CN113075176B - Tumor marker multi-analysis device - Google Patents
Tumor marker multi-analysis device Download PDFInfo
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- CN113075176B CN113075176B CN202110286197.XA CN202110286197A CN113075176B CN 113075176 B CN113075176 B CN 113075176B CN 202110286197 A CN202110286197 A CN 202110286197A CN 113075176 B CN113075176 B CN 113075176B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
Abstract
The invention belongs to the technical field of medical detection equipment, and particularly relates to a tumor marker multi-analysis device. The device comprises an outer shell, wherein a dustproof cover is arranged at the top of the outer shell, the interior of the outer shell is divided into a test paper detection area and a reagent detection area by a longitudinal partition plate, a test paper detection assembly for detecting a tumor marker by a test paper method is arranged in the test paper detection area, and a reagent detection assembly for detecting the tumor marker by a reagent method is arranged in the reagent detection area; the test paper detection assembly comprises a first sample inlet and a test paper placing groove; the reagent detection assembly comprises a second sample inlet, a flow dividing quantitative tube, a plurality of detection devices, a second detection platform, a telescopic device, a controller and a display screen. The device is suitable for test paper detection and reagent detection methods, has wide application range, is carried out automatically in the detection process, can detect different tumor markers simultaneously, realizes multiple detection effects, saves time and has high detection efficiency.
Description
Technical Field
The invention belongs to the technical field of medical detection equipment, and particularly relates to a tumor marker multi-analysis device.
Background
The tumor marker is also called tumor marker, and refers to a substance which is characterized in malignant tumor cells, or is produced by malignant tumor cells in an abnormal way, or is produced by the stimulation response of a host to the tumor, and can reflect the occurrence and development of the tumor and monitor the response of the tumor to treatment. Tumor markers are present in tissues, body fluids and excretions of tumor patients and can be detected immunologically, biologically and chemically.
The tumor marker is detected by serum or urine, and the detection means comprises a reagent detection method and a test paper detection method. The reagent detection method is to mix a reaction reagent with a sample to be detected to generate products with special properties, such as fluorescent products and the like, and detect the products through a detector to finally calculate the content of the tumor marker. The test paper detection method is characterized in that a special substance capable of being combined with a tumor marker is attached to the test paper, a quality control line and a detection line are arranged on the test paper, and after sample injection is carried out from a test paper sample injection area, the content of the tumor marker is judged by observing and comparing the color development degree of the quality control line and the detection line. However, because different tumor markers have different chemical properties and different target substances combined with the tumor markers, the methods can only detect one tumor marker at a time, and cannot detect samples containing more tumor markers at the same time, so that the detection efficiency is low.
Some devices related to tumor marker analysis in the prior art, such as the blood tumor marker analysis device disclosed in chinese patent CN211669207U, include a seat, grooves are formed on the side walls of both sides of the seat, and a rotating shaft is arranged between the groove walls of both grooves; the device of the patent enables the dustproof shells to rotate through the rotating shaft and enables the blood detection device body to be arranged in the storage groove, the two dustproof shells are mutually fixed through the combined action of the first connecting block, the first T-shaped connecting rod, the first spring, the second T-shaped connecting rod, the second connecting block and other components, the blood detection device body is dustproof and protected, and the problems that the blood detection device for general manual detection and analysis is arranged outside for a long time, dust can enter the blood detection device or the blood detection device can be damaged due to accidental impact are effectively solved; however, the patent can not detect multiple tumor markers simultaneously, and the detection efficiency is not improved. For another example, a blood tumor marker analysis device disclosed in chinese patent CN209167322U includes a box body, a control switch set is disposed on one side of an upper surface of the box body, an input end of the control switch set is electrically connected with an output end of an external power supply, a detection box is disposed at a bottom end inside the box body, the number of the detection boxes is five, and the detection boxes are arranged at equal intervals, a guide tube is penetratingly disposed at a top end inside the box body, the number of the guide tube is not less than five, the five guide tubes correspond to the five detection boxes respectively, a bottom end of the guide tube is located right above the detection boxes, a check valve is disposed at an upper portion of the guide tube, and a cylinder is connected to upper portions of the five guide tubes; the device of the patent can stir blood and enable tumor markers in the blood to be uniformly distributed, the blood flows into a plurality of detection boxes, and the detection of various tumor markers can be carried out by putting different types of tumor marker detection cards into the detection boxes, so that various tumor diseases can be primarily diagnosed at one time, and the detection efficiency is improved to a certain extent; however, the device needs to rely on the one-way valve to control the flow of the detection sample, the detection samples such as blood and urine belong to biological pollutants, the detection samples are easily adhered in the one-way valve, so that the one-way valve is difficult to clean and disinfect, the detection samples in the next time are easily polluted by the detection samples in the previous time, and finally the accuracy of the device is reduced.
In view of the above, there is a need to develop a tumor marker analyzer with high detection efficiency, low contamination rate and wide application range.
Disclosure of Invention
In order to solve the technical problems, the invention provides a tumor marker multi-analysis device which is high in detection efficiency, low in pollution rate and wide in application range.
The invention aims to provide a tumor marker multiple analysis device, which comprises an outer shell, wherein a dustproof cover is arranged at the top of the outer shell, the inside of the outer shell is divided into a test paper detection area and a reagent detection area by a longitudinal partition plate, a test paper detection assembly for detecting a tumor marker by a test paper method is arranged in the test paper detection area, and a reagent detection assembly for detecting the tumor marker by a reagent method is arranged in the reagent detection area;
the test paper detection assembly comprises a first sample inlet and a test paper placing groove, the first sample inlet is fixed on the inner wall of the test paper detection area, the test paper placing groove is positioned below the first sample inlet and communicated with the first sample inlet, and the side wall of the test paper detection area and the test paper placing groove are both made of transparent materials;
the reagent detection assembly comprises a second sample inlet, a flow distribution quantitative pipe, a plurality of detection devices, a second detection platform, a telescopic device, a controller and a display screen; the shunting quantifying pipe comprises a step-shaped shunting pipe which is downwards inclined from the second sample inlet, the bottom of each step of the shunting pipe is communicated with a sample passing column with constant volume, the second detection table is arranged at the bottom in the reagent detection area and is positioned below all the shunting quantifying pipes, the table top at the top of the second detection table is in a step shape matched with the shape of the shunting quantifying pipes, one detection device is arranged on each step of the second detection table, the number of the sample passing columns is greater than that of the detection devices, the bottom end of the sample passing column at the lowest position is a blind end and is not connected with the detection devices, the rest sample passing columns are detachably connected with the detection devices, the telescopic device is arranged on the inner bottom wall of the outer shell and positioned in the reagent detection area and is used for adjusting the position of the detection devices; the bottom is equipped with humidity transducer in the appearance post of crossing that is located the lowest, the controller is located on the shell body outer wall, the controller respectively with humidity transducer detection device the telescoping device the display screen is connected, still connect power and switch on the controller.
Preferably, the tumor marker multi-analysis device comprises a light-tight shell, a sample cell with an arc-shaped cross section is arranged in the light-tight shell, the vertical edge of the sample cell is connected with the inner wall of the light-tight shell through a light shielding plate, a light emitter is arranged in the area where the convex surface of the sample cell is located, light of the light emitter irradiates the convex surface of the sample cell, and a detection sensor is arranged in the area where the concave surface of the sample cell is located; the controller is respectively connected with the light emitter and the detection sensor.
Preferably, above-mentioned multiple analytical equipment of tumor marker, be provided with the panel that is in the light in the sample cell concave surface place region, be equipped with the detection mouth on the panel that is in the light, the probe that detects the sensor is located detection mouth department.
Preferably, in the tumor marker multi-analysis device, the light blocking panel is attached to the concave surface of the sample cell.
Preferably, in the tumor marker multi-analysis device, the telescopic device is an electric telescopic rod and is connected between the bottom of the second detection table and the bottom in the reagent detection area; and a puncture needle capable of puncturing the bottom of the sample passing column is arranged at the top of the sample cell.
Preferably, in the multiple tumor marker analysis device, the sample pool is a vacuum pool in which a detection reagent is placed.
Preferably, in the tumor marker multi-analysis device, the expansion device is a plurality of transverse expansion rods mounted on the longitudinal partition plate, and the plurality of transverse expansion rods are respectively connected with the controller; except the lowest sample passing column, the bottoms of the other sample passing columns are movable sealing plates, the side surfaces of the sealing plates are correspondingly fixed on the transverse telescopic rods one by one, the top of the sample pool is provided with an opening, and the opening is positioned right below the corresponding sample passing column.
Preferably, in the multi-analysis device for tumor markers, the test paper detection assembly comprises a first sample inlet, a sample dividing pipe, a branch pipe, a first detection platform and a test paper placing groove; divide the one end of appearance pipe with first introduction port bottom intercommunication, other end slope downwardly extending, the pipe shaft that divides the appearance pipe and its other end all communicate and are equipped with the bleeder, first detection bench set up in bottom in the test paper detection zone, be located all the bleeder below, first detection bench is equipped with the test paper standing groove, the bleeder one-to-one stretch into the relevant position in the test paper standing groove.
Preferably, the test paper placing groove comprises a groove body with a blind end at the bottom, and a permeable layer capable of permeating liquid is arranged at an opening at the upper end of the groove body.
Preferably, in the multi-analysis device for tumor markers, a test paper embedding groove is formed in the bottom of the permeable layer, and an sampling area for embedding detection test paper is arranged in the test paper embedding groove.
Compared with the prior art, the invention has the following beneficial effects:
1. the test paper detection area is internally provided with a test paper detection assembly for detecting the tumor marker by a test paper method, and the reagent detection area is internally provided with a reagent detection assembly for detecting the tumor marker by a reagent method. The test paper detection assembly comprises a plurality of branch pipes and test paper placing grooves, test paper with different tumor marker detection functions is selected, and different tumor markers can be detected through one-time sample introduction. The reagent detection assembly comprises a controller and a plurality of detection devices, and can also detect different tumor markers. The device is suitable for test paper detection and reagent detection methods, has wide application range, is carried out automatically in the detection process, can detect different tumor markers simultaneously, realizes multiple detection effects, saves time and has high detection efficiency.
2. The invention enables the liquid sample to be detected to automatically flow by arranging the sample dividing pipe and the stepped flow dividing quantitative pipe, can finish the detection of various tumor markers by only one-time sample introduction operation, realizes the automatic detection function by arranging the controller and reduces the workload of personnel.
3. According to the invention, the permeable layer is arranged, so that the liquid sample to be detected can enter the sample inlet area of the detection test paper gently, and the probability of test paper pollution caused by sample splashing is reduced.
4. According to the arc-surface-shaped sample cell provided by the invention, the light emitter irradiates on the convex surface of the arc-surface-shaped sample cell, light can enter the sample cell from multiple directions, the emitted light can be prevented from being focused on the detection sensor, and the detection sensor can be used for pertinently detecting the internal change of the sample cell, so that the detection precision is improved.
Drawings
Fig. 1 is a schematic structural view of a tumor marker multi-analysis device of embodiment 1 of the present invention after a dust cap is covered;
fig. 2 is a schematic structural view of the tumor marker multi-analysis device of embodiment 1 of the present invention with a dust cap removed;
FIG. 3 is a schematic structural diagram of a test strip detection assembly according to embodiment 1 of the present invention;
FIG. 4 is a schematic structural view of a test paper holding groove in example 1 of the present invention;
FIG. 5 is a schematic structural view of a reagent measuring assembly according to example 1 of the present invention;
FIG. 6 is a schematic view of a detecting device in embodiment 1 of the present invention;
fig. 7 is a schematic view of a concave structure of a light-blocking panel according to embodiment 1 of the present invention;
FIG. 8 is a block diagram showing the connection of the modules in embodiment 1 of the present invention;
FIG. 9 is a schematic structural view of a reagent detecting unit according to embodiment 2 of the present invention.
Detailed Description
In order that those skilled in the art will better understand the technical solutions of the present invention to be implemented, the present invention will be further described with reference to the following specific embodiments and accompanying drawings.
In the description of the present invention, it is to be understood that the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.
The terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or to implicitly indicate the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature; in the description of the present invention, "a plurality" means two or more unless otherwise specified.
Example 1
The embodiment provides a tumour marker multiple analysis device, refer to fig. 1-2, including shell body 1, shell body 1 top lid closes or articulates there is shield 2, shell body 1 is inside to be cut apart into test paper detection zone 11 and reagent detection zone 12 through longitudinal baffle 3, is provided with the test paper determine module that supplies the test paper method to detect the tumour marker in the test paper detection zone 11, is provided with the reagent determine module that supplies the reagent method to detect the tumour marker in the reagent detection zone 12.
Referring to fig. 3, the test strip detecting assembly includes a first sample inlet 4, a sample dividing tube 41, a branch tube 42, a first detecting platform 43 and a test strip placing groove 44; the first sample inlet 4 is fixed on the inner wall of the outer shell 1 through a first fixing plate, or the first sample inlet 4 is fixed on the longitudinal partition plate 3 through the first fixing plate, the first sample inlet 4 is used for feeding a liquid sample to be detected, one end of the sample dividing pipe 41 is communicated with the bottom of the first sample inlet 4, the other end of the sample dividing pipe extends downwards in an inclined manner, a pipe body of the sample dividing pipe 41 and the other end of the sample dividing pipe are both communicated with branch pipes 42, the number of the branch pipes 42 is at least one, and the sample dividing pipe 41 is arranged in a manner of inclining downwards from the first sample inlet 4, so that the liquid sample to be detected can be quickly divided into the branch pipes 42 under the action of gravity; first test table 43 sets up bottom in the test paper detection zone 11 of shell body 1, is located all bleeder pipes 42 below, is equipped with test paper standing groove 44 on the first test table 43 that detects, and the quantity of test paper standing groove 44 equals with the quantity of bleeder pipes 42, and bleeder pipes 42 one-to-one stretches into the test paper standing groove 44 of relevant position in, and test paper standing groove 44 is used for placing the test paper that can detect the tumor marker. The liquid sample to be tested flowing through the branch pipe 42 enters the test strip placing groove 44 and is detected by the test strip of the corresponding area. The process of detecting the tumor marker by the test paper is completed. The side wall of the outer shell 1 located in the test paper detection area 11 and the test paper placing groove 44 are made of transparent materials, and the detection result can be observed from the outside. Since the present embodiment is provided with a plurality of branch tubes 42 and test paper placement grooves 44, and test paper having different tumor marker detection functions is selected, different tumor markers can be detected by one-time sample injection.
Preferably, referring to fig. 4, the test paper placing groove 44 includes a groove body 441 having a blind end at the bottom, the groove body 441 is a longitudinal groove or an inclined groove, the inside of the groove body 441 is used for placing test paper, a permeable layer 442 capable of permeating liquid is covered or inlaid at an opening at the upper end of the groove body 441, and the permeable layer 442 may be a sponge layer or a sterile cotton layer; the bottom of the permeation layer 442 is in contact with a sample injection region of the detection test paper in the groove body 441, or the bottom of the permeation layer 442 is provided with a test paper embedding groove 443, a sample injection region used for embedding and contacting the detection test paper is arranged in the test paper embedding groove 443, a sample to be detected gradually permeates or chromatographically permeates to the sample injection region of the detection test paper through the permeation layer 442, and further chromatographically permeates to a detection test paper terminal on the detection test paper.
Referring to fig. 5-8, the reagent detecting assembly of this embodiment includes a second sample inlet 5, a split quantitative tube 51, a plurality of detecting devices, a second detecting platform 52, a telescopic device, a controller and a display 53; the second sample inlet 5 is fixed on the inner wall of the outer shell 1 through a second fixing plate, or the second sample inlet 5 is fixed on the longitudinal partition plate 3 through a second fixing plate, the shunt quantitative tube 51 comprises a shunt tube in a step shape downwards inclined from the second sample inlet 5, the shunt tube comprises a plurality of steps, each step bottom of the shunt tube is communicated with a sample passing column with a constant volume, the top surface of the sample passing column is flush with the bottom of each step, so that the sample passing column is filled with a liquid sample to be detected and then flows into the next sample passing column automatically, the bottom of the sample passing column can be punctured by a puncture needle, the second detection table 52 is arranged at the bottom in the reagent detection area 12 of the outer shell 1 and is positioned below all the shunt quantitative tubes 51, the top table surface of the second detection table 52 is in a step shape matched with the shape of the shunt quantitative tubes 51, each step of the second detection table 52 is provided with a detection device, the number of the detection devices is smaller than the number of the sample passing columns, the bottom ends of the sample passing columns at the lowest positions are blind ends, the detection devices are not connected, and the detection devices are detachably connected with the other sample passing columns. On the diapire in shell body 1, be located and be equipped with the telescoping device in reagent detection zone 12, in this embodiment, the telescoping device is used for adjusting detection device's high position, the telescoping device is electric telescopic handle 55, its stiff end is installed on the diapire in shell body 1, be located reagent detection zone 12, the expansion end is installed in the second and is examined test table 52 bottom, height through adjusting detection device, can make shunting quantifying pipe 51 cross appearance post and detection device intercommunication, and will wait to examine liquid sample and transmit to detection device in, utilize detection device to detect the tumour marker wherein, and utilize display screen 53 show testing result.
The principle of detecting the tumor marker by the reagent is that the tumor marker is combined with a detection reagent substance to generate a special biochemical substance which can be detected, such as a special color or a fluorescent property, and the like, and then the detector is utilized to detect the change of the biochemical property, and finally the content of the tumor marker is calculated. Most commonly, the reaction product generates fluorescence after being irradiated by ultraviolet, the fluorescence intensity change is detected by using a fluorescence detection sensor, and the content of the tumor marker is finally calculated, so that the quantification of the tumor marker is realized. Therefore, the structure of the detection device in this embodiment is shown in fig. 6-7, which includes a light-tight shell 54, where the light-tight shell 54 is cylindrical or elliptical, a sample cell 541 with an arc cross section is disposed in the light-tight shell 54, the sample cell 541 is used to receive a liquid sample to be detected in a shunt tube, the interior of the sample cell 541 is vacuum and is added with a detection reagent, a puncture needle is disposed at the top of the sample cell 541, a distance of 1-10cm is left between the outer wall of the sample cell 541 and the inner wall of the light-tight shell 54, the vertical edge of the sample cell 541 is connected with the inner wall of the light-tight shell 54 through a light-shielding plate 542, the light-shielding plate 542 divides the light-tight shell 54 into a light-emitting area and a result-receiving area, the area where the convex surface of the sample cell 541 is located is a light-emitting area, a light emitter 543 is disposed in the light-emitting area, the light-emitter 543 can be an ultraviolet light emitter, a laser emitter, an X-ray emitter, a white light emitter, etc., the light of the light-emitter 543 irradiates the convex surface of the sample cell 541 to make substances in the sample cell exhibit fluorescence or other reactions, the concave surface of the result-receiving area is disposed in the result-receiving area, and a detection sensor is disposed in the result-receiving area; for example, if the light emitter 543 excites the substance in the sample cell 541 with the ultraviolet light emitter to generate fluorescence, the detection sensor is a fluorescence detection sensor; the detection sensor is a color detection sensor if the light emitter 543 uses a white light emitter to illuminate the color change of the substance in the sample cell 541.
The bottom is equipped with humidity transducer in the appearance post of crossing that is located lowest, and the appearance post of crossing of lowest is as cushioning effect, and it does not connect detection device, and the controller is located shell body 1 outer wall, and the controller is connected with humidity transducer, light emitter 543, detection sensor, telescoping device, display screen 53 respectively, still is connected with the power that is used for supplying power for equipment on the controller, still is connected with the switch whether control detection operation goes on the controller. Module attachment figure see figure 8. The specific working principle is as follows: open the switch, then parts such as controller and telescoping device are in waiting operating condition, add the liquid sample of waiting to examine in the second introduction port 5, then under the action of gravity, the liquid sample of waiting to examine flows through each of reposition of redundant personnel dosage tube 51 and crosses the appearance post, because we set up be the reposition of redundant personnel dosage tube 51 of ladder shape, then every is crossed the interior liquid sample volume of waiting to examine that adds of appearance post and is invariable, can set up the different volume of crossing the appearance post according to the demand and be 10 microliters, 50 microliters, 100 microliters, 200 microliters etc., can set up reposition of redundant personnel dosage tube 51 to dismantle the centre gripping on the second fixed plate, change the reposition of redundant personnel dosage tube 51 of different specifications and can realize the appearance demand that need not detect with the reagent. When the humidity sensor in the lowest sample passing column detects a signal, it means that all the sample passing columns above the humidity sensor are filled with the liquid sample to be detected, the humidity sensor transmits the signal to the controller, the controller controls the display screen 53 to display a "full" signal, the sample adding is stopped manually, the redundant liquid sample to be detected in the shunt pipe enters the lowest sample passing column, then the controller controls the expansion device to work, the second detection table 52 and the detection device move upwards, the puncture needle punctures the bottom of the corresponding sample passing column, the liquid sample to be detected enters the sample pool 541 under the vacuum action, after reacting for a period of time t, the controller controls the light emitter 543 to emit corresponding light, then the detection sensor transmits the detected signal to the controller for processing, the final processing result is displayed through the display screen 53, and an operator views the detection result through the display screen 53. Note that the time t is designed according to the chemical reaction requirement, such as 5-60 minutes.
The detection mode of the test paper detection assembly and the reagent detection assembly is suitable for test paper detection and reagent detection methods, the application range is wide, the detection process is automated, different tumor markers can be detected simultaneously, multiple detection effects are achieved, time is saved, and the detection efficiency is high.
According to the arc-surface-shaped sample cell 541 provided by the invention, the light emitter 543 irradiates on the convex surface of the sample cell 541, so that light can enter the sample cell 541 from multiple directions under the results of light scattering, refraction, reflection and the like, even if the penetrating power of the light is strong, the emitted light can be prevented from being focused on the detection sensor, the detection sensor can be used for pertinently detecting the internal change of the sample cell 541, and the detection precision is improved. In order to further improve the detection accuracy, a light-blocking panel 544 is disposed in the result receiving area, preferably, the light-blocking panel 544 is attached to the concave surface of the sample cell 541, a detection port 5441 is disposed on the light-blocking panel 544, and a probe of the detection sensor is located at the detection port 5441, so that interference of the light emitter 543 and light refracted by other positions in the sample cell 541 can be avoided.
Example 2
The multiple tumor marker analysis device of this embodiment, the structure with embodiment 1 is basically the same, the difference lies in that the telescoping device is a plurality of horizontal telescopic links 56, the stiff end of horizontal telescopic link 56 is installed on longitudinal baffle 3, a plurality of horizontal telescopic links 56 are connected with the controller respectively, except the lower sample post of crossing, all the other sample post bottoms of crossing are the mobilizable board of sealing, the side one-to-one of board of sealing is fixed in the expansion end of horizontal telescopic link 56, the top of sample cell 541 is the opening, and this opening is located its corresponding sample post of crossing under. Before the appearance advances, seal the board and be located under the appearance post, and will cross appearance post bottom and seal, make it not weeping, along with advancing the progress of kind process, detect the signal when the humidity transducer who is located the lower appearance post of crossing in the appearance post, then represent that all cross above that the appearance post is filled with in waiting to examine the liquid sample, humidity transducer gives the controller with signal transmission, controller control display screen 53 shows "filling up" signal, then the artifical application of sample that stops, then in the shunt tubes unnecessary waiting to examine the liquid sample gets into the lower appearance post of crossing, afterwards, controller control telescoping device work, make seal the board to 3 direction movements of longitudinal baffle, then cross appearance post bottom and be opened, its inside liquid sample of waiting to examine falls into sample cell 541.
Because some tumor marker detection reagents are added into the sample cell 541 in batches, the structure of the present embodiment does not need the vacuum sample cell 541, and the reagents can be added into the sample cell 541 for multiple times, and after being mixed uniformly, the detection device is placed on the step of the second detection platform 52 to perform the above detection work.
It should be noted that, the connection relation of the components not specifically mentioned in the present invention is the default of the prior art, and the connection relation of the structures is not described in detail since it does not relate to the invention point and is a common application of the prior art.
It should be noted that, when the present invention relates to numerical ranges, it should be understood that two endpoints of each numerical range and any value between the two endpoints can be selected, and since the steps and methods adopted are the same as those in the embodiment, in order to prevent redundancy, the present invention describes a preferred embodiment. While preferred embodiments of the present invention have been described, additional variations and modifications in those embodiments may occur to those skilled in the art once they learn of the basic inventive concepts. Therefore, it is intended that the appended claims be interpreted as including preferred embodiments and all such alterations and modifications as fall within the scope of the invention.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (9)
1. The tumor marker multi-analysis device comprises an outer shell (1), wherein a dustproof cover (2) is arranged at the top of the outer shell (1), and is characterized in that the inside of the outer shell (1) is divided into a test paper detection area (11) and a reagent detection area (12) through a longitudinal partition plate (3), a test paper detection assembly for detecting a tumor marker by a test paper method is arranged in the test paper detection area (11), and a reagent detection assembly for detecting the tumor marker by a reagent method is arranged in the reagent detection area (12);
the test paper detection assembly comprises a first sample inlet (4) and a test paper placing groove (44), the first sample inlet (4) is fixed on the inner wall of the test paper detection area (11), the test paper placing groove (44) is positioned below the first sample inlet (4) and communicated with the first sample inlet (4), and the side wall of the test paper detection area (11) and the test paper placing groove (44) are both made of transparent materials;
the reagent detection assembly comprises a second sample inlet (5), a shunting quantifying pipe (51), a plurality of detection devices, a second detection table (52), a telescopic device, a controller and a display screen (53); the shunting quantifying pipe (51) comprises a step-shaped shunting pipe which is downwards inclined from the second sample inlet (5), the bottom of each step of the shunting pipe is communicated with a sample passing column with constant volume, the second detection table (52) is arranged at the bottom in the reagent detection area (12) and is positioned below all the shunting quantifying pipes (51), the top table surface of the second detection table (52) is in a step shape matched with the shape of the shunting quantifying pipe (51), each step of the second detection table (52) is provided with one detection device, the number of the sample passing columns is greater than that of the detection devices, the bottom end of the sample passing column positioned at the lowest position is a blind end and is not connected with the detection device, the rest sample passing columns are detachably connected with the detection device, the bottom wall in the shell (1) is provided with the telescopic device in the reagent detection area (12), and the telescopic device is used for adjusting the position of the detection device; the bottom is equipped with humidity transducer in the appearance post of crossing that is located the lowest, the controller is located on shell body (1) outer wall, the controller respectively with humidity transducer detection device telescoping device display screen (53) are connected, still connect power and switch on the controller.
2. The tumor marker multiple analysis device according to claim 1, wherein the detection device comprises a light-tight housing (54), a sample cell (541) with an arc-shaped cross section is arranged in the light-tight housing (54), the vertical edge of the sample cell (541) is connected with the inner wall of the light-tight housing (54) through a light-shielding plate (542), a light emitter (543) is arranged in the region where the convex surface of the sample cell (541) is located, light of the light emitter (543) irradiates the convex surface of the sample cell (541), and a detection sensor is arranged in the region where the concave surface of the sample cell (541) is located; the controller is connected with the light ray emitter (543) and the detection sensor respectively.
3. The tumor marker multiplex analysis device according to claim 2, wherein a light-blocking panel (544) is disposed in the region of the concave surface of the sample cell (541), a detection port (5441) is disposed on the light-blocking panel (544), and the probe of the detection sensor is located at the detection port (5441).
4. The tumor marker multiplex analyzer as claimed in claim 3, wherein the light-blocking panel (544) is attached to the concave surface of the sample cell (541).
5. The tumor marker multiplex assay device according to claim 2, wherein the telescoping device is an electric telescoping rod (55) connected between the bottom of the second test platform (52) and the bottom of the reagent test zone (12); and a puncture needle capable of puncturing the bottom of the sample passing column is arranged at the top of the sample cell (541).
6. The tumor marker multiplex analyzer according to claim 5, wherein the sample cell (541) is a vacuum cell in which a detection reagent is placed.
7. The tumor marker multiplex analysis device according to any one of claims 1 to 6, wherein the test strip detection assembly comprises a first sample inlet (4), a sample dividing tube (41), a branch tube (42), a first detection stage (43) and a test strip placement slot (44); divide the one end of appearance pipe (41) with first introduction port (4) bottom intercommunication, other end slope downwardly extending, the pipe shaft that divides appearance pipe (41) and its other end all communicate and are equipped with bleeder (42), first test table (43) set up in bottom in test paper detection zone (11), be located all bleeder (42) below, be equipped with on first test table (43) test paper standing groove (44), bleeder (42) one-to-one stretches into relevant position in test paper standing groove (44).
8. The tumor marker multi-analysis device according to claim 7, wherein the test paper placement groove (44) comprises a groove body (441) with a blind bottom, and a permeable layer (442) permeable to liquid is arranged at an opening at the upper end of the groove body (441).
9. The tumor marker multi-analysis device according to claim 8, wherein a test paper embedding groove (443) is formed at the bottom of the permeation layer (442), and a sample inlet area for embedding a test paper is formed in the test paper embedding groove (443).
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Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU1257297A (en) * | 1996-02-21 | 1997-09-04 | Biomerieux Vitek, Inc. | Automatic sample testing machine |
| CN101031800A (en) * | 2004-09-30 | 2007-09-05 | 爱科来株式会社 | Analyzer |
| CN201673154U (en) * | 2010-04-30 | 2010-12-15 | 艾康生物技术(杭州)有限公司 | Detection apparatus |
| CN206715966U (en) * | 2017-04-28 | 2017-12-08 | 刘玲 | A kind of bioanalysis micro-fluidic detection device |
| CN207826829U (en) * | 2017-12-19 | 2018-09-07 | 北京大学深圳医院 | Urogenital Mycoplasma quick detection kit |
| CN208441190U (en) * | 2018-01-26 | 2019-01-29 | 北京秒康智能科技有限公司 | The intelligent closestool of automatic urine examination |
| CN109669037A (en) * | 2019-02-28 | 2019-04-23 | 深圳市易瑞生物技术股份有限公司 | A kind of Seperated multiple channel chromatographic apparatus |
| CN109813653A (en) * | 2019-01-28 | 2019-05-28 | 台州为尔智能科技有限公司 | A kind of reagent type test device for intelligent closestool |
| CN209167322U (en) * | 2018-11-26 | 2019-07-26 | 张文杰 | A kind of neoplastic hematologic disorder analysis of markers device |
| CN209264735U (en) * | 2018-12-12 | 2019-08-16 | 深圳市易瑞生物技术股份有限公司 | A kind of high pass amount detecting device for two-step method immunochromatography |
| CN111812329A (en) * | 2020-07-28 | 2020-10-23 | 山东圣剑医学研究有限公司 | Antibody chip kit for quantitatively detecting multiple tumor markers |
| CN212031307U (en) * | 2020-03-03 | 2020-11-27 | 江苏一脉科技有限公司 | Online analysis device for excess alkali |
| CN112105912A (en) * | 2018-04-13 | 2020-12-18 | 华盛顿大学 | Method and apparatus for single biological nanoparticle analysis |
| CN212540344U (en) * | 2020-07-22 | 2021-02-12 | 河南省京测检测技术有限公司 | Food safety detection device |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070154895A1 (en) * | 2005-12-30 | 2007-07-05 | Caliper Life Sciences, Inc. | Multi-assay microfluidic chips |
| CA2994889C (en) * | 2009-12-07 | 2019-01-22 | Meso Scale Technologies, Llc | Assay cartridges and methods of using the same |
| WO2012137506A1 (en) * | 2011-04-08 | 2012-10-11 | パナソニック株式会社 | Diagnosis kit and diagnosis method |
| US20160320381A1 (en) * | 2011-09-25 | 2016-11-03 | Theranos, Inc. | Systems and methods for multi-analysis |
| CN107121548A (en) * | 2016-02-25 | 2017-09-01 | 深圳市迈科龙生物技术有限公司 | Quantitatively detect test paper, preparation method and the detection method of tumor markers |
| CN106198950A (en) * | 2016-07-08 | 2016-12-07 | 艾康生物技术(杭州)有限公司 | For depositing paper box and the sample testing apparatus of Test paper |
| SE540437C2 (en) * | 2017-01-13 | 2018-09-18 | Calmark Sweden Ab | Detection of a biomarker in a sample of a flowable substance |
-
2021
- 2021-03-17 CN CN202110286197.XA patent/CN113075176B/en not_active Expired - Fee Related
Patent Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU1257297A (en) * | 1996-02-21 | 1997-09-04 | Biomerieux Vitek, Inc. | Automatic sample testing machine |
| CN101031800A (en) * | 2004-09-30 | 2007-09-05 | 爱科来株式会社 | Analyzer |
| CN201673154U (en) * | 2010-04-30 | 2010-12-15 | 艾康生物技术(杭州)有限公司 | Detection apparatus |
| CN206715966U (en) * | 2017-04-28 | 2017-12-08 | 刘玲 | A kind of bioanalysis micro-fluidic detection device |
| CN207826829U (en) * | 2017-12-19 | 2018-09-07 | 北京大学深圳医院 | Urogenital Mycoplasma quick detection kit |
| CN208441190U (en) * | 2018-01-26 | 2019-01-29 | 北京秒康智能科技有限公司 | The intelligent closestool of automatic urine examination |
| CN112105912A (en) * | 2018-04-13 | 2020-12-18 | 华盛顿大学 | Method and apparatus for single biological nanoparticle analysis |
| CN209167322U (en) * | 2018-11-26 | 2019-07-26 | 张文杰 | A kind of neoplastic hematologic disorder analysis of markers device |
| CN209264735U (en) * | 2018-12-12 | 2019-08-16 | 深圳市易瑞生物技术股份有限公司 | A kind of high pass amount detecting device for two-step method immunochromatography |
| CN109813653A (en) * | 2019-01-28 | 2019-05-28 | 台州为尔智能科技有限公司 | A kind of reagent type test device for intelligent closestool |
| CN109669037A (en) * | 2019-02-28 | 2019-04-23 | 深圳市易瑞生物技术股份有限公司 | A kind of Seperated multiple channel chromatographic apparatus |
| CN212031307U (en) * | 2020-03-03 | 2020-11-27 | 江苏一脉科技有限公司 | Online analysis device for excess alkali |
| CN212540344U (en) * | 2020-07-22 | 2021-02-12 | 河南省京测检测技术有限公司 | Food safety detection device |
| CN111812329A (en) * | 2020-07-28 | 2020-10-23 | 山东圣剑医学研究有限公司 | Antibody chip kit for quantitatively detecting multiple tumor markers |
Non-Patent Citations (1)
| Title |
|---|
| 多种肿瘤标志物联合检测的液相芯片系统的建立;黄晓卉等;《中华实验外科杂志》;20061130;第23卷(第11期);全文 * |
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