CN215856132U - Full-automatic PCR analytic system light signal amplification detection device - Google Patents

Abstract

The utility model belongs to the technical field of in-vitro diagnosis, and particularly relates to an optical signal amplification detection device of a full-automatic PCR analysis system. The device comprises an optical signal emission detection module and a variable temperature amplification module, wherein the optical signal emission detection module comprises a bidirectional motor, one output shaft of the bidirectional motor is connected with a light source component and an emission light filter disc, and the other output shaft of the bidirectional motor is connected with a receiving light filter disc; the device comprises a transmission light filter disc, a temperature-variable amplification module, a light receiving light cone, a light collecting lens, a detection probe and a detection circuit board, wherein the light receiving light cone is arranged beside the transmission light filter disc and is connected with a receiving end of the temperature-variable amplification module through optical fibers, the optical fiber collecting lens is arranged beside the reception light filter disc and is connected with a transmitting end of the temperature-variable amplification module through optical fibers, one side, away from the optical fiber collecting lens, of the reception light filter disc is provided with the detection probe, and the detection probe is connected with the detection circuit board. The utility model provides an optical signal amplification detection device of a full-automatic PCR analysis system.

Description

Full-automatic PCR analytic system light signal amplification detection device

Technical Field

The utility model belongs to the technical field of in-vitro diagnosis, and particularly relates to an optical signal amplification detection device of a full-automatic PCR analysis system.

Background

The technology of separating and purifying nucleic acid is a basic technology of biochemistry and molecular biology. With the wide application of molecular biology technology in biology, medicine and related fields, the nucleic acid separation and purification technology has been further developed. The development of molecular biology is greatly facilitated by the continuous emergence of various new methods, well-established classical methods and commercial reagent methods. Nucleic acids are biomacromolecules synthesized by the polymerization of many nucleotides, and are one of the most basic substances of life. Nucleic acid is widely present in all animal and plant cells and microorganisms, and nucleic acid in organisms is often combined with protein to form nucleoprotein. With the rapid development of molecular biology in recent years, nucleic acid-based molecular diagnostic and detection techniques have increasingly highlighted important roles in a variety of fields. The existing nucleic acid purification has the advantages of short time consumption, high purification precision and automatic treatment of a large batch of experimental samples; the fluorescence PCR analysis system is a method for monitoring the reaction process of PCR in real time by adding a fluorescent group on the basis of PCR and the change of a fluorescence signal and finally carrying out quantitative analysis on an unknown template. Currently, real-time fluorescence quantitative PCR has become an authoritative method for comparing quantitative differences in gene expression levels among different samples.

The optical signal emission detection module emits excitation light, the excitation light irradiates the fluorescent substance on the sample to excite light with another corresponding wavelength, and the excited light is transmitted to the detection module through the optical fiber. The detection module judges the amount of the corresponding substance contained in the sample through the intensity of the excited light. The temperature-variable amplification module is used for enhancing the intensity of the excited light, so that the detection device can receive the excited light conveniently. However, when the excited light is received, the corresponding receiving optical filter needs to be readjusted, which is troublesome to operate.

SUMMERY OF THE UTILITY MODEL

In order to solve the above problems of the prior art, the present invention is directed to an optical signal amplification detection device for a fully automatic PCR analysis system.

The technical scheme adopted by the utility model is as follows:

an optical signal amplification detection device of a full-automatic PCR analysis system comprises an optical signal emission detection module and a variable temperature amplification module, wherein the optical signal emission detection module comprises a bidirectional motor, one output shaft of the bidirectional motor is connected with a light source component and a transmitting light filter disc, and the other output shaft of the bidirectional motor is connected with a receiving light filter disc; the device comprises a transmission light filter disc, a temperature-variable amplification module, a light receiving light cone, a light collecting lens, a detection probe and a detection circuit board, wherein the light receiving light cone is arranged beside the transmission light filter disc and is connected with a receiving end of the temperature-variable amplification module through optical fibers, the optical fiber collecting lens is arranged beside the reception light filter disc and is connected with a transmitting end of the temperature-variable amplification module through optical fibers, one side, away from the optical fiber collecting lens, of the reception light filter disc is provided with the detection probe, and the detection probe is connected with the detection circuit board.

When a certain exciting light source on the light source component is aligned to the light receiving light cone, the light emitted by the light source is filtered to remove stray light by the emitting light filter with the corresponding wavelength and then is transmitted to the temperature-changing amplification module by the light receiving light cone. The light passes through the reagent sample in the temperature-variable amplification module, the temperature of which is kept stable between ninety and sixty more degrees and is cyclically switched so that the light passing through the excitation by the sample is enhanced. The light condenser lens receives the excited light, then filters stray light through the light receiving filter disc, and then the detection probe detects the stray light, and the excited light is analyzed through the detection circuit board. Whether the sample contains the corresponding substances is judged by judging whether the optical fiber is received or not, and the content of the corresponding substances in the sample is judged by judging the strength of the received optical fiber. The above steps are sequentially carried out by using exciting light with different wavelengths, and a plurality of substances in the sample can be respectively detected.

The bidirectional motor synchronously drives the light source component, the transmitting light filter disc and the receiving light filter disc to rotate, and then the transmitting light filters and the receiving light filters are in one-to-one correspondence. When the fiber condenser receives the excited light, the receiving optical filter disc needs to be adjusted independently. After the light source component is adjusted by the bidirectional motor, the receiving optical filter aligned with the optical fiber condenser corresponds to the wavelength of the received light. The utility model ensures that the adjustment of the light receiving and filtering disk is more convenient and accurate.

As a preferred scheme of the present invention, the light source assembly includes a light source plate, the light source plate is mounted on an output shaft of the bidirectional motor, a plurality of excitation light sources with different wavelengths are uniformly distributed on the light source plate, a plurality of emission light filters corresponding to the excitation light sources are arranged on the emission light filter disk, and a plurality of reception light filters corresponding to reception light amplified by the excitation light via the temperature-variable amplification module are arranged on the reception light filter disk. The six exciting light sources are uniformly distributed on the circumference of the light source plate, so that the determined exciting light sources can be aligned with the light receiving cone when the bidirectional motor drives the light source plate to rotate.

As a preferable aspect of the present invention, the number of the excitation light source, the emission light filter, and the reception light filter is six.

As a preferable scheme of the utility model, a rotary positioning sensor is further arranged beside the emission light filter disc, the rotary positioning sensor is electrically connected with a control circuit board, and the control circuit board is electrically connected with a bidirectional motor. The rotary positioning sensor can detect the rotating position of the light source plate, so that whether the corresponding exciting light source rotates in place or not is judged, and a signal is sent to the control circuit board. When the exciting light source is not rotated in place, the control circuit board controls the bidirectional motor to act, so that the exciting light source is rotated in place.

As a preferred scheme of the present invention, the optical signal emission detection module further includes an optical signal housing, and the bidirectional motor, the light receiving cone, the optical fiber condenser, the detection probe and the detection circuit board are all mounted on the optical signal housing.

As a preferred scheme of the present invention, the temperature-variable amplification module includes a suspended heating block, the light-receiving cone is connected to a receiving end of the suspended heating block through an optical fiber, the optical fiber condenser is connected to an emitting end of the suspended heating block through an optical fiber, and the peltier devices are disposed on both sides of the suspended heating block. The microfluidic PCR chip of the kit is inserted into the suspended heating block, and the suspended heating block and the Peltier can be controlled and stabilized between ninety-many degrees and sixty-many degrees to be kept stable and switched circularly, so that the light of laser after the exciting light passes through the sample reagent can be exponentially enhanced, and the detection of the excited light is facilitated.

The utility model also comprises a module shell, wherein the suspended heating block and the Peltier are arranged in the module shell.

As a preferable scheme of the present invention, heat sinks are mounted on both sides of the module case.

In a preferred embodiment of the present invention, a heat dissipation fan is installed on a side of the heat sink away from the peltier device.

The utility model has the beneficial effects that:

the bidirectional motor synchronously drives the light source component, the transmitting light filter disc and the receiving light filter disc to rotate, so that the transmitting light filters and the receiving light filters are in one-to-one correspondence. When the fiber condenser receives the excited light, the receiving optical filter disc needs to be adjusted independently. After the light source component is adjusted by the bidirectional motor, the receiving optical filter aligned with the optical fiber condenser corresponds to the wavelength of the received light. The utility model ensures that the adjustment of the light receiving and filtering disk is more convenient and accurate.

Drawings

FIG. 1 is a schematic diagram of the structure of a fully automatic PCR analysis system;

FIG. 2 is a schematic diagram of the structure of an analysis module;

FIG. 3 is an exploded view of an analysis module;

figure 4 is a partial block diagram of an analysis module cartridge;

FIG. 5 is a front view of the piston lifting module and the membrane-piercing post module;

FIG. 6 is a perspective view of the piston lifting module and the membrane sealing piercing post module;

figure 7 is a schematic view of the lower structure of an analysis module cartridge;

FIG. 8 is a schematic view of the structure of the kit;

FIG. 9 is a schematic structural diagram of a temperature-variable amplification module, a rotary valve driving module, a magnet lifting module and a thermal cracking module;

fig. 10 is a schematic structural view of a magnet lifting module;

FIG. 11 is a schematic diagram of a rotary valve drive module;

FIG. 12 is a schematic structural diagram of a temperature-variable amplification module;

FIG. 13 is a partial block diagram of the skeleton;

FIG. 14 is a schematic structural view of the present invention;

FIG. 15 is a partial block diagram of an optical signal emission detection module;

FIG. 16 is a sectional view of a temperature-variable amplification module.

In the figure, 1 — analysis module; 2-analysis module movement; 3-a lifting door assembly; 4-a base; 5-a human-computer interaction module; 6-a fan; 7-an interface board; 8-a foot pad; 9-indicator light board; 10-an indicator light guide post; 21-a backbone; 22-a control circuit board; 23-optical signal emission detection module; 24-a piston lifting module; 25-sealing a membrane puncture column module; 26-a temperature-variable amplification module; 27-rotary valve drive module; 28-magnet lifting module; 29-a pyrolysis module; 210-a kit; 31-a lift gate motor; 32-a lift gate screw; 33-a lifting table; 34-a lifting door; 35-positioning a stop block; 211-a limiting groove; 231-a bidirectional motor; 232-a light source assembly; 233-emission light filter wheel; 234-receive optical filter disk; 235-light-receiving cone; 236-fiber optic condenser; 237-detection probe; 238-detecting the circuit board; 239-a rotational positioning sensor; 2310-optical signal housing; 241-a lifting motor; 242-lifting screw rod; 243-lifting plate; 244-a push rod; 245-a spacer; 246-a positioning sensor; 247-a guide cylinder; 248-guide column; 251-a rotating plate; 252-a lancing frame; 253-piercing rod; 254-a return spring; 255-a first touch pressure sensor; 261-a suspended heating block; 262-Peltier; 263-module housing; 264-a heat sink; 265-a radiator fan; 271-a rotary electric machine; 272-a drive rod; 273-a positioning frame; 274-positioning plate; 275-valve position sensor; 276-a third touch pressure sensor; 281-a drive motor; 282-a magnet; 283-magnet slots; 284-a second touch pressure sensor; 2101-piston bore; 2102-puncture hole; 2103-microfluidic PCR chip; 2104-rotary valve operating orifice; 2321-light source board; 2322 — excitation light source; 2461-squeeze piston position sensor; 2462-median position sensor; 2463-puncture position sensor.

Detailed Description

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the utility model and are not to be construed as limiting the utility model.

As shown in fig. 12 and fig. 14 to fig. 15, the optical signal amplification detection apparatus of the full-automatic PCR analysis system of the present embodiment includes an optical signal emission detection module 23 and a temperature-varying amplification module 26, the optical signal emission detection module 23 includes an optical signal housing 2310, a bidirectional motor 231 is installed on the optical signal housing 2310, one output shaft of the bidirectional motor 231 is connected to a light source assembly 232 and a light emitting filter disk 233, and the other output shaft of the bidirectional motor 231 is connected to a light receiving filter disk 234; a light receiving cone 235 is arranged beside the emitting light filter disc 233, the light receiving cone 235 is connected with the receiving end of the variable temperature amplification module 26 through an optical fiber, a fiber collecting lens 236 is arranged beside the receiving light filter disc 234, the fiber collecting lens 236 is connected with the emitting end of the variable temperature amplification module 26 through an optical fiber, a detection probe 237 is arranged on one side of the receiving light filter disc 234 away from the fiber collecting lens 236, and a detection circuit board 238 is connected to the detection probe 237. The light source assembly 232 includes a light source plate 2321, the light source plate 2321 is installed on the output shaft of the bidirectional motor 231, six excitation light sources 2322 with different wavelengths are uniformly distributed on the light source plate 2321, six emission light filters corresponding to the excitation light sources 2322 are arranged on the emission light filter disc 233, and six reception light filters corresponding to reception light after the excitation light is amplified by the temperature-varying amplification module 26 are arranged on the reception light filter disc 234.

The bi-directional motor 231 drives the light source module 232, the emission light filter disk 233 and the reception light filter disk 234 to rotate, and when an excitation light source 2322 on the light source module 232 is aligned with the light receiving cone 235, the light emitted by the light source is filtered by the emission light filter with the wavelength corresponding to the light emitting light filter, and then is transmitted to the temperature varying amplification module 26 through the light receiving cone 235. The light passes through the reagent sample in the temperature-variable amplification module 26, and the temperature of the temperature-variable amplification module 26 is kept stable between ninety and sixty more degrees and cyclically switched so that the light passing through the excitation by the sample is enhanced. After receiving the excited light, the light condenser filters the stray light through the receiving light filter disc 234, and then the detection light is detected by the detection probe 237, and the excited light is analyzed through the detection circuit board 238. Whether the sample contains the corresponding substances is judged by judging whether the optical fiber is received or not, and the content of the corresponding substances in the sample is judged by judging the strength of the received optical fiber. The above steps are sequentially carried out by using exciting light with different wavelengths, and a plurality of substances in the sample can be respectively detected.

The bi-directional motor 231 synchronously drives the light source module 232, the transmitting light filter wheel 233 and the receiving light filter wheel 234 to rotate, so that the transmitting light filters and the receiving light filters are in one-to-one correspondence. When the fiber optic collection optic 236 receives the excited light, the receive filter wheel 234 needs to be adjusted individually. After the light source module 232 is adjusted by the bi-directional motor 231, the receiving optical filter aligned with the optical fiber condenser 236 corresponds to the wavelength of the received light. The present invention makes the adjustment of the receive optical filter wheel 234 more convenient and accurate.

Furthermore, a rotary positioning sensor 239 is further disposed beside the emission light filter disk 233, the rotary positioning sensor 239 is electrically connected to a control circuit board, and the control circuit board is electrically connected to the bi-directional motor 231. The rotational positioning sensor 239 can detect the rotational position of the light source plate 2321, thereby determining whether the corresponding excitation light source 2322 is rotated in place, and sending a signal to the control circuit board. When the excitation light source 2322 does not rotate in place, the control circuit board controls the bi-directional motor 231 to operate, so that the excitation light source 2322 rotates in place.

As shown in fig. 16, the temperature-variable amplification module 26 includes a module housing 263, a suspended heating block 261 is installed in the module housing 263, the light-receiving cone 235 is connected to a receiving end of the suspended heating block 261 through an optical fiber, the optical fiber condenser 236 is connected to an emitting end of the suspended heating block 261 through an optical fiber, and the peltier 262 is disposed on both sides of the suspended heating block 261. Heat sinks 264 are mounted on both sides of the module housing 263. The side of the heat sink 264 remote from the peltier 262 is fitted with a heat sink fan 265. The microfluidic PCR chip of the kit is inserted into the suspended heating block 261, and the suspended heating block 261 and the Peltier 262 can be controlled to be stable between ninety-many degrees and sixty-many degrees and can be kept stable and switched circularly, so that light of laser after the exciting light passes through the sample reagent can be exponentially enhanced, and the detection of the excited light is facilitated.

The optical signal emission detection module 23 and the temperature-variable amplification module 26 of the present invention are part of a full-automatic PCR analysis system. As shown in fig. 1 to 4, the full-automatic PCR analysis system for molecular diagnosis using a microfluidic chip includes a plurality of analysis modules 1, wherein each analysis module 1 includes an analysis module core 2; the analysis module core 2 comprises a framework 21, a control circuit board 22 is arranged on the framework 21, and an optical signal emission detection module 23, a piston lifting module 24, a membrane sealing puncture column module 25, a variable temperature amplification module 26, a rotary valve driving module 27, a magnet lifting module 28 and a heating cracking module 29 which are all electrically connected with the control circuit board 22 are respectively arranged on the framework 21; the kit 210 is provided with a microfluidic PCR chip 2103, the microfluidic PCR chip 2103 is respectively inserted into the temperature-changing amplification module 26, the magnet lifting module 28 and the heating and cracking module 29, the output end of the rotary valve driving module 27 is inserted into the kit 210, the output ends of the piston lifting module 24 and the membrane sealing puncture column module 25 are both inserted into the kit 210, and the optical signal emission detection module 23 is connected with the temperature-changing amplification module 26 through an optical fiber.

It should be noted that, in order to ensure that the system of the present invention is convenient to use, the present invention further includes a base 4, the plurality of analysis modules 1 are installed in the base 4, a human-computer interaction module 5 is further installed on the base 4, and the analysis modules 1 are respectively electrically connected with the human-computer interaction module 5.

The analysis module 1 further comprises a casing, the casing is provided with a fan 6, an interface board 7, a foot pad 8, an indicator lamp panel 9 and an indicator light guide post 10, except the analysis module movement 2, and the interface board 7 is electrically connected with a control circuit board 22 in the analysis module movement 2.

As shown in fig. 8 and 4, the cartridge 210 is provided with a plunger hole 2101 and a puncture hole 2102 at the upper side. The output end of piston lifting module 24 can be inserted into piston hole 2101, and the output end of membrane sealing puncture column module 25 can be inserted into puncture hole 2102. As shown in fig. 9, the microfluidic PCR chip 2103 is inserted into the temperature-variable amplification module 26, the magnet lifting module 28 and the thermal lysis module 29, respectively. The reagent cartridge 210 is further provided with a rotary valve operating hole 2104, and the output end of the rotary valve driving module 27 is inserted into the rotary valve operating hole 2104.

When the user uses the kit, the user firstly switches on the power supply and starts the kit for testing, after the user inputs the sample and the information of the kit 210, the sample is added into the sample position of the kit 210, then the cover of the kit 210 is closed, the corresponding detection position of the analysis system is inserted, and the test is started by clicking. All subsequent detection operations are automatically completed by the analysis system, and after the test is completed, the corresponding hatch door of the analysis system is opened, and the user waits for taking out the reagent kit 210 and clicks to complete the test to close the hatch door.

In the automatic operation process of the analysis system, the rotary valve driving module 27 drives the rotary valve to the position corresponding to the reagent cartridge 210, and the membrane sealing and puncturing module 25 punctures the liquid reagent sealing membrane in the reagent cartridge 210. The piston lifting module 24 then drives the rubber piston in the cartridge 210 to move, thereby effecting movement of the liquid. The rotary valve driving module 27 then drives the rotary valve to the desired position, draws the desired reagent through the piston and rotates the rotary valve to the desired position, squeezing the piston to expel the reagent, thus reciprocating the multiple reagent reactions. The magnetic lifting module 28 and the heating and cracking module 29 are matched to complete the cracking and extracting functions of the nucleic acid, and finally the temperature-variable amplification module 26 and the optical signal emission and detection module 23 are matched to complete the amplification and real-time optical signal detection of the nucleic acid. After the kit 210 is installed in place, the control circuit board 22 controls the optical signal emission detection module 23, the piston lifting module 24, the membrane sealing puncture column module 25, the temperature-variable amplification module 26, the rotary valve driving module 27, the magnet lifting module 28 and the pyrolysis module 29 to act, thereby realizing automatic molecular diagnosis.

As shown in fig. 4, in order to ensure that the reagent kit 210 is sealed during molecular diagnosis, the frame 21 is provided with a lifting door assembly 3, the lifting door assembly 3 comprises a lifting door motor 31 installed on the frame 21, an output shaft of the lifting door motor 31 is connected with a lifting door lead screw 32, the lifting door lead screw 32 is in threaded connection with a lifting platform 33, the lifting platform 33 is rotatably connected with a lifting door 34, and the lifting door 34 is lapped on the side wall of the frame 21. The door motor 31 can drive the door screw 32 to rotate, and the door screw 32 drives the lifting platform 33 to lift, so that the lifting platform 33 drives the lifting door 34 to lift. The lift gate 34 is raised before the reagent cartridge 210 is installed, and the lift gate 34 is lowered after the reagent cartridge 210 is installed. A positioning stopper 35 for limiting the elevating table 33 is further mounted on the frame 21.

The specific structures of the piston lifting module 24 and the seal piercing column module 25 are described below:

as shown in fig. 5 and 6, the piston lifting module 24 includes a lifting motor 241 mounted on the frame 21, an output end of the lifting motor 241 is connected with a lifting screw 242, the lifting screw 242 is connected with a lifting plate 243 through a thread, the lifting plate 243 is connected with a push rod 244 for pushing a rubber piston in the reagent kit 210 to move, and the push rod 244 is inserted into a piston hole 2101 of the reagent kit 210. The elevating motor 241 drives the elevating screw 242 to rotate, and the elevating screw 242 drives the elevating plate 243 to ascend or descend. When the lifting plate 243 descends, the pushing force on the lifting plate 243 extends into the reagent box 210 and drives the rubber piston in the reagent box 210 to move, so that the required reagent can be extracted to a required position by driving the piston to act.

In order to ensure the lifting plate 243 to move stably, two guide rods are further installed on the framework 21, two guide cylinders 247 are fixed on the lifting plate 243, and the guide cylinders 247 are sleeved on the guide posts 248. When the elevating plate 243 is driven by the elevating screw 242, the elevating plate 243 is restricted by the two guide posts 248 to be rotated, and the elevating plate 243 can be smoothly linearly elevated.

The membrane sealing and piercing module 25 includes a rotating plate 251, the rotating plate 251 is hinged to the frame 21, one end of the rotating plate 251 is lapped on the lifting plate 243, the other end of the rotating plate 251 is hinged to a piercing frame 252, and the piercing frame 252 is connected with a plurality of piercing rods 253 for piercing the liquid reagent sealing membrane in the reagent box 210. When the lifting plate 243 is lifted, the rotating plate 251 is pushed to rotate, so that the puncturing rod 253 on the rotating plate 251 can puncture the liquid reagent sealing membrane in the reagent kit 210.

When puncturing rod 253 is lifted, first touch sensor 255 is attached to frame 21 in order to limit puncture rack 252. When the puncture rack 252 rises to press the first touch sensor 255, the first touch sensor 255 sends a signal to the control circuit board 22, and the control circuit board 22 controls the lifting motor 241 to stop.

A plurality of return springs 254 are coupled to piercing carriage 252 for biasing piercing carriage 252. When the lifting plate 243 is lowered, the lifting plate 243 is separated from the rotating plate 251, and the return spring 254 pushes the puncture rack 252 and the puncture rod 253 upwards in the process of returning, so that the puncture rod 253 is prevented from staying in the reagent kit 210.

A positioning plate 245 is fixed on the lifting plate 243, three positioning sensors 246 are arranged on the moving path of the positioning plate 245, and the positioning sensors 246 are electrically connected with the control circuit board 22. Position sensors 246 include squeeze piston position sensor 2461, neutral position sensor 2462, and pierce position sensor 2463. When the positioning tab 245 moves to the position of the squeeze piston position sensor 2461, the push rod 244 moves to the lowermost end and the reagent in the piston is completely squeezed out. When the positioning tab 245 moves to the neutral sensor 2462 position, the piercing rod 253 and the push rod 244 are both separated from the reagent cartridge 210. When the positioning tab 245 is moved to the puncturing position sensor 2463, the puncturing rod 253 is in a position to puncture the liquid reagent sealing membrane. The squeeze piston position sensor 2461, the middle position sensor 2462 and the puncture position sensor 2463 are all electrically connected to the control circuit board 22, and when any one of the positioning sensors 246 is triggered by the positioning piece 245, the positioning sensor 246 sends a signal to the control circuit board 22, and the control circuit board 22 controls the stop or start of the lifting motor 241.

The specific structure of the lower part of the analysis module cartridge 2 is described below:

as shown in fig. 7 to 10, the magnet lifting module 28 includes a driving motor 281 installed on the frame 21, a magnet 282 is connected to an output shaft of the driving motor 281, a magnet slot 283 is disposed on the magnet 282, and the microfluidic PCR chip 2103 passes through the magnet slot 283. When the driving motor 281 drives the magnet 282 to rise, the microfluidic PCR chip 2103 passes through the magnet slot 283, so that the separation of the magnetic beads inside the kit 210 is realized.

A second touch sensor 284 for detecting whether the magnet 282 is lowered in place is further mounted on the mounting bracket of the driving motor 281, and the second touch sensor 284 is electrically connected to the control circuit board 22. When the magnet 282 descends and presses the second touch sensor 284, the second touch sensor 284 sends a signal to the control circuit board 22, and the control circuit board 22 controls the driving motor 281 to stop.

Specifically, as shown in fig. 11, the rotary valve driving module 27 includes two rotary motors 271 mounted on the frame 21 and respectively disposed at two sides of the reagent cartridge 210, an output shaft of the rotary motor 271 is connected to a driving rod 272 for driving the rotary valves, and the driving rod 272 is inserted into the rotary valve operating hole 2104 of the reagent cartridge 210. The rotary motor 271 drives the driving rod 272 to rotate, and then the driving rod 272 drives the rotary valve to rotate to the corresponding position, thereby realizing the selection of different flow channels or reagents.

The framework 21 is further provided with a positioning frame 273, the driving rod 272 is provided with a positioning disc 274, the positioning disc 274 is provided with a plurality of positioning grooves, the positioning disc 274 is sleeved with a valve position sensor 275, and the valve position sensor 275 is arranged on the positioning frame 273. The valve position sensor 275 on the positioning frame 273 determines the open position of the rotary valve by detecting the rotation angle of the positioning plate 274, facilitating automatic control.

The third touch sensor 276 is mounted on the inside of the positioning frame 273, and when the reagent cartridge 210 is mounted and the reagent cartridge 210 presses the third touch sensor 276, the third touch sensor 276 transmits a mounting-in-place signal to the control circuit board 22.

As shown in fig. 9, a slot for inserting the microfluidic PCR chip 2103 is disposed on the pyrolysis module 29, and the pyrolysis module 29 is used for controlling the temperature to heat the chip pyrolysis chamber.

As shown in fig. 13, the frame 21 is provided with a stopper groove 211 for stopping the puncture rack 252. The limiting groove 211 can limit the puncture frame 252, and ensure that the puncture frame 252 can accurately move along a straight line, thereby ensuring that the puncture rod 253 can accurately puncture the sealing film.

The utility model is not limited to the above alternative embodiments, and any other various forms of products can be obtained by anyone in the light of the present invention, but any changes in shape or structure thereof, which fall within the scope of the present invention as defined in the claims, fall within the scope of the present invention.

Claims (9)

1. The light signal amplification detection device of the full-automatic PCR analysis system is characterized by comprising a light signal emission detection module (23) and a variable temperature amplification module (26), wherein the light signal emission detection module (23) comprises a bidirectional motor (231), one output shaft of the bidirectional motor (231) is connected with a light source component (232) and a light emitting filter disc (233), and the other output shaft of the bidirectional motor (231) is connected with a light receiving filter disc (234); the light-receiving optical filter disc is characterized in that a light-receiving optical cone (235) is arranged beside the emitting light filter disc (233), the light-receiving optical cone (235) is connected with a receiving end of the variable-temperature amplification module (26) through an optical fiber, a fiber collecting lens (236) is arranged beside the light-receiving optical filter disc (234), the fiber collecting lens (236) is connected with a transmitting end of the variable-temperature amplification module (26) through an optical fiber, a detection probe (237) is arranged on one side, away from the fiber collecting lens (236), of the light-receiving optical filter disc (234), and a detection circuit board (238) is connected onto the detection probe (237).

2. The optical signal amplification detection device of the full-automatic PCR analysis system according to claim 1, wherein the light source assembly (232) comprises a light source plate (2321), the light source plate (2321) is installed on the output shaft of the bidirectional motor (231), a plurality of excitation light sources (2322) with different wavelengths are uniformly distributed on the light source plate (2321), a plurality of emission light filters corresponding to the excitation light sources (2322) are arranged on the emission light filter disc (233), and a plurality of reception light filters corresponding to the reception light amplified by the excitation light through the temperature-variable amplification module (26) are arranged on the reception light filter disc (234).

3. The optical signal amplification detection device of the full-automatic PCR analysis system according to claim 2, wherein the number of the excitation light source (2322), the number of the emission light filter and the number of the receiving light filter are all six.

4. The light signal amplification detection device of the full-automatic PCR analysis system according to claim 1, wherein a rotary positioning sensor (239) is further disposed near the emission light filter disc (233), the rotary positioning sensor (239) is electrically connected to a control circuit board, and the control circuit board is electrically connected to the bi-directional motor (231).

5. The optical signal amplification detection device of the full-automatic PCR analysis system according to claim 1, wherein the optical signal emission detection module (23) further comprises an optical signal housing (2310), and the bidirectional motor (231), the light receiving cone (235), the optical fiber condenser (236), the detection probe (237) and the detection circuit board (238) are all mounted on the optical signal housing (2310).

6. The optical signal amplification detection device of the full-automatic PCR analysis system according to claim 1, wherein the temperature-variable amplification module (26) comprises a suspended heating block (261), the light-receiving cone (235) is connected with the receiving end of the suspended heating block (261) through an optical fiber, the optical fiber condenser (236) is connected with the emitting end of the suspended heating block (261) through an optical fiber, and the Peltier (262) is disposed on both sides of the suspended heating block (261).

7. The optical signal amplification detection device of the full-automatic PCR analysis system according to claim 6, further comprising a module housing (263), wherein the suspended heating block (261) and the Peltier (262) are installed in the module housing (263).

8. The optical signal amplification detection device of the full-automatic PCR analysis system according to claim 7, wherein the module casing (263) is provided with heat sinks (264) at both sides.

9. The optical signal amplification detection device of the full-automatic PCR analysis system according to claim 8, wherein a heat dissipation fan (265) is installed on a side of the heat sink (264) far away from the Peltier (262).

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