CN113466443A - A test paper box and sample detection device for depositing test paper - Google Patents

A test paper box and sample detection device for depositing test paper Download PDF

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Publication number
CN113466443A
CN113466443A CN202110502586.1A CN202110502586A CN113466443A CN 113466443 A CN113466443 A CN 113466443A CN 202110502586 A CN202110502586 A CN 202110502586A CN 113466443 A CN113466443 A CN 113466443A
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sample
test paper
liquid
detection
channel
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费凤琴
商涛
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Acon Biotech Hangzhou Co Ltd
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Acon Biotech Hangzhou Co Ltd
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1009Characterised by arrangements for controlling the aspiration or dispense of liquids
    • G01N35/1016Control of the volume dispensed or introduced

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention provides a detection device for detecting biological samples, which comprises a first cover plate, a second cover plate and detection test paper, wherein the first cover plate comprises a quantity-controlled sample adding channel, the quantity-controlled sample adding channel is provided with a sample adding port and a liquid outlet, the capacity of the quantity-controlled sample adding channel is at least the minimum liquid dosage required by detection, the quantity-controlled sample adding channel has a capillary action, and when the sucked liquid dosage cannot reach the minimum dosage, the liquid cannot be absorbed by the detection test paper, or when the sample dosage is insufficient, the liquid sample cannot rapidly contact the detection test paper; when the absorbed liquid amount reaches the minimum detection dosage, the absorbed liquid sample is filled in a sample adding channel between the sample adding port and the liquid outlet, so that the liquid sample at the liquid outlet can contact the detection test paper. The invention realizes automatic quantitative sample adding, can be used in the detection fields of physiological index detection, environment detection and the like, and can realize detection in the detection device by using a lateral cross flow detection method or a vertical flow detection method.

Description

A test paper box and sample detection device for depositing test paper
This application is a divisional application of chinese patent application 201610543841.6 (application date 2016, 7, 8).
Technical Field
The invention relates to a detection device for measuring clinical physiological indexes, in particular to a detection device for rapidly measuring biochemical indexes.
Background
Analysis of a sample can generally be divided into two categories, dry chemical analysis and wet chemical analysis. Dry chemical analysis is a method in which a liquid test sample is directly applied to a dry test strip (dry test strip) to cause a specific chemical reaction using the moisture of the test sample as a solvent, thereby performing chemical analysis, as compared with wet chemical analysis. Test strips for dry chemical analysis techniques are typically tested using either a lateral-flow method or a vertical-flow method. The structure of the dry test strip adopting the lateral cross flow method generally comprises a bottom plate made of hard impermeable materials, and a sample pad, a combination pad, a detection pad and an absorption pad are sequentially adhered on the bottom plate from left to right (or from right to left). The structure of a dry test strip using a vertical flow method generally includes, from top to bottom, a sample pad, a filter pad, and a detection pad. The detection of the sample by the dry test paper basically comprises two steps of sample adding and result reading. The dry test strip can be used for sample analysis independently, or the dry test strip can be assembled in a test paper box for sample analysis. The test paper box comprises a sample adding port corresponding to the test paper strip and a result observation window. No matter the test paper strip is used independently or is matched with a test paper box, when the sample is added, an independent sampling device is required to absorb a certain amount of sample in advance, and then the sampling device is dripped on a sample pad of the test paper strip. And the sample dripping amount of the sampling device is controlled by an operator to meet the detected sample usage amount. Therefore, the existing test paper box equipped with the dry test paper strip cannot realize the automatic sampling function, and cannot control whether the sample adding amount is enough to meet the detection requirement, and the judgment is manually carried out by operators.
For example, the detecting device disclosed in chinese patent 201220201209.0 comprises an upper cover, a bottom plate combined with the upper cover, and a test strip disposed between the upper cover and the bottom plate, wherein the upper cover comprises an upper surface, a lower surface, and a sample injection port penetrating through the upper and lower surfaces, and the lower surface of the upper cover is opposite to the test strip. The diameter of the sample loading port is larger, and the distance between the sample loading port and the test paper is very short. The sample adding port designed in this way can enable the added sample to quickly touch the test paper, so that the sample smoothly enters the test paper and detection is completed. However, this design has the disadvantage that when the amount of sample added does not reach the amount required for testing, the sample will still contact and enter the strip, and the tester will also obtain a result. Due to the insufficient sample adding amount, the detection result is not accurate. Since such a test paper cassette does not give a notice of an insufficient amount of sample to be loaded, the examiner cannot know it. This not only wastes test paper, but can lead to a more serious and therefore erroneous diagnostic conclusion.
Disclosure of Invention
The invention provides a test paper box for storing sample detection test paper, which comprises a first cover plate and a second cover plate, wherein a quantity-controlled sample adding channel is arranged on the first cover plate, the quantity-controlled sample adding channel comprises a sample adding opening and a liquid outlet, and the quantity-controlled sample adding channel at least comprises a section of capillary channel near the sample adding opening.
Furthermore, the whole section of the quantity-control sample-adding channel is in a capillary channel structure.
Further, in order to realize quantitative sample application, the capacity of the sample application channel with controlled amount is at least the minimum liquid amount required for detection.
The shape of the sample-loading channel with controlled amount is selected from the shape of a cylinder, a shape of which the liquid outlet is larger than the sample-loading port, or a shape of which the liquid outlet is smaller than the sample-loading port, and can be any other shape as long as the channel can realize the action of a capillary channel.
In one embodiment, the sample injection device further comprises a diversion trench connected with the sample injection port. The more preferable mode is that the end of the diversion trench far away from the sample injection port is provided with a blocking block.
Furthermore, the first cover plate further comprises a sample adding platform, and the sample adding port is positioned in the center of the sample adding platform.
The lower surface department of first apron is provided with the water conservancy diversion structure. In one embodiment, the liquid outlet comprises a pressure-tight region and a flow-guiding region, the flow-guiding region is located between the two pressure-tight regions, and the pressure-tight regions are connected with the flow-guiding structure.
The second aspect of the present invention is to provide a sample detection device, comprising a test paper box and detection test paper placed in the test paper box, wherein the test paper box comprises a first cover plate and a second cover plate, the first cover plate comprises a quantity-controlling sample-adding channel, the quantity-controlling sample-adding channel is provided with a sample-adding port and a liquid outlet, and the quantity-controlling sample-adding channel at least comprises a section of capillary channel near the sample-adding port.
Further, the capacity of the controlled loading channel is at least the minimum liquid dosage required for detection.
Further, the whole section of the quantity-control sample-adding channel is in a capillary channel structure.
In one embodiment, the sample injection device further comprises a diversion trench connected with the sample injection port.
Further, a flow guide structure is arranged on the lower surface of the first cover plate. In one embodiment, the liquid outlet comprises a pressure-tight region and a flow-guiding region, the flow-guiding region is located between the two pressure-tight regions, and the pressure-tight regions are connected with the flow-guiding structure.
The test paper is selected from dry test paper of a lateral cross flow method or dry test paper of a vertical flow method.
The invention has the beneficial effects that: the liquid sample contacting with the sample adding port is automatically sucked into the sample adding channel by the controlled sample adding channel by utilizing the capillary principle, so that the sample adding process of the sample is automatically completed. In another aspect, the volume of the controlled loading channel is designed to be at least the minimum volume of liquid required for detection. When the absorbed liquid amount does not reach the minimum detection amount, the absorbed liquid is kept in the controlled amount sample adding channel and cannot contact the detection test paper due to the capillary action of the controlled amount sample adding channel, and cannot be absorbed by the detection test paper. Therefore, whether the added liquid sample amount can meet the minimum dosage of detection can be effectively controlled and prompted. If the amount of the added sample cannot reach the minimum detection amount, the detection test paper cannot start the test reaction.
Drawings
FIG. 1 is a test device with a first cover and a second cover snapped together.
Fig. 2 is an exploded view of fig. 1.
Fig. 3 is a cross-sectional view in the direction of fig. 1A-a.
Fig. 4 is a first cover structure view of the test paper cassette.
Fig. 5 is a cross-sectional view in the direction of fig. 4B-B.
Fig. 6 is a first cover plate with a flow directing structure.
FIG. 7 is a schematic of a finger with blood from the fingertip in preparation for top loading.
FIG. 8 is a schematic diagram of the sample filling the controlled loading channel when there is a sufficient amount of blood sample.
FIG. 9 is a schematic diagram of the sample not filling the controlled loading channel when the blood sample amount does not meet the detection amount requirement.
FIG. 10 is a schematic of a finger with blood on the fingertip in preparation for sample application from below.
FIG. 11 is a schematic view of loading from below and filling the controlled loading channel with sample.
FIG. 12 is a schematic view of a test device equipped with lateral cross-flow test strips in a test paper cassette.
Fig. 13 is a first cover plate with a pinch area and a bleed area.
Fig. 14 is a bottom view of the cover plate of fig. 13.
Fig. 15 is a cross-sectional view in the direction of fig. 13C-C.
FIG. 16 is a schematic view of a first cover plate with another form of a controlled amount loading channel.
FIG. 17 is a schematic view of a first cover plate without a sample application station.
Detailed Description
As shown in fig. 1 to 17, the test paper cassette for storing the sample test paper includes a first cover plate 2 and a second cover plate 3, which are assembled with each other in use, and the test paper 100 is mounted therein. A quantity-controlled sample adding channel 5 is arranged on the first cover plate 2, and a sample adding port 4 and a liquid outlet 6 are arranged on the quantity-controlled sample adding channel.
The quantity-controlled sample adding channel 5 at least comprises a section of capillary channel near the sample adding port 4, the quantity-controlled sample adding channel 5 can automatically suck a liquid sample contacted with the sample adding port 4 into the capillary channel by utilizing the capillary principle, the sucked liquid sample flows out of the liquid outlet 6 through the quantity-controlled sample adding channel 5 and then is contacted with the detection test paper, and the sample reacts with a reagent on the test paper to obtain a detection result. In the sample adding embodiments shown in fig. 7 to 9 or fig. 10 and 11, the testing personnel does not need to suck the blood from the fingertip into the blood sampling device in advance, but directly places the finger with the blood from the fingertip at the sample adding port 4, and the capillary channel in the sample adding channel 5 with controlled amount will suck the blood into the capillary channel, thereby realizing the process of automatically adding the sample.
The shape of the controlled-loading channel 5 as shown in FIG. 10 is a cylindrical shape with the channel diameter being uniform from top to bottom. The controlled-volume loading channel may also have a shape in which the liquid outlet is larger than the loading port, for example, a truncated cone shape (as shown in FIG. 5) or a trumpet shape (as shown in FIG. 3). In the embodiment shown in FIG. 16, the shape of the controlled-volume loading channel is such that the upper channel 17 near the loading port 4 has a smaller diameter than the lower channel 16, and the diameter of the entire upper channel is the same and the diameter of the entire lower channel is the same. In the embodiment shown in fig. 17, the upper passage 17 and the lower passage 16 are connected by a truncated cone shaped connecting passage 18. The size setting of sample addition port accords with the capillary principle, and the below of sample addition port is provided with capillary channel, inhales liquid sample from the sample addition port through capillary action in the accuse volume sample addition channel. The size of the liquid outlet can be larger than, smaller than or equal to that of the sample port. In a preferred scheme, the liquid outlet contacts with the detection test paper, the size of the liquid outlet is larger than that of the sample adding opening, the contact area of the liquid outlet and the test paper is increased, and the sucked sample can be diffused to the test paper more quickly and uniformly. The volume-controlled sample-adding channel 5 shown in fig. 3 is in a horn shape, and at least the fine upper end of the channel forms a capillary action, so that a liquid sample can be quickly sucked into the channel, the contact area between an increased liquid outlet and a sample-adding area of the test paper is increased, and the speed of the liquid sample entering the test paper can be further increased.
The loading channel of horn shape (fig. 3) or truncated cone shape (fig. 5) has a larger capacity to hold more liquid than the cylindrical loading channel (fig. 10) under the condition that the loading port and the channel have the same length. Therefore, the quantitative sample adding channel in the shape of a circular truncated cone or a horn does not need to be long, and the capacity of the channel can meet the requirement of the minimum dosage of detection. The shorter the channel distance is, the less time the liquid sample reaches the test paper from the sample addition port is, so that the detection time is shortened, and the detection efficiency is high. Any other sample application channel structure which realizes an automatic sample application mode by utilizing a capillary channel belongs to the scope of the invention.
In one embodiment, the capacity of the sample-feeding channel 5 is at least the minimum liquid amount required for detection, i.e. the amount of liquid stored between the sample-feeding port and the liquid-discharging port can satisfy the minimum detection amount. When the absorbed liquid amount does not reach the minimum detection amount, the absorbed liquid is kept in the controlled amount sample adding channel and cannot contact the detection test paper due to the capillary action of the controlled amount sample adding channel, and cannot be absorbed by the detection test paper. At least when the sample volume is insufficient, the liquid sample does not rapidly contact the test strip after the sample is added, so that the detector notices that the added sample volume does not meet the detection volume requirement, and the sample needs to be added again or supplemented. When the absorbed liquid amount reaches the minimum detection dosage, namely the absorbed liquid sample is filled in the sample adding channel between the sample adding port and the liquid outlet, the liquid sample at the liquid outlet can contact the detection test paper, and the sample in the channel is rapidly absorbed onto the sample adding pad by the attraction of the detection test paper to the liquid. In this design, the accuse volume application of sample passageway not only realizes the automatic acquisition of liquid sample, can effective control moreover with the suggestion liquid volume of adding can satisfy the minimum quantity that detects.
In the sample application examples shown in FIGS. 7 to 9, after the subject places a finger with fingertip blood on the sample application port 4, the controlled sample application channel 5 draws fingertip blood into the channel by capillary action. As shown in FIG. 9, when the amount of bleeding from the fingertip of the subject does not reach the amount of blood for detection, the lower surface 103 of the blood 102 sucked into the sample addition channel 5 does not reach the end of the liquid outlet 6 of the sample addition channel. The aspirated liquid is retained in the controlled loading channel 5 by surface tension without contact with the test strip. The test paper which is not contacted with the liquid sample does not have detection reaction, and the test paper still does not change as before the sample adding, so that a detector can know that the added blood volume is not enough, and the sample adding needs to be continued. As shown in fig. 8, the amount of bleeding from fingertip blood of the subject is sufficient, and when the blood amount for detection has been reached, the lower surface of the blood 102 sucked into the sample-adding channel 5 reaches the end of the liquid outlet 6 of the sample-adding channel and contacts the detection test paper, the blood in the sample-adding channel is sucked into the test paper by the suction force of the detection test paper, and the reagent on the test paper reacts with the blood sample to obtain the detection result.
The sample adding port is spaced from the liquid outlet, so that a finger placed on the sample adding port can be prevented from contacting the detection test paper. The sample adding port with the capillary function can also prevent fingers from contacting the test paper because the opening is small.
In the embodiment shown in fig. 1 to 5, the first cover plate 2 further comprises a sample application platform 7 for supporting a blood collection site of a subject, and a finger with fingertip blood can be placed on the sample application platform. The sample addition port 4 connected to the controlled sample addition channel 5 is located at the center of the sample addition stage 7. In one embodiment, the sample addition stage is inclined from the outer periphery to the center. In another embodiment, the sample adding table comprises a diversion trench, the diversion trench radiates from the sample adding port to the periphery, and the diversion trench guides and collects the liquid sample on the table top of the sample adding table to the sample adding port. In one embodiment, the bottom surface 9 of the flow guide groove 8 is gradually reduced from the periphery to the central sample application port, so that the flow of the liquid sample to the sample application port is more facilitated. In another embodiment, the periphery of the flow guide groove 8 is provided with a blocking block 10, which prevents the liquid sample from flowing out of the sample application station from the flow guide groove and polluting the surrounding environment.
In the embodiment shown in fig. 17, the thickness of the cover plate of the first cover plate 2 is the same as the channel length of the controlled amount sample adding channel 5.
As shown in fig. 6, a flow guiding structure 11 is disposed on the lower surface of the first cover plate 2, and a liquid passage 14 is formed between the liquid outlet 6 and the flow guiding structure. The liquid is limited in the liquid channel by the flow guide structure, and a sample flowing out of the liquid outlet flows to the side along the flow guide structure and is transferred to a corresponding area of the test paper. In the embodiment shown in fig. 13 to 15, the discharge opening 6 comprises a pressing zone 12 and a flow-guiding zone 13, which is located between the two pressing zones. The pressing area is communicated with the flow guide structure and is positioned on the same plane with the flow guide structure. The bottom surface of the pressing zone is farther from the bottom surface of the first cover plate than the bottom surface of the drainage zone. The sample flowing from the outlet port flows out of the drainage region and into the liquid channel 14, which further ensures that the sample flows in a prescribed path.
The test paper box further comprises a detection result observation window, and the observation window corresponds to the detection area of the test paper. As shown in fig. 2 and 3, the viewing window 15 is located on the second cover plate 3. As shown in fig. 12, the viewing window 15 is located on the first cover plate.
The sample testing device shown in fig. 1 and 2 includes a test paper box according to the present invention, and a dry test strip 100 of a vertical flow method is placed in the test paper box. The liquid outlet of the test paper box is positioned above the vertical flow dry test paper sample pad. The sample detection device shown in fig. 12 includes a test paper box of the present invention, a lateral cross flow dry test strip 101 is placed in the test paper box, a liquid outlet of the test paper box is located above a lateral cross flow dry test strip sample pad, and a result observation window 15 of the test paper box is located in a result interpretation area of the lateral cross flow dry test strip.
The detection device can be used for detecting a series of physiological and biochemical indexes such as glucose, cholesterol, high-density fatty acid, low-density fatty acid, triglyceride, uric acid, bilirubin, total protein, hemoglobin and ketone bodies. The detection result can be observed and analyzed by human eyes, and can also be read and analyzed by an instrument.

Claims (11)

1. The test paper box for storing the test paper comprises a first cover plate and a second cover plate, and is characterized in that the first cover plate comprises a quantity-control sample-adding channel, the quantity-control sample-adding channel is provided with a sample-adding port and a liquid outlet, the capacity of the quantity-control sample-adding channel is at least the minimum liquid dosage required by detection, the quantity-control sample-adding channel has a capillary action, and when the sucked liquid dosage cannot reach the minimum dosage, the liquid sample cannot be absorbed by the test paper, or when the sample dosage is insufficient, the liquid sample cannot rapidly contact the test paper; when the absorbed liquid amount reaches the minimum detection dosage, the absorbed liquid sample is filled in a sample adding channel between the sample adding port and the liquid outlet, so that the liquid sample at the liquid outlet can contact the detection test paper.
2. The test paper box as claimed in claim 1, wherein the lower surface of the first cover plate is provided with a flow guide structure, a liquid passage is formed between the liquid outlet and the flow guide structure, the liquid outlet further comprises a pressing area and a flow guide area, the pressing area is communicated with the flow guide structure, and the bottom surface of the pressing area is farther away from the bottom surface of the first cover plate than the bottom surface of the flow guide area.
3. The test paper cassette of claim 1, wherein the first cover further comprises a flow guide groove in fluid communication with the sample port.
4. A test paper cassette as claimed in claim 3, wherein the flow guide slot is provided with a blocking piece away from the sample inlet end.
5. The test paper box as claimed in claim 1, wherein the first cover plate further comprises a sample adding platform, and the sample adding port is positioned at the center of the sample adding platform.
6. A test paper cassette as claimed in claim 1, wherein the size of the liquid outlet is larger than the sample inlet.
7. A kind of sample detection device, including the test paper box and test paper put in the test paper box, the said kit includes the first cover plate and second cover plate, characterized by that, the first cover plate includes the controlled sample loading channel, there are sample loading port and liquid outlet on the controlled sample loading channel, the capacity of the controlled sample loading channel is minimum liquid consumption that the detection needs at least, the controlled sample loading channel has capillary action, when the liquid amount sucked does not reach and detects the minimum consumption, can't be detected and tested the paper absorption, or when the sample amount is insufficient, the liquid sample will not contact the test paper rapidly; when the absorbed liquid amount reaches the minimum detection dosage, the absorbed liquid sample is filled in a sample adding channel between the sample adding port and the liquid outlet, so that the liquid sample at the liquid outlet can contact the detection test paper.
8. The detecting device for detecting the rotation of the motor rotor according to the claim 7, wherein the lower surface of the first cover plate is provided with a flow guiding structure, a liquid channel is formed between the liquid outlet and the flow guiding structure, the liquid outlet further comprises a pressing area and a flow guiding area, the pressing area is communicated with the flow guiding structure, and the bottom surface of the pressing area is farther away from the bottom surface of the first cover plate than the bottom surface of the flow guiding area.
9. The test device of claim 7, wherein the first cover plate comprises a channel in fluid communication with the sample port.
10. The detecting device for detecting the rotation of a motor rotor according to claim 7, wherein the first cover plate comprises a sample adding platform thereon, and the sample adding port is positioned at the center of the sample adding platform.
11. The sample testing device of claim 7, wherein the outlet port is larger in size than the sample port.
CN202110502586.1A 2016-07-08 2016-07-08 A test paper box and sample detection device for depositing test paper Pending CN113466443A (en)

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CN202110502586.1A CN113466443A (en) 2016-07-08 2016-07-08 A test paper box and sample detection device for depositing test paper

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CN107064484A (en) * 2017-05-25 2017-08-18 杭州博旭生物技术有限公司 Self-priming Dry-type biochemical detection means
CN113049356A (en) * 2019-12-27 2021-06-29 苏州微木智能系统有限公司 Sample introduction device and detection system
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WO2018006865A1 (en) 2018-01-11

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