AU2021100838A4 - Sample Solution Tube and Detection Device - Google Patents

Abstract

The present disclosure provides a sample solution tube and a detection device. At least one first protrusion, a circular truncated cone-like side wall and an external thread are arranged on an outer surface of a side wall of the sample solution tube. The present disclosure further provides a detection device including the sample solution tube, which is provided with a liquid storage slot, a liquid sealing piece, chromatographic test paper, and a puncturing mechanism in sequence from bottom to top; an upper surface of the liquid storage slot is provided with an opening; the opening is sealed by the liquid sealing piece; and the liquid sealing piece is openable. By the adoption of the technical solutions, a sealing effect can be better guaranteed; and moreover, the sample solution tube is in threaded connection with a housing of the detection device, so that the connection structure is stabler. After a detection result is recorded, the liquid sealing piece can be opened, and then the chromatographic test paper can react with a nucleic acid destroying reagent to completely remove nucleic acid remaining in the detection device. 100 140 260 261 240 210 270 200 262 252 251 280 2512 230 220 Fig. 3 100 140 260 111 20\ 261 22 40210 270 20 25 1 25224 262 280 2512 230 220 Fig. 4 100 270 260 200 230 Fig. 5 2

Description

140

260

261 240 210 270 200 262 252 251

280 2512

230 220 Fig. 3

100

140

260 111 20\ 261 22 40210 270 20 25 1 25224 262

280 2512

230 220 Fig. 4

100 270

260

200

230

Fig. 5

Sample Solution Tube and Detection Device

TECHNICAL FIELD

[0001] The present disclosure relates to the field of detection, and in particular, a sample

solution tube and a detection device.

BACKGROUND

[0002] Nucleic acid diagnosis is one of the most dynamic subdivision fields in the future IVD

(in vitro diagnosis) industry. The increase in prevention and control of infectious diseases, the

promotion of blood screening nucleic acid detection and the development of individualized

medicine in China are main drivers for the development of domestic nucleic acid diagnosis.

Driven by these factors, the future growth rate of the domestic nucleic acid diagnosis will be

between 25% and 30%, which obviously exceeds the average growth rate of the domestic IVD

industry. On one hand, nucleic acid diagnosis benefits large medical centers to enable early,

rapid, specific, and high-throughput detection for pathogens, genetic diseases and the like.

[0003] POCT (point-of-care testing) is an emerging subdivision industry of IVD, and is a new

method, which performs immediate analysis at a sampling site, eliminates complicated

handling procedures for specimens in laboratory testing, and obtains test results quickly. The

main standard of the POCT is that there is no need for a fixed detection place, and reagents and

instruments are portable and can be operated in time. The POCT assumes the functions of a

laboratory without the need for traditional hospital laboratory equipment, and can provide all

round services 24 hours to patients without time and place restrictions.

[0004] However, these nucleic acid amplification methods have the problem of easy cross

contamination of amplification products, and false positive signals generated by product

contamination will cause false interpretation of detection results. Cross-contamination between samples is often seen during target nucleic acid amplification operations. The contamination may come from known or unknown positive substances introduced during treatment of negative samples, which cause false positive reactions through air pollution or aerosols.

[0005] A series of methods have been developed in the prior art to prevent the cross

contamination of the amplification products. For example, Reference 1 (CN105199940A)

discloses an anti-pollution portable gene detection method and device. By means of the device,

after a PCR tube containing amplification products is put into the device for sealing, the PCR

tube is punctured to achieve detection. Therefore, contamination of nucleic acid amplification

products is prevented, and false positives are avoided. However, in this device, since the PCR

tube is placed in the device and sealed, the puncturing operation is more difficult, and the

amplification products after the test are still left in the device. Once the tube is damaged, the

amplification products may also be diffused into air, causing false positive reactions.

[0006] In addition, Reference 2 (CN203241416U) also discloses a closed chromatographic test

paper plastic cassette, and Reference 3 (CN205574438U) also discloses a sealed test tube

assembly with a tube puncturing mechanism. These detection devices are more convenient to

operate, but amplification products will also remain in the devices after the detection. There is

a risk of contamination.

[0007] Therefore, providing a nucleic acid detection device with less possibility of

contamination before and after detection has become a problem urgently needing to be solved

in this field.

SUMMARY

[0008] The present disclosure is directed to solve the problems, in the prior art, that a closed

detection device is inconvenient to operate and is still possibly polluted after detection. In order

to solve the above-mentioned problems, the present disclosure discloses a closed sample solution tube of a brand-new structure, and a closed detection device. The device is convenient to operate, and can effectively avoid amplification products from being diffused in air to cause false positive reactions.

[0009] The present disclosure discloses a sample solution tube, including a side wall, a bottom

wall and an upper wall, and a sealed sample solution storage cavity encircled by the side wall,

the bottom wall and the upper wall. The side wall includes an upper side wall, a middle side

wall and a lower side wall which are coaxial and sequentially connected; the upper side wall

and the lower side wall are cylindrical, and the diameter of the upper side wall is greater than

the diameter of the lower side wall; the middle side wall is of a circular truncated cone, and has

a diameter which gradually decreases from an end connected to the upper side wall to an end

connected to the lower side wall; one or more first protrusions are arranged on an outer surface

of the middle side wall; and an external thread is arranged on an outer surface of the lower side

wall.

[00010] By the adoption of the above-mentioned technical solution, a detection process

is easier to operate, and amplification products would leak less possibly. A slope is fitted to a

first channel to better ensure a sealing effect; moreover, since the sample solution tube is in

threaded connection with a housing of the detection device, the connection structure is stabler

and not easy to fall off to further avoid pollution possibility caused by exposure of the

amplification products.

[00011] According to another specific embodiment of the present disclosure, the upper

part of the sample solution tube is of an openable structure or a closed structure.

[00012] According to another specific embodiment of the present disclosure, a sealing

ring made of an elastomer is arranged outside the middle side wall of the sample solution tube.

[00013] The present disclosure further discloses a detection device, including a housing

and any one of the foregoing sample solution tubes, wherein:

[00014] an accommodating cavity is formed in the housing; a liquid storage slot, a liquid

sealing piece, chromatographic test paper, and a puncturing mechanism are arranged in

sequence in the accommodating cavity from bottom to top; an upper surface of the liquid

storage slot is provided with an opening; the opening is sealed by the liquid sealing piece and

the liquid sealing piece is openable;

[00015] an upper surface of the housing is provided with a first channel, and the first

channel is configured to allow the sample solution tube to be inserted to seal the

accommodating cavity; the first channel is provided with a slope matched with the middle side

wall of the sample solution tube; an annular first groove matched with each first protrusion is

formed in the slope; and when the sample solution tube is inserted into the first channel, the

first protrusion and the first groove cooperate with each other to restrain the sample solution

tube from moving in an axial direction relative to the first channel; and

[00016] the puncturing mechanism includes a cylindrical positioning portion, and a

puncturing portion located on a center axis of the positioning portion; the puncturing

mechanism is also provided with a fluid channel; a third limit portion is also arranged at a

position, below the slope, on the first channel; a fourth limit portion is arranged on the

positioning portion; the third limit portion and the fourth limit portion cooperate with each

other to restrain the puncturing mechanism from rotating; an internal thread is arranged on an

inner surface of the positioning portion; an external thread cooperating with the internal thread

is arranged on an outer surface of the sample solution tube; and the sample solution tube is

rotated to cause the puncturing mechanism to move upwards to puncture the sample solution

tube, and a sample solution flows from the fluid channel into the accommodating cavity.

[00017] By the adoption of the above technical solution, in a detection process, the

accommodating cavity is kept sealed all the time, thereby avoiding the amplification products

from leaking to the outside. Much further, after a detection result is recorded, the liquid sealing

piece can be opened, and then the chromatographic test paper can react with a nucleic acid

destroying reagent to completely remove nucleic acid remaining in the detection device. As

such, no pollution will be caused even if the sample solution tube falls off carelessly or the

detection device is broken to expose the interior in subsequent processes. The sample solution

tube is allowed to be kept stationary in the axial direction, but rotated to cause the puncturing

mechanism to move upwards to puncture the sample solution tube. In the puncturing process,

air pressure in the housing does not change, and aerosol of the amplification products will not

leak to the outside, so that pollution can be better avoided. Furthermore, the sample solution

tube is in threaded connection with the housing, so that the connection structure is stabler and

not easy to fall off to further avoid pollution possibility caused by exposure of the amplification

products. Moreover, the nucleic acid destroying reagent is finally used to remove the nucleic

acid remaining in the detection device to further avoid possible pollutions in the subsequent

processes.

[00018] According to another specific embodiment of the present disclosure, the third

limit portion is a second groove extending along the axial direction, and the fourth limit portion

is a second protrusion.

[00019] According to another specific embodiment of the present disclosure, the liquid

sealing piece is capable of moving to open the liquid storage slot; the lower end of the

positioning portion is provided with a third protrusion; a corresponding third groove is formed

in the liquid sealing piece; and the third protrusion is arranged in the third groove before the

puncturing mechanism moves upwards.

[00020] According to another specific embodiment of the present disclosure, an elastic pressing structure is arranged above the chromatographic test paper; and the elastic pressing structure presses at least part of the chromatographic test paper into the liquid storage slot after the liquid sealing piece is opened.

[00021] According to another specific embodiment of the present disclosure, an inserted

end of the first channel is provided with a sealing ring made of an elastomer, or a sealing ring

made of an elastomer is arranged outside the side wall of the sample solution tube.

[00022] According to another specific embodiment of the present disclosure, a

transparent region is also arranged on the housing to observe a detection result.

[00023] According to another specific embodiment of the present disclosure, the nucleic

acid destroying reagent is stored in the liquid storage slot.

BRIEF DESCRIPTION OF THE DRAWINGS

[00024] The present disclosure is further described in detail below in combination with

accompanying drawings and specific embodiments;

[00025] Fig. 1 is a cutaway view of a sample solution tube provided by the present

disclosure;

[00026] Fig. 2 is a schematic structural diagram of a sample solution tube provided by

the present disclosure;

[00027] Fig. 3 is a cutaway view of a sample solution tube and a detection device

provided by the present disclosure in one cooperation state;

[00028] Fig. 4 is a cutaway view of a sample solution tube and a detection device

provided by the present disclosure in another cooperation state;

[00029] Fig. 5 is a schematic structural diagram of a sample solution tube and a detection

device provided by the present disclosure in one cooperation state;

[00030] Fig. 6 is an exploded diagram of a sample solution tube and a detection device provided by the present disclosure;

[00031] Fig. 7 is a schematic structural diagram of a puncturing mechanism provided by

the present disclosure; and

[00032] Fig. 8 is a schematic structural diagram of a puncturing mechanism provided by

the present disclosure.

[00033] Numerals in the drawings:

100 Sample solution tube

110 Side wall

111 Upper side wall

112 Middle side wall

113 Lower side wall

114 First protrusion

120 Bottom wall

121 Recess

130 Upper wall

140 Sample solution storage cavity

150 Sealing ring

200 Housing

210 Accommodating cavity

220 Liquid storage slot

230 Liquid sealing piece

240 Chromatographic test paper

250 Puncturing mechanism

251 Positioning portion

2511 Fourth limit portion

2512 Third protrusion

252 Puncturing portion

253 Fluid channel

260 First channel

261 First groove

262 Third limit portion

270 Transparent region

280 Elastic pressing structure

DETAILED DESCRIPTION

[00034] Particular specific embodiments illustrate the implementation modes of the

present disclosure, and those skilled in the art can easily understand other advantages and

effects of the present disclosure from the content disclosed in this description. Although the

description of the present disclosure will be introduced in conjunction with the preferred

embodiments, this does not mean that the features of the present disclosure are limited to this

implementation mode. On the contrary, the purpose of introducing the present disclosure in

combination with the implementation modes is to cover other options or modifications that

may be extended based on the claims of the present disclosure. In order to provide a deep

understanding of the present disclosure, the following description will contain many specific

details. The present disclosure can also be implemented without using these details. In addition,

in order to avoid confusing or obscuring the focus of the present disclosure, some specific

details will be omitted in the description. It should be noted that the embodiments in the present

disclosure and features in the embodiments may be combined with each other without conflicts.

[00035] In the description of the present embodiment, it should be noted that orientations

or positional relationships indicated by the terms "upper", "lower", "inside", "bottom" and the

like are orientations or positional relationships as shown based on the drawings, or orientations

or positional relationships where the product of the present disclosure is usually placed during

use, and are only for the purpose of facilitating and simplifying the description of the present disclosure instead of indicating or implying that devices or elements indicated must have particular orientations, and be constructed and operated in the particular orientations, so that these terms cannot be construed as limiting the present disclosure.

[00036] The terms "first", "second", "third", etc. are only for the purpose of

distinguishing descriptions, and may not be understood as indicating or implying the relative

importance.

[00037] In the description of the present embodiment, it should be also noted that unless

otherwise explicitly defined and defined, the terms "arranged", "connected" and "connection"

shall be understood broadly, and it may be, for example, fixedly connected, or detachably

connected, or integrally connected, or mechanically connected, or electrically connected, or

directly connected, or indirectly connected through an intermediate medium, or internal

communication between two elements. Those of ordinary skill in the art can understand the

specific meanings of the above terms in the present disclosure according to specific situations.

[00038] In order to make the objectives, the technical solutions and the advantages of

the present disclosure clearer, detailed descriptions will be made to the implementation modes

of the present disclosure below in combination with the accompanying drawings.

[00039] As shown in Fig. 1 and Fig. 2, the present disclosure discloses a sample solution

tube 100, including a side wall 110, a bottom wall 120 and an upper wall 130, and a sealed

sample solution storage cavity 140 encircled by the side wall 110, the bottom wall 120 and the

upper wall 130. The side wall 110 includes an upper side wall 111, a middle side wall 112 and

a lower side wall 113 which are coaxial and sequentially connected; the upper side wall 111

and the lower side wall 113 are cylindrical, and the diameter of the upper side wall 111 is

greater than the diameter of the lower side wall 113; the middle side wall 112 is of a circular

truncated cone, and has a diameter which gradually decreases from an end connected to the

upper side wall 111 to an end connected to the lower side wall 113.

[00040] One or more first protrusions 114 are arranged on an outer surface of the middle

side wall 112. If there are a plurality of first protrusions 114, the plurality of first protrusions

114 can be located on the same cross section of the middle side wall 112; an external thread

(not shown in the figure) is arranged on an outer surface of the lower side wall 113; and the

external thread is located below the first protrusions 114.

[00041] Wherein, the structure of each first protrusion 114 is not particularly restrained

as long as the first protrusion 114 can cooperate with a first groove 261 to restrain a position

of the sample solution tube 100 in an axial direction relative to a first channel 260. The first

protrusion 114 may be a convex point, or may be a convex bar extending along a

circumferential direction, or is an intact circular convex ring. From the perspective of

restraining the position of the sample solution tube 100 along the axial direction more

effectively and stably, the first protrusion 114 is preferably an intact circular convex ring, or a

plurality of convex points or convex bars that are uniformly distributed along the

circumferential direction of the side wall 110.

[00042] According to another specific embodiment of the present disclosure, as shown

in Fig. 1 and Fig. 2, the first protrusion 114 on the middle side wall 112 of the sample solution

tube 100 is a circular convex ring; the lower side wall 110 of the sample solution tube 100

below the first protrusion 114 is a cylindrical side wall; and this cylindrical side wall is provided

with an external thread that cooperates with an internal thread of a positioning portion 251 of

a puncturing mechanism 250.

[00043] According to another specific embodiment of the present disclosure, the sample

solution tube 100 in the present disclosure is a specially-made PCR tube. The side wall 110 of

the sample solution tube 100 may be made of a series of materials, and is preferably made of a

material, such as metal, alloy, heat conduction plastic and an organic composite material, which

has good heat conductivity, high strength and good flowability, is 1 to 3 cm in height, preferably

2 cm, and is approximately shaped like an ordinary PCR tube, but with some differences.

[00044] According to another specific embodiment of the present disclosure, in order to

facilitate the sample solution tube 100 to be punctured by the puncturing mechanism 250 after

being inserted into a detection device, a shear mark can be formed on the bottom wall 120 of

the sample solution tube 100; the bottom wall 120 of the sample solution tube 100 is fractured

along the position of the shear mark when the puncturing mechanism 250 punctures the bottom

wall 120 of the sample solution tube 100; or, the bottom wall 120 can be set to be thinner

relative to other parts of the sample solution tube 100 to facilitate the puncturing mechanism

250 to puncture the sample solution tube 100.

[00045] Further, since the bottom wall 120 of the sample solution tube 100 is provided

with the shear mark or the bottom wall 120 is set to be thinner, the sample solution tube 100

may be possibly broken along the shear mark or the thinner position during fetching and

placement before insertion into the detection device. Therefore, according to another specific

embodiment of the present disclosure, the bottom wall 120 of the sample solution tube 100 is

provided with one recess 121, and the shear mark is arranged in the recess 121; or only the tube

wall of the sample solution tube 100 in the recess 121 is thinner. Preferably, at least the center

position of the bottom wall 120 of the sample solution tube 100 is thinner, so that breakage of

the sample solution tube 100 during fetching and placement can be effectively avoided.

[00046] According to another specific embodiment of the present disclosure, a general

sample solution tube 100 is of an openable structure. That is, the upper wall 130 is an openable

cover. After a sample to be amplified and a reagent related to an amplification reaction system

are placed into the sample solution tube 100, the sample solution tube 100 is covered with the

cover to realize sealing. In addition, the upper part of the sample solution tube 100 may also

directly be of a closed structure. That is, the upper wall 130 and the side wall110 are connected

fixedly, even directly integrated, and cannot be opened. During use, an injector with a fine needle punctures the upper wall 130 of the sample solution tube 100 to inject a reaction system, and a sealing film or a wax drop with a higher melting point then seals the punctured opening.

As such, sealing of the sample solution tube 100 can be better realized.

[00047] Further, for some poor or backward areas, due to poverty, poor sanitation, low

hygiene awareness, malnutrition, etc., they have been hardest hit areas for infectious diseases.

The incidence of infectious diseases is high, the mortality rate is high, and it is hard for ordinary

families to afford the high cost of treatment. However, in these areas, advanced infectious

disease inspection methods cannot be popularized. The main reasons are that most areas have

difficulty in power supply, cannot operate large-scale instruments, cannot afford the cost of

large-scale medical equipment and corresponding maintenance equipment, and are restrained

by sites, and patients cannot afford high inspection fees. An amplification reaction in the

sample solution tube 100 needs to be carried out in a particular temperature range. Testing

personnel can hardly get thermostatic equipment in those areas and cannot carry out detection

on site, thereby restraining the instantaneity of nucleic acid detection.

[00048] According to another specific embodiment of the present disclosure, the surface

of the sample solution tube 100 is coated with at least two reversible thermochromic materials.

Color changing temperatures of the thermochromic materials can be set according to

requirements of actual situations, and specifically, the reversible thermochromic materials can

use commercially available products. For a certain amplification reaction, if there is a need for

a reaction temperature between a first temperature Ti and a second temperature T2, the surface

of the sample solution tube 100 can be selected to be coated with two thermochromic materials.

The color changing temperature of the first thermochromic material is the first temperature TI,

and the color changing temperature of the second thermochromic material is the second

temperature T2. As such, during this amplification reaction, when the first thermochromic

material changes color, and the second thermochromic material does not change color, it is

indicated that the temperature is just suitable for carrying out the amplification reaction in the sample solution tube 100. As such, the sample solution tube 100 can be directly put into a thermos bottle, and the volumes of cold water and hot water are adjusted to control the temperature of water in the thermos bottle to maintain the amplification reaction without thermostatic equipment, and a sample can be detected anytime and anywhere.

[00049] For example, if an optimal reaction temperature is about 38 DEG C, there may

be two temperatures for thermochromic coatings, respectively greater than 38 DEG C and less

than 38 DEG C, preferably 37 DEG C and 39 DEG C. If the optimal reaction temperature is

63 DEG C, the temperatures of the thermochromic coatings can be selected as 62 DEG C and

64 DEG C. The thermochromic coatings may be coated in any shapes, but it is preferably an

Arabic numeral corresponding to the temperature. For example, a thermochromic material

which changes color at 38 DEG C is displayed as "38". As such, the temperature of the sample

solution tube 100 can be reflected more directly.

[00050] Further, when the sample solution tube 100 is applied to various amplification

reaction systems with different temperatures, various thermochromic materials may be

provided.

[00051] According to another specific embodiment of the present disclosure, as shown

in Fig. 3 to Fig. 6, the present disclosure further discloses a detection device, including a

housing 200 and any one of the aforementioned sample solution tubes 100, wherein the

accommodating cavity 210 is formed in the housing 200; a liquid storage slot 220, a liquid

sealing piece 230, chromatographic test paper 240, and a puncturing mechanism 250 are

arranged in sequence in the accommodating cavity 210 from bottom to top; an upper surface

of the liquid storage slot 220 is provided with an opening; the opening is sealed by the liquid

sealing piece 230; and the liquid sealing piece 230 is openable. A nucleic acid destroying

reagent such as a sodium hypochlorite solution or a commercial DNA decontaminant can be

stored in the liquid storage slot 220.

[00052] An upper surface of the housing 200 is provided with a cylindrical first channel

260, and the first channel 260 is configured to allow the sample solution tube 100 to be inserted;

and the shape of the first channel 260 is matched with the sample solution tube 100 to seal the

accommodating cavity 210; the first channel 260 is provided with a slope matched with the

middle side wall 112 of the sample solution tube 100; an annular first groove 261 matched with

each first protrusion 114 is formed in the slope; and when the sample solution tube 100 is

inserted into the first channel 260, the first protrusion 114 and the first groove 261 cooperate

with each other to restrain the sample solution tube 100 from moving in an axial direction

relative to the first channel 260.

[00053] As shown in Fig. 7 and Fig. 8, the puncturing mechanism 250 includes a

cylindrical positioning portion 251, and a puncturing portion 252 located on a center axis of

the positioning portion 251; the puncturing mechanism 250 is also provided with a fluid

channel 253; the first channel 260 is provided with a third limit portion 262; a fourth limit

portion 2511 is arranged on the positioning portion 251; the third limit portion 262 and the

fourth limit portion 2511 cooperate with each other to restrain the puncturing mechanism 250

from rotating around a center axis of the positioning portion 251 serving as a rotating shaft; an

internal thread (not shown) is arranged on an inner surface of the positioning portion 251; and

an external thread cooperating with the internal thread is arranged on an outer surface of the

sample solution tube 100.

[00054] When the sample solution tube 100 is inserted into the first channel 260 and

then rotated, as shown in Fig. 3, the sample solution tube 100 can only rotate due to the

cooperation between the first protrusion 114 and the annular first groove 261, and cannot be

inserted inwards relative to the first channel 260. Furthermore, with the rotation of the sample

solution tube 100, since the external thread on the side wall 110 of the sample solution tube

100 and the internal thread on the positioning portion 251 cooperate with each other, the

positioning portion 251 may drive the puncturing portion 252 to move towards the sample solution tube 100 to a position shown in Fig. 4 (in Fig. 4, the liquid sealing piece 230 has been opened, but the liquid sealing piece 230 cannot be opened before the detection is completed.

Fig. 4 is only used for illustrating a relative positional relationship between the puncturing

mechanism 250 and the sample solution tube 100 here) to puncture the sample solution tube

100, and a sample solution flows from the fluid channel 253 into the accommodating cavity

210. The chromatographic test paper 240 in the accommodating cavity 210 can detect the

sample, and detection personnel can observe a detection result by means of a detection result

transparent region 270.

[00055] By the adoption of the above technical solution, in a detection process, the

accommodating cavity 210 is kept sealed all the time, thereby avoiding amplification products

from leaking to the outside. Much further, after the detection result is recorded, as shown in

Fig. 4, the liquid sealing piece 230 can be opened, and then the chromatographic test paper 240

can react with a nucleic acid destroying reagent to completely remove nucleic acid remaining

in the detection device. As such, no pollution will be caused even if the sample solution tube

100 falls off carelessly or the detection device is broken to expose the interior in subsequent

processes.

[00056] In addition, in a method, in the prior art, for downwards inserting the sample

solution tube 100 and keeping the puncturing mechanism 250 stationary, since air in the

housing 200 is compressed, there might be amplification products leaking from a connection

gap between the sample solution tube 100 and the housing 200, causing pollutions. By means

of the closed detection device of a brand-new structure provided by the present disclosure, the

device allows the sample solution tube 100 to be kept stationary in the axial direction, but

rotated to cause the puncturing mechanism 250 to move upwards to puncture the sample

solution tube 100. In the puncturing process, air pressure in the housing 200 does not change,

and aerosol of the amplification products will not leak to the outside, so that pollution can be

better avoided. Furthermore, the sample solution tube 100 is in threaded connection with the housing 200, so that the connection structure is stabler and not easy to fall off to further avoid pollution possibility caused by exposure of the amplification products. Moreover, the nucleic acid destroying reagent is finally used to remove the nucleic acid remaining in the detection device to further avoid possible pollutions in the subsequent processes.

[00057] According to another specific embodiment of the present disclosure, the third

limit portion 262 is a second groove extending along the axial direction, and the fourth limit

portion 2511 is a second protrusion. The second protrusion may be a convex point, or a convex

bar extending along the axial direction. Furthermore, a length of the second groove needs to

allow the puncturing mechanism 250 to move upwards to a position where the sample solution

tube 100 is punctured. Before the sample solution tube 100 is inserted into the positioning

portion 251 and starts to rotate, the second protrusion is located at the bottommost end of the

second groove. As the sample solution tube 100 rotates, the whole puncturing mechanism 250

moves upwards, and the second protrusion moves upwards along the second groove till the

puncturing mechanism 250 punctures the bottom wall 120 of the sample solution tube 100.

[00058] Further, in a more reliable view, a plurality of second protrusions and second

grooves that cooperate with the second protrusions and extend along the axial direction can

also be provided. Preferably, the plurality of second protrusions and the second grooves that

cooperate with the second protrusions and extend along the axial direction are uniformly

distributed along a circumferential direction of the first channel 260.

[00059] It can be easily thought that the third limit portion 262 may also be set as a

second protrusion, and the fourth limit portion 2511 may also be set as a second groove

extending along the axial direction.

[00060] According to another specific embodiment of the present disclosure, as shown

in Fig. 3 to Fig. 6, a through hole is formed in the housing 200; the liquid sealing piece 230 is arranged on the housing 200 in a penetrating manner by means of the through hole; the liquid sealing piece 230 includes an operation portion located outside the housing 200 and a sealing portion located inside the housing 200; and the sealing portion seals the upper surface of the liquid storage slot 220, and the liquid sealing piece 230 has a first position and a second position. When the detection device is not used, the liquid sealing piece 230 is located at the first position shown in Fig. 3; and the liquid sealing piece 230 can be moved to the second position shown in Fig. 4 by means of the operation portion to open the liquid storage slot 220.

In the whole process, the liquid sealing piece 230 and the housing 200 are hermetically

connected all the time, and the accommodating cavity 210 is always in a sealed state, so that

the amplification products would not leak.

[00061] According to another specific embodiment of the present disclosure, the lower

end of the positioning portion 251 is provided with a third protrusion 2512, and a corresponding

third groove is formed in the liquid sealing piece 230. The third protrusion 2512 is arranged in

the third groove before the puncturing mechanism 250 moves upwards. Due to the cooperation

between the third protrusion 2512 and the third groove, the liquid sealing piece 230 is always

at the first position, and cannot be moved to the second position. After the sample solution tube

100 is inserted into the positioning portion 251 and then rotated to lift the puncturing

mechanism 250, the third protrusion 2512 also moves upwards and is separated from the third

groove. At this time, detection personnel can move the liquid sealing piece 230 from the first

position to the second position by means of the operation portion to open the liquid storage slot

220, and the chromatographic test paper 240 can be in contact with destroying liquid in the

liquid storage slot 220.

[00062] According to another specific embodiment of the present disclosure, an elastic

pressing structure 280 is arranged above the chromatographic test paper 240; and the elastic

pressing structure 280 presses at least part of the chromatographic test paper 240 into the liquid

storage slot 220 after the liquid sealing piece 230 is opened. There is no special limitation to the elastic pressing structure 280. The elastic pressing structure may be a spring or an elastic sheet.

[00063] Further, a specific structure that realizes sealing of the accommodating cavity

210 can refer to any existing mode in the prior art, and no repeated description is provided in

the present disclosure. For example, the shape of the first channel 260 can be set to be matched

with the sample solution tube 100. After the sample solution tube 100 is inserted into the first

channel 260, their surfaces are fitted to realize the sealing of the accommodating cavity 210.

According to another specific embodiment of the present disclosure, in order to avoid the

amplification products more effectively from leaking into air, an inserted end of the first

channel 260 may also be provided with a sealing ring made of an elastomer, or a sealing ring

150 made of an elastomer is arranged outside the side wall 110 of the sample solution tube 100.

For example, the sealing ring 150 is arranged on the middle side wall 112 of the sample solution

tube 100.

[00064] According to another specific embodiment of the present disclosure, a

transparent region 270 is also arranged on the housing to observe the detection result.

[00065] In conclusion, by means of the closed detection device of the brand-new

structure provided by the present disclosure, the accommodating cavity is kept sealed all the

time in the detection process, thereby avoiding the amplification products from leaking to the

outside. Much further, the device allows the sample solution tube to be kept stationary in the

axial direction, but rotated to cause the puncturing mechanism to move upwards to puncture

the sample solution tube. In the puncturing process, air pressure in the housing does not change,

and aerosol of the amplification products will not leak to the outside, so that pollution can be

better avoided. Furthermore, the sample solution tube is in threaded connection with the

housing, so that the connection structure is stabler and not easy to fall off to further avoid

pollution possibility caused by exposure of the amplification products. Moreover, the nucleic acid destroying reagent is finally used to remove the nucleic acid remaining in the detection device to further avoid possible pollutions in the subsequent processes.

[00066] Although the present disclosure has been illustrated and described by referring

to some preferred implementation modes of the present disclosure, those of ordinary skill in

the art should understand that the above content is a further detailed description of the present

disclosure in conjunction with specific embodiments and cannot be considered that the specific

implementations of the present disclosure are only limited to these illustrations. Those skilled

in the art can make various changes in forms and details, including several simple deductions

or substitutions, without departing from the spirit and scope of the present disclosure.

Claims (1)

1) A sample solution tube, comprising a side wall, a bottom wall and an upper wall, and a

sealed sample solution storage cavity encircled by the side wall, the bottom wall and the

upper wall, wherein the side wall comprises an upper side wall, a middle side wall and a

lower side wall which are coaxial and sequentially connected; the upper side wall and the

lower side wall are cylindrical, and the diameter of the upper side wall is greater than the

diameter of the lower side wall; the middle side wall is shaped like a circular truncated

cone, and has a diameter which gradually decreases from an end connected to the upper

side wall to an end connected to the lower side wall; one or more first protrusions are

arranged on an outer surface of the middle side wall; and an external thread is arranged on

an outer surface of the lower side wall.

2) The sample solution tube according to claim 1, wherein the upper part of the sample

solution tube is of an openable structure or a closed structure.

3) The sample solution tube according to claim 1, wherein a sealing ring made of an elastomer

is arranged outside the middle side wall of the sample solution tube.

4) A detection device, comprising a housing and the sample solution tube according to any

one of claims 1 to 3, wherein

an accommodating cavity is formed in the housing; a liquid storage slot, a liquid sealing

piece, chromatographic test paper, and a puncturing mechanism are arranged in sequence

in the accommodating cavity from bottom to top; an upper surface of the liquid storage

slot is provided with an opening; the opening is sealed by the liquid sealing piece; and the

liquid sealing piece is openable;

an upper surface of the housing is provided with a first channel, and the first channel is

configured to allow the sample solution tube to be inserted to seal the accommodating cavity; the first channel is provided with a slope matched with the middle side wall of the sample solution tube; an annular first groove matched with each first protrusion is formed in the slope; when the sample solution tube is inserted into the first channel, the first protrusion and the first groove cooperate with each other to restrain the sample solution tube from moving in an axial direction relative to the first channel; and the puncturing mechanism comprises a cylindrical positioning portion, and a puncturing portion located on a center axis of the positioning portion; the puncturing mechanism is also provided with a fluid channel; a third limit portion is also arranged at a position, below the slope, on the first channel; a fourth limit portion is arranged on the positioning portion; the third limit portion and the fourth limit portion cooperate with each other to restrain the puncturing mechanism from rotating; an internal thread is arranged on an inner surface of the positioning portion; an external thread cooperating with the internal thread is arranged on an outer surface of the sample solution tube; and the sample solution tube is rotated to cause the puncturing mechanism to move upwards to puncture the sample solution tube, and a sample solution flows from the fluid channel into the accommodating cavity.

) The detection device according to claim 4, wherein the third limit portion is a second

groove extending along the axial direction, and the fourth limit portion is a second

protrusion.

6) The detection device according to claim 4, wherein the liquid sealing piece is capable of

moving to open the liquid storage slot; the lower end of the positioning portion is provided

with a third protrusion; a corresponding third groove is formed in the liquid sealing piece;

and the third protrusion is arranged in the third groove before the puncturing mechanism

moves upwards.

7) The detection device according to claim 4, wherein an elastic pressing structure is arranged

above the chromatographic test paper; and the elastic pressing structure presses at least

part of the chromatographic test paper into the liquid storage slot after the liquid sealing

piece is opened.

8) The detection device according to claim 4, wherein a sealing ring made of an elastomer is

also arranged in the first channel.

9) The detection device according to claim 4, wherein a transparent region is also arranged

on the housing to observe a detection result.

) The detection device according to claim 4, wherein a nucleic acid destroying reagent is

stored in the liquid storage slot.

Fig. 2 Fig. 1 Drawing

Fig. 5 Fig. 4 Fig. 3

Fig. 7 Fig. 6

Fig. 8