates under the action of the driving mechanism again later, makes pin hole to other reagent trough, repeats upward jack-up movement, make U
Type needle-penetration rubber gasket, pushes the piston rod of drawing liquid pump to be pressed into reagent in new reagent trough.To complete to different examinations
Liquid in agent slot and PCR pipe is shifted.
Different detection reagents can be added in reagent trough and PCR pipe, rotary U-type needle stand is connected to different examinations using U-shaped needle
The liquid filling hole of agent slot and PCR pipe, using intermediate drawing liquid pump piston motion draw reagent trough liquid or to reagent tank liquid into
Row pressure-vaccum mix, cooperation magnetic material the combination of magnetic bead and nucleic acid may be implemented, be cleaned multiple times, elute, complete nucleic acid extraction and
Purifying.It can be added to extracting in PCR pipe with nucleic acid after purification later, add reagent and simultaneously cooperate matched fluorescent quantitation
The hot well of PCR detector, can be used for fluorescence quantitative PCR detection.
The beneficial effect of the present invention compared with the existing technology is: closed four-way multiple fluorescence PCR detection of the invention
It can be complete in a small closed box body by two steps of paramagnetic particle method nucleic acid extraction and fluorescence quantitative PCR detection with cartridge
At, can be used for nucleic acid extraction under no molecular diagnostic laboratories environment and fluorescent PCR detection, have it is compact-sized, be easy to grasp
Make, structure is simple, easily assembly the advantages that, effectively improve the environment fitness of molecular diagnosis class product.
Detailed description of the invention
Attached drawing is used to provide further understanding of the present invention, and constitutes part of specification, with reality of the invention
It applies example to be used to explain the present invention together, not be construed as limiting the invention.In the accompanying drawings:
Fig. 1 is structural schematic diagram of the invention；
Fig. 2 is ontology reagent slot structure schematic diagram of the invention；
Fig. 3 is pedestal diagrammatic cross-section of the invention；
Fig. 4 is chassis interior structural schematic diagram of the invention.
Specific embodiment
Hereinafter, preferred embodiments of the present invention will be described with reference to the accompanying drawings, it should be understood that preferred reality described herein
Apply example only for the purpose of illustrating and explaining the present invention and is not intended to limit the present invention.
Embodiment
As shown in Figs 1-4, a kind of closed detection of four-way multiple fluorescence PCR cartridge, including upper cover 1, ontology 2, PCR
Reaction tube 3 and pedestal 4, are integrally formed an enclosure space, and pedestal 4 is connected by the wedge type card slot and driving mechanism of U-shaped needle stand 204
Rotary motion can be completed by connecing, while the inside of pedestal 4 is there are four guide post 202 and spring 203, and U-shaped needle stand 204 can be made along leading
It moves up and down to column 202, so that the U-shaped thimble 206 inside pedestal 4 be driven to move up and down, ontology 2 is poked by U-shaped thimble 206
The rubber gasket of reagent trench bottom realizes the connection of reagent trough；Piston rod, piston can be inserted in the intermediate drawing liquid pump 112 of ontology 2
Bar top connection driving mechanism can move up and down, and the reagent in reagent trough is carried out liquid relief by draught head；2 lower part one of ontology
Side is provided with 4 PCR reaction tubes 3.
Interior place of mixed liquor (200 μ L), reagent trough 109 that cracking and magnetic bead are placed in splitter 106 combines liquid (200 μ
L), sample to be tested and Proteinase K (200 μ L) is added in reagent trough 101, places cleaning solution I (345 μ L) in reagent trough 102, reagent
Cleaning solution II (345 μ L) is placed in slot 104, places cleaning solution III (345 μ L) in reagent trough 107, placed in reagent trough 111 go from
Sub- water (345 μ L) places eluent (60 μ L) in reagent trough 110, and reagent trough 108 does not put reagent and does waste liquid pool use, in PCR pipe
The preset PCR reaction solution (20 μ L) that can detecte different purpose nucleic acid segments respectively.
It needs for cartridge to be placed in inside detector before cartridge work, guarantees 4 PCR pipes to positive insertion hot trap, intermediate cylindrical
Piston is inserted into slot 112, piston rod upper end connects driving mechanism, and the slot of magnet insertion groove 105 is to positive bar magnet stick, magnet
Stick upper end is connected with driving mechanism, and the wedge groove and wedge drive of pedestal 204 are fitted close, and completes the correct of entire cartridge
Installation.
Concrete operations process is as follows:
1, the U-shaped needle stand 204 of rotating base, band movable valve top cover 201 and U-shaped needle 206 rotate, to positive reagent trough 109, the bottom of by
The U-shaped needle stand 204 of seat jacks up upwards, makes the rubber seal of the gasket and reagent trough 109,112 of U-shaped needle-penetration valve top cover 201
Pad, by the piston rod pull in 112, makes the reagent in reagent trough 109 enter reagent trough 112, U-shaped under the action of spring 203
Needle stand 204 resets, and U-shaped needle is extracted, rotary U-type needle stand 204 from gasket, makes U-shaped needle 206 to positive reagent trough 106, again
The U-shaped needle stand 204 of pedestal is jacked up upwards, makes the gasket and splitter 106, reagent trough 112 of U-shaped needle-penetration valve top cover 201
Rubber gasket so that the reagent in reagent trough 112 is entered splitter 106 the piston rod pushing tow in reagent trough 112；It repeats
The above movement, by reagent trough 101 sample and Proteinase K be transferred to splitter 106.Liquid in splitter 106 is carried out
After pressure-vaccum mixes, 10 minutes are stood, while heating is carried out to the reagent in splitter 106 by heating device and is accelerated in sample
Cell cracking, nucleic acid are released in liquid, and magnetic bead is under the action of combining liquid, in conjunction with nucleic acid；
2, magnet strip is dropped to by 105 bottom of magnet insertion groove by driving mechanism, magnetic bead is adsorbed by magnet,
After absorption, the U-shaped needle stand 204 of rotating base, band movable valve top cover 201 and U-shaped needle 206 rotate, to positive splitter 106, by pedestal U
Type needle stand 204 jacks up upwards, makes the gasket of U-shaped needle-penetration valve top cover 201 and the rubber gasket of splitter 106, will try
Piston rod pull in agent slot 112, makes the reagent in splitter 106 enter reagent trough 112, U-shaped needle under the action of spring 203
Seat 204 resets, and U-shaped needle is extracted from gasket, rotary U-type needle stand 204, makes U-shaped needle 206 to positive reagent trough 108, again will
The U-shaped needle stand of pedestal 204 jacks up upwards, makes the gasket and reagent trough 108, reagent trough 112 of U-shaped needle-penetration valve top cover 201
Piston rod pushing tow in reagent trough 112 is made the reagent in reagent trough 112 enter 108 by rubber gasket；
3, it repeats liquid relief movement the cleaning solution I in reagent trough 102 is transferred in splitter 106, removes magnet, use reagent
In slot 112 piston push-and-pull to the tank liquid of splitter 106 carry out inhale blows mixings, to magnetic bead surfaces combine remove it is epinucleic its
He cleans impurity；
4, after cleaning, magnet is placed on to the bottom of splitter 106, magnetic bead is adsorbed, after absorption, it is dynamic to repeat liquid relief
Make, waste liquid is transferred in reagent trough 108；
5, it repeats liquid relief movement the cleaning solution II in reagent trough 104 is transferred in splitter 106, magnet is removed, with 112
Interior piston push-and-pull, which inhale to 106 tank liquid of splitter, blows mixing, and magnetic bead surfaces are combined and remove epinucleic other impurities
Carry out secondary cleaning；
6, after cleaning, magnet is placed on to the bottom of splitter 106, magnetic bead is adsorbed, after absorption, it is dynamic to repeat liquid relief
Make, waste liquid is transferred in reagent trough 108；
7, it repeats liquid relief movement the cleaning solution II in reagent trough 107 is transferred in reagent trough 106, magnet is removed, with examination
In agent slot 112 piston push-and-pull to 106 tank liquid of splitter carry out inhale blows mixings, by magnetic bead surfaces combine remove it is epinucleic its
He is cleaned impurity three times；
8, after cleaning, magnet is placed on to the bottom of splitter 106, magnetic bead is adsorbed, after absorption, it is dynamic to repeat liquid relief
Make, waste liquid is transferred in reagent trough 108；
9, liquid relief movement is repeated, the eluent in reagent trough 110 is added in splitter 106, magnet is removed, uses reagent
Piston push-and-pull in slot 112, which inhale to 106 tank liquid of splitter, blows mixing, the Nucleic Acid Elution that magnetic bead surfaces are combined；
10, after eluting, magnet is placed on to the bottom of splitter 106, magnetic bead is adsorbed, after absorption, filter core suction nozzle
It liquid in splitter 106 is sucked out, is transferred in reagent trough 103, the liquid in reagent trough 103, which contains, to be extracted and after purification
Nucleic acid can be used for fluorescence quantitative PCR detection；
11, liquid relief movement is repeated, the nucleic acid solution of the purifying of 10 μ L is taken respectively from reagent trough 103, is added to each PCR
Liquid in PCR pipe inhale by the piston push-and-pull in reagent trough 112 in pipe and blows mixing, completes the preparation of PCR reaction system；
12, cartridge is placed in the hot well of matched fluorescent quantitative PCR detector, starts to carry out fluorescent quantitation
The detection of PCR.
This set mechanism can allow liquid relief movement in the box body being sealed, can to the liquid in different reagent troughs and PCR pipe into
Row transfer.Cartridge can be used for nucleic acid extraction and fluorescent PCR detection under no molecular diagnostic laboratories environment, have structure tight
It gathers, is easily operated, environment tolerance is high, can detect the advantages that multiple indexs.
Finally, it should be noted that the foregoing is only a preferred embodiment of the present invention, it is not intended to restrict the invention,
Although the present invention is described in detail referring to the foregoing embodiments, for those skilled in the art, still may be used
To modify the technical solutions described in the foregoing embodiments or equivalent replacement of some of the technical features.
All within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in of the invention
Within protection scope.
Claims (8)
1. a kind of closed detection of four-way multiple fluorescence PCR cartridge, including upper cover, ontology, PCR reaction tube and pedestal, whole
Body forms an enclosure space, it is characterised in that: the pedestal is connect with driving mechanism by wedge type card slot and can complete to rotate
Movement, while there are four guide post and spring mechanisms for the chassis interior, and base plate can be made to move up and down along guide post, from
And the U-shaped thimble of chassis interior is driven to move up and down, the rubber gasket of intrinsic reagent trench bottom is poked by U-shaped thimble
Realize the connection of reagent trough；Cylinder hole slot is equipped among the ontology, piston rod can be inserted in cylinder hole slot, and piston rod top connects
The driving mechanism is connect, the reagent in reagent trough is carried out by liquid relief by draught head；The body lower part side is provided with 4
PCR reaction tube.
2. closed four-way multiple fluorescence PCR detection cartridge according to claim 1, which is characterized in that on described
Lid is provided with waterproof air ventilation membrane.
3. closed four-way multiple fluorescence PCR detection cartridge according to claim 1, which is characterized in that described
It is provided with 10 reagent troughs, 2 magnet insertion grooves in vivo, reagent trench bottom has the sealing of delivery hole blend rubber gasket.
4. closed four-way multiple fluorescence PCR detection cartridge according to claim 1, which is characterized in that described
Body side is provided with 4 PCR reaction interface tubes, and interface is that each PCR reaction tube is provided with 2 holes, and lower opening is hole on inlet opening
For venthole.
5. closed four-way multiple fluorescence PCR detection cartridge according to claim 1, which is characterized in that described
PCR reaction tube uses quad flat structure, and 2 interfaces are arranged, are all made of pagoda header structure.
6. closed four-way multiple fluorescence PCR detection cartridge according to claim 1, which is characterized in that the bottom
Seat is divided into three parts can be with respect to movable part, and outside is that base housing is fixed with ontology, and inside is divided into valve cover and U-shaped needle stand two
Part, entire inside can rotate in base housing, and U-shaped needle stand can move up and down with respect to valve cover.
7. closed four-way multiple fluorescence PCR detection cartridge according to claim 1, which is characterized in that the bottom
Seat is internally provided with 4 spring leads.
8. closed four-way multiple fluorescence PCR detection cartridge according to claim 1, which is characterized in that the bottom
Seat be internally provided with U-shaped needle, by U-shaped needle stand move up and down pierce through reagent trench bottom rubber gasket, realize reagent trough it
Between intercommunication.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811391521.9A CN109370896A (en) | 2018-11-21 | 2018-11-21 | Cartridge is used in a kind of closed four-way multiple fluorescence PCR detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811391521.9A CN109370896A (en) | 2018-11-21 | 2018-11-21 | Cartridge is used in a kind of closed four-way multiple fluorescence PCR detection |
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| Publication Number | Publication Date |
|---|---|
| CN109370896A true CN109370896A (en) | 2019-02-22 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201811391521.9A Pending CN109370896A (en) | 2018-11-21 | 2018-11-21 | Cartridge is used in a kind of closed four-way multiple fluorescence PCR detection |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109929735A (en) * | 2019-04-25 | 2019-06-25 | 湖南工业大学 | A kind of plunger type detection of nucleic acids integration cartridge and its detection method |
| CN110075935A (en) * | 2019-04-23 | 2019-08-02 | 清华大学 | The micro-fluidic cartridge of multiple determination and application method |
| CN111378574A (en) * | 2020-03-25 | 2020-07-07 | 苏州新海生物科技股份有限公司 | Nucleic acid extraction and amplification device |
| CN111733066A (en) * | 2019-09-29 | 2020-10-02 | 南京岚煜生物科技有限公司 | Integrated reagent cup for sample treatment and detection and method for detecting sample |
| CN112574871A (en) * | 2020-12-16 | 2021-03-30 | 中国科学院合肥物质科学研究院 | Integrated micro-fluidic nucleic acid detection card box of integrated reagent diverter valve |
| CN112877193A (en) * | 2021-04-28 | 2021-06-01 | 苏州雅睿生物技术有限公司 | POCT consumable and detection method |
| CN113699027A (en) * | 2021-08-30 | 2021-11-26 | 成都微康生物科技有限公司 | Piston lifting and sealing membrane puncturing device of full-automatic PCR analysis system |
| CN114517151A (en) * | 2022-02-16 | 2022-05-20 | 东南大学 | Liquid control component and conversion combination use method thereof |
| CN117070334A (en) * | 2023-10-13 | 2023-11-17 | 鲲鹏基因（北京）科技有限责任公司 | Multi-index detection kit and PCR reaction device |
| CN117535144A (en) * | 2024-01-08 | 2024-02-09 | 杭州杰毅生物技术有限公司 | Rotary type PCR (polymerase chain reaction) extraction integrated cartridge and fluorescence detection device thereof |
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2018
- 2018-11-21 CN CN201811391521.9A patent/CN109370896A/en active Pending
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110075935A (en) * | 2019-04-23 | 2019-08-02 | 清华大学 | The micro-fluidic cartridge of multiple determination and application method |
| WO2020216058A1 (en) * | 2019-04-23 | 2020-10-29 | 清华大学 | Multi-index test microfluidic kit and application method therefor |
| CN109929735A (en) * | 2019-04-25 | 2019-06-25 | 湖南工业大学 | A kind of plunger type detection of nucleic acids integration cartridge and its detection method |
| CN111733066A (en) * | 2019-09-29 | 2020-10-02 | 南京岚煜生物科技有限公司 | Integrated reagent cup for sample treatment and detection and method for detecting sample |
| CN111378574B (en) * | 2020-03-25 | 2023-10-27 | 苏州新海生物科技股份有限公司 | Nucleic acid extraction and amplification device |
| CN111378574A (en) * | 2020-03-25 | 2020-07-07 | 苏州新海生物科技股份有限公司 | Nucleic acid extraction and amplification device |
| CN112574871A (en) * | 2020-12-16 | 2021-03-30 | 中国科学院合肥物质科学研究院 | Integrated micro-fluidic nucleic acid detection card box of integrated reagent diverter valve |
| CN112877193A (en) * | 2021-04-28 | 2021-06-01 | 苏州雅睿生物技术有限公司 | POCT consumable and detection method |
| CN113699027B (en) * | 2021-08-30 | 2023-07-11 | 成都微康生物科技有限公司 | Piston lifting and sealing film puncturing device of full-automatic PCR analysis system |
| CN113699027A (en) * | 2021-08-30 | 2021-11-26 | 成都微康生物科技有限公司 | Piston lifting and sealing membrane puncturing device of full-automatic PCR analysis system |
| CN114517151A (en) * | 2022-02-16 | 2022-05-20 | 东南大学 | Liquid control component and conversion combination use method thereof |
| CN117070334A (en) * | 2023-10-13 | 2023-11-17 | 鲲鹏基因（北京）科技有限责任公司 | Multi-index detection kit and PCR reaction device |
| CN117070334B (en) * | 2023-10-13 | 2024-01-26 | 鲲鹏基因（北京）科技有限责任公司 | Multi-index detection kit and PCR reaction device |
| CN117535144A (en) * | 2024-01-08 | 2024-02-09 | 杭州杰毅生物技术有限公司 | Rotary type PCR (polymerase chain reaction) extraction integrated cartridge and fluorescence detection device thereof |
| CN117535144B (en) * | 2024-01-08 | 2024-05-07 | 杭州杰毅生物技术有限公司 | Rotary type PCR (polymerase chain reaction) extraction integrated cartridge and fluorescence detection device thereof |
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