CN115703989A

CN115703989A - Nucleic acid quick detection equipment

Info

Publication number: CN115703989A
Application number: CN202110968414.3A
Authority: CN
Inventors: 岂源
Original assignee: Beijing Qingfengtang Pharmaceutical Technology Co ltd
Current assignee: Beijing Qingfengtang Pharmaceutical Technology Co ltd
Priority date: 2021-07-30
Filing date: 2021-08-23
Publication date: 2023-02-17
Also published as: CN215667945U; CN115703991A; CN215906211U; CN115704049A; CN215906212U; CN215757272U; CN115786097A; CN215667988U; CN115703990A

Abstract

The invention discloses a nucleic acid rapid detection device, wherein an isothermal amplification instrument comprises a bearing part, a heating part, a mounting frame body and a shell, wherein the bearing part and the heating part are arranged in the shell through the mounting frame body; the bearing part is provided with at least one bearing cavity position for placing a sample to be tested; the heating part is positioned below the bearing part and electrically heats the bearing part. The nucleic acid rapid detection equipment, the reactor and the isothermal amplification instrument are matched for use, so that the problem of uncovering pollution in the amplification method in the prior art can be solved, the reactor is disposable, the reaction can be directly carried out after a sample is added, uncovering is not needed in the reaction process, multiple times of liquid adding is not needed, the whole nucleic acid detection can be realized without a professional laboratory, no aerosol pollution exists, and the detection can be completed by processing the reactor.

Description

Nucleic acid quick detection equipment

Technical Field

The invention relates to the technical field of nucleic acid detection (DNA or RNA), in particular to a nucleic acid rapid detection device.

Background

Nucleic acid detection, as a method having high sensitivity and specificity, has been widely used in many fields such as disease diagnosis, food safety, infectious disease control, and the like. Detection of specific nucleic acid sequences in a simple manner can confer greater value in point-of-care (point-of-care) diagnostics and in point-of-care pathogen detection.

PCR (polymerase chain reaction) is a molecular biology technique for amplifying and amplifying a specific DNA fragment, which can be regarded as special DNA replication in vitro, and the biggest characteristic of PCR is that a trace amount of DNA can be greatly increased. However, PCR, a classical nucleic acid detection method, its inherent denaturation-renaturation-extension cycle, requires that it necessarily requires thermocycler equipment as a support, and professional laboratories also serve as a necessary condition because of aerosol contamination issues. Among them, the PCR extension technology platform, particularly the quantitative PCR (qPCR) method, is the most widely used pathogen detection method and is considered as a new gold standard test. qPCR provides a much shorter sample-to-result time (3 to 5 hours). However, although qPCR is widely accepted, it is limited by relying on standard reference substances (standard curves) for quantification. Unreliable and inconsistent commercial standard reference substances may also affect the accuracy of qPCR quantification. In addition, qPCR is susceptible to inhibition by naturally occurring substances in environmental samples (e.g., heavy metals and organic matter), leading to inaccurate or false negative results in target quantification. Therefore, the application of PCR in the fields of point-of-care rapid diagnosis (POCT), on-site rapid detection and the like is greatly limited. Compared to qPCR, recent digital PCR techniques have proven to be more robust solutions for the detection of microbial pathogens in environmental samples. Digital PCR is based on partitioning (partioning) and poisson statistics, so there is no need to compare external quantification standards to quantify samples of unknown concentration. However, implementing digital PCR methods for use with point-of-use applications (point-of-use applications) can be challenging. This is because digital PCR requires expensive instrumentation (i.e., bio-rad droplet digital PCR), a full-scale laboratory environment, and trained technicians to perform the assay. These factors severely limit the accessibility and applications of digital PCR in resource-limited contexts.

To overcome these disadvantages, a large class of new methods for isothermal nucleic acid amplification has emerged, with LAMP being the most interesting and promising method.

Loop-mediated isothermal amplification (LAMP) is an alternative PCR nucleic acid amplification method developed by chemical Co., ltd. Of Japan Rong Yan in 2000. It is characterized by that 4 specific primers are designed according to 6 regions of target gene, under the action of strand displacement DNA polymerase (Bst DNA polymerase), the amplification is implemented at constant temp. of 60-65 deg.C for about 15-60 min ⁹ ～10 ¹⁰ The double nucleic acid amplification has the characteristics of simple operation, strong specificity, easy detection of products and the like. LAMP, as a molecular biology detection technology, has the characteristics of high specificity, high sensitivity, simplicity, convenience and low cost, and is widely used for diagnosis of clinical diseases, qualitative and quantitative detection of epidemic bacteria or viruses, sex identification of animal embryos and gene chips.

Thus, LAMP detection is a rapid, simplified, low cost assay for detecting microorganisms to provide molecular assays outside of a centralized laboratory, for example, where on-site point-of-use testing of environmental water in resource-limited locations is required.

LAMP detection is performed under isothermal conditions, which can be maintained in different instruments, such as a thermocycler and a water bath. The apparatus enables amplification of DNA/cDNA from a sample by heating a detection chamber inside the device to detect pathogens.

The isothermal amplification instrument performs amplification reaction under the constant temperature condition by using the strand displacement type DNA polymerase, can realize 109-1010 times of amplification within 15-60 minutes, can generate a large amount of amplification products, namely magnesium pyrophosphate white precipitate, and can judge whether the target gene exists by observing the existence of the white precipitate by naked eyes. The LAMP method has the advantages of high specificity and high sensitivity, is very simple to operate, has low requirements on instruments in the application stage, can realize reaction by using a simple constant temperature device, is very simple in result detection, can directly observe white precipitates or green fluorescence by naked eyes, does not need to carry out gel electrophoresis observation results unlike the common PCR method, and is a method suitable for rapid detection on site and in basic level.

Because of the limitations of nucleic acid detection reagents and detection equipment based on amplification, the problem of extraction of nucleic acid or other samples to be detected cannot be solved by amplification operation in the existing detection, multiple uncovering is also needed in the amplification process, especially when an eight-connected tube is used as a reactor, and operation in a professional PCR laboratory is also needed to avoid pollution, so that the nucleic acid detection in the prior art cannot realize field sampling and field detection, especially no reactor capable of directly completing reaction at one time after directly adding a sample is available, the traditional eight-connected tube or EP tube (centrifugal tube) is still adopted, which is an important toggle for the nucleic acid detection not to be well applied to POCT and the development and application of pathogenic microorganisms. In addition, the isothermal amplification instrument can be applied to the fields of pathogenic microorganisms, species identification, animal diseases, transgenosis and the like, but the traditional isothermal amplification instrument is large in size and inconvenient to carry, so that the isothermal amplification instrument can only be used in a laboratory.

Disclosure of Invention

The technical problem to be solved by the invention is as follows: aiming at the technical problems in the prior art, the invention provides the nucleic acid rapid detection equipment which can be directly added with a sample to be detected without opening a cover, so that the whole nucleic acid detection is realized without a PCR laboratory and aerosol pollution.

In order to solve the technical problems, the technical scheme provided by the invention is as follows:

a nucleic acid rapid detection device comprises an isothermal amplification instrument and a detection device, wherein the isothermal amplification instrument comprises a bearing part, a heating part, a mounting frame body and a shell, and the bearing part and the heating part are arranged in the shell through the mounting frame body; the bearing part is provided with at least one bearing cavity position for placing a sample to be tested; the heating part is positioned below the bearing part and electrically heats the bearing part.

As a further improvement of the above technical solution:

preferably, the heating part comprises a heating plate and a plurality of heating sheets, the heating sheets are positioned below the heating plate, and the heating plate is fixed at the bottom of the bearing part. The heating part heats in the reaction process, so that the reaction environment is maintained at a constant temperature of 60-65 ℃, and the nucleic acid amplification is rapidly realized.

Preferably, the isothermal amplification instrument further comprises a vibration part and a driving part, wherein the vibration part is arranged below the bearing part, the vibration part drives the bearing part to vibrate, and the vibration part is driven by the driving part. The vibration part drives the bearing part to vibrate so that the sample to be detected and the reaction system vibrate and are uniformly mixed.

Preferably, vibration portion is equipped with the vibration deformation board, it is located the vibration deformation board top to bear the weight of the position, vibration portion still is equipped with the mounting panel, the mounting panel is equipped with at least two, is located the relative both sides of vibration deformation board respectively, the mounting panel is connected with the installation support body, there is the clearance between vibration deformation board and the installation support body, can not cause the influence to the installation support body when vibration deformation board vibrates.

Preferably, the device is further provided with a reactor, the reactor comprises a sample adding part, a sample part and a reaction part which are connected in sequence, wherein the sample adding part, the sample part and the reaction part are movably connected to realize a sealing state, the sample adding part is of a piston structure, a sample preserving fluid is pre-filled in the sample part, and a reaction system is filled in the reaction part; the connecting part of the reaction part and the sample part is provided with micropores, the pore diameter of each micropore is not more than the capillary length of the sample liquid or the sample preservation liquid, in other words, the surface tension of the liquid at each micropore is more than the gravity thereof; under the sealed state of the reactor, the sample adding part moves towards the reaction part by external force to realize that the sample liquid passes through the micropores and is pressed into the reaction part under the sealed state.

Preferably, at least one reaction chamber is arranged in the reaction part, an independent reaction system is preset in the reaction chamber, simultaneous detection of multiple detection items for the same sample can be realized, a micropore is arranged at the orifice of each reaction chamber, and liquid cannot flow into the reaction chamber from the sample part under the condition of no external force.

Preferably, the reaction chambers are closed at the ports close to the sample part, each reaction chamber is in an independent sealing state before reaction, and the ports are closed through a sealing plug or a sealing film. Because a sealing film or a sealing plug is provided, the reaction chamber can be kept in a sealed state even in the case where the threaded joint of the reaction part and the sample part is unscrewed.

Preferably, the reaction part is provided with a shunt plug, the shunt plug is made of flexible materials, the shunt plug is located at the joint of the reaction part and the sample part, the reaction cavity is independently sealed through the shunt plug, and the shunt plug is arranged in the micropore.

Preferably, one surface of the shunt plug protrudes towards the reaction cavity to seal the cavity opening, the micropores penetrate through the protrusion, and one surface of the shunt plug facing the sample part is a smooth surface.

Preferably, the pore diameter of the micropores is 0.3-0.6 mm.

Preferably, the reaction part is provided with an inner reaction tube and an outer sleeve which are sleeved, the inner reaction tube is clamped in the outer sleeve, and the outer sleeve is provided with a thread which is in coordination threaded connection with the sample part.

Preferably, the sample adding part comprises a sleeved concave plug and a sample adding cap, a rigid protruding part inserted into the concave plug and sleeved with the concave plug is arranged in the sample adding cap, the sample adding cap is also provided with a threaded connection section in coordination and threaded connection with the sample part, so that the sample adding part and the sample part can be hermetically connected, and sample liquid can enter the reaction part by pressurizing the sample part through unidirectional rotation.

Preferably, the sample adding part is further provided with a limiting part, and the limiting part is used for limiting the rotation of the sample adding part.

Preferably, the sample part is a coaxial sleeve, wherein the inner tube is a hollow tube, the inner diameter of the hollow tube is in sealing fit connection with the concave plug, and one end of the outer tube is provided with a thread which is in coordination threaded connection with the sample adding part; when the reactor is sealed, one section of the inner tube is positioned in the gap between the concave plug and the sample adding part; the sample preservation solution is pre-arranged in the cavity of the inner tube before reaction.

Preferably, one end of the outer tube of the sample part, which is close to the reaction part, is provided with a thread which is in coordination threaded connection with the reaction part, and the reaction part is not communicated with the outer tube.

Preferably, the sample part is further provided with a sealing member disposed between the reaction part and the sample part, the sealing member being used to seal the sample preservation solution, so that the inner cavity of the sample part can be in a sealed state even when the threaded joint of the reaction part and the sample part is unscrewed.

Preferably, the apparatus further comprises a photographing part for photographing the developed sample; the portion of shooing sets up in the shell through the installation support body, the portion of shooing includes the camera, shoots the box, it is equipped with the trench of placing reaction back sample to shoot the box, it can stretch out or retract in the shell to shoot the box.

Preferably, the portion of shooing still includes slider and slide rail, mounting bracket board and slider fixed connection, the slider cooperatees with the slide rail, but relative slip, shoot box fixed mounting in the one end of slide rail, the slide rail can move the relative slider, and the drive is shot the box and is stretched out or retract the shell.

Compared with the prior art, the nucleic acid rapid detection equipment provided by the invention has the following advantages:

(1) According to the nucleic acid rapid detection equipment, the amplification instrument can simultaneously carry out multiple joint detections on the same sample; heating the reaction by a heating part in the reaction process to maintain a constant temperature environment required by the amplification reaction; the sample solution and the reaction system are uniformly vibrated by the vibrating part.

(2) The nucleic acid rapid detection equipment, the reactor and the amplification instrument are matched for use, so that the problem of aerosol pollution caused by uncapping sample injection or liquid adding in the amplification method in the prior art can be solved, the reactor is disposable, the reaction can be directly carried out after a sample is added, uncapping is not needed in the reaction process, multiple times of liquid adding is not needed, the whole nucleic acid detection can be realized without a professional laboratory, no aerosol pollution exists, and the detection can be completed by processing the reactor.

(3) The nucleic acid rapid detection equipment can be realized by matching simple heating equipment with the nucleic acid detection method and the reactor aiming at public health events, does not need to be operated by professionals, has clear and easily-judged result, is suitable for the requirements of various medical detection scenes at home and abroad at present, and particularly greatly improves the molecular diagnosis capability of relatively laggard areas.

Drawings

FIG. 1 is a schematic diagram of a usage status of an embodiment of the present invention.

FIG. 2 is a schematic diagram of the explosion structure of the isothermal amplification apparatus of the present invention.

FIG. 3 is a schematic view of the structure of the reactor of the present invention.

The reference numbers in the figures illustrate:

1. a bearing part; 11. a load bearing cavity; 2. a heating section; 21. heating plates; 22. a ceramic heating plate; 3. a vibrating section; 31. a vibration deformable plate; 32. mounting a plate; 4. a drive section; 41. a vibration motor; 42. an in-out motor; 5. a photographing part; 51. a camera; 52. a photographing box; 53. a slider; 54. a slide rail; 55. a connecting plate; 6. a housing; 61. a cover; 7. installing a frame body; 71. an upper mounting seat; 72. a support; 73. a base; 74. a connecting seat; 75. mounting blocks; 8. a reactor; 81. a sample section; 811. an outer tube; 812. an inner tube; 813. a seal member; 82. a sample addition part; 821. a concave plug; 822. a sample adding cap; 823. tearing the pull ring; 83. a reaction section; 831. an outer sleeve; 832. an inner reaction tube; 833. a reaction chamber; 834. a shunt plug; 8341. a boss member; 8342. and (4) micro-pores.

Detailed Description

The present invention will be described more fully hereinafter with reference to the following examples. The following examples are illustrative only and are not to be construed as limiting the invention.

The experimental procedures in the following examples are conventional unless otherwise specified. The experimental materials used in the following examples were all commercially available unless otherwise specified.

Fig. 1 to 3 show an embodiment of the nucleic acid rapid detection device of the present invention, which includes a reactor 8 and an isothermal amplification apparatus, the isothermal amplification apparatus is provided with a plurality of loading cavity sites 11, a sample to be detected is placed in the reactor 8, and the isothermal amplification apparatus can perform a mixed detection on different samples simultaneously.

In this embodiment, the isothermal amplification instrument includes a bearing portion 1, a heating portion 2, a vibration portion 3, a driving portion 4, a photographing portion 5, a mounting frame body 7 and a housing 6, and the bearing portion 1, the heating portion 2, the vibration portion 3, the driving portion 4 and the photographing portion 5 are disposed in the housing 6 through the mounting frame body 7. The mounting frame body 7 is of a special-shaped plate structure, the bearing part 1, the heating part 2 and the vibrating part 3 are mounted at the upper end of the special-shaped plate, and the driving part 4 is mounted in a cavity of the special-shaped plate and is positioned below the vibrating part 3; the photographing part 5 is installed at one side of the special-shaped plate.

In this embodiment, the mounting frame body 7 includes an upper mounting seat 71, a base 73 and two supports 72, four corner ends of the upper mounting seat 71 are respectively provided with a protruding mounting block 75, and the upper mounting seat 71 is provided with a mounting hole. Two supports 72 are respectively arranged at two sides of the upper mounting seat 71, and the bottom of the support 72 is fixed on the base 73.

In this embodiment, the bearing portion 1 is located to bearing chamber position 11, and bearing portion 1 is equipped with a loading board and a plurality of location section of thick bamboo, and a location section of thick bamboo is fixed in on the loading board, and loading board and a location section of thick bamboo adopt heat conduction and non-flexible material preparation, and bearing chamber position 11 is enclosed by a loading board and a location section of thick bamboo and establishes and form, and bearing chamber position 11 arranges according to the order array, and a reactor 8 can be placed to every bearing chamber position 11. The housing 6 is provided with a cover 61, the cover 61 being positioned above the carrier 1, the cover 61 being openable for insertion or removal of the reactor 8.

In this embodiment, heating portion 2 includes heating plate 21 and a plurality of heating plate, and the heating plate adopts ceramic heating plate 22 in this embodiment, and ceramic heating plate 22 is located heating plate 21 below, and heating plate 21 is fixed in the bottom of loading board, and ceramic heating plate 22 is through loading board and the heating of a location section of thick bamboo to reactor 8 bulk heating, can the even heating, control reaction temperature that simultaneously can be more accurate.

In this embodiment, the vibration portion 3 is provided with the vibration deformation plate 31, the ceramic heating sheet 22 is mounted on the vibration deformation plate 31, the vibration deformation plate 31 is mounted on the upper mounting seat 71, the vibration deformation plate 31 is provided with four mounting plates 32, and each mounting plate 32 is fixedly connected with one mounting block 75. A gap is provided between the side surface of the vibration deformation plate 31 and the mounting plate 32, so that the impact of the vibration deformation plate 31 on the upper mounting seat 71 can be reduced.

In this embodiment, the driving portion 4 includes a vibration motor 41 and an in-out motor 42, an eccentric vibrator is disposed on a driving shaft of the vibration motor 41, the vibration motor 41 is mounted below the upper mounting seat 71 and fixed on the base 73, the driving shaft of the vibration motor 41 passes through the mounting hole of the upper mounting seat 71, and the eccentric vibrator drives the vibration deformation plate 31 to drive the bearing portion 1 to vibrate. The in-out motor 42 is fixed to the base 73 and drives the photographing section 5.

In this embodiment, the photographing part 5 includes a camera 51, a photographing box 52, a sliding block 53, a sliding rail 54 and a connecting plate 55, the upper mounting plate 32 is provided with a connecting seat 74, the sliding block 53 is fixedly connected with the connecting seat 74, and the sliding block 53 is matched with the sliding rail 54 and can slide relatively. The connecting plate 55 is fixed to the bottom end of the sliding rail 54, the connecting plate 55 is provided with a sliding slot, and the driving shaft of the in-out motor 42 is fixedly connected with an eccentric connecting rod which can slide in the sliding slot of the connecting plate 55. The camera 51 is fixed on the base 73 by a camera mount, and the photographing box 52 is fixedly mounted on one end of the slide rail 54. The in-out motor 42 moves the slide rail 54 relative to the slide block 53 via the eccentric link to drive the photographing box 52 to extend out of the housing 6. The housing 6 is provided with a corresponding openable and closable opening. The cassette 52 is provided with a slot for placing the reactor. The photo cassette 52 is moved out of the housing 6 a distance to expose the housing 6 in a slot to allow the reactor to be placed.

In this embodiment, the photographing box 52 extends out of the housing 6 during photographing, and is placed into the reactor after the reaction is completed, then the photographing box 52 is moved into the housing 6, the camera 51 photographs, and after photographing, the photographing box 52 extends out of the housing 6, and the reactor after photographing is taken out, and is placed into the next reactor for continuing photographing. In fact, the photographing and the reaction can be performed simultaneously, that is, after the reaction is performed for the first time, the second batch of reactors can be placed in the bearing part 1, so that the detection speed is increased.

The isothermal amplification instrument of the embodiment is also provided with a cooling unit for cooling the motor, and a host machine for controlling the operation of each part. The cooling unit is provided with a fan for cooling the motor. The isothermal amplification instrument is provided with a control system, and the control system comprises a detection module, a conversion module, a processing module and a display module. The detection module is provided with a temperature sensor and is used for detecting the temperature of the bearing part 1 and transmitting detection data to the processing module; the conversion module comprises a DC-DC converter; the processing module is used for displaying the detected temperature data on the display module. The camera 51 is electrically connected to the processing module and the picture is displayed on the display module. The cooling unit and the control system are arranged according to the mechanical structure part of the isothermal amplification instrument and adopt conventional components capable of achieving corresponding control effects,

the device is also provided with a reactor 8 matched with the isothermal amplification instrument for use, the reactor 8 comprises a sample adding part 82, a sample part 81 and a reaction part 83 which are connected in sequence, wherein the sample adding part 82 and the sample part 81, and the sample part 81 and the reaction part 83 are movably connected to realize a sealing state, the sample adding part 82 is of a piston structure, a sample preserving fluid is pre-loaded in the sample part 81, and a reaction system is loaded in the reaction part 83; a micropore 8342 is arranged at the joint of the reaction part 83 and the sample part 81, and the pore diameter of the micropore 8342 is not more than the capillary length of the sample liquid or the sample preservation liquid; the surface tension of the liquid at the micropores is greater than its gravity. In this embodiment, the pore diameter of the micropores is 0.3 to 0.6mm. In the sealed state of the reaction vessel 8, the sample addition part 82 moves toward the reaction part 83 by an external force, and the sample liquid is pressed into the reaction part 83 through the micropores 8342 in the sealed state. For the convenience of being placed in the isothermal amplification apparatus, the reactor 8 is integrally of a circular tube type structure, and particularly preferably made of a transparent plastic material.

In this embodiment, a plurality of reaction chambers 833 are provided in the reaction section 83, independent reaction systems are provided in the reaction chambers 833 in advance, simultaneous detection of a plurality of detection items for the same sample can be achieved, and the end portion of each reaction chamber 833 near the junction is provided with a micropore 8342, so that liquid cannot flow into the reaction chamber 833 from the sample section 81 without external force.

In this embodiment, the reaction portion 83 is provided with an inner reaction tube 832 and an outer sleeve 831 which are sleeved with each other, the inner reaction tube 832 is clamped in the outer sleeve 831, one end of the outer sleeve 831 is provided with an internal thread which is in coordination threaded connection with the sample portion 81, the reaction portion 83 is provided with a shunt plug 834, the shunt plug 834 is made of a flexible material, and the reaction portion 83 is made of a transparent rubber material. Shunt plug 834 is located the junction of reaction chamber 833 and sample portion 81, and one section upper portion of reaction portion 83 is the internal thread section, and the position of reaction chamber 833 is not located the junction of internal thread, and internal thread junction and reaction chamber 833 have been cut apart to shunt plug 834 promptly, and one side of shunt plug 834 is protruding in order to seal the accent in reaction chamber 833, and every is protruding to have a micropore 8342, and micropore 8342 runs through the arch, and the one side that shunt plug 834 faces sample portion 81 is the smooth surface. Reaction chamber 833 is sealed independently by a shunt plug 834. Before reaction, a reaction system is pre-buried in each reaction cavity 833, and the reaction system is sealed and buried, for example, a material which is not compatible with water and can change the form along with the change of temperature is used for sealing the reaction system. Each reaction chamber 833 can be provided with a different reaction system for detecting different items. Since the reactor 8 is small and compact as a whole, it is preferable to provide 4 reaction chambers 833.

In this embodiment, the outer sleeve 831 is a hollow tube, the inner reaction tube 832 is clamped in the outer sleeve 831, the inner reaction tube 832 is provided with one or more than one protruding ridge for positioning, the outer sleeve 831 is provided with a corresponding groove, and the inner reaction tube 832 and the outer sleeve 831 can be integrally formed or can be manufactured separately. In practice, because the reactor 8 is small, the outer sleeve 831 and the inner reaction tube 832 are typically manufactured in two parts and then assembled for ease of manufacture and ease of handling for embedding the reaction system.

In this embodiment, at least one protrusion or a groove is formed on the outer surface of the outer sleeve 831, i.e., the outer surface of the reaction portion 83, for positioning the reactor 8 when it is placed in the loading cavity 11 of the isothermal amplification apparatus, and the loading cavity 11 is provided with a groove or a protrusion.

In this embodiment, the sample adding part 82 includes a sleeved concave plug 821 and a sleeved sample adding cap 822, one section of the sample adding cap 822 connected with the sample part 81 is provided with an external thread connecting section, a rigid protruding part 8341 inserted into the sleeved concave plug 821 is arranged in the sample adding cap, the protruding part 8341 is cylindrical or cylindrical, the protruding part 8341 and the external thread connecting section are of concentric rings, and an annular cavity is arranged between the protruding part 8341 and the external thread connecting section. The concave plug 821 is made of transparent rubber materials, the concave plug 821 adopts a piston structure with a cylindrical cavity arranged inside, a circular ring extending outwards is arranged at the cavity opening of the top of the piston, the outer diameter of the circular ring is the same as that of the annular cavity, the diameter of the piston head at the other end is slightly larger than that of the inner cavity of the sample part 81, and the function of the piston head in the inner cavity of the sample part 81 is achieved. When the concave plug 821 is sleeved on the sample adding cap 822, the head part of the piston protrudes out of the sample adding cap 822, so that the sample adding part 82 and the sample part 81 can be connected in a sealing manner, and the sample liquid can enter the reaction part 83 by pressurizing the sample part 81 through rotating the sample adding part 82 in a single direction.

In this embodiment, the sample portion 81 is a coaxial sleeve, the inner tube 812 and the outer tube 811 are hollow tubes, one end of the outer tube 811 is provided with an internal thread, and the diameter of the section with the external thread is slightly smaller than the diameter of the middle section of the outer tube 811 and the section with the internal thread. The inner tube 812 has the same diameter as the small diameter of the outer tube 811. The sample part 81 is further provided with a sealing member 813, the sample storage solution is sealed in the cavity of the inner tube 812 by the sealing member 813 and the sample addition part 82 before the reaction, and the sealing member 813 is removed when the sample to be measured is put therein.

In this embodiment, the external thread of the sample addition cap 822 and the internal thread of the outer tube 811 are screwed into the cavity between the outer tube 811 and the inner tube 812 of the sample addition part 82, and the concave plug 821 is located in the inner tube 812 and seals the inner tube 812. The internal thread of the outer sleeve 831 of the reaction part 83 is in threaded connection with the external thread of the outer tube 811, and the end of the inner tube 812 at the connection extends outwards to form a limiting ring, so as to limit the position of the inner tube 812 in the outer tube 811 and prevent the sample preserving fluid or the sample fluid from entering the outer tube 811 into the compartment of the inner tube 812.

In this embodiment, the sample adding part 82 is further provided with a tearing ring 823, and the tearing ring 823 is used for limiting rotation of the sample adding part 82. Tearing ring 823 is located outer tube 811 of sample portion 81 and between application of sample cap 822, adopts the point formula of breaking to connect to tearing ring 823 outwards extends and is equipped with the operation section of being convenient for to tear. The outer circumference of one end of the cap 822 is provided with a plurality of ribs for facilitating the rotary pressurizing operation. The outer circumferences of the sample part 81 and the reaction part 83 are also provided with a plurality of friction ribs for easy operation during rotation.

Before the detection, the quick detection device of the invention firstly pre-embeds the reaction systems in the reaction cavity 833 respectively, the reaction systems are in a freeze-drying state, the reaction systems are pre-embedded in the reaction cavity through an isolation layer (such as paraffin) which can be dissolved by heat, and the sample preservation solution is encapsulated in the cavity of the inner tube 812 in the sample part 81. The sample to be tested is sampled to dip secretion or saliva, the reaction part and the sample part are opened, the sealing piece is removed, the swab section with the sample is arranged in the sample part 81 of the reactor, the reaction part 83 and the sample adding part 82 are screwed down, the tearing pull ring 823 is torn off, the sample adding part 82 is rotated to pressurize the sample liquid, and the sample liquid is forced to flow into the reaction cavity 833 through the micropores 8342. Then placing the reactor 8 into a bearing cavity position 11 of the isothermal amplification instrument, covering a cover, then starting to heat the reactor by a heating part, heating to 60-65 ℃, and keeping the constant temperature for 10 minutes; the vibration part works to make the sample liquid and the reaction system vibrate, mix uniformly and react; and after the reaction, the reactor is placed into a photographing box to photograph the developed reactor.

The above embodiments are merely preferred embodiments of the present invention, which is not intended to limit the present invention in any way. Although the present invention has been described with reference to the preferred embodiments, it is not intended to be limited thereto. Therefore, any simple modification, equivalent change and modification made to the above embodiments according to the technical spirit of the present invention should fall within the protection scope of the technical scheme of the present invention, unless the technical spirit of the present invention departs from the content of the technical scheme of the present invention.

Claims

1. The equipment for quickly detecting the nucleic acid is characterized by comprising an isothermal amplification instrument, wherein the isothermal amplification instrument comprises a bearing part, a heating part, a mounting frame body and a shell, and the bearing part and the heating part are arranged in the shell through the mounting frame body; the bearing part is provided with at least one bearing cavity position for placing a sample to be tested; the heating part is positioned below the bearing part and electrically heats the bearing part.

2. The apparatus for rapid nucleic acid testing according to claim 1, wherein the heating part comprises a heating plate and a plurality of heating pads, the heating pads are located below the heating plate, and the heating plate is fixed to the bottom of the support part.

3. The apparatus for rapid nucleic acid detection according to claim 1, wherein the isothermal amplification device further comprises a vibration unit disposed under the carrier unit and configured to vibrate the carrier unit, and a driving unit configured to drive the vibration unit.

4. The nucleic acid rapid detection device according to claim 1, further comprising a reactor, wherein the sample to be detected is placed in the reactor; the reactor comprises a sample adding part, a sample part and a reaction part which are connected in sequence, wherein the sample adding part, the sample part and the reaction part are movably connected to realize a sealing state; the connecting part of the reaction part and the sample part is provided with micropores, and the aperture of each micropore is not more than the capillary length of the sample liquid or the sample preservation liquid; under the sealed state of the reactor, the sample adding part moves towards the reaction part by external force to realize that the sample liquid passes through the micropores and is pressed into the reaction part under the sealed state.

5. The apparatus for rapid nucleic acid detection according to claim 4, wherein at least one reaction chamber is disposed in the reaction portion, an independent reaction system is pre-disposed in each reaction chamber, so that simultaneous detection of multiple detection items for the same sample can be achieved, the microwells are disposed at the openings of the reaction chambers, and each reaction chamber corresponds to one microwell.

6. The nucleic acid rapid detection device according to claim 5, wherein the reaction chambers are closed at ports close to the sample part, each reaction chamber is in an independent sealing state before reaction, and the ports are closed by a sealing plug or a sealing film.

7. The nucleic acid rapid detection device according to claim 6, wherein the reaction portion is provided with a shunt plug, the shunt plug is located at a joint of the reaction portion and the sample portion, the reaction chamber is independently sealed by the shunt plug, and the micro-hole is formed in the shunt plug.

8. The apparatus for rapid nucleic acid testing according to claim 4, wherein the pore size of the microwell is 0.3 to 0.6mm.

9. The apparatus for rapid nucleic acid detection according to claim 4, wherein the sample-adding part comprises a concave plug and a sample-adding cap, the sample-adding cap is provided with a rigid protrusion for inserting the concave plug into the sample-adding cap, and the sample-adding cap is further provided with a threaded connection section for engaging with the sample part.

10. The apparatus for rapid nucleic acid detection according to claim 1, further comprising a photographing section for photographing the developed sample; the portion of shooing sets up in the shell through the installation support body, the portion of shooing includes the camera, shoots the box, it is equipped with the trench of placing reaction back sample to shoot the box, it can stretch out or retract in the shell to shoot the box.