CN117535144A - Rotary type PCR (polymerase chain reaction) extraction integrated cartridge and fluorescence detection device thereof - Google Patents
Rotary type PCR (polymerase chain reaction) extraction integrated cartridge and fluorescence detection device thereof Download PDFInfo
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- CN117535144A CN117535144A CN202410020319.4A CN202410020319A CN117535144A CN 117535144 A CN117535144 A CN 117535144A CN 202410020319 A CN202410020319 A CN 202410020319A CN 117535144 A CN117535144 A CN 117535144A
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- 238000001917 fluorescence detection Methods 0.000 title claims abstract description 125
- 238000000605 extraction Methods 0.000 title claims abstract description 117
- 238000003752 polymerase chain reaction Methods 0.000 title description 23
- 239000012295 chemical reaction liquid Substances 0.000 claims abstract description 7
- 239000007788 liquid Substances 0.000 claims description 29
- 238000001514 detection method Methods 0.000 claims description 25
- 230000000670 limiting effect Effects 0.000 claims description 20
- 238000007789 sealing Methods 0.000 claims description 14
- 239000007795 chemical reaction product Substances 0.000 claims description 7
- 238000009434 installation Methods 0.000 claims description 4
- 230000002829 reductive effect Effects 0.000 abstract description 6
- 150000007523 nucleic acids Chemical class 0.000 description 5
- 102000039446 nucleic acids Human genes 0.000 description 5
- 108020004707 nucleic acids Proteins 0.000 description 5
- 239000004065 semiconductor Substances 0.000 description 5
- 238000004140 cleaning Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 3
- 229910052782 aluminium Inorganic materials 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000012123 point-of-care testing Methods 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000003480 eluent Substances 0.000 description 2
- 239000006166 lysate Substances 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1894—Cooling means; Cryo cooling
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The utility model provides an integrative cartridge of rotation type extraction PCR and fluorescence detection device thereof, is including extracting cartridge, fluorescence detection cartridge, and fluorescence detection cartridge rotatable installs on extracting the cartridge, is equipped with a plurality of extraction cavity and application of sample through-hole on extracting the cartridge, is equipped with a plurality of fluorescence detection cavity in the fluorescence detection cartridge, and the reaction liquid in the extraction cavity removes to in the fluorescence detection cavity through the application of sample through-hole. Through connect extraction card box, fluorescence detection card box with the structure of stacking for the required card box structure of fluorescence detection occupies less area, has simplified the requirement in laboratory greatly, has reduced simultaneously the requirement to outside pipetting module motion mechanism.
Description
Technical Field
The invention belongs to the technical field of sample detection devices, and particularly relates to a rotary extraction and PCR integrated cartridge and a fluorescence detection device thereof.
Background
Nucleic acid is a basic unit representing genetic characteristics of a living body, and nucleic acid detection is advanced biological detection at a molecular level, and point-of-care detection (Point of Care Testing, POCT) generally refers to a detection mode which is performed on a sampling site and rapidly obtains a detection result by using a portable analytical instrument and a matched reagent. The existing molecular diagnosis POCT instrument comprises the following two fluorescence detection modes, (1) the steps of extraction and PCR fluorescence quantification are usually respectively finished in different cassettes, and translation and up-and-down movement are required to be carried out through an external pipetting mechanism, so that the complexity of a fluorescence detection device is increased, the reliability of the instrument is reduced, the miniaturization of the instrument is not facilitated, and the cost of the instrument is increased; (2) The cartridge for realizing the full-closed environment from nucleic acid extraction to fluorescence detection by utilizing the micro-fluidic technology, but the micro-fluidic technology realizes the simplification and miniaturization of the instrument structure, but has higher cost, higher difficulty of the micro-fluidic technology, inferior experimental stability to the traditional experimental mode and unfavorable popularization.
Disclosure of Invention
Aiming at the defects, the technical problem to be solved by the invention is to provide a rotary extraction PCR integrated cartridge and a fluorescence detection device thereof, which are used for simplifying the structure of the traditional fluorescence detection device and improving the use stability of the fluorescence detection device.
In order to solve the technical problems, the invention adopts the technical proposal that,
a rotary type PCR extraction integrated cartridge comprises
The extraction cartridge is provided with a plurality of extraction chambers and sample adding through holes;
the fluorescence detection card box is rotatably arranged on the extraction card box, a plurality of fluorescence detection chambers are arranged in the fluorescence detection card box, and reaction liquid in the extraction chamber moves into the fluorescence detection chamber through the sample adding through hole.
As a preferable scheme of the invention, the fluorescence detection cartridge is provided with a heat conduction sealing member which is abutted with the extraction cartridge, the heat conduction sealing member is provided with a sample adding hole corresponding to the fluorescence detection chamber, and the reaction liquid moves into the fluorescence detection chamber through the sample adding hole.
As a preferable scheme of the invention, the bottom of the extraction cartridge is provided with a bottom end plate, the bottom end plate is provided with a holding plate, the fluorescence detection cartridge is rotatably arranged on the bottom end plate through the holding plate, and the bottom end plate is provided with a bottom through hole opposite to the sample adding through hole.
A fluorescence detection device comprises
The rotary extraction PCR integrated cartridge;
the cartridge seat is internally provided with an installation cavity for installing a rotary type extraction and PCR integrated cartridge, the cartridge seat is provided with a rotation limiting piece, and the rotation limiting piece is connected with the extraction cartridge and/or the fluorescence detection cartridge so as to enable the extraction cartridge and the fluorescence detection cartridge to simultaneously rotate or relatively rotate;
and the driving piece is connected with the rotary extraction and PCR integrated cartridge and drives the extraction cartridge and/or the fluorescence detection cartridge to rotate.
As a preferable scheme of the invention, the card box seat is rotatably connected with the driving piece, the extraction card box is connected with the card box seat, the fluorescence detection card box is connected with the driving piece, and the driving piece drives the fluorescence detection card box to rotate, and the fluorescence detection card box drives the extraction card box to rotate.
As a preferable mode of the invention, the rotation limiting member is arranged on the outer side of the cartridge seat so as to limit the rotation of the cartridge seat or the extraction cartridge through the rotation limiting member.
As a preferable scheme of the invention, the driving piece comprises a driving shaft and a heat conduction seat arranged on the driving shaft, a heat conduction slot is arranged in the heat conduction seat, and the fluorescence detection card box is inserted in the heat conduction slot.
As a preferable scheme of the invention, one side of the heat conduction slot is provided with a detection through hole, and the fluorescence detection device further comprises a fluorescence detector which carries out fluorescence detection on the liquid in the fluorescence detection cavity through the detection through hole.
As a preferable scheme of the invention, the fluorescence detection device further comprises a magnetic attraction mechanism, wherein the magnetic attraction mechanism comprises a magnetic suction head and a rotary driving piece connected with the magnetic suction head, and the magnetic suction head is driven to rotate through the rotary driving piece so as to magnetically attract the rotary extraction PCR integrated cartridge through the magnetic suction head.
As a preferable scheme of the invention, the side walls of the cartridge seat and the rotary extraction PCR integrated cartridge are provided with openings, the magnetic suction heads are matched with the openings, and the magnetic suction heads magnetically suck the reaction liquid in the extraction cavity through the openings.
The invention has the beneficial effects that (1) the extraction cartridge and the fluorescence detection cartridge are connected in a stacked structure, so that the cartridge structure required by fluorescence detection occupies a smaller area, the requirements of a laboratory are greatly simplified, the requirements on the movement mechanism of an external pipetting module are reduced, and the equipment stability of the fluorescence detection device is improved.
(2) The extraction card box and the fluorescence detection card box are switched in two modes of simultaneous rotation and relative rotation through the rotation limiting piece, so that the operation of adding liquid into the reaction chamber of the extraction card box and adding liquid into each fluorescence detection chamber is conveniently realized, the complexity of the mechanical structure of the whole instrument is reduced, the instrument can be made to be smaller in size, and the cost is reduced.
Drawings
FIG. 1 is a schematic diagram of the structure of a rotary extraction PCR cartridge.
FIG. 2 is a cross-sectional view of a rotary extraction PCR cartridge.
Fig. 3 is a schematic structural view of the extraction cartridge.
Fig. 4 is a schematic structural view of the fluorescence detection device.
FIG. 5 is a schematic diagram of the structure of the rotary extraction PCR cartridge of FIG. 4.
Fig. 6 is a schematic view of the structure of fig. 5 after the housing of the rack is removed.
Fig. 7 is a perspective cross-sectional view of fig. 6.
Fig. 8 is a plan sectional view of fig. 6.
Reference numerals: the device comprises an extraction cartridge 1, an extraction chamber 1-1, a reaction chamber 1-1-1, a waste liquid storage chamber 1-1-2, a pretreatment liquid storage chamber 1-1-3, a lysate storage chamber 1-1-4, a first cleaning liquid storage chamber 1-1-5, a second cleaning liquid storage chamber 1-1-6, an eluent storage chamber 1-1-7, a reserved reaction chamber/storage chamber 1-1-8, a nucleic acid storage chamber 1-1-9, a sample adding through hole 1-2, a bottom end plate 1-3, a holding plate 1-4, a bottom through hole 1-5, a fluorescence detection cartridge 2, a fluorescence detection chamber 2-1, a heat conducting sealing member 3, a cartridge seat 4, an installation cavity 4-1, a limit groove 4-2, a detection through groove 4-3, a rotation limit member 5, a driving member 6, a driving shaft 6-1, a heat conducting seat 6-2, a heat conducting slot 6-3, a heat radiating fin 6-4, a fan 6-5, a 6-6, a fluorescence detector 7, a magnetic attraction mechanism 8-1, a rotation driving member 8-2, a heat conducting fin 6-6, a fluorescence detector 7, a magnetic attraction mechanism 8-2, a rotation driving pulley 8-2, a rotation pulley 9, a heat conducting fin 10, a rotation sealing plate 11, a frame seat 14, a rotation shaft seat 12, a rotation plate 11, a rotation seat 12, a rotation seat 11, a rotation plate, a sealing plate, a seat 11, a rotation seat 11 and a seat 13.
Detailed Description
The invention is further described below with reference to the accompanying drawings.
The utility model provides a integrative cartridge of rotation type extraction PCR, includes extraction cartridge 1, fluorescence detection cartridge 2, and fluorescence detection cartridge 2 rotatable installs on extraction cartridge 1, is equipped with a plurality of extraction cavity 1-1 and application of sample through-hole 1-2 on extraction cartridge 1, is equipped with a plurality of fluorescence detection cavity 2-1 in the fluorescence detection cartridge 2, and the reaction product in the extraction cavity 1-1 removes to in the fluorescence detection cavity 2-1 through application of sample through-hole 1-2.
A plurality of reagent tubes facing the fluorescence detection cassette 2 are arranged in the extraction cassette 1, an aluminum film is arranged at the top of the extraction cassette 1, so that a plurality of closed extraction chambers 1-1 are formed in the extraction cassette 1, in this embodiment, 10 extraction chambers 1-1 are arranged in the extraction cassette 1, 10 extraction chambers 1-1 are respectively reaction chambers 1-1-1, waste liquid storage chambers 1-1-2, pretreatment liquid storage chambers 1-1-3, lysate storage chambers 1-1-4, first cleaning liquid storage chambers 1-1-5, second cleaning liquid storage chambers 1-1-6, eluent storage chambers 1-1-7, 2 reaction chambers/storage chambers 1-1-8 for reservation, and nucleic acid storage chambers 1-1-9 required by an extraction process, and a sample or specimen to be detected is moved into the reaction chambers from the liquid in each chamber through an external liquid dispenser (liquid dispenser) according to experimental requirements, so as to form reaction products in the reaction chambers; the sample adding through holes 1-2 are arranged on one side of the reaction chamber, so that the reaction liquid in the reaction chamber can be conveniently moved into the fluorescence detection card box 2 through the sample adding through holes 1-2.
The fluorescent detection card box 2 is internally provided with a plurality of fluorescent detection chambers 2-1, the top of the fluorescent detection card box 2 is also provided with an aluminum film, and when an external liquid shifter is used for adding samples to the fluorescent detection chambers 2-1, the liquid shifter can puncture the aluminum film so as to add reaction products into the fluorescent detection chambers 2-1, and the fluorescent detection chambers 2-1 are opposite to the sample adding through holes 1-2.
The extraction card box 1 and the fluorescence detection card box 2 are cylindrical, in the working process, the liquid transfer device only moves up and down, liquid in different cavities is transferred by the liquid transfer device through rotating the extraction card box 1 and the fluorescence detection card box 2, and the whole volume of the fluorescence detection device can be reduced.
The bottom of the extraction cartridge 1 is provided with a bottom end plate 1-3, the bottom end plate 1-3 is provided with a holding plate 1-4, the fluorescence detection cartridge 2 is rotatably arranged on the bottom end plate 1-3 through the holding plate 1-4, and the bottom end plate 1-3 is provided with a bottom through hole 1-5 opposite to the sample adding through hole 1-2.
The extraction cartridge 1 and the fluorescence detection cartridge 2 rotate relatively, so that the pipettor can conveniently add the extraction reaction products to each fluorescence detection chamber 2-1 through the sample adding through holes 1-2.
The fluorescence detection cartridge 2 is provided with a heat conduction sealing member 3 which is abutted with the extraction cartridge 1, the heat conduction sealing member is provided with a sample adding hole corresponding to the fluorescence detection chamber 2-1, and the reaction product moves into the fluorescence detection chamber 2-1 through the sample adding hole.
The heat conduction sealing piece 3 comprises a heat conduction sheet 10 and a sealing sheet 11, the heat conduction sheet 10 is used for being in contact with a heat source to heat the top of the fluorescence detection chamber 2-1, detection liquid is prevented from condensing at the top of the fluorescence detection chamber 2-1 in the fluorescence detection process, the sealing sheet 11 is a rubber piece, and when the extraction cartridge 1 and the fluorescence detection cartridge 2 are combined, the sealing sheet 11 is in an extrusion state, on one hand, the liquid which is partially vaporized in the detection process can be prevented from streaming among the fluorescence detection chambers 2-1, on the other hand, friction force between the extraction cartridge 1 and the fluorescence detection cartridge 2 can be increased, and the rotation of the extraction cartridge 1 is facilitated in the rotation process of the fluorescence detection cartridge 2.
The fluorescence detection device comprises the rotary extraction PCR integrated cartridge, a cartridge seat 4, a driving piece 6 and a rack seat plate 12, wherein the cartridge seat 4 and the driving piece 6 are respectively arranged on the rack seat plate 12, a mounting cavity 4-1 for mounting the rotary extraction PCR integrated cartridge is arranged in the cartridge seat 4, a rotation limiting piece 5 is connected with the extraction cartridge 1 and/or the fluorescence detection cartridge 2, the rotation degree of freedom of the extraction cartridge 1 or the fluorescence detection cartridge 2 towards one side is limited by the rotation limiting piece 5, namely, the extraction cartridge 1 or the fluorescence detection cartridge 2 can only rotate along one direction through the arrangement of the rotation limiting piece 5, when the rotation limiting piece 5 limits the rotation of the extraction cartridge 1 or the fluorescence detection cartridge 2, one of the extraction cartridge 1 or the fluorescence detection cartridge 2 is in a static state, and the other one of the extraction cartridge 1 and the fluorescence detection cartridge 2 is in a rotating state, so that the relative rotation of the extraction cartridge 1 and the fluorescence detection cartridge 2 is realized, and when the rotation limiting piece 5 does not limit the rotation of the extraction cartridge 1 or the fluorescence detection cartridge 2, the extraction cartridge 1 and the fluorescence detection cartridge 2 are simultaneously rotated; the driving part 6 is connected with the rotary extraction and PCR integrated cartridge and drives the extraction cartridge 1 and/or the fluorescence detection cartridge 2 to rotate.
The rotation limiting piece 5 is arranged on the outer side of the cartridge seat 4, so that the rotation of the cartridge seat 4 and the extraction cartridge 1 is limited by the rotation limiting piece 5. In this embodiment, the upper end of the frame seat board 12 is provided with a rotary mounting seat 13, the cartridge seat 4 is rotatably mounted on the rotary mounting seat 13 through a rotation limiting member 5, the rotation limiting member 5 is a one-way bearing, the lower end of the frame seat board 12 is provided with a shoulder sleeve 14, a bearing is arranged in the shoulder sleeve 14, the driving member 6 is rotatably mounted on the frame seat board 12 through the bearing, the cartridge seat 4 and the driving member 6 are rotatably connected, that is, the cartridge seat 4 and the driving member 6 can rotate relatively, the extraction cartridge 1 is connected with the cartridge seat 4, the fluorescence detection cartridge 2 is connected with the driving member 6, the driving member 6 drives the fluorescence detection cartridge 2 to rotate, and the fluorescence detection cartridge 2 drives the extraction cartridge 1 to rotate.
The installation cavity 4-1 is internally provided with a limit groove 4-2, and the outer side of the extraction cartridge 1 is provided with a limit strip which is inserted into the limit groove 4-2, so that the extraction cartridge 1 and the cartridge seat 4 rotate simultaneously.
The driving piece 6 comprises a driving shaft 6-1 and a heat conduction seat 6-2 arranged on the driving shaft 6-1, a heat conduction slot 6-3 is arranged in the heat conduction seat 6-2, and the fluorescence detection card box 2 is inserted into the heat conduction slot 6-3, so that the fluorescence detection card box 2 rotates along with the rotation of the driving shaft 6-1.
A semiconductor radiating fin 6-3 is arranged between the driving shaft 6-1 and the heat conducting seat 6-2, and the semiconductor radiating fin 6-3 heats or cools the heat conducting seat 6-2 by controlling the flow direction of current flowing through the semiconductor radiating fin; to facilitate routing of the wires of the semiconductor heat sink 6-3, the drive shaft 6-1 is a hollow shaft, and the cable extends out of the drive shaft 6-1 through the through hole of the drive shaft 6-1.
The outside of the driving shaft 6-1 is provided with the radiating fins 6-4, the driving shaft 6-1 is radiated through the radiating fins 6-4, the corresponding frame seat board 12 is also provided with the fan 6-5, and the fan 6-5 is arranged opposite to the radiating fins 6-4, so that the radiating efficiency of the driving shaft 6-1 is conveniently improved.
The driving shaft 6-1 is provided with a belt pulley 6-6, and the motor drives the belt pulley 6-6 to rotate through a belt, so that the driving shaft 6-1 is driven to rotate.
The fluorescence detector 7 is arranged on the stand seat board 12, the detection through groove 4-3 is arranged on the cartridge seat 4, the detection through hole 6-4 is arranged on one side of the heat conduction slot 6-3, and when fluorescence detection is carried out, the fluorescence detector 7, the detection through groove 4-3 and the detection through hole 6-4 are positioned on the same straight line, so that the fluorescence detector 7 can conveniently carry out fluorescence detection on liquid in the fluorescence detection cavity 2-1 through the detection through groove 4-3 and the detection through hole 6-4.
The magnetic attraction mechanism 8 is arranged on the frame seat plate 12, the magnetic attraction mechanism 8 comprises a magnetic attraction head 8-1 and a rotary driving piece 8-2 connected with the magnetic attraction head 8-1, and the magnetic attraction head 8-1 is driven to rotate through the rotary driving piece 8-2 so as to magnetically attract the rotary extraction PCR integrated cartridge through the magnetic attraction head 8-1.
The rotary driving piece 8-2 is a direct current motor, the rotary driving piece 8-2 drives the magnetic suction head 8-1 to swing in the range of 90, when the reaction chamber needs to be magnetically sucked, the rotary driving piece 8-2 drives the magnetic suction head 8-1 to stretch into the extraction cartridge 1 to magnetically suck the reaction chamber on the extraction cartridge 1, and when the cartridge seat 4 needs to be rotated, the rotary driving piece 8-2 drives the magnetic suction head 8-1 to rotate to the outer side of the cartridge seat 4, so that the magnetic suction head 8-1 is prevented from interfering with the rotation of the cartridge seat 4.
The cartridge seat 4 and the side wall of the extraction cartridge 1 are provided with openings 9, and the openings 9 are arranged on one side of the reaction chamber, so that the magnetic suction head 8-1 can magnetically suck the reaction liquid in the reaction chamber through the openings 9.
The working principle of the fluorescence detection device is as follows:
(1) Sample adding and mixing reaction steps are carried out on the reaction chamber:
s1.1, a driving shaft 6-1 drives a heat conduction seat 6-2 to rotate, and an extraction cartridge 1 and a fluorescence detection cartridge 2 simultaneously rotate along with the rotation of the driving shaft 6-1;
s1.2, the extraction cartridge 1 rotates, so that an external pipettor is opposite to each extraction chamber 1-1 of the extraction cartridge 1, and liquid in the extraction chamber 1-1 is moved into a reaction chamber;
s1.3, after sample addition is completed, the rotary driving piece 8-2 drives the magnetic suction head 8-1 to extend into the cartridge seat 4, and the reaction chamber on the cartridge seat 4 is magnetically attracted.
(2) Fluorescence detection step:
s2.1, the pipettor is opposite to the reaction chamber of the extraction cartridge 1, and absorbs liquid in the reaction chamber;
s2.2, driving the extraction cartridge 1 to rotate, so that the liquid dispenser faces the sample adding through hole 1-2, and moving the liquid into the fluorescence detection chamber 2-1 by descending the liquid dispenser;
s2.3, controlling the temperature of the heat conduction seat by controlling the semiconductor refrigerating sheet to realize PCR reaction of the fluorescence detection chamber;
s2.4, the fluorescence detector 7 carries out fluorescence detection on the liquid subjected to the PCR reaction in the fluorescence detection chamber 2-1.
In this embodiment, when the extraction cartridge 1 and the fluorescence detection cartridge 2 are simultaneously rotated, the driving shaft 6-1 is rotated in the forward direction, and when the extraction cartridge 1 and the fluorescence detection cartridge 2 are rotated relative to each other, the driving shaft 6-1 is rotated in the reverse direction; in step S1.1, the drive shaft 6-1 is rotated in the forward direction; in step S2.2, the drive shaft 6-1 is rotated in the reverse direction. When the driving shaft 6-1 rotates reversely, the cartridge seat 4 is limited to rotate reversely under the action of the rotation limiting piece 5 (one-way bearing), and meanwhile, the extraction cartridge 1 and the cartridge seat 4 are fixedly connected, so that the extraction cartridge 1 cannot rotate reversely, the fluorescence detection cartridge 2 is directly connected with the driving shaft 6-1, and the relative rotation of the extraction cartridge 1 and the fluorescence detection cartridge 2 is realized; when the driving shaft 6-1 rotates in the forward direction, the rotation limiting piece 5 does not limit the rotation of the cartridge seat 4, and the heat conduction sealing piece 3 between the extraction cartridge 1 and the fluorescence detection cartridge 2 enables the extraction cartridge 1 to rotate along with the rotation of the fluorescence detection cartridge 2 under the action of friction force.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention; thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Although the terms corresponding to the reference numerals in the drawings are used more herein, the possibility of using other terms is not excluded; these terms are used merely for convenience in describing and explaining the nature of the invention; they are to be interpreted as any additional limitation that is not inconsistent with the spirit of the present invention.
Claims (10)
1. A rotary PCR integrated cartridge comprises
An extraction cartridge (1) provided with a plurality of extraction chambers (1-1) and sample-feeding through holes (1-2);
the fluorescence detection card box (2) is rotatably arranged on the extraction card box (1), a plurality of fluorescence detection chambers (2-1) are arranged in the fluorescence detection card box (2), and reaction products in the extraction chambers (1-1) move into the fluorescence detection chambers (2-1) through the sample adding through holes (1-2).
2. The rotary extraction and PCR integrated cartridge as claimed in claim 1, wherein the fluorescence detection cartridge (2) is provided with a heat conducting sealing member (3) abutted with the extraction cartridge (1), the heat conducting sealing member is provided with a sample adding hole corresponding to the fluorescence detection chamber (2-1), and the reaction product moves into the fluorescence detection chamber (2-1) through the sample adding hole.
3. The rotary extraction and PCR integrated cartridge as claimed in claim 1, wherein a bottom end plate (1-3) is arranged at the bottom of the extraction cartridge (1), a holding plate (1-4) is arranged on the bottom end plate (1-3), the fluorescence detection cartridge (2) is rotatably arranged on the bottom end plate (1-3) through the holding plate (1-4), and a bottom through hole (1-5) opposite to the sample adding through hole (1-2) is arranged on the bottom end plate (1-3).
4. A fluorescence detection device is characterized by comprising
A rotary extraction PCR integrated cartridge as claimed in any one of claims 1 to 3;
the device comprises a cartridge seat (4), wherein an installation cavity (4-1) for installing a rotary extraction PCR integrated cartridge is formed in the cartridge seat, a rotation limiting piece (5) is arranged on the cartridge seat (4), and the rotation limiting piece (5) is connected with the extraction cartridge (1) and/or the fluorescence detection cartridge (2) so that the extraction cartridge (1) and the fluorescence detection cartridge (2) rotate simultaneously or relatively;
and the driving piece (6) is connected with the rotary extraction and PCR integrated cartridge and drives the extraction cartridge (1) and/or the fluorescence detection cartridge (2) to rotate.
5. The fluorescence detection device according to claim 4, wherein the cartridge holder (4) is rotatably connected with the driving member (6), the extraction cartridge (1) is connected with the cartridge holder (4), the fluorescence detection cartridge (2) is connected with the driving member (6), the driving member (6) drives the fluorescence detection cartridge (2) to rotate, and the fluorescence detection cartridge (2) drives the extraction cartridge (1) to rotate.
6. A fluorescence detection device according to claim 5, wherein the rotation limiting member (5) is arranged outside the cartridge holder (4) to limit rotation of the cartridge holder (4) or the extraction cartridge (1) by the rotation limiting member (5).
7. The fluorescence detection device according to claim 4, wherein the driving member (6) comprises a driving shaft (6-1) and a heat conducting seat (6-2) arranged on the driving shaft (6-1), a heat conducting slot (6-3) is arranged in the heat conducting seat (6-2), and the fluorescence detection card box (2) is inserted into the heat conducting slot (6-3).
8. A fluorescence detection device according to claim 7, wherein a detection through hole (6-4) is provided on one side of the heat conducting slot (6-3), the fluorescence detection device further comprises a fluorescence detector (7), and the fluorescence detector (7) performs fluorescence detection on the liquid in the fluorescence detection chamber (2-1) through the detection through hole (6-4).
9. The fluorescence detection device according to claim 4, further comprising a magnetic attraction mechanism (8), wherein the magnetic attraction mechanism (8) comprises a magnetic attraction head (8-1) and a rotary driving member (8-2) connected with the magnetic attraction head (8-1), and the magnetic attraction head (8-1) is driven to rotate by the rotary driving member (8-2) so as to magnetically attract the rotary extraction PCR integrated cartridge by the magnetic attraction head (8-1).
10. The fluorescence detection device according to claim 9, wherein the cartridge seat (4) and the side wall of the rotary extraction PCR integrated cartridge are provided with openings (9), the magnetic suction head (8-1) is matched with the openings (9), and the magnetic suction head (8-1) magnetically sucks the reaction liquid in the extraction chamber (1-1) through the openings (9).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410020319.4A CN117535144B (en) | 2024-01-08 | 2024-01-08 | Rotary type PCR (polymerase chain reaction) extraction integrated cartridge and fluorescence detection device thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410020319.4A CN117535144B (en) | 2024-01-08 | 2024-01-08 | Rotary type PCR (polymerase chain reaction) extraction integrated cartridge and fluorescence detection device thereof |
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| CN117535144A true CN117535144A (en) | 2024-02-09 |
| CN117535144B CN117535144B (en) | 2024-05-07 |
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|---|---|---|---|---|
| CN207596860U (en) * | 2017-10-28 | 2018-07-10 | 深圳职业技术学院 | A kind of nucleic acid integration Multiple detection box body |
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| CN217948123U (en) * | 2022-08-05 | 2022-12-02 | 杭州杰毅生物技术有限公司 | Closed card box |
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2024
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| CN110161003A (en) * | 2019-05-17 | 2019-08-23 | 深圳市刚竹医疗科技有限公司 | Optical detection apparatus and real time fluorescent quantitative nucleic acid amplification detection system |
| US20220333180A1 (en) * | 2019-09-10 | 2022-10-20 | Jiaxing Accunome Biotechnology Co., Ltd. | Instrument and method for extracting and detecting nucleic acids |
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| CN212833785U (en) * | 2020-07-06 | 2021-03-30 | 杭州杰毅生物技术有限公司 | Fluorescent detection card box |
| CN114763513A (en) * | 2021-01-29 | 2022-07-19 | 广东润鹏生物技术有限公司 | Molecular diagnostic platform |
| CN215627973U (en) * | 2021-08-06 | 2022-01-25 | 上海思路迪生物医学科技有限公司 | Integrated fluorescence detection system |
| CN217948123U (en) * | 2022-08-05 | 2022-12-02 | 杭州杰毅生物技术有限公司 | Closed card box |
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| CN117535144B (en) | 2024-05-07 |
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